Total Phenolic Content Of Samples Research Articles

Comparison of Extraction Techniques for Determining Bioactive Compounds and Antioxidant Activity of Spirulina platensis

Spirulina platensis (S. platensis) is a high-nutrient blue-green algae that has been used as a food supplement for a long time. It contains carbohydrates, lipids, proteins, vitamins, minerals, and bioactive compounds essential for basic human nutrition. It is known to have anti-cancer, antioxidant, anti-inflammatory, neuroprotective, hepatoprotective, and hypocholesterolemic properties due to the bioactive compounds it contains. In this study, the effects of freeze-thawing, a rapid freezing (-20°C) and thawing (4°C) process, and ultrasonically assisted extraction techniques on the color, antioxidant capacity, total phenolic content, and phenolic composition of Spirulina platensis extracts were investigated. The antioxidant capacity of the extracts obtained was determined by two different methods, DPPH (2,2-diphenyl-1-picryl hydrazyl) and ABTS (2,2-azinobis (3-ethylbenzothiazollin-6-sulfonic acid)). The sugar profile was determined by HPLC-RID and phenolic composition was determined by HPLC-ESI-DAD-MS/MS. The antioxidant activity and total phenolic content of samples prepared by the freeze-thawing were higher than those prepared by ultrasonic-assisted conventional extraction technique. In addition to ferulic acid 4-O-glucuronide and brevifolin carboxylate, an isocoumarin derivative, as the dominant phenolic compound in S. platensis extracts, a total of 10 phenolic compounds including catechin isomer, resveratrol C-hexoside, myricetin, ferulic acid, gallic acid, phloroglucinol, and lutein were detected. Glucose was the predominant sugar in both samples. The total sugar content was higher in the freeze-thawed samples (217.92 mg/100 g DW) than in the ultrasonic-assisted conventional extraction technique (182.91 mg/100 g DW). S. platensis has a significant amount of antioxidants, valuable secondary metabolites, and potential commercial applications and medicinal properties, but releasing these compounds is difficult due to the cell wall. This study was carried out to determine how different extraction techniques alter the release of bioactive compounds.

Optimization of bioactive components of ultrasound treated white grape juice

Grapes are among the most commonly produced fruits worldwide, thanks to their ease of cultivation in terms of climate and soil requirements. Owing to their abundant vitamins, bioactive compounds and minerals incorporating them into one’s diet is advised for promoting human health. The bioactive components of ultrasound-treated white grape juice were optimised by response surface methodology (RSM). In addition, untreated white grape juice (C-WGJ), ultrasound treated white grape juice (U-WGJ) and thermally pasteurized white grape juice (P-WGJ) samples were compared for total phenolics compound (mg GAE/L), total flavonoids (mg CE/L) and DPPH (% Inhibition) parameters. The scientific investigation employed RSM, a widely favored approach. During the study, ultrasound process parameters such as amplitude (40, 50, 60, 70 and 80%) and duration (2, 4, 6, 8 and 10 minutes) were manipulated. The responses to the process application were assessed through analyses of total phenolic content (TPC), total flavonoid content (TFC) and DPPH (1,1-Diphenyl-2-Picryl Hydrazyl). The model achieved commendably high R2 values following optimization through RSM. One-way ANOVA for DPPH value, TFC and TPC of samples, both amplitude and duration exhibited statistically significant effects (p<0.001). The effectiveness of ultrasound treatment in increasing bioactive components in untreated white grape juice is higher than thermal pasteurization treatment. Through the optimization process, the best bioactive values for white grape juice were achieved, measuring 42.3 mg CE/L for TFC, 55.5% inhibition for DPPH and 440.3 mg GAE/L for TPC. Ultrasound applied to white grape juice proved to be more effective in preserving and enhancing bioactive compounds than thermal pasteurization. Consequently, the findings suggest the need for further investigations to assess the impacts of both thermal pasteurization and ultrasound technology on additional quality parameters of white grape juice.

Tropical Cultivated Hericium erinaceus: Antioxidant Capacities of Heat Treatment on Fresh and Oven-Dried Fruiting Bodies

ABSTRACT Consumption of mushrooms in daily diet has become tremendously popular. This study was carried out to investigate the antioxidant capacities (DPPH radical scavenging, reducing power, inhibition of lipid peroxidation, and trolox equivalent antioxidant capacity) and total phenolic content on the heat treatments (boiling, steaming, microwave and pressure cooking) of fresh and oven-dried fruit body of Hericium erinaceus cultivated in tropical region. Samples exposed to 5 minutes to both steaming and pressure-cooking exhibited a significant increase in DPPH and reducing powers activities. The TEAC was also increased significantly in 5 minutes of both microwaving and pressure-cooking samples. Most of the cooked samples showed a high phenolic content. Weak correlation between the antioxidant activities and total phenolic content of samples indicates the antioxidant activity is not due to the amount of phenolic compounds. Thus, steaming and pressure-cooking might be the best cooking method to maintain high antioxidant content of H. erinaceus.

EVALUATE ANTIOXIDANT ACTIVITY, PHENOLIC CONTENT AND COLOUR OF INDONESIAN STINGLESS BEE HONEY AND STING BEE HONEY CULTIVATED IN INDONESIA

ABSTRACT
 Objective: This study's objective was to evaluate the antioxidant activity, phenolic content, and colour of Indonesian stingless and sting bee honey and to assess the correlation between antioxidant, phenolic content, and colour.
 Methods: The Indonesian bee honey sample's antioxidant activity was measured using d 2,2-diphenyl-1- picrylhydrazyl (DPPH). Folin-Ciocalteu (FC) was used to determine the phenolic content. The colour was determined using a colourimeter.
 Results: The values of antioxidant activity (IC50) ranged between 3.58 ± 0.03 µg/ml - 64.27 ± 0.13 µg /ml .H.itama sample from South Sumatra has the highest antioxidant activity, followed by A.dorsata from Bangka Belitung , A. cerana from North Sumatra, T. fuscobalteata from West Nusa Tenggara, and T. biroi South Sulawesi. The total phenolic content of samples ranged from 0.0543 ± 0.003 to 0.1760 ± 0.002 mg GAE/g of honey. The samples A. cerana from North Sumatra, T. biroi from South Sulawesi, A.dorsata from Bangka Belitung, and T. fuscobalteata from West Nusa Tenggara presented the highest quantities of total phenolic contents. The L⁎, a⁎ and b⁎ values ranged (3.08 ± 0.1- 56.19 ± 1.2, 0.845 ± 0.03 - 28.57 ± 0.42 and 1.19 ± 0.22 - 56.51 ± 0.9) respectively. Significant correlation between antioxidant activity, colour, and phenolic content, and of Indonesian bee honey.
 Conclusion: Indonesian bee honey has a different value of antioxidant activity and phenolic content. Differences between Indonesian bee honey samples in terms of type and regions. The dark honey has higher antioxidant activity than light honey.
 Keywords: Antioxidant, Stingless Bee Honey, Sting Bee Honey, Phenolic, Colour.

Evaluation of physicochemical characteristics, antioxidant activity and phenolic profile of Crataegus species in Malatya, Turkey

This study aims to evaluate the pomological and physicochemical characteristics, antioxidant activity and phenolic profile of the three cultivars of hawthorn. A total of nine phenolic compounds were identified in C. orientalis subsp. orientalis fruits by using RP-HPLC-DAD system. These comprised of phenolic acids (i.e., gallic, chlorogenic, caffeic, syringic and p-coumaric acids) and flavonoids (i.e., catechin, epicatechin, rutin and procyanidin-B2). The major phenolic compounds in the hawthorn samples were procyanidin-B2, rutin and chlorogenic acid. The total phenolic contents of samples ranged from 2.86 to 13.81 mg gallic acid equivalent/g dry weight (DW). β-Carotene contents and antioxidant activities (DPPH and ABTS assays) of fruits were found to be high levels. In conclusion, the hawthorn cultivars used in the study differ in composition as they are subspecies of each other. It was concluded that the phenolic compounds of hawthorn are the primary substances responsible for antioxidant activity, and the fruit has shown that it has an important place with these properties. It is thought that this study will lead to the use of hawthorn in the food industry in the future, thus adding value to this fruit and enabling it to be used in new products.

Zeytinyağı üretim aşamalarının zeytinyağının fizikokimyasal özellikleri ve fenolik bileşenleri üzerine etkisi

Aims: In this study, it was aimed to investigate the effect of industrial-scale continuous extraction process stages (crushing, malaxation and decantation) on physicochemical (oil contents, L*, a*, b*, free acidity, peroxide values) and bioactive properties (total phenolic content, antioxidant activity and phenolic compounds) of olive oil. Methods and Results: The samples of olive, olive paste after crushing and malaxation, and olive oil after decantation, which were obtained from olive oil factory, were used for analyses. The highest oil (56.80%) and the lowest total phenolic (160.70 mg L-1) contents were determined after malaxation process at 35°C for 20 min. The free fatty acid value of olive oil increased from 1.68% to 2.46%, but the peroxide value of oil decreased from 12.5 meq O2 kg-1 to 1.5 meq O2 kg-1 after decanter. The L*, a* and b* values of olive oils were determined between 73.82 and 82.04, -12.60 and -3.71, 14.22 and 45.78, respectively. Moreover, phenolic compounds were not significantly affected from industrial oil extraction process (p> 0.05). The minimum oleic acid (68.58%), and the maximum linoleic acid (11.57%) and palmitic acid (14.66%) concentrations were observed in olive oil obtained from malaxed olive paste. Conclusions: The results of oil yield, fatty acid composition and total phenolic content of samples showed significant differences after the malaxation process. Significance and Impact of the Study: It has been determined that the malaxation process is the extraction stage which significantly affects the quality of olive oil.

Sürülebilir keçiboynuzu kreması formülasyonunun karışım deseni ile optimizasyonu

Carob is used as a source of sugar in many product formulations due to its naturally high sugar content. In addition, carob naturally contains a high content of D-pinitol. D-pinitol is an important food ingredient since it regulates glucose metabolism in the body and can be used in the diets of Type 2 diabetic patients. In this study, spreadable cream was produced using carob powder instead of cocoa. Although there are various studies carried out for this purpose in the literature, the optimum formulation was determined for the first time in this study by using optimization methods. The results of the study showed that the formulation has a significant effect on sensorial properties. It was determined that all sensory parameters except consistency increased with increasing sugar syrup ratio. The formulation, which provides the highest overall acceptability (>7), was calculated as 29% carob flour, 32% sugar syrup and 24% fat. It was determined that L *, b * and DPPH radical scavenging activity values could not be statistically modelled. a * and total phenolic content of samples was increased by increment of carob flour in the formulation. As a result, the formulation that maximizes sensorial properties and total phenolic content was calculated as 25% carob flour, 39.3% sugar syrup and 20.7% fat.

INFLUENCE OF WHEY PROTEIN ISOLATE-WAX COMPOSITE EDIBLE COATING ON THE QUALITY OF FRUIT BARS

Composite edible coating of fruit bars using whey protein isolate (WPI) and waxes (beeswax or carnauba wax) was tested in the present study to prevent physicochemical changes during storage in the present study. Dry matter content (96.16-98.43 g/100 g), colour, hardness (54.17-258.16 N), total phenolic content (3097.7-9752.9 mg GAE/kg dm), ascorbic acid content (133.4-203.9 mg/kg dm), antioxidant activity (DPPH: 3681.1-4538.6 mg/kg dm, FRAP: 2531.5-3057.4 mg/kg dm) and peroxide value (1.85-2.06 meq peroxide/kg oil) of samples were determined. Composite edible coating resulted in higher dry matter content and hardness compared to WPI-coated samples. While the total phenolic content of samples coated with WPI+carnauba wax was the lowest, it provided the highest ascorbic acid content and antioxidant activity. A gradual decrease in all analysed parameters except dry matter content and peroxide value was observed throughout the storage period. Overall, edible coating using WPI and carnauba wax composite was suggested for fruit bars.

Impact of refrigerated storage on the bioactive compounds and antioxidant capacity of two Algerian carrot varieties (Daucus carota L.)

Abstract Carrot (Daucus carota L.) is one of the main root vegetables rich in bioactive compounds with appreciable health-promoting properties, largely consumed in Algeria. In the current study, the storage effect (at 4 °C throughout 12 days) on bioactive compound stability and the antioxidant activity of two Algerian orange carrot varieties (Supermuscade and Touchon) were investigated. Total phenolic content of samples was determined by the Folin–Ciocâlteu method. Antioxidant capacity was determined spectrophotometrically, based on the evaluation of Free Radical Scavenging Activity (FRSA) using DPPH radical and Ferric Reducing Power (FRP). The results showed that the Touchon variety is richer in phenolics, flavonoids, and carotenoids and presents higher antioxidant activity in comparison with the Supermuscade variety. At the end of storage, the bioactive compound content and antiradical activity increased significantly (p < 0.05). Also, an extremely significant correlation (p < 0.001) was observed between the antioxidant contents and the antioxidant capacities of aqueous carrot extracts.

Influence of hot and cold break tomato powders on survival of probiotic L. paracasei subsp. paracasei F19, texture profile and antioxidative activity in set-type yoghurts

The yoghurt samples enriched with hot and cold break tomato powders (HBT and CBT) were evaluated by monitoring the changes in some physicochemical properties and bacterial viability during storage. Besides, radical scavenging activity, total phenolic contents, and textural parameters of samples were determined. Tomato powders were added at different levels (0.5, 1, 2%) and L. paracasei F19 was used with starter culture in yoghurt production. Total solids and ash content of yoghurt without powders were lower than the others. HBT supplementation significantly increased DPPH and ABTS + scavenging activities and the highest activities were determined in yoghurt samples containing 2% HBT (197.7 g/kg, 4.84 mol trolox/kg respectively). Total phenolic content of samples ranged from 5.00 to 14.92 g GAE/kg. The addition of tomato powders decreased firmness and viscosity and plain yoghurt was found to be more consistence (12161.80 g s) and cohesive (219.50 g). The pH values of samples decreased whereas titratable acidity values increased throughout 21 days of storage and L. paracasei F19 counts was higher than 106 CFU/g at the end of storage.

Phenolic content and some physical properties of dried broccoli as affected by drying method

Broccoli samples ( Brassica oleracea) with an initial moisture content of 82.87% (wb) were dried using microwave technology (18, 36, and 54 W/g). Convective drying was used as a control group. The dependent variables investigated in the study were phenolic content, color, rehydration capacity, and microstructure of broccoli samples. Moreover, the best fitting thin layer model to the experimental moisture ratio was determined. The phenolic contents were found as 892.4, 740.6, and 759.8 mg gallic acid/100 g dry matter for fresh, convective dried, and microwave (MW) dried at 54 W/g broccoli samples, respectively. The total phenolic content of samples dried at 54 W/g was closest to fresh samples compared to other MW power intensities and convective drying. A similar result was obtained for color values too. L*, a*, and b* of microwave-dried samples at 54 W/g were comparable to fresh broccoli. Another important result obtained from the study was the insignificant effect of drying conditions on rehydration capacity. In addition, it was found that microwave power had a positive effect on drying time; as the microwave powers applied were compared, the shortest drying time was reached at 54 W/g. When the process durations of microwave drying and convective drying were compared, it can be reported that a much lower process time for microwave drying was obtained with respect to convective drying. Drying in microwave oven has reduced the drying time by 49-52%. In the light of the results obtained, it may be declared that it is possible to produce high-quality dried broccoli samples in a very short time by using microwave drying at 54 W/g.

Impact of storage conditions on the bioactive compounds and antioxidant capacity of commercial orange jam

Storage conditions are important factors for fruit jam quality and antioxidant potential. The effects of storage time and temperature on stability of bioactive compounds such as total phenolics and flavonoids and total antioxidant capacity of orange jam were investigated. The jam samples were stored for a period of 30 days at 25 and 35°C. Total phenolic content of samples was determined by the Folin-Ciocalteu method. Antioxidant capacity was determined by spectrophotometric methods and was based on the evaluation of free-radical scavenging activity (FRSA) using DPPH radical and ferric reducing power (FRP). In the current study, the results showed that by the end of storage, the bioactive compound contents and antiradical activity decreased significantly. Also, extremely significant correlation (p < 0.001) was observed between phenolic and flavonoid contents, and antioxidant capacity (FRSA and FRP) of orange jam. Regardless of the phenolic compound loss after storage, orange jams are good sources of substances with antioxidant potential.

Effect of chemical and thermal treatments on quality parameters and antioxidant activity of apple (pulp) grown in high Himalayan regions

AbstractPulp from locally grown Apple variety was given different treatments (chemical and thermal) and its various quality parameters were studied during a storage period of 90 days. All the quality parameters viz. pH, titrable acidity, total soluble solids, reducing sugars and non-reducing sugars, color and antioxidants activity were significantly affected by treatments employed as well as storage period. As a result of chemical and thermal treatments, antioxidant activity and total phenolic content of samples decreased, while color and consumer acceptability significantly improved. Color and antioxidant activity were highly correlated and the type of preservation method used affected the correlation coefficient between color and antioxidant activity. Among different treatments, a combination of pasteurization and chemical preservation was found to be most effective in increasing the shelf life of apple pulp.

UPLC and HPLC of caffeoyl esters in wild and cultivated Arctium lappa L.

Analytical methods including ultra-performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC) with photodiode array (PDA) detector were developed for the analysis of caffeoylquinic acid derivatives in seeds, leaves and roots of Arctium lappa L. Separation was performed on C18 column utilising 5% (v/v) acetic acid in water and acetonitrile at 330nm. Both methodologies were validated in terms of linearity, precision, and recovery. The results showed that the major advantages of UPLC, over HPLC were the fast analysis, narrow peaks, high sensitivity, and reduction of solvent consumption. Subsequently the methods were applied for the identification and quantification of chlorogenic acid (5-CQA) and 1,5-dicaffeoylquinic acid (1,5-DCQA) as main compounds in samples. The total phenolic content of samples ranged from 3.93 to 14.13g of 5-CQA equivalent/100g dry weight (DW). There was a significant variability from 89 to 571mg/100g for 5-CQA and 48 to 486mg/100g for 1,5-DCQA in dry material.

Design of optimal solvent for extraction of bio–active ingredients from six varieties of Medicago sativa

BackgroundExtensive research has been performed worldwide and important evidences were collected to show the immense potential of plants used in various traditional therapeutic systems. The aim of this work is to investigate the different extracting solvents in terms of the influence of their polarity on the extracting ability of bioactive molecules (phenolic compounds) from the M. sativa flowers.ResultsThe total phenolic content of samples was determined using the Folin Ciocalteu (FC) procedure and their antioxidant activity was assayed through in vitro radical decomposing activity using the radical DPPH° assay (IUPAC name for DPPH is (phenyl)–(2,4,6–trinitrophenyl) iminoazanium). The results showed that water was better than methanol and acetic acid for extracting bioactive compounds, in particular for total phenolic compounds from the flowers of alfalfa. The average content of bioactive molecules in methanol extract was 263.5±1.02 mg GAE/100g of dry weight lyophilized extract. The total phenolic content of the tested plant extracts was highly correlated with the radical decomposing activity. However, all extracts were free–radical inhibitors, but the water extract was more potent than the acetic and the methanol ones. The order of inhibitor effectiveness (expressed by IC50) proved to be: water extract (0.924mg/mL) > acetic acid extract (0.154mg/mL) > methanol (0.079mg/mL). The profiles of each extract (fingerprint) were characterized by FT–MIR spectroscopy.ConclusionsThe present study compares the fingerprint of different extracts of the M. sativa flowers, collected from the wild flora of Romania. The total phenolic content of the tested plant extracts was highly correlated with the radical decomposing activity. The dependence of the extract composition on the solvent polarity (acetic acid vs. methanol vs. water) was revealed by UV–VIS spectrometry and Infrared fingerprint.

Screening of Dried Plant Seed Extracts for Adiponectin Production Activity and Tumor Necrosis Factor-Alpha Inhibitory Activity on 3T3-L1 Adipocytes

To search for dried plant seeds with potent anti-diabetes activity, we conducted a large scale screening for inhibitory activity on tumor necrosis factor-alpha and facilitating activity on adiponectin production in vitro. These activities in 3T3-L1 adipocytes were screened from ethanol extracts of 20 kinds of dried plant seed marketed in Japan. komatsuna (Brassica rapa var. perviridis), common bean (Phaseolus vulgaris L.), qing geng cai (Brassica rapa var. chinensis), green soybean (Glycine max), spinach (Spinacia oleracea L.) and sugar snap pea (Pisum sativum L.) markedly enhanced adiponectin production (11.3 ~ 12.7ng/ml) but Japanese radish (Raphanus sativus), edible burdock (Arctium lappa L.), bitter melon (Momordica charantia) and broccoli (Brassica oleracea var. italica) did not (0.9 ~ 2.7ng/ml). All adiponectin-production-enhancing seeds except spinach (2.7pg/ml) and okra (Abelmoschus esculentus) (6.6pg/ml) effectively decreased tumor necrosis factor-alpha levels (0.0pg/ml). We further examined the effects on free radical scavenging activities in the dried seed extracts. Although scavenging activity correlated well with total phenolic content of samples, no correlation was observed with adiponectin production. These results point to the potential of dried seed extracts as a means to modify the activity of tumor necrosis factor-alpha for the adiponectin production.

Extraction of anthocyanins from grape by-products assisted by ultrasonics, high hydrostatic pressure or pulsed electric fields: A comparison

Abstract Extracts from grape by-products contain bioactive substances such as anthocyanins which could be used as natural antioxidants or colourants. The effect of heat treatment at 70 °C combined with the effect of different emerging novel technologies such as ultrasonics (35 KHz), high hydrostatic pressure (600 MPa) (HHP) and pulsed electric fields (3 kV cm− 1) (PEF) showed a great feasibility and selectivity for extraction purposes. After 1 h extraction, the total phenolic content of samples subjected to novel technologies was 50% higher than in the control samples. Therefore, the application of novel technologies increased the antioxidant activity of the extracts being the extractions carried out with PEF four-fold, with HHP three-fold and with ultrasonics two-fold higher than the control extraction. In addition, the extraction of individual anthocyanins was studied showing a selective extraction based on the glucose moieties linked to the anthocyanidins; anthocyanin monoglucosides were better extracted by PEF, whereas the acylated ones were extracted by HHP. Industrial relevance This study examines the feasibility of different emerging technologies such as high hydrostatic pressure, pulsed electric fields and ultrasonics as potential extraction methods for bioactive substances from grape by-products. Grape by-products represent a low-cost source of valuable bioactive compounds such as anthocyanins, with great industrial applications as colourants or nutraceuticals. The higher yields obtained in extractions carried out by high hydrostatic pressure and pulsed electric fields are of major interest from an industrial point of view, since solvent amounts were reduced and extraction times shortened. Thus, the combination of emerging technologies for extraction purposes and low-cost raw materials is an economical alternative to traditional extraction methods according to industry demands and a sustainable development.

Evaluation of the antioxidant power of honey, propolis and royal jelly by amperometric flow injection analysis

In this paper is described the applicability of a flow injection system, operating with an amperometric detector, for measurement in rapid and simple way the antioxidant power of honey, propolis and royal jelly. The proposed method evaluates the reducing power of selected antioxidant compounds and does not require the use of free radicals or oxidants. Twelve honey, 12 propolis and 4 royal jelly samples of different botanical and geographical origin were evaluated by the electrochemical method and the data were compared with those obtained by the DPPH assay. Since a good correlation was found ( R 2 = 0.92) the proposed electrochemical method can be successfully employed for the direct, rapid and simple monitoring of the antioxidant power of honeybee products. Furthermore, the total phenolic content of samples was determined by the Folin–Ciocalteau procedure and the characteristic antioxidant activities showed a good correlation with phenolics ( R 2 = 0.96 for propolis and 0.90 for honey).

Analysis of phenolic compounds in Muscatel wines produced in Portugal

A liquid chromatography method associated with mass spectrometry and diode array, fluorescence and electrochemical detectors was used in order to study phenolic composition of Muscatel sweet wines from Setúbal region in Portugal. Samples were collected during winemaking production at different representative producers of this region. Total phenolic contents of samples were also determined using the Folin–Ciocalteu method. Mass spectrometry results show that atmospheric pressure chemical ionisation (APCI) in negative mode presents higher sensitivity for the majority of the compounds studied. Some phenolic acids, stilbenes as resveratrol and piceid, and flavonols as quercetin and quercetin glycosides were identified in these Muscatel wines. For resveratrol, piceid, gallic acid, protocatechuic acid, catechin and quercetin, fluorescence and electrochemical properties were used as complementary or alternative methods of detection. Differences in phenolic composition and total phenolic contents were found among samples collected.