Total Solids Recovery Research Articles

Artificial Intelligence-Powered Optimization and Milk Permeate Upcycling for Innovative Sesame Milk with Enhanced Probiotic Viability and Sensory Appeal.

Consumer demand for plant-based alternatives drives innovation in nondairy beverages. This study explores the development of a novel sesame milk with enhanced functionality using an artificial neural network (ANN) and milk permeate integration. An ANN model effectively optimized water-based sesame milk (WSM) extraction, maximizing total solids (T.S.) recovery. The ANN model's predicted T.S. yield (99.65%) closely matched the actual value (95.18%), demonstrating its potential for optimizing high-yield production. Furthermore, milk permeate was incorporated (5:1 ratio) to create permeate-based sesame milk (PSM), which supported the growth of lactic acid bacteria, suggesting its potential as a growth medium for future probiotic applications. PSM also displayed superior nutritional value and sensory characteristics compared to WSM. These findings highlight the promise of ANN-powered optimization and milk permeate integration for creating innovative sesame milk alternatives with enhanced probiotic viability and sensory appeal. Future research should focus on ANN optimization of alternative-based-plant milk, including permeate-based sesame milk production, the health benefits of LAB fermentation, and consumer preferences for flavors and textures. Optimizing fermentation and LAB selection remain key for commercial success.

One-Step Extraction of Olive Phenols from Aqueous Solution Using β-Cyclodextrin in the Solid State, a Simple Eco-Friendly Method Providing Photochemical Stability to the Extracts.

The extraction of phenolic compounds from olive mill wastes is important, not only to avoid environmental damages, but also because of the intrinsic value of those biophenols, well-known for their high antioxidant potential and health benefits. This study focuses on tyrosol (Tyr) and hydroxytyrosol (HT), two of the main phenolic compounds found in olive mill wastes. A new, simple, and eco-friendly extraction process for the removal of phenolic compounds from aqueous solutions using native β-cyclodextrin (β-CD) in the solid state has been developed. Several β-CD/biophenol molar ratios and biophenol concentrations were investigated, in order to maintain β-CD mostly in the solid state while optimizing the extraction yield and the loading capacity of the sorbent. The extraction efficiencies of Tyr and HT were up to 61%, with a total solid recovery higher than 90% using an initial concentration of 100 mM biophenol and 10 molar equivalents of β-CD. The photochemical stability of the complexes thus obtained was estimated from ∆E*ab curve vs. illumination time. The results obtained showed that the phenols encapsulated into solid β-CD are protected against photodegradation. The powder obtained could be directly developed as a safe-grade food supplement. This simple eco-friendly process could be used for extracting valuable biophenols from olive mill wastewater.

Adapting Santiago Method to Determine Appropriate and Resource Efficient Sanitation Systems for an Urban Settlement in Lima Peru

The pre-selection of locally appropriate sanitation technologies and systems is crucial for strategic sanitation planning as any decision is only as good as the options presented. One approach that allows us to systematically consider the local conditions and a diverse range of conventional and novel technologies and systems is the Santiago method. In this paper, we discuss whether the Santiago method can be applied to the case of Latin America and what we would gain from this application. We do so by expanding the Santiago technology library with technologies that have been shown to be promising in metropolitan areas of Latin America, such as condominial sewer, container-based sanitation, and activated sludge. We then apply Santiago to the semi-informal settlement Quebrada Verde (QV) in Lima, Peru. Using Santiago, we were able to generate 265,185 sanitation system options from 42 technologies and 18 appropriateness criteria. A set of 17 appropriate and divers are then selected. The diversity is defined by 17 system templates. To further evaluate these 17 systems, resource recovery and loss potentials are quantified. Higher nutrients (nitrogen and phosphorus) and total solids recovery are observed for systems that combine urine diversion and biofuel production. The case of QV shows that the Santiago method is applicable in the Latin American context.

Comparison of Modern Drinking Water Network Maintenance Methods: Evaluation of Removed Deposits in the Form of Total Suspended Solids (TSS).

Water pipe sediment removal should be implemented as an integral part of water mains maintenance in order to steadily supply consumers with drinking water of high quality. Considering the number of different water pipe sediment removal methods, the article aims to evaluate the currently used methods to remove water pipe sediment from the pipes of the drinking water distribution system. The evaluation compares the implementation requirements of each method as well as the quality and the quantity of the removed products. The tested methods were unidirectional flushing, Comprex®, and Ice Pigging®. The results of the comparison are expressed in terms of total suspended solids (TSS) recovery, metals mass concentration and water consumption. Since contamination can settle along the entire surface of the pipeline, it is most appropriate to recalculate the results per unit area of the pipeline. The results point at the following efficiency the Comprex® method was the most efficient in removing TSS, Ice Pigging® was the next and unidirectional flushing removed a negligible amount of TSS compared to the other two methods. The absolute recovery of TSS was 0.12–3.01 g·m−2 in unidirectional flushing of plastic pipes, 1.58–8.54 g·m−2 in unidirectional flushing of metal pipes, 4.36–47.53 g·m−2 in Ice Pigging®, and 5.19–69.23 g·m−2 in Comprex®. The composition of the sediment was strongly influenced by particle origin: Pipe material affected the crystalline phase of the sediment and the water source and the age of the pipe affected the amorphous phase of the sediment. Therefore, it was found that evaluation of efficiency based on the amount of TSS removed is only suitable for sites that meet the same conditions as pipe material, water source and ideally the pipe age. It has further been found that the Comprex® method can be advantageously used in real conditions to clean pipes with insufficient hydraulic conditions (such as with a high level of incrustation), as the cleaning has low water flow velocity requirements.

Production of Pre Ripened Provolone Cheese and Quality Characterization Using Blends of Whole Milk

Cheese is a product made from the curd obtained from milk by coagulating the casein with the help of rennet in the presence of lactic acid produced by added starter culture. The study was aimed to evaluate the yield and quality of pre ripened provolone cheese prepared from different blends of cow, doe, ewe and camel whole milk. The sources of milk were from Hawassa town, Langano and Kofele areas of Oromiya region. Pre ripened provolone cheeses were made from blends contained ratio between 60-80%, 10-30%, 10-30% and 0-20% for cow, doe, ewe and camel milk, respectively using standard procedure. The whole milk and its pre ripened provolone cheeses were subjected to physicochemical analyses. The physicochemical analyses, selected minerals, bioactive compounds, microbial quality and consumer acceptability of the pre ripened provolone cheese were analyzed. The blending proportion of different milk had significant (P<0.05) effect on the physiochemical property, mineral, bioactive and sensory quality of pre ripened provolone cheese. The physiochemical property of whole milk for manufacture of pre ripened provolone cheeses were in the range between 10.56 to 15.08% for total solids, 3.45 to 5.20% for fat and 3 to 4.19% for crude protein. The chemical composition of pre ripened provolone cheese prepared from different blended milk ranged from 47.32- 67.05% for total solids, 24.26 – 36.81% for fat, 17.78 – 26.30% for crude protein, 1.09 – 3.49% for total ash, and 0.75 – 2.98% for lactose; ascorbic acid (0.49 to 3.08 mg/kg) and total polyphenols (1.00 to 17.50 (mg GAE/g). The fat, protein and total solids recovery of pre ripened provolone cheeses ranged from 64.87% to 95.39%, 54.58% to 84.67% and 41.35% to 59.92% respectively. The yield of pre ripened provolone cheese ranged from 9.22% to 13.47%. Total bacteria count was found to be the predominant micro flora of pre ripened provolone cheeses and reached 5.24 cfu/g in the control cheese. The entire consumer acceptability of the pre ripened provolone cheese was in acceptable range. In conclusion, the pre ripened provolone cheese prepared from T12 (60% cow, 10% doe and 30% ewe) milk gave better cheese yield and had auspicious results in nutritional qualities comparable with that of control cheese and other cheese samples. Keywords: Blends of whole milk, cheese yield, pre-ripened provolone cheese, quality characterization DOI: 10.7176/ALST/82-01 Publication date: October 31 st 2020

Effect of Coagulants on the Yield and Quality of Chhana and Rasogolla

A study was carried out to investigate the effect of different coagulants on the recovery of milk solids, yield and sensory quality of Chhana and the resultant Rasogolla. Six type of coagulants comprising of sour whey (0.5%, 1.0% acidity), citric acid (0.5%, 1.0% acidity) and blend of sour whey and citric acid (0.5%, 1.0% acidity) were used to prepare Chhana followed by sweet meat Rasogolla. The nonsignificant yield of chhana was found by the use of different coagulants; however it had a significant influence on the fat and total solids (TS) recoveries ranges from 90.5 to 93.8%; 42 to 44% of milk for chhana, respectively. The highest mean yield, fat recovery and TS recovery were recorded for B type chhana prepared using 1% sour whey as coagulant. Rasogollas prepared using varying coagulants did not have any significant influence on any of the sensory attributes of product studied. Rasogolla prepared from chhana made using 1% sour whey as coagulant had the highest overall sensory score. It is recommended to employ sour whey with 1.0 % of titratable acidity as coagulating agent in the preparation of Rasogolla.

Effects of indirect indicators of udder health on nutrient recovery and cheese yield traits in goat milk

In dairy goats, very little is known about the effect of the 2 most important indirect indicators of udder health [somatic cell count (SCC) and total bacterial count (TBC)] on milk composition and cheese yield, and no information is available regarding the effects of lactose levels, pH, and NaCl content on the recovery of nutrients in the curd, cheese yield traits, and daily cheese yields. Because large differences exist among dairy species, conclusions from the most studied species (i.e., bovine) cannot be drawn for all types of dairy-producing animals. The aims of this study were to quantify, using milk samples from 560 dairy goats, the contemporary effects of a pool of udder health indirect indicators (lactose level, pH, SCC, TBC, and NaCl content) on the recovery of nutrients in the curd (%REC), cheese yield (%CY), and daily cheese yields (dCY). Cheese-making traits were analyzed using a mixed model, with parity, days in milk (DIM), lactose level, pH, SCC, TBC, and NaCl content as fixed effects, and farm, breed, glass tube, and animal as random effects. Results indicated that high levels of milk lactose were associated with reduced total solids recovery in the curd and lower cheese yields, because of the lower milk fat and protein contents in samples rich in lactose. Higher pH correlated with higher recovery of nutrients in the curd and higher cheese yield traits. These results may be explained by the positive correlation between pH and milk fat, protein, and casein in goat milk. High SCC were associated with higher recovery of solids and energy in the curd but lower recovery of protein. The higher cheese yield obtained from milk with high SCC was due to both increased recovery of lactose in the curd and water retention. Bacterial count proved to be the least important factor affecting cheese-making traits, but it decreased daily cheese yields, suggesting that, even if below the legal limits, TBC should be considered in order to monitor flock management and avoid economic losses. The effect of NaCl content on milk composition was linked with lower recovery of all nutrients in the curd during cheese-making. In addition, high milk NaCl content led to reductions in fresh cheese yield and cheese solids. The indirect indicators of the present study significantly affected the cheese-making process. Such information should be considered, to adjust the milk-to-cheese economic value and the milk payment system.

Variation of milk technological properties in sheep milk: Relationships among composition, coagulation and cheese-making traits

The relationships between milk composition, coagulation properties and cheese-making traits in sheep milk were characterised. Ten traits related to milk coagulation (RCTeq, kCF, CFp), cheese yield (%CYCURD, %CYSOLIDS, %CYWATER), and curd nutrients recovery or whey loss (%RECFAT, %RECPROTEIN, %RECSOLIDS, %RECENERGY) were recorded. To obtain a measure of the efficiency in terms of %CY, the ratio between the observed and the theoretical %CY (Ef-%CYCURD, Ef-%CYSOLIDS) was calculated. Sheep milk showed good qualities for coagulation and cheese production; milk lactose appeared to be the component most linked to gelation, curd firming time and water retained in the curd. In the case of milk protein, an opposite relationship with gelation time was observed. Milk fat and protein positively affected total solids recovery and yield inducing higher %CYCURD. Relationships with CFt parameters were limited; curd firming instant rate seems to be the most informative trait to assess the efficiency of the cheese-making process.

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The relationships between milk composition, coagulation properties and cheese-making traits in sheep milk were characterised. Ten traits related to milk coagulation (RCTeq, kCF, CFp), cheese yield (%CYCURD, %CYSOLIDS, %CYWATER), and curd nutrients recovery or whey loss (%RECFAT, %RECPROTEIN, %RECSOLIDS, %RECENERGY) were recorded. To obtain a measure of the efficiency in terms of %CY, the ratio between the observed and the theoretical %CY (Ef-%CYCURD, Ef-%CYSOLIDS) was calculated. Sheep milk showed good qualities for coagulation and cheese production; milk lactose appeared to be the component most linked to gelation, curd firming time and water retained in the curd. In the case of milk protein, an opposite relationship with gelation time was observed. Milk fat and protein positively affected total solids recovery and yield inducing higher %CYCURD. Relationships with CFt parameters were limited; curd firming instant rate seems to be the most informative trait to assess the efficiency of the cheese-making process.

Membrane-based zero-sludge palm oil mill plant

AbstractThe palm oil industry is one of the most important agro-industries for tropical countries because of the unique properties and wide range of uses of palm oil for various end products. In a palm oil extraction process, a large quantity of water is required, of which half the quantity will end up as effluent. This palm oil mill effluent (POME) has an extremely high content of organic matter, which can cause severe pollution of waterways and other environmental problems. Disposal of this highly polluting effluent has become a major problem for the palm oil mills. Therefore, several methods have been proposed either to treat the POME so it could comply with environmental regulation while discharged or to recover water and other valuable components from the effluent. Membrane technology has emerged as a feasible alternative to conventional treatment in vegetable oil processing because of its attractive features such as low energy consumption, reduction in the number of processing steps, high separation efficiency, and improvement of the final product quality. In the case of POME treatment, an integrated membrane-based process promises efficient water recycling and total solid recovery from the effluent, thus eliminating the environmental problem. Recently, a novel concept combining oil–oil extraction and continuous filtration using a superhydrophobic membrane has been proposed to achieve a zero-sludge palm oil mill. In this concept, the huge wastewater effluent generated from the conventional process can be eliminated and the palm oil milling process simplified. Furthermore, the superhydrophobic membrane enables the production of high-purity palm oil. In this paper, we review the prospect of a zero-sludge palm oil mill concept and strategies to achieve the proposed concept. In addition, we also highlight the development of the superhydrophobic membrane and phytonutrient recovery.

A novel oxidative destruction of lignin and enzymatic digestibility of hazelnut shells

This study investigated the effect of sequential oxidative (ozonolysis) pretreatment and enzymatic hydrolysis of hazelnut shells on production of fermentable sugars. Two factors (ozone concentration and pretreatment time), which were determined to be significant by the Box-Behnken response surface methodology, were further maximized for total lignin removal, solid recovery, and total reducing sugar yield via enzymatic hydrolysis of the pretreated biomass using full-factorial design. The ozonolysis was performed by passing 30, 40, and 50 mg/L of ozone gas through a packed bed of ground hazelnut shells for up to 120 min at a flow rate of 0.25 L/min. The highest lignin reduction (20.5%) was obtained in the shells with 30% moisture content subjected to ozone concentration of 50 mg/L for 120 min, which corresponded to a high biomass recovery (94%). The reducing sugar yield also increased from 119.9 mg/g dry untreated biomass to 284.6 mg/g dry untreated biomass for hazelnut shells ozonated at 30 mg/L for 60 min. Thus, these results indicate remarkable benefits of ozonolysis during pretreatment of hazelnut shells.

Effect of Low Temperatures and Residence Times of Pretreatment on Glucan Reactivity of Sodium Hydroxide-Pretreated Rice Straw

Alkaline pretreatment of lignocellulosic biomass is an approach to enhance the susceptibility of the biomass that is subsequently converted into fermentable sugars. The efficacy of the sodium hydroxide pretreatment of rice straw RD41 was evaluated in terms of total solid removal, lignin removal, glucan recovery, and glucan conversion yields. The pretreatment conditions were 50, 60, 70, 80, and 100 °C, and each temperature kept for 1 to 5 h. The effect of pretreatment temperatures was more pronounced than that of the pretreatment times. The elevated temperatures caused higher total solid removal and lignin removal. The highest total solid removal (52.5 to 55.8 %) was found in the pretreatment at 100 °C. At this temperature, the highest lignin removal (~87 %) could be obtained regardless of the residence times of the pretreatment. Most of the glucan (~80 to 100 %) was preserved in the pretreated rice straw. Lower temperatures (50 and 60 °C) favored higher glucan preservation (&gt; 90 %) in the pretreated solids. Glucan conversion of the 3 h pretreatment time samples of each pretreatment temperature revealed that more than 80 % of glucan conversion could be accounted for in samples pretreated at 70 to 100 °C within 24 h of saccharification. The lower temperatures required a prolonged pretreatment time to reach a higher glucan conversion (~90 %), as found in the 50 °C, 5 h pretreated rice straw. The optimal conditions of this simple method are economically feasible, and can be applied to testing the reactivity of herbaceous lignocellulose in future research.

Effect of Feed-Flow Rate in a Solid-Liquid Hydrocyclone Based on Total Solid Recovery Equation

Many studies of hydrocyclones have confirmed that increasing the feed-flow rate results in a higher separation efficiency. The purpose of this study was to investigate the separation efficiency for a 100 mm solid–liquid hydrocyclone with 1 and 2 wt% solid concentrations at feed-flow rates of 2, 3, 4, 5 and 6 m3/hr. The solid concentration and particle size distribution were analysed using drying–weighing and a particle-size analyser (Mastersizer 2000), respectively. The experimental results indicated that an increase in feed-flow rate from 2 to 4 m3/hr produced decreased separation efficiency. However, when the feed-flow rates increased from 4 to 6 m3/hr, the separation efficiency increased. Furthermore, the higher the feed-flow rate, the smaller the cut size. A novel separation efficiency equation in terms of the concentration ratio and flow ratio is also proposed.

Bioethanol Production from Oil Palm Frond by Simultaneous Saccharification and Fermentation

Ethanol production from oil palm frond (OPF) by simultaneous saccharification and Saccharomyces cerevisiae TISTR5048 fermentation was investigated. Solid fraction of OPF (20% TS) was pretreated by 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking at room temperature for 24hours. Pretreated OPF by presoaking in 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 contained 37%, 42% and 49% of cellulose, respectively. Pretreated OPF was simultaneous saccharification by cellulase enzyme (Cellic CTec2, Novozymes) and sequentially fermentation. Sugar concentration in OPF cellulose hydrolysis of 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking was 45.72, 55.73 and 56.94g/l, respectively. Ethanol concentration of 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking was 14.5, 15.0 and 17.2g/L, respectively. 2% NaOH in H2O2 presoaking was the best pretreatment with 82.11% of total solids recovery and containing 49.9% of cellulose with enzyme digestion ability of 37.6%.

Physical, Compositional, and Wet‐Milling Characteristics of Mexican Blue Maize (Zea mays L.) Landrace

Mexico has the largest diversity of genetic resources for maize in the world, with about 59 different landraces. However, little is known about their wet‐milling characteristics. The aim of this study was to determine whether 15 Mexican blue maize (Zea mays L.) genotypes of Elotero de Sinaloa landrace collected in the northwestern region of Mexico have suitable wet‐milling properties. Great variability of physical, compositional, and wet‐milling characteristics among these blue maize genotypes was observed. The FAUAS‐457 and FAUAS‐488 maize genotypes had similar starch yield and starch recovery as reported for the wet‐milling industry, which indicated that they may be useful as a source of extractable starch. Residual protein levels in the starch fractions were in the range of 0.39–0.68%, and total solids recovery exhibited a mean value of 98.8%, indicating acceptable efficacy of the wet‐milling process. This process afforded starches from blue maize genotypes with low protein contents. Wet‐milling fractions correlated with the physical and chemical properties of the kernels. Our results indicate that Mexican blue maize genotypes contain characteristics that make them appropriate and utilizable at the industrial level, and they can also be valuable for improving wet‐milling characteristics of maize through breeding programs.

Utilization of spent brewer’s yeast Saccharomyces cerevisiae for the production of yeast enzymatic hydrolysate

Spent brewer’s yeast (Saccharomyces cerevisiae) is a rich source of protein, vitamins and widely used as a raw material for production of food supplements. The autolysis and enzymatic treatment of spent brewer’s yeast using Pancreatin (2.5%) and Flavourzyme (2.5%) were performed at 45 °C and 50 °C, respectively. The autolysis and hydrolysis processes were evaluated by determining a soluble solids, soluble protein concentration and α-amino nitrogen content in a reaction mixture. The yield of pancreatic digest and α-amino nitrogen content was high in comparison with autolysis and Flavourzyme treatment. The total solids recovery in dry Yeast hydrolysate was about 50%, a protein and α-amino nitrogen content was 55.9 and 4.8%, respectively. These results show the possibility of utilizing the spent brewer’s yeast as hydrolysate using hydrolytic enzymes and use it as a food supplement after biological experiments.DOI: http://dx.doi.org/10.5564/mjc.v12i0.179 Mongolian Journal of Chemistry Vol.12 2011: 88-91

Yield Optimization of Spray‐Dried Natural Rubber and Properties of Its Silica‐Filled Composite

ABSTRACTNatural rubber powder (NRP) was prepared by spray drying and optimized via statistical experimental design. The yield of NRP obtained significantly depended on the percentage of dry rubber content (DRC) and the amount of sodium dodecyl sulfate (SDS). The optimum condition for spray drying was found to be with an inlet air temperature of 130°C, a feed rate of 4 mL min−1, nozzle flow rate of 600 L h−1, 15% DRC, and 12 parts per hundred of rubber (phr) of SDS. Under these conditions, a NRP yield of 44.9%, and a total solid recovery of 98.7% with a moisture content of 0.4% was obtained. The resultant NRP particles were almost spherical with a diameter of less than 10 μm. The mechanical properties of NRP, in terms of the modulus, tensile strength, and hardness were greater than that for conventional block rubber (STR). Moreover, the addition of silica filler (25 phr) yielded better mechanical properties when prepared by spray drying (Si/NRP) than by the conventional silica‐filled block rubber (Si/STR). Scanning electron microscopy revealed that the silica was more remarkably evenly dispersed in the rubbery matrix of the Si/NRP composite compared to the agglomerated clumps found in the Si/STR composite prepared by the conventional mechanical mixing method.

Pretreatment of oil palm empty fruit bunch (OPEFB) by N-methylmorpholine-N-oxide (NMMO) for biogas production: Structural changes and digestion improvement

Pretreatment of OPEFB (oil palm empty fruit bunch) by NMMO (N-methylmorpholine-N-oxide) on its subsequent digestions was investigated. The pretreatments were carried out at 90 and 120°C for 1, 3, and 5h in three different modes of dissolution (by 85% NMMO solution), ballooning (79% NMMO solution), and swelling (73% NMMO solution). The total solid recovery after the pretreatment was 89–94%. The pretreatment process did not have a major impact on the composition of OPEFB, other than a reduction of ash from 5.4% up to 1.3%. The best improvement in biogas production was achieved by a dissolution mode pretreatment of OPEFB, using conditions of 85% NMMO, 3h, and 120°C. It resulted in 0.408Nm3/kg VS methane yield and 0.032Nm3CH4/kg VS/day initial methane production rate, which correspond in improving by 48% and 167% compared to the untreated OPEFB, respectively.

Effect of different coagulants at varying strengths on the quality of paneer made from reconstituted milk

A study was conducted to determine the effect of different types of acids viz., citric acid, tartaric acid and malic acid each at 2, 3 and 5% concentrations on the quality of paneer made using reconstituted milk. The moisture, total solid recovery and yield and sensory scores for flavour, body and texture and overall acceptability of paneer decreased with the increasing strength of acid. However, these parameters for paneer made using coagulants at 2 and 3% levels were statistically comparable (P > 0.05). Fat and protein per cent increased with the increase in the concentration of the acid. No difference was observed in the levels of ash and fat on dry matter basis and pH and appearance scores at all the three concentrations of the coagulants. The type of coagulant also elicited variations in most of the constituents of paneer. The paneer samples made with citric acid and tartaric acid had significantly higher (P ≤ 0.05) values for fat, protein, ash, total solids recovery, fat on dry matter basis, body and texture and overall acceptability scores than paneer made with malic acid at all concentrations. No significant difference was seen in appearance and flavour scores among all the samples. In order to produce paneer with the most desirable characteristics from reconstituted milk, it is suggested citric acid and tartaric acid at 2% concentration can be utilized as coagulants.

Preliminary evaluation of organosolv pre-treatment of sugar cane bagasse for glucose production: Application of 2 3 experimental design

Sugar cane bagasse was submitted to ethanol organosolv pre-treatment using a 50 L pilot scale reactor. The influence of catalyst type (H 2SO 4 or NaOH), catalyst concentration (1.25–1.50% w/w on dry fiber) and process time (60–90 min) on total solid recovery and solid composition (glucan, xylan and lignin contents) was evaluated by performing a 2 3 full factorial experimental design. Pretreated sugar cane bagasse was further submitted to enzymatic hydrolysis using a commercial enzyme complex formed by cellulases and β-glucosidases. Glucose concentration in the hydrolysates and glucose yield referred to initial raw material (g glucose/100 g sugar cane bagasse) were used to select the best operational conditions. Concerning the enzymatic hydrolysis, the resulting glucose concentration was found to be dependent on xylan contents of the pretreated material. The modelling equations for glucose concentration and glucose yield as a function of the pre-treatment variables and the statistical analysis are also discussed in this work.