This study aimed to analyze spatio-temporal changes in habitat quality in Chang-Zhu-Tan Urban Agglomeration during the 2000–2020 period and explore its topographic gradient effects. Using land use data from this period, the InVEST model was employed to assess the spatio-temporal variations in habitat quality. The bivariate spatial autocorrelation model was used to analyze the spatial correlation characteristics between habitat quality and various topographical factors. Additionally, terrain factor analysis was utilized to study the terrain gradient effects on habitat quality in the study area. The results reveal that: (1) The primary land use changes in the study area from 2000 to 2020 predominantly involve substantial arable land and forest conversions into urban development. (2) The average habitat quality indices for 2000, 2010, and 2020 in the Chang-Zhu-Tan Urban Agglomeration stand at 0.651, 0.622, and 0.606, respectively, indicating a consistent declining trend in habitat quality. The distribution of habitat quality grades demonstrates a spatial pattern of “lower in the central surroundings, higher in the surroundings.” (3) The Chang-Zhu-Tan Urban Agglomeration shows significant positive correlations between habitat quality and topographical gradients. Spatial aggregation tendencies between habitat quality and topographical gradients primarily exhibit “high-high” and “low-low” clustering. (4) The habitat quality of the Chang-Zhu-Tan urban agglomeration exhibits a significant topographic gradient effect, primarily characterized by an increase in habitat quality with the rise of the topographic gradient. The study outcomes contribute to unveiling the spatiotemporal variations in habitat quality within the Chang-Zhu-Tan Urban Agglomeration. Moreover, leveraging different habitat types’ distinctive terrain distribution characteristics, it proposes targeted habitat conservation measures, thereby offering theoretical support for biodiversity conservation and territorial spatial planning in the study area.
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