Total Soluble Phenolic Content Research Articles

Physiological response and proteomic profiling of biochar-induced tomato resistance to bacterial wilt

Biochar has been shown to enhance plant resistance against biotic stress, yet the underlying mechanisms remain unclear. In this study, influence of wheat straw biochar on disease development, plant physiology, and proteomic expression in tomato plants infected with Ralstonia solanacearum were evaluated. The results indicated that biochar application significantly improved plant growth, reduced the severity of disease, and decreased the colonization of R. solanacearum in tomato stems. Physiological analysis revealed that biochar treatment significantly increased peroxidase and lipoxygenase activities, as well as the content of total soluble phenolics and lignin-like phenolic polymers in tomato leaves under pathogen infection. Moreover, proteomic analysis revealed that biochar treatment upregulated the expression of genes involved in lignin synthesis, anion channel and sulfur metabolism, and downregulated the expression of genes involved in microtubule and cytoskeleton organization, as well as UDP-glucose metabolism.In conclusion, our study demonstrates that biochar improves the resistance of tomato plants to bacterial wilt through enhancing lignin deposition, limiting pathogen movement, scavenging reactive oxygen species (ROS), and reducing the stress response that triggers early-stage plant defense.

Improvement of shelf-life quality of ivy gourd (Coccinia grandis L. Voigt) using an exogenous coating of mannitol and sorbitol

Mannitol and sorbitol are considered as suitable osmolytes. In light of this, the two reagents were applied (100 µM to 400 µM individually and combinations of 100 µM + 100 µM to 400 µM + 400 µM) by dipping the ivy gourd fruits in them. The physiological along with physical parameters were assessed with and without (considered to be controlled) dipping in these reagents. The results of the present study reveal that the rate of physiological and physical changes in ivy gourds during storage is influenced by the amount of exogenous mannitol-sorbitol used. In addition to significantly lowering weight, pH, and titrable acidity, the mannitol-sorbitol coating of ivy gourds increases phosphomolybdate activity, total soluble sugar, phenolic content, and pectate lyase activity. It has enzymes that fight free radicals, including peroxidase and catalase, whose activity decreases in stressful situations. In comparison to the other treatments and the untreated ivy gourds, the 300 µM mannitol treatment offered the highest amount of protection. Hence, ivy gourds' postharvest quality can be effectively extended by mannitol and sorbitol for up to 10 days without significantly affecting their phytochemical and physiological qualities.

Применение ферментов при выработке фруктового сока: обзор

Enzymatic treatment is a common method of fruit juice production that facilitates juice extraction from plant cells. The choice of enzyme depends on the fruit composition. Pectinase and cellulase are the most popular enzymes while amylase remains less wide-spread. For some raw materials, enzymatic procedures are more efficient than mechanical comminution or thermal processing. The fruit juice industry uses enzymes for streamlining. Enzymes maximize juice extraction from raw materials and improve such processes as pressing, solid settling, and solid removal. Juices that underwent enzymatic treatment are clear and, as a result, more aesthetically appealing to consumers.
 The review covered the most recent and influential publications on the enzyme treatment of fruit juices (2000–2021). The list of enzymes included pectinase, cellulase, and amylase. The research included the factors that affect the juice fermentation process, i.e., hydromodule, enzyme concentration, incubation time, temperature, and enzyme combination. The methods included data extraction, data analysis, and data compilation, as well as literature search and screening.
 The review focuses on the effects that individual parameters have on specific responses, e.g., yield, viscosity, total soluble solids, acidity, turbidity, clarity, pigment concentration, phenolic content, color, and solids. A greater enzyme concentration, incubation time, and temperature decrease the viscosity of juice and turbidity but cause color changes. If used in different combinations and at different concentrations, enzymes boost the production of bael pulp, banana, sapodilla, durian, pawpaw, grape, white pitaya, and water melon juices. A longer incubation period improves the production of bael pulp, citron, date, and pawpaw juices. However, higher incubation temperatures seem to have no positive effect on the juice yield. Cellulases, pectinases, amylases, and their combinations are able to produce more fruit juice of higher quality with a more favorable time-temperature combination of incubation.
 The optimal enzyme concentration, incubation time, and temperature can increase the juice yield. Therefore, enzymatic treatment is an effective method that ensures favorable properties of the finished product.

Glutathione primed seed improved lead-stress tolerance in Brassica rapa L. through modulation of physio-biochemical attributes and nutrient uptake

Heavy metal toxicity is a major environmental issue that affects all life forms, including plants. The accumulation of lead (Pb) in agricultural soils is a significant contributor to reduced crop yields, and it poses serious health risks to people who consume lead-contaminated agricultural products. The current study was undertaken to investigate the beneficial effects of glutathione (GSH) on the amelioration of stress induced by Pb (300 mg kg−1 Pb) in Brassica rapa L. (turnip). For this purpose, B. rapa seeds primed with 25, 50, and 75 µmol L−1. The root and shoot length, seedling biomass, and leaf area, was reduced under Pb stress. Lead toxicity inhibited the net photosynthetic rate (31.36%), total chlorophyll content (74.54%) of B. rapa plants in comparison to control. Lead-stressed plants additionally exhibited changes in proline levels, as well as lower levels of total soluble protein and phenolic content. Nevertheless, seed priming with GSH resulted in higher concentrations of the nutritional content (Mg+2, Zn+2, Na+, K+) that increased Pb stress tolerance. The GSH2 treated seed enhanced the photosynthetic rate (46.34%), stomatal conductance (80.55%), and transpiration rate (53.125%) over Pb stress. Furthermore, GSH2 enhanced total soluble proteins (37.75%), phenolic content (58.38%), and DPPH (1.5fold) of turnip plant over control seedlings. According to our research, GSH2 primed B. rapa seed demonstrated a reduction in Pb toxicity, which could be used to help seedling establishment in soils contaminated with Pb.

Improving the nutritional quality and bio-ingredients of stored white mushrooms using gamma irradiation and essential oils fumigation

Abstract Mushrooms are highly perishable in nature and deteriorate within a few days after harvesting due to their high respiration rate and delicate epidermal structure. Consequently, the shelf-life of freshly harvested mushroom is limited to 1–3 days at ambient condition. Hence, the current investigation was carried out to study γ-irradiation effects (1.5 and 2.0 kGy) and essential oils (EOs) fumigation including geranium (60 and 80 μL/L) and lemongrass (40 and 60 μL/L) on nutritional quality (Vitamins C and D2) as well as bio-ingredients such as total soluble proteins, phenolic and flavonoids contents, antioxidant activity were determined as an origin of potential natural antioxidant plus the profile of phenols and flavonoids identified by HPLC. As well as activities of some enzymes (PPO, SOD, PAL, and APX) of Agaricus bisporus mushroom at 4 °C during storage time for twelve days. The findings showed that there was a reduction in the contents of Vit. C and vitamin D2 in all mushroom samples during storage, where the essential oil treatment especially 60 μL/L of geranium and 40 μL/L of lemongrass gave the least decrease (3.42 and 3.28 mg/100 g FW, respectively) of ascorbic acid content compared to the other treatments while the irradiated samples (1.5, and 2.0 kGy) gave the lowest decrease of vitamin D2 (106.30 and 114.40 mg/kg DW, respectively) at the end of storage time. The content of the bio-ingredients content was affected by the storage periods, and the samples treated with oil fumigation gave the best content and the same trend happened with the antioxidant activity. The enzymes activity increased by the storage period, especially after 4 days of storage, and then the activity decreased after that. Quantification of phenolic and flavonoid compounds affected by storage periods in all treatments and the EO-treated mushrooms gave the best amount of them. Thus, samples of mushrooms treated with oil fumigation especially 60 μL/L of geranium and 40 μL/L of lemongrass can successfully increase the nutritional value plus maintain the value of the mushrooms during storage time.

Corn distiller’s dried grains with solubles as a target for fermentation to improve bioactive functionality for animal feed and as a source for a novel microorganism with antibacterial activity

Plant-based bioenergy by-products such as corn distillers’ dried grains with solubles (DDGS) are widely utilized as animal feed sources and feed ingredients due to their balanced nutritional profile and animal health protective functional qualities. Bioprocessing of this bioenergy by-product using beneficial lactic acid bacteria (LAB)-based fermentation strategy to improve animal-health targeted functional qualities has wider relevance for animal feed applications. In this study, liquid extracts of corn DDGS were fermented with Lactiplantibacillus plantarum and Lactobacillus helveticus. The unfermented and fermented extracts were then analyzed (at 0, 24, 48 and 72-h) for their total soluble phenolic content (TSP), phenolic profile, antioxidant activity via ABTS and DPPH radical scavenging activity, and antimicrobial activity against the gut pathogen Helicobacter pylori using in vitro assay models. Statistical differences in antioxidant activity and phenolic content were observed among the unfermented and fermented extracts. The major phenolic compounds detected in corn DDGS were gallic, dihydroxybenzoic, p-coumaric, caffeic and ferulic acid, and catechin. Antimicrobial activity against H. pylori was observed for the unfermented extracts and the antimicrobial activity was attributed to the growth of a corn DDGS-endemic culture. The culture was isolated, sequenced, and identified as Bacillus amyloliquefaciens. Results of this study indicated that processing strategies of by-products such as LAB- based fermentation of corn DDGS could affect its bioactive-linked functional qualities due to microbial interaction with the phytochemicals. Furthermore, plant by-products can serve as novel sources of beneficial microflora that have relevance in wider agriculture, food safety, and therapeutic applications.

Quality Traits and Nutritional Components of Cherry Tomato in Relation to the Harvesting Period, Storage Duration and Fruit Position in the Truss.

It is well known that the harvesting period and the storage duration have a significant effect on the quality characteristics of cherry tomato fruits. On the other hand, the effect of the fruit position in the truss has not been studied, as well as the relative contribution of each one of these factors on fruit quality. For this purpose, cherry tomato (Genio F1) whole trusses were harvested at the fruit red ripe stage during three periods. At each harvesting period, the first four (at the base of the truss) and the last four (at the top) fruits from each truss that was previously trimmed to 10 fruits, were stored at 12 °C for 0, 4 and 10 days. At the end of each storage duration, the external color, firmness, antioxidant capacity, pH and titratable acidity, as well as dry matter, soluble solid, total soluble phenol, lycopene, total carotenoid and β-carotene content, were determined. Analysis of variance (ANOVA) indicated that the harvesting period had the most significant effect on skin color parameters L * and C * and β-carotene, as well as on antioxidant capacity, total soluble phenols, dry matter and total soluble solids, while it also had an appreciable effect on titratable acidity. The storage duration had a dominant effect on firmness, total carotenoids and lycopene, while it had an appreciable effect on skin color parameter L * as well. On the other hand, the fruit position in the truss exerted an exclusive effect on ho and a */b * ratio skin color parameters and pH and an appreciable effect on titratable acidity.

The Effects of Fixation Methods on the Composition and Activity of Sea Buckthorn (Hippophae rhamnoides L.) Leaf Tea

Fixation is the key step to improve the quality of sea buckthorn leaf tea. Composition and activity are important indexes to evaluate the quality of sea buckthorn leaf tea. Comparing the effects of fixation methods on components and activities provides a theoretical basis for the contemporary, controllable, and continuous production of sea buckthorn leaf tea. The effects of six different fixed methods, pan-firing fixed (PF), steaming fixed (SF), boiling water fixed (BF), hot air fixed (HF), microwave fixed (MWF), and infrared fixed (IRF) for sea buckthorn leaf tea in terms of α-glucosidase inhibitory activity, lipase inhibitory ability, and the antioxidant capacity were studied. The total flavonoids (TF) content, total soluble phenolics (TP) content, water-soluble carbohydrate (WSC) content, the inhibitory activity of α-glucosidase, lipase inhibitory ability, and the antioxidant capacity of fixed sea buckthorn leaf tea were significantly higher ( p ≤ 0.05 ) compared with sea buckthorn leaf. IRF and MWF samples had higher ( p ≤ 0.05 ) contents of TF (92.48 mg RE/g and 79.20 mg RE/g), TP (115.37 mg GA/g and 135.18 mg GA/g) and WSC (4.24% and 4.39%). The DPPH radical scavenging activity of the SF sample was the strongest one, followed by the MWF sample and IRF sample ( p ≤ 0.05 ). The hydroxyl radical scavenging ability and reducing power of IRF were the strongest one, followed by the MWF sample ( p ≤ 0.05 ). The IRF sample had the strongest α-glucosidase inhibitory activity ( p ≤ 0.05 ), and the MWF sample had the strongest lipase inhibitory ability while samples contained the same amount of total polyphenols ( p ≤ 0.05 ). Principal component analysis results showed that the IRF sample, MWF sample, and SF sample had higher comprehensive principal component values. MWF takes less time than IRF, which operated at 2,450 MHz (full power of 700 W) for 2 min. Therefore, MWF was the most suitable fixation method for sea buckthorn leaf tea. Practical Applications. Leaf tea is the main product of sea buckthorn leaf. However, at present, the quality of sea buckthorn leaf tea in the market is uneven, the processing methods are diverse, and there is no certain quality standard. This paper provides some data support and theoretical support for the production, processing, and purchase of sea buckthorn leaf tea.

Drying Characteristics and Quality Analysis of Medicinal Herbs Dried by an Indirect Solar Dryer.

Considering the solar radiation status in Oman, a low-cost, indirect, stand-alone, forced-convective solar dryer was developed to dry medicinal herbs, which are sensitive to direct sun. The hot air flow was obtained using a solar-panel-powered blower and air passing through a black-body solar collector. This drying process could extend the shelf life of herbs while preserving their medicinal and nutritional (physicochemical) properties and adhering to food safety and hygiene practices. This study investigated the benefits of an indirect solar drying technique on the retention of quality attributes of mint and basil used in medicinal applications. Herbs used during drying could be subjected to changes in their physicochemical properties such as color, water activity (Aw), total soluble solids (TSS), phenol content, antioxidant capacity, and moisture content (MC), and, thus, results were compared with fresh herb samples. The dryer chamber-maintained temperature and relative humidity regimes of 30-50 °C and 21-95% and the expected final moisture content (wet basis) was 10%. The dryer showed improved physicochemical quality parameters and the retention of green color with parameter ranges of Aw 0.44-0.63, phenol content (increase) 1705-8994 mg/100 g DM, and antioxidant capacity (increase) 0.61-0.67 µmol/g DM, respectively. This study showed the ability of developed solar dryers to preserve the physicochemical properties of medicinal herbs during drying and can extend to other food products.

Assessment of nitrogen-fixing endophytic and mineral solubilizing rhizospheric bacteria as multifunctional microbial consortium for growth promotion of wheat and wild wheat relative Aegilops kotschyi

Microbes play crucial functions in maintaining the health and growth of the plants directly or indirectly by supplying nutrients. These microbes could be used as biofertilizers for the enhancement of soil health and growth of crops. In preset investigation, potential microbes from endophytic and rhizospheric region of Aegilops kotschyi growing in green slopes of Shivaliks, Himachal Pradesh were sorted out and screened for plant growth promoting attributes including phosphorus and potassium solubilization. The potential bacterial strains were identified through 16S rRNA gene sequencing and developed as microbial consortium for the plant growth of wheat and wild wheat relative Aegilops kotschyi. A total 125 isolates of bacteria were sorted out and among them 36 were found as P-solubilizers and 19 showed K-solubilization attribute and two highly potential bacterial strain were identified as Bacillus tropicus EU-ARP-44 (P-solubilizer; 270.5 ± 0.00 mg L−1) and B. megaterium EU-ARK-23 (K-Solubilizer; 51.3 ± 1.7 mg mL−1). The microbial consortium of Rahnella sp. strain EU-A3SNfb (N-fixer; MN294545), B. tropicus EU-ARP-44 (P-solubilizer) and B. megaterium EU-ARK-23 (K-solubilizer) evaluation in Aegilops kotschyi and wheat crop resulted in the enhancement of growth as well as physiological parameter including shoot/root length, fresh/dry weight and chlorophyll, carotenoid, total soluble sugar content, phenolic and flavonoid content as compared to un-inoculated control. Microbial consortium consisting of potential plant growth promoting (PGP) bacterial strains could be used as biofertilizer and bioinoculants in cereals crop growing in hilly region.

Zinc oxide nanoparticles (ZnONPs) influenced seed development, grain quality, and remobilization by affecting the transcription of microRNA 171 (miR171), miR156, NAM, and SUT genes in wheat (Triticum aestivum): a biological advantage and risk assessment study.

Limited studies have been conducted on the role of microRNAs (miRs) and transcription factors in regulating plant cell responses to nanoparticles. This study attempted to address whether the foliar application of zinc oxide nanoparticles (ZnONPs; 0, 10, 25, and 50 mgL-1) can affect miRs, gene expression, and wheat grain quality. The seedlings were sprayed with ZnONPs (0, 10, 25, and 50 mgL-1) or bulk counterpart (BZnO) five times at 72h intervals. The application of ZnONPs at 10 mgL-1 increased the number of spikelets and seed weight, while the nano-supplement at 50 mgL-1 was accompanied by severe restriction on developing spikes and grains. ZnONPs, in a dose-dependent manner, transcriptionally influenced miR156 and miR171. The expression of miR171 showed a similar trend to that of miR156. The ZnONPs at optimum concentration upregulated the NAM transcription factor and sucrose transporter (SUT) at transcriptional levels. However, the transcription of bothNAMandSUTgenes displayed a downward trend in response to the toxic dose of ZnONPs (50 mgL-1). Utilization of ZnONPs increased proline and total soluble phenolic content. Monitoring the accumulation of carbohydrates, including fructan, glucose, fructose, and sucrose, revealed that ZnONPs at 10 mgL-1 modified the source/sink communication and nutrient remobilization. The molecular and physiological data revealed that the expression of miR156 and miR171 is tightly linked to seed grain development, remobilization of carbohydrates, and genes involved in nutrient transportation. This study establishes a novel strategy for obtaining higher yields in crops. This biological risk assessment investigation also displays the potential hazard of applying ZnONPs at the flowering developmental phase.

BIOCHEMICAL CHANGES IN LEAVES OF RESISTANT AND SUSCEPTIBLE URDBEAN GENOTYPES INFECTED WITH URDBEAN LEAF CRINKLE VIRUS (ULCV)

Urdbean leaf crinkle virus (ULCV) is a widespread and most destructive virus that causes leaf crinkle disease in black gram. Virus infection causes alteration in physiological and biochemical processes within the plant. In present study, effect of viral infection was investigated on chlorophyll content, total soluble sugar, total protein, phenolic contents, sodium dismutase (SOD) and nitrate reductase activities in the leaves of susceptible and resistant genotypes of urdbean at 30 days after virus inoculation. Disease symptoms were mild (6.7% to 10%) in resistant genotypes and severe (36% to 43%) in susceptible genotypes. ULCV infection resulted in a significant decrease in chlorophyll content in infected plants of all genotypes over control plants; however, magnitude of reduction was more in susceptible genotypes (41%) as compared to the resistant genotypes (14.35%). The total sugar and protein contents were significantly higher in the inoculated susceptible (210.26 % and 68.67%, respectively) as compared to the resistant genotypes (183.87% and 25.89%, respectively). Also, the total phenolic content was significantly higher in inoculated resistant genotypes (83.91%) over the susceptible genotypes (25.89%). There was significant decrease in the activity of superoxide dismutase enzyme in resistant genotypes as compared to susceptible inoculated genotypes. Similarly, a reduction in nitrate reductase activity was more in inoculated plants of resistant genotypes. Our results indicate that post-infection significant increases in total sugars and proteins in susceptible genotypes and phenols in resistant genotypes may have a role in disease resistance and development. Changes in enzymatic activities in resistant and susceptible genotypes might also have some role in resistance against ULCV.

Defense Response to Fusarium Infection in Winter Wheat Varieties, Varying in FHB Susceptibility, Grown under Different Nitrogen Levels

Fusarium head blight and inadequate nitrogen fertilization can cause numerous biochemical changes in wheat. The main aim of this study was to determine the effects of Fusarium inoculation and a broader range of different nitrogen fertilization on the defense response in the spikes of four wheat varieties, varying in FHB susceptibility. Total soluble phenolics content, activities of enzymes involved in phenol metabolism (PAL and PPO), and total antioxidant capacity were determined as indicators of defense response. In both growing seasons, Fusarium inoculation altered PHE content in wheat spikes, indicating involvement of PHE in the defense response to Fusarium attack. Increased PHE content in the partially resistant varieties (Apache and Graindor) indicates involvement of PHE in the defense response and better disease tolerance in the more resistant varieties. Breeding wheat varieties with enhanced PHE synthesis could be a promising strategy to control FHB. To the best of our knowledge, this is the first study that emphasizes the effects of Fusarium infection and a broader range of different nitrogen fertilization on PHE and enzymes involved in PHE metabolism. In addition, this is the first study using the FRAP method to determine the antioxidant capacity of wheat tissues under the influence of Fusarium infection and different nitrogen fertilization.

Chitosan augments bioactive properties and drought resilience in drought-induced red kidney beans

Application of 0.25% chitosan during seed priming or its foliage spray on Phaseolus vulgaris L. grown under restricted irrigations was assessed for its role in the enhancement of bioactive properties like antioxidant potential of dry seed. Seven hydroxycinnamic acid and eleven hydroxybenzoic acid derivatives were identified and quantified in seeds collected from treated plants using LC-MS/MS. The major phenolics in all the treatments were in the order; ferulic acid > p-coumaric acid > o-coumaric acid > gallic acid and salicylic acid. However, the concentrations of each differed significantly at p < 0.01, with ferulic acid (324.304 µg/g) in red kidney bean seed grown under 30% irrigation with primed seed as the most predominant phenolic. Phenolics reported are representative of the phenylpropanoid pathway having a defined role in combating abiotic stress. The DPPH free radical scavenging activity, metal chelation, and reducing power antioxidant activities of the crude extract of seeds were investigated and found to elevate with increase in imposing more drought stress. Total soluble phenol content was found to have a strong correlation with the antioxidant activities with DPPH radical scavenging activity (-0.88), reducing power activity (0.87) and chelating power activity (-0.85). Principal components PC1 and PC2 accounted for almost 64.40% as the principal contributors in augmenting the bioactive properties and drought resilience. Overall, our findings indicate that 0.25% chitosan as seed and foliar spray augments bioactive properties and drought resilience in drought induced red kidney beans.

Production and Shelf-Life Study of Probiotic Caja (Spondias mombin L.) Pulp Using Bifidobacterium animalis ssp. Lactis B94.

The highly nutritional caja fruit (Spondias mombin L.) is an accessible source of vitamins and antioxidants that are indispensable for the human diet. The objective of the present work was to study the production of a probiotic caja pulp using Bifidobacterium animalis ssp. lactis B94. Firstly, a kinetic study was performed on the fermentation of the caja pulp with Bifidobacterium animalis ssp. lactis B94 to determine the optimum conditions of the process. Growth kinetics revealed that the ideal time for ending the fermentation would be at 22 h because it corresponds to the end of the exponential phase. Both the whole pulp and the probiotic pulp were characterized for pH, acidity, total soluble solids, water content, phenolic content, reducing carbohydrates, ascorbic acid, and total carotenoids. Physicochemical characterization revealed similar results between the whole and the probiotic pulp. The stability test demonstrated that the probiotic pulp is stable and preserved the probiotic attributes of the final product. In conclusion, our results reveal that caja pulp can be considered a favorable medium for the Bifidobacterium animalis ssp. lactis B94 growth and consequently can be explored biotechnologically for new food products.

Effect of mango kernel seed starch-based active edible coating functionalized with lemongrass essential oil on the shelf-life of guava fruit

In the present investigation, the mango kernel seed starch-based edible coating was developed combined with different concentrations of lemongrass essential oil (EO). The effects of different edible coating formulations, such as T1 (2% mango kernel seed starch); T2 (2% mango kernel seed starch + 0.25% lemongrass essential oil); T3 (2% mango kernel seed starch + 0.5% lemongrass essential oil); and T4 (2% mango kernel seed starch + 1% lemongrass essential oil), were investigated for physiological, physiochemical, and microbiological properties of fresh guava fruit stored at 23 ± 2°C and a relative humidity of 85 ± 5% for up to 9 days. Results of the present study revealed that the mango kernel seed starch edible coating combined with lemongrass essential oil was effective in enhancing the storage life of guava fruit by maintaining their quality attributes. Treatment of mango kernel seed starch with 0.5% lemongrass essential oil (T3) was the most significant (P &lt; 0.05) treatment to reduce physiological loss in weight and oxidation rate while maintaining the physiochemical characteristics, such as acidity, total soluble solids, textural property, phenolic contents, and sensory profiles of stored guava fruit samples, followed by T4 treatment. The microbial load of yeast and mold was also reduced by T3 and T4 treatments. All coating treatments were potent to enhance the storage life of guava fruit with improving post-harvest quality attributes for up to 9 days of storage. However, retention of higher quality of fruit was observed with T3 treatment.

Effect of mango kernel seed starch-based active edible coating functionalized with lemongrass essential oil on the shelf-life of guava fruit

In the present investigation, the mango kernel seed starch-based edible coating was developed combined with different concentrations of lemongrass essential oil (EO). The effects of different edible coating formulations, such as T1 (2% mango kernel seed starch); T2 (2% mango kernel seed starch + 0.25% lemongrass essential oil); T3 (2% mango kernel seed starch + 0.5% lemongrass essential oil); and T4 (2% mango kernel seed starch + 1% lemongrass essential oil), were investigated for physiological, physiochemical, and microbiological properties of fresh guava fruit stored at 23 ± 2°C and a relative humidity of 85 ± 5% for up to 9 days. Results of the present study revealed that the mango kernel seed starch edible coating combined with lemongrass essential oil was effective in enhancing the storage life of guava fruit by maintaining their quality attributes. Treatment of mango kernel seed starch with 0.5% lemongrass essential oil (T3) was the most significant (P &lt; 0.05) treatment to reduce physiological loss in weight and oxidation rate while maintaining the physiochemical characteristics, such as acidity, total soluble solids, textural property, phenolic contents, and sensory profiles of stored guava fruit samples, followed by T4 treatment. The microbial load of yeast and mold was also reduced by T3 and T4 treatments. All coating treatments were potent to enhance the storage life of guava fruit with improving post-harvest quality attributes for up to 9 days of storage. However, retention of higher quality of fruit was observed with T3 treatment.

Evolution of Volatile Aroma Compounds and Amino Acids in Cabernet Gernischt Grape Berries (Vitis vinifera L.): Comparison of Different Training Systems for Mechanical Soil Burial.

Soil burial is a survival strategy for grapevines that can safely overwinter in north-western regions of China. A suitable training system was beneficial for soil burial to provide winter protection. Moreover, the training system can also significantly affect fruit quality during the development of grape berries, such as primary and secondary metabolites. In this study, four-year-old Cabernet Gernischt grapevines were used as experimental material and exposed to four training systems, including the Ningxia traditional vertical trunk (control, CK); the gobelet (T1); single guyot (T2); slant trunk with vertical shoot positioning (STVSP) (T3). The results showed that total soluble solid total phenol content was 12.69%, 57% higher under T3 training systems than in the control, and T3 alleviated the canopy density, leading to improving the leaf photosynthetic efficiency gas chromatography-mass spectrometry (GC-MS) assay used to detect the aroma compounds. The results indicated that the T3 training system enhanced the accumulation of alcohols, carbonyl compounds, C6/C9 and esters, which account for the largest proportion of volatile compounds, and the qRT-PCR reveals that VvEcar, Vvter, VvCCD1, and VvLis were raised under T3 at the transcriptional level. Moreover, T3 contributes to most free amino acid synthesis. Additionally, the PCA reveals the correlation of free amino acids under four training systems, which reflected the mostly amino acid related to T3, and thus, we could speculate that T3 enhances the overall aroma. These results may lead to new strategies to select a new, short trunk training system to achieve mechanized buried soil, to prevent cold and produce high-quality wine in this area.

Scouting for Multiple Disease Resistance in Urdbean

Background: Dry root rot (DRR), yellow mosaic disease (YMD) and stem necrosis diseases are inflicting the economic losses in urdbean. Information on the interrelation of morphological and biochemical parameters of the urdbean cultivars with the disease resistance is scarce. Methods: Thirty nine cultivars of urdbean were screened in the field for their resistance to DRR, YMD and stem necrosis diseases during Rabi, 2019-2020. The incidence of the diseases is compared with the morphological characters viz., trichome density, leaf thickness, root thickness and biochemical composition viz., total free amino acids, total soluble sugars and phenol content of cultivars. Result: Cultivar GBG 103 was found resistant to DRR and YMD with the maximum amount of trichome density. No cultivar was found resistant to stem necrosis under field conditions. Correlation analysis revealed the positive correlation of phenol content with the DRR incidence; trichome density and total soluble sugars with YMD incidence. Albeit the leaf thickness and total free amino acid content of the cultivars were negatively correlated with the incidence of the DRR, YMD and stem necrosis diseases.

Evaluation of Eucalyptus Clones for Susceptibility to The Gall Wasp Letocybe invasa Fisher and La Salle

This study investigates the variation among Eucalyptus clones for incidence of gall wasp (Letocybe invasa) and relative changes in biochemical parameters. The three years, and replicated clonal trial involved 14 clones with monthly observations made on the gall wasp incidence. Significant variation was found among the clones, with maximum incidence being in F-316 and relatively the least incidence in C-413, PE-11, PE-14 and C-72. Gall wasp incidence gradually increased from January to April and declined thereafter. The total soluble sugars and phenol content in the infested leaves were found to increase by 49.2 and 22.8% respectively, whereas the protein content decreases (8.8% decrease).