An experiment was carried out to study the transport process of nitrogen (N) assimilation from tea roots by monitoring the dynamic composition of N compounds in xylem sap after 15N-NO 3 and 15N-NH 4 were fed to the root of tea plants ( Camellia sinensis L.). Results showed that the main amino acids were glutamine, theanine, arginine, asparic acid and glutamic acid, which accounted for 49%, 17%, 8%, 7%, and 4%, respectively, of the total amino acids in the xylem sap. After the tea plants were fed with 15N-NO 3 and 15N-NH 4 for 48 h, the amount of total amino acids in xylem sap significantly increased and those fed with 15N-NH 4 had higher increment than those with 15N-NO 3. Two hours after 15N- NO 3 and 15N-NH 4 were fed, 15N abundance in glutamine, asparagine, glutamic acid, alanine, and arginine were detected and increased quickly over time. This indicated that it took less than 2 h for NO 3-N and NH 4-N to be absorbed by tea roots, incorporated into the above amino acids and transported to the xylem sap. Rapid increase in 15N-NO 3 in the xylem sap of tea plants fed with 15N-NO 3 indicated that nitrate could be directly transported to the xylem sap. Glutamine, theanine, and alanine were the main amino acids transported in xylem sap of tea plants fed with both 15N-NO 3 and 15N-NH 4.