Total SCFA Concentrations Research Articles

The effect of supplemental arginine on the gut microbial homeostasis of broilers during sub-clinical necrotic enteritis challenge.

Necrotic enteritis (NE) is an enteric disease of poultry that alters the structure of the gut microbial community causing dysbiosis. This 28day experiment investigated the effects of 125% and 135% arginine diets on the gut microbial diversity and composition of broilers during a subclinical NE challenge. One hundred and twenty one-day-old chicks were randomly allocated to 4 treatments with six replicates each- Uninfected + Basal, NE + Basal, NE + Arg 125%, and NE + Arg 135% diet groups. NE was induced by inoculating 1 × 104E. maxima sporulated oocysts on day 14 and 1 × 108CFUC. perfringens on days 19, 20, and 21 of age. The NE challenge significantly decreased the number of observed amplicon sequence variants (p = 0.03), the abundance of the phylum Firmicutes (p < 0.01), and the species Mediterraneibacter cottocaccae (p = 0.01) in the ceca of birds on day 21. The NE challenge significantly increased the Bray-Curtis index (p < 0.01), and the abundance of the phylum Bacteroidota (p < 0.01), family Odoribacteraceae (p < 0.01), genus Odoribacter (p < 0.01), and species O. splanchnicus (p = 0.01) on day 21. During NE, the 125% arginine diet restored the abundance of the phylum Bacteroidota (p = 0.03), family Odoribacteraceae (p = 0.03) and Oscillospiraceae (p = 0.03), genus Odoribacter (p = 0.03), and species O. splanchnicus (p = 0.03) and M. cottocaccae (p < 0.01) on day 21. The 135% arginine diet effectively restored the loss in alpha diversity (p = 0.01) caused by NE, the abundance of the phylum Firmicutes (p = 0.01) and Bacteroidota (p < 0.01), family Oscillospiraceae (p = 0.03) and Odoribacteraceae (p < 0.01), genus Odoribacter (p < 0.01), and species O. splanchnicus (p < 0.01) and M. cottocaccae (p < 0.01) on day 21. On day 28, the treatments had a significant effect on the cecal propionate (p = 0.01), butyrate (p = 0.04), and total SCFA (p = 0.04) concentrations. In conclusion, the 125% and 135% arginine diets restored gut microbial composition during a subclinical NE challenge, but not the cecal SCFA profile. Hence, arginine in combination with other feed additives could be used in restoring gut microbial homeostasis during NE in poultry.

Interaction of Wheat Bran Particle Size and Stimbiotic Supplementation on Growth Performance and Gut Health Parameters in Broilers.

A 42-day study was conducted with 720-day-old Cobb male broiler chicks allocated to treatments in a 3 × 2 factorial, with the factors as wheat bran (WB) inclusion (no WB, 50 g/kg coarse WB, or 50 g/kg fine WB) and stimbiotic (STB) supplementation in corn-based diets. The inclusion of WB (p < 0.05) or STB supplementation (p < 0.05) increased the FCR and feed intake in the day 0-10 phase. During the day 0-28 phase, coarse-WB inclusion increased (p < 0.05) the FCR, compared to fine WB or diets without WB. In the day 0-42 phase, WB marginally decreased weight gain in diets without STB supplementation, but the STB-supplemented diet, weight gain was greater (p < 0.05) the diet with fine WB compared with diets with coarse WB. Fine-WB inclusion increased the ileal nitrogen and energy digestibility determined at day 18 compared to coarse WB or diets without WB. Supplementation with STB (p < 0.05) or fine WB (p < 0.05) inclusion increased the villi height compared to diets without STB supplementation or coarse WB, or the diet without WB. Coarse or fine WB decreased (p < 0.05) cecal branched-chain fatty acids compared to diets without WB. In conclusion, stimbiotic supplementation to fine WB improved the performance and nutrient digestibility of broilers compared to coarse WB with no effects on the caeca total SCFA concentration.

Performance and ruminal fermentation of second-trimester pregnant beef cows fed short-season high-moisture corn stover or barley greenfeed during winter in western Canada

This study evaluated performance and ruminal fermentation for cows fed short-season high-moisture shelled corn stover with corn dried distillers’ grains with solubles (COR) or barley greenfeed (BAR) that was either swathed (S-COR; S-BAR; Experiment 1) or baled (B-COR; B-BAR; Experiment 2) as winter-feeding systems. In Experiment 1, cows were randomly assigned to S-COR or S-BAR and fed for 52 days in the fields where crops were grown. Body weight (BW), subcutaneous rib and rump fat, and body condition score (BCS) were measured, with no differences detected between treatments ( P &gt; 0.05). However, S-COR had a lower estimated dry matter intake (DMI) than S-BAR ( P ≤ 0.03). In Experiment 2, cows were assigned to B-COR or B-BAR for 42 days and fed in field paddocks. Cows fed B-COR had less ( P ≤ 0.01) DMI, final BW, rib fat, rump fat, and BCS than B-BAR, with no differences ( P &gt; 0.05) for ruminal pH. Total SCFA concentration was greater ( P ≤ 0.05) on day 21 for B-BAR than B-COR, but not on day 42. Under western Canadian conditions, COR may reduce DMI and the performance of pregnant cows, suggesting that additional preservation and supplementation strategies should be investigated.

Diet medication and beta-glucanase affect ileal digesta soluble beta-glucan molecular weight, carbohydrate fermentation, and performance of coccidiosis vaccinated broiler chickens given wheat-based diets

Exogenous enzymes as alternatives to feed antibiotics in poultry has become an emerging research area with the emergence of antibiotic resistance. The objective was to evaluate the effects of diet medication (antibiotics) and β-glucanase (BGase) on digesta soluble β-glucan depolymerization, carbohydrate fermentation, and performance of coccidiosis-vaccinated broiler chickens fed wheat-based diets. A total of 1,782 broilers were raised on litter floor pens, and each treatment was assigned to 1 pen in each of the 9 rooms. The 3 dietary treatments were based on wheat as the sole grain (control, control + medication and control + 0.1% BGase), and the birds were fed the respective treatments ad libitum from 0 to 33 d. Treatments were arranged in a randomized complete block design and analyzed as a one-way ANOVA. Beta-glucanase reduced the peak molecular weight, weight average molecular weight (Mw) and maximum molecular weight for the smallest 10% β-glucan molecules (MW-10%) in ileal digesta at d 11 and 33, whereas diet medication reduced Mw and MW-10% at d 33 compared to the control (P < 0.01). Beta-glucanase and medication reduced the ileal viscosity at d 11 compared to the control (P = 0.010). Ileal propionic acid concentration at d 11 and caecal total SCFA, acetic, and butyric acid concentrations at d 33 were lower in the BGase-supplemented diet than in the control (P < 0.05). The BGase-added diet had higher duodenal pH compared to the control at d 33 (P = 0.026). The effect of medication on carbohydrate fermentation was minimal. Diet medication increased weight gain after d 11, whereas BGase increased the gain for the total trial period compared to the control (P < 0.001). Feed intake was not affected by the dietary treatment. Medication and BGase improved feed efficiency after d 11 compared to the control (P < 0.001). The response to diet medication was larger than BGase, considering weight gain and feed efficiency after d 11 (P < 0.001). In conclusion, diet medication and BGase depolymerized high molecular weight ileal soluble β-glucan and increased overall bird performance. Dietary BGase may benefit bird health in broilers fed wheat-based diets without medication.

Effect of Post-Ruminal Urea Supply on Growth Performance of Grazing Nellore Young Bulls at Dry Season.

The objective of this study was to evaluate the effect of the use of post-ruminal urea on performance, nitrogen metabolism and the ruminal environment of Nellore cattle reared on pasture during the dry season. In experiment 1 (Exp. 1), nine ruminal-cannulated Nellore steers, 30 ± 2 months old (651 ± 45 kg body weight (BW)), were allotted to a 3 × 3 Latin triple square. In experiment 2 (Exp. 2), 84 Nellore bulls, 18 ± 3 months old (315 ± 84 kg BW), were distributed in complete randomized blocks, by initial BW. Protein supplements were supplied daily, in the amount of 2 g/kg BW, and consisted of either CONT: protein + conventional urea (50% CP), PRU: protein + post ruminal urea (50% CP) and U + PRU: protein + urea conventional + post-ruminal urea (70% CP). The paddocks were composed of Urochloa brizantha cv. Marandu grass. In Exp. 1, there was no treatment effect for DM, OM, NDF, forage intake, and CP, but there was a higher intake for PRU (p &lt; 0.005) and a higher digestibility for U+ PRU (p = 0.001). There was no effect on ruminal pH or NH3-N concentration (p ≥ 0.232), but there was an interaction between treatment and time for them (p &lt; 0.039). Furthermore, there was a treatment effect on the total SCFA concentration, with CONT being higher than the others. A difference in the acetate:propionate ratio was found (p &lt; 0.027), with a greater relationship for PRU and U + PRU. A treatment effect (p = 0.049) was found for the propionate proportion, with a higher proportion in the CONT. Nitrogen intake was consequently lower for the CONT and higher urinary excretion for the U + PRU (p = 0.002). Animals supplemented with CONT showed a tendency to have more Bacteria and fewer Archaea (p = 0.086). In Exp. 2, there was a treatment effect for the disappearance rate of the supplement (p &lt; 0.001). Intake was faster for PRU and CONT, but performance was not affected by PRU (p = 0.311). The use of post-ruminal urea alters the microbial population, but does not affect performance. Therefore, supplementation with post-ruminal urea presented similar results compared to conventional urea. Ruminal and blood parameters and animal performance were not influenced by treatments.

Effect of forage types differing in undigested neutral detergent fiber concentration and forage inclusion rate on reticulo-ruminal motility and fermentation, total tract barrier function, and blood metabolites of finishing beef heifers.

This study evaluated the effects of forages (BarS vs. STR) that differ in the uNDF concentration and FI rate on ruminal fermentation, total tract barrier function, reticulo-ruminal motility, and blood metabolites of beef heifers. Six ruminally cannulated Hereford × Simmental heifers (699 ± 69.1 kg BW) were used in a 6 × 6 Latin square (26 d periods) with a 2 × 3 factorial treatment arrangement. However, 1 heifer was removed from the study after period 2 due to health problems unrelated to treatment, resulting in an incomplete 6 × 6 Latin square design. Barley grain-based diets were formulated using BarS or wheat STR to alter uNDF (7.1% vs. 8.5% DM) with FI rates of 5%, 10%, or 15% of DM. There were limited interactions between the forage type and FI. DM intake was not affected (P ≥ 0.10) by forage type or FI. Use of STR vs. BarS increased uNDF intake (P < 0.001). Increasing FI increased (P < 0.001) uNDF intake for those fed 15% forage. Ruminal pH was not affected (P ≥ 0.10) by forage type; however, cattle fed 5% FI had lesser (P = 0.017) mean ruminal pH and maximum pH (P = 0.018) than those fed 10% and 15% of forage. The total SCFA concentration was not affected by forage type (P = 0.84) but cattle fed the 5% FI rate had lesser (P < 0.001) molar proportion of acetate when compared with cattle fed 10% and 15% forage. Increasing the FI rate decreased the molar proportion of propionate (P < 0.001). Feeding STR relative to BarS decreased (P = 0.041) the reticulo-ruminal contraction duration. In contrast, cattle fed the 10% and 15% FI rates had a greater (P = 0.028) contraction frequency with lower (P = 0.048) contraction area than those fed 5% forage. Plasma glucose, serum insulin, and serum amyloid A were not affected by forage type or FI rate (P ≥ 0.10). Cattle fed 15% forage had lesser (P = 0.040) concentration of serum haptoglobin when compared with cattle fed 5% or 10% forage. In conclusion, forage type used to affect the dietary uNDF concentration, and FI rate act independently suggesting that the provision of STR to increase uNDF reduces reticulo-ruminal contraction duration and total tract permeability but may not affect ruminal pH. Increasing the FI increased dietary uNDF, stabilized ruminal pH, stimulated more frequent reticulo-ruminal contractions, and may decrease the permeability of the gastrointestinal tract and systemic inflammation.

Associations of Dietary Intake with the Intestinal Microbiota and Short-Chain Fatty Acids Among Young Adults with Type 1 Diabetes and Overweight or Obesity

BackgroundDiet, a key component of type 1 diabetes (T1D) management, modulates the intestinal microbiota and its metabolically active byproducts—including SCFA—through fermentation of dietary carbohydrates such as fiber. However, the diet–microbiome relationship remains largely unexplored in longstanding T1D. ObjectivesWe evaluated whether increased carbohydrate intake, including fiber, is associated with increased SCFA-producing gut microbes, SCFA, and intestinal microbial diversity among young adults with longstanding T1D and overweight or obesity. MethodsYoung adult men and women with T1D for ≥1 y, aged 19–30 y, and BMI of 27.0–39.9 kg/m2 at baseline provided stool samples at baseline and 3, 6, and 9 mo of a randomized dietary weight loss trial. Diet was assessed by 1–2 24-h recalls. The abundance of SCFA-producing microbes was measured using 16S rRNA gene sequencing. GC-MS measured fecal SCFA (acetate, butyrate, propionate, and total) concentrations. Adjusted and Bonferroni-corrected generalized estimating equations modeled associations of dietary fiber (total, soluble, and pectins) and carbohydrate (available carbohydrate, and fructose) with microbiome-related outcomes. Primary analyses were restricted to data collected before COVID-19 interruptions. ResultsFiber (total and soluble) and carbohydrates (available and fructose) were positively associated with total SCFA and acetate concentrations (n = 40 participants, 52 visits). Each 10 g/d of total and soluble fiber intake was associated with an additional 8.8 μmol/g (95% CI: 4.5, 12.8 μmol/g; P = 0.006) and 24.0 μmol/g (95% CI: 12.9, 35.1 μmol/g; P = 0.003) of fecal acetate, respectively. Available carbohydrate intake was positively associated with SCFA producers Roseburia and Ruminococcus gnavus. All diet variables except pectin were inversely associated with normalized abundance of Bacteroides and Alistipes. Fructose was inversely associated with Akkermansia abundance. ConclusionsIn young adults with longstanding T1D, fiber and carbohydrate intake were associated positively with fecal SCFA but had variable associations with SCFA-producing gut microbes. Controlled feeding studies should determine whether gut microbes and SCFA can be directly manipulated in T1D.

Mangrove fruit (Bruguiera gymnorhiza) increases circulating GLP-1 and PYY, modulates lipid profiles, and reduces systemic inflammation by improving SCFA levels in obese wistar rats

Bruguiera gymnorhiza (BG) has potential as a functional food because of its dietary fibre content and bioactive components such as flavonoids and phenolic compounds. However, it is not studied in the context of diet-related disease prevention. In the present study, we aimed to investigate the effects of Bruguiera gymnorhiza fruit flour (BGF) on satiety hormone, lipid profile, systemic inflammation, body weight, and caecum SCFA levels in diet-induced obese rats. A total of 28 obese male Wistar rats were divided into four groups. Group 1 (K1) was given a standard chow, group 2 (K2) standard chow + orlistat, group 3 (P1) standard chow + BGF 2 g/200 g BW/day, and group 4 (P2) standard chow + BGF 4 g/200 g BW/day for 28 days. The levels of GLP-1, PYY, total cholesterol (TC), triglyceride (TG), HDL, IL-6, TNF-α, and body weight were measured before and after the intervention; meanwhile, the caecum SCFA levels were assessed only after the intervention. In this study, BGF intervention increased the dose-dependent plasma GLP-1 and PYY levels (P < 0.000). In addition, BGF intervention also decreased lipid profiles (TC & TG) (P < 0.000, respectively) and systemic inflammation in a dose-dependent manner. Finally, acetate, propionate, and total SCFA concentrations were higher in the BGF intervention group (P2) compared to the other groups (p < 0.05). The SCFA levels were associated with satiety hormones, lipids, and systemic inflammation (P < 0.05). The BGF intervention improved satiety hormone, lipid profile, systemic inflammation, and SCFA levels.

Effects of a low FODMAP diet on the colonic microbiome in irritable bowel syndrome: a systematic review with meta-analysis

ABSTRACTBackgroundA low fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAP) diet is increasingly used to manage symptoms in irritable bowel syndrome (IBS). Although this approach may alter the colonic microbiome, the nature of these changes has not been comprehensively synthesized.ObjectivesThe aim of this study was to conduct a systematic review with meta-analysis of randomized controlled trials examining the impact of a low FODMAP diet on the composition and function of the microbiome in patients with IBS.MethodsA systematic search was conducted for randomized controlled trials evaluating the effects of a low FODMAP diet on the colonic microbiome in patients with IBS in MEDLINE, EMBASE, CENTRAL, and Web of Science from inception to April 2022. Outcomes included diversity of the microbiome, specific bacterial abundances, fecal SCFA concentration, and fecal pH. For fecal SCFA concentrations and pH, meta-analyses were performed via a random-effects model.ResultsNine trials involving 403 patients were included. There were no clear effects of the low FODMAP diet on diversity of the microbiome. A low FODMAP diet consistently led to lower abundance of Bifidobacteria, but there were no clear effects on diversity of the microbiome or abundances of other specific taxa. There were no differences in total fecal SCFA concentration between the low FODMAP diet and control diets (standardized mean difference: −0.25; 95% CI: −0.63, 0.13; P = 0.20), nor were there differences for fecal concentrations of specific SCFAs or fecal pH.ConclusionsIn patients with IBS, the effects of a low FODMAP diet on the colonic microbiome appear to be specific to Bifidobacteria with no consistent impacts on other microbiome metrics, including diversity, fecal SCFA concentrations, and fecal pH. Further, adequately powered trials are needed to confirm these findings.This review was registered at https://www.crd.york.ac.uk/prospero/ as CRD42020192243.

High throughput in vitro characterization of pectins for pig(let) nutrition

BackgroundFiber-rich feed components possess prebiotic potential to enhance pig health and are considered a potential solution to the high prevalence of post-weaning diarrhea in pig production under the phased suspension of antibiotics and zinc oxide use.MethodsWe screened the gut microbiota modulatory properties of pectin substrates prepared from sugar beet within the freshly weaned piglet gut microbiome using an in vitro colon model, the CoMiniGut. We focused on testing a variety (13) of sugar beet-derived pectin substrates with defined structures, as well as known prebiotics such as inulin, fructooligosaccharide (FOS) and galactooligosaccharide (GOS), to gain insights on the structure–function related properties of specific substrates on the weaner gut microbial composition as well as shortchain fatty acid production (SCFA).ResultsSugar beet-derived pectin and rhamnogalacturonan-I selectively increased the relative abundance of Bacteroidetes, specifically Prevotella copri, Bacteroides ovatus, Bacteroides acidificiens, and an unclassified Bacteroides member. The degree of esterification impacted the relative abundance of these species and the SCFA production during the in vitro fermentations. Modified arabinans derived from sugar beet promoted the growth of Blautia, P. copri, Lachnospiraceae members and Limosilactobacillus mucosae and amongst all oligosaccharides tested yielded the highest amount of total SCFA produced after 24 h of fermentation. Sugar beet-derived substrates yielded higher total SCFA concentrations (especially acetic and propionic acid) relative to the known prebiotics inulin, FOS and GOS.ConclusionOur results indicate that the molecular structures of pectin, that can be prepared form just one plant source (sugar beet) can selectively stimulate different GM members, highlighting the potential of utilizing pectin substrates as targeted GM modulatory ingredients.

Wheat bran with reduced particle size increases serum SCFAs in obese subjects without improving health parameters compared with a maltodextrin placebo

Wheat bran (WB) has been associated with improved gastrointestinal health and a reduced risk of metabolic disorders. Reducing the particle size of WB might increase its fermentability and facilitate cross-feeding between the gut bacteria and in this way produce health effects. We investigated the impact of WB with reduced particle size (WB RPS) on colonic fermentation and host health in normal-weight (NW) and obese (OB) participants compared with placebo (PL). During 1 mo, 36 NW and 14 OB participants daily consumed 20 g WB RPS or PL (maltodextrin). Before and after the intervention, fasting serum and fecal SCFAs, fecal metabolite profiles, and microbiota composition were measured as fermentation parameters. Fecal output, fecal dry weight (%), fat excretion, transit, stool consistency, intestinal permeability, and serum total cholesterol, triglyceride, and C-reactive protein concentrations were measured as health parameters. The impact of WB RPS on the fermentation of other carbohydrates was assessed by quantifying postprandial cumulative serum 13C-SCFA after a challenge with 13C-inulin. WB RPS increased fasting serum acetate (P<0.05) and total SCFA (P<0.05) concentrations in OB participants. Fasting serum propionate concentrations were lower in OB than in NW participants at baseline (NW: 1.57±0.75 µmol/L; OB: 0.89±0.52 µmol/L; P<0.01), but not after WB RPS (NW: 1.75±0.77 µmol/L; OB: 1.35±0.63 µmol/L; P=not significant). WB RPS did not enhance colonic fermentation of 13C-inulin and did not affect microbiota composition. Health parameters were not affected by the WB RPS intervention, either in NW or in OB participants. WB RPS increased fasting serum SCFA concentrations in OB participants. These changes were not associated with beneficial effects on host health.

Low doses of monensin for lambs fed diets containing high level of ground flint corn

Two experiments were proposed to evaluate the addition of monensin for lambs fed diets containing a high level of mature ground flint corn. The experimental diets were as follows: no inclusion of monensin (M0) and inclusion of 8 (M8), 16 (M16) and 24 mg kg–1 of monensin (M24). In experiment 1, eight cannulated wethers were divided into a double 4 × 4 Latin square experimental design to evaluate nutrient digestibility, plasma parameters and rumen fermentation. The experiment lasted 112 days, divided into four periods of 28 days each. In experiment 2, ninety-two lambs were used in a randomized block design to evaluate the performance over 56 days. In experiment 1, doses of monensin had no effect on nutrient intake (p ≥ 0.07) and digestibility (p ≥ 0.09). There was a quadratic effect for acetate molar proportion (p = 0.01), acetate to propionate ratio (p = 0.04) and rumen pH (p < 0.01). However, there was no effect on the molar proportion of propionate and butyrate. The monensin decreased linearly the total SCFA concentration (p < 0.01). The inclusion of monensin increased glucose (p < 0.01) and decreased lactate concentration in plasma (p = 0.05). In experiment 2, monensin decreased dry matter intake (p = 0.04). However, there was a quadratic effect for average daily gain (p = 0.03) and feed efficiency (p < 0.01), with the greatest values observed for the M8 diet. Thus, the inclusion of 8 mg kg–1 of dry matter diet (DM) improves ruminal fermentation and plasma parameters, resulting in greater growth performance in lambs.

Effects of guanidinoacetic acid and coated folic acid supplementation on growth performance, nutrient digestion and hepatic gene expression in Angus bulls.

To evaluate the impacts of guanidinoacetic acid (GAA) and coated folic acid (CFA) on growth performance, nutrient digestion and hepatic gene expression, fifty-two Angus bulls were assigned to four groups in a 2 × 2 factor experimental design. The CFA of 0 or 6 mg/kg dietary DM folic acid was supplemented in diets with GAA of 0 (GAA-) or 0·6 g/kg DM (GAA+), respectively. Average daily gain (ADG), feed efficiency and hepatic creatine concentration increased with GAA or CFA addition, and the increased magnitude of these parameters was greater for addition of CFA in GAA- diets than in GAA+ diets. Blood creatine concentration increased with GAA or CFA addition, and greater increase was observed when CFA was supplemented in GAA+ diets than in GAA- diets. DM intake was unchanged, but rumen total SCFA concentration and digestibilities of DM, crude protein, neutral-detergent fibre and acid-detergent fibre increased with the addition of GAA or CFA. Acetate:propionate ratio was unaffected by GAA, but increased for CFA addition. Increase in blood concentrations of albumin, total protein and insulin-like growth factor-1 (IGF-1) was observed for GAA or CFA addition. Blood folate concentration was decreased by GAA, but increased with CFA addition. Hepatic expressions of IGF-1, phosphoinositide 3-kinase, protein kinase B, mammalian target of rapamycin and ribosomal protein S6 kinase increased with GAA or CFA addition. Results indicated that the combined supplementation of GAA and CFA could not cause ADG increase more when compared with GAA or CFA addition alone.

FORTIFIED DADIH (FERMENTED BUFFALO MILK) WITH VITAMIN D3 IMPROVES INTERLEUKIN-6 AND CAECUM SHORT CHAIN FATTY ACIDS ON DIET-INDUCED OBESE RAT

Objective: This study aims to determine the effect of fortified dadih with vitamin D3 on IL-6 expression level and the concentration of caecum SCFA in obese rats.&#x0D; Methods: A total of 30 male Sprague Dawley rats were divided into five equal groups: healthy-control-(K-), obese-control-(K+), obese-intervention-(X1, X2, and X3). K(+), X1, X2, and X3 were in obesity conditions, which was induced by a high-fat sucrose diet (HFSD) and K(-) as a healthy-control-group. Furthermore, vitamin D3-fortified dadih at doses of 4 g/200 g-body-weight/d, dadih only at doses of 4 g/200 g-body-weight/d, and vitamin D3 only at 36 IU/200 g-body-weight/d was administered to X1, X2, and X3 groups, respectively.&#x0D; Results: Treatment using fortified dadih with vitamin D3 showed significantly reduce weight gain (p&lt;0.05) compare to K(+) and X2. In addition, X1 showed a decreased level of Interleukin-6 expression (p&lt;0.05) than K(+), X2, and X3 groups but higher than K(-). Also, it showed the highest total SCFA, acetate, and propionate concentration (p&lt;0.05). However, a moderately negative correlation was discovered between the pair of total SCFA and Interleukin-6 expression, acetate and Interleukin-6 expression, SCFA and body weight, propionate and body weight, butyrate and body weight. On the contrary, a strong positive correlation was observed between the pair of Interleukin-6 expression levels and body weight.&#x0D; Conclusion: This study shows that fortified dadih with vitamin D3 from fermented foods improve the expression level of Interleukin-6 and increase the production of SCFA. Also, they improve intestinal homeostasis because of the increased SCFA production.

Residual effect of narasin on ruminal fermentation characteristics in lambs

This study aimed to evaluate the effect of narasin withdrawal on wethers ruminal fermentation characteristics. Four wethers cannulated at the rumen were subjected to a 4 × 4 Latin Square experimental design. The trial lasted 144 days, divided into 4 periods of 36 days each. The diet was composed by 950 g/kg of coastcross hay and 50 g/kg of ground corn, used as a carrier for the narasin. The experimental diets were defined by frequency of narasin offer: 1) no feed additives (N0); 2) 13 mg of narasin/kg of DM every 24 h (N13); 3) 26 mg of narasin/kg of DM every 48 h (N26), and 4) 39 mg of narasin/kg of DM every 72 h (N39). The narasin was offered for 25 days, and at the last 11 days of each period, all the animals received the same proportion of forage and concentrate, but without narasin inclusion (withdrawal period). Last day of narasin offering was denominated day 0 and the first day of withdrawal day 1. The fed offer and orts were daily recorded to determine de DMI and nutrient intake. Ruminal fluid was collected 6 h after concentrate offering from d0 to d11 to determine ruminal pH, SCFA and ammonia nitrogen. There was no difference for dry matter intake among treatments after narasin withdrawal. There was no treatment and day interaction for total SCFA concentration, molar proportion of acetate, butyrate, isobutyrate, valerate, isovalerate and rumen pH. Treatment and day interaction after narasin withdrawal interactions was observed for molar proportion of propionate (P < 0.01), Ac:Prop ratio (P = 0.03) and ammonia nitrogen concentration (P = 0.02). The increase in molar proportions of propionate for all narasin-containing treatments compared with the control was no significant by day 5 after narasin withdrawal (P ≥ 0.13). After narasin withdrawal, all doses of narasin inclusion decreased Ac:Prop ratio until day 3 after withdrawal (P ≤ 0.05) when compared with control diet. Effect of narasin on ruminal ammonia nitrogen concentration remained until day 3 after withdrawal (P ≤ 0.04), from day 4 there was no treatments effect (P ≥ 0.11) on ammonia nitrogen concentration. The data obtained from the present study demonstrate that narasin has a prolonged effect after withdrawal from the diet, being observed an effect on ruminal fermentation characteristics within 4 days after narasin withdrawal. These data suggest that the residual effect of narasin can be used as strategic feeding in situations where daily supplementation is not possible. Additionally, concerning scientific research using reversal designs, there is a need for a minimum of 5 days of washout between the experimental periods.

Dietary Hemp Seeds More Effectively Attenuate Disorders in Genetically Obese Rats than Their Lipid Fraction

Hemp seeds are rich in PUFAs and other bioactives that can attenuate the development of obesity-related disorders; however, the extent to which their lipid fraction is responsible for this effect is unknown. We hypothesized that hemp seed or hemp oil supplementation can attenuate genetically determined disorders and that the former are more effective in doing so. Lean and obese male Zucker rats, aged 8 wk, weighing 174±4.2g and 223±3.8g, respectively, were allocated to 4 groups. The lean (LC) and obese controls (OC) were fed a standard diet, whereas the other 2 obese groups were fed a modified diet in which hemp oil (4% diet; O+HO) or hemp seeds (12% diet; O+HS) were included. All diets had the same proportions of protein (18%), fat (8%), and fiber (5%) and a similar carbohydrate proportion (∼52%). Diets fed to O+HO and O+HS had similar fatty acid profiles. After 4 wk, markers of gut and liver function, antioxidant status, and lipid metabolism were measured. The total SCFA concentration in the cecal digesta was lower in OC (64.8±4.21µmol/g) compared with LC (78.1±2.83µmol/g) (P≤0.05), whereas it was greater in O+HS (89±4.41µmol/g) compared with LC, OC, and O+HO (69.7±2.68µmol/g) (P≤0.05). Plasma total cholesterol was greater in OC (6.20±0.198mmol/L) and O+HO (5.60±0.084mmol/L) compared with LC (2.71±0.094mmol/L) (P≤0.05); in O+HS, the concentration did not differ from the other groups (5.16±0.278mmol/L). The liver cholesterol concentration was greater in OC (1.79±0.379mg/g) compared with the other groups (1.28-1.43mg/g) (P≤0.05). Hepatic expression of peroxisome proliferator-activated receptor γ was lower in OC (11.9±0.93 units) compared with LC (17.3±1.3 units) (P≤0.05), whereas it was greater in O+HS (19.2±1.04 units) compared with OC and O+HO (14.0±1.33 units) (P≤0.05). Dietary hemp seeds more effectively attenuate metabolic disorders in genetically obese rats than the oil extracted from them, which suggests that the lipid fraction is only partly responsible for these effects.

Effect of Hydrothermal Treatment of Depigmented Turmeric (Curcuma longa L.) on Cecal Fermentation in Rats

AbstractRaw depigmented turmeric powder (TP) and hydrothermally treated TP (HT‐TP) contain non‐digestible saccharides with fiber and RS contents of 53.3% and 39.9%, respectively. Starch granules in TP and HT‐TP are observed under a petrographic microscope. The native and partly digested starch granules hydrolyzed by digestive enzymes, are also observed under SEM. In an animal study, cecal total SCFA concentration in rats fed 10% TP diet followed by 10% HT‐TP diet is (p &lt; 0.05) higher than the control diet, while acetate and n‐butyrate concentrations in 10% HT‐TP diet are (p &lt; 0.05) lower than in the 10% TP diet, which is still higher than the control diet. These data indicate that starch in HT‐TP is resistant to gelatinization and/or enzymatic digestion; thus, HT‐TP may be a useful food material conducive for increasing intestinal fermentation.

84 Effect of incorporating forage pea (c.v. Pisium sativum L.) into cereal hay on ruminal fermentation and apparent digestibility when fed to beef heifers

Abstract The objective was to evaluate the effect of inclusion rate of pea hay in barley and oat hay when fed to beef cattle. Six ruminally-cannulated heifers (407 ± 38 kg) were used in a 6 × 6 Latin square with a 2 × 3 factorial design and 25-d periods. Treatments included barley or oat hay blended with pea hay to achieve inclusion rates of 0, 15, or 30% (DM basis). Pea inclusion increased DMI (P = 0.03) by 0.75 kg/d, and generally reduced sorting (P ≤ 0.006) against NDF and ADF compared to the cereal-only treatments. Pea inclusion decreased CP digestibility by 2.87% relative to cereal-only treatments, but did not affect the predicted microbial protein supply or nitrogen retention (P ≥ 0.77). Pea inclusion did not affect total ruminal SCFA concentration, but increased the molar proportions of acetate and butyrate and decreased the molar proportion of propionate (P ≤ 0.01). While cereal type did not affect DMI (P = 0.36) or total SCFA concentration (P = 0.61), use of oat hay improved DM digestibility (67.73 vs. 63.22% for oat and barley, respectively; P &amp;lt; 0.001) and increased nitrogen retention (P = 0.03) when compared to barley. Overall, incorporating pea into cereal hay increased DMI, reduced CP digestibility, and altered ruminal fermentation.

PSXII-15 Fiber content in diets for feedlot lambs: ruminal and cecal pH and short chain fatty acids

Abstract Fifty-eight Dorper x Santa Inês ram lambs (initial BW 20.7 ± 1.19 kg and 75 ± 10.83 d old) were assigned to a randomized complete block design to evaluate the effects of fiber content on ruminal and cecal pH and SCFA. The treatments were defined by the fiber (“Coastcross” hay) content in diets (CP: 16,12% ± 0.92): 0F: no forage diet (NDF: 10.4%); 5F: 5% of forage (NDF: 12.1%); 10F: 10% of forage (NDF: 15%); 15F: 15% of forage (NDF: 15.5%); 20F: 20% of forage (NDF: 20.8%) and 25F: 25% of forage (NDF: 23.5%). The experiment lasted 89 days, at the end of the experiment the lambs were slaughtered without fasting. The forage inclusion linear decreased ruminal concentration of propionate (0F: 45.4; 5F: 39.7; 10F: 41.5; 15F: 39.4; 20F: 35.3; 25F: 30.5 mM/100mM; P &amp;lt; 0.01) and total SCFA (0F: 106.6; 5F: 96.4; 10F: 84.2; 15F: 90.6; 20F: 63.0; 25F: 71.2 mM; P = 0.01) and increased the ruminal concentration of acetate (0F: 43.4; 5F: 45.3; 10F: 47.8; 15F: 49.8: 20F: 51.8; 25F: 57.2 mM/100mM; P &amp;lt; 0.01). However, there was a quadratic effect on ruminal pH (0F: 5.61; 5F: 5.66; 10F: 5.98; 15F: 6.27; 20F: 6.05; 25F: 5.74; P = 0.01). There was no effect of fiber levels on cecal concentration of acetate, propionate, butyrate and acetate:propionate ratio. Nevertheless, there was a linear decrease in cecal concentration of valerate (0F: 3.28; 5F: 2.35; 10F: 2.39; 15F: 2.64: 20F: 2.12; 25F: 1.80 mM/100mM; P &amp;lt; 0.01) and total SCFA (0F: 173.9; 5F: 165.7; 10F: 153.2; 15F: 155.2; 20F: 139.9; 25F: 128.9 mM; P &amp;lt; 0.01). Similarly to rumen, cecal pH presented a quadratic response (0F: 5.92; 5F: 6.12; 10F: 6.15; 15F: 6.23; 20F: 6.30; 25F: 5.83; P &amp;lt; 0.01). In conclusion, cecal fermentation was different from rumen fermentation. However, pH variation was similar, with higher values for 15F diet.

PSXII-19 Feed additives association for the rumen fermentation manipulation and reduce production methane in Nellore cattle

Abstract Aimed to evaluate the association between essential oils (Next Enhance 300®), exogenous enzymes (Allzyme SSF®), and monensin for the rumen fermentation manipulation, especially reduction of rumen methane in Nellore cattle. Eight cows weighing 480 ± 55 kg and cannulated in the rumen were used in two 4x4 Latin squares, into a 2x2x2 factorial arrangement, which one of the factors being analyzed by the squared effect. The animals fed the same basal diet using a corn silage: concentrate ratio of 60:40. The factors used were: 300 mg/animal/d, 10 g/animal/d, 300 mg/animal/d for essential oils, exogenous enzyme, and monensin, respectively. Each experimental period lasted 26 days. Sixteen days were used for adaptation to the experimental diets, while from D16 to D21 for evaluation of DMI and D22 to evaluate rumen fermentation (0, 3, 6, 9, and 12h after feeding) through the ex situ technique, which consists of incubating ruminal content in conditions similar to those of the rumen allowing the measurement of rumen production (SCFA and methane). Differences were considered significant when P-value was below 0,05 through analysis of variance using mixed procedure. No significant difference (P &amp;gt; 0.05) was observed for DMI Monensin decreased (P &amp;lt; 0.05) the concentration of acetic, butyric and total SCFA. However, the production of acetic acid, propionic acid, total SCFA and acetate:propionate ratio was not altered (P &amp;gt; 0,05). Significant interaction (P &amp;lt; 0.05) between enzyme and monensin was observed for butyric acid production, indicating the enzyme used alone did not alter the production of these acid, but when associated with monensin, a reduction was observed. The enzyme reduced (P &amp;lt; 0.05) methane concentration, and when associated with monensin this reduction was increased. However methane production was not affected (P &amp;gt; 0.05). The feed additives evaluated do not reduce the rumen production of methane, but reduced butyric acid production.