Total Salt Research Articles

Mitigating seawater intrusion in coastal aquifers: Novel approach with treated wastewater injection and groundwater circulation

Seawater intrusion (SWI) is a natural phenomenon that negatively impacts the potability of groundwater and is expected to worsen with rising sea levels due to climate change. Artificial recharge of freshwater is a commonly-used remediation method to mitigate SWI and improve freshwater supply security in affected coastal aquifers. However, limited freshwater availability can limit the effectiveness of this approach. This study proposes a novel mitigation measure, called Inj-GCW, which combines the injection of reclaimed water with the use of groundwater circulation wells (GCW) to enhance the effectiveness of artificial recharge in controlling SWI. GCW is a dual-screened well with isolated screens that extract and inject groundwater into the aquifer. The performance of the proposed measure was quantitatively evaluated using an illustrative simplified unconfined coastal aquifer. Based on the findings, the design parameters were estimated for a field-scale case study of the Nile Delta aquifer (NDA), a large Mediterranean coastal aquifer in Egypt. The study adopts a future scenario that considers Sea-level rise due to climate change and projected population growth by 2100. Results demonstrate that introducing of GCWs into the saltwater wedge, along with injection, effectively retreated the saltwater wedge, due to enhanced velocity, seaward fluxes, and dilution of contamination. Inj-GCW measure led to an 8.9% reduction in SWI and a 5.2% decrease in aquifer salinity compared to the expected intrusion in 2100. Furthermore, the Inj-GCW measure resulted in a 2.2% higher repulsion rate and 0.3% reduction in total salt mass compared to injection alone. The Inj-GCW measure presents a promising solution to SWI challenges in the NDA and other coastal aquifers facing similar issues. The formation of a brackish water bubble at the injection well screen of the GCW and the generated vertical groundwater circulation cells acted as a hydraulic barrier and contributed to the proposed method effectiveness.

Growing in Saltwater: Biotechnological Potential of Novel Methylotuvimicrobium- and Methylomarinum-like Methanotrophic Bacteria.

Methanotrophic bacteria that possess a unique ability of using methane as a sole source of carbon and energy have attracted considerable attention as potential producers of a single-cell protein. So far, this biotechnology implied using freshwater methanotrophs, although many regions of the world have limited freshwater resources. This study aimed at searching for novel methanotrophs capable of fast growth in saltwater comparable in composition with seawater. A methane-oxidizing microbial consortium containing Methylomarinum- and Methylotuvimicrobium-like methanotrophs was enriched from sediment from the river Chernavka (water pH 7.5, total salt content 30 g L-1), a tributary river of the hypersaline Lake Elton, southern Russia. This microbial consortium, designated Ch1, demonstrated stable growth on natural gas in a bioreactor in media with a total salt content of 23 to 35.9 g L-1 at a dilution rate of 0.19-0.21 h-1. The highest biomass yield of 5.8 g cell dry weight (CDW)/L with a protein content of 63% was obtained during continuous cultivation of the consortium Ch1 in a medium with a total salt content of 29 g L-1. Isolation attempts resulted in obtaining a pure culture of methanotrophic bacteria, strain Ch1-1. The 16S rRNA gene sequence of strain Ch1-1 displayed 97.09-97.24% similarity to the corresponding gene fragments of characterized representatives of Methylomarinum vadi, methanotrophs isolated from marine habitats. The genome of strain Ch1-1 was 4.8 Mb in size and encoded 3 rRNA operons, and about 4400 proteins. The genome contained the gene cluster coding for ectoine biosynthesis, which explains the ability of strain Ch1-1 to tolerate high salt concentration.

Mechanical and Physiochemical Properties of Central Marshes Bed Soils – Southern Iraq

The central marshes are one of the most important wetlands/ecosystems in the southern area of Iraq. This study evaluates the bed soil's mechanical, physical, and chemical properties at certain southern Iraqi central marshes sites. This was conducted to investigate their types and suitability for enhancing the agricultural reality of most field crops and for construction purposes. Soil samples were collected from 15 sites at 10-100 cm depth. Hence, numerous parameters were determined: index properties, unconfined compressive strength, direct shear strength, consolidation, texture, and sieve analysis, water content, specific gravity, dry density, permeability, pH, total soluble salts (TSS), organic materials (OM) and total sulfate content SO3. Results of this study showed that the bed soil of the Central Marshes is classified as clay-silt soil and suitable for planting different crops. Furthermore, Analysis of the results may have a role in the design and planning of upcoming projects, such as the construction of buildings and agriculture, that would have to enlighten the decision maker.

Investigation of Stability and Underlying Mechanism of Unstable Subgrades Loess Modified by Carbide Slag in Road Construction Projection

The repurposing of carbide slag (CS) coupled with the advancement of eco-friendly engineering methodologies promises a novel approach to addressing the technical challenges inherent in loess refinement. This inquiry delves into the feasibility of employing CS as an economically viable and ecologically sustainable remedy to amplify the engineering attributes of loess within the context of optimal preservation. In this investigation, assorted quantities of CS, spanning from 1% to 6%, were infused into the loess. The efficacy of CS as an additive was ascertained through a comprehensive array of tests administered across varied curing durations (0, 5, 10, 20, and 30 days), concentrating on its influence over the soil’s mechanical attributes. The study conducted various tests such as dual hydrometer, Attberg limit, specific gravity, compaction, unconfined compressive strength (UCS), consolidation, physico-chemical properties (pH, conductivity), and chemical analysis (sodium percentage and sodium adsorption rate). The study demonstrated that the incorporation of CS into loess resulted in an increase in hydraulic conductivity, UCS, and optimum water content while reducing maximum dry density, plasticity, and compressibility. Specifically, the application of 6% CS resulted in a significant 6.7-fold increase in UCS from 226.8 (kPa) to 1508.8 (kPa) over a 30-day curing period. It was also observed that the addition of CS and curing period resulted in a decrease in soil pH from 8.5 to 7.5 and an increase in electrical conductivity (EC) from 250 to 490 μs/cm. When the CS concentration was increased to 6%, the amount of Na+ ions, the total soluble salts, and the percentage of Na in the loess decreased. The phenomenon can be ascribed to the replacement of Ca2+ for Na+, leading to a more slender, diffuse double layer and heightened stability. The application of calcium silicate in loess subgrades enhances their stability and potency, concurrently providing an ecologically sound waste management resolution. Consequently, it emerges as a profoundly viable choice for ameliorating loess within the realm of the construction sector.

Diffusion of tritium, technetium, cesium, and uranium from phosphate glass leachates in pore solution of clay materials

The through diffusion method at room temperature was used to study the migration of radionuclides (3H, 99Tc, 137Cs, 233U) in compacted samples of clay materials during pore diffusion from a model leachate of the RW phosphate matrix with a total salt content of about 400 mg/L. Based on the results of the experiments, the effective diffusion coeffcients and coeffcients of the sorption distribution of radionuclides for the studied barrier clay materials were determined. Regularities of the diffusion transfer of tritium, cesium and uranium depending on the structure, mineral composition of clay materials and the content of radionuclides in the pore solution are revealed. Numerical modeles are proposed for calculating the effective pore di usion coeffcients of these radionuclides. There are still insuffcient data to analyze the factors affecting the diffusion of technetium.

Combined Application of Organic Fertilizer with Microbial Inoculum Improved Aggregate Formation and Salt Leaching in a Secondary Salinized Soil.

Greenhouse vegetable production provides significant quantities of vegetables throughout the year and improves farmers' income. However, over-fertilization with mineral fertilizer causes soil secondary salinization and decreases the stability of the soil structure. To improve aggregate formation and decrease salt accumulation in the soil profile, bio-organic fertilizers (Protaetia brevitarsis larvae frass with Bacillus amyloliticus and/or Trichoderma harziensis) were applied to partially substitute mineral fertilizer in a salinized vegetable soil. Soil nutrient condition, aggregate stability, and salt movement in the soil profile were measured in a greenhouse double-cucumber system. The results showed that soil organic matter (SOM), total nitrogen (TN), and available phosphorus (AP) increased significantly under bio-organic fertilizer treatments compared with control. Soil electrical conductivity (EC) and total salt content (TSC) decreased by 15.74-24.20% and 19.15-29.05%, respectively, with bio-organic fertilizers (p < 0.05). Cl-, NO3-, and SO42- content under double inoculation with B. amyloliticus and T. harziensis reduced by 31.19%, 26.30%, and 53.11%, respectively, compared to CK (p < 0.05). In addition, double inoculation was more efficient in reducing nitrate content in the soil profile than single inoculation. Soil microaggregates of 0.25-0.053 mm increased by 75.87-78.51% with bio-fertilizers compared with control, and double inoculation was the best for aggregate formation. In conclusion, the inoculation of plant-growth-promoting and salt-tolerant microorganisms with high humic acid larvae frass can alleviate salinization in vegetable soil, enhance soil nutrient content, and improve the soil structure.

Natrinema salsiterrestre sp. nov., an extremely halophilic archaeon isolated from a hypersaline soil.

An extremely halophilic archaeal strain, designated S1CR25-10T, was isolated from hypersaline soil sampled in the Odiel Saltmarshes Natural Area in Southwestern Spain (Huelva) and subjected to a polyphasic taxonomic characterization. The cells were Gram-stain-negative, motile and their colonies were pink-pigmented. It was a strictly aerobic haloarchaeon that could grow at 25-55 °C (optimum, 37 °C), at pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 12-30 % (w/v) total salts (optimum, 20-25 %, w/v). The phylogenetic analysis based on the comparison of the 16S rRNA gene sequences revealed that strain S1CR25-10T belongs to the genus Natrinema, with 98.9 % similarity to Natrinema salinisoli SLN56T. In addition, the values of orthologous average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity were below the threshold limits accepted for prokaryotic species delineation, with N. salinisoli SLN56T showing the highest relatedness values (92.6 % and 48.4 %, respectively). The major polar lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a glycolipid chromatographically identical to sulfated diglycosyl diether. The DNA G+C content of the isolate was 63.8 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic characterization and the whole genome results, strain S1CR25-10T represents a new species within the genus Natrinema, for which the name Natrinema salsiterrestre sp. nov., with type strain S1CR25-10T (=CECT 30623T=CCM 9251T), is proposed.

Response of soil microbial community diversity to continuous cucumber cropping in facilities along the Yellow River irrigation area.

Cucumber is an important cash crop; however, continuous cropping obstacles readily occur within the intensive production processes of facility horticulture. This study aimed to determine the effects of continuous cropping on soil quality and the microbial community in the rhizosphere soil of cucumbers. Rhizosphere soil of cucumber planted continuously for 4, 8, and 12 years was investigated, and soil that was not continuously planted was used as the control. Soil physicochemical properties, enzyme activity, microbial diversity, and richness were determined. The results showed that with the increase in continuous cropping years (0, 4, 8, and 12 years), soil total salt content continuously increased, while the pH value significantly decreased. Compared with the control, soil organic matter, alkali-hydrolyzed nitrogen, available phosphorus, available potassium, and nitrate nitrogen contents increased significantly after 4 and 8 years of continuous cropping. Spearman correlation analysis showed that pH was negatively correlated with sucrase or sucrose and available phosphorus was positively correlated with alkaline phosphatase. Compared with the control, the diversity and abundance of bacterial and fungal communities in cucumber rhizosphere soil decreased after 4 and 12 years of continuous cropping. Continuous cropping led to a significant increase in the richness of the dominant phylum of cucumber rhizosphere soil. Principal coordinates analysis showed that, compared with the control, the soil microbial community structure was significantly separated after 4, 8, and 12 years of continuous cropping, and the microbial community structure was most similar after 4 and 8 years of continuous cropping. In addition, redundancy analysis showed that pH was the main driver of soil microbial dominance. In conclusion, continuous cropping of cucumber along the Yellow River irrigation area has led to the deterioration of soil nutrients and microbial communities in that region. This experiment provides a theoretical foundation for addressing the challenges associated with continuous cropping in cucumber cultivation.

Analysis of Water and Salt Spatio-Temporal Distribution along Irrigation Canals in Ningxia Yellow River Irrigation Area, China

The large amount of salinized soil in the Yellow River irrigation area is a threat to the sustainable development of agriculture, and in order to efficiently control the soil salinization trend, it is necessary to research water and salt variations and the distribution of soil in the area. In this study, soil salinization along two typical canals (West Main Canal and Tanglai Canal) in the Ningxia Yellow River irrigation area in China is investigated using data regarding water content, salt ions and pH from soil samples collected at 165 points along these canals. At each of these points, soil samples from various layers were collected for measurement from August 2019 to December 2020. Various methods, such as Pearson’s correlation analysis and Kriging interpolation, were employed to obtain the temporal and spatial distributions of water content, pH value and salt ions from the soil samples. It was found that the mean total salt value is 2.75 g/kg, which indicates that the soil in the study area is moderately salinized soil. The average value of soil pH is 8.5, indicating that the soil in this area is alkaline. Furthermore, it is evident that the coefficient of variation for certain soil ions, including HCO3−, Cl−, Ca2+, Na+ and K+, is greater than one, which indicates that their spatial distribution in the study area is severely uneven. Moreover, the soil salt content in the study area gradually increases from the southwest to the northeast, in the flow direction of the West Main Canal and the Tanglai Canal. The water content distribution along the two canals also exhibits notable non-uniformity, displaying a pattern higher in the north and east and less in the south and west. Moreover, the annual distribution of total salt content in the surface layer demonstrates an initial increase followed by a decrease, with the peak value typically occurring in August or September. The research results have an important significance on agriculture in the Ningxia Yellow River irrigation area.

Indirect Prediction of Salt Affected Soil Indicator Properties through Habitat Types of a Natural Saline Grassland Using Unmanned Aerial Vehicle Imagery

Salt meadows, protected within National Parks, cannot be directly surveyed, yet understanding their soil condition is crucial. Our study indirectly estimates soil parameters (Total Salt Content (TSC), Na, and pH) related to salinization/sodification/alkalinization using spectral indices and UAV survey-derived elevation model, focusing on continental lowland salt meadows. A vegetation map was created using 16 spectral indices and a Digital Elevation Model calculated from RGB orthophotos using photogrammetry. Field observations helped define habitat types based on the General National Habitat Classification System (Hungary), and quadrats with complete coverage of specific plant species were identified. Machine learning was employed on 84 training quadrats to develop a prediction algorithm for vegetation patterns. Five saline habitat types, representing variations in soil properties and topography, were identified. Spectral and topomorphometric indices derived from UAV were key to the spatial prediction of soil properties, employing random forest and co-kriging methods. TSC, Na, and pH data served as indicators of salt-affected soils (SAS), and thematic maps were generated for each indicator (57 samples). Overlapping with the vegetation map, the probability range of estimated SAS indicator values was determined. Consequently, a model-based estimation of soil pH, TSC, and Na conditions is provided for habitat types without disturbing protected areas.

Status of Sulphur, Zinc and Boron in Onion Growing Soils of Dharwad Taluk, Karnataka, India

Survey was undertaken during rabi 2021-22, to study the sulphur, zinc and boron status in onion growing fields in selected villages of Dharwad taluk, Karnataka. One hundred surface soil samples were collected from selected ten villages of Dharwad taluk using GPS. The results revealed that soils were neutral to alkaline in reaction, low in total soluble salts, low to high organic carbon content, low in available nitrogen, medium in available phosphorus, potassium and sulphur and 49.00 and 77.00 per cent of the soils were deficient in DTPA extractable zinc and hot water soluble boron, respectively.

Responses of the structure and function of microbes in Yellow River Estuary sediments to different levels of mercury

The health and stability of the estuary of the Yellow River ecosystem have come under increasing pressure from land-based inputs of heavy metals. While it is known that heavy metals affect the function and health of the microbial community, there remains little knowledge on the responses of the microbial community to heavy metals, particularly highly toxic mercury. The research aimed to characterize the responses of the sediment microbial community of the estuary of the Yellow River to different levels of mercury stress. Estuary sediment samples were collected for microbial community analysis, measurement of mercury [including total mercury (THg) and methylmercury (MeHg)], and measurement of other physicochemical factors, including pH, total organic carbon (TOC), sulfide, iron ratio (Fe3+/Fe2+), ammonium salt (NH4+), and biochemical oxygen demand (BOD). The application of 16S rRNA sequencing identified 60 phyla of bacteria, dominated by Proteobacteria, Firmicutes, and Bacteroidetes. Stations with higher THg or MeHg and lower microbial abundance and diversity were generally distributed further outside of the estuary. Besides mercury, the measured physicochemical factors had impacts on microbial diversities and distribution. Metagenomics assessment of three stations, representative of low, moderate, and high mercury concentrations and measured physicochemical factors, revealed the abundances and functions of predicted genes. The most abundant genes regulating the metabolic pathways were categorized as metabolic, environmental information processing, and genetic information processing, genes. At stations with high levels of mercury, the dominant genes were related to energy metabolism, signal transport, and membrane transport. Functional genes with a mercury-resistance function were generally in the mer system (merA, merC, merT, merR), alkylmercury lyase, and metal-transporting ATPase. These results offer insight into the microbial community structure of the sediments in the Yellow River Estuary and the microbial function of mercury resistance under mercury stress.

Biological Characterization of Pseudomonas fluorescens Phage Pf17397_F_PD1 and Its Application in Food Preservation

In order to explore the application prospects of phages for controlling bacterial contamination, a lytic phage Pf17397_F_PD1 (Later abbreviated as PD1) was isolated from fish guts using Pseudomonas fluorescens ATCC 17397 as the host bacterium. The phage displayed short latency (18 min), long lysis period (212 min), and high lysis volume (1.47 × 102 PFU/each cell). It displayed wide temperature (30–70°C) and pH (4–11) tolerance. Genomic comparison revealed a maximum sequence identity of 48.65% between phage PD1 and other identified phages, indicating that PD1 was a new phage. The phage PD1 significantly inhibited the growth of P. fluorescens in milk and grass carp at 4°C and 25°C. Compared to the negative control, bacterial levels in milk stored at 25°C for 48 h were reduced by 2.71 log CFU/mL and 2.84 log CFU/mL at the multiplicity of infection (MOI) of 100 and 1,000, respectively. In contrast, when grass carp were stored at 25°C for 24 h, the bacterial load was reduced by 1.28 log CFU/g and 2.64 log CFU/g compared to the control (MOI of 100 and 1,000). When the phage was applied for preservation of grass carp blocks, total volatile salt nitrogen (TVB-N) values of phage-treated samples increased by 6.8 mg/100 g and 7.5 mg/100 g at MOI of 100 and 1,000, respectively, after 7 days of storage, which was significantly lower than that of the control group (15.83 mg/100 g). This study showed that phage PD1 was a good natural biological antimicrobial agent against P. fluorescens ATCC 17397.

Effects of the combined application of biochar and humic substances on the improvement of saline cropland in the Yellow River Delta of China

AbstractSoil salinization has become a global environmental problem with the potential to cause serious land degradation. Thus, improving salt‐affected soils and enhancing the nutrient utilization efficiency of saline farmlands through scientific and effective measures are urgent scientific problems. In this study, a 2‐year field experiment was conducted to evaluate the effects of different soil amendments and their combinations on the dynamics of soil salinity, soil nutrients, and crop yield in a moderately salinized soil (EC1:5 of 0.78 dS m−1 and total salt content of 3.06 g kg−1). Nine soil treatments were implemented: biochar (B), lignocellulose (L), humic acid (H), fulvic acid (F), biochar + humic acid (B + H), biochar + fulvic acid (B + F), lignocellulose + humic acid (L + H), lignocellulose + fulvic acid (L + F), and control (CK). The results demonstrated that the application of F led to a certain reduction on the contents of soil salt, Na+, and Cl− among the soils amended with a single amendment application. The combined application of B and F or H could increase the soil desalination rate and increase the mineral N and Olsen‐P concentrations at a soil depth of 0–20 cm, which improved the grain yield, biological yield, N recovery efficiency (REN), N agronomic efficiency (AEN), N partial factor productivity (PFPN), P agronomic efficiency (AEP), and P partial factor productivity (PFPP). Although the single application of L led to decreases in the grain yield, biological yield, REN, AEN, PFPN, REP, AEP, PFPP, and P physiological efficiency (PEP), the combined application of L and F had superior effects on enhancing the winter wheat grain yield and accumulative nutrient use efficiency. In conclusion, the combined application of B and F or H is recommended to ameliorate salt‐affected soils and improve nutrient availability.

Separation of organic ions from aqueous solutions by membrane capacitive deionization

Persistent and mobile organic chemicals are a growing concern for the management of water resources. Here, we propose membrane capacitive deionization (MCDI) for effective separation and selective recovery of these chemicals. Using a simple MCDI process, with activated-carbon-cloth electrodes, we experimentally demonstrate the feasibility of MCDI for adsorbing permanently charged ionic liquids (ILs) and ionogenic chemicals (IOCs) such as perfluorooctanoic acid (PFOA) and diclofenac. The total salt adsorption capacity (SAC) of ILs and IOCs is between 5.12 and 8.83 µmol/g and is strongly dependent on the symmetry of cation and anion mobilities, as represented by their diffusion coefficients. The system has a low energy consumption of 0.031 kWh/m3, with a productivity of 83.33 L/h/m2. As an example of pharmaceuticals in wastewater we examined diclofenac solution and show a total SAC of 3.03 µmol/g. These results demonstrate the potential of this deionization process for the cleanup of industrial or medical wastewater.

A CASE STUDY ON THE POSSIBILITY OF SOIL MELIORATIVE STATE ASSESSMENT BY REMOTE SENSING DATA

The paper presents the results of the pilot study on the relationship between spatial bare-soil normalized difference vegetation index and content of salts in the dark-chestnut soil of the South of Ukraine. The study is based on the results of soil analyses, conducted in 2016 within the framework of sweet corn cultivation technology investigation at the Agricultural Cooperative Farm of Bilozersky district, Kherson oblast. The results of laboratory analyses were connected to bare-soil values of the spatial vegetation index, obtained at OneSoil platform. The relationship was estimated through rank correlation and polynomial regression analysis. As a result, very strong relationship was established between bare-soil vegetation index and toxic salts content in the soil, while moderate inter-connection was found out between the index and total salts content in the soil. The second-grade polynomial models, developed in the intercourse of regression analysis, proved to have very good fitting quality and accuracy of the salts content prediction, with the mean absolute percentage error 2.93-4.27%. Thus, bare-soil normalized difference vegetation index is suitable and prospective for meliorative surveys of the dark-chestnut soils of the South of Ukraine.

Microbiota-Liver-Bile Salts Axis, a Novel Mechanism Involved in the Contrasting Effects of Sodium Selenite and Selenium-Nanoparticle Supplementation on Adipose Tissue Development in Adolescent Rats.

Adolescence is a period during which body composition changes deeply. Selenium (Se) is an excellent antioxidant trace element related to cell growth and endocrine function. In adolescent rats, low Se supplementation affects adipocyte development differently depending on its form of administration (selenite or Se nanoparticles (SeNPs). Despite this effect being related to oxidative, insulin-signaling and autophagy processes, the whole mechanism is not elucidated. The microbiota-liver-bile salts secretion axis is related to lipid homeostasis and adipose tissue development. Therefore, the colonic microbiota and total bile salts homeostasis were explored in four experimental groups of male adolescent rats: control, low-sodium selenite supplementation, low SeNP supplementation and moderate SeNPs supplementation. SeNPs were obtained by reducing Se tetrachloride in the presence of ascorbic acid. Supplementation was received orally through water intake; low-Se rats received twice more Se than control animals and moderate-Se rats tenfold more. Supplementation with low doses of Se clearly affected anaerobic colonic microbiota profile and bile salts homeostasis. However, these effects were different depending on the Se administration form. Selenite supplementation primarily affected liver by decreasing farnesoid X receptor hepatic function, leading to the accumulation of hepatic bile salts together to increase in the ratio Firmicutes/Bacteroidetes and glucagon-like peptide-1 (GLP-1) secretion. In contrast, low SeNP levels mainly affected microbiota, moving them towards a more prominent Gram-negative profile in which the relative abundance of Akkermansia and Muribaculaceae was clearly enhanced and the Firmicutes/Bacteroidetes ratio decreased. This bacterial profile is directly related to lower adipose tissue mass. Moreover, low SeNP administration did not modify bile salts pool in serum circulation. In addition, specific gut microbiota was regulated upon administration of low levels of Se in the forms of selenite or SeNPs, which are properly discussed. On its side, moderate-SeNPs administration led to great dysbiosis and enhanced the abundance of pathogenic bacteria, being considered toxic. These results strongly correlate with the deep change in adipose mass previously found in these animals, indicating that the microbiota-liver-bile salts axis is also mechanistically involved in these changes.

Soil salinity estimation in Shule River Basin using support vector regression model

AbstractSoil salinization is a progressive degradation process that spreads globally and leads to a decline in soil fertility. Assessing the scale of salinization is crucial for sustainable agricultural development and saline‐land rehabilitation. In this study, we proposed a support vector regression spatial (SVR‐S) model that utilizes spatial dependence information to predict soil salinity over the irrigation area of the Shule River Basin in northwestern China. To investigate the performance of the SVR‐S model, 50 soil samples were collected in the field. Semivariograms of soil salinity (measured as total salt content and ion concentrations) were constructed to measure spatial dependence. The SVR‐S model was compared with the original SVR model and the geographically weighted regression (GWR) model regarding the salinity prediction ability. The soil salinity in the experimental area demonstrated a strong spatial dependence pattern. The SVR‐S model delivered a better performance than the SVR‐O and GWR. SVR‐S showed a correlation coefficient R of 0.87 and a root mean square error (RMSE) of 1.83%, while the performance of SVR‐O (R = 0.75, RMSE = 3.32%) and GWR (R = 0.73, RMSE = 3.47%) was comparatively poor. Topographic indices integrating spatial information contributed the most to the estimation of salinity in the study area. This study provides a new approach to integrating spatial information for accurate soil salinity mapping.

Size and Composition Control of Magnetic Nanoparticles

Magnetite nanoparticles are a type of magnetic nanoparticle (MNP) that are inexpensive to make and can be formed in different shapes and sizes. MNPs can be used in DNA/RNA purification, drug delivery, and other biomedical applications. The commonly employed solvothermal route to make these MNPs gives the most uniform products with the smallest size distribution, but there is a need to identify easily controlled changes to reaction parameters that can reliably tune the size and composition of the MNPs made by this method. In this work we report simple and reliable methods whereby adjusting the total water content and basic salt concentration affords size and composition control, respectively. We employ synchrotron X-ray absorption measurements to prove our method offers monotonic control over MNP composition from maghemite-rich (γ-Fe2O3) to the more magnetic magnetite (Fe3O4). This demonstrates a simple, general method to produce particles with high magnetite concentration and the desired size.

Nature-inspired synthetic oligourea foldamer channels allow water transport with high salt rejection

•Synthetic, self-assembling, helical oligourea foldamers form artificial water channels •ssNMR, MD simulation, and cryo-EM data show proper insertion of foldamers into liposomes •Water-selective channels are formed by oligomerization of the amphipathic foldamers •4.8 Å hydrophilic pore is present in X-ray crystal structure of foldamer H2OC1 Biomimetic membranes incorporating artificial water channels (AWCs) are being developed for industrial water purification. Designing AWCs to achieve high water permeation with salt rejection is a challenge. We designed and synthesized oligourea foldamers, which form predictable helical structures that can be used to create biomimetic porin-like architectures. Two of these foldamers (H2OC1 and H2OC2) allow superior water permeability and almost total salt rejection across lipid membranes. Solid-state NMR, cryo-EM, and molecular dynamics analyses suggest proper insertion of foldamers into lipid vesicles. The H2OC1 crystal structure shows hydrophilic pores of diameters 4.8 and 6.4 Å. The oligourea helices pack together by hydrophobic and salt bridge interactions to build two channel-like assemblies. Besides their proteolytic stability and microbial resistance, the sequence of foldamers can be tailored to regulate selectivity. The ease of designing, synthesizing, and purifying oligourea foldamers is an added advantage. Our findings can help to develop novel AWCs for water purification applications. Biomimetic membranes incorporating artificial water channels (AWCs) are being developed for industrial water purification. Designing AWCs to achieve high water permeation with salt rejection is a challenge. We designed and synthesized oligourea foldamers, which form predictable helical structures that can be used to create biomimetic porin-like architectures. Two of these foldamers (H2OC1 and H2OC2) allow superior water permeability and almost total salt rejection across lipid membranes. Solid-state NMR, cryo-EM, and molecular dynamics analyses suggest proper insertion of foldamers into lipid vesicles. The H2OC1 crystal structure shows hydrophilic pores of diameters 4.8 and 6.4 Å. The oligourea helices pack together by hydrophobic and salt bridge interactions to build two channel-like assemblies. Besides their proteolytic stability and microbial resistance, the sequence of foldamers can be tailored to regulate selectivity. The ease of designing, synthesizing, and purifying oligourea foldamers is an added advantage. Our findings can help to develop novel AWCs for water purification applications.