Equilibrium configurations of trans- and cis- conformers of the HSSSH molecule (trisulfane), as well as the vibrational spectra of both conformers, were calculated at the MP2/Aug-cc-pVTZ, level of theory in the harmonic and anharmonic approximations. A more accurate analysis of torsional vibrations of thiol groups was performed by constructing 2D surfaces of a) potential energy, b) kinetic coefficients, and c) dipole moment components as functions of torsional coordinates of S-H groups. The values of b) and c) were calculated at the MP2/Aug-cc-pVTZ level of theory while the values of a) were calculated at the B3LYP/Aug-cc-pVTZ, MP2/cc-pVTZ, MP2/Aug-cc-pVTZ, MP2/cc-pVQZ, MP2/CBS(T,Q) and CCSD(T)/Aug-cc-pVTZ levels of theory. The energies of the stationary torsional states of the trisulfane molecule conformers were determined by numerically solving the vibrational Schrödinger equation of restricted dimensionality. The frequencies of torsional vibrations of S-H groups, as well as the splittings of torsional energy levels in trans- and cis-conformers due to tunneling between equivalent configurations of the molecule, were determined. The calculated values of tunneling frequencies in the ground torsional state of HSSSH and DSSSD trans-conformers (2.3 * 10−22 and 7.5 * 10−27 cm−1, respectively) are of particular interest due to the fact that trisulfane is considered to be one of the best candidates for establishing the effect of violation of parity conservation law in chiral molecules.