Total Hg Concentrations Research Articles

Total mercury and methylmercury in the soil and vegetation of a riparian zone along a mercury-impacted reservoir

The Baihua Reservoir (Guizhou Province, Southwest China) has a history of mercury contamination associated with past acetic acid production activities at the Guizhou Organic Chemical Plant (GOCP). Soil and plant samples collected from riparian zones were analyzed for total Hg (THg) and methylmercury (MeHg) using cold vapor atomic fluorescence spectroscopy. The concentrations of THg and MeHg in soil samples were in the range of 109–371 ng g−1 and 0.32–1.80 ng g−1, respectively. Soils in the riparian zones close to the pollution source (the GOCP) presented higher Hg contamination, with relatively light Hg contamination in remote areas. This suggests a decreasing trend of THg concentrations along the riparian zones, with higher concentrations closer to the pollution source. Significant correlations were found between MeHg and soil organic matter (n = 24, p = 0.01). THg concentrations varied 11.3–161 ng g−1 in aboveground areas and 11.3–193 ng g−1 in underground areas. MeHg concentrations ranged from 0.23 to 1.06 ng g−1 in aboveground areas to 0.13–1.51 ng g−1 in the below ground areas. The vegetation studied showed different concentrations of THg and MeHg and can be considered to be impacted by Hg contamination. Different concentrations of total and methyl mercury were found among the different plant species. The high Hg concentrations in soils and vegetation suggests that the ability of Hg to bioaccumulate in riparian plants is affected by plant physiological characteristics and soil mercury concentrations. Although the bioaccumulation factors (BCFs) of the studied plants were low, their transfer factors (TFs) were >1. Our findings suggest that vegetation exhibiting TFs for THg >1 have the potential for phytoextraction in Hg-impacted riparian zones.

Mercury contamination of soil and water media from different illegal artisanal small-scale gold mining operations (galamsey)

Environmental media contamination with mercury, because of illegal artisanal small-scale gold mining (popularly called galamsey), is a major concern in Ghana; yet specific details as to how such contaminations are influenced or distributed across different galamsey operations have been lacking. We monitored mercury levels across nine different galamsey operations (Washing Board, Washing Plant, Anwona, Dig and Wash, Dredging, Underground Abandoned Shaft, Underground Sample Pit, Chamfi and Mill House) in three hotspot assemblies (Tarkwa Nsuaem, Amenfi East and Prestea Huni Valley) of the Western Region of Ghana. Triplicate samples each of background soil, surface water/drainage, slurry/sludge and galamsey waste materials (totaling 160) were obtained and analyzed using Atomic Absorption Spectrophotometer (AAS) to determine total Hg concentrations. From the comparison of mean ranked concentration of mercury, using the Kruskal-Wallis Test, it was realized that the observed differences in ranking was significant for all four environmental media considered. Thus, the poor handling, usage and disposal of mercury from the different galamsey activities did result in elevation of harmful quantities of mercury into the environment. Overall, the highest median value obtained for mercury was recorded at the Mill House galamsey sites and within slurry/sludge medium. This was followed by Chamfi, Washing Board, Washing Plant, Anwona, Dig and Wash and Dredging in descending order, with the Underground Abandoned Shaft and Underground Sample Pit galamsey types recording values below detection limit. In terms of their contribution to mercury contamination to the environment, Mill House, Chamfi, Anwona, Washing Board and Washing Plant galamsey types recorded the highest mean rankings. Overall, key priority information required for influencing reclamation and cleanup policy decisions for mercury, for the many affected wastelands across the country, can be derived from this paper.

Effect of deep Southwestern Subtropical Atlantic Ocean circulation on the biogeochemistry of mercury during the last two glacial/interglacial cycles

During glacial/interglacial cycles, changes in the strength of the Atlantic Meridional Overturning Circulation (AMOC) modified the intermediate and deep-water mass proportions and high latitude productivity in the Atlantic Ocean. These factors influence the distribution and geochemical partitioning of trace metals in the ocean. Mercury is a redox and productivity-sensitive trace metal, making it a potential proxy of paleoenvironmental changes. Therefore, this work examines the effect of Atlantic Ocean circulation changes during the last two glacial/interglacial cycles on the biogeochemistry of Hg. For this, a high-resolution record of the total Hg concentration was determined in core GL-1090 collected from the Southwestern Subtropical Atlantic that represents the last 185 thousand years. During the reported glacial/interglacial cycles, Hg showed a distinct trend throughout Marine Isotope Stages with higher concentrations during periods of enhanced penetration of northern component water into the southwestern Atlantic. This is supported by the similarity of mercury variability with benthic foraminifera δ13C, suggesting a strong influence of deep ocean circulation on the availability and accumulation of this metal in deep-sea sediments. Mercury geochemistry and particle scavenging were correlated with organic matter (OM) input at the core site. We also noted that mercury responded to redox variation in sediment after Termination II, which can be explained by the increase in deep ocean ventilation due to AMOC strengthening. This hypothesis was confirmed by the antiphase behavior of Hg and Total Organic Carbon when compared with Mn/Al ratios and CaCO3. Our work, therefore, allows for a better understanding of the processes leading to long-term mercury removal to sediments.

Total mercury in wild felids occurring in protected areas in the central Brazilian Amazon

ABSTRACT This is the first study to investigate mercury (Hg) contamination in felid species in the Brazilian Amazon. We collected 26 fur samples from wild felids of four species (Puma concolor, Panthera onca, Leopardus pardalis and Leopardus wiedii) occurring in the Mamirauá and Amanã sustainable development reserves, in the state of Amazonas. Samples were from museum specimens, except for five P. onca samples collected from free-living individuals. Total Hg concentrations ranged from 0.12 to 48.1 µg g-1. Concentrations of Hg did not differ significantly between museum specimens and live individuals of P. onca, but varied significantly among species, with significantly higher concentrations for P. onca and L. pardalis, which could be related to factors such as diet and habitat.

Mercury variation and export in trans-Himalayan rivers: Insights from field observations in the Koshi River

Strengthening the research of riverine mercury (Hg) export is of great significance for understanding the regional and global Hg cycle, especially for the data lacking trans-Himalayan rivers. In this study, three systematic sampling campaigns were conducted in the Koshi River Basin (KRB) during the post-monsoon, pre-monsoon and monsoon seasons. Hg speciation and distribution of river water were analyzed among the different seasons for a total of 88 water samples. The total Hg (THg) concentration of surface water in the KRB ranged from 0.64 to 32.96 ng·L−1 with an average of 5.83 ± 6.19 ng·L−1 and decreased in the order of post-monsoon (8.79 ± 7.32 ng·L−1) > monsoon (6.68 ± 6.12 ng·L−1) > pre-monsoon (2.18 ± 1.29 ng·L−1). Particulate Hg (PHg) accounted for 63% of THg on average and had a positive correlation with THg among all the three sampling seasons, indicating that the differences in PHg concentration were likely one of the main factors leading to the seasonal and spatial variations in THg in the KRB surface water. The annual Hg exports and fluxes were estimated to be 339.04 kg and 3.88 μg·m−2·yr−1, respectively. Furthermore, Hg export from the KRB had significant seasonal variation and decreased in the order of monsoon (259.47 kg) > post-monsoon (61.18 kg) > winter (9.31 kg) > pre-monsoon (9.08 kg), and this pattern was mainly related to seasonal changes in river runoff. The annual Hg export is projected to increase in the future, especially in the post-monsoon season. Therefore, more attention should be paid to river runoff observations and riverine Hg research for water resources management in the Himalaya.

Litterfall Hg deposition to an oak forest soil from southwestern Europe

Litterfall constitutes one of the main vectors for mercury (Hg) transfer to forested ecosystems, so we studied the deposition of Hg through senescent vegetation (oak leaves, twigs and miscellaneous) in a deciduous forest plot of Southwest Europe dominated by Quercus robur in 2015 and 2016. Total Hg concentrations increased in the following order: bole wood (1.4 μg kg−1) < bark (8.3 μg kg−1) < twigs (12.2 μg kg−1) < miscellaneous (36.0 μg kg−1) < oak leaves (39.3 μg kg−1) < mineral soil (42.4 μg kg−1) < Oi horizons (48.7 μg kg−1) < Oe + Oa horizons (71.6 μg kg−1). Mercury accumulation rates in oak leaves during the growing season were 0.15–0.18 μg kg−1 day−1. Mercury deposition fluxes were 26 and 21 μg m−2 yr−1 for 2015 and 2016, respectively, with oak leaves being the fraction that contributed the most. Mercury determination in litterfall sorted biomass fractions lead to a more accurate estimation of the total annual Hg deposition fluxes through litterfall. Higher Hg content was obtained for organic horizons (average of 60.2 μg kg−1) than for mineral soil (mean of 42.4 μg kg−1), but the soil Hg pool was higher in the latter. The results confirmed the necessity of taking into account the Hg pool in the deeper mineral soil layers as they accumulate substantial quantities of Hg associated to organic C and Al compounds, preventing its mobilization to other compartments of the terrestrial ecosystems.

Two Decades of Mercury Concentrations in Barents Sea Polar Bears (Ursus maritimus) in Relation to Dietary Carbon, Sulfur, and Nitrogen.

Temporal trends of total mercury (THg) were examined in female polar bear (Ursus maritimus) hair (n = 199) from the Barents Sea in 1995-2016. In addition, hair values of stable isotopes (n = 190-197) of carbon (δ13C), sulfur (δ34S), and nitrogen (δ15N) and information on breeding status, body condition, and age were obtained. Stable isotope values of carbon and sulfur reflect dietary source (e.g., marine vs terrestrial) and the nitrogen trophic level. Values for δ13C and δ34S declined by -1.62 and -1.18‰ over the time of the study period, respectively, while values for δ15N showed no trend. Total Hg concentrations were positively related to both δ13C and δ34S. Yearly median THg concentrations ranged from 1.61 to 2.75 μg/g and increased nonlinearly by 0.86 μg/g in total over the study. Correcting THg concentrations for stable isotope values of carbon and sulfur and additionally breeding status and age slightly accelerated the increase in THg concentrations; however, confidence intervals of the raw THg trend and the corrected THg trend had substantial overlap. The rise in THg concentrations in the polar bear food web was possibly related to climate-related re-emissions of previously stored Hg from thawing sea-ice, glaciers, and permafrost.

Assessing oxidative stress in Steller sea lions (Eumetopias jubatus): Associations with mercury and selenium concentrations

Selenium (Se) bioavailability is required for synthesis and function of essential Se-dependent antioxidants, including the enzyme glutathione peroxidase (GPx). Strong interactions between monomethyl mercury and Se impair the critical antioxidant role of Se. Approximately 20% of Steller sea lion (Eumetopias jubatus, SSL) pups sampled in the western Aleutian Islands, Alaska, had total Hg concentrations ([THg]) measured in hair and whole blood above thresholds of concern for adverse physiologic effects in pinnipeds. Importantly, low molar ratios of TSe:THg, in some cases < 1 in several tissues (hair, liver, pelt, muscle, spleen, intestine, heart, lungs, brain) were documented for one SSL pup with [THg] above threshold of concern, which may lead to antioxidant deficiency. Our aim with this study was to evaluate the relationship between circulating [THg], [MeHg+], [TSe] and TSe:THg molar ratio status relative to oxidative stress and antioxidants measured during general anesthesia in free-ranging SSL. We captured, anesthetized and sampled newborn SSL pups at rookeries located in the Aleutian Islands or Gulf of Alaska. Biomarkers analyzed for oxidative stress included 4-hydroxynenonal and thiobarbituric acid reactive substances (4-HNE and TBARS, respectively, lipid peroxidation), protein carbonyl content (PCC, protein oxidation), and GPx activity as a key indicator for Se-dependent antioxidant defense levels. We found a negative association between TBARS and [TSe], and SSL with low [TSe] had higher concentrations of 4-HNE than those with intermediate [TSe]. These results suggest that SSL with lower [TSe] experience increased lipid peroxidation potentially associated with [THg] status.

Effects of seafood consumption on mercury exposure in Norwegian pregnant women: A randomized controlled trial

BackgroundSeafood provides nutrients that are important for optimal development of the unborn child. However, seafood is also a source of contaminants including mercury (Hg) and methylmercury (MeHg) that may have adverse effects on neurodevelopment of the fetus. Humans are predominantly exposed to MeHg through seafood consumption, however, levels of MeHg vary considerably between species. ObjectivesInvestigate, in a randomized controlled trial (RCT) during pregnancy, if an increased intake of Atlantic cod (Gadus morhua), a fish species with relatively low levels of MeHg contamination, influences total hair Hg (THHg) concentrations in humans. MethodsPregnant women (n = 137) were enrolled in the RCT “Mommy’s Food” (2016–2017), which was designed to increase iodine status. Participants were randomly assigned to intervention (400 g of cod fillets per week) or control (continued habitual diet) groups for 16 weeks (gestational week 20–36). THHg concentrations were measured at baseline and post-intervention using thermal decomposition, amalgamation, and atomic absorption spectrophotometry (US EPA method 7473). The trial is registered in ClinicalTrials.gov, NCT02610959. ResultsPost-intervention, the intervention group had median (inter-quartile range) THHg concentrations of 554 (392–805) µg/kg, and the control group 485 (341–740) µg/kg (p = 0.186). When adjusting for baseline THHg concentrations, there was a significant difference between the groups in those participants with baseline THHg concentrations below 534 µg/kg. Post-intervention, 8% of the study population exceeded the US EPA reference dose in hair (1,000 µg/kg) (intervention group: n = 6, control group: n = 4). ConclusionTHHg concentrations were generally low in both study groups of pregnant women, despite the relatively high seafood intake. While the intervention with 400 g of cod per week slightly increased THHg concentrations, it did not lead to an increase in number of subjects exceeding the US EPA reference dose; a dose level at which no adverse effects are expected to occur over a period of lifetime exposure.

Main drivers of mercury levels in Southern Ocean lantern fish Myctophidae

Myctophids are the most abundant fish group in the Southern Ocean pelagic ecosystem and are an important link in the Antarctic marine food web. Due to their major ecological role, evaluating the level of mercury (Hg) contamination in myctophids is important as a step towards understanding the trophic pathway of this contaminant. The concentrations of total Hg were determined in muscle, gill, heart and liver tissue of 9 myctophid species to quantify tissue partitioning variability between species. Organic Hg concentration and proportion in muscle was also determined. Hg concentrations were higher in the liver and heart than in muscle and gills, but the proportion of organic Hg was almost 100% in muscle, indicating that the main uptake route for Hg is through the diet. Most of the species analysed have similar vertical and horizontal distributions, and similar feeding modes and prey. Geographical and temporal variability of Hg concentrations was examined using samples from 3 different sampling cruise (2007/08, 2015/16 and 2016/17) and 2 locations (South Georgia and South Orkneys Islands). Our results appear to indicate a decreasing trend in Hg contamination over the last decade, particularly gill tissue, which is in agreement with a previous study on squid from the same region. There was no significant variability in Hg concentration between the different sampling locations. Hg levels were consistent with values reported previously for myctophids around the world, indicating low global-scale geographic variability. A positive relationship between fish size and Hg concentration was found for most species, with the exception of Electrona antarctica females, which may be explained through Hg elimination by egg laying. We estimate that myctophids collectively comprise a Southern Ocean mercury ‘reserve’ of ≈1.82 metric tonnes.

Sulfur-modified biochar as a soil amendment to stabilize mercury pollution: An accelerated simulation of long-term aging effects

The stability of mercury (Hg) contamination in soil environments can change over time. This has implications for agricultural sites under long-term management after in situ treatment involving soil amendments. In this study, rice husk biochar (RHB) and sulfur modified rice husk biochar (SRHB) were synthesized and applied (dosage = 5% dry wt.) to a Hg polluted agricultural soil collected from Guizhou province, Southern China (soil total Hg content = 28.3 mg/kg; C = 2%; and, S = 0.1%). The long-term stabilization effectiveness of the soil treatments was evaluated by a combined approach involving: (i) accelerated aging for 104 simulated years; (ii) soil extraction as a proxy for plant uptake; and, (iii) sequential extraction to identify Hg fractions. The SRHB amendment raised the soil’s total S content by approximately an order of magnitude (to 0.9%), which remained at a generally constant level throughout the simulation. The initial pH levels for the untreated and treated soils were alkaline and remained between 7.0 and 7.5 for the first 50 years of simulated aging, before decreasing as the simulation time increased further. The pH of the SRHB treated soils did not drop below that of untreated soils during the simulation. Soil extraction tests with 0.1 M HCl solution indicated that RHB and SRHB treatments could effectively immobilize the Hg in soil for at least 50 and 75 simulated years, respectively. At simulated year 50, the amount of Hg extracted from RHB and SRHB treated soils was <200 ng/L and <100 ng/L, respectively. Thus, showing SRHB to be a particularly promising remedial option. The soil Hg was mostly associated with the stable sequential extraction fractions (F3-5). By the end of the simulation, the F5 fraction for SRHB and RHB treated soils reduced by 44.6%, and 42.0%, respectively, whereas the F4 fraction increased by >400% in both cases. In summary, SRHB may provide long-lasting Hg stabilization at contaminated sites. Therefore, further research toward the development of this stabilization technology is warranted.

Mercury distribution and transfer in sediment-mangrove system in urban mangroves of fast-developing coastal region, Southern China

In urban mangroves affected by surrounding urbanization and anthropogenic activities, the fate of mercury (Hg) in sediment-mangrove system remains unclear. In this study, four urban mangroves with different urban functional zonings in fast-developing Shenzhen of China were selected: Shajing mangrove (SJM) and Xixiang mangrove (XXM) featured with industry district, Futian mangrove (FTM) and Baguang mangrove (BGM) featured with central business district and ecological preserve, respectively. The distributions of Hg in sediment, iron plaque (IP), and Kandelia obovata were determined. The mean concentrations of total Hg in mangrove sediments were SJM > XXM, FTM > BGM. Higher ecological risk was also detected in SJM based on geo-accumulation index, potential ecological risk factor, and contamination factor. The speciation analysis of Hg in non-rhizosphere sediments showed that residual, oxidizable, and volatile Hg acted as dominant fractions in urban mangroves, with volatile Hg to be important for Hg bioaccumulation in K. obovata. IP acted as a natural barrier to limit transfer of Hg from sediment to K. obovate due to lower Hg concentrations in IP compared to that in sediment. In terms of the total and bioavailable Hg, translocation factors (TF) of Hg in mangroves featured with different urban functions were: TFtotal: BGM (0.23) ≈ FTM (0.23) > SJM (0.15) ≈ XXM (0.14); TFavail: BGM (0.81) ≈ FTM (0.74) > SJM (0.40) ≈ XXM (0.37). The reduced TFs of Hg were important for protecting K. obovata from heavy Hg pollution in urban mangroves featured with industry district.

Neuropsychological Effects of Mercury Exposure in Children and Adolescents of the Amazon Region, Brazil

BackgroundStudies with children exposed to methylmercury (MeHg) through fish consumption in the Brazilian Amazon region report that the high levels of hair Hg are associated with significant decreases in intelligence, memory, attention, and visuospatial processing. ObjectiveTo investigate the relationship between mercury exposure and neuropsychological functions in riverside communities of the Brazilian Amazon. Method263 participants aged 6 to 14 years old were assessed, from resettlement regions, near the Madeira river, Rondônia, Brazil. To assess the neuropsychological functions we used the following instruments: intelligence (WASI), working memory (Corsi Block-Tapping Task and Digit Span), verbal fluency (Word Generation - NEPSY II), inhibitory control (Inhibition Errors - NEPSY II), shifting (Trail Making Test) and manual motor dexterity (Grooved PegBoard Test). Socioeconomic status was obtained through household surveys. Total Hg levels were quantified hair samples (Total HgH) collected from the occipital region of the scalp and analyzed by Cold Vapor Atomic Absorption Spectrometry. ResultsThe group in the upper quartile of Total HgH levels presented lower scores on the tasks that assessed estimated IQ, visuospatial working memory, semantic knowledge and phonological verbal fluency, when compared to the group in the lower quartile level. A regression analysis controlled for age, sex, and maternal education showed that for each increase of 10 μg/g of Total HgH, there was a decrease around half standard deviation in Verbal IQ, estimated IQ scores, semantic knowledge, phonological verbal fluency and for verbal and visuospatial working memory. ConclusionsHigh concentrations of Total Hg in hair were associated with a lower performance in neuropsychological functions tests. The results show that environmental exposure to Hg is associated to children and adolescents’ lower neuropsychological performance in the riverine and resettled areas of the Brazilian Amazon region.

Mercury contamination status of rice cropping system in Pakistan and associated health risks

Rice is a known bioaccumulator of methylmercury (MeHg). Rice consumption may be the primary pathway of MeHg exposure in certain mercury (Hg)-contaminated areas of the world. Pakistan is the 4th-largest rice exporter in the world after India, Thailand, and Vietnam. This study aimed to evaluate the Hg contamination status of rice from Pakistan and the health risks associated with Hg exposure through its consumption. 500 rice grain samples were collected from two major rice-growing provinces, Punjab and Sindh, which contain 92% of Pakistan’s rice cultivation area. Analysis of polished rice showed mean total Hg (THg) concentration of 4.51 ng.g−1, while MeHg concentrations of selected samples averaged 3.71 ng.g−1. Only 2% of the samples exceeded the permissible limit of 20 ng.g−1. Samples collected from Punjab showed higher Hg contents than those from Sindh, possibly due to higher rates of urbanization and industrialization. Rice samples collected from areas near brick-making kilns had the highest Hg concentrations due to emissions from the low-quality coal burned. THg and MeHg contents varied by up to five and fourfold, respectively, between point and non-point Hg pollution sites. Moreover, the %Hg as MeHg in rice did not differ significantly between point and non-point Hg sources. Health risk was assessed by calculating a mean probable daily intake, revealing that Hg intake through rice consumption is within the safe limits recommended by the World Health Organization. However, rice intake may be a substantive pathway of MeHg exposure because fish, which are another major source of Hg, are consumed in Pakistan at some of the world’s lowest rates. This study provides fundamental data for further understanding of the global issue of Hg contamination of rice and its related health risks. Furthermore, the current study suggests there is a need to conduct further research in rice-growing areas at the regional level.

Concentration and isotopic composition of mercury in a blackwater river affected by extreme flooding events

AbstractTorrential rain and extreme flooding caused by Atlantic hurricanes mobilize a large pool of organic matter (OM) from coastal forested watersheds in the southeastern United States. However, the mobilization of toxic metals such as mercury (Hg) that are associated with this vast pool of OM are rarely measured. This study aims to assess the variations of total Hg (THg) and methylmercury (MeHg) levels and the isotopic compositions of Hg in a blackwater river (Waccamaw River, SC, U.S.A.) during two recent extreme flooding events induced by Hurricane Joaquin (October 2015) and Hurricane Matthew (October 2016). We show that extreme flooding considerably increased filtered THg and MeHg concentrations associated with aromatic dissolved organic matter. During a 2‐month sampling window each year (October–November), we estimate that about 27% (2015) and 78% (2016) of the average amount of Hg deposited atmospherically during these 2 months was exported via the river. The isotopic composition of Hg in the river waters was changed only slightly by the substantial inputs of runoff from surrounding landscapes, in which mass‐dependent fractionation (as δ202Hg) decreased from −1.47 to −1.67‰ and mass‐independent fractionation (as ∆199Hg) decreased from −0.15 to −0.37‰. The slight variations in Hg isotopic composition during such extreme flooding events imply that sources of Hg in the river are nearly unchanged even under the very high wet deposition of Hg derived from the intensive rainfall. The majority of Hg exported by the river (74–85%) is estimated to have been derived from dry deposition to the watersheds. An increase in frequency and intensity of Atlantic hurricanes is expected in the next few decades due to further warming of ocean surface waters. We predict that increased hurricanes will mobilize more dry‐deposited Hg and in situ produced MeHg from these coastal watersheds where MeHg can be extensively bioaccumulated and biomagnified in the downstream aquatic food webs.

Distribution and source identification of potentially toxic elements in agricultural soils through high-resolution sampling☆

Accumulation of potentially toxic elements (PTEs) in agricultural soils has become an increasingly global issue driven by industrialization. A credible knowledge of their distribution and diverse sources in soils is essential to developing effective measures of identifying accumulation of PTEs in rural and periurban environments. However, the assessment of PTE pollution levels and discrimination of anthropogenic and natural sources remain a serious challenge. In China, most studies are focused on highly industrialized and/or urbanized regions, while limited attention has been given to agricultural areas where diffuse source contamination prevails. Therefore, a large dataset of 5207 surface soil samples (1 × 1 km) and 1311 subsoil samples (2 × 2 km) were collected from an agriculturally dominated region in eastern China. Soil total concentrations of As, Cd, Cr, Cu, Hg, Ni, Pb and Zn were analyzed along with additional edaphic variables relevant to PTE accumulation in soils (e.g., pH, SOC). Concentrations of Cr, Ni, Pb and Zn for all sites were lower than those of the risk-screening values. However, elevated concentrations of Cd and Hg observed in surface soils were associated with anthropogenic activities. Land use pattern had a significant impact on the spatial variation of PTEs. Elevated levels of Cd were uniquely associated with high phosphorus and soil organic matter (SOM) contents, and elevated Hg was associated with coal-fired power plants and historical application of fertilizer and Hg-containing pesticides. The data presented herein indicated that geogenic process likely caused a net depletion of As, Cr, Cu, Ni, Pb and Zn in topsoil, despite surface deposition from anthropogenic sources. The result of this study revealed that using subsoil concentrations of PTEs to establish background or reference concentrations might lead to an erroneous assessment of pollution levels in surface soils.

Mercury bioaccumulation in freshwater fishes of the Chesapeake Bay watershed.

Chemical contaminants are a threat to the Chesapeake Bay watershed, with mercury (Hg) among the most prevalent causes of impairment. Despite this, large-scale patterns of Hg concentrations, and the potential risks to fish, wildlife, and humans across the watershed, are poorly understood. We compiled fish Hg data from state monitoring programs and recent research efforts to address this knowledge gap and provide a comprehensive assessment of fish Hg concentrations in the watershed's freshwater habitats. The resulting dataset consisted of nearly 8000 total Hg (THg) concentrations from 600 locations. Across the watershed, fish THg concentrations spanned a 44-fold range, with mean concentrations varying by 2.6- and 8.8-fold among major sub-watersheds and individual 8-digit hydrological units, respectively. Although, mean THg concentrations tended to be moderate, fish frequently exceeded benchmarks for potential adverse health effects, with 45, 48, and 36% of all samples exceeding benchmarks for human, avian piscivore, and fish risk, respectively. Importantly, the percentage of fish exceeding these benchmarks was not uniform among species or locations. The variation in fish THg concentrations among species and sites highlights the roles of waterbody, landscape, and ecological processes in shaping broad patterns in Hg risk across the watershed. We outline an integrated Hg monitoring program that could identify key factors influencing Hg concentrations across the watershed and facilitate the implementation of management strategies to mitigate the risks posed by Hg.

Latitudinal, sex and inter-specific differences in mercury and other trace metal concentrations in Adélie and Emperor penguins in the Ross Sea, Antarctica

We sought to determine mercury (Hg) and other trace metal concentrations in Adélie (Pygoscelis adeliae) and emperor penguin (Aptenodytes forsteri) breast feathers from the Ross Sea, Antarctica, and relate those concentrations to the trophic position and the habitats in which each of these species forage. Adélie penguin feathers from the southern Ross Sea colonies were higher in Hg than those sampled further north in the Ross Sea, potentially due to greater exposure to local sources, such as volcanism. Female Adélie penguins had lower feather total Hg concentrations than males. This may reflect female penguin's capacity to eliminate Hg through the egg development and laying process, or the larger and/or older prey items that male birds can consume, reflected by their higher trophic position. Emperor penguins have higher Hg concentrations than Adélie penguins which is also partially explained by Adélie penguins feeding at lower trophic levels than emperor penguins.

Dried Blood Spot Sampling of Landlocked Arctic Char (Salvelinus alpinus) for Estimating Mercury Exposure and Stable Carbon Isotope Fingerprinting of Essential Amino Acids.

Dried blood spots (DBS), created by applying and drying a whole blood sample onto filter paper, provide a simple and minimally invasive procedure for collecting, transporting, and storing blood. Because DBS are ideal for use in field and resource-limited settings, we aimed to develop a simple and accurate DBS-based approach for assessing mercury (Hg) exposure and dietary carbon sources for landlocked Arctic char, a sentinel fish species in the Arctic. We collected liquid whole blood (from the caudal vein), muscle, liver, and brains of Arctic char (n = 36) from 8 lakes spanning a Hg gradient in the Canadian High Arctic. We measured total Hg concentrations ([THg]) of field-prepared DBS and Arctic char tissues. Across a considerable range, [THg] of DBS (0.04-3.38 μg/g wet wt) were highly correlated with [THg] of all tissues (r2 range = 0.928-0.996). We also analyzed the compound-specific carbon isotope ratios (expressed as δ13 C values) of essential amino acids (EAAs) isolated from DBS, liquid whole blood, and muscle. The δ13 C values of 5 EAAs (δ13 CEAAs ; isoleucine [Ile], leucine [Leu], phenylalanine [Phe], valine [Val], and threonine [Thr]) from DBS were highly correlated with δ13 CEAAs of liquid whole blood (r2 range = 0.693-0.895) and muscle (r2 range = 0.642-0.881). The patterns of δ13 CEAAs of landlocked Arctic char were remarkably consistent across sample types and indicate that EAAs are most likely of algal origin. Because a small volume of blood (~50 µL) dried on filter paper can be used to determine Hg exposure levels of various tissues and to fingerprint carbon sources, DBS sampling may decrease the burdens of research and may be developed as a nonlethal sampling technique. Environ Toxicol Chem 2020;39:893-903. © 2020 SETAC.

Testing the Use of Standardized Laboratory Tests to Infer Hg Bioaccumulation in Indigenous Benthic Organisms of Lake Maggiore (NW Italy)

The chronic toxicity of mercury essentially derives from its strong tendency to biomagnify along food webs. For this reason, the European regulatory framework establishes an environmental quality standard for Hg based on the total Hg concentration in prey fish to protect top predators. A considerable part of the Hg burden of prey fish can come from the ingestion of benthic organisms that, in the presence of contaminated sediments, may remobilize substantial amounts of Hg towards the pelagic food webs. The present study evaluated whether Hg accumulation in assemblages of indigenous chironomids and oligochaetes could be predicted using standardized laboratory bioaccumulation tests with Chironomus riparius and Lumbriculus variegatus. Indigenous chironomids and oligochaetes were recovered at different sites in a lake suffering from legacy Hg pollution and analyzed for total Hg content. Sediment aliquots from the same sites were used to assess Hg bioaccumulation using laboratory-reared C. riparius and L. variegatus. Mercury concentrations in indigenous versus laboratory organisms showed a good correlation (p &lt; 0.05; Spearman correlation test) only in the case of C. riparius versus indigenous chironomids, suggesting the possibility of using linear regressions to predict Hg accumulation by these benthic invertebrates. Further research needs and caveats as to the applicability of the present results to other aquatic systems are identified and discussed.