Total Coliform Concentrations Research Articles

Salinity and microbiological risk assessment in surface runoff and main tributaries to La Vega Dam in Teuchitlán, Jalisco, Mexico.

Objective: This research aimed to evaluate the water quality of the main tributaries to the La Vega Dam for irrigation purposes through the physicochemical and microbiological parameters. Design/methodology/approach: The 10 main tributaries to the La Vega Dam were selected. The variables evaluated were pH, Temperature, Electrical Conductivity, Total Dissolved Solids and Dissolved Oxygen, boron calcium, magnesium, sodium, SAR, and RCS. Results: 50% of samples presented low sodicity risk, 10% medium risk, 20% and 20% very high risk. In the CSR results, only one site (10%) presented a low risk, and the remaining four tributaries (40%) of this area presented a medium risk. Five sites (50%) located east of the dam presented a high risk. Also, high boron concentration was observed, corresponding to El Salado River. Fecal pollution was detected in 100% of sites, limiting their use as irrigation water. Limitations on study/implications: Continuous water quality monitoring of the main tributaries to and into La Vega Dam should be followed up. Findings/conclusions: The most significant contribution of contamination to the dam was observed in the eastern part, with the entrance of El Salado River. High concentrations of boron were also observed. A high concentration of total and fecal coliforms was also found, in addition to the presence of Pseudomonas spp., which indicates fecal contamination. The effluent at the east of La Vega Dam is unsuitable for irrigation.

The Potential of Spring Water Analysis for Drinking Water at Ujung Gagak Village, Kampung Laut Subdistrict, Cilacap Regency

Limited clean water is the main problem in Ujung Gagak Village. The community can use no groundwater because the water is brackish and salty, which is unsuitable for daily activities. This study aims to analyze the potential of the Karang Cave spring to be used as drinking water. This spring plays an important role in the life of the people of Ujung Gagak Village when the dry season arrives. Using the grab sampling method, raw water sampling at the Karang Cave spring. Pollution Status Index (IP) quality of 5.08 is included in the moderately polluted category. Then, raw water samples are taken and processed in the Reverse Osmosis (RO) system. Parameters that exceeded the quality standard were total coliform of > 24,000 CFU/100ml, salinity parameter of 0.33‰, turbidity of 1.98 NTU, and hardness of 320 mg/L. However, treating it in an RO system for drinking water is necessary. Laboratory test results for water quality after processing, several parameters experienced a significant decrease, for the parameter salinity to 0‰, turbidity to 0.74 NTU, and hardness to 99 mg/L, total coliform decreased by 108 CFU /100ml. The results showed that the RO system succeeded in removing salt up to 99.5% and reducing the Concentration of turbidity, hardness, and total coliform. It is suitable for drinking water, but if you want to drink it immediately, you can cook it first, considering that the total coliform is still above the required quality standard.

Effect of water treatment processes on microbial contamination in drinking water in rural areas of the urban periphery

ABSTRACT The water treatment infrastructure facilities play an important role in ensuring the safety of drinking water. A survey of the drinking water treatment process was conducted in the urban peripheral areas of Beijing, China, and the main water treatment infrastructure facilities in terms of their impact on microbial contamination were investigated. The sedimentation equipment, filtration facilities, and disinfection equipment are all significantly correlated with the concentration of heterotrophic plate counts in drinking water. The filtration facilities and disinfection equipment were also positively correlated with the concentration of total coliform and Escherichia coli. The effect of removing microorganisms by different water disinfection methods gradually decreases in the order of ozone, chlorine, chlorine dioxide, and ultraviolet light. The effect of microbial contamination removal of different water pumping methods is as follows: direct water supply > pressure tank > secondary pressing pump station > water tower > high-level water tank, and the removal effects are 7.6, 7.4, 4.1, 3.6, and 1.7 times that of self-flowing water supply. The study provides scientific support for the renovation, and upgrading of microbial pollution reduction in drinking water in rural areas of the urban periphery.

Enhancement of solar water disinfection using different types of clay

This study delves into the impact of incorporating natural nanoparticles (mineral clays) into contaminated water during the Solar water disinfection (SODIS) process. The experiment involved introducing contaminated water samples into glass containers containing 1 × 10−3 gm/ml of a specific clay type. These containers were then exposed to solar radiation for varying time intervals. Subsequently, samples were extracted from each container, and the total counts of Total Coliform, P. aeruginosa aeruginosa, and E.coli were measured using the IDEXX setup. The results revealed that Zeolite, a specific type of clay, exhibited the shortest disinfection process among the mineral clay samples. The optimal concentration of Zeolite, which effectively reduced the concentration of Total Coliform, P. aeruginosa, and E.coli concentration, was 1 × 10−3 gm/ml. In conclusion, adding Zeolite to wastewater was observed to positively impact the disinfection process, accelerating the inactivation process by reducing the number of pathogenic microorganisms.

Conditions of total coliform in the Aur River, Palembang City, South Sumatra, Indonesia

Pathogenic microorganisms present in river water pollution are a serious health risk. Escherichia coli is a pathogen commonly found in water. Escherichia coli is an indicator of water contaminated with feces. These pathogens have an impact on human health. Aur River is one of the tidal rivers in Palembang City. From the observations, many people dispose of domestic waste into river bodies, so the river is at risk of being polluted by pathogenic microorganisms. When it recedes, the water looks pitch black, and when it is high, many people bathe, brush their teeth, and wash their clothes in this river. Thus, this study aimed to monitor water quality, including total coliform, of the Aur River Palembang City. The results of this monitoring can be used as the basis for making a policy and strategy to control the quality of this river water. This study was carried out on the Aur River, which is 2.5 km long. Measurements were made at three stations, namely at the upstream, middle, and downstream. Based on measurements of the pH, BOD, COD, DO, and total coliform concentrations, it is concluded that the Aur River has been polluted because the BOD, COD, DO, and total coliform were above the quality standards. The pH is still at the quality standard.

Monitoring microbial safety and evaluation of removal efficiency from drinking water in Ahvaz, Iran

The availability of potable water sources is vital worldwide. Due to the potential for microbial contamination, developing nations focus more research on the existence of microorganisms that are indicators in water than they do on chemical presence. This study was done in order to assess the microbial level of drinking water in Ahvaz city. In this descriptive-cross-sectional study, the measure the microbial tests, two culture mediums, MacConkey broth, and green lactose broth, were used for possible and confirmatory culture respectively. Bioindicators measured in drinking water included fecal coliform and total coliform. Not only the residual chlorine concentration was also measured, but its relationship with bioindicators was also calculated by Spearman's correlation test. Result of this study showed that, the maximum total coliform concentration reported is between 2021 and 2022 (1600 MPN/100 ml). Based on result of this study the average concentration of fecal coliform in 2019, 2020, 2021, and 2022 were 11.88, 10.66, 7.78, and 6.11 MPN/100 ml respectively. The average annual concentration of total coliform and fecal coliform in four years was 28.95 and 9.32 MPN/100 ml, respectively. According result of this study, the Karun River, Ahvaz's main drinking water source, is contaminated by surface water runoff, old facilities, and unfiltered discharge of different wastewater kinds from factories, farms, and communities in Khuzestan.

Occurrence and Mitigation of Bacterial Regrowth in Stored Household Water in Eastern Coastal Madagascar

In communities where people lack on-demand, safely managed drinking water, stored household water often becomes contaminated by fecal bacteria, regardless of the source-water quality. The objectives of this paper are to assess and control bacterial contamination in stored household water in Toamasina, a rapidly urbanizing city in eastern coastal Madagascar. We collected samples of source water and stored household water from 10 representative households that use different water sources and different storage strategies, and we analyzed the samples for several fecal indicator bacteria. We also tested three methods that residents of Toamasina could realistically employ for cleaning their household water storage vessels, assessing the effect of the cleaning methods on measured bacterial levels in the water. Consistent with the previous literature, we found that concentrations of total coliforms in stored household water were significantly higher than in the corresponding source water (p < 0.05). In 100% of households that stored their water in 20 L polyethylene jerrycans (n = 4), biofilms on the walls of the jerrycan harbored total coliforms and Enterococcus. The use of a closed storage container was, on its own, not found to provide a meaningful protective effect against bacterial regrowth; to be protective, closed storage containers must be combined with high-quality source water and/or with adequate cleaning to prevent biofilm formation. A dilute solution of sodium hypochlorite, known locally as Sûr’Eau or Manadio Rano, was both the most effective and the least expensive method for cleaning household water storage containers. We conclude that regular and effective cleaning of storage containers is an essential component of safe water storage. Because household storage of collected water is common in many low- and middle-income countries, these results are important towards the worldwide achievement of the United Nations’ Sustainable Development Goal 6.

Potensi Pemanfaatan Enzim Biokatalitik dari Kulit Buah dan Sayur untuk Pengolahan Limbah Cair Rumah Sakit

Health services in hospitals produce hazardous and infectious waste that comes from sharing sources of activity. The research carried out experimentally in laboratory aims to determine the effect of biocatalytic enzyme treatment from fruit and vegetable peel (concentration variations of 5%, 10%, 15%) on pH, chemical oxygen demand (COD) levels, total suspended solids (TSS), and total coliforms of hospital liquid waste. From the research results, it was found that the addition of biocatalytic enzymes was very effective in reducing the total coliform concentration. The highest reduction in coliforms occurred with the addition of 15% enzyme, where there was a decrease from 1600 MPN/100 mL to 500 MPN/100 mL at a digestion time of less than 24 hours. After a digestion time of 2 days, no coliforms were found in the wastewater samples. However, there was a decrease in pH and an increase in TSS and COD concentrations which did not meet quality standards after being treated with enzymes at all concentration variations. The profile of changes in TSS and COD concentrations shows a decreasing trend with an increase in digestion time of 1 to 3 days, the values ​​obtained will still exceed the TSS and COD quality standards (30 mg/L and 100 mg/L). Meanwhile, the waste pH gradually increased with increasing digestion time up to 3 days at enzyme concentrations of 5% and 10%, namely 3.5 and 4.8. However, this value is still outside the permitted quality standard interval, namely 6-9.

Occurrence and concentrations of traditional and emerging contaminants in onsite wastewater systems and water supply wells in eastern North Carolina, USA.

Onsite wastewater treatment systems (OWTSs) and private wells are commonly used in Eastern North Carolina, USA. Water from private wells is not required to be tested after the initial startup, and thus persons using these wells may experience negative health outcomes if their water is contaminated with waste-related pollutants including bacteria, nitrate or synthetic chemicals such as hexafluoropropylne oxide dimer acid and its ammonium salt (GenX). Water samples from 18 sites with OWTSs and groundwater wells were collected for nitrate, Escherichia coli (E. coli), total coliform, and GenX concentration analyses. Results showed that none of the 18 water supplies were positive for E. coli, nitrate concentrations were all below the maximum contaminant level of 10 mg L-1, and one well had 1 MPN 100 mL-1 of total coliform. However, GenX was detected in wastewater collected from all 18 septic tanks and 22% of the water supplies tested had concentrations that exceeded the health advisory levels for GenX. Water supplies with low concentrations of traditionally tested for pollutants (nitrate, E. coli) may still pose health risks due to elevated concentrations of emerging contaminants like GenX and thus more comprehensive and routine water testing is suggested for this and similar persistent compounds.

Logistic Model for Predicting Coliform Growth in Wastewater Effluent From the Chambo River (Ecuador)

Purpose: The objective of this work is to apply a predictive model of bacterial growth to determine the concentration of total coliforms, under controlled laboratory conditions, in wastewater from the city of Riobamba that flows into the Chambo River (Ecuador). Theoretical Framework: Wastewater pollution is a global problem that affects water quality and public health. Population growth has a drastic impact on the contamination of water bodies, particularly in Ecuador, since most of the effluents are not adequately treated, which becomes a public health and environmental problem. For this reason, over the years, total and fecal coliforms have been used as indicators of water quality. Design/Methodology/Approach: The study used a mathematical modeling approach of a logistic and deterministic type, and calculated through an executable command designed in the integrated development environment (IDE) with the Visual C# programming language (logic) and the XAML design language (interface). Findings: The results achieved are 1) the theoretical construction of a mathematical model (logistic model) starting from a differential equation and the taking of three experimental measurements, 2) the computer construction of the bacterial growth model and 3) it has been demonstrated that there is agreement between the values obtained from the model of total coliform growth and the experimental results with a margin of error of less than 1%. Research, Practical & Social Implications: This information can be used by stakeholders to determine actions that could improve current conditions. Originality/Value: The result of this study emphasizes that the model proposed can reproduce the growth of coliforms up to a maximum growth point, it is necessary to continue with the development of the same in the stationary phase of bacterial growth and then in the decay phase.

Logistic Model for Predicting Coliform Growth in Wastewater Effluent From the Chambo River (Ecuador)

Purpose: The objective of this work is to apply a predictive model of bacterial growth to determine the concentration of total coliforms, under controlled laboratory conditions, in wastewater from the city of Riobamba that flows into the Chambo River (Ecuador). Theoretical Framework: Wastewater pollution is a global problem that affects water quality and public health. Population growth has a drastic impact on the contamination of water bodies, particularly in Ecuador, since most of the effluents are not adequately treated, which becomes a public health and environmental problem. For this reason, over the years, total and fecal coliforms have been used as indicators of water quality. Design/Methodology/Approach: The study used a mathematical modeling approach of a logistic and deterministic type, and calculated through an executable command designed in the integrated development environment (IDE) with the Visual C# programming language (logic) and the XAML design language (interface). Findings: The results achieved are 1) the theoretical construction of a mathematical model (logistic model) starting from a differential equation and the taking of three experimental measurements, 2) the computer construction of the bacterial growth model and 3) it has been demonstrated that there is agreement between the values obtained from the model of total coliform growth and the experimental results with a margin of error of less than 1%. Research, Practical & Social Implications: This information can be used by stakeholders to determine actions that could improve current conditions. Originality/Value: The result of this study emphasizes that the model proposed can reproduce the growth of coliforms up to a maximum growth point, it is necessary to continue with the development of the same in the stationary phase of bacterial growth and then in the decay phase.

Effect of dissolved ozone flotation thickening process on coliform bacteria and antibiotics simultaneous abatement: A pilot-scale study

This study focused on the removal of the total coliforms, fecal coliforms and four target antibiotics in the dissolved ozone flotation (DOF) thickening sludge process. Additionally, the thickened effluent chromaticity and its effect on thickened sludge hydrolysis process were investigated. Ozonation in the DOF process could inactivate coliforms by oxidizing cellular components and destroying genetic material, as well as altering the chemical structure of antibiotics, leading to the degradation of antibiotics. At an O3 dosage of 16 mg/g TS, the concentration of total coliforms and fecal coliforms decreased by 2.2 log and 2.4 log, corresponding to an overall removal rate of 99.4 % and 99.7 %, respectively. The total degradation rate of four target antibiotics (tetracycline (TC), oxytetracycline (OTC), norfloxacin (NOR), ofloxacin (OFL)) were 66.5 %, 68.8 %, 53.3 % and 57.5 %, respectively. The chromaticity removal rate of the thickened effluent reached 95 %. Analysis of fluorescence spectra indicated alterations in the fluorescence properties of dissolved organic matter, resulting in a decrease in fluorescence intensity by ozonation. The thickened sludge had higher hydrolysis rates, resulting in a greater production of volatile fatty acids (VFAs). This was mainly attributed to the increased amount of soluble protein and carbohydrate in the substrate after DOF treatment, which was more conducive for the rapid conversion of hydrolysis into VFAs during the initial stage. These results provided new ideas for upgrading and transforming the thickening process of wastewater treatment plants (WWTPs).

Effectiveness of communal wastewater treatment plant in peri-urban Yogyakarta, Indonesia, for Escherichia coli removal

Yogyakarta Special Region, one of Indonesia’s provinces, has many communal wastewater treatment plants (CWWTPs). However, less than 40% of the province’s CWWTPs are operationally viable. Based on data from the Environmental Agency of Sleman Regency, the physical and chemical parameters of approximately 163 CWWTPs were evaluated. Their biological characteristics, however, still need to be understood. This study aimed to investigate the efficacy of CWWTPs in terms of biological parameters, specifically total Coliform and Escherichia coli (E. coli) concentrations in the observed CWWTPs. Five CWWTPs were used as study sites, namely IPAL Manunggal Pringgodani Sejati, IPAL Bakti Warga, IPAL Tambakrejo Bersih, IPAL Nologaten Bersih, and IPAL Andum Roso, which were classified as extremely high and high sanitation risk areas. The total Coliform and E. coli concentrations were determined using MPN techniques based on APHA 9221 (2017). The study found that the E. coli removal effectiveness of the CWWTPs in the very-high sanitation risk areas, namely IPAL Manunggal Pringgodani Sejati, IPAL Bakti Warga, and IPAL Tambakrejo Bersih, was 49%, 18%, and 83%, respectively. Furthermore, in high sanitation risk regions, such as IPAL Nologaten Bersih and IPAL Andum Roso, the CWWTPS were 17% and 73%, respectively. Total Coliform concentrations at all CWWTPs were below the standard limit set by Permen LHK No 68 and the EPA. The findings of this study will help to improve understanding of the WWTP process for E. coli elimination, which will, in turn, help to improve CWWTP performance.

Faucet-mounted point-of-use drinking water filters to improve water quality in households served by private wells

Approximately 13 % of Americans rely on private wells for household potable water. As private wells are not regulated beyond initial construction and often employ limited or no treatment, source water from wells can be vulnerable to contamination. While several studies have assessed applications of point-of-use (POU) filters in improving municipal tap water quality, few have investigated their use with private well water. This effort aims to build on previous examinations of POU treatment as a strategy to reduce adverse household drinking water exposures by: 1) assessing the effectiveness of commercially available faucet-mounted POU filters for improving microbial and chemical water quality in homes with private wells; and 2) documenting household ease of use and satisfaction with the filters. Faucet-mounted POU filters were distributed to 21 homes reliant on private wells in southern West Virginia and southwestern Virginia. Study participants were asked to collect water samples from two taps in their homes pre-filter installation, and again two-weeks and four-weeks post-installation. Participants filled out surveys about perceptions of their drinking water and the filter. Concentrations of Total Coliform, Ba, Cd, Cr, U, Cu, Pb, Al, Fe, Mn, Zn, and Sr were significantly lower (p < 0.05, Wilcoxon Rank Sum) in filtered water samples compared to paired unfiltered samples (n = 42) for the study period. However, concentrations of certain contaminants in filtered samples from homes with high levels of source water contamination still exceeded drinking water standards. Less than half of study participants reported that they intended to keep using the filters, citing issues of flowrate. Our findings suggest that faucet-mounted POU filters, while effective in reducing contaminants, might not be an appropriate intervention to improve water quality for all homes on private well water. Future investigation is required to improve filter user satisfaction and better assess appropriate source water chemistries for implementation.

Removal of Pathogenic Bacteria in a Horizontally Fed Subsurface Constructed Wetland Hybrid System

The management of effluents and their treatment is a fundamental issue in water management, the removal of different types of contaminants is another relevant issue for public health and the environment. Bacteria are one of the main types of contaminants in untreated water discharged to receiving bodies. The objective of this research was to evaluate the removal efficiency of pathogenic bacteria in a horizontal feeding subsurface artificial wetland that treats wastewater originated from the Boca de Río Technological Institute, Veracruz, Mexico. A hybrid system composed of seven cells with three types of substrates and ornamental type vegetation was designed; the indicators evaluated were the concentration of total and fecal coliforms and the efficiency of bacterial removal in the stages of the system. The artificial wetland system demonstrated a significant reduction (p &lt; 0.05) between the different cells of the system. The values of pathogenic bacteria removal obtained in the wetland were higher than 99% in the cells of the system and times. In conclusion, it was identified that the interaction of the components of this system and its operation under the climatic seasons of the site influenced the removal efficiency of pathogenic bacteria, allowing optimal removal efficiency.

Assessment of solid and liquid wastes management and health impacts along the failed sewerage systems in capital cities of African countries: case of Abidjan, Côte d'Ivoire

The management of domestic wastewater and rainwater is a major concern for the population of Yopougon. The study presents the causes of wastewater discharge from dysfunctional sewers and their health impacts on the population. It also highlights the environmental and health risk associated with poor solid and liquid waste management. This was based on literature search, semi-participatory workshop, physicochemical and bacteriological characterization of wastewater and finally through a household survey. The field survey was conducted on 245 household heads obtained using the Canadian statistical guidelines. The results obtained indicated that all main pollution indicators were; total nitrogen (TN, 525 ± 0.02 to 3077 ± 0.3 mg/l), nitrates (NO3, 146 ± 0.01 to 1347 ± 0.12 mg/l), biochemical oxygen demand (BOD, 278 ± 195.16 to 645 ± 391.74 mg/l), chemical oxygen demand (COD, 940 ± 650.54 to 4050.5 ± 71.42 mg/l) and total dissolved solids (TDS, 151 ± 9.9 to 766 ± 237.59 mg/l) which were above the values recommended by the World Health Organization (WHO) and Cote d'Ivoire national policy guidelines standards for the discharge of effluents into the environment. The analysis of the bacterial flora of the effluents revealed that the concentrations of Total Coliforms and fecal streptococci exceeded the values recommended by the WHO and national policy guidelines standards. This means that the populations of this area are prone to infectious diseases. Diseases such as malaria (84.53%), respiratory infections (61%), diarrhea (48.66%), intestinal diseases (44.5%), and typhoid fever (28.84%) were prevalent in the surveyed households.

Growth Performance of Mytilus galloprovincialis Lamarck, 1819 under an Innovative Integrated Multi-Trophic Aquaculture System (IMTA) in the Mar Grande of Taranto (Mediterranean Sea, Italy)

The cultivation of the Mediterranean mussel, Mytilus galloprovincialis Lamarck, 1819, has been tested in an innovative Integrated Multitrophic Aquaculture system (IMTA) in the Mar Grande of Taranto, as part of the EU-funded Remedialife project. This farming method could solve several problems including the low growth rate in mesotrophic environments while reducing the environmental impact of fish mariculture. Three productive cycles have been carried out. The first (2018–2019, traditional experiment) was conducted in three long lines around six cages of the fish farm in order to evaluate total mussel production under the innovative IMTA system and quality for human consumption by analyzing the concentration of culturable heterotrophic bacteria, total and fecal coliforms, Escherichia coli and Salmonella spp. in mussel tissues. In addition, 17 polycyclic aromatic hydrocarbons (PAHs), including 16 EPA priority compounds and seven polychlorinated biphenyls (PCBs), which are indicators of PCB contamination in the environment, were analyzed using gas chromatography in conjunction with a mass spectrometer. The second cycle (2020–2021, horizontal distance experiment) aimed to test the influence of fish cages on mussel growth by placing mussels near and far from the fish cages. The third cycle (2021–2022, vertical distance experiment) aimed to overcome the phenomenon of “heat waves” that can occur in the Mar Grande of Taranto during summer by testing the growth performance of mussels at two different depths (1 and 12 m). The following parameters were measured: Shell Length, L (mm); Shell Dry Weight, SDW (g); Flesh Dry Weight, FDW (g); Condition Index, IC = FDW/SDW. The results showed that the best growth performance was obtained near the fish cages and at a depth of 12 m. Moreover, the indicators of microbial contamination and concentrations of chemical compounds analyzed in mussel tissues cultured under the innovative IMTA system were in compliance with the reference values of European regulations.

Hydrogeochemical characteristics of Kodku River of Lalitpur District, Nepal

Abstract Hydro-geochemical characteristics of Kodku River of Lalitpur, Nepal were determined through analysis of physicochemical parameters including major anions and cations. This study also characterizes and evaluates the suitability for drinking and irrigation. The results showed that river water is slightly alkaline toward the downstream. The concentration of BOD, COD, and total coliform was much higher than its prescribed limits for drinking purposes in downstream, whereas a few samples in upstream are fit for drinking with reference to WQI value. The estimated sodium percentage (Na%), sodium adsorption ratio (SAR), permeability index (PI), magnesium adsorption ratio (MAR), and Kelly's ratio (KR) depict the excellent and safe categories for irrigation purposes. The obtained Wilcox diagram also revealed that river water falls under the C1S1 category which is safe for crops. Hydrogeochemical facies in Piper diagrams signifies the dominance of Ca–HCO3 type. Similarly, Gibbs maps indicate mineral (rock) weathering, whereas mixing diagram reveals carbonate weathering as the main controlling factor of major ion species. This study provides information on the hydrogeochemical properties of the river and its suitability for drinking and irrigation. Despite the comprehensiveness, the study can be an effective approach for countries with similar levels of water resources management.

Analysis of River Water Quality Based on Pollution Index Water Quality Status, Lombok District, NTB

Leneng River is one of the rivers in Praya City, Central Lombok Regency, which receives domestic, agricultural, and household industrial waste. Various community activities along the banks of the Leneng River have affected river water quality. Some communities on the banks of the Leneng River, from upstream to downstream, still use river water for their daily needs. This study aims to 1) analyze the water quality of the Leneng River in terms of environmental parameters (Physical, chemical, and biological) and 2) Determine the level of pollution of the Leneng River water using the Pollution Index (IP) method. River water quality measurements were carried out at 3 (three) sample points. Parameters measured and observed were physical parameters, namely Total Solid Suspend (TSS); chemical parameters, namely pH, Total Phosphate (T.PO4), Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Dissolved Oxygen (DO), and biological parameters namely Total coliform and Fecal Coliform. This type of research is survey research with a quantitative descriptive approach. The results showed that the TSS value at sample point 1 (upstream) exceeded the required quality standard; the highest TSS concentration value was 54 mg/L, and the lowest at sample point 2 (middle section) was 2.50 mg/L. Value parameter concentration pH 7.7-8.7, T.PO4 value 0.05 mg/L-0.29 mg/L, BOD value 0.17-0.42 mg/L, COD concentration value 8 mg/L -9mg/L, and DO value of 6.07 mg/L-7.57 mg/L, this shows that the concentration for chemical parameters still meets the class I water quality standards according to PP 22 of 2021, while the total coliform parameter value and Facel coliform have exceeded class I water quality standards. The total coliform concentration value is 10,000 - 20,000 MPN/100 mL, and the Fecal coliform value is 600 MPN/100 mL – 2,500 MPN/100 mL, while based on the Pollution Index (IP) quality status water in the upstream with a value of 2.84, the middle part of 1.97, and the downstream part of 2.84, this shows a decrease in water quality from upstream to downstream of the river, with a level of pollution in the lightly polluted category.

Effectiveness of the tropical plants Rhynchospora corymbosa and Coix lacryma-jobi in vertical flow constructed wetlands for municipal primary sewage effluent treatment

The performance of two tropical plants, Rhynchospora corymbosa L. (RC) and Coix lacryma-jobi, L (CL) in treatment of primary sewage effluent in lab-scale vertical-flow constructed wetlands (VFCW) along with no plant control wetland was investigated. A batch-flow VFCWs were operated under batch fill and drain hydraulic loading system with hydraulic retention times (HRT) of 0.5, 1, and 2 days and fill rate of 8 L/day. Removal of solids, organics, nutrients, and pathogens were monitored. The volumetric contaminant removal rates were best described by 1st order kinetics except for ammonia and phosphate, which was best described by Stover-Kincannon kinetics. Influent TSS, PO4 3−, COD, BOD5, and total coliform concentration were low but high in NH4 + concentration. CL was better in nutrient removal as HRT increases compared to RC. RC was more efficient at TSS, turbidity, and organics removal. Pathogen removal was independent of plant type but HRT. Solids and organic removal were lower in CL planted CWs due to preferential flow paths created by their bulky root. CL planted CWs removed more nutrients followed by RC planted CWs and then no-plant control CWs. The results of these tests demonstrate that both CL and RC are suitable for the treatment of municipal wastewater in VFCW system.