Total Phosphate Research Articles

Evaluation of Various Concentrations of Rumen Waste in making Liquid Organic Fertilizer

Slaughterhouses are known as places to produce meat for human consumption. On the other hand, slaughterhouses produce livestock waste, one of which is rumen. The rumen's high nutrient and microbial content provide an excellent opportunity for its use as liquid organic fertilizer for plant growth and development. However, evaluation of rumen addition concentration during the manufacture of liquid organic fertilizer is still scarce and needs to be carried out. Therefore, this research aims to obtain a suitable rumen addition formulation for making liquid organic fertilizer. Rumen waste was varied in 4 concentrations, namely 20%, 40%, 60% and 80%. Supporting ingredients, such as laos, ginger, turmeric, galangal, and rice washing water, are mixed with the rumen, which is then incubated for 15 days to obtain liquid organic fertilizer. Analysis of total nitrogen content, C/N ratio, total phosphate, total potassium, total microbes, and physical characteristics was carried out to evaluate the quality of liquid organic fertilizer. The results showed that the addition of 80% rumen was able to play a role in producing liquid organic fertilizer with high total nitrogen (2.35%), low C/N ratio (6.05), high total phosphate (1.19%), and high total potassium (1.75%). Furthermore, liquid organic fertilizer made with the addition of 80% rumen contains high total microbes (5.5 x 1010 CFU/mL), is bright brown, yellowish in color, and has an earthy aroma. This research indicates that adding 80% rumen can produce good quality liquid organic fertilizer. This information can be the basis for further research regarding the rumen potency as an ingredient of organic fertilizer.

Continuous Straw Returning Combined with Nitrogen Application Improve Soil Properties and Yield of Double Cropping Maize in Subtropical Regions

This study aimed to investigate the impact of straw returning (SR) combined with appropriate N application rates on soil properties and maize yield for a double cropping maize system in South China. From 2021 to 2022, a two-year field experiment was conducted (the perennial orientation study began in 2018) with two nitrogen application rates, 0 kg ha−1 (N0) and 250 kg ha−1 (N250), under various straw treatments (SR and traditional planting). The findings revealed that SR, along with the nitrogen application of 250 kg ha−1 (N250), increased soil total nitrogen (TN), soil total phosphorous (STP), and the soil total potassium (STK) content besides soil organic carbon (SOC) and labile organic carbon (LOC); similarly, their interaction improved SOC and LOC in the 0–20 cm soil layer. In addition, within the 20–40 cm soil layer, SR and N250 also increased the soil TN, SOC, LOC, STP, and STK content. Notably, these soil properties exhibited a decrease with increasing soil depth. Furthermore, SR and N250 led to improvements in the grain yield and yield component of maize. Combining SR with N250 led to a significant 101.53% increase in SOC content from 2018 to 2022. Our research indicates that implementing N rates of 250 kg ha−1 under SR is an effective method to boost maize grain yield, enhance soil chemical characteristics, and ensure safe and productive maize cultivation.

Nitrogen uptakes dynamics with environmental variables in the southwest Bay of Bengal

Nitrate uptake is an essential nutrient for primary production. A study focused on the surface waters of the Bay of Bengal (BoB), new, regenerated, and total production were estimated from nitrate, ammonia, and urea (nitrogen uptakes). Understanding nitrogen uptake rates in coastal waters, where nutrient limitations can disturb environmental biological productivity, is essential. A detailed study of these uptake rates and metabolic processes is required to develop effective mitigation strategies to prevent further degradation of these ecosystems. Total production ranged between 1.39 and 7.43 mmol N m2 d−1, new production ranged between 0.58 and 2.83 mmol N m2 d−1 and regenerated production ranged between 0.83 and 4.59 mmol N m2 d−1. The study observed a significant negative correlation nitrogen uptake along with pH, sea surface salinity (SSS), and sea surface temperature (SST) was observed in the study. The R2 values for SST were 0.605, 0.619, 0.503, 0.601, and 0.627; for SSS they were 0.688, 0.511, 0.498, 0.579, and 0.644 with nitrogen (Na15NO3), ammonium (15NH4Cl), urea (CO(15NH2)2), regenerated, and total production uptake, respectively. pH was highly correlated with nitrate uptake (R2 = 0.525), had a low correlation with ammonium uptake (R2 = 0.439) and a moderate correlated with urea uptake (R2 = 0.526). A positive relationship of nitrogen uptakes with chlorophyll-a, dissolved oxygen, and dissolved inorganic nitrogen (DIN) were observed. Chlorophyll-a had R2 value of 0.608, 0.126, 0.524, 0.526, 0.578, with Na15NO3, 15NH4Cl, CO(15NH2)2, regenerated and total production uptake respectively. Dissolved oxygen (DO) related to ammonium uptake showed a very poor correlation (R2 = 0.079) but a better correlation with urea (R2 = 0.534). New production uptake rate showed a high positive correlation with DO (R2 = 0.645), whereas regenerative production uptake rates showed a relatively low correlation (R2 = 0.519). The positive relationship between DIN and nitrogen uptake had corresponding R2 value of 0.642, 0.591, 0.558, 0.652 and 0.675 for nitrite, ammonium, urea, regenerated and total production uptakes respectively. Total nitrogen: Total phosphate (TN:TP) showed a positive correlation with ammonium. The TN:TP relationship fit nicely with R2 = 0.576 (nitrate uptake), 0.524 (ammonium uptake), and 0.503 (urea uptake) in the coastal BoB. Hence, by applying statistical analysis, principal component analysis and pearson correlation, the interdependency of the environmental parameters enhancing the new production has been confirmed.

High-accuracy quantitative model for phosphate anions in solution based on absorption spectroscopy and machine learning algorithms

The eutrophication pollution caused by excessive phosphorus seriously affects production and daily life of the people. Accurate and rapid detection of phosphate is crucial for water quality management and cleaner production. Currently, most analytical methods for phosphates involve the addition of multiple chemical reagents and are incapable of simultaneous quantification of total phosphorus (TP) and phosphate anions. The environmental friendliness and analytical performance of the methods need to be improved. Here, we established a green and high-accuracy quantitative model based on absorption spectroscopy and machine learning algorithms, which achieved the analysis of TP, H2PO4− and HPO42−. Based on the pH and absorption spectra of phosphate anions, classification and regression tree (CART), random forest (RF), and extreme gradient boosting (XGBoost) algorithms were used to train the original absorption spectra data, first-order derivative spectra data, and multiple feature parameter data of phosphate solutions with pH 4.7–10.0. By comparing the R2, root mean square error (RMSE) and mean absolute error (MAE) evaluation indicators, it was found that the phosphate anions quantitative model established using the CART based on the feature parameters of 187 nm performed the best, R2 > 0.9999, RMSE <3.4 × 10−6, MAE <8.1 × 10−7. The quantitative ranges of TP, H2PO4−, and HPO42− were 3.98 × 10−4—9.33 × 10−4 mol/L, 5.98 × 10−6—4.20 × 10−4 mol/L and 1.53 × 10−6—7.43 × 10−4 mol/L, respectively. Moreover, the quantitative model was optimized based on the spectral characteristics of 14 interfering ions, to improve the model analytical performance for unknown samples. The quantitative model developed in the study achieves accurate analysis of TP, H2PO4− and HPO42−, which is in line with green chemistry concept, and has the potential for prediction and early warning of water environment quality.

Green solutions for antibiotic pollution: Assessing the phytoremediation potential of aquatic macrophytes in wastewater treatment plants

We compared the ability of one emergent (Sagittaria montevidensis), two floating (Salvinia minima and Lemna gibba), and one heterophyllous species (Myriophyllum aquaticum) to simultaneously remove sulfamethoxazole, sulfadiazine, ciprofloxacin, enrofloxacin, norfloxacin, levofloxacin, oxytetracycline, tetracycline, doxycycline, azithromycin, amoxicillin, and meropenem from wastewater in a mesocosm-scale constructed wetland over 28 days. Antibiotic concentrations in plants and effluent were analyzed using an LC-MS/MS to assess the removal rates and phytoremediation capacities. M. aquaticum did not effectively mitigate contamination due to poor tolerance and survival in effluent conditions. S. minima and L. gibba demonstrated superior efficiency, reducing the antibiotic concentrations to undetectable levels within 14 days, while S. montevidensis achieved this result by day 28. Floating macrophytes emerge as the preferable choice for remediation of antibiotics compared to emergent and heterophyllous species. Antibiotics were detected in plant tissues at concentrations ranging from 0.32 to 29.32 ng g−1 fresh weight, highlighting macrophytes' ability to uptake and accumulate these contaminants. Conversely, non-planted systems exhibited a maximum removal rate of 65%, underscoring the persistence of these molecules in natural environments, even after the entire experimental period. Additionally, macrophytes improved effluent quality regardless of species by reducing total soluble solids and phosphate concentrations and mitigating ecotoxicological effects. This study underscores the potential of using macrophytes in wastewater treatment plants to enhance overall efficiency and prevent environmental contamination by antibiotics, thereby mitigating the harmful impact on biota and antibiotic resistance. Selecting appropriate plant species is crucial for successful phytoremediation in constructed wetlands, and actual implementation is essential to validate their effectiveness and practical applicability.

A simple method to overcome paraproteinemic interferences in chemistry and immunoassays

Abstract Background Interferences on chemistry and immunoassay results due to paraproteinemia may lead to erroneous diagnoses and treatment. Such interferences are difficult to recognize and even more difficult to deal with. This report describes 1 such case where multiple measurands were affected and how the interferant was overcome. Case Report Paraproteins present in an immunoglobulin (Ig)G-lambda multiple myeloma specimen interfered with results of total bilirubin, direct bilirubin, inorganic phosphate, iron, ferritin, and total thyroxine measured on 3 platforms: AU5800, Alinity ci, and cobas pure. Repeat testing upon dilution with normal saline or deproteinization by polyethylene glycol precipitation gave unsatisfactory results on some or all the affected measurands. Repeat testing after dilution of the interferant serum with a healthy serum corrected the anomalous results for all the affected measurands. Conclusion Dilution of paraproteinemic serum with a healthy serum of known concentrations appears to be the most suitable method to negate the effects of paraproteinemic interferences.

Microbes and nutrient shift in a Closed Aquatic Ecosystem (CAES) during four weeks of operation

A Closed Aquatic Ecosystem (CAES) housed an aquatic plant Ceratophyllum demersum, zebrafish (Danio rerio), and microbes that were simultaneously obtained with the zebrafish, and it was used to study the operation of the ecosystem. The results indicated that the CAES can operate steadily for about 4 weeks. The dissolved oxygen (DO), pH, and conductivity values of the ecosystem regularly oscillated, while the total nitrogen of the water decreased and the total phosphate slightly increased. Additionally, the chemical oxygen demand (COD, a measure of organic compounds) of the water after the experiment increased to 39 times more than that of the water before the experiment. The meta-genomic data showed that the number of genera decreased by 38 % and the top 10 most abundant genera were almost completely different before and after the experiment, which demonstrated a great shift in the microbes during the operation process. These results suggested that although the CAES operated steadily during the 28-day experiment, there were more organic materials and less nitrogen in the water by the end of the experiment, which may have influenced the structure and operation of the ecosystem. Thus, it is necessary to remove superfluous plant biomass from the CAES and supply nitrogen to keep the ecosystem stable.

Emerging pollutant in surface water bodies: a review on monitoring, analysis, mitigation measures and removal technologies of micro-plastics.

Water bodies play a crucial role in supporting life, maintaining the environment, and preserving the ecology for the people of India. However, in recent decades, human activities have led to various alterations in aquatic environments, resulting in environmental degradation through pollution. The safety of utilizing surface water sources for drinking and other purposes has come under intense scrutiny due to rapid population growth and industrial expansion. Surface water pollution due to micro-plastics (MPs) (plastics < 5mm in size) is one of the emerging pollutants in metropolitan cities of developing countries because of its utmost resilience and synthetic nature. Recent studies on the surface water bodies (river, pond, Lake etc.) portrait the correlation between the MPs level with different parameters of pollution such as specific conductivity, total phosphate, and biological oxygen demand. Fibers represent the predominant form of MPs discovered in surface water bodies, exhibiting fluctuations across seasons. Consequently, present study prioritizes understanding the adaptation, prevalence, attributes, fluctuations, and spatial dispersion of MPs in both sediment and surface water environments. Furthermore, the study aims to identify existing gaps in the current understanding and underscore opportunities for future investigation. From the present study, it has been reported that, the concentration of MPs in the range of 0.2-45.2 items/L at the Xisha Islands in the south China sea, whereas in India it was found in the range of 96 items/L in water samples and 259 items/kg in sediment samples. This would certainly assist the urban planners in achieving sustainable development goals to mitigate the increasing amount of emergent pollutant load.

Spatiotemporal Assessment of Water Pollution for Beira Lake, Sri Lanka

Beira Lake, located in Colombo, Sri Lanka, has suffered severe anthropogenic impacts, with previous restoration attempts failing due to a limited understanding of pollutant dynamics. Aiming to fill this gap, a comprehensive study was conducted during dry and wet seasons to assess the spatiotemporal water pollution of Beira Lake, employing key physicochemical parameters, numerical indices, and remote sensing analysis. The water pollution index (WPI) results categorize Beira Lake as highly polluted, with WPI values ranging from 2.38 ± 0.92 in the wet season to 2.53 ± 1.32 in the dry season. Comparatively higher COD levels recorded in the Beira Lake network, especially for Gangarama Lake show significant pollution levels during both the dry and wet seasons, e.g., the highest COD levels, at 306.40 mg/L, were observed during the wet season. The Trophic State Index (TSI) results indicate eutrophic and hypereutrophic conditions in Beira Lake, which are particularly pronounced during the wet season. The heavy metal pollution index (HPI) results suggest elevated heavy metal concentrations in Beira Lake, especially in the wet season. Combined with field investigation results, a remote sensing data analysis between 2016 and 2023 reveals significant improvements in water transparency, suggesting positive effects of recent management interventions. Parameters demanding attention include COD, nitrate, and total phosphate levels due to their consistent exceedance of permissible limits. The PCA results of indices correlations between wet and dry seasons offer valuable insights into the complex dynamics of Beira Lake’s water quality. The study makes recommendations for restoring Beira Lake, including stringent pollution controls, regular dredging, green infrastructure implementation, implementing new rules and regulations, and community engagement.

Conservation tillage: a way to improve yield and soil properties and decrease global warming potential in spring wheat agroecosystems.

Climate change is one of the main challenges, and it poses a tough challenge to the agriculture industry globally. Additionally, greenhouse gas (GHG) emissions are the main contributor to climate change; however, croplands are a prominent source of GHG emissions. Yet this complex challenge can be mitigated through climate-smart agricultural practices. Conservation tillage is commonly known to preserve soil and mitigate environmental change by reducing GHG emissions. Nonetheless, there is still a paucity of information on the influences of conservation tillage on wheat yield, soil properties, and GHG flux, particularly in the semi-arid Dingxi belt. Hence, in order to fill this gap, different tillage systems, namely conventional tillage (CT) control, straw incorporation with conventional tillage (CTS), no-tillage (NT), and stubble return with no-tillage (NTS), were laid at Dingxi, Gansu province of China, under a randomized complete block design with three replications to examine their impacts on yield, soil properties, and GHG fluxes. Results depicted that different conservative tillage systems (CTS, NTS, and NT) significantly (p < 0.05) increased the plant height, number of spikes per plant, seed number per meter square, root yield, aboveground biomass yield, thousand-grain weight, grain yield, and dry matter yield compared with CT. Moreover, these conservation tillage systems notably improved the soil properties (soil gravimetric water content, water-filled pore space, water storage, porosity, aggregates, saturated hydraulic conductivity, organic carbon, light fraction organic carbon, carbon storage, microbial biomass carbon, total nitrogen, available nitrogen storage, microbial biomass nitrogen, total phosphorous, available phosphorous, total potassium, available potassium, microbial counts, urease, alkaline phosphatase, invertase, cellulase, and catalase) while decreasing the soil temperature and bulk density over CT. However, CTS, NTS, and NT had non-significant effects on ECe, pH, and stoichiometric properties (C:N ratio, C:P ratio, and N:P ratio). Additionally, conservation-based tillage regimes NTS, NT, and CTS significantly (p < 0.05) reduced the emission and net global warming potential of greenhouse gases (carbon dioxide, methane, and nitrous oxide) by 23.44, 19.57, and 16.54%, respectively, and decreased the greenhouse gas intensity by 23.20, 29.96, and 18.72%, respectively, over CT. We conclude that NTS is the best approach to increasing yield, soil and water conservation, resilience, and mitigation of agroecosystem capacity.

Invasive Aquatic Plants as Potential Sustainable Feedstocks for Biochar Production and as an Innovative Approach for Wastewater Treatment

Biochar (BC) is a well-established physical treatment method. The high-cost BC limits their use as adsorbents in wastewater. Thus, deriving BC from cheap and locally available waste materials is needed to develop a feasible waste removal technology. Nowadays, BC technology makes it possible to envision a new strategy to manage invasive plants by converting them into value-added products like BC. Hence, the present study was designed to evaluate the potential utilization of BC as an efficient filter medium made by invasive aquatic plants, Salvinia spp., and Eichhornia spp. A mass of 50 g of prepared activated and nonactivated BC was incorporated in a sand and gravel filter to treat rubber-manufactured wastewater. Wastewater was passed through the filter, and both raw and treated water samples were analyzed for pH, Total Suspended Solids (TSS), Biological Oxygen Demand (BOD5), Chemical Oxygen Demand (COD), Total Kjeldahl Nitrogen (TKN), Ammoniacal-Nitrogen (NH3-N), Electrical Conductivity (EC), Total Dissolved Solids (TDS), Total Phosphates (TP), Nitrate (NO3-N), turbidity and heavy metals (Zinc, Chromium). The control filter was developed only with sand and gravel, excluding BC. Fourier Transform-Infrared Spectroscopy (FT-IR) and Scanning electron microscopy (SEM) were used to analyze BC’s chemical and physical characteristics. A brine shrimp lethality assay was carried out for toxicological evaluation. OH stretching (3,550-3,200 cm−1), C=C aromatic stretching (1400-1660 cm−1), and Phenol-O-H bending (1,300-1,400 cm−1) were recorded in all BC samples that involved the adsorption mechanism. Observed images indicated differences in surface morphology of both activated and nonactivated BC were observed under SEM observation. The study concludes that the filter unit incorporated with activated Eichhornia spp. Gave the best treatment efficiency when compared to filter units incorporated with other activated and nonactivated BC. The toxicity assay revealed 100% mortality in the control setup and raw wastewater but only 60–70% in the nonactivated BC integrated filters. Activated BC-incorporated filters showed no mortalities. Hence, the study’s outcomes suggest a green approach using invasive aquatic plants for sustainable wastewater treatment.

Nutrient Removal Efficiencies in a Bioretention Cell Using Pre-Treated Coconut By-Product as Carbon Source

Abstract In this research, the appropriateness of alkali pre-treatment on coconut by-products was managed by exposing coconut husk and shell to 2M NaOH. The powdered samples were analysed for morphology observation, Fourier Transform Infrared (FTIR), Particle Size Analyzer (PSA), and water quality tests. Weakening the hemicellulose structure resulted from subjecting the coconut shell to alkali pre-treatment shown in the morphology observation. Furthermore, the FTIR analysis exhibited the presence of O-H stretch in all pre-treated samples representing an indication for occurring the lignin breakdown, while an absence of the C=O functional group was shown in both additive samples after their subjection to alkali pre-treatment. In PSA analysis, the particles of all samples were found finer than the particle size distribution standards, in which the smallest D50 was obtained for the treated coconut shell (TCS), followed by untreated coconut shell (UCS), untreated coconut husk (UCH), and treated coconut husk (TCH). Among all the powdered samples (TCS, UCS, UCH, and TCH) analysed in this study, only TCH values met the ranges provided and recommended by the Urban Stormwater Management Manual for Malaysia (MSMA) and the Australian Department of Sustainability, Environment, Water, Population, and Communities (DSEWPC). Meanwhile, a poor removal rate for total suspended solids (TSS) was attained due to the contribution of TSS by the filter media which caused the TSS rates to surpass the inlet values. Additionally, the presence of a high concentration of total phosphorous (TP) in all the tested samples indicated their capabilities to provide PO43 in the bioretention cell, which is a vital nutrient for the plant’s growth. In contrast, Ammoniacal Nitrogen (AN) with a concentration rate as low as 4 mg/L was observed throughout the test periods which showed a significant reduction compared to the rate of AN at the inlet samples ranged between 5.2 and 11.4 mg/L. The findings indicated that coconut by-products, when subjected to an alkali pre-treatment process, are appropriate for incorporation as additives in bioretention filter media.

Correlation Between Consumption of Nutrient with Milk Production and Milk Quality in Dairy Cow

Abstract The objective of this research is to determine the relationship between consumption of nutrient with milk production and milk quality in dairy cow. Twenty-five dairy cows were used in this research. The dairy cows with 1-3 months of lactation were used in this research. Parameter consumption of nutrient which were used in this research was dry matter, crude protein, total digestible nutrient, calcium and phosphor. Correlation and simple linear regression were used to analyze the data. The results showed strong correlation coefficient and regression equation of consumption of nutrient with milk production (0.618 and Y = 48.675 + 3.95X), milk lactose (0.659 and Y = 6.543 + 0.63X), fat of milk (0.711 and Y = 18.809 + 1.156X) and milk protein (0.659 and Y = 5.313 + 0.579X). Consuming sufficient nutrients to meet the needs of dairy cows can optimize milk production and milk quality.

Reduction of methyltestosterone concentration in feed during masculinization of Nile tilapia (Oreochromis niloticus) in biofloc system

Most larviculture of Nile tilapia is carried out in ponds with a large presence of live food, with temperature variations throughout the day and year, and feed leftoovers due to dissipation in the water. As a consequence, the masculinization process needs to be done with a high concentration of 17α-methyltestosterone (MT) in the diet. Production in closed systems, such as biofloc technology (BFT), allows controlling the water temperature, uses a smaller volume of water and generates less effluent, being an alternative system for the masculinization process. This study evaluated the masculinization rates of Nile tilapia in the BFT, with a controlled temperature (∼28 °C), using concentrations of MT in the feed lower than those recommended for masculinization in ponds and BFT (60 mg ∙ Kg−1 of feed). Tilapia larvae (post yolk sac absorption) were raised in BFT (2 larvae ∙ L−1 or 100 larvae ∙ tank−1) and submitted to different concentrations of MT in the diet (0, 30, 40, 50 and 60 mg ∙ Kg−1 of feed), which was offered five times a day for a period of 28 days. The experimental design was entirely randomized. After the hormonal treatment, the water in the tanks was completely replaced with new water, free of hormonal residues, where the fry remained until they reached a viable size for sexing. It was also tested to maintain the original water used during the hormonal treatment period, during the post-experimental period, where 30 mg ∙ Kg−1 of feed was used. The water quality variables (temperature, pH, dissolved oxygen, settleable solids, total suspended solids, alkalinity, nitrogen compounds, salinity, phosphate and organic carbon) and growth performance variables (final body weight and length, specific growth rate, condition factor and survival) did not differ between MT concentration in the diet (p > .05). The treatment without hormone (control) presented 73.6% of males and the treatments that received hormone showed masculinization rates >99%, significantly higher than control (p < .05), but did not differ from each other (p > .05). The percentage of male individuals in the control treatment was high and this may have occurred because of the temperature of 28 °C throughout the experiment. However, this percentage of males is not considered high enough for production, which requires higher rates close to 100% as possible. The MT was not detected in the water 12 h after the last feeding with feed containing hormone. Thus, it is feasible to use a lower concentration (30 mg of MT ∙ Kg−1 of feed) in BFT. Our study has a direct impact on the global tilapia production scenario, offering a new environmentally sustainable masculinization protocol, using less water and hormones in the process and avoiding the release of hormonal residues.

Effects of low-molecular-weight organic acids on the transformation and phosphate retention of iron (hydr)oxides

The retention and mobilization of phosphate in soils are closely associated with the adsorption of iron (hydr)oxides and root exudation of low-molecular-weight organic acids (LMWOAs). This study investigated the role of LMWOAs in phosphate mobilization under incubation and field conditions. LMWOAs-mediated iron (hydr)oxide transformation and phosphate adsorption experiments revealed that the presence of LMWOAs decreased the phosphate adsorption capacity of iron (hydr)oxides by up to ~74 % due to the competition effect, while LMWOAs-induced iron mineral transformation resulted in an approximately six-fold increase in phosphate retention by decreasing the crystallinity and increasing the surface reactivity. Root simulation in rhizobox experiments demonstrated that LMWOAs can alter the contents of different extractable phosphate species and iron components, leading to 10 % ~ 30 % decreases in available phosphate in the near root region of two tested soils. Field experiments showed that crop covering between mango tree rows promoted the exudation of LMWOAs from mango roots. In addition, crop covering increased the contents of total phosphate and available phosphate by 9.08 % ~ 61.20 % and 34.33 % ~ 147.33 % in the rhizosphere soils of mango trees, respectively. These findings bridge the microscale and field scale to understand the delicate LMWOAs-mediated balance between the retention and mobilization of phosphate on iron (hydr)oxide surface, thereby providing important implications for mitigating the low utilization efficiency of phosphate in iron-rich soils.

Urban stormwater green infrastructure: Evaluating the public health service role of bioretention using microbial source tracking and bacterial community analyses

Bioretention cells (BRCs) control stormwater flow on-site during precipitation, reducing runoff and improving water quality through chemical, physical, and biological processes. While BRCs are effective in these aspects, they provide habitats for wildlife and may face microbial hazards from fecal shedding, posing a potential threat to human health and the nearby environment. However, limited knowledge exists regarding the ability to control microbial hazards (e.g., beyond using typical indicator bacteria) through stormwater biofiltration. Therefore, the purpose of this study is to characterize changes in the bacterial community of urban stormwater undergoing bioretention treatment, with the goal of assessing the public health implications of these green infrastructure solutions. Samples from BRC inflow and outflow in Columbus, Ohio, were collected post-heavy storms from October 2021 to March 2022. Conventional culture-based E. coli monitoring and microbial source tracking (MST) were conducted to identify major fecal contamination extent and its sources (i.e., human, canine, avian, and ruminant). Droplet digital polymerase chain reaction (ddPCR) was utilized to quantify the level of host-associated fecal contamination in addition to three antibiotic resistant genes (ARGs): tetracycline resistance gene (tetQ), sulfonamide resistance gene (sul1), and Klebsiella pneumoniae carbapenemase resistance gene (blaKPC). Subsequently, 16S rRNA gene sequencing was conducted to characterize bacterial community differences between stormwater BRC inflow and outflow. Untreated urban stormwater reflects anthropogenic contamination, suggesting it as a potential source of contamination to waterbodies and urban environments. When comparing inlet and outlet BRC samples, urban stormwater treated via biofiltration did not increase microbial hazards, and changes in bacterial taxa and alpha diversity were negligible. Beta diversity results reveal a significant shift in bacterial community structure, while simultaneously enhancing the water quality (i.e., reduction of metals, total suspended solids, total nitrogen) of urban stormwater. Significant correlations were found between the bacterial community diversity of urban stormwater with fecal contamination (e.g. dog) and ARG (sul1), rainfall intensity, and water quality (hardness, total phosphorous). The study concludes that bioretention technology can sustainably maintain urban microbial water quality without posing additional public health risks, making it a viable green infrastructure solution for heavy rainfall events exacerbated by climate change.

Effect of Phosphate-Deficiency Stress on the Biological Characteristics and Transcriptomics of Panax ginseng

The low availability of phosphorus has become a common problem worldwide. Phosphorus is essential for phenotypic morphology and ginsenoside synthesis. However, the effects of Pi stress on ginseng phenotype and ginsenoside synthesis remain unclear. Phenotypic analyses and transcriptomics revealed the phenotypic construction and regulation of differential genes involved in the physiological metabolism of ginseng under low-Pi stress. Root length and stem length were found to be significantly inhibited by phosphate-deficiency stress in the half-phosphate (HP) and no-phosphate (NP) treatment groups; however, the number of fibrous roots, which are regulated by phytohormones, was found to increase. In ginseng leaves, the indexes of physiological stress, superoxide anion (221.19 nmol/g) and malonaldehyde (MDA) (0.05 μmol/min/g), reached the maximum level. Moreover, chlorophyll fluorescence images and chlorophyll content further confirmed the inhibition of ginseng photosynthesis under low-Pi stress. A total of 579 and 210 differentially expressed genes (DEGs) were shared between NP and total phosphate (TP) and HP and TP, respectively, and only 64 common DEGs were found based on the two comparisons. These DEGs were mainly related to the synthesis of phosphate transporters (PHTs), phytohormones, and ginsenosides. According to KEGG analyses, four DEGs (Pg_s 0368.2, Pg_s3418.1, Pg_s5392.5 and Pg_s3342.1) affected acetyl-CoA production by regulating glycometabolism and tricarboxylic acid cycle (TCA). In addition, related genes, including those encoding 13 PHTs, 15 phytohormones, and 20 ginsenoside synthetases, were screened in ginseng roots under Pi-deficiency stress. These results indicate that changes in the ginseng phenotype and transcriptional regulation of DEGs are involved in the Pi-deficiency stress environment of ginseng, thereby providing new information regarding the development of ginseng for low-Pi tolerance.

Life cycle analysis of the wastewater treatment system in Zabol Industrial Town: Environmental impacts, energy demand, and greenhouse gas emissions.

Use of effective environmental remediation facilities represents a crucial strategy for water reclamation and addressing the challenges of water scarcity. The objective of this study was to assess the wastewater treatment system (WWTS) in Zabol Industrial Town using the life cycle assessment method. Primary data, collected annually for a functional unit of 1 m3 of wastewater treatment, were subjected to analysis using the ReCiPe, Cumulative Energy Demand, and Intergovernmental Panel on Climate Change (IPCC) methods. Human carcinogenic toxicity (50%), freshwater ecotoxicity (13%), and marine ecotoxicity (10%) were the primary environmental impacts due to the WWTS performance. The discharge of heavy metals during sludge generation, coupled with the consumption of natural gas and oil, especially for electricity production, were pivotal factors contributing to the environmental burdens observed. Furthermore, chemical oxygen demand (COD) (56.34%), electricity consumption (>15.47%), and total phosphorous (>4.49%) significantly threatened human health and ecosystem categories, while fossil fuel consumption had the greatest impact on resources. Nonrenewable fossil fuels, namely, natural gas (47.2%) and oil (38.27%), played a predominant role in the energy provision of the system. The IPCC analysis depicted the emissions of CO2 (86.77%) and CH4 (12.16%) stemming from the process of electricity generation. Based on the outcomes of the sensitivity analysis, implementing a 10% increase in COD yielded an increment in all impacts within the range of 1.40% to 6.83%. Given Iran's geographic location and the unique climatic conditions in Zabul, use of solar and wind energy to energize the WWTS can substantially alleviate its environmental burdens. This study presents a comprehensive framework for evaluating the environmental impact, energy consumption, and carbon footprint of a WWTS. Integr Environ Assess Manag 2024;20:1747-1758. © 2024 SETAC.

Sugarcane leaf litter biomass and its effects on increasing sugarcane drought stress tolerance and reducing CO₂ emissions

Burning biomass on sugarcane plantations can lead to pollution (CO₂ emissions) and degradation in soil properties. Adopting non-burning technology can increase the value of biomass by applying it directly to the soil, although this may result in nutrient immobilization, which can hinder optimal plant growth due to high lignin and cellulose content or high CN ratio. On the contrary, composting waste with a decomposer will boost plant nutrient availability and enhance other soil characteristics. This research was aimed to assess the effect of several types of sugarcane organic matter on improving soil characteristics, growth of sugarcane and the CO₂ emissions. The study was conducted using a Completely Randomized Factorial Design with two factors: type of organic matter (control, fresh litter and compost of sugarcane biomass) and level of drought stress (100, 75, 50 and 25%). Incubation occurred over 16 weeks, divided into two phases of 8 weeks each. The results showed that the application of organic materials in the form of compost could reduce water loss caused by environmental heat and sugarcane growing process. Furthermore, compost application improved soil chemical and biological properties by increasing soil pH, total nitrogen (N), total phosphate (P), total potassium (K), and the total microbial population, although differences were not significant compared to the control. Additionally, applying organic matter in the form of compost or litter helped suppress or reduce emissions, with compost treatment proving more effective than litter in reducing CO₂ emissions.

The use of Carica papaya seeds as bio coagulant for laundry wastewater treatment

The study aims to analyze the effectiveness of wastewater treatment by using Carica papaya seeds as bio coagulants to diminish total suspended solids (TSS) and excess phosphate. This method has some advantages because it does not use chemical materials, is simple to apply, and is safe for the environment and human health. The wastewater samples were taken from an active laundry site in Palembang City, South Sumatra Province of Indonesia. The study found that the best dosage of coagulant was 3 g for the wastewater samples. The pH and BOD parameters showed slight changes after treatment, with pH 7.63–7.19 and 33–27 mg/L values, respectively. The TSS removal efficiency ranged from 9.3 to 15.6%, while the COD and phosphate removals were obtained from 11.7 to 39.3% and 56.3 to 68.4%, respectively. The treated TSS, COD, and phosphate concentrations have met the Indonesian domestic wastewater quality standard and environmental protection organization (EPO) guidelines. In addition, the statistical analysis and t-test showed significant differences (p < 0.05) for before and after treatment data of all parameters. The ANOVA test showed significant differences (p < 0.05) for all parameters among the three treatments. Overall, the study indicated that C. papaya seeds are promising materials that are eco-friendly and useful to treat laundry wastewater.