Plasma Total Antioxidant Capacity Research Articles

Circulating redox status in sheep naturally infected with Trichophyton verrucosum

Trichophyton verrucosum is a zoophilic dermatophyte that causes skin inflammation. The present study aimed to evaluate the redox status in the blood of sheep clinically infected with T. verrucosum. According to clinical and mycological investigations, 48 juvenile male Balady sheep were selected in their natural habitat and divided into four groups depending on the lesion size: mild (MID), moderate (MOD), severe (SEV) T. verrucosum infection, and healthy control groups. Compared to the controls, plasma superoxide anion increased (P < 0.05) in both MOD and SEV but total peroxides (TPx) gradually increased (P < 0.05) in MID followed by MOD and SEV. Superoxide dismutase and total antioxidant capacity (TAC) were higher (P < 0.05) in MID and lower (P < 0.05) in MOD and SEV than in controls, but SEV showed lower TAC than MOD. Malondialdehyde (MDA, a lipid peroxide marker) increased (P < 0.05) in SEV than in controls, but protein carbonyl (PC, a protein peroxidation marker) was augmented (P < 0.05) as lesions progressed from mild to severe. The oxidative stress index (TPx/TAC ratio) progressively increased (P < 0.05) in MOD and SEV. The correlation of PC was positive with TPx and negative with TAC (P < 0.01). In conclusion, sheep infection with T. verrucosum is characterized by increased TPx and decreased TAC in plasma depending on the lesion area. The redox status is shifted towards the oxidizing state, particularly in MOD and SEV cases. This results in a condition of OS, which may contribute to the pathogenesis of the disease.

Iron sulfate and molasses treated anthocyanin-rich black cane silage improves growth performance, rumen fermentation, antioxidant status, and meat tenderness in goats.

This study investigated the effects of feeding anthocyanin-rich black cane treated with ferrous sulfate and molasses on animal performance, rumen fermentation, microbial composition, blood biochemical indices, and carcass characteristics in meat goats. Thirty-two Thai-native×Anglo-Nubian crossbred male goats (14.47±2.3 kg) were divided equally into two groups (n = 16) to investigate the effect of feeding diet containing 50% untreated anthocyanin-rich black cane silage (BS) vs diet containing anthocyaninrich black cane silage treated with 0.03% ferrous sulfate and 4% molasses (TBS) on average daily gain (ADG) and dry matter intake (DMI). At the end of 90 d feeding trial, the goats were slaughtered to determine blood biochemical indices, rumen fermentation, microbial composition, and carcass characteristics differences between the two dietary groups. Goats fed the TBS diet had greater ADG and ADG to DMI ratio (p<0.05). TBS diet did not affect rumen fluid pH; however, goats in the TBS group had lower rumen ammonia N levels (p<0.05) and higher total volatile fatty acid concentrations (p<0.05). Goats in the TBS group had a higher (p<0.05) concentration of Ruminococcus albus but a lower (p<0.05) concentration of methanogenic bacteria. The TBS diet also resulted in lower (p<0.05) thiobarbituric acid-reactive substances concentration but higher (p<0.05) total antioxidant capacity, superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase concentrations in blood plasma, while having no effect on plasma protein, glucose, lipid, immunoglobin G, alanine transaminase, and aspartate aminotransferase. Meat from goats fed the TBS diet contained more intramuscular fat (p<0.05) and was more tender (p<0.05). In comparison to goats fed a diet containing 50% untreated anthocyanin-rich black cane silage, feeding a diet containing 50% anthocyanin-rich black cane silage treated with 0.03% ferrous sulfate and 4% molasses improved rumen fermentation and reduced oxidative stress, resulting in higher growth and more tender meat.

Postprandial Responses of Serum Cholesterol, Glucose and Plasma Antioxidant Activity, after Intake of an Innovative High Fat Mayonnaise-Based Appetizer, Enhanced with Olive Paste, in Healthy Volunteers.

Several Mediterranean functional foods and their process by-products may exert a beneficial role on hyperlipidemia, hyperglycemia, and oxidative stress modulation, providing bioactive compounds with functional properties, contributing to possible chronic disease prevention (cardiovascular disease, metabolic syndrome, obesity, diabetes mellitus, etc.). The purpose of the present interventional study was to investigate the postprandial responses of metabolic biomarkers, after the intake of an innovative mayonnaise-based appetizer, enhanced with olive paste, in healthy volunteers. In this cross-over design, randomized and single-blind, interventional–clinical trial, 10 healthy volunteers, aged 20–30 years old, after splitting into the control group and the Mediterranean group, consumed a pasta meal rich in fat and carbohydrates (150 g), containing a mayonnaise-based appetizer or the same appetizer, enhanced with 9% olive paste. After a 1-week washout period, the subjects consumed the meals in reverse. Differences between groups on postprandial responses of total plasma antioxidant capacity according to the FRAP method, serum total cholesterol, glucose, and uric acid levels, were determined before, 30 min, 1.5 h, and 3 h after consumption. The results showed that, in comparison to the control group, consumption of the enhanced appetizer resulted in a significantly decreased total serum cholesterol and glucose levels, and also led to a significant increase in plasma total antioxidant activity, 3 h after consumption (p < 0.05). Further investigation with large prospective studies is needed to validate the current results.

Telomere Shortening and Increased Oxidative Stress in Lumbar Disc Degeneration.

Lumbar disc degeneration (LDD) contributes to low back pain. This study aimed to determine relative telomere length (RTL), oxidative stress status, and antioxidant levels and examine the relationships between RTL, oxidative stress, and the severity in LDD patients. A total of 100 subjects, 50 LDD patients and 50 healthy controls, were enrolled in the case–control study. Blood leukocyte RTL was analyzed using quantitative real-time polymerase chain reaction. Lipid peroxidation was determined by malondialdehyde (MDA) assay. Plasma 8-hydroxy 2′-deoxyguanosine (8-OHdG) values were determined using enzyme-linked immunosorbent assay. Total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP) in plasma were also measured. The LDD patients had significantly shorter telomeres than the healthy controls (p = 0.04). Blood leukocyte RTL was inversely correlated with the LDD severity (r = −0.41, p = 0.005). Additionally, plasma MDA and 8-OHdG levels were markedly greater in LDD patients than in the controls (p = 0.01 and p = 0.002, respectively). Furthermore, the plasma MDA level showed a positive correlation with the radiographic severity (r = 0.49, p = 0.001). There was a positive correlation between plasma 8-OHdG and the severity (r = 0.60, p < 0.001). Moreover, plasma TAC and FRAP levels were significantly lower in LDD patients than in the controls (p = 0.04). No significant differences in plasma TAC and FRAP were observed among the three groups of LDD severity. We found that RTL was negatively correlated with the severity while plasma MDA and 8-OHdG levels were positively correlated with the severity. These findings suggest that blood leukocyte RTL, plasma MDA, and 8-OHdG may have potential as noninvasive biomarkers for the assessment of severity in LDD.

Grape seed extract supplementation in heat-stressed preweaning dairy calves: I. Effects on antioxidant status, inflammatory response, hematological and physiological parameters

This study aimed to investigate the effects of grape seed extract (GSE) supplementation on antioxidant activity, inflammatory markers, hematological and physiological responses during the suckling period in heat-stressed calves. Sixty Holstein female calves (3 d of age, 40.6 ± 2.17 kg of BW) were randomly divided into four dietary groups. Each group consisted of 15 calves; the milk was supplemented with 0 (CON) 25 (GSE1), 50 (GSE2), and 100 (GSE3) mg/kg BW/day of GSE. On d 3, 33, and 63 blood samples were collected, rectal temperatures (RT) and respiratory rates (RR) were measured three days a week. Calves were exposed to chronic heat stress during the study period and were weaned on d 63. Plasma malondialdehyde (MDA) and tumor necrosis factor-alpha (TNF-α) concentrations decreased (P < 0.01) in all GSE supplemented groups compared to the CON group. Also, interferon-gamma (IFN-γ) concentration decreased (P = 0.03) only in calves fed GSE1. Plasma total antioxidant capacity (TAC) concentration increased (P = 0.02) in calves fed GSE3 on d 33 compared to the CON group, while the concentration of superoxide dismutase (SOD) was higher (P < 0.001) in the GSE2 and GSE3 groups compared to CON and GSE1. The hematocrit percentage, hemoglobin value, RBC, and eosinophil counts were higher (P < 0.01) in all GSE supplemented groups compared to CON. Calves fed GSE1, GSE2 and GSE3 had lower (P < 0.001) neutrophil count compared to CON on d 63. All GSE-supplemented groups had a decreased (P < 0.001) RR compared to CON, while calves fed GSE2 had a tendency to decrease (P = 0.10) in RT. It was concluded that supplementation with 50 and 100 mg/kg BW/d GSE could improve antioxidant, anti-inflammatory activities, and hematological parameters of calves against oxidative stress, and also reduce the effect of heat stress by reducing the respiratory rate.

Effect of dietary resveratrol supplementation on growth performance, antioxidant capacity, intestinal immunity and gut microbiota in yellow-feathered broilers challenged with lipopolysaccharide

Resveratrol (RES) displays strong antioxidant and anti-inflammatory properties in protecting the animals from various stressors and inflammatory injuries, but its interrelationship with the gut microbiota remained largely unclear. This study was carried out to investigate the effects of dietary RES supplementation on growth performance, antioxidant capacity, intestinal immunity and gut microbiota in yellow-feathered broilers challenged by lipopolysaccharide (LPS). A total of 240 yellow-feathered broilers were randomly assigned to four treatment groups in a 2 × 2 factorial design. The broilers were fed with the control diet or control diet supplemented with 400 mg/kg RES, followed by challenge with LPS or the same amount of saline. Dietary RES supplementation significantly alleviated the decreases in the final body weight (BW), average daily gain (ADG), and ADFI induced by LPS (P < 0.05). LPS challenge significantly increased plasma concentrations of triglyceride, high-density lipoprotein cholesterol (HDL-C), aspartate aminotransferase (AST), and cortisol levels, but decreased triiodothyronine (T3) and insulin levels (P < 0.05). Dietary supplementation with RES significantly reversed the elevated creatinine concentrations and the decreased concentrations of T3 and insulin caused by LPS (P < 0.05). Moreover, dietary RES supplementation significantly increased plasma total antioxidant capacity (T-AOC) and catalase (CAT) activities and superoxide dismutase (SOD) and T-AOC activities in jejunal mucosa and reduced malondialdehyde (MDA) concentration in the plasma (P < 0.05). The reduction in the villus height to crypt depth ratio in duodenum, jejunum and ileum and the shortening of villus height in jejunum and ileum caused by LPS were also alleviated by RES treatment (P < 0.05). Furthermore, the increased concentrations of intestinal tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β caused by LPS were significantly decreased by RES treatment (P < 0.05). Dietary RES treatment increased the mRNA expression of claudin-1, claudin-5, occludin, and zonula occludens-1 (ZO-1), and decreased mRNA expression of IL-1β, IL-8, IL-17, and TNF-α after LPS challenge (P < 0.05). Dietary RES treatments significantly decreased the dominance of cecal microbiota, and increased the Pieiou-e and Simpson index. Moreover, dietary RES supplementation increased relative abundance of UCG_ 009, Erysipelotrichaceae, Christensenellaceae_R-7_group, Anaerotruncus, RF39, and Ruminococcus while decreasing the abundance of Alistipes at genus level. Spearman correlation analysis revealed that the microbes at the order and genus levels significantly correlated with indicators of growth performance, antioxidant capacity, and intestinal health. Collectively, dietary supplementation with 400 mg/kg RES could improve growth performance and antioxidant capacity, and modulate intestinal immunity in yellow-feathered broilers challenged by LPS at early stage, which might be closely associated with the regulation of gut microbiota community composition.

Enhancing semen quality, antioxidant status and sex hormones of V-line rabbit bucks fed on supplemented diets with dried moringa leaves

This study was conducted to evaluate the impact of dried moringa leaves (DML) on semen quality, immunoglobulin measurements, blood metabolic analysis, antioxidant status and sex hormones of V-line rabbit bucks. Twenty-four mature rabbit bucks of 7-8 months-old of proven fertility with an average weight (2.8 ± 0.4 kg) were classified into four treatments (basal diet with 0, 750, 1500 and 3000 mg DML/kg diet) and 6 bucks per treatment were used. Seminal plasma cholesterol (p = 0.013) and triglycerides (p = 0.0001) of all moringa supplemented rabbits were higher than the control. Moringa leaves enhanced (p < 0.05) serum testosterone, follicle stimulating hormone and luteinizing hormone. Total antioxidant capacity, superoxide oxidase, glutathione peroxidase and catalase in seminal plasma of moringa rabbits were higher (p < 0.05) than the control. Serum total lipid (p = 0.002), cholesterol (p = 0.008), triglycerides (p = 0.019) and blood urea (p < 0.05) were lower in DML rabbits. Moringa leaves improved total antioxidant capacity (p = 0.0001), glutathione peroxidase (p = 0.0001) and superoxide oxidase (p = 0.037) in rabbits versus control. Rabbits consuming DML had higher (p < 0.05) immunoglobulin IgG and IgM than control. Results suggest that DML improved rabbits’ antioxidant, and immunological status, semen quality, and sex hormones, but DML (750 mg/kg diet) is recommended because it was more effective than the other two high doses.

Cardioprotective Role of Swertiamarin, a Plant Glycoside Against Experimentally Induced Myocardial Infarction via Antioxidant and Anti-inflammatory Functions.

The study examined the protective effects of swertiamarin on rats with experimentally induced myocardial infarction. Three to six week-old male albino Wistar rats were used in this study and experimental myocardial infarction (MI) was induced using isoproterenol. Our results showed that swertiamarin restored the alteration in heart weight, body weight, and heart weight/tibia length ratio of MI-induced rats to basal levels significantly (p < 0.05). Swertiamarin significantly (p < 0.05) restored the levels of cardiac pathophysiological marker creatine kinase (CKMB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine transaminase (ALT), and cardiac troponin I (cTn-1) to near normalcy in MI-induced rats. Levels of oxidative stress markers malondialdehyde (MDA), protein carbonyls (PC), and levels of Vitamin C and Vitamin E were significantly (p < 0.05) reverted to near basal levels in MI-induced rats by swertiamarin. Levels of the antioxidant glutathione (GSH) and antioxidant enzymes which include superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-s-transferase (GST), glutathione reductase (GR), and plasma total antioxidant capacity (TAC) were (p < 0.05) brought to near normalcy in MI-induced rats by swertiamarin. Levels of sodium (Na), potassium (k), and calcium (Ca) ATPases were significantly (p < 0.05) restored to near normalcy in MI-induced rats by swertiamarin. Status of pro-inflammatory cytokines including tumor necrosis factor (TNF-α), interleukin-6 (IL-6), and histological aberrations were also significantly (p < 0.05) restored to near normalcy in MI-induced rats by swertiamarin. Together, our results concluded that swertiamarin exerts significant cardioprotective functions in experimental MI in rats.

Role of ultraviolet ray filters and antioxidants in the prevention of DNA damage and oxidative stress associated with phototherapy in jaundiced term neonates

Background Phototherapy induces DNA damage by direct and indirect (oxidative) effects, which are prevented by ultraviolet (UV) filters and antioxidants. Aim Our goal was to assess DNA damage and oxidative stress associated with phototherapy and to prove the efficacy of UV filters and antioxidants for the prevention of DNA damage. Patients and methods The study included 160 jaundiced neonates who had been exposed to phototherapy for at least 48 h. The neonates were divided into four groups, 40 neonates in each group. The first (control) group received phototherapy only; the second group received antioxidants before and during phototherapy; the third group received phototherapy under an umbrella of UV filters; and the fourth group received phototherapy under both UV filters and antioxidants. DNA damage was assayed by the comet assay. Plasma total antioxidant capacity and total oxidant status levels were also measured and then oxidative stress index was calculated for all the four groups before and 48 h after phototherapy. Results The first group showed significant DNA damage accompanied with severe deterioration in all oxidative stress parameters by about 19%; the second group showed a decreasing trend as regards DNA damage and oxidative stress parameter deterioration to about 9%; the third group showed a more decreasing trend than group II to about 5%; but on the other hand, the fourth group showed complete DNA protection from damage with no changes in oxidative stress parameters. Conclusion Phototherapy causes DNA damage that can be completely prevented by combined concurrent use of UV filters and antioxidants.

The Effects of Purple Corn Pigment on Growth Performance, Blood Biochemical Indices, Meat Quality, Muscle Amino Acids, and Fatty Acids of Growing Chickens.

Simple SummaryAnthocyanin is a large type of polyphenol compound that is a strong natural antioxidant and widely exists in natural plant and food fields. Specifically, anthocyanins can relieve oxidative stress by scavenging oxygen free radicals to improve meat quality and extend the shelf life of meat products. Chicken meat contains high-quality protein and other substances important in the human diet and has become quite popular among consumers. Therefore, anthocyanin is a natural pigment that has been extremely valuable. We hypothesized that anthocyanin-rich purple corn pigment can improve growth performance, enhance antioxidant activity, and improve meat quality, muscle amino acid, and fatty acid profiles in chickens. The results of the current study indicate that the consumption of anthocyanin-rich purple corn pigment by growing chickens has the potential to increase growth performance, enhance antioxidant and immune capacities, heighten meat quality, and improve essential amino acids, umami amino acids, and unsaturated fatty acids in muscle.This study investigated the effects of dietary supplementation with different levels of purple corn pigment (PCP) on the growth performance, blood biochemical indices, meat quality, muscle amino acids, and fatty acids of growing chickens. A total of 288 (8 weeks of age) growing Chishui black-bone chickens (body weight, 940 ± 80 g; mean ± standard deviation) were randomly divided into 4 groups using a completely randomized design. The four diet groups were as follows: (1) control, basal diet; (2) treatment 1, treatment 2, and treatment 3, which were basal diet with 80, 160, and 240 mg/kg PCP, respectively. The results showed that compared with the control group, the feeding of anthocyanins significantly (p < 0.05) increased the average daily feed intake and average daily gain in chickens. Moreover, chickens receiving 80 mg/kg PCP significantly increased (p < 0.05) plasma total antioxidant capacity, superoxide dismutase, glutathione peroxidase, catalase, high-density lipoprotein cholesterol, and albumin concentrations relative to the control group. For meat quality, dietary supplementation with PCP significantly (p < 0.05) reduced the drip loss and water loss rate in breast muscle. Additionally, chickens receiving PCP tended to increase (p < 0.05) the levels of most individual amino acids, essential amino acids, and umami amino acids in the muscle. Specifically, the addition of 80 mg/kg PCP significantly improved (p < 0.05) total polyunsaturated fatty acids in chicken muscle. Accordingly, the consumption of anthocyanin-rich PCP by the growing chickens had the potential to increase the growth performance, enhance antioxidant and immune capacities, increase meat quality, and improve essential and umami amino acids as well as unsaturated fatty acids in the muscle.

Alpha-lipoic acid administration affects psychological status and markers of inflammation and oxidative damage in patients with type 2 diabetes and coronary heart disease.

This investigation was performed to assess the effects of alpha-lipoic acid (ALA) supplementation on psychological status and markers of inflammation and oxidative damage in patients with type 2 diabetes mellitus (T2DM) and coronary heart disease (CHD). This randomized, double-blind, placebo-controlled trial was performed in 60 patients with T2DM and CHD, aged 45-85 years. Patients were randomized into two groups to receive either 600mg/day ALA (n = 30) or placebo (n = 30) for 12 weeks. ALA supplementation significantly decreased Beck Depression Inventory index (BDI) (-5.1 ± 3.5 vs. -1.1 ± 4.8, P = 0.001) when compared with the placebo. ALA supplementation resulted also in a significant reduction of serum high sensitivity C-reactive protein (hs-CRP) (-0.8 ± 1.4 vs. +0.5 ± 0.6mg/L, P < 0.001) and malondialdehyde (MDA) (-0.3 ± 0.2 vs. -0.1 ± 0.3 µmol/L, P = 0.003), and a significant increase in plasma total antioxidant capacity (TAC) levels (+ 26.8 ± 36.0 vs. -4.6 ± 43.4 mmol/L, P = 0.007) when compared with the placebo. ALA intake upregulated transforming growth factor beta (TGF-β) (P = 0.03) and downregulated gene expression of interleukin-1 (IL-1) (P = 0.001) in peripheral blood mononuclear cells of patients with T2DM and CHD as well. ALA supplementation for 12 weeks in patients with T2DM and CHD had beneficial effects on BDI, hs-CRP, TAC, MDA values, and gene expression of IL-1 and TGF-β. The online version contains supplementary material available at 10.1007/s40200-022-01031-1.

The Effect of an Anti-inflammatory Diet on Periodontal Health in Adult Patients in the Dental Setting

ObjectivesTo explore findings that answer the clinical question, “Among adult patients (>18 years old) who received care in a dental setting, what is the effect of an anti-inflammatory diet on measures of periodontal health?” MethodsAn electronic literature search was conducted using PubMed, CINAHL, Cochrane, EBSCOhost, and Biomed Central. The initial search was broad and utilized keywords including “dental clinics,” “diet intake,” and “diet education” in order to find studies that investigated the effect of diet interventions and education in the dental setting. Filters and exclusion criteria were applied to further limit to randomized controlled trials (RCTs) that assessed the effects of an anti-inflammatory diet on periodontal outcomes. The search generated 1755 articles, 4 of which were reviewed in full text, of which 2 were then excluded. Via hand search, 2 additional articles were found, resulting in 4 RCTs that met the inclusion criteria to answer the clinical question. ResultsAll 4 RCTs included components of an anti-inflammatory diet intervention; 3 included similar anti-inflammatory diet recommendations, while 1 included personalized recommendations with anti-inflammatory components. Education was provided and compliance was monitored in all studies. Three of the studies assessed changes in markers of gingival health and found significant improvements in some outcomes of interest; however, these outcomes did not improve consistently across studies. In the first study, both gingival bleeding index (GI) and bleeding on probing (BOP) decreased significantly in the experimental group. In the second study, only GI decreased significantly in the experimental group. In the third study, there were no significant changes in GI or BOP in the experimental group, however, there was a significant increase in plasma total antioxidant capacity. The last study assessed changes in bacterial count of dental plaque and saliva and found significant reductions in some bacterial counts in the experimental group following an anti-inflammatory diet, compared to the control group. ConclusionsFollowing an anti-inflammatory diet with appropriate dietary guidance provided in a dental setting may improve gingival health. More research on the effects of an anti-inflammatory diet is necessary to further examine its clinical impact on gingival health. Funding SourcesNone.

Local and Systemic Effects of Green Coffee Extract (Coffea canephora) in Animal Model of Colorectal Carcinogenesis

ObjectivesEvaluate the local and systemic effects of aqueous extract of green coffee (GCE) in colorectal carcinogenesis (CRC) induced in rats. MethodsAdult Wistar rats (n = 48) were divided into two groups (C and T, n = 24). Group T was induced to CRC with 1,2-dimethylhydrazine (55 mg/kg, subcutaneously, weekly) for 5 weeks, and during this period all animals received standard AIN93-M diet. From week 6 to 15, the animals were divided into 4 groups (n = 12): C (healthy control fed AIN93-M diet), T (CRC- induced, fed AIN93-M diet), CGC (healthy control, fed AIN93-M diet + GCE), TGC (CRC-induced, fed AIN93-M diet + GCE). At 10th week, the pre-neoplastic lesions were evaluated in 4 rats from each group. The remaining rats (n = 8) were feed their experimental diet until the 15th week. In the last week, 24 h-urine collection was performed for intestinal permeability analysis using lactulose (100 mg/kg) and mannitol (50 mg/kg) administration. Afterwards, blood samples were collected to the analyses of plasma cytokines (TNF-alpha, interleukins IL-6, 10 and 12) and total antioxidant capacity (TAC). Large intestine content was collected for pH and secretory immunoglobulin A (sIgA) analyses. The microscopic lesions were evaluated in the colorectal mucosa. ResultsTGC group had the highest aberrant crypt foci (ACF) count, 51% higher compared to the T group. Intestinal permeability was increased in the T group. There was no significant difference in the quantification of cytokines TNF-alpha, IL-6, 10 and 12. Both TGC and T showed high values of TAC. Intestinal pH was significantly higher in CGC group compared to TGC. There was no significant difference in the quantification of sIgA between groups. The reduction of malignant neoplastic changes was 42% and of benign changes was 66% and there was a reduction of 33.33% and 20% of hyperplasia and dysplasia, respectively, in TGC compared with T group. ConclusionsGCE was beneficial for intestinal permeability and controlled the development of neoplasms in the colorectal mucosa, without interfering with systemic inflammation, reinforcing that GCE reduces the risk of developing the CRC. Funding SourcesThis study was financed by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and FAPES (Fundação de Amparo à Pesquisa e Inovação do Espírito Santo), Brazil.

Shielding effect of melatonin improves seminal quality and oxidative stress indices during chilled storage of ram semen.

Supplementation of antioxidant to semen extender maintains seminal quality by reducing oxidative stress during preservation time period. The main aim of this study was to investigate the effect of different concentrations of melatonin supplementation on liquid storage of Magra ram semen. This study was performed on adult Magra ram (n = 8), and seminal ejaculates (48) were collected and evaluated for various macroscopic and microscopic seminal quality parameters for further processing. After preliminary evaluation, ejaculates of each collection session were mixed and divided into four equal aliquots. All the aliquots were diluted (1:10) with Tris-citric fructose egg yolk extender contained sans melatonin served as control, whereas the other three aliquots were supplemented with 0.5, 1 and 2mM MLT which were grouped as MLT0.5, MLT1 and MLT2, respectively. Thereafter, the samples were stored at 4 ºC for 72h, and various seminal parameters (individual sperm progressive motility, viability, abnormalities, plasma membrane functionality) along with oxidative stress parameters (total antioxidant capacity (TAC), malondialdehyde (MDA)) were evaluated at 0, 24, 48 and 72h of preservation. The results indicated that the mean percent values for progressive sperm motility, viability, plasma membrane functionality and TAC were significantly higher (p < 0.05) in treated groups with the highest values in MLT1 group. Significantly lower (p < 0.05) percentage of total sperm abnormalities and MDA level was observed in treatment groups compared to control group. The results report that supplementation of 1mM melatonin efficiently maintains the seminal quality and ameliorates oxidative stress during preservation at 4 ºC to 72h.

Receptor of advanced glycation end product (RAGE) polymorphism and oxidative status in Hashimoto’s thyroiditis in Egyptian female patients: case control study

BackgroundHashimoto's thyroiditis is the most widespread autoimmune illness targeting a specific organ. "Redox homeostasis" is achieved when the production of Reactive Oxygen Species and their elimination are in balance. Advanced glycation end products (AGEs) are formed when glucose and/or α-oxaloaldehydes react non-enzymatically with the amino groups of lipids, proteins, and DNA. Nowadays, many studies are concerned with AGEs, the polymorphisms of their receptors, and their association with increased risk of HT. However, few studies investigated the role of receptors of advanced glycation end product (RAGE) SNP in Egyptian females.ObjectiveThe goals of this investigation were to ascertain whether oxidative stress plasma malondialdehyde (MDA) and total antioxidant capacity (TAC) were associated with HT, in addition, to assess the association of RAGE polymorphisms (− 374 T > A and the − 429 T > C and Gly82Ser) with HT.Subject and methods.Our case–control study has 80 patients enrolled who have newly been diagnosed with HT and 80 age and sex-matched healthy female controls. Each participant underwent a thorough medical history, physical examination, and laboratory investigations, which included Genotyping of RAGE Gly82Ser, − 374 T > A and − 429 T > C using polymerase chain reaction-restriction fragment length polymorphisms (PCR–RFLP).ResultsChi-square revealed a significant association regarding the distribution of RAGE (− 374 T < C) genotypes TT and CC between patients and control (P = 0.04). Non-significant associations regarding the distribution of Gly82Ser genotypes Gly/Gly, Gly/Ser, Ser/Ser were found between patients and control (P = 0.5), and non-significant associations related to − 429 T > C gene polymorphism were revealed. In addition, patients with HT had higher MDA and lower TCA compared with controls.ConclusionThe elevated MDA and decreased TAC as an antioxidant may be one of several risk factors associated with the prevalence of HT in individuals with the − 429 T > C RAGE mutation polymorphism that is associated with an increased risk of HT in Egyptian females.

Effect of an Antioxidant Supplement Combination on Boar Sperm.

Simple SummaryBoar spermatozoa can be exposed to many harmful factors. The low antioxidant capacity of spermatozoa makes them susceptible to reactive oxygen species. The protection of spermatozoa from these harmful effects lies in the seminal plasma, which is enriched by antioxidant enzymes. This study aimed to measure the antioxidant status of boar seminal plasma, and to determine spermatozoa DNA status, and the total number of motile spermatozoa after the concurrent joint application in the boars’ diet of two commercial products with antioxidative potential.The study was conducted on a commercial pig farm located in Serbia. Thirty Duroc or Landrace breed boars were randomly selected for this study. The experimental group was fed a compound feed with added organic selenium and Oxynat 3D. The antioxidant status parameters of boar seminal plasma were evaluated using a biochemical analyzer and commercial Randox kits. The sperm chromatin structure assay (SCSA) using flow cytometry (FC) provided information about spermatozoa’s DNA status. Additionally, the total number of motile spermatozoa and spermatozoa kinematic parameters were measured using the computer-assisted sperm analysis (CASA) system. The aim of this study was to improve the parameters of semen by combining two preparations that have a potential antioxidant effect, but also to establish the level of various antioxidant enzymes in native sperm. There was no statistically significant difference in total antioxidant capacity and glutathione peroxidase activity in the seminal plasma obtained from the experimental and control groups of boars. Regarding the superoxide dismutase activity, the research results showed a difference in the control group compared to the experimental one. Moreover, spermatozoa DNA fragmentation and the total number of motile spermatozoa showed statistically significant lower and higher values, respectively, in experimental compared to the control groups. The combination of these two preparations shows significantly enhanced vital parameters of semen. To the best of our knowledge, this study is the first in which the ejaculate parameters were examined after the application of a combination of these two antioxidant supplements.

Effects of Soluble and Insoluble Non-Starch Polysaccharides on Growth Performance, Digestive Enzyme Activity, Antioxidant Capacity, and Intestinal Flora of Juvenile Genetic of Improvement of Farmed Tilapia (Oreochromis niloticus)

This study aimed to evaluate the effects of soluble and insoluble non-starch polysaccharides (NSPs) on growth performance, digestive enzyme activity, antioxidant capacity, and intestinal flora of juvenile GIFT tilapia (Oreochromis niloticus). Four isonitrogenous and isolipidic experimental diets were formulated, including the FM diet (0% NSPs, FM), INSP diet (5.8% insoluble NSPs, INSP), SNSP diet (12.2% soluble NSPs, SNSP), and NSP diet (5.8% INSP and 12.2% SNSP, NSPs). Each diet was fed to triplicate groups of 40 fish (2.14 ± 0.04 g) per repetition for 10 weeks. Results showed that dietary supplementation with different types of NSPs significantly affected the digestive enzyme activity, antioxidant capacity, and intestinal flora of juvenile GIFT tilapia. Specifically, dietary supplementation with INSP significantly increased pepsin and intestinal lipase activity, while dietary supplementation with SNSP significantly decreased intestinal lipase activity and resulted in a significant decrease in plasma catalase, glutathione peroxidase, and total antioxidant capacity activity. Meanwhile, dietary supplementation with SNSP significantly increased the concentration of urea nitrogen and high-density lipoprotein cholesterol in plasma and alanine aminotransferase activity in plasma and hepatic. Moreover, LEfSe analysis showed that dietary supplementation with SNSP significantly increased the abundance of Bacteroidales, Bacteroidia, and Cellulosilyticum, while dietary supplementation with NSPs significantly decreased the abundance of Mycobacteriaceae, Mycobacterium_neoaurum, Corynebacteriales, and Mycobacterium. These results suggest that dietary INSP is an inert ingredient with limited effect, while dietary SNSP inhabited antioxidant capacity, induced liver damage, and altered the abundance of anaerobic/aerobic bacteria in the intestine of GIFT tilapia.

Occupational risk assessment of organophosphates with an emphasis on psychological and oxidative stress factors.

Organophosphate pesticides (OPPs) are widely used all over the world in domestic and industrial settings, but these chemicals affect the nervous system, induce suicidal thoughts, depression and anxiety, and impair sleep quality. The purpose of this study was to investigate the relationship between the main toxicity mechanisms of OPPs, oxidative stress and cholinesterase inhibition, and psychological parameters in chronic exposure to OPPs. This cross-sectional study was conducted on 56 male OPPs factory workers as the worker group and 47 unexposed individuals within the same age range as the control group. Psychological factors were assessed using validated questionnaires. The activity of plasma cholinesterase and oxidative stress biomarkers, total antioxidant capacity of plasma, lipid peroxidation (LPO), and protein carbonylation were determined in blood samples by spectrophotometer. Sleep quality score in the factory workers was lower, and depression and suicidal ideation scores were higher than those in the control group. These factory workers showed 35% lower levels of plasma cholinesterase activity than did the controls. Compared to the control group, a significant impairment in oxidative stress biomarkers was also observed in the workers. Meanwhile, there was a significant relationship between the duration of employment and the level of LPO as well as a significant correlation between the quality of sleep and plasma cholinesterase in the workers. In conclusion, long-term exposure to OPPs could cause oxidative damages and neurobehavioral effects. The close monitoring of workplace exposure to organophosphates pesticides and also their respective solvents along with the reduction of working hours are of the necessities to avoid the adverse impacts of exposure to these pesticides.

MO279: Vitamin D Supplementation Attenuates Hyperandrogenemia-Associated Acute Kidney Injury in Female Sprague-Dawley Rats

Abstract BACKGROUND AND AIMS Polycystic ovary syndrome (PCOS) is closely related to the onset and development of metabolic abnormalities. However, the correlation between PCOS and kidney injury has not been clarified, and the underlying mechanism remains unknown. Vitamin D therapy has beneficial effects in women with PCOS and renal injuries. Therefore, this study was designed to explain the mechanisms responsible for kidney damages in a model of hyperandrogenic rats and the healing effects of vitamin D. METHOD Female Sprague-Dawley rats were randomly divided into five groups (n = 10): (i) control, (ii) sham, (iii) dehydroepiandrosterone (DHEA, 6 mg/100 g day-1 S.Q.), (iv) DHEA + vitamin D (1000 IU/Kg; 3 days/week), and (v) vitamin D. Plasma total testosterone, plasma creatinine (Cr), blood urea nitrogen (BUN), plasma and tissue levels of total oxidant status (TOS), total antioxidant capacity (TAC) and oxidative stress index (OSI), and histopathological changes of ovary and kidney were determined. RESULTS Plasma testosterone increased 9-fold in DHEA-treated rats compared with controls. They also had increased Cr, BUN, and severe renal tubular injury. Plasma and tissue TAC levels showed significant decreases, whereas TOS and OSI showed significant increases in the DHEA group. A significant injury in the glomerular and tubular parts of the kidney and ovarian follicular structure was observed in DHEA-administered rats. Vitamin D treatment attenuated TOS levels and significantly improved TAC levels. Kidneys and ovaries histopathological changes were significantly improved by vitamin D treatment. CONCLUSION Hyperandrogenemia causes systemic abnormalities through oxidative stress-related mechanisms, followed by obvious destruction to renal and ovarian tissues. Vitamin D supplementation attenuated these hyperandrogenemia-associated acute kidney injuries.

Evaluation of antioxidants, nitrosative, and oxidative stress before & after acute brucellosis treatment

BackgroundIn vivo studies of antioxidants, nitrosative, and oxidative processes in Brucella infection have not been comprehensive. This research looked at these critical concerns before and after treating individuals with acute brucellosis. MethodsA total of 50 individuals with acute brucellosis were studied before and after treatment in Babol, Iran. Superoxide dismutase (SOD) activity, plasma total antioxidant capacity (TAC), catalase (CAT) activity, protein carbonyl, oxidative stress markers like malondialdehyde (MDA), and nitrosative stress markers such as nitrite oxide metabolites (NO3, NO2) were all assessed. ResultsThe individuals were 44.15 ± 13.5 years old on average. Before therapy, protein carbonyl and plasma MDA were 0.887 ± 0.12 and 0.725 ± 0.33, respectively, and after treatment, they were 0.697 ± 0.17 and 0.467 ± 0.48 (p < 0.05). After treatment, the average TAC level was greater (870.1 ± 88.64 mol/l vs. 670.13 ± 133.96 mol/l) (p < 0.001). Before and after therapy, CAT activity was significantly different (8.53 ± 2.70 vs. 6.94 ± 2.84, p < 0.001). In addition, SOD activity was 41.79 ± 9.79 before treatment and 28.95 ± 8.11 after treatment (p < 0.001). The difference in plasma nitrite oxide levels before and after therapy was substantial (50.89 ± 17.74 vs. 28.68 ± 15.97, p < 0.001). ConclusionThe findings revealed that in acute brucellosis, nitrosative and oxidative stress, as well as antioxidant depletion, were all elevated. The addition of appropriate natural antioxidants to therapy regimens may improve their effectiveness.