Total Tannin Content Research Articles

Effect of gamma irradiation on total phenolic contents and antioxidant activities of methanolic extract of java plum seeds

<p>The synthetic antioxidant butylated hydroxyanisole (BHA) has been widely added to lipids and food products as a preservative due to inhibiting lipid oxidation. However, concerns have arisen about the potential health impacts of this synthetic antioxidant on consumers. Therefore, there is a need for an alternative substitute for BHA that is safe for human health. The methanolic extract of Java Plum seeds (MEJS) contains a diverse group of phenolic compounds and has the potential to serve as a natural antioxidant. The antioxidant must remain stable during food processing, such as irradiation. This research aimed to determine the effect of γ-irradiation on the total phenolic content and antioxidant activity of MEJS. When γ-irradiation was applied to MEJS at doses up to 12.5 kGy, there was a slight decrease in total phenolic and tannin content but a significant increase in total flavonoid content. This increase in total flavonoids led to a rise in both free radical scavenging and reducing power activities</p>

Antimicrobial Activity of Dill Seeds and Celery Seeds on Beef Burger

Medicinal plants possess compounds that can replace the conventional chemical preservatives utilized to preserve meat, which have adverse health effects on consumers. Many biochemical properties of dill and celery seeds as antimicrobial agents have been stated; thus, the present research aimed to determine the effect of employing dill and celery seed extracts as antimicrobial agents in beef burgers. In this study, dill and celery seed were extracted with methanol, ethanol, and acetone. Results showed that maximum yield was obtained from dill and celery seed extracts using methanol (58.4% and 55%). Total phenolic compound (TPC), total flavonoid (TF), total tannin contents (TTC), antioxidant activity, and the fraction of phenolic substances were identified in different extracts. Results showed that dill seed extract has the highest scores of TPC (8.22 mg/GAE/g), TFC (4.99 mgQE/g), and TTC (0.91%). The scavenging effects of dill and celery seed extracts on DPPH radicals were recorded (91.84%) in dill seed extract and (84.12%) in celery seed extracts. Additionally, antibacterial results showed that dill and celery seed extracts inhibited all tested microorganisms (Staphylococcus aureus, E. coli O157:H7, Salmonella typhimurium, Pseudomonas aeruginosa, and Listeria monocytogenes). At concentrations of 0.5%, 1.0%, and 1.5%, dill, and celery seed extracts were individually added to beef burgers as antimicrobials. Furthermore, by reducing the growth of bacteria throughout the preservation period, the treated burger had a substantially longer shelf life than the control sample. However, (TVN) Total volatile nitrogen, (TBA) Thiobarbituric acid, and total plate count in each sample containing dill and celery seed extracts were smaller than the control at zero time and rose over time in every specimen. However, beef burger samples containing extracts of dill and celery revealed a slower rate of increase relative to the control. All the parameters of sensory evaluation of cooked beef burger samples prepared by adding 0.5, 1, and 1.5 % of dill and celery seed extracts were acceptable. Results showed a slightly decreasing taste and flavor value obtained by 1.5 dill and celery seed extracts. Finally, extracts of dill and celery seed could be used as functional ingredients in meat-based foods.

Antioxidant, Anti-Inflammatory and Antiproliferative Effects of Osmanthus fragrans (Thunb.) Lour. Flower Extracts.

Osmanthus fragrans (Thunb.) Lour. flowers (OF-F) have been traditionally consumed as a functional food and utilized as folk medicine. This study evaluated the antioxidant, anti-inflammatory and cytotoxic effects of OF-F extracts on prostate cancer cells (DU-145) and determined possible protein-ligand interactions of its compounds in silico. The crude OF-F extracts-water (W) and ethanol (E) were tested for phytochemical screening, antioxidant, anti-inflammatory, and anti-cancer. Network and molecular docking analyses of chemical markers were executed to establish their application for anticancer drug development. OF-F-E possessed higher total polyphenols (233.360 ± 3.613 g/kg) and tannin (93.350 ± 1.003 g/kg) contents than OF-F-W. In addition, OF-F-E extract demonstrated effective DPPH scavenging activity (IC50 = 0.173 ± 0.004 kg/L) and contained a high FRAP value (830.620 ± 6.843 g Trolox/kg). In cell culture experiments, OF-F-E significantly reduced NO levels and inhibited cell proliferation of RAW-264.7 and DU-145 cell lines, respectively. Network analysis revealed O. fragrans (Thunb.) Lour. metabolites could affect thirteen molecular functions and thirteen biological processes in four cellular components. These metabolites inhibited key proteins of DU-145 prostate cancer using molecular docking with rutin owning the highest binding affinity with PIKR31 and AR. Hence, this study offered a new rationale for O. fragrans (Thunb.) Lour. metabolites as a medicinal herb for anticancer drug development.

Callus Induction and Bioactive Compounds Production from Various Cultivars of Medicago sativa L. (alfalfa)

Alfalfa (Medicago sativa L.) belongs to fabacaea family widely grown in Turkey. It is rich in bioactive compounds such as phenolic compounds, flavonoid, essential amino acids (threonine, leucine, lysine, and valine) and tannins, vitamins (A, B1, B2, B6, B12, C and E) or β-carotene. In this study, it was aimed to investigate the impact of secondary metabolite content of explants on callus biomass. For this purpose, cotyledon explants were obtained under sterile conditions, and transferred to standard MS medium containing 1 mg/L 2,4-D (Dichlorophenoxy Acetic Acid) and 0.0125 mg/L kinetin to induce callus formation. The phenolic, flavonoid and tannin contents of the explants were also determined. Leaves and cotyledons explants of 74 M. sativa L. cultivars have been used for callus biomass. The 74 tested alfalfa cultivars varied in their callus growth and callus biomass formation. Van-22, Konya-Ereğli, Alsancak, Gözlü-1 and Iside cultivars were observed with higher callus biomass: Conversely, Van Gevaş, Bitlis Hizan and Van-Çaldıran responded with lower callus biomass in tissue culture. A high-callus biomass cultivar of alfalfa has been shown to have higher total phenolic, flavonoid and tannin content activity than the lower-callus biomass cultivar in terms of leaf explants under tissue culture conditions. Total phenolic content activity was significantly increased in cotyledon explants with higher callus biomass as compared to lower callus biomass. The accumulation of leaf tannin and flavonoid was strongly linked to callus biomass. Cotyledon phenolic and flavonoid content exhibited an increasing trend in response to the increasing biomass of callus.

Composition and Content of Hydrolysable Tannins in Feijoa Leaves, <i>Acca sellowiana</i>

Introduction. Acca sellowiana, or feijoa belongs to the Myrtaceae family and it is an evergreen shrub or small tree 4–6 meters high. The phytochemical composition has been most thoroughly studied in the fruits of A. sellowiana. It has been shown that feijoa is rich in phenolic acids, hydrolysable and condensed tannins, steroidal saponins, and flavonoids. Hydrolysable tannins are known to have high biological activity.Aim. To study the composition and content of hydrolysable tannins in the leaves of A. sellowiana.Materials and methods. Ultra-high performance liquid chromatography coupled with diode array detection and mass spectrometry.Results and discussion. The results obtained showed the presence of 22 phenolic compounds in the extract of the A. sellowiana leaves. They have been classified as flavan-3-ols, flavonoids and hydrolysable tannins. Two compounds could not be identified. The total content of hydrolysable tannins was 44,28 mg/g (90 % of the total phenolic content). At the same time, ellagitannins were the main phenolic compounds of the extract (40,47 mg/g), while the amount of galloyl-glucose was only 3,81 mg/g.Conclusions. Composition and content of hydrolysable tannins in the leaves of A. sellowiana introduced in the greenhouse of VILAR have been studied. The leaves accumulate mainly monomeric ellagitannins represented 92 % of the total content of hydrolysable tannins. The data obtained indicate that the leaves of A. sellowiana can be used as a raw material for obtaining extracts with a high content of ellagitannins and developing drugs based on them.

Nutritional and phytochemical screening of Moringa oleifera leaf powder in aqueous and ethanol extract

ABSTRACT Moringa oleifera has gained tremendous popularity in recent time due to its unlimited nutraceutical and functional properties. Present study was intended to investigate bioactive compounds present in Moringa oleifera leaf powder (MoLP). Moringa leaves were plucked, dried, ground, and were preliminarily analyzed for their proximate composition and mineral profile. Standard tests were conducted to evaluate flavonoids, alkaloids, saponins, proteins, amino acids, fats, and oils. Total phenolic content (TPC), total flavonoid content (TFC), total saponins content (TSC), total tannins content (TTC), FRAP assay, and DPPH radical scavenging property was also analyzed. Phytochemical investigation of Moringa oleifera leaf powder and its extract depicted the existence of rich bioactive compounds including amino acids, phenols, flavonoids, and saponins. The study illustrated that the phytochemicals in Moringa oleifera exhibit higher antioxidant activity against free radicals, revealing its strong nutritional worth.

Assessing the potential of rosary pea (Abrus precatorius L.) derived aqueous seed extracts as anticancer agents and their phytoconstituents as COX-2 inhibitor: an in-vitro and in-silico approach

Abrus precatorius L. is a traditional remedy with a long history of use in medicine around the globe due to its diverse phytochemical composition and bioactivities which are of utmost significance to the scientific community. With the aim to provide new insights into the antioxidant, antiproliferative and antiangiogenic properties of A. precatorius aqueous seed extracts, different extraction methods were employed. Aqueous extract prepared by Soxhlet method APW (Sox) had higher total phenolics, flavonoids and tannin content. In DPPH assay, APW (Sox) had the maximum free radical scavenging activity. The maximum FRAP value was displayed by APW (Mac). The maximum inhibition was shown by APW (Sox) against HPV18 (Hep2C) cells and APW (Mac) against HPV18 (KB) cells. In cervical cancer (Hep2C) cells, catalase (CAT), glutathione-s-transferase (GST) activity, and glutathione (GSH) content were all highest in APW (Sox) extract, whereas APW (Mac) extract demonstrated the highest superoxide dismutase (SOD) activity and the lowest malondialdehyde (MDA) content. Similarly, in oral cancer (KB) cells, APW (Mac) extract showed the highest SOD, CAT, GST activity and GSH content whereas APW (Sox) extract showed the least MDA content. Docking studies showed that tannic acid and rutin had the highest binding affinity, while MD simulations showed that they were stable in complex with COX-2 for at least 90 ns. Promising antiangiogenic activities were observed in both APW (Sox) and APW (Mac) in a dose dependent manner. Therefore, aqueous seed extracts of A. precatorius could be considered promising candidates for anticancer and antiangiogenic drugs. Communicated by Ramaswamy H. Sarma

Melatonin supplementation combats nickel-induced phytotoxicity in Trigonella foenum-graecum L. plants through metal accumulation reduction, upregulation of NO generation, antioxidant defence machinery and secondary metabolites

Nickel (Ni) at a toxic level (80 mg kg−1 of soil) adversely affects the crop performance of fenugreek (Trigonella foenum-graecum L.). Melatonin (MEL), a potent plant growth regulator, is ascribed to offer promising roles in heavy metal stress alleviation. In this study, different doses viz. 0, 25, 50, 75 and 100 μM of MEL were administered to plants through foliage under normal and Ni-stress conditions. The experiment unveiled positive roles of MEL in enhancing root-shoot lengths, fresh-dry weights, seed yield and restoring photosynthetic efficiency assessed in terms of higher Fv/Fm, YII, qP, and lower NPQ values in plants exposed to Ni (80 mg kg−1). MEL supplementation (at 75 μM) effectively restricted Ni accumulation and regulated oxidative stress via modulation of MDA, O2−, H2O2 and NO generation, most prominently. Besides, MEL at 75 μM more conspicuously perked up the activities of antioxidant enzymes like SOD, POX, CAT and APX by 15.7, 20.0, 14.5 and 16.5% higher than the Ni-exposed plants for effective ROS scavenging. Likewise, MEL at 75 μM also efficiently counteracted Ni-generated osmotic stress, through an upscaled accumulation of proline (19.6%) along with the enhancement in the concentration of total phenols (13.6%), total tannins (11.2%), total flavonoids (25.5%) and total alkaloids (19.2%) in plant's leaves. Furthermore, under 80 mg kg−1 Ni stress, MEL at 75 μM improved the seed's trigonelline content by 40.1% higher compared to Ni-disturbed plants, upgrading the pharmacological actions of the plant. Thus, the present study deciphers the envisaged roles of MEL in the alleviation of Ni stress in plants to enhance overall crop productivity.

Insight into biological activities of chemically characterized extract from Marrubium vulgare L. in vitro, in vivo and in silico approaches.

Aqueous extracts of Marrubium vulgare L. (M. vulgare) are widely used in traditional medicine for their therapeutic effects. Hence, this study aims to evaluate in vitro, in vivo, and in silico the biological activities of M. vulgare aqueous extract to further support their traditional use. Qualitative phytochemical tests of M. vulgare extracts showed the presence of primary and secondary metabolites, while quantitative analyses recorded revealed the contents of total phenols, flavonoids, and tannins, with values of 488.432 ± 7.825mg/EAG gallic acid extract/g, 25.5326 ± 1.317mg/EQ Quercetin extract/g and 23.966 ± 0.187mg/EC catechin extract/g, respectively. Characterization of the phytochemical constituents of the extract revealed the presence of catechin and maleic acid as the most abundant while the evaluation of the antioxidant power revealed that the extract possesses significant antioxidant capacity, antimitotic potential, and antimicrobial properties against Streptococcus agalactiae and Staphylococcus epidermidis among many others. The antidiabetic activity of the extract showed a potent antihyperglycemic effect and a significant modulation of the pancreatic α-amylase activity as revealed by both in vitro and in vivo analysis, while an in silico evaluation showed that chemicals in the studied extract exhibited the aforementioned activities by targeting 1XO2 antimitotic protein, W93 antidiabetic protein and 1AJ6 antimicrobial protein, which revealed them as worthy of exploration in drug discovery odyssey. Conclusively, the result of this study demonstrates the numerous biological activities of M. vulgare and gives credence to their folkloric and traditional usage.

Effect of some local plant extracts on fatty acid composition of fish (Alestes baremoze) during smoking and sun drying in the Far-North region of Cameroon.

The objective of this study was to assess the antioxidant activities of three plant extracts (Moringa oleifera leaves, Xylopia aethiopica fruits, and Allium cepa leaves) and to evaluate their effects on the preservation of fish polyunsaturated fatty acids (PUFAs) during smoking and sun-drying processes. PUFAs are highly prone to oxidation during fish processing. The plant extracts were analyzed for their polyphenol contents and were evaluated for their total antiradical capacity. The polyphenol components of each plant were characterized. The hydroethanolic and aqueous extracts were added to the fish at concentrations of 3, 6, 9, and 12 g/L and 10, 20, 30, and 40 g/L, respectively. Butylated hydroxytoluene (BHT) was used as a positive control at a concentration of 2 g/L to compare the antioxidant effects of the plant extracts. The treated fish was subjected to smoking or sun drying and the fatty acid composition of the fish lipid extract was assessed. The results showed that the total polyphenolic, flavonoid, and tannin contents varied significantly from one plant extract to the other (p < .05). The radical scavenging and FRAP increased significantly with the concentration of the plant extracts (p < .05). An HPLC analysis of the extracts led to the preliminary identification of four hydroxycinnamic acids in M. oleifera and X. aethiopica, one anthocyanin and one flavone glycoside in M. oleifera, and four flavan-3-ols in X. aethiopica. Moreover, eight flavonols were preliminarily identified in the three plants. Compared to the control product, these plant extracts significantly protected fish PUFAs from oxidation (p < .05). The aqueous extract of A. cepa at 40 g/L better preserved omega-3 in fish during smoking and sun drying than the control product. Incorporating the three plant extracts during smoking and sun-drying processes can effectively preserve the PUFAs in fish. Therefore, these plants are viable sources of natural antioxidants in the preservation of fish products.

Exploring the potentials of sorghum genotypes: a comprehensive study on nutritional qualities, functional metabolites, and antioxidant capacities.

Sorghum, long regarded as one of the most underutilized crops, has received attention in recent years. As a result, conducting multidisciplinary studies on the potential and health benefits of sorghum resources is vital if they are to be fully exploited. In this study, the nutritional contents, functional metabolites, and antioxidant capacities of 23 sorghum breeding lines and three popular cultivars were assessed. All of the sorghum genotypes were grown under the same conditions, and mature seeds were hand-harvested. The metabolite contents and antioxidant capacities of sorghum seeds were assessed using standard protocols. Fatty acids were quantified using a gas chromatography-flame ionization detector, whereas flavonoids and 3-deoxyanthocyanidins were analyzed using a liquid chromatography-tandem mass spectrometry method. The data were analyzed using both univariate and multivariate statistical approaches. Total protein (9.05-14.61%), total fat (2.99-6.91%), crude fiber (0.71-2.62%), dietary fiber (6.72-16.27%), total phenolic (0.92-10.38 mg GAE/g), and total tannin (0.68-434.22 mg CE/g) contents varied significantly across the sorghum genotypes (p < 0.05). Antioxidant capacity, measured using three assays, also differed significantly. Five fatty acids, including palmitic, stearic, oleic, linoleic, and linolenic acids, were found in all the sorghum genotypes with statistically different contents (p < 0.05). Furthermore, the majority of the sorghum genotypes contained four 3-deoxyanthocyanidins, including luteolinidin, apigeninidin, 5-methoxyluteolinidin, and 7-methoxyapigeninidin, as well as two dominant flavonoids, luteolin and apigenin. Compared to the cultivars, some breeding lines had significantly high levels of metabolites and antioxidant activities. On the other hand, statistical analysis showed that total tannin, total phenolic, and antioxidant capacities varied significantly across white, yellow, and orange genotypes. Principal component analysis was used to differentiate the sorghum genotypes based on seed color and antioxidant index levels. Pearson's correlation analysis revealed strong links between biosynthetically related metabolites and those with synergistic antioxidant properties. This research demonstrated the diversity of the sorghum resources investigated. Those genotypes with high levels of nutritional components, functional metabolites, and antioxidant activities could be used for consumption and breeding programs.

TinyML-Sensor for Shelf Life Estimation of Fresh Date Fruits.

Fresh dates have a limited shelf life and are susceptible to spoilage, which can lead to economic losses for producers and suppliers. The problem of accurate shelf life estimation for fresh dates is essential for various stakeholders involved in the production, supply, and consumption of dates. Modified atmosphere packaging (MAP) is one of the essential methods that improves the quality and increases the shelf life of fresh dates by reducing the rate of ripening. Therefore, this study aims to apply fast and cost-effective non-destructive techniques based on machine learning (ML) to predict and estimate the shelf life of stored fresh date fruits under different conditions. Predicting and estimating the shelf life of stored date fruits is essential for scheduling them for consumption at the right time in the supply chain to benefit from the nutritional advantages of fresh dates. The study observed the physicochemical attributes of fresh date fruits, including moisture content, total soluble solids, sugar content, tannin content, pH, and firmness, during storage in a vacuum and MAP at 5 and 24 ∘C every 7 days to determine the shelf life using a non-destructive approach. TinyML-compatible regression models were employed to predict the stages of fruit development during the storage period. The decrease in the shelf life of the fruits begins when they transition from the Khalal stage to the Rutab stage, and the shelf life ends when they start to spoil or ripen to the Tamr stage. Low-cost Visible-Near-Infrared (VisNIR) spectral sensors (AS7265x-multi-spectral) were used to capture the internal physicochemical attributes of the fresh fruit. Regression models were employed for shelf life estimation. The findings indicated that vacuum and modified atmosphere packaging with 20% CO2 and N balance efficiently increased the shelf life of the stored fresh fruit to 53 days and 44 days, respectively, when maintained at 5 ∘C. However, the shelf life decreased to 44 and 23 days when the vacuum and modified atmosphere packaging with 20% CO2 and N balance were maintained at room temperature (24 ∘C). Edge Impulse supports the training and deployment of models on low-cost microcontrollers, which can be used to predict real-time estimations of the shelf life of fresh dates using TinyML sensors.

ANTIOXIDANT AND Alfa-AMYLASE INHIBITION ACTIVITIES OF MEDICINAL PLANTS ARE TRADITIONALLY USED FOR THE TREATMENT OF DIGESTIVE DISORDERS IN THE NEAR EAST REGION

Medicinal plants have been used for longtime for treatment of many illnesses. Many plants were described in folk heritage to have anti-inflammatory activity to treat digestive diseases. In this study, thirteen plants commonly used by Jordanian folks to treat digestive disorders were investigated to find a connection between their contents and activities and their relationship with their assigned folk use. The ethanolic extracts of the plants were tested for their Total Phenolic Content (TPC), Total Tannin Content (TTC), Total Flavonoid Content (TFC) and their antioxidants activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). In addition to α-Amylase inhibitory activity of the plant extracts was determined. Correlations between TPC, TFC, TTC, protein content with the antioxidant activities (DPPH and ABTS), α-amylase inhibition were determined using Pearson correlation. The phenolic content and flavonoid content showed a high correlation with ABTS activity. The studied plants varied from activation to inhibition of α-Amylase, this may benefit people with hypoglycemia. This diversity of values directs people to which plant to use in case of multi-case disease.

Phytochemical screening of Zanthoxylum armatum roots and exploring its polyphenol content and antioxidant activity

The objective of the article is to investigate the chemical composition of Zanthoxylum armatum roots methanolic extract and to evaluate the polyphenol content and antioxidant potential. Gas Chromatography-Mass Spectroscopy (GC-MS) and High-Performance Liquid Chromatography – Photo Diode Array (HPLC-PDA) techniques were used to characterize chemical composition of methanolic extract of roots. The GC-MS analysis extract resulted in the isolation of thirty-seven phytochemical constituents in methanol extracts. The root extracts were strongly characterized by 1-Propene, 2-nitro-3-(1-cyclooctenyl), (2E,4E)-N-Isobutyldodeca-2,4-dienamide, (+)- Seasmin and Paulowin. The phenolic composition of the root extract quantified by using HPLC revealed Ascorbic acid as most abundant polyphenol, followed by Gallic acid, Chlorogenic acid, Epigallocatechin gallate, Vanillic acid and p-hydroxybenzoic acid, while Caffeic acid was the recorded as least. In addition, root extract also showed the presence of antioxidant activity (DPPH Free radical scavenging, Metal Chelating and Ferric Reducing Antioxidant Activity) and polyphenol content (Total Phenol, Flavonoid, and Tannin Content).

Infant food formulations based on cereals (millet, maize) and caterpillar powder (Imbrasia oyemensis): Nutritional composition and sensory evaluation

Malnutrition is a real public health problem, particularly in low-income households. Therefore, the objective of this work is to improve nutritional quality of complementary foods based on maize and millet flours intended for children during diversification period of low-income households. Thus, biochemical and phytochemical composition of ten cereal-based formulations (maize and millet) incorporated or not with Imbrasia oyemensis caterpillar powder were studied using standard and conventional methods. In addition, these formulations were subjected to a hedonic test by a panel of fifty people. It was found that incorporation of Imbrasia oyemensis powder improved contents of protein (11.97±0.03-16.4±0.03%), lipids (7.65±0.49-11.08±0.20%), ash (2.07±0.05-3.20±0.05%) and energy value (402.97±13.9- 483.63±35.71 kcal/100g) of maize (FMa0) and millet (FMi0) based flours. In addition, these formulations showed excellent contents of total polyphenols (160.19±2.80-256.16±0.00 mg/100g), flavonoids (10.95±0.07-25.64±0.15mg/100g) and tannins (109.45±0.68- 225.54±0.90 mg/100g). In addition, anti-nutritional contents such as phytates (97.24±0.08-128.60±0.08 mg/100g) and oxalates (215.81±1.67-370.65±9.29 mg/100g) are below the acceptable limit in human food. Thus, these incorporated formulations of Imbrasia oyemensis caterpillar powder were highly appreciated by panelists. Incorporation of Imbrasia oyemensis powder in maize and millet-based supplementary foods constitutes a cheaper and healthier alternative in children diet during diversification period of low-income households in Côte d'Ivoire.

Evaluation of Photoprotective of Gyrinops versteegii (Gilg.) Domke Leaves Extract with Different Solvents and Its Spatial Distribution

&lt;p&gt;&lt;em&gt;&lt;span lang="EN-US"&gt;Gyrinops versteegii &lt;/span&gt;&lt;/em&gt;&lt;span lang="EN-US"&gt;(Gilg.) Domke is one of the Thymelaeaceous family species with high economic value. The leaf synthesizes secondary metabolites (SMs) supporting herbal medicine, including a class of SMs that serve as sunscreen. However, the SMs are not equally distributed in the plant, depending on the organs, tissues or cell type. This research aimed to determine the spatial distribution of secondary metabolites in the leaves, especially in the epidermis and mesophyll tissues. The epidermis tissues were separated from the mesophyll using the &lt;em&gt;carborundum abrasion &lt;/em&gt;(CA) technique, followed by the extraction of SMs using three different polarity solvents, namely methanol, hexane and chloroform. The photoprotective potential was evaluated using the SPF value with the spectrophotometry approach. Subsequently, the phytochemical analysis was done for the total tannin, flavonoid, and phenols content. The results showed that the highest SPF value was observed in the methanol epidermis extract with SPF value of 8.61, followed by methanol mesophyll extract (4.52), chloroform epidermis extract (3.97), chloroform mesophyll extract (2.52) and hexane epidermis extract (0.60) and hexane mesophyll extract (0.16). Total phenolic content (flavonoid, tannin and phenol) in epidermis extract with methanol solvent was higher (12.347 ± 0.652 mg GAE/g DW), followed by extract with chloroform and hexane solvents. The total phenolic content (flavonoid, tannin and phenol) of mesophyll extract with methanol solvent showed the highest content, followed by chloroform mesophyll and hexane extracts. The results of the histochemical analysis showed that photoprotective compounds accumulate in the epidermis and mesophyll tissue of the leaf of &lt;em&gt;G. versteegii&lt;/em&gt;.&lt;/span&gt;&lt;/p&gt;

Biochemical, Antimicrobial, and Antioxidant activities of some wild Mushrooms from Nepal

Wild mushrooms represent a crucial dietary staple for many tribal groups throughout the world since they consist of a significant source of bioactive constituents such as phenolic compounds, tocopherol, and act as anti-cancer, anti-allergic, anti-obesity, anti-inflammatory compounds, etc. Wild mushrooms including Scleroderma citrinum, Heterobasidion annosum, Coriolus hirsutus, Cavimalum indicum, Russula sanguinea, and Suillus punctatipes were studied to evaluate their phytochemical constituents, antimicrobial activity, antioxidant activity, toxicity and its importance as a source of food along with safety concerns. Initially, the total flavonoid content (TFC), total phenolic content (TPC), and total tannin content (TTC) along with antioxidant, and antimicrobial activity were assessed using ethanolic extracts of fungus. Furthermore, a Brine shrimp bioassay was performed, the correlation of which with antioxidant activity, TPC, TFC, TTC, and lethal concentration (LC50) value was shown by principal component analysis (PCA). Secondary metabolites like glucosides, flavonoids, polyphenols, alkaloids, terpenoids, saponins, and quinones were identified using phytochemical investigations. The TPC ranged from 45.98 to 102.3 mg GAE/g for the extracts, TFC from 100 to 225 mg QE/g, and the TTC was found to vary between 80 to 180 mg GAE/g. The findings of the antioxidant studies demonstrated that S. punctatipes exhibited the highest antioxidant activity (IC50 = 16.95 µg/mL), followed by C. indicum (IC50 = 22.5 µg/mL), and C. hirsutus (IC50 = 35.34 µg/mL). Likewise, S. punctatipes exhibited strong antimicrobial activity as compared to other extracts. The larvicidal efficacy against brine shrimp tests revealed that three mushrooms; C. hirsutus, C. indicum, and S. punctatipes—contain highly toxic substances while the other three are non-toxic and when properly examined can be consumed to some extent.

Phytochemical Analysis and Evaluation of the Antioxidant and Anti-Inflammatory activity of Canavalia gladiata

The present study was done to determine whether scimitar bean or sword bean (Canavalia gladiata) seeds that had been stored for a year contained any proximate phytochemical equivalents and to assess their antioxidant and anti-inflammatory properties. In order of increasing polarity, hexane, ethyl acetate, and methanol were employed to extract phytochemicals from sword bean seeds. Hexane, ethyl acetate, and methanol extract each had a phytochemical yield of 0.23%, 0.26%, and 1.46%, respectively. Quantitative analysis of the extracts revealed that methanol extract had the highest levels of total polyphenolic contents (TPC) (17.74±1.929mg of gallic acid equivalents/g DM) and total tannin contents (TTC) (49.94±1.94mg of tannic acid equivalents/g DM), while hexane extract had the highest levels of total flavonoid contents (TFC) (9.06±1.197mg of quercetin equivalents/g DM). In C. gladiata seed extracts, tannins made up the majority of all phytochemicals. The solvent extract from C. gladiata seeds also showed strong antioxidant activity as measured by the total antioxidant and DPPH assays. Based on the inhibitory concentration (IC50) value of the DPPH assay, the ethyl acetate extract was shown to be the most effective antioxidant of all extracts (12.68±0.027µg/ml). Anti-inflammatory properties of extracts were evaluated by the egg albumin denaturation method, heat-induced and hypo-tonicityinduced HRBC membrane stabilization methods. The results of the heat-induced HRBC membrane stabilization method with an IC50of 613.39±0.975 µg/ml and hypo-tonicity induced HRBC membrane stabilization method with an IC50of 185.91±11.008 µg/ml revealed that ethyl acetate extract has significantly higher anti-inflammatory activity whereas methanol extract has shown higher anti-inflammatory activity as per egg albumin denaturation method with an IC50of 636±3.51µg/ml. Results showed that C. gladiata seed contains varying levels of phytochemical equivalents and indicate that the antioxidant and anti-inflammatory potential varied significantly and have a potential comparable to the standard drugs, ascorbic acid and Butylated Hydroxytoluene (BHT). Hence, C. gladiata seeds that have been stored for a year could be a good source of phytochemicalsand they can be used in pharmaceutical, cosmetic, and other formulations.

Antibacterial Activity of Eight Medicinal Plants from the Traditional Pharmacopoeia of Niger.

The emergence of multidrug bacterial resistance poses a great public health problem and requires a constant search for new antibacterial agents. However, Niger's flora possesses several medicinal plants used in traditional medicine to cure infectious diseases and can be used as sources of bioactive ingredients. This current study was designed to evaluate the antibacterial activity of eight plants used in the traditional pharmacopeia of Niger. The extracts were prepared by maceration using ethanol, methanol, and distilled water. The obtained extracts were screened against Salmonella spp., Shigella spp., and Escherichia coli using the microdilution method coupled with a resazurin-based assay. Phytochemical screening was performed using colorimetry, while the quantification of total polyphenols, total flavonoids, and total tannins was determined by spectrophotometry. Out of the eight plants obtained, five named Cassia italica, Limeum pterocarpum, Phyllanthus pentandrus, Strychnos innocua, and Ximenia americanum exhibited antibacterial activity with MICs ranging from 500 μg/mL to 2000 μg/mL. Phytochemical screening showed the presence of alkaloids, saponosides, tannins, flavonoids, terpenes/sterols, quinones, and polyphenols. The ethanolic and methanolic extracts of X. americana contained important quantities of total polyphenols, with 43.59 ± 0.15 and 41.97 ± 0.02 mg EAG/100 mg of extract, respectively. These extracts showed the highest contents of total tannins at 46.49 g/L and 45.52 g/L, respectively. For total flavonoids, the highest content was obtained with the methanolic extract of P. pentandrus, with 3.12 ± 0.01 mg QE/100 mg of extract. These findings justify the uses of these plants in traditional medicine for the treatment of infectious diseases such as diarrhea and can be used as starting points for the development of phytodrugs against infectious diarrhea.

Optimization of Natural Dye Extraction from Coconut Husk

This work aims to optimize the extraction of natural dye from coconut husks for use in the textile industry. The optimization process was carried out using the One-Factor-at-a-Time (OFAT) approach. Before optimization, different parts of the coconut husk i.e., endocarp, exocarp, mesocarp, and a mixture of mesocarp and exocarp were screened for their potential to be used as dye. It was found that the combination of mesocarp and exocarp has the highest potential in terms of physicochemical and phytochemical properties as it resulted in the best yield of extract, pH and colour intensity, and the highest Total Flavonoid Content, Total Phenolic Content, and Total Tannin Content. The optimal parameters to extract dye from coconut husk (combination of mesocarp and exocarps) are particle size of 2 mm, sample to solvent ratio of 1:100, extraction time of 60 min, and a temperature of 100 °C. The dye extract was tested for use on cotton fabric. It was found that regardless of the mordanting method, acetic acid is the best mordant to use. It resulted in a brighter and more intense colour than alum. The usage of acetic acid as the mordant combined with the pre-mordant method was found to be the best for colour fixation of the natural dye from coconut husk on cotton fabric.