Content Of Total Phenolic Compounds Research Articles

Physicochemical Characteristics, Total Phenolic Content and Free Radical Scavenging Activity of Apple (Malus Domestica) Peel Powder

Apple peel is considered as a waste product in many fruit industries but it is a noteworthy source of nutrients and phytochemicals, particularly polyphenols which have the ability to scavenge free radicals. Objectives: To study the physicochemical properties of apple (Malus domestica) peel powder, as well as its TPC and free radical scavenging activities. Methods: Proximate analysis of the apple peels powder was carried out. Total polyphenol content of apple peel powder was determined. The total content of phenolic compounds was expressed as gallic acid equivalent, i.e. mg GAE/100g of extract dry weight of sample. Results: The results of physicochemical characteristics moisture (7.65±0.88%), ash (2.50±0.35%), fat (1.18±0.02%), fiber (15.25±1.03%), protein (2.79±0.05%), carbohydrates (71.0±2.30%) and energy were 312±4.70 Kcal/100g. The total phenolic contents in the methanolic extract of apple peel powder were 320±5.4 mg GAE/100g while in H2O extract 201±4.20 mg/100g. The findings of the apple peel powder's methanolic extract's capacity to scavenge free radicals varied from 25.40±1.30—69.2±3.80%, while those of the water extract were 14.30±1.05—45.62±1.90% and BHT were 17.8±1.15—51.62±2.15% at concentration 20-100 µg/ml. Conclusions: The outcomes showed that both apple peel powder extracts had promising total polyphenols and have strong free radical scavenging activity. These findings suggest that the apple peel powder act as robust naturally occurring antioxidants and may be employed as a preventative therapy for several oxidative stress-related degenerative disorders.

Phytochemical and biological investigation of leaf extracts of Phlomis crinita Cav. from northern Algeria

Phlomis crinita Cav., is a Lamiaceae very widespread in the North-West of Algeria (Chlef), but very little studied on the chemical and pharmacological levels; for this, a phytochemical screening, then a dosage of total phenolic compounds (TPC), total flavonoids (TFL) and condensed tannins (TCN) obtained by (02) methods (reflux and ultrasonic bath), followed by an evaluation of antibacterial activities and antioxidants, were carried out on (03) fractions: chloroform, ethyl acetate and n-butanol. The n-butanol extract (reflux) gives the best contents of total phenolic compounds (TTPC= 15.96 ± 0.09 ug Eq. Catechin / mg of extract), The acetate ethyl extract (reflux) gives the best contents of total flavonoids (TTFL = 33.98 ± 4.98 ug Eq. Rutin / mg of extract), and the chloroform extract (reflux) is richer on condensed tannins (TTCN= 757.67 ± 14.32 ug Eq. Catechin / mg of extract). The antibacterial activity of the extracts was slightly positive against Pseudomonas aeruginosa and Staphylococcus aureus. On the other hand, no antibacterial activity was observed against strains of Escherichia coli. The chloroform and n-butanol (reflux) extracts showed a strong ability to scavenge the DPPH radical comparable to that of ascorbic acid (IC50 Chloroform Ext. = 79.99 ± 1.48 ug/ml; IC50 n-Butanol Ext. = 94.96 ± 1.70 ug/ml and IC50 Ethyl Acetate Ext. = 588.68 ± 10.88 ug/ml versus IC50vit C = 55.26 ± 1.01 ug/ml).

떡 재료 식물인 떡쑥, 모시, 쑥 및 절굿대 추출물의 생리활성 효과

This study investigated the physiological activity of cottonweed, ramie, mugwort, and globe thistle as material for making Tteok and determined the total phenolic compound and flavonoid contents, and the DPPH and nitrite radical scavenging activities using different extraction methods (hot water, ethanol, and methanol). The amount of total phenolic compounds in the mugwort material increased from 61.0-214.2 mg/L at a concentration of 1,000 mg/L irrespective of the standard substance and extraction method. The total content of flavonoids was the highest in mugwort compared to the other Tteok materials and the extraction methods of hot water, methanol, and ethanol resulted in 29.1, 17.2, and 13.8 mg/L flavonoid content, respectively. At the extracting concentration of 1,000 mg/L, the DPPH radical scavenging activity of the extracts from globe thistle, cotton weed, and mugwort was not significantly different at 76.7-77.0 mg/L, while ramie showed the lowest activity at 70.7 mg/L. When the extracting concentration was 1,000 mg/L, the nitrite radical scavenging activity of hot water and ethanol mugwort extracts increased the most by 25.8% and 29.0%, respectively. However, that of the methanol extract of globe thistle decreased by 21.0%. These results suggest that making Tteok by use may provide useful information.

Nanoparticulate Fertilizers Increase Nutrient Absorption Efficiency and Agro-Physiological Properties of Lettuce Plant

The extensive use of chemical fertilizers is responsible for numerous environmental problems including low food quality, soil degradation, and toxicity to beneficial living organisms in the soil. Nano-fertilizers (NFs) application may be a promising solution for combat these challenges. The current study focused on the efficiency of applying small amounts of NFs incorporated with conventional nitrogen, phosphorus, and potassium (NPK) fertilizers to reduce the quantities of conventional fertilizers (CFs) in lettuce cultivated in sandy soil. This study evaluated the effect of these incorporations on plant growth, yield, phytochemical accumulation, leaf nutrient, and leaf nitrate. A pot experiment was conducted during the winter seasons of 2020/2021 and 2021/2022 using the following treatments: CF100: 100% CFs, CF75NF25: 75% CFs + 25% NFs, CF50NF50: 50% CFs + 50% NFs, CF25NF75: 25% CFs + 75% NFs, and NF100: 100% NFs (=10% of CFs). Our findings displayed that the CF75NF25 and CF50NF50 treatments recorded the highest plant growth parameter values (plant length, root length, number of leaves, and fresh and dry biomass). The maximum of chlorophyll fluorescence measurements (photosystem II efficiency) were obtained in plants fertilized with CF75NF25, followed by CF50NF50 and CF100. The improvement ratios of photosynthetic pigments (Chlorophyll (Chl) a, b, and total) for CF75NF25 were 23.77, 50, and 23.72% in the first season and 10.10, 51.0, and 24.90% in the second season for Chl a, b, and total, respectively, as compared with the CF100 treatment. A similar tendency was observed for the CF50NF50 treatment. Generally, CF75NF25 significantly raised the content of total phenolic compounds (TPC), total flavonoid content (TFC), and antioxidant activity (AOA) in lettuce plants by 36.09, 47.82, and 40.16% in the first season and by 30.39, 37.53, and 32.43% in the second season, respectively, compared with CF100. In addition, the levels of leaf nutrient content and uptake of N, P, and K were significantly higher in plants fertilized with CF75NF25 compared to the other treatments, whereas CF25NF75 had the lowest values among the different treatments across both seasons for most of the tested traits. The nitrate content in lettuce leaves (NO3−) for both seasons was lower than the acceptable level for human consumption. These results indicate that incorporating a low concentration of NFs into CFs could be a promising strategy to reduce the amount of CFs to 75% or 50% of lettuce NPK requirements without significant adverse effects on the growth and productivity of lettuce plants cultivated in sandy soil.

The impact of spring wheat species and sowing density on soil biochemical properties, content of secondary plant metabolites and the presence of Oulema ssp.

The physical and chemical properties of the soil are important factors influencing the yield of crops. One of the agrotechnical factors influencing the biochemical properties of soil is sowing density. It affects the yield components, light, moisture and thermal conditions in the canopy and the pressure of pests. Secondary metabolites, many of which are known to act as a defense mechanism against insects, are of importance in the interaction between the crop and abiotic and biotic factors of the habitat. To the best of our knowledge, the studies conducted so far do not sufficiently reveal the impacts of the wheat species and the sowing density, together with the biochemical properties of the soil, on the accumulation of bioactive ingredients in the crop plants, and the subsequent impacts on the occurrence of phytophagic entomofauna in various management systems. Explaining these processes creates an opportunity for more sustainable development of agriculture. The study aimed to determine the effect of wheat species and sowing density on the biochemical properties of the soil, concentrations of biologically active compounds in the plant and the occurrence of insect pests in organic (OPS) and conventional (CPS) production systems. The research was conducted on spring wheat species (Indian dwarf wheat-Triticum sphaerococcum Percival and Persian wheat-Triticum persicum Vavilov) grown in OPS and CPS at sowing densities 400, 500, 600 (seeds m-2). The following analyzes were performed: (i) soil analysis: the activity of catalases (CAT), dehydrogenases (DEH), peroxidases (PER); (ii) plant analysis: total phenolic compounds (TP), chlorogenic acid (CA), antioxidant capacity (FRAP); (iii) entomological analysis of the number of insects-Oulema spp. adults and larvae. Performing analyzes in such a wide (interdisciplinary) scope will allow for a comprehensive understanding of the soil-plant-insect biological transformation evaluation. Our results showed that an increase in soil enzyme activity caused a decrease in TP contents in the wheat grown the OPS. Despite this, both the content of TP and the anti-oxidative activity of the ferric reducing ability of plasma (FRAP) were higher in these wheats. Bioactive compound contents and FRAP were most favoured by the lowest sowing density. Regardless of the production system, the occurrence of the Oulema spp. adults on T. sphaerococcum was the lowest at a sowing density of 500 seeds m-2. The occurrence of this pest's larvae was lowest at a sowing density of 400 seeds m-2. Research on bioactive compounds in plants, biochemical properties of soil and the occurrence of pests make it possible to comprehensively assess the impact of the sowing density of ancient wheat in the ecological and conventional production system, which is necessary for the development of environmentally sustainable agriculture.

Productivity, photosynthetic light-use efficiency, nitrogen metabolism and nutritional quality of C4 halophyte Portulaca oleracea L. grown indoors under different light intensities and durations.

Portulaca oleracea L. (known as purslane), is a nutritious facultative C4 halophyte. Recently, it has been successfully grown indoors under LED lightings by our team. However, basic understanding about the impacts of light on purslanes are lacking. This study aimed to investigate the effects of light intensity and duration on productivity, photosynthetic light use efficiency, nitrogen metabolism and nutritional quality of indoor grown purslanes. All plants were grown in 10% artificial seawater hydroponically under different photosynthetic photon flux densities (PPFDs) and durations and thus different daily light integrals (DLI). They are, L1 (240 µmol photon m-2 s-1, 12h, DLI = 10.368mol m-2 day-1); L2 (320 µmol photon m-2 s-1, 18h, DLI = 20.736mol m-2 day-1); L3 (240 µmol photon m-2 s-1, 24h, DLI = 20.736mol m-2 day-1); L4 (480 µmol photon m-2 s-1, 12h, DLI = 20.736mol m-2 day-1), respectively. Compared to L1, higher DLI promoted root and shoot growth and thus increased shoot productivity by 2.63-,1.96-, 3.83-folds, respectively for purslane grown under L2, L3, L4. However, under the same DLI, L3 plants (continuous light, CL) had significantly lower shoot and root productivities compared those with higher PPFDs but shorter durations (L2 and L4). While all plants had similar total chlorophyll and carotenoid concentrations, CL (L3) plants had significantly lower light use efficiency (Fv/Fm ratio), electron transport rate, effective quantum yield of PSII, photochemical- and non-photochemical quenching. Compared to L1, higher DLI with higher PPFDs (L2 and L4) increased leaf maximum nitrate reductase activity while longer durations increased leaf concentrations and total reduced nitrogen. There were no significant differences in leaf total soluble protein, total soluble sugar and total ascorbic acid concentrations in both leaf and stem regardless of light conditions. However, L2 plants had the highest leaf proline concentration but leaf total phenolic compounds concentration was higher in L3 plants instead. Generally, L2 plants had the highest dietary minerals such as K, Ca, Mg and Fe among the four different light conditions. Overall, L2 condition is the most suitable lighting strategy in enhancing productivity and nutritional quality of purslane.

Influence of Different Precursors on Content of Polyphenols in Camellia sinensis In Vitro Callus Culture

Plant tissue cultures are considered as potential producers of biologically active plant metabolites, which include various phenolic compounds that can be used to maintain human health. Moreover, in most cases, their accumulation is lower than in the original explants, which requires the search for factors and influences for the intensification of this process. In this case, it is very promising to use the precursors of their biosynthesis as potential "regulators" of the various metabolites' formation. The purpose of our research was to study the effect of L-phenylalanine (PhA, 3 mM), trans-cinnamic acid (CA, 1 mM) and naringenin (NG, 0.5 mM), as components of various stages of phenolic metabolism, on accumulation of various phenolic compound classes, including phenylpropanoids, flavans and proanthocyanidins, as well as the content of malondialdehyde in in vitro callus culture of the tea plant (Camellia sinensis L.). According to the data obtained, the precursors' influence did not lead to changes in the morphology and water content of the cultures. At the same time, an increase in the total content of phenolic compounds, as well as phenylpropanoids, flavans and proanthocyanidins, was noted in tea callus cultures. Effectiveness of precursor action depends on its characteristics and the exposure duration, and was more pronounced in the treatments with PhA. This compound can be considered as the most effective precursor regulating phenolic metabolism, contributing to a twofold increase in the total content of phenolic compounds, flavanes and proanthocyanidins, and a fourfold increase in phenylpropanoids in tea callus cultures.

Calcium- and Magnesium-Enriched Organic Fertilizer and Plant Growth-Promoting Rhizobacteria Affect Soil Nutrient Availability, Plant Nutrient Uptake, and Secondary Metabolite Production in Aloe vera (Aloe barbadensis Miller) Grown under Field Conditions

This work investigates the effects of an organic fertilizer enriched in Ca and Mg and two bacterial inoculants, applied alone and in combination, on soil fertility, plant growth, nutrition, and production of secondary metabolites, namely, acemannan and total phenolic compounds (TPCs), by Aloe vera (Aloe barbadensis Miller), under field cultivation. The first inoculum consisted of five native bacterial strains (Pseudomonas sp., Enterobacter sp., and three strains of Pantoea sp.), characterized in vitro as putative plant growth promoters, isolated from local organic farming fields of Aloe vera. The second inoculant was a commercial product (BACTILIS-S and HUMOFERT) and consisted of three Bacillus species: B. pumilus, B. amyloliquefaciens, and B. subtilis. The organic fertilizer (HUMO-CAL M-8O) was a mixture of humic and fulvic acids, with an additional CaCO3 (40% w/w) and MgO (4% w/w). The most significant increase in the content of acemannan and TPCs was detected under single application of the organic fertilizer, which was linked to enhanced concentration of Mg and Ca in the leaf gel. The concentration of acemannan tended to be increased with the combined application of the organic fertilizer and microbial inoculants. TPCs were significantly increased in both single and combined treatments, seemingly related to Fe concentration in the leaf rinds.

PLA films loaded with green tea and rosemary polyphenolic extracts as an active packaging for almond and beef

In the present study, RE presented higher antioxidant capacity and higher content of total phenolic compounds than GTE. While the main phenolic compounds identified in RE were carnosic acid, carnosol and rosmarinic acid, in GTE catechins, rutin and gallic acid were the main identifies compounds. Extracts were incorporated into PLA active films, followed by the evaluation of its properties. The potential of the active PLA films to extend foods shelf-life was tested in almonds and beef. PLA/4GTE presented the highest water vapor permeability and opacity, while PLA/4RE presented the highest O2 permeability. PLA/2 GTE and PLA/4GTE presented the highest total content in phenolic compounds at the end of 10 days (at 40 °C). Regarding the almond packaged with the active films, PLA with RE films were the most effective in the reduction of oxidation, allowing to obtain the lowest lipid oxidation products, at the end of 60 days of storage at room temperature. In addition, active PLA films inhibited the formation of MDA content in beef stored for 11 days. This study shows that these PLA active packages can contribute for delaying lipid oxidation in foodstuffs with high fat content.

Antioxidant Activities and Characterization of Polyphenols from Selected Northern Thai Rice Husks: Relation with Seed Attributes

Rice production generates a significant amount of agricultural waste. This study aimed to give results related to the existence of antioxidant phenols in agricultural waste of selected Northern Thai rice varieties. The antioxidant activities, contents of total flavonoids and phenolic compounds in the ethanolic rice husk extract were evaluated. The highest antioxidant activities were found in the variety PES1CMU, with 2,2ʹ-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid and 2,2-diphenyl-1-picrylhydrazyl as 679.66 and 4.16 mmol/(L·g) trolox equivalent, respectively, ferric reducing antioxidant power as 0.87 mmol/(L·g) Fe2+, total phenolic content as 29.90 mmol/(L·g) gallic acid and total flavonoid content as 12.16 mg/g catechin equivalent. Polyphenol compounds were identified mainly by standard polyphenols using the liquid chromatography mass spectrometry, with the highest contents of phytic acid, o-coumaric acid, naringin and kaempferol. The non-glutenous and wetland ecotypes of rice husk samples were the richest in antioxidant activities and polyphenol contents characterized by using principal component analysis. The glutenous rice husk contained higher antioxidant activities than the rest. Interestingly, quercetin is a significant phenolic compound that positively correlated with the overall antioxidant activities of rice husk. This finding will be relevant for future application of rice husk antioxidant components in the production of functional ingredients as well as for the food and pharmaceutical industries.

The Influence of Biopolymer Coating Based on Pumpkin Oil Cake Activated with Mentha piperita Essential Oil on the Quality and Shelf-Life of Grape

This work aimed to determine the influence of biopolymer coatings based on pumpkin oil cake, with and without the addition of Mentha piperita essential oil, on the quality and shelf-life of the Afus Ali variety of grapes, stored at room temperature and in the refrigerator. Furthermore, a 10% (w/w) aqueous solution of composite pumpkin oil cake (PuOC) with the addition of 30% glycerol was prepared at 60 °C and pH 10. The active biopolymer coating was prepared similarly by adding 1% (v/v) Mentha piperita essential oil. The quality of packed grapes was tested by determining the dry matter content, total sugar content, total acidity, alcohol content, total phenolic compounds content, and total flavonoid content, as well as by determining the antioxidant activity, through the application of the DPPH, FRAP and ABTS tests. Additionally, microbiological parameters were investigated: total aerobic microbial count, yeasts, and molds. The obtained results proved that in all tested samples, over a certain period of time, the content of dry matter, content of phenolic and flavonoids substances and sugar content decreased as a consequence of the spoilage of grapes, that is, the consumption of sugar for the production of alcohol, which consequently leads to the total acidity increasing. The application of lower storage temperatures and active coating (with Mentha piperita essential oil) had a positive effect on all inevitable reactions. Grapes’ antioxidant potential may be enhanced or maintained by applying PuOC coating with or without Mentha piperita essential oil, which is best observed in the case of the DPPH test. The uncoated sample stored at room temperature had the largest decrease in DPPH values during storage, with changes ranging from 2.119 mg/g to 1.471 μmol mg/g. The samples, coated with PuOC and PuOC with the addition of essential oil, had uniform DPPH values throughout the entire storage period. Additionally, regarding phenolic content, at the end of storage period the highest phenolic content was observed in samples with active coating stored at room temperature (734.746 ± 2.462) and at refrigerator temperature (680.827 ± 0.448) compared with untreated samples and with samples with plain PuOC coating. The presence of active essential oil in the applied coating significantly affected the microbiological profile of grapes during the storage period. Besides the positive impact of the applied lower storage temperature, the effectiveness of the applied active packaging is even greater (microbiological results were in the order of PuOC+essential oil < PuOC < Control). The developed artificial neural networks were found to be adequate for modeling the microbiological profile, antioxidant activity, phenolic and flavonoid content.

Arabic coffee infusion based kombucha: Characterization and biological activity during fermentation, and in vivo toxicity

In this study, arabic coffee infusion was used to produce a fermented beverage known as kombucha. The physicochemical, antioxidant and antimicrobial activities, as well as in vivo toxicity were evaluate throughout 21 days of fermentation. Reduction in pH and sugar levels were observed throughout the fermentation period. There was no significant difference in the content of total phenolic compounds between the unfermented and fermented beverage, nor between the fermentation times, as well as in the antioxidant activity. The 5-caffeoylquinic acid was identified at all fermentation times evaluated, and no significant difference was observed regarding its concentration. It showed antibacterial and antifungal activity against all strains tested. No toxic effect of the beverages was observed in the in vivo model (Galleria mellonella) studied. These results demonstrated that coffee infusion is a possible alternative for kombucha production since the physicochemical changes prove the metabolic activity of Symbiotic Culture of Bacteria and Yeast.

Schiff base crosslinked gelatin-Spirulina platensis protein concentrate films with enhanced antioxidant activity.

The aim of this work is to produce bioactive films suitable for aerobic packaging applications by combining the bioactivity of Spirulina platensisprotein concentrate (PC; 1% and 2% w/w), the sustainable nature of bovine gelatin (Ge), and sodium alginate dialdehyde (ADA, 5% w/w) as Schiff base crosslinking agent.PC was obtained by an optimized acid-base extraction process and characterized. PC showed a dose-dependent radical scavenging activity (RSA; IC50 =24.3mg/L) related to its high content of C-phycocyanin and total phenolic compounds (32.44±1.37mg gallic acid equivalents per gram of PC). As a general trend, crosslinking decreased the water solubility, improved mechanical properties, and helped improve RSA of Ge-ADA-PC films. Ge-5ADA-2PC film recorded best compromise between solubility (only 33.6%), high UV barrier (0.134% transmittance at 400nm), reasonable extensibility (217.00±2.34%), tensile strength (3.50±0.43MPa), water vapor permeability (2.00±0.17×10-12 kg·m/m2 ·Pa·s), and RSA (44.70±2.19%). Wrapping hake fillets in this filmdelayed lipid oxidation during storage under refrigerated conditions for 11days, maintaining the thiobarbituric acid indexbelow 0.5mg malonaldehyde/kg muscle. Results suggest that Ge-ADA-PC films have potential as aerobic packaging materials for oxidation-sensitive food. PRACTICAL APPLICATION: The combination of gelatin, alginate dialdehyde and Spirulina platensis protein concentrate gave rise to fully biobased films with reduced water solubility and enhanced antioxidant activity, which were able to delay the secondary lipid oxidation of refrigerated seafood. This study also shows the potential of cyanobacteria as renewable resources of high-value ingredients for the design of active and intelligent aerobic packaging solutions.

Evaluating the Quality of Raw Chocolate as an Alternative to Commercial Products

The aim of this presented study was the sensory evaluation, the analysis of the nutritional composition, and the analysis of the content of health-promoting ingredients, i.e., the total phenolic compounds and the total flavonoids, in raw chocolate (made exclusively from unroasted cocoa beans, cane sugar, and cocoa butter) in comparison to classic commercial chocolates available in the consumer market. The research showed that raw chocolate, compared to commercial chocolates, contained less carbohydrates (39.6% in raw chocolate and 42.7% and 53.7% in commercial chocolate, respectively), was characterized by a higher content of total phenolic compounds (22.08 mg GAE/g in raw chocolate and 9.25 and 7.87 mg GAE/g in commercial chocolates, respectively), had a a higher content of total flavonoids (8.75 mg ECE/g in raw chocolate and 4.43 mg/g and 3.83 mg ECE/g in commercial chocolates, respectively), and also had a higher total antioxidant activity (172.16 μM TE/g in raw chocolate and 132.60 and 63.76 μM TE/g in commercial chocolates, respectively). In the sensory assessment, raw chocolate received lower ranks than commercial chocolates, especially in the case of the most important discriminant in the entire evaluation, i.e., taste, which was unquestionably related to the lower content of carbohydrates. However, currently, it is necessary to reduce the consumption of sugar, which, when excessively present in the diet, is the cause of many diseases. However, in the sensory evaluation for the criteria “external appearance—shape” and “external appearance—surface condition,” the raw chocolate received the highest marks.

Study of Physico - Chemical and Nutritional Properties of Some Processed Pickles

This study was conducted in the laboratories of the Department of Food Science/College of Agriculture, University of Tikrit for the period 2016/10/1 to 2017/10/1. Results of chemical composition of processed pickles. They included(E:Sour cucumber pickle, F: Carrot pickle with spices herps (slow method),G:Cucumber pickles(rapid method),H: Carrot pickles(rapid method), I: Cucumber pickle with spices(slow method herps), J:Soure carrot pickle) showed significant increase to values of moisture ,dry matter ,protein ,fat, ash, carbohydrate, acidity and pH to the pickles J,F.I.G,E,F,H and H respectively, which they were 95.380%, 6.795% ,2.150%, 0.805%, 3.415%,1.910% ,3.130% and 3.99 respectively .Results of physical properties indicated that pickles samples of E,H were significantly higher than the other treatments at hardness and TSS which they were 15.00 mm and 13.00%, respectively .At storage, results revealed a decrease in values of acidity and hardness and increases at pH .The concentrations of Pb ,Cu, and Zn in local pickles were within permitted limits of international organizations or specifications ,WHO, USDA. Concentrations of Zn ,Fe, Ca, and Mg in processed pickles were at the ranges of (0.01-0.11),(0.15- 0.22),(0.09-0.15) and (0.01-0.17) M/ml .Results showed that the highest content of vitamins B1,B2,B6,B9,B3 and C were16.921,13.297 , 17.451,20.086 , 24.117,and12.597 M/ml for E,E,G,J,F, and H samples respectively .The total phenolic compounds content was within the range of 12.00-15.50mg/100g for each G,J respectively. While TPC content of fresh fruits L,K was 16.90 and 14.90 mg/100g respectively .The higher content of salicalic acid ,vanillin , catechol and recoresenol was revealed at H, E, E, and K samples ,its value was 17.0, 12.0, 8.4, 5.0 M/l respectively .
 

Study of Serial Exposures of an Astringent Green Tea Flavonoid Extract with Oral Cell-Based Models.

It is well known that repeated exposure to phenolic compounds (PCs) raises astringency perception. However, the link between this increase and the oral cavity's interactions with salivary proteins (SPs) and other oral constituents is unknown. To delve deeper into this connection, a flavonoid-rich green tea extract was tested in a series of exposures to two oral cell-based models using a tongue cell line (HSC3) and a buccal mucosa cell line (TR146). Serial exposures show cumulative PC binding to all oral models at all concentrations of the green tea extract; however, the contribution for the first and second exposures varies. The tongue mucosal pellicle (HSC3-Mu-SP) may contribute more to first-stage astringency (retaining 0.15 ± 0.01 mg mL-1 PCs at the first exposure), whereas the buccal mucosal pellicle (TR146-Mu-SP) retained significantly less (0.08 ± 0.02 mg mL-1). Additionally, increased salivary volume (SV+), which simulates the stimulation of salivary flow brought by a food stimulus, significantly enhances PC binding, particularly for TR146 cells: TR46-Mu-SP_SV+ bound significantly higher total PC concentration (0.17 ± 0.02 mg mL-1) than the model without increased salivary volume TR146-Mu-SP_SV- (0.09 ± 0.03 mg mL-1). This could be associated with a higher contribution of these oral cells for astringency perception during repeated exposures. Furthermore, PCs adsorbed in the first exposure to cell monolayer models (+TR146 and +HSC3) change the profile of PCs bound to these models in the second exposure. Regarding the structure binding activity, PCs with a total higher number of hydroxyl groups were more bound by the models containing SP. Regarding the SP, basic proline-rich proteins (bPRPs) may be involved in the increased perception of astringency upon repeated exposures. The extent of bPRP precipitation by PCs in mucosal pellicle models for both cell lines (HSC3 and TR146) in the second exposure (76 ± 13 and 83 ± 6%, respectively) was significantly higher than in the first one (25 ± 14 and 5 ± 6%, respectively).

Antimicrobial Properties of Essential Oils Obtained from Autochthonous Aromatic Plants.

The aim of this work was to determine the antimicrobial activity of the essential oils of six plants widely distributed in the Dehesa of Extremadura, such as Calendula officinalis, Cistus ladanifer, Cistus salviifolius, Cistus multiflorus, Lavandula stoechas, and Rosmarinus officinalis. The content of total phenolic compounds (TPC) and the antimicrobial activity of the essential oils against pathogenic and spoilage bacteria and yeasts as well as aflatoxin-producing molds were determined. A great variability was observed in the composition of the essential oils obtained from the six aromatic plants. The Cistus ladanifer essential oil had the highest content of total phenols (287.32 ppm), followed by the Cistus salviifolius essential oil; and the Rosmarinus officinalis essential oil showed the lowest amount of these compounds. The essential oils showed inhibitory effects on the tested bacteria and also yeasts, showing a maximum inhibition diameter of 11.50 mm for Salmonella choleraesuis and Kregervanrija fluxuum in the case of Cistus ladanifer and a maximum diameter of 9 mm for Bacillus cereus and 9.50 mm for Priceomyces carsonii in the case of Cistus salviifolius. The results stated that antibacterial and antiyeast activity is influenced by the concentration and the plant material used for essential oil preparation. In molds, aflatoxin production was inhibited by all the essential oils, especially the essential oils of Cistus ladanifer and Cistus salviifolius. Therefore, it can be concluded that the essential oils of native plants have significant antimicrobial properties against pathogenic and spoilage microorganisms, so they could be studied for their use in the industry as they are cheap, available, and non-toxic plants that favor the sustainability of the environment of the Dehesa of Extremeña.

Subterranean Clover and Sulla as Valuable and Complementary Sources of Bioactive Compounds for Rainfed Mediterranean Farming Systems.

Mediterranean pasture and forage legumes are important components of sustainable production systems. Subterranean clover and sulla represent key species having proven high agronomic value and traits for production and multiple services. Our research investigated the potential of the abovementioned species as a source of phenolic compounds and antioxidants for contributing to support their full exploitation in the fodder, animal welfare, and nutraceutical sectors. Antioxidant capacity, as well as the content of total phenolic compounds and individual phenolic compounds, was determined in subterranean clover and sulla shoots at the vegetative, flower bud, flowering, and seed ripening phenological stages. The antioxidant capacity and the phenolic content were affected significantly by harvest time. In subterranean clover, 10 individual phenolic compounds were detected, and isoflavones were the most abundant (3.19-18.27 mg·g-1 DM). Eleven phenolic compounds were identified in sulla shoots, and chlorogenic acid (0.76-3.43 mg·g-1 DM) and diosmin (3.64-4.94 mg·g-1 DM) were the most represented compounds at the vegetative and flower bud stage. On the basis of our findings, a complementary utilization of both legume species is suggested; this might ensure the exploitation of all phenolic compounds in view of the potential benefits for animal production and health.

Developmental, Phytochemical and Enzymatic Changes in Pot Marigold (Calendula officinalis L.) cvs. Hybrid and French with Salicylic Acid (SA) and Polyamine Spermidine (SP) Foliar Spray

Marigolds (Calendula officinalis L.) are valuable in ornamentation, human food, and other uses; to enhance productivity, plant growth regulators produce stimulatory effects, including salicylic acid (SA) and spermidine (SP), but there is a lack of scientific evidence about such effects in marigolds. The study assessed, under greenhouse conditions, changes in physico-chemical parameters, enzymatic activity, and bioactive compounds of marigold cvs. Hybrid and French marigolds were sprayed of SA (1 and 2 mM) and SP (2 and 3 mM) and compared to control (pure water). The SA at 2 mM improved leaf length (8.20 cm), flower height and diameter (5.32, 8.28 cm), flower fresh and dry weight (14.30, 1.5 g), and the maximum number of flower petals (55) in ‘Hybrid’. Similarly, 2 mM SA gave the maximum number of leaves (40.71) and stem thickness (5.76 mm) in ‘French’, but 3 mM SP promoted the maximum plant height in ‘Hybrid’. Superoxide dismutase, peroxidase, and catalase activities increased in ‘Hybrid’ with 2 mM SA; with this SA dose, ‘Hybrid’ had higher contents of total phenolic compounds (68.34 mg GAE g−1), antioxidants (77%), carotenoids (110 mg 100 g−1), and flavonoids (67.5 mg RE g−1) than the control. The best dose for improving growth in both marigold varieties was 2 mM SA.

Hedypnois cretica L. and Urospermum picroides L. Plant Growth, Nutrient Status and Quality Characteristics under Salinity Stress

The cultivation of tolerant species with low-quality irrigation water is one of the main strategies to address the lack of availability of irrigation water. In this work, the effect of salinity on plant growth, nutritional composition, and quality features of Hedypnois cretica and Urospermum picroides was tested. Fresh yield of leaves and roots of both species were severely decreased under high salinity, while the mineral profile of leaves and roots also showed a decrease in most minerals. The recorded values of K/Na and Ca/Na ratios indicate that H. cretica has a higher susceptibility to salt stress due to a higher decrease in the values of the respective ratios compared to U. picroides. Leaf pigments and total phenolic compounds content were not significantly affected by salinity, while total soluble solids and titratable acidity increased under increasing salinity in most cases. Moreover, free proline content increased with increasing salinity, while the opposite trend was recorded for nitrates’ content. In conclusion, our findings indicate that tailor-made nutrient solutions could allow the use of irrigation water of a low quality for the commercial cultivation of H. cretica and U. picroides, thus allowing their domestication and integration in cropping systems where the cultivation of conventional crops is compromised.