The aim of this research was the identification of biologically active secondary metabolites isolated from rare plant species Pulsatilla patens (L.) Mill. and cultivated Pulastilla vulgaris Mill. (Ranunculaceae). Chromatographic fractionation of the ethanolic extract of the roots of P. patens resulted in the isolation of two known oleanane-type glycosides identified as hederagenin 3-O-β-D-glucopyranoside and hederagenin 3-O-β-D-galactopyranosyl-(1→2)-β-D-glucopyranoside [1]. The structures of the isolated compounds were determined by 1H and 13C-NMR spectroscopy. Chromatographic analysis using GC-MS of the silylated methanolic extract of the leaves of P. vulgaris revealed the presence of carboxylic acids, such as benzoic, caffeic, malic, and succinic acids [2]. Relative composition (%) of the isolated compounds in total ion current (TIC) was calculated on the basis of peak area in the chromatogram. The extracts of Pulsatilla species were evaluated for their antifungal, antimicrobial, antileishmanial and antimalarial activities. Both P. patens and P. vulgaris were active against the fungus Candida glabrata with IC50 values of 9.37 µg/mL [1] and 11 µg/mL.