Conservation Tool Research Articles

Shelter Capacity of Artificial Reefs for Sea Cucumber Apostichopus japonicas Is Influenced by Water Flow and Food Resources in Laboratory Experiments

Artificial reefs have been advocated and implemented as management tools for aquaculture, species conservation and habitat replacement. However, the shelter capacity of artificial reefs and its influencing factors are still not well understood. In this study, we identified factors that may limit the shelter capacity of artificial reefs for sea cucumber (Apostichopus japonicas) with a laboratory experiment. We investigated how water flow and food availability affect the shelter capacity and conducted shelter utilization experiments to determine whether sea cucumber sheltering behavior was density dependent. The results revealed that shelter capacity of artificial reefs in high velocity is significantly higher than that of artificial reefs in low velocity or no velocity. The artificial reefs that were provided food had significantly higher shelter capacity than those that did not have food. The densities did not affect the sheltering capacity of artificial reefs in the laboratory experiment. There was a logarithmic relationship between the shelter capacity and shelter availability assessed by the exposed surface area of the artificial reefs. In conclusion, abundant food resources and high water flow may have a positive effect on the shelter capacity of artificial reefs.

ARCHITECTURAL PHOTOGRAMMETRY APPLIED TO THE CATALOGING OF HISTORICAL HERITAGE. A CASE STUDY OF A BUNKER IN LA LÍNEA DE LA CONCEPCIÓN, SPAIN

<p>Photogrammetry is an emerging tool in architecture, conservation, cataloging, and maintenance of heritage, offering a precise and efficient methodology for generating three-dimensional digital models. This work presents a comparative study of photogrammetric methods, focused on a lowcost software application, with the aim of evaluating its effectiveness in capturing and faithfully representing architectural and cultural elements, such as the bunkers located in La Línea de la Concepción (Spain), where the seismic hazard is not neglectable. The results reveal that the method choice depends on the object being digitized. Some programs are more suitable for capturing fine details, while others excel in reconstructing more complex structures.</p>

Artificial refuges did not increase small mammal abundance after fire

AbstractThe interacting threats of invasive predators and altered fire regimes are key conservation issues for many native species globally. Artificial refuges have been proposed as a potential conservation tool to provide prey species with protection from invasive predators after fire, but we do not yet know whether they improve animal survival. To address this knowledge gap, we experimentally tested how small mammal abundance and species richness were influenced by the provision of artificial refuges after prescribed burns. We surveyed small mammals across five unburnt sites, seven burnt sites with artificial refuges, and eight burnt control sites following two prescribed fires in southeastern Australia. There were negative and neutral responses of small mammals to the burns, and relative abundance was positively correlated with structurally complex vegetation. Artificial refuges had no impact on abundance or species richness, irrespective of burn coverage. These findings suggest that this artificial refuge design may not be an effective tool for improving small mammal population persistence postfire, and as such we should not yet scale up their application. However, given the inherent context‐dependency of field experiments involving fire, which include difficult‐to‐control variables such as fire severity, predator activity, and population dynamics, we encourage researchers to undertake further experiments with artificial refuges in fire‐affected areas, including after severe wildfires when less vegetation cover remains. Such studies will help to build our understanding of their utility as a conservation tool across different ecosystems and fire types.

Beyond the surface: Seabirds and plastics as indicators in a large, remote marine protected area

Marine protected areas (MPAs) are an important conservation tool for species and habitats; however, they are not a panacea solution. For example, MPAs provide little protection from plastic pollution which travels vast distances on ocean currents. Here we document exposure of juvenile Christmas Shearwaters (Puffinus nativitatis) to plastics on uninhabited Ducie Atoll in the remote South Pacific. Despite being surrounded by the very large Pitcairn Islands MPA, most birds (68.7 %; n = 16) contained 3.8 ± 4.1 pieces of ingested plastic. Unexpectedly, the number, mass and frequency of occurrence of plastic in two age classes (young downy chicks and fledglings) was similar. While the reason for this is unknown, it may suggest birds do not acquire new plastic items, or are able to rid themselves of plastics, beyond a certain age. We discuss the potential health consequences of plastic ingestion in Christmas Shearwaters and call for further research of this poorly studied species.

The Contribution of Genetic and Genomic Tools in Diversity Conservation: The Case of Endemic Plants of Greece

The conservation of endemic plant species has come into the global spotlight, not only because of their contribution to biodiversity but also their vulnerability and high extinction risk due to environmental and anthropogenic threats. Based on these developments, it is now essential to monitor and protect these species by applying integrated conservation strategies, especially in view of climate change, which is one of the most severe threats to plants. Genetic and genomic tools provide new potential in assessing and quantifying genetic diversity and thus can be utilized to devise conservation strategies and contribute to biodiversity conservation efforts. Greece comprises a plant biodiversity hotspot in the Mediterranean Basin with a wide variety of rare, threatened, and endemic plant taxa. In this review, we examine several cases where a broad spectrum of genetic tools has been utilized so far in the diversity assessment and conservation management of Greek Endemic Plants (GEPs). Following an extensive database search, we have identified and included in our final data collection 19 studies concerning 32 GEPs for which molecular markers have been used for the determination of population genetic structure and diversity assessment, while at the same time, the research outcomes have been taken into consideration for conservation management. The limited application of genetic and genomic tools in GEP management is demonstrated, while the significance of implementing a comprehensive conservation strategy that will integrate genetic analyses and the data derived therein is also highlighted.

A Comprehensive Review of Studies Focusing on the Intersection of Urban Flooding and Historic Urban Landscapes

Abstract Due to evolving climate patterns and the deterioration of infrastructure in historic cities, these urban locales confront escalating flood-related risks. A profusion of academic discourse has separately explored the subjects of urban flooding and historic urban landscapes, but an integrative, systematic review of both areas concurrently remains scarce. This review presents a holistic perspective of the convergence between urban flooding and historic urban landscapes, meticulously evaluating 101 scholarly articles sourced from the Web of Science database spanning from 2006 to 2022. The disciplinary classification encompasses 24 categories. A careful examination of the methodologies employed in these articles reveals flood vulnerability mapping as a prevalent tool in urban heritage conservation, while nature-based solutions emerge as potent strategies for mitigating urban flooding challenges. The insights derived from this review shed light on the present state of affairs regarding urban flooding risks in historic cities, and provide a wealth of information beneficial to decision-makers and practitioners engaged in urban stormwater management.

Public perceptions of marine protected areas: an Italian study

Marine Protected Areas (MPAs) have been gaining increasing importance in modern conditions, characterised by environmental challenges like climate change, loss of biodiversity, pollution and overexploitation of marine resources. MPAs represent an effective tool for marine conservation and the current policies aim to increase their number to protect 30% of our ocean by 2030. To obtain the achievements derived from MPAs, public engagement is paramount and requires an understanding of people’s perceptions of MPAs. Italy has a relevant coastline affected by extreme anthropic pressures linked to tourism, fisheries and other industries, with several MPAs established over the years to mitigate these pressures. Through an online questionnaire distributed in 2014 and 2021 via snowball sampling, this study investigated public perceptions of MPAs in Italy. Despite the sampling limitations, the results showed a temporal change in environmental awareness and pro-environmental behaviours. This finding could be linked to the greater availability and accessibility of information related to the marine environment, joined with the growing urgency to face environmental challenges that involve different sectors of the population. Despite the improvements reached in public education, efforts should still be undertaken to engage citizens, especially in inland areas, to disseminate the impact of daily actions on the marine environment and to increase the acceptability of and efforts in MPAs’ implementation and effective management.

Loss of Sunda clouded leopards and forest integrity drive potential impacts of mesopredator release on vulnerable avifauna

Amongst the unintended consequences of anthropogenic landscape conversion is declining apex predator abundance linked to loss of forest integrity, which can potentially re-order trophic networks. One such re-ordering, known as mesopredator release, occurs when medium-sized predators, also called mesopredators, rapidly increase in abundance following the decline in apex predator abundance, consequently reducing the abundance of mesopredator prey, notably including terrestrial avifauna. We examine the cascading impacts of declining Sunda clouded leopard abundance, itself consequent upon a reduction in forest integrity, on the mesopredator community of Sabah, Malaysia, to determine whether the phenomenon of mesopredator release is manifest and specifically whether it impacts the terrestrial avifauna community of pheasants and pittas. To explore this trophic interaction, we used a piecewise structural equation model to compare changes in the relative abundance of organisms. Our results suggest that loss of forest integrity may have broad impacts on the community and trigger mesopredator release, the two acting additively in their impact on already vulnerable species of terrestrial avifauna: a result not previously documented in tropical systems and rarely detected even on a global scale. The limiting effect that the Sunda clouded leopard has on the Sunda leopard cat could illuminate the mechanism whereby mesopredator release impacts this system. Both Bulwer's pheasant and pittas appear to be significantly impacted by the increase in Sunda leopard cats, while the great argus pheasant shows similar compelling, although not statistically significant, declines as Sunda leopard cats increase. The inverse relationship between Sunda clouded leopards and Sunda leopard cats suggests that if a mesopredator release exists it could have downstream consequences for some terrestrial avifauna. These results suggest the under-studied interface between mammalian carnivores and avifauna, or more broadly species interactions in general, could offer important conservation tool for holistic ecosystem conservation efforts.

An epigenetic toolbox for conservation biologists.

Ongoing climatic shifts and increasing anthropogenic pressures demand an efficient delineation of conservation units and accurate predictions of populations' resilience and adaptive potential. Molecular tools involving DNA sequencing are nowadays routinely used for these purposes. Yet, most of the existing tools focusing on sequence-level information have shortcomings in detecting signals of short-term ecological relevance. Epigenetic modifications carry valuable information to better link individuals, populations, and species to their environment. Here, we discuss a series of epigenetic monitoring tools that can be directly applied to various conservation contexts, complementing already existing molecular monitoring frameworks. Focusing on DNA sequence-based methods (e.g. DNA methylation, for which the applications are readily available), we demonstrate how (a) the identification of epi-biomarkers associated with age or infection can facilitate the determination of an individual's health status in wild populations; (b) whole epigenome analyses can identify signatures of selection linked to environmental conditions and facilitate estimating the adaptive potential of populations; and (c) epi-eDNA (epigenetic environmental DNA), an epigenetic-based conservation tool, presents a non-invasive sampling method to monitor biological information beyond the mere presence of individuals. Overall, our framework refines conservation strategies, ensuring a comprehensive understanding of species' adaptive potential and persistence on ecologically relevant timescales.

The Acoustic Index User's Guide: A practical manual for defining, generating and understanding current and future acoustic indices

Abstract Ecoacoustics, the study of environmental sound, is a rapidly growing discipline offering ecological insights at scales ranging from individual organisms to whole ecosystems. Substantial methodological developments over the last 15 years have streamlined extraction of ecological information from audio recordings. One widely used set of methods are acoustic indices, which offer numerical summaries of the spectral, temporal and amplitude patterns in audio recordings. Currently, the specifics of each index's background, methodology and the soundscape patterns they are designed to summarise, are spread across multiple sources. Critically, details of index calculation are sometimes scarce, making it challenging for users to understand how index values are generated. Discrepancies in understanding can lead to misuse of acoustic indices or reporting of spurious results. This hinders ecological inference, replicability and discourages adoption of these tools for conservation and ecosystem monitoring, where they might otherwise provide useful insight. Here we present the Acoustic Index User's Guide—an interactive RShiny web app that defines and deconstructs eight of the most commonly used acoustic indices to facilitate consistent application across the discipline. We break the acoustic indices calculations down into easy‐to‐follow steps to better enable practical application and critical interpretation of acoustic indices. We demonstrate typical soundscape patterns using a suite of 91 example audio recordings: 66 real‐world soundscapes from terrestrial, aquatic and subterranean systems around the world, and 25 synthetic files demonstrating archetypal soundscape patterns. Our interpretation figures signpost specific soundscape patterns likely to be reflected in acoustic indices' values. This RShiny app is a living resource; additional acoustic indices will be added in the future through collaboration with authors of pre‐existing and new indices. The app also serves as a best‐practice template for the information required when publishing new acoustic indices, so that authors can facilitate the widest possible understanding and uptake of their indices. In turn, improved understanding of acoustic indices will aid effective hypothesis generation, application and interpretation in ecological research, ecosystem monitoring and conservation management.

Detection and monitoring of the fauna of medium and large mammals using camera traps in a forest remnant area in the municipality of Água Fria de Goiás, Brazil – A tool for nature conservation

Despite the growing amount of information available, biodiversity is still under threat. Wildlife plays a crucial role in maintaining the ecological balance, and it is of logical and strategic importance to promote the further development of fauna recording and monitoring processes, emphasizing the need to strengthen the relationship between humans and nature. The aim was to record the species of medium and large mammals using camera traps. The study was conducted in the EcoCerrado Reserve in the municipality of Água Fria de Goiás, Brazil, between December 2020 and December 2022, using two camera traps in three different phytophysiognomies. 17 mammal species were recorded in 149 camera trap records, including species in the threatened category, such as Myrmecophaga tridactyla, Tapirus terrestrials, Chrysocyon brachyurus, Lycalopex vetulus, Panthera onca and Alouatta caraya. The mammal fauna observed in the study shows that this ecosystem can support populations of native species even in a modified landscape. This finding strengthens the basis for future initiatives aimed at environmental education, ecotourism, and more comprehensive legal protection for this region and other remnants of native vegetation in the Cerrado.

A model for the noninvasive, habitat-inclusive estimation of upper limit abundance for synanthropes, exemplified by M. fascicularis.

Accurately estimating population sizes for free-ranging animals through noninvasive methods, such as camera trap images, remains particularly limited by small datasets. To overcome this, we developed a flexible model for estimating upper limit populations and exemplified it by studying a group-living synanthrope, the long-tailed macaque (Macaca fascicularis). Habitat preference maps, based on environmental and GPS data, were generated with a maximum entropy model and combined with data obtained from camera traps, line transect distance sampling, and direct sightings to produce an expected number of individuals. The mapping between habitat preference and number of individuals was optimized through a tunable parameter ρ (inquisitiveness) that accounts for repeated observations of individuals. Benchmarking against published data highlights the high accuracy of the model. Overall, this approach combines citizen science with scientific observations and reveals the long-tailed macaque populations to be (up to 80%) smaller than expected. The model's flexibility makes it suitable for many species, providing a scalable, noninvasive tool for wildlife conservation.

Marine protected areas and fishing community well-being: An example from statewide socioeconomic monitoring of the California MPA network

As marine protected areas (MPAs) continue to proliferate as an ocean conservation tool, it is important to understand their socioeconomic outcomes, both in the short-term following implementation and in the long-term as they become a more permanent part of the ocean landscape. This paper presents results from an effort to conduct long-term socioeconomic monitoring of commercial fishing communities in relation to California's statewide MPA network, which was fully implemented in 2012 and consists of 124 MPAs covering 16% of state waters. To assess commercial fishing community perceptions of well-being and outcomes from the MPA network, we held virtual focus groups in 18 major port-based commercial fishing communities across California with a total of 85 participants from 2020 to 2021. Focus groups had both quantitative and qualitative components, with participants rating their fishing community and engaging in discussions on topics related to their environmental, economic, and social well-being and MPA outcomes. Results revealed that perceptions of fishing community well-being varied throughout the state, but were overall low. Key areas for improvement included recruitment and retention of participants in the fishing industry, availability and maintenance of key fishing infrastructure, and access to harvestable resources. Fishing community perceptions of the management and outcomes from the MPA network were overwhelmingly low across the state with little variation. Common reasons for participant dissatisfaction included perceptions of negative ecological and livelihood outcomes from the MPAs and a perceived lack of clear MPA management goals in addition to poor communication of those goals. Overall findings highlight the value of collecting community well-being data in coordination with long-term socioeconomic MPA monitoring to understand the greater context in which MPAs are received. A well-being approach to socioeconomic MPA monitoring also broadens policy pathways that could help improve outcomes from and beyond MPAs to support overall fishing community health.

Cryopreservation of date palm tissue cultures by vitrification and assessment of genetic stability using ISSR analysis

In this work, a successful cryopreservation method of date palm tissue cultures by vitrification was established. Callus and somatic embryos of three date palm cultivars were exposed to dimethylsulfoxide (DMSO) and Plant Vitrification Solution 2 (PVS2) for different durations before plunging into liquid nitrogen for cryoprotection. After freezing phase, the cryopreserved cultures were thawed and cultured on recovery media. Among different times of exposure to DMSO (10%), 60 min gave best results of survival and regrowth of the date palm tissue cultures. Regarding recovery, ‘Zaghlool’ cultivar gave the highest values of callus fresh mass (0.5 g) and growth value (1.00). Also, the highest differentiation percentages (75%) and number of proliferated shootlets (8.50) were recorded with ‘Zaghlool’ cultivar. Concerning PVS2, survival and regrowth gradually increased as exposure time increasing till 30 min and then decreased. Unlike of callus cultures, the survival and regrowth rates of somatic embryos were relatively higher at all PVS2 exposure time. With regard to recovery, the genotype effect took same trend in cryopreservation of callus cultures, since ‘Zaghlool’ was superior in growth parameters in comparison to the other two cultivars. On the other hand, genetic stability of the cryopreserved date palm tissue cultures was assessed using ISSR markers. Among four screened primers, three gave monomorphic bands; all of these banding profiles were similar to those of non-cryopreserved cultures. Generally, the results of ISSR showed high genetic similarity suggesting that cryopreservation using vitrification does not affect genetic stability of date palm tissue cultures. The results of this study demonstrated PVS2 was more effective in cryoprotection of date palm tissue cultures compared with DMSO and cryopreservation by vitrification is a suitable tool for conservation of genetic stable date palm germplasm.

Co-Producing an Ethnobotanical Garden to Support the Conservation of Indigenous Crop Diversity

Botanical gardens play a crucial role in documenting and sustaining traditional ecological knowledge (TEK) that were integral to the lives of Indigenous peoples. TEK has gained significant attention in discussions on sustainable development. Faced with threats to the maintenance and transfer of this knowledge, alternative approaches like community-based ethnobotanical gardens are emerging as effective tools for conservation. This paper details a research partnership that focused on storing and sharing the Bunun ethnic community’s TEK to conserve and promote plant and crop diversity. This collaboration further led to the co-development of an Indigenous ecological calendar detailing knowledge about crops, specifically beans. The ecological calendar emerged as an effective tool for supporting knowledge sharing, facilitating the communication of crop knowledge along with both common and scientific names. The Indigenous ecological calendar has also become a valuable tourism resource for guided tours, helping to build recognition of Indigenous knowledge, and making it accessible to future generations.

Collaborative Management on the Eastern Slopes: The Waldron Ranch Grazing Cooperative and Conservation Easement Motivations

Biodiversity conservation is at an inflection point. With the crises of biodiversity loss and climate change, novel strategies are needed to conserve ecosystems under a variety of property regimes. In southwestern Alberta, the Waldron Ranch Grazing Cooperative and the Nature Conservancy of Canada (NCC) have collaborated to use conservation easements (CEs) to place over 30,000 acres (12,140 hectares) of endangered grassland under perpetual protection. Waldron Ranch provides a unique case study, not only due to the size of the total CE, but also the cooperative structure that requires 72 Albertan ranchers to agree on restricting their productivity for conservation protections. We interviewed four individuals from the Cooperative and NCC to understand the motivations, values, and impacts that influenced the CE placement. Key themes include historic sustainable management and minimal impacts to livelihoods, with economic benefits being crucial for CE enrollment, which contrasts with findings from existing literature. Considering these, we expand on the critical role of landowner values and fears when using CEs as a tool for private land conservation. We argue that environmental nongovernmental organizations (eNGOs) need to deeply understand the social complexities that exist on private landscapes if voluntary collaborations are pursued for conservation benefits.

Implications of Microplastic Pollution for the Conservation of Marine Protected Areas Authors

Marine Protected Areas (MPAs) function as a tool for the protection and conservation of marine ecosystems. These designated areas should be free of any environmentally harmful pollutants. Microplastics (MPs) are plastic fragments measuring less than 5 mm (about 0.2 in). These fragments are an emerging threat to our oceans, and we are investigating the effectiveness of MPAs against these pollutants. We analyzed data gathered from research conducted on microplastic concentrations in MPAs and non-MPAs around the world. 53 MPAs and 53 non-MPAs around the world were used and the microplastic concentrations were deemed low, medium, or high by using the classifications established by the researchers. The Marine Protection Atlas (MPATLAS), created by the Marine Conservation Institute, was used to identify the levels of protection of each MPA and confirm the size of the protected area. The population density of cities nearest to an MPA or non-MPA was obtained by dividing the city’s population by the total area. Microplastics were found in all 106 sites researched, including waters near Antarctica and the northern waters of Greenland. When compared to non-MPAs, MPAs showed a higher number of areas with a “high” or “medium” concentration of microplastics. This indicates that Marine Protected Areas are an inefficient conservation tool against microplastic pollution. Population density did not appear to have a relationship with microplastic concentrations in MPAs and non-MPAs. The prominence of microplastics in protected marine ecosystems highlights the urgency to uncover their effects and a way to combat these anthropogenic pollutants.

Apex predators can structure ecosystems through trophic cascades: Linking the frugivorous behaviour and seed dispersal patterns of mesocarnivores

Abstract Current global change scenarios demand knowledge on how anthropogenic impacts affect ecosystem functioning through changes in food web structure. Frugivorous mesocarnivores are a key link in trophic cascades because, while their abundance and behaviour are usually controlled by apex predators, they can provide high‐quality seed‐dispersal services to plant communities. Thus, the recent rewilding of large carnivores worldwide can trigger cascading effects for plants. We investigated the top‐down effects of an apex predator (Iberian lynx Lynx pardinus) on seed‐dispersal services mediated by two mesocarnivore species (red fox Vulpes vulpes and stone marten Martes foina) at the plant community level by comparing areas with and without lynx in a Mediterranean mountain range in Southern Spain. We collected scats of mesocarnivores (n = 1575) to assess frugivory and seed dispersal of 15 plant species over two consecutive fruiting seasons and two habitat types (open and forest). Specifically, we assessed the effect of lynx presence on (i) seed occurrence and fleshy‐fruit biomass per scat, (ii) number of scats containing seeds and (iii) diversity of dispersed seeds. The quantity and diversity of dispersed seeds drastically decreased under predation risk for both mesocarnivore's species. Seed dispersal by stone martens was negatively affected by the presence of lynx, with a marked reduction in the number of scats with seeds (93%) and the diversity of dispersed seeds (46%). Foxes dispersed 68% fewer seeds in open habitats when coexisting with lynx, probably leading to differential contributions to seed‐dispersal effectiveness among habitats. Our study reveals a novel trophic cascade from apex predators to plant communities. The behavioural responses of frugivorous mesocarnivores to predation risk and the reduction in the intensity of their faecal deposition pattern are probably related to their lower abundance when co‐occurring with apex predators. While rewilding apex predators is a successful conservation tool, attention should be paid to cascading effects across food webs, particularly where frugivore megafauna is missing and mesocarnivores provide unique services to plants. Read the free Plain Language Summary for this article on the Journal blog.

Exploring crop maturity times as a conservation tool for improving resilience to human-elephant conflict: elephant crop-raiding

ABSTRACT The purpose of this study was to alleviate negative impacts of elephant crop-raiding faced by smallholder farmers around the Okavango delta. Although many studies have identified the relationship between crop-raiding and climatic factors, little attention was paid to aligning cropping duration to high rainfall. Therefore, this study aimed to investigate how crop maturity duration influences farmers’ vulnerability to elephant crop raiding and food loss. An experimental plot consisting of early and late maturing varieties of maize, sorghum and cowpea was set up and monitored in farms adjacent to corridors. Early maturing varieties yielded more (mean = 0.35 tha−1) than later maturing varieties (mean = 0.25 tha−1). The prolonged stay of crops in fields exposed them more to elephants (p < 0.001). Unlike late varieties, early varieties served as an evasion strategy by allowing earlier harvesting when there was adequate rainfall and elephants rarely frequented farms. Late maturing varieties were harvested at 16–19 weeks when elephants frequented the fields. This harvest timing significantly influences the vulnerability of crops to debilitating elephant raiding. This outcome suggests that policy reviews should incorporate more climate action into smallholder farming systems to strengthen SDG 1: no poverty and SDG 2: zero hunger, ease hostility toward elephants and improve elephant conservation.

Navigating divergent perspectives on critical habitat designation: Insights from the little bustard (Tetrax tetrax) conservation in Spain

The designation of critical habitats is a conservation tool extensively used worldwide. Although the effectiveness of this tool has been proved globally, its implementation in Spain is scarce, probably due to the ambiguous character of its definition in policy and other related problems. This study provides insight into some of these aspects and how different groups of experts interpret the concept of critical habitat. We used the little bustard (Tetrax tetrax) as a case study of an endangered species for which critical habitats must be designated. We interviewed 47 experts of the species, from researchers to environmental agency technicians, about the objectives that critical habitats should address, and which facilitating and constraining aspects should be considered in such designation. By grouping the answers in different categories and running ordination analyses (Non-Metric Multidimensional Scaling) we found that researchers mainly focused on ecological objectives for critical habitats, compared to the rest of the respondents who also considered socioeconomic aspects as basic objectives for critical habitats. Only 36% of the experts mentioned ecological factors as constraints to such designation, while socioeconomic constraints were mentioned by all but two of the experts (96%). These results suggest differences in the conceptualization of critical habitat between scientists and managers, from pure ecological requirements of the species to the actual implications and socioeconomic constraints of its implementation, respectively. Integrating multiple stakeholders and views will reflect these differences into the operational understanding of critical habitats and will ensure that future critical habitat designations are effective management tools.