Despite the increasing popularity of glamping structures, empirical studies often overlook the carbon impact of wood in these constructions, creating a significant research gap. Understanding the net carbon effect of wood in glamping structures is crucial for informing sustainable building practices. This paper aims to quantitatively compare the net carbon impact of wood in glamping structures, filling a notable gap in the current research literature. The investigation undertakes a thorough evaluation employing a life cycle methodology, appraising the emissions linked with the complete glamping life span. Seven Romanian companies are examined vertically within the glamping production chain and horizontally across the supply value chain. The investigation unveils a notable discovery: the integration of wood within glamping yields considerable carbon sequestration, wherein the wood employed sequesters 36.83 metric tons of CO2 per glamping unit. This surpasses the carbon emissions entailed throughout the entirety of the glamping life cycle, ranging from 9.97 to 11.72 metric tons of carbon. Remarkably, a single wood-incorporated glamping structure has the capacity to sequester approximately 25 metric tons of carbon within a span of 50 years. In summary, the investigation underscores the capacity of responsibly sourced timber to function as a carbon reservoir, proficiently counterbalancing emissions across the entirety of the construction life cycle. The findings underscore the importance of sustainably sourced wood in achieving carbon neutrality and provide valuable insights for promoting sustainable building practices. This methodology has broad applicability beyond glamping structures, holding potential for replication and scalability across various sectors and regions, thereby contributing to global efforts towards mitigating climate change and fostering positive environmental change.