Within the context of the topcolor-assisted technicolor (TC2) model we investigate the associated production of the neutral top-higgs ht0 with a pair of top quarks, i.e. pp → tt̄ht0 at the Large Hadron Collider (LHC), which proceeds through the partonic processes qq̄ → tt̄ht0 and gg → tt̄ht0. We calculate the production rate and show the distributions of the transverse momenta of ht0. It is found that the cross section of pp → tt̄ht0 can reach a few hundred fb in the most part of the allowed parameter space and even a few tens of pb for the light top-higgs. Due to the large cross section of tt̄ht0 production and the top-charm decay mode of ht0 for mht0 < 2mt, the process pp → tt̄ht0 can play an important role in the probe of neutral top-higgs and the test of the TC2 model.