Total Organic C Content Research Articles

Sugarcane/Soybean Intercropping with Reduced Nitrogen Application Synergistically Increases Plant Carbon Fixation and Soil Organic Carbon Sequestration.

Sugarcane/soybean intercropping and reduced nitrogen (N) application as an important sustainable agricultural pattern can increase crop primary productivity and improve soil ecological functions, thereby affecting soil organic carbon (SOC) input and turnover. To explore the potential mechanism of sugarcane/soybean intercropping affecting SOC sequestration, a two-factor long-term field experiment was carried out, which included planting pattern (sugarcane monocropping (MS), sugarcane/soybean 1:1 intercropping (SB1), and sugarcane/soybean 1:2 intercropping (SB2)) and nitrogen addition levels (reduced N application (N1: 300 kg·hm-2) and conventional N application (N2: 525 kg·hm-2)). The results showed that the shoot and root C fixation in the sugarcane/soybean intercropping system were significantly higher than those in the sugarcane monocropping system during the whole growth period of sugarcane, and the N application level had no significant effect on the C fixation of plants in the intercropping system. Sugarcane/soybean intercropping also increased the contents of total organic C (TOC), labile organic C fraction [microbial biomass C (MBC) and dissolved organic C (DOC)] in the soil during the growth period of sugarcane, and this effect was more obvious at the N1 level. We further analyzed the relationship between plant C sequestration and SOC fraction content using regression equations and found that both plant shoot and root C sequestration were significantly correlated with TOC, MBC, and DOC content. This suggests that sugarcane/soybean intercropping increases the amount of C input to the soil by improving crop shoot and root C sequestration, which then promotes the content of each SOC fraction. The results of this study indicate that sugarcane/soybean intercropping and reduced N application patterns can synergistically improve plant and soil C fixation, which is of great significance for improving crop yields, increasing soil fertility, and reducing greenhouse gas emissions from agricultural fields.

Changes in the Glucose Concentration Affect the Formation of Humic-like Substances in Polyphenol-Maillard Reactions Involving Gibbsite.

The polyphenol-Maillard reaction is considered one of the important pathways in the formation of humic-like substances (HLSs). Glucose serves as a microbial energy source that drives the humification process. However, the effects of changes in glucose, particularly its concentration, on abiotic pathways remain unclear. Given that the polyphenol-Maillard reaction requires high precursor concentrations and elevated temperatures (which are not present in soil), gibbsite was used as a catalyst to overcome energetic barriers. Catechol and glycine were introduced in fixed concentrations into a phosphate-buffered solution containing gibbsite using the liquid shake-flask incubation method, while the concentration of glucose was controlled in a sterile incubation system. The supernatant fluid and HLS components were dynamically extracted over a period of 360 h for analysis, thus revealing the influence of different glucose concentrations on abiotic humification pathways. The results showed the following: (1) The addition of glucose led to a higher degree of aromatic condensation in the supernatant fluid. In contrast, the supernatant fluid without glucose (Glu0) and the control group without any Maillard precursor (CK control group) exhibited lower degrees of aromatic condensation. Although the total organic C (TOC) content in the supernatant fluid decreased in all treatments during the incubation period, the addition of Maillard precursors effectively mitigated the decreasing trend of TOC content. (2) While the C content of humic-like acid (CHLA) and the CHLA/CFLA ratio (the ratio of humic-like acid to fulvic-like acid) showed varying increases after incubation, the addition of Maillard precursors resulted in a more noticeable increase in CHLA content and the CHLA/CFLA ratio compared to the CK control group. This indicated that more FLA was converted into HLA, which exhibited a higher degree of condensation and humification, thus improving the quality of HLS. The addition of glycine and catechol without glucose or with a glucose concentration of 0.06 mol/L was particularly beneficial in enhancing the degree of HLA humification. Furthermore, the presence of glycine and catechol, as well as higher concentrations of glucose, promoted the production of N-containing compounds in HLA. (3) The presence of Maillard precursors enhanced the stretching vibration of the hydroxyl group (-OH) of HLA. After the polyphenol-Maillard reaction of glycine and catechol with glucose concentrations of 0, 0.03, 0.06, 0.12, or 0.24 mol/L, the aromatic C structure in HLA products increased, while the carboxyl group decreased. The presence of Maillard precursors facilitated the accumulation of polysaccharides in HLA with higher glucose concentrations, ultimately promoting the formation of Al-O bonds. However, the quantities of phenolic groups and phenols in HLA decreased to varying extents.

Total Carbon and Nitrogen and Granulometric Fractions of Soil Organic Matter Under No-Till System and Conventional Tillage with Onion Cultivation

Objective: The objective of this work was to evaluate the total carbon (C) and nitrogen (N) contents and the granulometric fractions of soil organic matter (SOM) under different management systems with single and intercropped winter cover crops in the cultivation of onion in Ituporanga, SC. Theoretical framework: Onion cultivation typically occurs in a conventional tillage system (CTC), which favors the loss of C and N from the soil due to soil mobilization. The vegetable no-tillage system (VNTS) uses sustainable practices with continuous soil cover and restricted soil tillage, promoting improvement of edaphic attributes. Methodology: The experiment was implemented in 2009 and the collection of soil samples took place in 2019 in the layers of 0-5, 5-10 and 10-30 cm in the treatments under VNTS, being black oat (AV); rye (CE); oilseed radish (NB); rye + radish (CE+NB); black oat + oilseed radish (AV+NB) and spontaneous vegetation (VE). In addition to these treatments, two adjacent areas were used, one under CTS and the other in a secondary forest area (MATA). The content of total organic C (TOC), total N (NT), light organic matter in water (LOM), particulate organic carbon (POC), particulate nitrogen (PN), C associated with minerals (COam) and N associated with to minerals (NAM). Results and conclusions: VNTS increased TOC and NT contents with the use of grass and cruciferous intercropping to the detriment of single crops and CTS. The LOM content was higher in VNTS with the use of single grasses and stood out in relation to VNTS with consortium. There were no differences for PN between MATA, CE and CE+NB, demonstrating that VNTS is efficient in raising these levels in substitution of CTS. The CE+NB consortium increased the levels of COam and NAM in the surface layer of the soil after replacing CTS with VNTS. Research implications: In VNTS, the use of intercropping between cover crops is an efficient management in adding C and N to the soil, as the simultaneous cultivation of different species of cover crops produces straw with an intermediate C/N ratio, the roots exploit different soil extracts and nutrient cycling is more efficient. Originality/Value: The results of this study are from a long-term experiment (10 years), which demonstrates the effectiveness of VNTS in recovering and increasing C and N levels in soil previously cultivated for a long period in the CTS, in addition to to match the forest area.

Exploring the Role of Stumps in Soil Ecology: A Study of Microsite Organic Carbon and Enzyme Activities in a Larix olgensis Henry Plantation

Stumps are a significant component of coarse woody debris in plantations, but their effect on microsite soil organic carbon (C) and enzyme activities remains understudied. Soil (Alfisol) samples were collected at varying distances from larch (Larix olgensis Henry) stumps and at different soil depths (0–20 cm and 20–40 cm) to analyze soil total organic C (TOC), particulate organic C (POC), easily oxidizable C (EOC), microbial biomass C (MBC), and enzyme activities. Results indicated that stumps significantly affected TOC and POC contents, with the greatest horizontal range of impact reaching up to 15 cm in both the topsoil and subsoil layers. Stumps also significantly affected MBC content, with the greatest horizontal range of impact reaching up to 55 cm in the subsoil layer. EOC content was the most affected, with the stumps’ impact extending to 55 cm in both soil layers. Additionally, the study showed that stumps had a significant impact on the activities of β-glucosidase and β-cellobiohydrolase, with the greatest horizontal range of impact reaching up to 15 cm for glucosidase and 35 cm for cellobiohydrolase in the topsoil layer. Stumps also significantly affected the activities of phenol oxidase and peroxidase, with the maximum horizontal range of stump impact extending up to 35 cm for phenol oxidase and 55 cm for peroxidase in the topsoil layer. This study enhances our understanding of the role of stumps in plantation ecosystems and offers valuable insights for future management strategies to maintain soil fertility and improve site productivity.

Effect of Exogenous Glucose at Different Concentrations on the Formation of Dark-Brown Humic-like Substances in the Maillard Reaction Pathway Based on the Abiotic Condensation of Precursors Involving δ-MnO2

The Maillard reaction is a type of nonenzymatic browning process and is an important pathway for the formation of humic-like substances (HLSs). Glucose is one of the three crucial precursors for the Maillard reaction, and a change in glucose concentration can inevitably affect the humification pathway, thereby regulating the composition and quality of HLSs. To verify the scientific hypothesis, the method of liquid shake-flask culture was adopted. Both catechol and glycine with fixed concentrations were added to a phosphate buffer including δ-MnO2, and only the concentration of glucose was adjusted in the sterile culture system. The obtained supernatant fluid and dark-brown residue were collected dynamically through the centrifugation method. The E4/E6 ratio and total organic C (TOC) of the supernatant fluid, the humus composition, and FTIR spectra for the dark-brown residue, and the elemental composition of humic-like acid (HLA) extracted from the dark-brown residue were analyzed to reveal the effect of varying glucose concentrations on the abiotic humification pathways for the Maillard reaction and the characteristics of relevant products under abiotic processes. The results reveal that (1) the exogenous addition of glucose at different concentrations simplifies the molecular structure in the supernatant fluid, and the TOC content is decreased to varying degrees, among which the addition of 0.24 mol/L glucose leads to the formation of simpler organic molecules in the supernatant compared to that for the other treatments, and the addition of 0.03 mol/L glucose shows the largest decrease in TOC content; (2) Under the coexistence of glycine and catechol, CHLA treated with the addition of glucose at different concentrations shows an upward trend in the course of the culture, which is significantly higher than that obtained for the CK control. The addition of 0.12 mol/L glucose results in the largest increase in CHLA. During the culture period, the structure of HLA molecules from each treatment first become complex and then gradually become simpler. Finally, the molecular structure of HLA treated with different concentrations of glucose becomes more complex, but the structure of HLA molecules from the CK control tends to be simplified. The addition of glucose can improve the condensation degree of HLA molecules, among which the addition of 0.12 mol/L glucose shows the most significant effect. With increasing exogenous glucose concentration, the number of N-containing compounds in the HLA molecules further decreases, while the number of O-containing functional groups increases. (3) The greater the concentration of glucose added, the higher the proportion of aromatic C structures in the dark-brown residue. During this process, the Mn-O bond lattice vibration of the δ-MnO2 layered structure is greatly enhanced. The organic molecules in the dark-brown residue and δ-MnO2 are bound to each other through intermolecular hydrogen bonding. The CHLA/CFLA ratio for each treatment increases to varying degrees after the culture period, indicating that the addition of glucose is more conducive to the improvement of humus quality than the CK control, among which the addition of 0.12 mol/L glucose shows the best effect.

Chemical Characteristics of Dark-Brown Humic-like Substances Formed from the Abiotic Condensation of Maillard Precursors with Different Glycine Concentrations

The Maillard reaction is a type of nonenzymic browning process, and it is also an abiotic humification process of sugars, amino acids and phenols catalyzed by δ-MnO2. It is considered to be one of the possible pathways for the formation of humic-like substances (HLS). The change in the ratio of the Maillard precursors inevitably affects the chemical characteristics of HLS, among which the effect of amino acids concentration on the humification pathway and HLS formation has not yet been reported. In view of this, the glucose, glycine and catechol were chosen as tested objects for the present study, and the method of liquid shake-flask culture was adopted. Both catechol and glucose with fixed concentrations were added into a phosphate buffer solution (pH 8.0) containing δ-MnO2, and only the glycine concentration was adjusted in the sterile culture system. The supernatant solution and dark-brown residue were collected dynamically within 360 h through the centrifugation method. The E4/E6 ratio and total organic C (TOC) of the supernatant solution, the C content of the humic-like acid (CHLA), CHLA/CFLA (C content of fulvic-like acid) ratio and FTIR spectra of the dark-brown residue and the E4/E6 ratio and atomic ratio of humic-like acid (HLA) extracted from the dark-brown residue were systematically analyzed to reveal the effect of different glycine concentrations on the abiotic humification pathways and the characteristics of related products from the Maillard reaction under abiotic processes. The results showed that (1) Under the influence of the addition of different glycine concentrations, the structure of organic molecules in the supernatant after culture tended to be simplified, and the addition of three lower concentrations (0, 0.03 and 0.06 mol/L) of glycine made the E4/E6 ratios increase by 100.4%, 57.7% and 33.0%, respectively, and obtained a simpler structure of organic molecules in the supernatant than that of 0.12 and 0.24 mol/L glycine, which made the E4/E6 ratios increase by 5.6% and 18.0%, merely. After culture, the TOC content in the supernatant solution of each treatment decreased to varying degrees, and the addition of Maillard precursors effectively inhibited the loss of TOC in the supernatant solution, especially the addition of glycine at a concentration of 0.06 mol/L, which only reduced the TOC content by 0.1%. (2) The greater the concentration of glycine added, the higher proportion of aromatic C structure existed in the dark-brown residue. O-containing functional groups from the dark-brown residue and δ-MnO2 were bound to each other through hydrogen bonding, and (3) During the culture process, the CHLA treated with the addition of Maillard precursors was significantly higher than that of the CK control. Compared with the result at 0 h, the addition of higher concentrations of glycine (0.12 and 0.24 mol/L) were more conducive to the formation of HLA, making the CHLA increase by 666.2% and 422.7%, which were much more than these results for 256.6%, 282.2% and 360.0% from three concentrations of glycine (0, 0.03 and 0.06 mol/L) at the end of culture. After culture, the structure of HLA molecules treated by the addition of Maillard precursors became more complex, and the overall performance showed that the higher the concentration of added glycine, the more complex the HLA molecules became. Under the coexistence of glucose and catechol, the addition of glycine could promote abiotic condensation and improve the condensation degree of HLA molecules, among which the addition of 0.12 mol/L glycine had the most significant effect. Compared with the CK control, the addition of Maillard precursors could achieve a higher increase in the CHLA/CFLA ratio, which was more beneficial to the improvement of humus quality.

Origin of carbon in agricultural soil profiles deduced from depth gradients of C:N ratios, carbon fractions, \u03b413C and \u03b415N values

AimsAgricultural soils in Germany store 2.54 Pg of organic carbon (C). However, information about how and when this C entered the soils is limited. This study illustrates how depth profiles of organic matter can shed light on different entry paths of organic C.MethodsMachine learning was used to explain total organic C (TOC), C:N, particulate organic C (POC), δ13C and δ15N values down to 100 cm depth based on pedology, geology, climate and management-related variables from the German Agricultural Soil Inventory. We estimated TOC turnover rates based on the relationship between the proportion of maize (only C4 plant) in crop rotations and soil δ13C values.ResultsIn the upper 30 cm of cropland, fresh photosynthates added on average 0.2 to 0.8 Mg C ha− 1 year− 1. Organic fertiliser was another source of topsoil C, especially in grassland. Sandy sites in north-west Germany contained historic C from past heathland and peatland. One third of German agricultural land was found to be on colluvial and alluvial deposits, in which allochthonous C from upstream and upslope areas evidently increased the TOC content of subsoils. In and below hardpans, TOC content and C:N and POC:TOC ratios were low, indicating restricted root-derived C input.ConclusionsOur data indicate that ongoing management in German agricultural soils mainly affects topsoil C, while C storage in subsoils reveals significant legacies from allochthonous, buried or translocated C inputs. Specific attention should be focused on the sustainable loosening of hardpans that could result in a slow, but significant increase in subsoil C stocks.

Total organic carbon concentrations in ecosystem solutions of a remote tropical montane forest respond to global environmental change

The response of organic carbon (C) concentrations in ecosystem solutions to environmental change affects the release of dissolved organic matter (DOM) from forests to surface and groundwaters. We determined the total organic C (TOC) concentrations (filtered <1-7µm) and the ratios of TOC/dissolved organic nitrogen (DON) concentrations, electrical conductivity (EC), and pH in all major ecosystem solutions of a tropical montane forest from 1998 to 2013. The forest was located on the rim of the Amazon basin in Ecuador and experienced increasing numbers of days with >25°C, decreasing soil moisture, and rising nitrogen (N) deposition from the atmosphere during the study period. In rainfall, throughfall, mineral soil solutions (at the 0.15- and 0.30-m depths), and streamflow, TOC concentrations and fluxes decreased significantly from 1998 to 2013, while they increased in stemflow. TOC/DON ratios decreased significantly in rainfall, throughfall, soil solution at the 0.15-m depth, and streamflow. Based on Δ14 C values, the TOC in rainfall and mineral soil solutions was 1year old and that of litter leachate was 10years old. The pH in litter leachate decreased with time, that in mineral soil solutions increased, while those in the other ecosystem solutions did not change. Thus, reduced TOC solubility because of lower pH values cannot explain the negative trends in TOC concentrations in most ecosystem solutions. The increasing TOC concentrations and EC in stemflow pointed at an increased leaching of TOC and other ions from the bark. Our results suggest an accelerated degradation of DOM, particularly of young DOM, associated with the production of N-rich compounds simultaneously to changing climatic conditions and increasing N availability. Thus, environmental change increased the CO2 release to the atmosphere but reduced DOM export to surface and groundwater.

Use of Brassica juncea and Dactylis glomerata for the phytostabilization of mine soils amended with compost or biochar

Phytostabilization of mine soils contaminated by potentially toxic elements (PTEs) requires plants tolerant to PTE toxicity and to the poor soil physico-chemical characteristics of these areas. A pot experiment was carried out to assess the phytostabilization potential of Brassica juncea and Dactylis glomerata in mine soils amended with compost and biochar. Furthermore, the Environmental Risk of the soils and the effects of the phytostabilization process on the microbiological population size and activity in the soils were also determined. According to the Ecological Risk Index (ERI) the soils studied presented “very high risk” and As, Cd and Pb were the target elements for phytostabilization. Both amendments improved soil conditions (e.g., increasing total-N and total organic-C concentrations) and contributed to PTE (Cd, Pb and Zn) immobilization in the soil. Compost showed a more marked effect on soil microbial biomass and nutrients release in soil, which led to higher B. juncea and D. glomerata biomass in compost treated soils. Biochar treatment showed a positive effect only on D. glomerata growth, despite it provoked strong PTE immobilization in both soils. The addition of both amendments resulted in an overall reduction of PTE concentration in the plants compared to the control treatment. In addition, both plant species showed higher accumulation of PTE in the roots than in the shoots (transfer factor<1) independently of the treatment received. Therefore, they can be considered as good candidates for the phytostabilization of PTE contaminated mine soils in combination with organic amendments like biochar and compost.

Aggregation index, carbon, nitrogen, and natural abundance of 13C and 15N in soil aggregates and bulk soil cultivated with onion under crop successions and rotations

Use of soil cover crops of different families in crop rotation or succession under no-tillage system (NTS) for onion production results in higher soil quality compared to land use systems with less plant diversity. The objective was to evaluate the effect of using different combinations of plant species from different botanical families in rotation and succession of soil cover crops in NTS for onion production on formation of macroaggregates, mesoaggregates, and microaggregates, and on total organic C (TOC) and N (TN) contents, including isotopic forms of C and N, in soil aggregates and bulk soil. The treatments (T) evaluated were maize/onion (NTS-T1); cover plants (winter)/onion (NTS-T2); maize/winter grasses/onion (NTS-T3); velvet bean/onion (NTS-T4); millet/cover plants (winter)/onion (NTS-T5); velvet bean/rye/onion (NTS-T6); maize/onion in conventional tillage system (CTS-T7); and intercrop cover plants (summer)/onion (NTS-T8). We evaluated macroaggregates (8.0–0.25 mm), microaggregates (&amp;lt;0.25 mm), and bulk soil (&amp;lt;2.0 mm) at depths of 0–5, 5–10, and 10–20 cm, in a nine-year field experiment. The greater plant diversity in T2–T6 and T8 resulted in higher geometric mean diameter (GMD) of aggregates compared to T1 and T7. The T8 was more efficient in increasing GMD in the 10–20 cm soil depth than the other treatments. The T1 was more efficient in improving the evaluated soil physical and chemical attributes than T7. The use of NTS with plants of the Poaceae and Fabaceae families in single or intercrop systems for onion production resulted in higher TOC and TN contents in the 0–5 and 5–10 cm soil depths compared to CTS. Isotope 15N measurements showed that C and N were more protected in microaggregates in all evaluated treatments and depths compared to macroaggregates and bulk soil. Macroaggregates had more TOC and TN than microaggregates.

Quantifying total and labile pools of soil organic carbon in cultivated and uncultivated soils in eastern India

Loss of labile carbon (C) fractions yields information about the impact of land-use changes on sources of C inputs, pathways of C losses and mechanisms of soil C sequestration. This study dealt with the total organic C (TOC) and labile C pools in 40 surface soil samples (0–15 cm) collected from four land-use practices: uncultivated sites and rice–wheat, maize–wheat and sugarcane agro-ecosystems. Uncultivated soils had a higher total C pool than croplands. The soil inorganic C concentrations were in the range of 0.7–1.4 g kg–1 under different land-use practices. Strong correlations were found between TOC and all organic C pools, except water-extractable organic C and mineralisable C. The sensitivity index indicated that soil organic C pools were susceptible to changes in land-use practices. Discriminant function analysis showed that the nine soil variables could distinguish the maize–wheat and rice–wheat systems from uncultivated and sugarcane systems. Finally, we recommend crop rotation practices whereby planting sugarcane replenishes TOC content in soils.

Short-term effects on soil of biogas digestate, biochar and their combinations

The use of the residual material from waste aerobic digestion and biochar as amendments is currently discussed in the literature concerning the positive and negative effects on soil quality. We assessed the suitability of digestate (D) from biogas production and green biochar (B) to improve soil biological activity and antioxidant capacity and investigated whether there is an interaction between digestate and biochar applied to soil in combination. In a short-term (100-days) laboratory incubation, we monitored soil chemical and biological parameters. We compared soil amendments with 1% D (D1), 5% D (D5), 1% B (B), digestate–biochar combinations (D1+B and D5+B), and soil with no amendment. In D5, CO2 production, antioxidant capacity (TEAC), and dehydrogenase activity (DH-ase) and the contents of microbial biomass C, DOC and alkali-soluble phenols increased to the highest level. The biochar increased the total organic C (TOC) and TEAC of soil but decreased DOC, CO2 production, microbial biomass C, and DH-ase. The addition of biochar to digestate reduced soluble compounds (DOC and phenols), thus limiting the amount and activity of the soil microbial biomass (CO2 production and DH-ase). After 100 days of incubation D5+B showed the highest TOC content (82.8% of the initial amount). Both applied alone and in combination with digestate, the biochar appears to enrich the soil C sink by reducing CO2 emissions into the atmosphere.

Land-use impacts on profile distribution of labile and recalcitrant carbon in the Ili River Valley, northwest China

There is a growing evidence that the decomposition of recalcitrant carbon (C) can be stimulated by environmental changes, such as fresh C supply and increased temperature. However, the effect of land-use on profile distribution of recalcitrant C content is still poorly understood. In this study, soil samples were collected to a depth of 100cm from pastures and four major croplands including maize field, wheat field, paddy and apple orchard in the Ili River Valley, northwest China, to investigate the effects of land-use on profile distribution of labile organic C (LOC), semi-labile organic C (SLOC), recalcitrant organic C (ROC) and their relative proportions in total organic C (TOC), and evaluate whether such effects can be different between topsoil (0–20cm) and subsoil (20–100cm). The results showed that soil ROC accounting for 49.4–66.3% of TOC for different land-uses, implying that ROC is the major form of soil organic C (SOC). Soil TOC contents of croplands were 20.4–85.2% lower than those of pastures along the soil profile, indicating that SOC pool may be decreased by agricultural land-uses. The lower contents of LOC, SLOC and ROC in croplands than in pastures suggested that the decreases in TOC content in croplands are not only due to the decreases in labile C pool but also the reductions in recalcitrant C pool. The differences in SOC fractions among land-uses were similar in topsoil and subsoil, while the proportions of each SOC fraction in TOC did not differ significantly between the two soil layers in most cases, indicating that each SOC fraction in subsoil can be also influenced by land-use types. Therefore, it is suggested that the ROC in subsoil, which plays a crucial role in C sequestration, should be taken into account when estimating the effect of land-use on SOC kinetic.

Soil carbon sequestration and nutrient status of tropical rice based cropping systems: Rice-Rice, Rice-Soya, Rice-Onion and Rice-Tobacco in Sri Lanka

Carbon sequestration increases soil fertility and reduces global warming by storing atmospheric carbon in soils. This study aimed to quantify and compare soil organic C fractions and C stocks in 4 different rice based cropping systems and investigate their variation as affected by crop rotation with upland crops. Soil nutrient availability and their relationship with chemical C fractions were also examined. Total organic C (TOC), microbial biomass C (MBC), water soluble C (WSC), KMnO4 oxidizable C, pH and available macronutrients were analyzed at 0–15 and 15–30cm depths in Rice-Rice11RR; Rice-Rice, RS; Rice-Soya, RT; Rice-Tobacco, RO; Rice–Onion. (RR), Rice-Soya (RS), Rice-Tobacco (RT) and Rice–Onion (RO) rotations on Alfisols of Sri Lanka. The data were analyzed by analysis of variance (ANOVA) on a completely randomized design (CRD) under 4 treatments with 6 replicates for each treatment. Results showed that carbon fractions and nutrient availability among different cropping systems varied significantly from one another. Under all cropping systems a higher content of all C fractions was observed in the 0–15cm layer, except for WSC which was higher in the 15–30cm layer because it moves to the deeper layers. The top soil layer also had a higher amount of MBC than the deeper layer, because the amount of microbial biomass and rate of microbial activity decline rapidly below the surface layer. Highest dry matter return to soil (147g/m2) in the RR system as paddy stubble accounted for highest amount of TOC in soil. RS system also had a higher TOC content due to the organic residues collected in soil as roots and leaf litter (67.7g/m2) as all dry leaves have fallen on to the soil at the time of harvesting of soyabean. However in RO system hardly any residues are added because the entire crop is removed at harvest and a significantly lower organic C content was recorded. Also the soil in RO and RT were tilled more than that used for soyabean during temporary bed preparation resulting in a relatively faster decomposition leaving significantly lower levels of C compared to RS. Change of cropping systems from RR to other annual crops such as RT and RO reduced the soil C sequestration to a significant level after 10years of cultivation. However crop rotation change from RR to RS has maintained similar levels of C (65.18t/ha) in RS and (63.48t/ha) in RR. This indicated that C sequestration capacity is species specific and differences are mainly due to remaining crop residues and specific soil tillage practices used for upland crops. The study also showed that soil nutrient availability among the cropping systems varied significantly. Correlation analysis between chemical C fractions and major nutrient cations showed that there are significant correlations exist among them. The study confirmed that tropical rice based cropping systems have a great potential in storing and maintaining C in soils and thereby to facilitate nutrient availability.

Legacy Effect of Fertilization and Tillage Systems on Nitrogen Mineralization and Microbial Communities

Core Ideas Soil C and N dynamics were still affected by past fertilization treatments (8 yr). Soil C and N dynamics were still affected by past tillage systems (3 yr). Ammonia‐oxidizing bacteria were affected by ammonium concentration and pH. Ammonia‐oxidizing bacteria and archaea were associated with N mineralization. Poultry litter (PL) is widely used as an alternative to inorganic fertilizers (IF) in conventional (CT) and no‐tillage systems (NT). The objective of this work was to evaluate the legacy effect of a 16‐yr factorial study that combined fertilization (PL or IF) and tillage (CT or NT) treatments on soil C and N pools and microbial communities. Soil samples were collected from the 0 to 5 and 5 to 20 cm depths after 8 and 3 yr of suspending fertilization and tillage, respectively. Samples were analyzed for total organic C (TOC), total organic N (TON), N mineralization rate (k0), and abundance of ammonia‐oxidizing bacteria (AOB) and archaea (AOA). The TOC and TON concentrations (range: 5.8–20.8 and 0.5–1.8 g kg−1, respectively) were affected by tillage system and fertilizer source. Legacies of fertilization and tillage affected k0 (range: 0.20–0.87 mg kg−1 d−1) which was correlated with TOC and TON (r2 ∼ 0.9). The abundance of AOA (5.3–7.6 log10 copies g−1) and AOB (7.1–8.9 copies log10 g−1) was greater in CT than NT in the 0 to 5 cm layer, whereas the opposite was observed in the 5 to 20 cm layer. Both AOA and AOB were associated with k0 (r2 = 0.5 and 0.8, respectively), suggesting the dependence of ammonia oxidizers on mineralization of the organic N pool. Our results indicate that 8 and 3 yr after fertilization and tillage treatments were suspended, soil C and N and microbial dynamics were still being influenced by previous management practices.

Dynamics of soil carbon, nitrogen, and phosphorus in calcareous soils after land-use abandonment – A chronosequence study

The objective of this study was to investigate changes in soil total organic C (TOC), total nitrogen (TN), phosphorus (P) fractions, and microbial community structure during secondary succession after abandonment of vineyards on calcareous soils. Two chronosequences covering 200 years and differing in aspect and slope were established in Hungary, and the upper 10 cm of the mineral soils were studied. We found strong increases in TOC concentrations after land-use abandonment, especially at the south-exposed sites. The TOC/TN ratio increased by a factor of 1.3 in the south-west exposed chronosequence and by a factor of 1.6 in south exposed chronosequence. The concentration of labile P (NaHCO3-extractable P) diminished during the first 50 years after land-use abandonment, leading to low P availability at the later stages of the succession. The total organic P (TOP) concentration increased during the first 40 years after abandonment. At the later stages of succession, TOP concentrations decreased again, while the ratio of TOC/TOP increased continuously over 200 years. The ratio of arbuscular-mycorrhizal-fungi-to-bacteria (AMF/bacteria) increased strongly during the first decade after abandonment of the vineyards. Our study indicates that impacts of former cultivation on secondary ecosystems persisted for more than a century, and that especially P concentrations showed long lasting legacy effects.

Effects of amendment with oil mill waste and its derived-compost on soil chemical and microbiological characteristics and olive (Olea europaea L.) productivity

A new type of solid oil mill waste (SOMW), produced by latest-generation decanter, and its derived compost were spread on land for three consecutive years in an olive grove to evaluate the soil chemical characteristics, bacterial abundance and community structure, plant growth and production and oil quality. After the third year of the experiment in both treatments, there was no increase in total organic C (TOC) in the upper layer, while a reduction of TOC concentration in the deeper layer was detected. Moreover, in the upper soil layer, the available P increased in both treated soils compared to control while exchangeable K increased only in SOMW treated soil. In both the treated soils, viable counts of different bacteria showed little or no differences compared with the control. Further, PCR-DGGE analysis of the soil bacterial community indicated the presence of a very high biodiversity that was not affected by the long-term treatment with either SOMW and composted-SOMW. The treated soils with both matrices showed an increase in the vegetative activity and olive yield such as a positive effect on oil quality by increasing phenol content. The overall results indicate that both SOMW and its derived-compost can be positively used as soil amendment, thus, contributing to the reduction in the use of chemical fertilizers.

The relationship between soil organic carbon and soil surface characteristics in the semi-arid rangelands of southern Australia

The potential carbon sequestration in rangelands is largely due to the extensive areas they occupy, even though levels of soil organic carbon (SOC) are low. There is considerable uncertainty in achieving this potential due to the inherent patchy spatial and temporal distribution of rangeland vegetation and resources. At a paddock scale, determining appropriate sampling scales is a critical first step in the accurate estimation of size and spatial distribution of stocks of SOC. This issue was addressed by examining the spatial distribution of SOC and determining the association of SOC with other site characteristics such as ground cover and vegetation. This was done in a pilot study conducted in a 136-ha paddock located on the Cobar Pediplain Bioregion in western New South Wales, Australia. Each of 104 sites was sampled using a 0.25-m2 quadrat to assess biomass and ground cover category (percentage of perennial plants, bare ground, cryptogams, annual plants and litter) of a soil core taken from the centre of each quadrat, and proximity to trees and shrubs. The soil core was used to determine total organic C (TOC), total N (TN) and the C : N ratio at four depths (0–5; 5–10; 10–20; 20–30 cm). From the quadrat and ground cover categories of the soil cores, six microsite categories were identified using cluster analysis: cryptogams; litter (≥25% litter); bare (≥60% bare ground); annual (≥40% annual plants); litter-P (≥15% litter and ≥10% perennial plants) and perennial (≥30% perennial plants). Microsite, depth in soil profile and the presence of trees and shrubs all had a significant (P &lt; 0.001) effect on TOC concentration. The predicted means (s.e. of mean) of TOC at the soil surface (0–5 cm) were perennial 1.26 (0.04) %; litter-P 1.20 (0.05) %; annual 1.18 (0.06) %; litter 1.12 (0.05) %; bare 1.03 (0.05) % and cryptogams 0.88 (0.06) %. Higher concentrations of TOC were associated with the presence of trees and were almost 30% higher in close proximity (&lt;1 m) to a tree. There was a consistent finding that higher concentrations of TOC, TN and the high values of C : N ratio were each associated with higher ground cover of perennial plants. The autocorrelation range for soil C stocks was ~30 m and for categories of ground cover which varied from 10 m to over 200 m. The spatial predictions for ground cover of perennial plants closely mirrored those for C stocks, which were 22.9 Mg C ha–1 in the top 30 cm of soil in this environment. As both tree proximity and ground cover had a significant effect on TOC, quantifying the levels of soil organic C at a paddock scale will require an understanding of the spatial patterns of vegetation (woody and ground cover), which provides a basis for within-paddock stratification before soil sampling.

Effect of Application of Liquid Swine Manure on Soil Organic Carbon and Enzyme Activities in Two Contrasting Saskatchewan Soils

&lt;p&gt;Repeated application of animal manure to agricultural fields as a source of plant nutrients has led to questions concerning the impact of this practice on soil organic carbon (C) and biochemical properties, specifically the activity of soil enzymes. There are also some environmental concerns of using livestock manure. The objectives of this study were to determine the effects of repeated applications of liquid swine manure (LSM) on total organic C (TOC), light fraction organic C (LFOC) and on the activity of the soil enzymes (arylsulfatase, alkaline phosphatase and urease) in two contrasting soil-climatic zones and cropping systems in Saskatchewan. Liquid hog manure was applied annually at 37,000 L ha&lt;sup&gt;-1&lt;/sup&gt; and 74,000 L ha&lt;sup&gt;-1&lt;/sup&gt;rates for three years at Melfort (Mollic Cryoboralf) and for four years at Plenty (Typic Boroll) in Saskatchewan, Canada. Soil samples were collected to a depth of 0-15 cm in the spring of 2003 and analyzed for TOC, LFOC and enzyme activities. Annual application (37,000 L ha&lt;sup&gt;-1&lt;/sup&gt;) and larger application made every two years (74,000 L ha&lt;sup&gt;-1&lt;/sup&gt;) of LSM at Melfort increased LFOC, which was attributed to stimulation of plant growth and thus residue inputs, from the nutrients contained within the manure. Applications of LSM at 37,000 L ha&lt;sup&gt;-1&lt;/sup&gt; and 74,000 L ha&lt;sup&gt;-1&lt;/sup&gt; at the Plenty site increased both TOC and LFOC concentration when compared to the control. Soil at the Plenty site is a Typic Boroll of heavy clay texture, which aids in protecting soil organic matter (SOM) from decomposition by soil microorganisms. Melfort was the only site that responded to LSM applications in terms of increased enzyme activity, which may be a result of a shorter application history. The Melfort site also had significantly higher LFOC in the manure treatments compared to the other site and LFOC has been linked to enzyme activity. The results of this study indicate that it may take a long period of time for addition of LSM to produce measurable changes in TOC and LFOC, as the effect from LSM is mainly from the stimulation of plant growth rather than from direct additions of C, and the nutrients contained in the LSM may potentially enhance microbial decomposition. In soils that receive repeated applications of LSM, nutrient loading may contribute to reduced enzyme activity after a period of time.&lt;/p&gt;

Addition of a clay subsoil to a sandy topsoil changes the response of microbial activity to drying and rewetting after residue addition – a model experiment

AbstractThe effect of drying and rewetting (DRW) on C mineralization has been studied extensively but mostly in absence of freshly added residues. But in agricultural soils large amounts of residues can be present after harvest; therefore, the impact of DRW in soil after residue addition is of interest. Further, sandy soils may be ameliorated by adding clay‐rich subsoil which could change the response of microbes to DRW. The aim of this study was to investigate the effect of DRW on microbial activity and growth in soils that were modified by mixing clay subsoil into sandy top soil and wheat residues were added. We conducted an incubation experiment by mixing finely ground wheat residue (20 g kg–1) into top loamy sand soil with clay‐rich subsoil at 0, 5, 10, 20, 30, and 40% (w/w). At each clay addition rate, two moisture treatments were imposed: constantly moist control (CM) at 75% WHC or dry and rewet. Soil respiration was measured continuously, and microbial biomass C (MBC) was determined on day 5 (before drying), when the soil was dried, after 5 d dry, and 5 d after rewetting. In the constantly moist treatment, increasing addition rate of clay subsoil decreased cumulative respiration per g soil, but had no effect on cumulative respiration per g total organic C (TOC), indicating that the lower respiration with clay subsoil was due to the low TOC content of the sand‐clay mixes. Clay subsoil addition did not affect the MBC concentration per g TOC but reduced the concentration of K2SO4 extractable C per g TOC. In the DRW treatment, cumulative respiration per g TOC during the dry phase increased with increasing clay subsoil addition rate. Rewetting of dry soil caused a flush of respiration in all soils but cumulative respiration at the end of the experiment remained lower than in the constantly moist soils. Respiration rates after rewetting were higher than at the corresponding days in constantly moist soils only at clay subsoil addition rates of 20 to 40%. We conclude that in presence of residues, addition of clay subsoil to a sandy top soil improves microbial activity during the dry phase and upon rewetting but has little effect on microbial biomass.