AbstractThis aim of this work was to investigate the various properties of Origanum munzurense Kit Tan & Sorger that are previously unknown. In this context, chemical compound, antioxidant activity, total polyphenol content, mineral and trace element composition, antibacterial activity and genotoxicity effects of its extracts and essential oil were widely analysed. The extracts and essential oil of Origanum munzurense were obtained by three different extraction methods. All contents of extracts and essential oil were analysed by GC‐MS and evaluated according to mass spectral libraries of GC‐MS. The identified compounds were evaluated according to literature to put forward their benefits on human health. The phenolic content analysis of Origanum munzurense was performed using the Folin‐Ciocalteu method. 178.59, 184.20, 231.17, 319.28 and 1051.94 mg GAE/100 g dry weight were determined for methanol, water, acetonitrile, ethanol and subcritical water extracts, respectively. DPPH and CUPRAC methods were used to determine antioxidant activity. The highest half‐maximal inhibitory concentration and Trolox equivalent antioxidant capacity values were found as 0.110 mg mL−1 and 2.197 using water and tetrahydrofuran extracts, respectively. A total of 21 mineral and trace elements were detected in the ICP‐MS analysis of O munzurense using the wet digestion procedure. The effects of the extracts and essential oil on Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and S aureus were examined using disc diffusion and microdilution methods. Essential oil exhibited the highest antibacterial activity. The essential oil was found to cause less DNA damage using the comet assay in Hep G2 cells compared with tetrahydrofuran extract.