Total Protein Amino Acid Research Articles

NITROGEN METABOLISM DURING SYNCHRONOUS GROWTH OF CHLORELLA PYRENOIDOSA. I. PROTEIN AMINO ACID DISTRIBUTION.

AbstractTotal cellular‐N, acid soluble‐N, lipid plus chlorophyll‐N, and the levels of the individual amino acids were measured during synchronous growth of a high temperature strain, 7‐11‐05, of Chlorella pyrenoidosa. Total cellular‐N increased exponentially except for a small but reproducible deviation from log‐linearity which occurred at approximately the middle of the 14 hour synchronous growth cycle. Although the level of acid soluble‐N (as % of total cellular‐N) exhibited definite periodism during synchronous growth, the amount of nitrogen in this pool was small (approximately 4–8%). Lipid‐ plus chlorophyll‐N showed only slight fluctuations during cellular development; however, the trend followed that previously observed for phospholipid‐P. The levels of the protein amino acids were expressed on a mole‐percentage basis of the total protein amino acids. The level of most of the protein amino acids remained essentially constant during the course of synchronous growth. The levels of the basic and acidic amino acids as well as alanine exhibited more significant periodism than the other protein amino acids. The total protein amino acid‐N level comprised approximately 63% of the total cellular‐N throughout cellular development.

鼠らい菌の核酸及びアミノ酸

Murine leprosy bacilli were collected with the Nakamura-Hanks' method from the subcutaneous leproma of rats on the three months after inoculation, and lyophilized. The authors' studies were examined on the ribonucleic acid and amino acid of bacilli. The findings to be described were as follows :1) The ribonucleic acid was extracted with the phenol method and fractionated. The r-RNA, represented for the constituent to alternate rapidly, was for the most part and the p-A-RNA (s-RNA ?), persumably taking part to protein synthesis, was for scanty part : thus, the p-A : r value was such a low value as 1: 5. From these findings it may be suggested the murine leprosy bacilli were standing in rather resting phase concerning to the cell growth.2) The total free amino acids of leprosy bacilli, the source of protein biosynthesis, was such a small quantity as to be less than two times of the total protein-amino acids, and in the former the amino acid such as glycine, arginine, leucine, methionine and cystine were less than in the latter.The authors' idea from the evidence at hands seems to suggest the slinghtness of the ability of protein biosynthesis of murine leprosy bacilli, namely, it may be called almost impossible of rapid multiplication of murine leprosy bacilli.