Total Chlorophyll Content Research Articles

TiO2 nanoparticles: Green synthesis and their role in lessening the damage of Colletotrichum graminicola in sorghum.

Fungal pathogens pose a persistent threat to crop plants, risking global food security. Anthracnose of sorghum caused by Colletotrichum graminicola causes a considerable loss in sorghum production. This study aimed to manage the anthracnose disease in sorghum using green-synthesized TiO2 nanoparticles using pomegranate peel and to assess their impacts on the agroeconomic attributes of sorghum. Synthesized TiO2 nanoparticles showed strong dose-dependent antifungal activity against C. graminicola and significantly reduced mycelial radial growth, comparable to commercial fungicides. Foliar application of TiO2 at concentrations of 150 and 200 ppm reduced the disease index >60% in pot trials. Additionally, the effect of TiO2 NPs on the growth and yield of sorghum plants and the possible mechanism(s) behind the suppression of anthracnose disease were deciphered. TiO2 NPs also improved shoot and root length, biomass accumulation, penile size, number of grains, and grain weight in sorghum plants infected with C. graminicola. Application of TiO2 NPs significantly increased the content of defense-related biochemicals, including total phenolic contents, activities of defense-related enzymes (PO, PPO, and PAL), photosynthetic pigments (total chlorophyll contents and carotenoids), and total protein contents. Collectively, our study verified the potential of green-synthesized titanium dioxide nanoparticles to suppress anthracnose disease by activating a defense system and stimulating growth and yield promotion under pathogen stress.

Effect of TopDressing on Chlorophyll Content in Barley Leaves and Its Relation to Grain Quality Parameters

The effect of top-dressing at tillage phase by Polydon® Amino Start organomineral fertilizer and KAS-28 liquid mineral fertilizer on the state of the leaf pigment complex of spring barley cv. Novichok, Rodnik Prikamya and Pamyati Rodinoy and its relationship with grain quality parameters was studied under field conditions of the Kirov region of the Russian Federation in 2020–2022. Significant effect of top-dress treatments on pigment content in the flag and second leaves was revealed. The use of top-dressing with KAS-28 enhanced the association of grain quality parameters with the chlorophyll pigment content of the two upper leaves compared to the control variant. The effect of Amino Start was not uniform: some of the connections were somewhat weakened compared to the control, some intensified, and some became statistically significant. The total content of chlorophyll correlated with the content of cellulose and fat in the grain in all treatments. Compared to controls, the use of top-dress treatments altered the effectiveness of using of photoassimilates of both leaves for cellulose and fat synthesis. The obtained data indicate the possibility of predicting the content of the main parameters of spring barley’s grain quality by the content of chlorophyll pigments in flag or second leaves of plants during the flowering phase.

Engineered Chlorella vulgaris improves bioethanol production and promises prebiotic application.

Microalgal biomass for biofuel production, integration into functional food, and feed supplementation has generated substantial interest worldwide due to its high growth rate, non-competitiveness for agronomic land, ease of cultivation in containments, and presence of several bioactive molecules. In this study, genetic engineering tools were employed to develop transgenic lines of freshwater microalga Chlorella vulgaris with a higher starch content, by up-regulating ADP-glucose pyrophosphorylase (AGPase), which is a rate-limiting enzyme in starch biosynthesis. Expression of the Escherichia coli glgC (AGPase homolog) gene in C. vulgaris led to an increase in total carbohydrate content up to 45.1% (dry cell weight, DCW) in the transgenic line as compared to 34.2% (DCW) in the untransformed control. The starch content improved up to 16% (DCW) in the transgenic alga compared to 10% (DCW) in the control. However, the content of total lipid, carotenoid, and chlorophyll decreased differentially in the transgenic lines. The carbohydrate-rich biomass from the transgenic algal line was used to produce bioethanol via yeast fermentation, which resulted in a higher ethanol yield of 82.82mg/L as compared to 54.41mg/L from the untransformed control. The in vitro digestibility of the transgenic algal starch revealed a resistant starch content of up to 7% of total starch. Faster growth of four probiotic bacterial species along with a lowering of the pH of the growth medium indicated transgenic alga to exert a positive prebiotic effect. Taken together, the study documents the utilization of genetically engineered C. vulgaris with enriched carbohydrates as bioethanol feedstock and functional food ingredients.

Connect the mulberry leaf quality and the silkworm cocoon traits through correlation and regression analysis

An experiment was conducted to study the impact on mulberry leaf quality and silkworm growth performance by the application of growth promoter. In the irrigated mulberry field experiment was carried out in Randomized Block Design layout with four replications and five treatments. Result showed that higher quality of mulberry leaves was obtained on Effective Microorganisms (EM) treated plots compared to others because beneficial microorganism in the EM enhanced the plant growth metabolism to increase their growth. As for correlation analysis, all leaf quality traits were highly significantly positive correlated with silkworm cocoon traits. In simple linear regression analysis, the mulberry leaf quality traits were significantly influenced the silkworm cocoon traits except leaf protein with cocoon weight (Adj R2 = - 0.30337) which has negative relationship between them. Highly close relationship was noticed on total carbohydrate content on larval weight (Adj R2 = 0.93655) followed by total chlorophyll content on shell weight (Adj R2 = 0.90569), silk gland weight (Adj R2 = 0.87275), ERR (Adj R2 = 0.84312) and total carbohydrate content on shell weight (Adj R2 = 0.84606). Thus, the total chlorophyll and carbohydrate content might be a good trait and mostly responsible for producing good quality cocoons.

Drying behavior and nutritional quality of bitter‐gourd slices dried in a solar dryer with tray and skewer arrangement

AbstractIn this investigation, we assessed the efficacy of an indirect‐type solar drying system that utilized skewers and rack arrangements, comparing it to a conventional drying cabinet equipped with trays for dehydrating bitter gourd slices. We examined the influence of pretreatment methods and loading quantities on the solar drying process for bitter gourd slices in both configurations. The drying behavior of various combinations was scrutinized, and the physicochemical attributes of the dried bitter gourd samples, including total phenolic content, total flavonoid content, total chlorophyll content, antioxidant capacity, ascorbic acid content, and color, were analyzed. Rehydration characteristics, such as rehydration ratio, coefficient of rehydration, percent water in the rehydrated sample, and hardness of the rehydrated sample, were also determined. The bitter gourd slices achieved a final moisture content of 6.84%–8.27% wb after drying from an initial range of 88%–90% wb, within a total elapsed time of 28–55 h. The solar drying cabinet utilizing skewers exhibited enhanced efficiency, featuring reduced drying time and superior product quality compared to the tray‐equipped drying cabinet. This improved performance is ascribed to enhanced hot air circulation over the produce surface, facilitated by the uniform spacing between skewers on racks within the drying chamber.

HSVd elimination from tomato using cryotherapy combined with exogenous HSVd-targeting ds-sRNA Application

The development of viroid elimination technology is essential in tomato (Solanum lycopersicum) production. Combining long-term cold therapy and meristem culture is commonly used; however, tomatoes cannot withstand cold. How the Hop stunt viroid (HSVd) affects the growth potential, activities of oxidoreductases and the contents of soluble matters and total chlorophyll was studied. HSVd-targeting double-stranded small RNAs (ds-sRNAs) were synthesized to match different regions of its genome (ds-sRNAs-H1∼H5), and their permeability in plants with HSVd inhibition effects was tested. The HSVd infected tomato shoots were exogenously treated with ds-sRNAs, and the meristems were thereafter taking from treated shoots and processed using leaf primordia-free meristem culture and cryotherapy. HSVd inhibited tomato stem elongation, leaf growth and root development, it elevated the oxidoreductase activity and disturbed the contents of soluble protein, soluble sugar and chlorophyll content. Exogenous ds-sRNAs can penetrate all types of cells to induce HSVd gene silencing in shoot terminals, causing the accumulation of miRNAs produced by HSVd and differed expression of gene silencing-related genes. The shoot terminal ex vivo treated with ds-sRNA which targeting the central conserved region of HSVd genome (H3) gained the highest inhibition of HSVd titer after 36 h of feeding. Leaf primordia-free meristem culture couldn't obtain viroid-free regenerants from ds-RNA-H3 treated meristems, whereas in cryotherapy, meristems soaked in PVS2 for 15 and 20 min resulted viroid elimination of 6.7 % and 10.0 %, 5 and 10 min of PVS2 caused 0 % HSVd elimination. Growth and physiological state of HSVd-eliminated plants recovered to those of healthy controls. The results provide theoretical and technical basis for the sustainable development of the tomato pathogen-free plant industry.

Yield Trait and Stability of Chickpea Genotypes for Intensification of Drought‐Prone Rice Fallows of South Asia

ABSTRACTThere is a need for increasing cropping intensity in South Asia including India to ensure food security of burgeoning population. Accordingly, increasing cropping intensity in rainfed rice fallows can be a futuristic strategy. Identification of suitable cultivar and exploration of genetic variability of specific crops/traits are imperative for genetic improvement, drought resistance and yield gain in rice fallows. We evaluated the morphophysiological, yield traits and stability of 15 chickpea genotypes in randomised complete block design for three consecutive years on a drought‐prone rainfed condition of Fluvisol in Kanpur, India. Among genotypes, ‘IPC 2014‐55’, ‘IPC 2015‐44’ and ‘IPC 2011‐92’ had 2%–10% higher relative water content (RWC) over ‘ICC‐92944’ (check cultivar). These genotypes did not differ for total chlorophyll content, root dry weight and nodule dry weight with ‘ICC‐92944’ and ‘KWR 108’ (wider adaptable cultivar of the region). The nitrogen balance index was higher in ‘IPC 2011‐92’, ‘IPC 2014‐88’ and ‘IPC 2014‐55’ by 5%–44% over check cultivar (p < 0.05). The membrane stability index was higher for ‘IPC 2014‐55’ (30%, p < 0.05) and ‘IPC 2011‐92’ (17%, p < 0.05) than ‘ICC‐92944’. ‘IPC 2011‐92’, ‘IPC 2014‐88’ and ‘IPC 2014‐55’ (3 years mean) had 3%–24% higher plant dry weight than ‘ICC‐92944’. Notably, ‘IPC 2014‐55’, ‘IPC 2015‐44’, ‘IPC 2014‐88’ and ‘IPC 2011‐92’ had higher yield attributes such as pods plant−1 by 9%, grain weight plant−1 by 13% and 100‐seed weight by 3% than ‘ICC‐92944’ and ‘KWR 108’ (mean of years). These genotypes had higher mean seed yield than ‘ICC‐92944’ by 23%–42% and ‘KWR 108’ by 7%–23% (p < 0.05). The yield of ‘IPC 2014‐55’, ‘IPC 2015‐44’, ‘IPC 2014‐88’ and ‘IPC 2011‐92’ were stable over years across variable soil and environmental condition as indicated by the genotype × year biplot. Membrane stability index, pods plant−1 and 100‐seed weight were the determinants for increased seed yield of chickpea under drought‐prone condition. Evidently, genotype ‘IPC 2014‐55’, ‘IPC 2015‐44’, ‘IPC 2014‐88’ and ‘IPC 2011‐92’ were better under rainfed rice fallows. These genotypes could be tested under specific drought condition for developing varieties and promoted in rice fallows of South Asia for yield advantage and drought resistance.

Physiological and anatomical responses of Passiflora tripartita var. mollissima (Passifloraceae) in water deficit

Introduction: Passiflora tripartita var. mollissima (banana passionfruit) is one of the most promising exotic tropical fruits from the diversity of the Passifloraceae family in South America, because of its organoleptic properties and antioxidant activity. Objective: To evaluate the physiological and anatomical responses of banana passionfruit plants under water deficit to better understand the mechanisms that mitigate this stress and affect the production of crops subject to climate change and global warming. Methods: Three-month-old seedlings of banana passionfruit were subjected to a soil water deficit through an irrigation reduction at 70 % for 49 days under greenhouse conditions. Morphology (leaf area, height, and number of leaves) and physiological (stomatal conductance, Fv/Fm, total chlorophyll content) measurements were made through time, and after the irrigation treatments were measured biomass parameters and anatomical foliar traits. Results: The plants experienced a decrease in height, leaf area, number of leaves, leaf area index, and relative water content, that are common responses in plants subjected to reduced irrigation. Additionally, the plants exhibited certain mechanisms that can be attributed to water deficit tolerance such as higher root: shoot ratio, stomatal closing, an increase in stomatal density, a reduction in mesophyll tissue thickness, and a decrease in the number of vessels and its diameter as they enable the banana passionfruit to reduce water loss and decrease the probability of cavitation in xylem vessels. Conclusions: banana passionfruit plants could implement strategies against water scarcity, allowing them to survive and endure challenging environmental conditions

Transgenic tobacco plants overexpressing a wheat salt stress root protein (TaSSRP) exhibit enhanced tolerance to heat stress.

Heat stress is a detrimental abiotic stress that limits the development of many plant species and is linked to a variety of cellular and physiological problems. Heat stress affects membrane fluidity, which leads to negative effects on cell permeability and ion transport. Research reveals that heat stress causes severe damage to cells and leads to rapid accumulation of reactive oxygen species (ROS), which could cause programmed cell death. This current study aimed to validate the role of Triticum aestivum Salt Stress Root Protein (TaSSRP) in plants' tolerance to heat stress by modulating its expression in tobacco plants. The Relative Water Content (RWC), total chlorophyll content, and Membrane Stability Index (MSI) of the seven distinct transgenic lines (T0 - 2, T0 - 3, T0 - 6, T0 - 8, T0 - 9, T0 - 11, and T0 - 13), increased in response to heat stress. Despite the fact that the same tendency was detected in wild-type (WT) plants, changes in physio-biochemical parameters were greater in transgenic lines than in WT plants. The expression analysis revealed that the transgene TaSSRP expressed from 1.00 to 1.809 folds in different lines in the transgenic tobacco plants. The gene TaSSRP offered resistance to heat stress in Nicotiana tabacum, according to the results of the study. These findings could help to improve our knowledge and understanding of the mechanism underlying thermotolerance in wheat, and the novel identified gene TaSSRP could be used in generating wheat varieties with enhanced tolerance to heat stress.

Chlorophyll content estimation and ripeness detection in tomato fruit based on NDVI from dual wavelength LiDAR point cloud data

Tomato (Solanum lycopersicum L.) is a climacteric fruit exhibiting the ripening pattern with change in colour from green to red. Normalized difference vegetation index (NDVI) derived from spectral-optical analysis has been previously employed in ripeness analysis of various fruits based on its strong correlation to ripening-related chlorophyll content. In this study, light detection and ranging (LiDAR) laser scanner was applied to gain 3D tomato point clouds aimed at estimating the spatially resolved chlorophyll content and ripeness class of tomato fruit. Freshly harvested tomatoes, capturing six ripeness classes (mature green, breaker, turning, pink, light red, and red) according to USDA colour standard, were analysed using a linear conveyor mounted sensor system consisting of two LiDAR units measuring position and return signal strength intensity at 660 nm and 905 nm. Fruit point clouds were pre-processed including geometric correction considering the curvature of each tomato sample, removing highest intensity areas at the specular highlighted spots, calibration of intensity values using standard black and white colour coated boards, and calibration on attenuation of the opaque samples. Particularly, for gaining the attenuation coefficient (μ), 9 opaque synthetic models with known attenuation were used. Obtained μ660 and μ905 were merged to gain NDVILiDAR. Chemically analysed chlorophyll content of tomato samples was correlated to μ660 and NDVILiDAR, whereas low correlation appeared for μ905 with a coefficient of determination (R2) of 0.58, 0.60, and 0.06, respectively. Regression models were used to estimate the total tomato chlorophyll content and the lowest root mean square error (RMSE) was found for μ660 (RMSE = 4.97 mg (100 g dry mass)−1) followed by NDVILiDAR (RMSE = 5.22 mg (100 g dry mass)−1), and μ905 (RMSE = 53.50 mg (100 g dry mass)−1). Histograms of NDVILiDAR and μ660 were extracted from each tomato point cloud and utilized to construct PLS-DA model for tomato ripening class prediction considering the spatial chlorophyll distribution. The overall accuracies for NDVILiDAR and μ660 were 70 % and 68 %, respectively, in leave-one-out cross-validation for visually defined colour classes. The LiDAR-based approach could support selective detection of ripe fruit in robots for harvesting and postharvest handling.

ROLE OF PHOTOSYNTHETIC STABILITY AND PHYSIO-CHEMICAL ATTRIBUTES IN THE SELECTION OF IMPROVED COTTON GENOTYPES IN ACTUAL FIELD CONDITIONS

The current experimental study was conducted at Cotton Research Station, Bahawalpur during the cotton growing in 2022. The study was designed to evaluate the elite cotton genotypes based on their photosynthetic sustainability and other physiochemical attributes which could help in framing a selection criterion for the development of improved carton genotypes. The results revealed that highly significant variations were present among the elite cotton genotypes for studied morpho-physiological, photosynthetic and biochemical traits i.e., CLCV incidence percentage, number of bolls, sympodia per plant, boll weight, net photosynthetic rate, total chlorophyll contents, transpiration rate, stomatal conductance, sugar contents, total phenolic contents, ascorbic acid and seed cotton yield, which could be used in devising selection criteria for this election of parental material to be used in the hybridization program. Furthermore, the correlation coefficient analysis unveiled the strong correlation of seed cotton yield with transpiration rate, sugar contents, stomatal conductance, net photosynthetic rate, ginning out turn and sympodia per plant whereas a strong negative correlation was found for total phenolic contents. These results were also verified through the mean bar graphs and biplot graphs which show that these traits have the highest influence on seed cotton yield under the optimal condition.

Evaluation of Antimicrobial and Antioxidant Potential of Oxalis corymbosa Extracts.

This work aimed to assess the antioxidant and antimicrobial properties of Oxalis corymbosa extracts. Biochemical analyses were conducted on various plant parts, utilizing enzymatic and non-enzymatic assays. Parameters such as total soluble protein, chlorophyll, and carotenoid contents were also evaluated to elucidate the role of bioactive chemical compounds. The antimicrobial screening of extracts was performed against the bacterial and fungal strains Escherichia coli, Staphylococcus aureus, and Candida albicans, respectively. Results indicated that chlorophyll a, chlorophyll b, total chlorophyll, carotenoid content, anthocyanin content, catalase, peroxidase, and superoxide dismutase were most abundant in the O. corymbosa leaves. Moreover, total ascorbate peroxidase content, total phenolic content, and total flavonoid content were found to be higher in the roots compared to other parts. High-performance liquid chromatography analysis identified chlorogenic acid as the major component, followed by gallic acid, caffeic acid, quercetin, and salicylic acid. Regarding antibacterial potential, each extract exhibited significant activity, with methanolic and ethyl acetate extracts demonstrating the maximum inhibition zone against S. aureus and E. coli, respectively. These findings highlight the substantial antioxidant and antibacterial potential of different parts of O. corymbosa, suggesting their promising applications as ingredients in various nutraceutical products.

Microplastics in soil affect the growth and physiological characteristics of Chinese fir and Phoebe bournei seedlings

Pot experiments were conducted using Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) and Phoebe bournei (Hemsl.) Yang) to investigate whether soil microplastics adversely affect the nurturing and renewal of plantations. Microplastics composed of polyethylene and polypropylene with a size of 48 μm were used. The treatments included a control group (without microplastics) and groups treated with microplastic concentrations of 1% and 2% (w/w). The effects of microplastics on the growth, photosynthetic pigments in leaves, antioxidant systems, and osmotic regulation substances of the seedlings were analysed by measuring the seedling height, ground-line diameter growth, chlorophyll (chlorophyll a, chlorophyll b, and total chlorophyll) contents, antioxidant enzyme (superoxide dismutase, peroxidase, catalase) activities, and malondialdehyde, soluble sugar, and soluble protein levels. The results indicated that treatment with 1% polyethylene microplastics increased the chlorophyll a, total chlorophyll, and soluble protein contents in the leaves of both types of seedlings while inhibiting superoxide dismutase and peroxidase activities in P. bournei seedlings. Treatment with 2% polyethylene or polypropylene microplastics suppressed the chlorophyll a, chlorophyll b, and total chlorophyll contents; superoxide dismutase, peroxidase, and catalase activities; and soluble sugar and soluble protein levels in the leaves of both types of seedlings, resulting in reduced growth in terms of height and ground-line diameter. The physiological effects of polyethylene microplastics were more evident than those of polypropylene at the same concentration. The results demonstrated that microplastics can affect photosynthesis, the antioxidant system, and osmotic regulation in Chinese fir and P. bournei seedlings, thereby inhibiting their normal growth and development. Exposure to 1% (w/w) microplastics triggered stress responses in seedlings, whereas 2% (w/w) microplastics impeded seedling growth.

Methyl jasmonate advances fruit ripening, colour development, and improves antioxidant quality of ‘Yoho’ and ‘Jiro’ persimmon

Methyl jasmonate (MJ) has potential to regulate fruit ripening and quality. ‘Yoho’ and ‘Jiro’ persimmons were sprayed with MJ (0, 2, 4, and 6 mM), four weeks before anticipated harvest to evaluate its effects on fruit colour and bioactive compounds. Preharvest MJ application significantly improved fruit colour with increased a*, b*, chroma, and colour index. The MJ 6 mM application had significantly enhanced soluble solids content (SSC), reduced total chlorophyll content in peel and pulp, and soluble and total tannins in persimmons. MJ treatments exhibited higher contents of total phenolics, flavonoids, carotenoids, and antioxidant activities. Additionally, MJ treatments enhanced the activities of shikimate dehydrogenase (SKDH), phenylalanine ammonia-lyase (PAL), catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and lipoxygenase (LOX) enzymes. Overall, pre-harvest MJ application at 6 mM four weeks before anticipated harvest could be useful for advancing colour and improving bioactive compounds in ‘Yoho’ and ‘Jiro’ persimmons.

Feasibility of composted green waste amended by vermiculite and earthworm casts as the growth media for three common ornamental plants.

This study demonstrated the effects of adding specific proportions of vermiculite (VMT: 0%, 10%, and 20%) and earthworm casts (EWCs: 0%, 10%, and 20%) on the physico-chemical properties of composted green waste (CGW), and the impacts of amended CGW as growth media on the growth of three common ornamental plants (Dahlia pinnata Cav. [dahlia], Centaurea cyanus L. [cornflower], and Consolida ajacis[L.] Schur [delphinium]). Compared with Treatment T1 (CK), the addition of 10% VMT and 20% EWCs greatly (p < 0.05) increased the total porosity, aeration porosity, water-holding porosity, total nitrogen, available phosphorus, available potassium, and organic matter of CGW by 9%, 35%, 4%, 18%, 27%, 13%, and 33%, respectively. In addition, this pattern increased (p < 0.05) the total fresh biomass, total chlorophyll content, and root length of dahlias by 9%, 19%, and 27%, respectively; those of cornflowers by 17%, 30%, and 29%, respectively (p < 0.05); and those of delphiniums by 23%, 14%, and 63%, respectively. Therefore, the amended CGW supplemented with 10% VMT and 20% EWCs was an ideal growth medium for the three plants.

Foliar Nutrient Application with Macro and Micro Nutrients Alters Yield and Total Antioxidative Capacity of Bhendi (Abelmoschus esculentus)

Background: Plant nutrient deficiency is the major challenge in agriculture results in lower crop yield and quality. This may lower the farmer’s income by reducing marketable value. In other hand, eating nutrient dense vegetables is the most cost effective strategy to combat malnutrition. The efficiency of inorganic micronutrients applied into soils is low as they become easily fixed to soil particles. Hence, exogenous application of nutrients is an effective option to enhance plant nutrient use efficiency. Methods: This study was conducted at SRM College of Agricultural Sciences, Chengalpattu, Tamil Nadu during 2022-2023 to alleviate nutrient insufficiency challenges in bhendi variety Senora by using Randomized block design with seven different treatments viz., T1- Control, T2- Nutrient consortia 1 (N + K + B + Fe+ Mn + Zn @ 1% ), T3- Nutrient consortia 2 (N + K + Fe+ Cu + B @ 1%), T4- Nutrient consortia 3 (N + K + Mg + B + Fe + Zn @ 1%), T5- Nutrient consortia 4 (T2 + NAA + SA@ 1%), T6- Nutrient consortia 5 (T3 + NAA + SA @ 1%), T7- Nutrient consortia 6 (T4 + NAA + SA @ 1%), sprayed at 30, 45 and 60 days after sowing. Result: Various physiological, biochemical, yield and quality parameters were measured to evaluate the effects of Nutrient consortia. The results of field experiment strongly improved that the eight biochemical parameters viz., chlorophyll a, chlorophyll b, total chlorophyll content, chlorophyll a/b ratio, chlorophyll stability index, leaf soluble protein, lipid peroxidation by melondialdehyde and proline content. The yield and quality traits such as number of fruits per plant, total fruit yield, total phenolics and total antioxidants activity were significantly increased through application of Nutrient consortia 6 followed by Nutrient consortia 4. So this can be used as yield and quality enhancer formulation of bhendi crops.

Assessment and comparative analysis of the antioxidant capacity of some food waste for fish oils

The aim of the present study was to compare the antioxidant capacities of butylated hydroxyl anisole and tocopherol with polar extracts obtained from some food waste such as date seed, walnut shell, sesame hull, spent coffee and spent black tea. In pursuit of this objective, crude fish oil was employed as a control sample, with all extracts added at a concentration of 200 ppm, equivalent to BHA. The acid, peroxide, conjugated dien, viscosity, total chlorophyll and carotenoid contents in the fish oil were monitored during the storage period (60 days) at 25 °C and 35 °C. The date seed and walnut extracts presented higher antioxidants and oxidative stability than the others at the end of the storage period at both temperatures. The findings of the present study showed that the extracts obtained from food wastes/by-products could be evaluated as a natural source of antioxidants, especially for oils which are highly susceptible to oxidation.

Morphological and Pigment Responses to Far-Red and Photosynthetically Active Radiation in an Olive Cultivar Suitable for Super-High-Density Orchards.

Plant density is increasing in modern olive orchards to improve yields and facilitate mechanical harvesting. However, greater density can reduce light quantity and modify its quality. The objective was to evaluate plant morphology, biomass, and photosynthetic pigments under different red/far-red ratios and photosynthetically active radiation (PAR) combinations in an olive cultivar common to super-high-density orchards. In a greenhouse, young olive trees (cv. Arbequina) were exposed to low (L) or high (H) PAR with or without lateral FR supplementation (L+FR, L-FR, H+FR, H-FR) using neutral-density shade cloth and FR light-emitting diode (LED) modules. Total plant and individual organ biomass were much lower in plants under low PAR than under high PAR, with no response to +FR supplementation. In contrast, several plant morphological traits, such as main stem elongation, individual leaf area, and leaf angle, did respond to both low PAR and +FR. Total chlorophyll content decreased with +FR when PAR was low, but not when PAR was high (i.e., a significant FR*PAR interaction). When evaluating numerous plant traits together, a greater response to +FR under low PAR than under high PAR appeared to occur. These findings suggest that consideration of light quality in addition to quantity facilitates a fuller understanding of olive tree responses to a light environment. The +FR responses found here could lead to changes in hedgerow architecture and light distribution within the hedgerow.

Effect of potassium chloride-induced salt stress on bacoside A biosynthesis in Bacopa monnieri (L.) grown under in vitro and in vivo conditions: a comparative study.

Bacopa monnieri L. is a highly acclaimed plant species for its diverse pharmaceutical properties and is mostly found in the Indian subcontinent. In this study, the effects of salt (KCl) stress on plant height, biomass, chlorophyll content, and antioxidant enzyme activities of Bacopa monnieri in both in vitro and in vivo conditions were investigated. A significant increase of up to 1.8 folds and 1.3 folds in bacoside-A content at 100mM KCl was recorded in both in vivo and in vitro grown plants, respectively. Higher salinity (> 100mM KCl) stress exerted a negative effect on plant height and plant biomass, whereas at levels ≤ 100 KCl, substantial improvement in terms of plant height (PH) and biomass (PB) was recorded in both in vivo (up to 1.6-fold and 1.8-fold high) and in vitro (up to 1.9-fold and 1.7-fold high) conditions. Total chlorophyll content and antioxidant enzyme (CAT, POD) activities were also maximum at 100mM KCl. However, at higher KCl levels (200mM), no significant increase in any of the morphophysiological parameters was recorded. Therefore, 100mM KCl was identified as the optimum salt concentration for enhancing bacoside A content, plant growth, and physiological properties in terms of antioxidant enzyme activity and chlorophyll content in B. monnieri.

Impact of NPK and Cytokinin on Growth Characteristics and Chemical Composition of Ficus Nitida Plants

Abstract The research was conducted in a greenhouse at the Department of Horticulture and Garden Engineering, College of Agriculture, University of Anbar, Ramadi City, to study the impact of NPK supplementation and CPPU cytokinin spraying on the vegetative and root growth characteristics of Ficus nitida L. The study encompassed two primary factors. The first factor comprises the NPK chemical fertilizer in four distinct concentrations: F0 (no addition), F1 (1g urea, 3g DAB, 2g potassium sulfate), F2 (2g urea, 6g DAB, 4g potassium sulfate), and F3 (3g urea, 9g DAB, 4g potassium sulfate). The second factor involves the cytokine CPPU at three concentrations: C0 (no addition), C1 (3 mg L-1), and C2 (6 mg/liter). The study was designed using a randomized complete block design (RCBD) consisting of three replications, with three pots allocated to each experimental unit. Each replicate consisted of 36 treatments, meaning the experiment contained 108 experimental units in all blocks. The results indicated a significant increase in the interaction coefficients between the study factors in plant height, leaf area, nitrogen percentage in the leaf, total chlorophyll content, root surface area, and root length.. Specifically, these parameters were measured at 21.333 cm, 30.837 cm2, 2.666%, 1.384 g, 55.223 cm2, and 13.947 cm, respectively.