Organic fertilizers are able to bind the ability of soil to absorb water, increase resistance to erosion, improve biodiversity, and increase soil fertility, but will not increase residuals on crop yields so it is safe for the environment and human health. Agricultural waste is a by-product of agriculture that is not utilized or thrown away so that it has no sale value like vegetable waste, rotten fruit, coconut fiber, agricultural waste, fisheries and household waste, which can directly cause environmental pollution, sources disease, and interfering with environmental cleanliness. Rumen cow is a slaughterhouse waste that is not utilized properly, even though the rumen still contains nutrients, microbes and undigested food that can be recycled. The objective of this study was to find out the ratio of the composition of rumen fluid and agricultural waste to the liquid organic fertilizer nutrient content. The analysis method used was 3x3 technique of rumen fluid for each control, namely PI 15% rumen fluid, PII 30% rumen fluid and PIII 45% rumen fluid. The results of the nutrient analysis of organic liquid fertilizer towards agricultural waste and rumen liquid showed that the highest nitrogen in PII is 1.85%, PIII was 1.29% and Pi was 0.68%. The highest phosphorus (P) content in PII was 2.52%, PIII was 2.47% and PI was 1.87% and the highest potassium (K) content was in PII (2.90), PIII was 2.72 and PI was 2.14. According to the results obtained, nutrient analysis of liquid organic fertilizer is in accordance with the quality standards of liquid organic fertilizer regulated in the Agriculture Regulation No. 28/Regulation of the Minister of Agriculture/OT.140/2/200 which stated that liquid organic fertilizer for its N-total content, Phosphorus and Potassium is greater of 2%.