Total Adipose Tissue Weight Research Articles

Polysaccharide-Rich Red Algae (Gelidium amansii) Hot-Water Extracts Alleviate Abnormal Hepatic Lipid Metabolism without Suppression of Glucose Intolerance in a Streptozotocin/Nicotinamide-Induced Diabetic Rat Model.

This study was designed to investigate the effects of polysaccharide-rich red algae (Gelidium amansii) hot-water extracts (GHE) on lipid and glucose metabolism in rats with streptozotocin (STZ)/nicotinamide (NA)-induced diabetes. Rats were divided into three groups: NC—normal control group), DM—diabetic group, and DG—diabetic group supplemented with GHE (5%). The experimental diet and drinking water were available ad libitum for 10 weeks. After the 10-week feeding duration, the body weight, liver weight, total adipose tissue weight, and hepatic TBARS and cholesterol levels were significantly increased, and hepatic glycogen content and adipose lipolysis rate were significantly decreased in the DM group, which could be effectively reversed by supplementation of GHE. However, GHE supplementation could not improve the glucose intolerance in DM rats. It was interesting to note that GHE supplementation could decrease the liver glucose-6-phosphotase activity, which was increased in DM rats. Taken together, these results suggested that GHE feeding may ameliorate abnormal hepatic lipid metabolism, but not glucose intolerance, in diabetic rats induced by STZ/NA.

Blueberry and cranberry anthocyanin extracts reduce bodyweight and modulate gut microbiota in C57BL/6J mice fed with a high-fat diet.

Blueberry and cranberry are rich in anthocyanins. The present study was to investigate the effects of anthocyanin extracts from blueberry and cranberry on body weight and gut microbiota. C57BL/6J Mice were divided into six groups (n = 9 each) fed one of six diets namely low-fat diet (LFD), high-fat diet (HFD), HFD with the addition of 1% blueberry extract (BL), 2% blueberry extract (BH), 1% cranberry extract (CL), and 2% cranberry extract (CH), respectively. Feeding BL and BH diets significantly decreased body weight gain by 20-23%, total adipose tissue weight by 18-20%, and total liver lipids by 16-18% compared with feeding HFD. Feeding CH diet but not CL diet reduced the body weight by 27%, accompanied by a significant reduction of total plasma cholesterol by 25% and tumor necrosis factor alpha(TNF-α) by 38%. The metagenomic analysis showed that the supplementation of blueberry and cranberry anthocyanin extracts reduced plasma lipopolysaccharide concentration, accompanied by a reduction in the relative abundance of Rikenella and Rikenellaceae. Dietary supplementation of berry anthocyanin extracts promoted the growth of Lachnoclostridium, Roseburia, and Clostridium_innocuum_group in genus level, leading to a greater production of fecal short-chain fatty acids (SCFA). It was concluded that both berry anthocyanins could manage the body weight and favorably modulate the gut microbiota at least in mice.

Stachys sieboldii Miq. Root Attenuates Weight Gain and Dyslipidemia in Rats on a High-Fat and High-Cholesterol Diet.

This study aimed at investigating the anti-obesity and anti-dyslipidemic effects of Stachys sieboldii Miq. root (SS) powder in rats following a high-fat and high-cholesterol (HFC) diet for 6 weeks. Thirty-two Sprague–Dawley rats were fed one of the following diets: a regular diet (RD), HFC, HFC supplemented with 3% SS (HFC + 3SS) or HFC supplemented with 5% SS (HFC + 5SS). Following an HFC diet increased body weight (BW) gain (p < 0.001) and the food efficiency ratio (FER; p < 0.001); however, SS consumption gradually prevented the HFC-induced BW gain (p < 0.001) and increase in FER (p < 0.01). The HFC diet resulted in increased liver size (p < 0.001) and total adipose tissue weight (p < 0.001), whereas the SS supplementation decreased hepatomegaly (p < 0.05) and body fat mass (p < 0.001). SS consumption prevented the increased activities of serum alanine aminotransferase (ALT; p < 0.001), aspartate aminotransferase (AST; p < 0.001), alkaline phosphatase (ALP; p < 0.01 in HFC + 5SS) and lactate dehydrogenase (LDH; p < 0.001 in HFC + 5SS) induced by the HFC diet (p < 0.001). The SS supplementation improved lipid profiles in the circulation by lowering triglyceride (TG; p < 0.01), total cholesterol (TC; p < 0.001) and non-HDL cholesterol (non-HDL-C; p < 0.001) levels, as well as the atherogenic index (p < 0.01) and cardiac risk factor (p < 0.01). The lipid distribution in the liver (p < 0.05) and white adipose tissues (WAT; p < 0.001) of the HFC + SS diet-consuming rats was remarkably lower than that of the HFC diet-consuming rats. The average size of the epididymal adipose tissue (p < 0.001) was significantly lower in the HFC + SS diet-fed rats than in the HFC diet-fed rats. The fecal lipid (>3% SS; p < 0.001) and cholesterol (5% SS; p < 0.001) efflux levels were significantly elevated by the SS supplementation compared to those measured in the RD or HFC diet-fed groups. In addition, the hepatic lipid and cholesterol metabolism-related gene expressions were affected by SS consumption, as the hepatic anabolic gene expression (Acc; p < 0.001, Fas; p < 0.001 and G6pdh; p < 0.01) was significantly attenuated. The HFC + 5SS diet-fed rats exhibited elevated hepatic Cyp7a1 (p < 0.001), Hmgcr (p < 0.001) and Ldlr (p < 0.001) mRNA expression levels compared to the HFC diet-fed rats. These results suggest that SS may possess anti-adipogenic and lipid-lowering effects by enhancing lipid and cholesterol efflux in mammals.

Effects of resveratrol and its analogue pterostilbene, on NOV/CCN3 adipokine in adipose tissue from rats fed a high-fat high-sucrose diet.

Nephroblastoma overexpressed protein, also called NOV/CCN3, is an adipokine which is present in various tissues and recently linked to obesity. The objective of the study was to determine the effect of resveratrol and pterostilbene on NOV/CCN3 in adipose tissue from rats fed an obesogenic diet. Thirty-six male Wistar rats were split into four groups (n = 9): fed a standard diet (CC), high-fat high-sucrose (HFS) diet supplemented with resveratrol (RSV; 30mg/kg/day) or with pterostilbene (PT; 30mg/kg/day), or without phenolic supplementation (HFS). Rats were sacrificed after 6weeks of treatment, and adipose tissue (white and brown) from different anatomical locations were dissected. Then, Nov/ccn3 gene and protein expression and the adipogenic genes, Ucp-1 and Pgc-1a, expressions were studied. Increased weight of white adipose tissues was found in rats fed the HFS diet. Whereas resveratrol-treated rats showed reduced internal and total adipose tissue weights, pterostilbene-treated rats showed reduced subcutaneous, internal and total adipose depots. Nov/ccn3 gene expression decreased in epididymal and interscapular brown depot in rats fed HFS diet when compared with the control group. Regarding the phenolic compounds, resveratrol prompted a Nov/ccn3 gene expression increase in epididymal fat tissue, whereas pterostilbene reduced its protein expression compared with the obese group. However, these phenolic compounds did not affect NOV/CCN3 expression in brown depot. NOV/CCN3 seems to be involved in weight changes in epididymal adipose tissue under obesogenic feeding, but not in subcutaneous, acting as a protective mechanism counteracting the fattening effect of the diet. To our knowledge, this is the first study analyzing whether NOV/CCN3 is involved in the anti-obesity effect of resveratrol and pterostilbene. Our results suggest that this is not the case.

The effects of Brassica juncea L. leaf extract on obesity and lipid profiles of rats fed a high-fat/high-cholesterol diet.

BACKGROUND/OBJECTIVESObesity is a global health problem of significant importance which increases mortality. In place of anti-obesity drugs, natural products are being developed as alternative therapeutic materials. In this study, we investigated the effect of Brassica juncea L. leaf extract (BLE) on fat deposition and lipid profiles in high-fat, high-cholesterol diet (HFC)-induced obese rats.MATERIALS/METHODSMale Sprague-Dawley rats were divided into four groups (n = 8 per group) according to diet: normal diet group (ND), high-fat/high-cholesterol diet group (HFC), HFC with 3% BLE diet group (HFC-A1), and HFC with 5% BLE diet group (HFC-A2). Each group was fed for 6 weeks. Rat body and adipose tissue weights, serum biochemical parameters, and tissue lipid contents were determined. The expression levels of mRNA and proteins involved in lipid and cholesterol metabolism were determined by reverse transcription polymerase chain reaction and western blot analysis, respectively.RESULTSThe HFC-A2 group showed significantly lower body weight gain and food efficiency ratio than the HFC group. BLE supplementation caused mesenteric, epididymal, and total adipose tissue weights to decrease. The serum levels of triglyceride, total cholesterol, and low-density lipoprotein cholesterol were significantly reduced, and high-density lipoprotein cholesterol was significantly increased in rats fed BLE. These results were related to lower glucose-6-phosphate dehydrogenase, acetyl-coA carboxylase, and fatty acid synthase mRNA expression, and to higher expression of the cholesterol 7α-hydroxylase and low density lipoprotein-receptor, as well as increased protein levels of peroxisome proliferator-activated receptor α. Histological analysis of the liver revealed decreased lipid droplets in HFC rats treated with BLE.CONCLUSIONSSupplementation of HFC with 3% or 5% BLE inhibited body fat accumulation, improved lipid profiles, and modulated lipogenesis- and cholesterol metabolism-related gene and protein expression.

Antiobesity Effects of Sansa (Crataegi fructus) on 3T3-L1 Cells and on High-Fat-High-Cholesterol Diet-Induced Obese Rats.

This study was performed to investigate the effects of Crataegi fructus ethanol extracts (CFEEs) on the differentiation of 3T3-L1 cells, and to evaluate the effects of C. fructus powder (CFP) on lipid metabolism and its antiobesity effect in rats fed a high-fat and high-cholesterol (HFC) diet. Both in vitro and in vivo studies were performed for physiological activity and antiobesity effects on the serum, liver, and adipose tissues in obesity-induced rats. CFEEs showed significant inhibitory action on differentiation and triglyceride (TG) accumulation in 3T3-L1 mature cells in a dose-dependent manner. Subcutaneous, mesenteric, epididymal, and total adipose tissue weights of HFC diet group were heavier than those of normal diet (N) group, whereas those of groups fed CFP were significantly decreased. Levels of serum TGs, total cholesterol (TC), and low-density lipoprotein cholesterol were significantly decreased in the CFP groups than in the HFC group, whereas the serum high-density lipoprotein cholesterol level decreased in the HFC group and markedly increased in the CFP groups. TC and TG levels in the liver and adipose tissues were significantly lower in CFP groups than in the HFC groups. In addition, feeding with CFP significantly reduced the occurrence of fatty liver deposits and steatosis, and inhibited an HFC diet-induced increase in adipocyte size. These results suggest that C. fructus may improve lipid metabolism in the serum, liver, and adipose tissue, and may potentially reduce lipid storage.

Effect of dietary bioactive compounds and biopolymer encapsulated lipids on metabolism of lipids in high fat diet‐fed mice

We determined the effects of dietary bioactive components and biopolymer encapsulation on lipids’ metabolism in obese mice. Six random groups of 60 obese mice were fed the following diets for 95 days: Basal diet; cellulose encapsulated lipids; pectin and chitosan encapsulated lipids (EPC); Camellia sinensis and Coix lacryma enhanced diet; C. sinensis and C. lacryma diet plus pectin and chitosan encapsulated lipids; hydroxycitric acid, caffeine, and vitamin C enhanced diet plus pectin and chitosan encapsulated lipids (HCVEPC). The HCVEPC group usually presented lower body and total adipose tissue weight compared to other diet groups. Intestine length was not significantly different among all dietary groups. Serum leptin, triglycerides, and cholesterol were significantly lower in EPC and HCVEPC compared to other groups. However, serum glucose was not significantly different among groups. Our results indicate that bioactive compounds and biopolymers’ encapsulation act synergistically in the reduction of body and adipose tissue weight.Practical applications: Based on the results described above, it is suggested that bioactive components and biopolymer encapsulation with some natural products has synergistically in the reduction of body and adipose tissue weight on in vivo models. In addition, through this results suggest that can be used information and utilization of lipids metabolism, this research is to highlight the potential of selected natural products as a source of functional Substances. Therefore, lipids encapsulated with dietary biopolymers and bioactive compounds should be considered as a prospective functional food and pharmaceuticals application.We investigated effects of dietary bioactive components and biopolymer encapsulation on lipids’ metabolism in obese mice. Bio‐active components could enhance the effect of body and adipose tissue weight reduction in obese mice models. Dietary bio‐active compounds together with biopolymer encapsulated lipids showed a great reduction in serum triglycerides and cholesterol.

Plasma amino acid and metabolite signatures tracking diabetes progression in the UCD-T2DM rat model.

Elevations of plasma concentrations of branched-chain amino acids (BCAAs) are observed in human insulin resistance and type 2 diabetes mellitus (T2DM); however, there has been some controversy with respect to the passive or causative nature of the BCAA phenotype. Using untargeted metabolomics, plasma BCAA and other metabolites were assessed in lean control Sprague-Dawley rats (LC) and temporally during diabetes development in the UCD-T2DM rat model, i.e., prediabetic (PD) and 2 wk (D2W), 3 mo (D3M), and 6 mo (D6M) post-onset of diabetes. Plasma leucine, isoleucine, and valine concentrations were elevated only in D6M rats compared with D2W rats (by 28, 29, and 30%, respectively). This was in contrast to decreased plasma concentrations of several other amino acids in D3M and/or D6M relative to LC rats (Ala, Arg, Glu, Gln, Met, Ser, Thr, and Trp). BCAAs were positively correlated with fasting glucose and negatively correlated with plasma insulin, total body weight, total adipose tissue weight, and gastrocnemius muscle weight in the D3M and D6M groups. Multivariate analysis revealed that D3M and D6M UCD-T2DM rats had lower concentrations of amino acids, amino acid derivatives, 1,5-anhydroglucitol, and conduritol-β-opoxide and higher concentrations of uronic acids, pantothenic acids, aconitate, benzoic acid, lactate, and monopalmitin-2-glyceride relative to PD and D2W UCD-T2DM rats. The UCD-T2DM rat does not display elevated plasma BCAA concentrations until 6 mo post-onset of diabetes. With the acknowledgement that this is a rodent model of T2DM, the results indicate that elevated plasma BCAA concentrations are not necessary or sufficient to elicit an insulin resistance or T2DM onset.

Rice koji reduced body weight gain, fat accumulation, and blood glucose level in high-fat diet-induced obese mice.

Rice koji is considered a readily accessible functional food that may have health-promoting effects. We investigated whether white, yellow, and red koji have the anti-obesity effect in C57BL/6J mice fed a high-fat diet (HFD), which is a model for obesity. Mice were fed HFD containing 10% (w/w) of rice koji powder or steamed rice for 4 weeks. Weight gain, epididymal white adipose tissue, and total adipose tissue weight were significantly lower in all rice koji groups than in the HFD-rice group after 4 weeks. Feed efficiency was significantly reduced in the yellow koji group. Blood glucose levels were significantly lower in the white and red koji groups with HOMA-R and leptin levels being reduced in the white koji group. White and red koji increased glucose uptake and GLUT4 protein expression in L6 myotube cells. These results showed that all rice koji have the anti-obesity or anti-diabetes effects although the mechanisms may differ depending on the type of rice koji consumed.

Effects of Dietary Conjugated Linoleic Acid and Biopolymer Encapsulation on Lipid Metabolism in Mice

Forty mice were randomly divided into four groups on the basis of the diet to be fed as follows: 5% (low) fat diet (T1: LF); 20% (high) fat diet (T2: HF); 20% fat containing 1% conjugated linoleic acid (CLA) (T3: HFC); and 20% fat containing 1% CLA with 0.5% biopolymers (T4: HFCB). The high-fat with CLA diet groups (HFC and HFCB) and the low-fat diet group (LF) tended to have lower body weights and total adipose tissue weights than those of the high-fat diet group (HF). Serum leptin and triglyceride were significantly lower in the high fat with CLA-fed groups (HFC and HFCB) and the low-fat diet group (LF) than those in the high-fat diet group (HF). It is noteworthy that the high-fat with CLA and biopolymers group (HFCB) showed the lowest serum triglyceride and cholesterol concentrations. In the high-fat-fed group (HF), voluntary travel distance as a measure of physical activity decreased after three weeks of feeding. However, the CLA-fed groups showed increased physical activity. The groups fed high-fat diets supplemented with CLA alone and with CLA and biopolymers had higher viscosity of small intestinal contents than that in the low- and high-fat dietary groups.

Antiobesity and antidiabetic effects of Jerusalem artichoke and purple sweet potato in the diet‐induced obese rats

The study was conducted to investigate the antiobesity effect of Jerusalem artichoke and purple sweet potato supplementation in diet‐induced obese rats. High fat diet was given for 4 weeks and experimental diets for 5 weeks. Thirty‐two 3‐week‐old male Sprague‐Dawley rats were assigned to four groups (n=8/group); normal diet(ND), high‐fat diet(HFD), high fat diet with 10% Jerusalem artichoke(JA), or high fat diet with 10% purple sweet potato(SP). Final body weight, total and average body weight gain of JA and Sp groups were significantly lower or similar to ND group. Total adipose tissue weight was the lowest in ND and JA group, followed by SP and HFD group(p&lt;0.05), and similar tendency was observed in epididymal, perirenal, and visceral white adipose tissue (p&lt;0.05) without difference in interscapular brown adipose tissue. Adipocyte size of epdidymis in JA and SP groups were lower than HFD group and similar to ND group(p&lt;0.05), although the subcutaneous adipose tissue did not differ among the groups. Serum insulin level did not differ, but serum glucose level and HOMA‐IR were lower in JA and SP groups than those of HFD group(p&lt;0.05). Taken together, the supplementation of JA and SP exerted the antiobesity and antidiabetic effects in diet‐induced obese rats and may be a good source for preventing obesity and diabetes. Further research should be conducted to elucidate the mechanism involved in those effects.

Hypertension and abnormal fat distribution but not insulin resistance in mice with P465L PPARγ

Peroxisome proliferator–activated receptor γ (PPARγ), the molecular target of a class of insulin sensitizers, regulates adipocyte differentiation and lipid metabolism. A dominant negative P467L mutation in the ligand-binding domain of PPARγ in humans is associated with severe insulin resistance and hypertension. Homozygous mice with the equivalent P465L mutation die in utero. Heterozygous mice grow normally and have normal total adipose tissue weight. However, they have reduced interscapular brown adipose tissue and intra-abdominal fat mass, and increased extra-abdominal subcutaneous fat, compared with wild-type mice. They have normal plasma glucose levels and insulin sensitivity, and increased glucose tolerance. However, during high-fat feeding, their plasma insulin levels are mildly elevated in association with a significant increase in pancreatic islet mass. They are hypertensive, and expression of the angiotensinogen gene is increased in their subcutaneous adipose tissues. The effects of P465L on blood pressure, fat distribution, and insulin sensitivity are the same in both male and female mice regardless of diet and age. Thus the P465L mutation alone is sufficient to cause abnormal fat distribution and hypertension but not insulin resistance in mice. These results provide genetic evidence for a critical role for PPARγ in blood pressure regulation that is not dependent on altered insulin sensitivity.

Hypertension and abnormal fat distribution but not insulin resistance in mice with P465L PPARgamma.

Peroxisome proliferator-activated receptor gamma (PPARgamma), the molecular target of a class of insulin sensitizers, regulates adipocyte differentiation and lipid metabolism. A dominant negative P467L mutation in the ligand-binding domain of PPARgamma in humans is associated with severe insulin resistance and hypertension. Homozygous mice with the equivalent P465L mutation die in utero. Heterozygous mice grow normally and have normal total adipose tissue weight. However, they have reduced interscapular brown adipose tissue and intra-abdominal fat mass, and increased extra-abdominal subcutaneous fat, compared with wild-type mice. They have normal plasma glucose levels and insulin sensitivity, and increased glucose tolerance. However, during high-fat feeding, their plasma insulin levels are mildly elevated in association with a significant increase in pancreatic islet mass. They are hypertensive, and expression of the angiotensinogen gene is increased in their subcutaneous adipose tissues. The effects of P465L on blood pressure, fat distribution, and insulin sensitivity are the same in both male and female mice regardless of diet and age. Thus the P465L mutation alone is sufficient to cause abnormal fat distribution and hypertension but not insulin resistance in mice. These results provide genetic evidence for a critical role for PPARgamma in blood pressure regulation that is not dependent on altered insulin sensitivity.

Relationships between visceral, trunk and whole-body adipose tissue weights by cadaver dissection

Background: Despite the important association of central adiposity and cardiovascular and other risk factors, there are only three reported values for directly weighed visceral adipose tissue (AT). All other reported values are based on medical imaging techniques.Objective: The study aimed to investigate the relationships between visceral, trunk and total AT weights in older men and women.Methods: Data was obtained from the combination of two studies involving the complete dissection of 15 male and 16 female cadavers (age range 55–94 years) and allowed for compartmentation into skin, AT, muscle, bone and a residual component, divided over six body segments: head, trunk, legs and arms. Visceral AT was separated from trunk subcutaneous AT. All tissues were weighed.Results: Visceral AT weights ranged from 0.3 to 5.8 kg. Mean values were 3.00 ± 1.52 kg (mean ± SE) for the men and 3.24 ± 1.67 kg for the women. These were not significantly different ( p = 0.68), but visceral AT weight, expressed as a percentage of total body AT weight was significantly greater ( p = 0.02) in the men (16.8 ± 5.4%) than in the women (12.9 ± 3.5%). Correlations between visceral AT weight and the weight of subcutaneous AT of the trunk were highly significant (men, r = 0.70, women, r = 0.81, p < 0.005), with similar slopes for the two sexes. The correlation coefficients of visceral with total body AT weights were even greater (men, r = 0.83 and women, r = 0.96, p < 0.0001).Conclusions: In this sample of older Belgians, visceral AT is strongly related to total body adiposity, corresponding to an increment of about 200 g of visceral AT for every kilogram of total AT in men and about 180 g in women. Because of this relationship, techniques such as skinfold calipers and ultrasound for assessing whole body fatness from measurement of only the subcutaneous layer are thus able to account for visceral adiposity.

Relationship of anthropometric indices of body fat to cardiovascular risk in Japanese women.

The relationship between body fat indices and cardiovascular risk was analyzed in 97 Japanese women aged 36-72 years. The variables used were anthropometric measures of body fatness, blood lipids and blood pressure. Total adipose tissue weight (TATW) was estimated from bioelectrical impedance measurements. Subcutaneous adipose tissue weight (SATW) was calculated by measuring subcutaneous fat thickness at 14 sites using a skinfold caliper. The indices of body fat distribution were the ratios of waist to hip circumference (WHR) and abdominal to hip circumference (AHR). Blood pressure and serum lipid levels were determined in all subjects after an overnight fast. The correlations among the various body fat indices were high, and all were strongly correlated with WHR and AHR; only % SATW and percentage internal adipose tissue weight (%IATW) were not correlated with WHR and AHR. Significant positive associations were found between WHR, triglycerides and systolic blood pressure (SBP). Negative associations were found between WHR and HDL-cholesterol (HDL-ch.) and the ratio of HDL-ch. to total cholesterol (HDL-ch./T-ch.). On the other hand, significant positive associations were found between AHR, T-ch., LDL-cholesterol, free fatty acid and SBP. A negative association was found between AHR and HDL-ch./T-ch.. Partial correlations were used to determine further associations between cardiovascular risk factors and fat distribution indices. SBP was associated with WHR or AHR after removing the effects of age. Similarly, HDL-ch./T-ch. was associated with WHR or AHR after removing the effects of age and degree of obesity (BMI). These results suggest that the WHR and AHR are equally valid estimates of body fat distribution with respect to their relationship with cardiovascular risk. These relationships occurred independently of age or BMI in Japanese women. These findings emphasize the importance of intra-abdominal obesity as a metabolic risk factor for cardiovascular disease associated with a high WHR and AHR.

Age-Related Changes in Body Fat Distribution in Middle-Aged and Elderly Japanese.

Total body fat mass was estimated in a group of 65 healthy male and 39 healthy female Japanese aged 40-77 using the D2O dilution method, and by measurement of skinfold thickness at 14 sites. The weights of skin, and subcutaneous and internal adipose tissue were calculated. The women had a higher total adipose tissue weight than the men (p<0.01). Absolute subcutaneous adipose tissue weight in women was about double that in men. However, absolute internal adipose tissue weight and internal adipose tissue weight as a percentage of body weight showed no significant sex difference. The relationship of total and subcutaneous adipose tissue weight with age showed a correlation of r=-0.283 (p<0.05) and r=-0.367 (p<0.01), respectively, for men and r=-0.329 (p< 0.05) and r=-0.428 (p<0.01), respectively, for women. The correlation of internal adipose tissue weight with age was not significant, but the internal adipose tissue weight/total adipose tissue weight ratio increased steadily with age in both sexes.