Rat Tongue Research Articles

Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair?

The mechanisms of action of photobiomodulation (PBM) in oral mucositis (OM) are not completely elucidated. To enlighten the role of PBM in the evolution of epithelial maturity in OM ulcers, the present study evaluated the effect of PBM with red (λ) wavelength of 660 nanometers (nm) and infrared of 780 nm in radio-induced OM wounds on the tongue of rats, eight and twenty days after irradiation with single dose of 20 Gy. The percentage area corresponding to positive staining for cytokeratin 10 (CK10) and 14 (CK14) proteins was evaluated in the epithelial area of the lesions, using an immunohistochemical technique (IHC), 8 and 20 days after the induction of lesions, and compared with an untreated control group. CK10 was significantly more expressed in the group treated with 660 nm PBM. CK14 did not show quantitative differences between the groups evaluated. However, whereas in the groups treated with PBM, CK14 was already restricted to the basal layer of the epithelium, as expected in healthy epithelia, in control group it was also expressed in upper layers of the epithelium. In this work, PBM was able to improve epithelial maturity of the repaired OM wound, especially in the 660 nm group.

Morphological structure of rat tongue using light and scanning electron microscopy.

The rat is one of the most commonly used animals in biological research and experimental investigations in medicine. The ultrafine structural components of the epithelium differ depending on the anatomy of the animal and the papilla type. Animal adaptation to food types and environmental circumstances may also be linked to morphological diversity. In the current study, seven male Wistar rat tongues were investigated. For scanning electron microscope (SEM), two rat tongues were immersed in a 10% formalin solution and the other two rat tongues were immersed in a 2.5% glutaraldehyde solution. The tongues of three rats were fixed for regular histological evaluation using triple staining. The three primary components of the Wistar rat tongue are the apex, body and root. The apex had a rounded and bifurcated shape. Filiform papillae and gustatory papillae were easily identified on the dorsal side of the tongue. There were three forms of gustatory papillae; fungiform papillae, vallate papillae and foliate papillae. The purpose of this study was to expose the tongue morphology of the Wistar rat species, which is widely used in investigations. Also, we wanted to show that formalin fixation can be utilized for morphological research in SEM. Finally, the Wistar rat tongue was thoroughly investigated and compared to those of other species.

Macroglossia and less advanced dystrophic change in the tongue muscle of the Duchenne muscular dystrophy rat

BackgroundDuchenne muscular dystrophy (DMD) is an X-linked muscle disease caused by a complete lack of dystrophin, which stabilizes the plasma membrane of myofibers. The orofacial function is affected in an advanced stage of DMD and this often leads to an eating disorder such as dysphagia. Dysphagia is caused by multiple etiologies including decreased mastication and swallowing. Therefore, preventing the functional declines of mastication and swallowing in DMD is important to improve the patient’s quality of life. In the present study, using a rat model of DMD we generated previously, we performed analyses on the masseter and tongue muscles, both are required for proper eating function.MethodsAge-related changes of the masseter and tongue muscle of DMD rats were analyzed morphometrically, histologically, and immunohistochemically. Also, transcription of cellular senescent markers, and utrophin (Utrn), a functional analog of dystrophin, was examined.ResultsThe masseter muscle of DMD rats showed progressive dystrophic changes as observed in their hindlimb muscle, accompanied by increased transcription of p16 and p19. On the other hand, the tongue of DMD rats showed macroglossia due to hypertrophy of myofibers with less dystrophic changes. Proliferative activity was preserved in the satellite cells from the tongue muscle but was perturbed severely in those from the masseter muscle. While Utrn transcription was increased in the masseter muscle of DMD rats compared to WT rats, probably due to a compensatory mechanism, its level in the tongue muscle was comparable between WT and DMD rats and was similar to that in the masseter muscle of DMD rats.ConclusionsMuscular dystrophy is less advanced in the tongue muscle compared to the masseter muscle in the DMD rat.

Evaluation of lingual mucosa toxicity and recovery follow-up in rats, following sub-chronic exposure to titanium dioxide nanoparticles.

Nanosized titanium dioxide (TiO2) particles are among the most widely used nanoparticles (NPs) worldwide due to their unique properties. The lingual mucosa is still neglected in terms of risk assessment with respect to the NP uptake. The aim of this study was to evaluate the effect of intragastric administration of TiO2 NPs on the mucous membranes of the tongues of albino rats, as well as the potential benefits of a 4-week recovery period. Twenty-one male albino rats were randomly divided into 3 groups (n = 7 per group). Group I served as a control group and received saline intragastrically daily for 30 days. The experimental groups included group II, which received TiO2 NPs in the amount of 50 mg/kg of body weight (b.w.) intragastrically daily for 30 days, and group III, which also received TiO2 NPs in the amount of 50 mg/kg b.w. intragastrically daily for 30 days, and then was allowed to recover for 4 weeks. Tongue specimens were collected for histological, immunohistochemical and morphometric examinations. The TiO2 NP group II showed significant atrophic and degenerative changes in the tongue mucosa, which included reduced epithelial thickness in the ventral surface, with disfigurement in the filiform and fungiform papillae. Weak B cell lymphoma-2 (Bcl-2) immunoreactivity was also noted. The 4-week recovery group displayed improvement in the histological picture, with moderate to strong Bcl-2 immunoreactivity in the epithelial cell layers and the underlying connective tissue. The use of TiO2 NPs caused severe histological and apoptotic changes in the filiform and fungiform papillae, and the ventral surface of the tongue of the rats, while allowing recovery minimized the toxic effect of the NPs.

Morphological, ultrastructural, and biochemical changes induced by sodium fluoride in the tongue of adult male albino rat and the ameliorative effect of resveratrol.

Little knowledge is available about the effects of fluoride exposure on the tongue. This study evaluated the effects of sodium fluoride (NaF) on the tongue ultrastructure and detected the ameliorative effects of resveratrol. Forty adult albino rats were separated into 4 groups: the control group was given a balanced diet and purified water. The NaF treated group: received 10 mg/kg/d dissolved in 2.5 ml distilled water once daily for 30 days orally. The NaF+resveratrol group: received NaF 10 mg/kg/d orally together with resveratrol in a dose of 30 mg/kg daily for 30 days. The resveratrol group was subjected to resveratrol in a dose of 30 mg/kg/d by oral gavage for 30 days. Sections were stained with hematoxylin & eosin, and Masson's trichrome. Tumor necrosis factor α immunohistochemical study and electron microscopic examinations were done. The oxidative stress markers malondialdehyde, antioxidant reduced glutathione, and the total antioxidant capacity were measured. The NaF group revealed ulceration, necrotic muscle fibers, distorted papillae and a significant increase in malondialdehyde level, and a significant decrease in glutathione and the total antioxidant levels. In the NaF+resveratrol group, pathological changes were less, and the oxidant levels were decreased by the administration of resveratrol with NaF. In conclusion, NaF adversely affects the ultrastructure of the adult rat tongue and resveratrol can ameliorate this effect.

Morphometric Features and Microanatomy of the Lingual Filiform Papillae in the Wistar Rat.

Simple SummaryThe tongue plays an important role in all animals but especially in mammals. It participates in food and water intake, as well as in some behavioral activities such as grooming. Its structure differs depending on the species. We aimed to provide a detailed macroscopic and microscopic description of the filiform papillae on the surface of rat tongue. We examined fragments on three regions of the tongue (tip, body, root) and observed an intensely keratinized epithelium on the whole surface of the tongue, with higher keratinization on the filiform papillae. We also identified differences in the density of the papillae dependent on the region examined, with the highest density being present on the back of the tongue. Additionally, we noted differences in the height of the filiform papillae, with the shortest being on the tip of the tongue and the tallest on the middle of the tongue. The unusual posteroanterior inclination of the filiform papillae from the tongue protuberance suggests they may play some other obscure roles compared to anteroposteriorly oriented filiform papillae. The study of macro and microanatomy in mammals is important since it helps in the assessment and understanding of anatomical and behavioral features of species.The mammalian tongue plays a fundamental role in various physiological and behavioral activities. Significant morphological variations have been recorded in the tongue of several species. This study aims to obtain detailed histological and morphometric information about the filiform papillae on the surface of rat tongue. The tongues of five 10-month-old Wistar rats were utilized, which were later examined with a stereo-microscope. Fragments from the three regions of the tongue were collected for histological investigations. The tongue of the Wistar rat has an intensely keratinized stratified squamous epithelium, with the highest degree of keratinized epithelium covering the filiform papillae. The filiform papillae differ in density, with the highest density recorded on the posterior part of the lingual body and the lowest density on the protuberance. The shortest filiform papillae were observed on the apex of the tongue and the tallest on the anterior part of the lingual body. Interestingly, the orientation of the filiform papillae on the lingual protuberance was inclined posteroanteriorly, in the opposite way as compared to the papillae from all the other regions of the tongue. Histologically, a difference was recorded in the structure of the covering epithelium of the anterior vs. the posterior face of the filiform papillae.

Effect of chronic ethanol ingestion on dendritic cell population during oral mucosal repair: An experimental study.

The aim of this study was to analyze the effect of chronic ethanol ingestion on dendritic cell repopulation during the repair process of rat oral mucosa and in the rat spleen by analyzing the immunohistochemical expression of dendritic cell markers. Wistar rats ingested 20% ethanol solution for 28 days; a surgical wound was performed on the rat tongue after this period. The repair process and the number of CD1a+, CD11c+, and CD207+ cells in the regions adjacent to the wound were determined at day 1, 3, and 7 following the wound as well as in the rat spleen. The wound-only group (no ethanol exposure) had complete reepithelization after 7 days, but this did not occur in the ethanol + wound group at this time point. The inflammatory infiltrate was significantly reduced in animals exposed to ethanol, which also showed significantly lower counts of CD1a+, CD11c+, and CD207+ cells than the wound-only group at all experimental time points. In addition, ethanol exposure also resulted in lower densities of CD11c+ and CD207+ cells in the rat spleen. In conclusion, chronic ethanol intake had a negative impact on dendritic cell numbers, a fact that may contribute to delay in oral mucosa repair.

The Influence of Roux-en-Y Gastric Bypass and Diet on NaCl and Sucrose Taste Detection Thresholds and Number of Circumvallate and Fungiform Taste Buds in Female Rats.

Roux-en-Y gastric bypass (RYGB) in rats attenuates preference for, and intake of, sugar solutions. Additionally, maintenance on a high-fat diet (HFD) reportedly alters behavioral responsiveness to sucrose in rodents in short-term drinking tests. Due to the fact that the behavioral tests to date rely on the hedonic value of the stimulus to drive responsiveness, we sought to determine whether taste detection thresholds to sucrose and NaCl are affected by these manipulations as measured in an operant two-response signal detection paradigm. Female rats were maintained on HFD or chow for 10 weeks, at which point animals received either RYGB or SHAM surgery followed by a gel-based diet and then powdered chow. Upon recovery, half of the rats that were previously on HFD were switched permanently to chow, and the other rats were maintained on their presurgical diets (n = 5–9/diet condition × surgery group for behavioral testing). The rats were then trained and tested in a gustometer. There was a significant interaction between diet condition and surgery on NaCl threshold that was attributable to a lower value in RYGB vs. SHAM rats in the HFD condition, but this failed to survive a Bonferroni correction. Importantly, there were no effects of diet condition or surgery on sucrose thresholds. Additionally, although recent evidence suggests that maintenance on HFD alters taste bud number in the circumvallate papillae (CV) of mice, in a subset of rats, we did not find that diet significantly influenced taste pores in the anterior tongue or CV of female rats. These results suggest that any changes in sucrose responsiveness in intake/preference or hedonically oriented tests in rats as a function of HFD maintenance or RYGB are not attributable to alterations in taste sensitivity.

Early Detection of Leukoplakic Oral Squamous Cell Carcinoma Using 4NQO-induced Rat Tongue Cancer Model: Study Utilizing Fluorescence Intensity and Histopathological Evaluation.

Early identification of leukoplakic oral squamous cell carcinoma (OSCC) is difficult. The purpose of this study was to determine whether it was possible to detect change from normal epithelium to leukoplakic OSCC using a fluorescence visualization (FV) device in a 4-nitroquinoline 1-oxide (4NQO) -induced rat tongue cancer model. If successful, this would facilitate early detection of OSCC. The rats (3 groups of 5) were administered 50 ppm 4NQO in their drinking water over a period of 10, 15, or 20 weeks. Five non-treated rats were used as a control group. Images of their tongues obtained by FV were analyzed for change in fluorescence intensity (FI) using image analysis software. Immunoreaction for anti-CK13, anti-CK17, and anti-E-cadherin antibodies was also histopathologically evaluated. Receiver operating characteristic (ROC) analysis was used to calculate the cut-off values, sensitivity, specificity, and area under the curve. The most marked change in FI was found between the control and 10-week groups, with an increase observed in its average value and range in the latter. These findings differed from those characteristic of leukoplakia. No significant difference was observed in the positive cell rate for immunoreaction for anti-CK13 or anti-CK17 antibodies between the control and 10-week groups. A significant decrease was observed in the positive pixel ratio of immunoreaction for anti-E-cadherin antibody in the 10-week group in comparison with in the control group (p <0.05). These results showed that disruption of intercellular adhesion could be observed at 10 weeks. In the ROC analysis, the FI cut-off value in the 10-week and control groups was 51.9, sensitivity 95.5%, and specificity 96.9%. This indicated that normal epithelium could be accurately distinguished from low-grade dysplasia with high probability. These results demonstrate that analysis of change in FI as measured by FV could facilitate early detection of leukoplakic OSCC.

Determination of Oxaliplatin by a UHPLC-MS/MS Method: Application to Pharmacokinetics and Tongue Tissue Distribution Studies in Rats

Oxaliplatin (OXP), a third-generation platinum-based chemotherapy drug, was often indirectly analyzed via total platinum by an ICP-MS because it was difficult to directly quantify using an LC-MS/MS method, due to its instability, bad column separability and severe MS signal inhibition. Here, we developed and validated a specific, sensitive and reproducible LC-MS/MS method for the quantification of OXP itself in rat plasma and tongue tissue on a SCIEX 4000 QTRAP® MS/MS system equipped with a Phenomenex Lux 5u Cellulose-1 column (250 × 4.6 mm, 5 μm). This method was validated at the lower limit of detection (LOD) and the lower limit of quantitation (LLOQ) of 5 ng/mL and 10 ng/mL, with linearity of 10–5000 ng/mL (r2 > 0.99) and 10–2500 ng/mL (r2 > 0.99), in rat plasma and tongue homogenates, respectively. The intra- and inter-day precision (CV%) and accuracy (RE%) were within 15% for LLOQ, low-, medium- and high-quality control samples. The mean extraction recoveries were around 50% and 80% for plasma and tongue homogenates, respectively. This assay was successfully applied to pharmacokinetics study following intravenous administration of OXP, as well as tongue tissue distribution after 1 h and 4 h of a novel oral mucosal patch application.

MORPHOMETRIC DESCRIPTION OF MUSCLES OF THE TONGUE UNDER THE EXPERIMENTAL COMBINED EFFECT OF FOOD ADDITIVES

The peculiarities of the structural organization of the tongue quite often become the object of research by domestic and foreign researchers. At the same time, most of the contemporary scientific works are devoted to the study of the structural features of the lingual mucosa. Changes in the muscles of the tongue under the influence of certain unfavorable factors, in most cases, are insufficiently described. Aim: experimental study of the morphometric parameters of muscles of the tongue of the albino rats in introduction of complex food additives (monosodium glutamate, sodium nitrite, Ponceau 4R) into the ration over a period of 16 weeks. 60 outbred albino rats were involved into the study. The ration of experimental animals was supplemented with complex food additives, namely, monosodium glutamate, Ponceau 4R, sodium nitrite for 1 and 4 weeks. Animals of the experimental groups, in addition to the standard diet, received a combination of food additives monosodium glutamate, ponceau 4R, sodium nitrate for 1, 4, 8, 12 and 16 weeks. The animals were sacrificed under thiopentone anesthesia overdose. Conventional histological microspecimens were made from tongue preparations, stained with hematoxylin and eosin. On the microspecimens, the ratio between the muscle and connective tissues, the diameter of the muscle fibers of the tongue on the tip, body and base were measured. The findings of the studies have shown that introduction of complex food additives (monosodium glutamate, sodium nitrite, Ponceau 4R) into the ration of laboratory animals for one week leads to an increase in the relative amount of muscle tissue in the tongue and to a heterogeneous change in the diameter of muscle fibers on the tip, body and base. The described parameters resulted from the changes in the nature of the movement of the tongue in the process of food mixing, when adapting to changes in the ration. Starting from week 4 of consumption of complex food additives supplemented into the ration of the laboratory animals, progressive atrophic changes were observed in the muscle fibers of all parts of the tongue, manifested by a gradual decrease in the diameter. At the same time, gradual decrease in the relative amount of muscle fibers and an increase in the relative amount of connective tissue were noted in the tongue of experimental animals.

Flexible Tongue Electrode Array System for In Vivo Mapping of Electrical Signals of Taste Sensation.

Tongue is a unique organ that senses tastes, and the scientific puzzle about whether electricity can evoke taste sensations and how the sensations have been distributed on the tongue has not been solved. Investigations on tongue stimulation by electricity might benefit the developments of techniques for clinical neuromodulation, tissue activation, and a brain-tongue-machine interface. To solve the scientific puzzle of whether electrical stimulation induces taste-related sensations, a portable flexible tongue electrode array system (FTEAS) was developed, which can synchronously provide electrical stimulation and signal mapping at each zone of the tongue. Utilizing the FTEAS to perform tests on the rat tongue in vivo, specific electrical signals were observed to be evoked by chemical and electrical stimulations. The features and distributions of the electric signals evoked during the rat tongue tests were systematically studied and comprehensively analyzed. The results show that an appropriate electrical stimulation can induce multiple sensations simultaneously, while the distribution of each sensation was not significantly distinguished among different zones of the tongue, and at the same time, this taste-related electrical signal can be recorded by the FTEAS. This work establishes a promising platform to solve the scientific puzzle of how sensations are activated chemically and electrically on the tongue and may provide advanced noninvasive oral-electrotherapy and a brain-tongue-machine interface.

Validation of RNA Aptamer Probes to Image Candida albicans in Paraffin-Embedded Sections of Wistar Rat Tongue.

Objective This study aimed to validate the use of Ca-apt-1, an RNA aptamer, that we generated previously as a probe for immunostaining of Candida albicans in rat tongue paraffin-fixed tissue sections Material and Methods The performance of Ca-apt-1 as a detector molecule was compared with that of anti- C. albicans polyclonal antibody (PcAb), which was used as a positive control. Immunostaining images were visualized by light microscopy and were analyzed by using ImageJ software. Results Microscopic results demonstrated that Ca-apt-1 specifically recognized and immunostained C. albicans cells of rat tongue candidiasis, with a specificity comparable to that of PcAb. ImageJ analysis showed that the area (pixels) detected by Ca-apt-1 was wider than that detected by the antibody. This indicates that the binding affinity of Ca-apt-1 toward C. albicans was better than that of PcAb on paraffin-embedded tissues. Conclusion This study demonstrates that Ca-apt-1 can be used as a probe for immunostaining of fixed tissue sections for oral candidiasis diagnosis.

Impact of electronic cigarette vaping on the cytoarchitecture of the tongue in the adult male albino rat

Impact of electronic cigarette vaping on the cytoarchitecture of the tongue in the adult male albino rat

Histopathological changes in submandibular gland and dorsal tongue of experimental rats due to prolonged tramadol intake focusing on novel modulatory effect of 10-dehydrogingerdione

ObjectivesThis project aims to develop a framework to illustrate the degenerative effects induced by prolonged tramadol intake in salivary glands and tongue tissues. We strive in this work to investigate the probable role of 10-dehydrogingerdione (10-DHGD) in regeneration of these tissues. DesignForty male albino rats were designated for the study and categorized into four groups. Group (1) received no drugs and served as normal control group. Group (2) received tramadol intra peritoneal (20 mg /kg) body weight daily for 45 days. Group (3) received freshly prepared 10-DHGD orally in a dose level (10 mg /kg). Group (4) received combination of tramadol and 10 DHGD for 45 days. Histological examination is that routine testing that was done in all studied subjects to demonstrate any cytological changes with hematoxylin and eosin (H&E) in the submandibular glands and dorsal tongue tissues along with histochemical investigation using periodic acid-Schiff (PAS) and immunohistochemical presentation of Caspase-3. ResultsSubmandibular salivary glands and dorsal tongue tissues showed degenerative changes in tramadol treated group while control and 10-DHGD groups presented with no cytological or morphological changes. Histochemical investigation revealed marked reduction in PAS staining reaction in tramadol group as compared to other studied groups. Regarding to immunoreactivity of caspase-3 when all groups were compared, the differences in mean values of area percentage were statistically significant. ConclusionsTramadol provoked oxidative damage and apoptosis in oral tissues, which significantly decreased by 10-DHGD intake as it may exert an ameliorative effect that help alleviating these degenerative effects.

Glycyrrhiza glabra root extract alleviates cyclophosphamide induced mucositis of the tongue in adult male albino rats

ABSTRACTIntroduction: Cyclophosphamide (CPA) is an anti-cancer medication, utilized in chemotherapy. Recently, inflammation of oral mucosa (mucositis) is thought to be one of the most serious side effects of anticancer therapy. Aim of the work: To investigate the role of glycyrrhiza glabra (Licorice) root extract on rat tongue mucositis induced by CPA.Materials and methods: Forty adult male albino rats were classified into four groups, (each included 10 rats): Group I (Control group), Group II (Licorice group): each rat received licorice root extract by an oral daily dose of 200 mg/kg/day for 10 days, Group III (CPA group): each rat received CPA in a single intraperitoneal dose of 300 mg/kg on the second day of experiment and Group IV (Licorice CPA group): each rat received licorice root extract by an oral daily dose of 200 mg/kg/day for ten days, on the second day of experiment, the rats received CPA in a single intraperitoneal dose of 300 mg/kg. At the end of the experiment, the rats were anesthetized, and the tongue was excised for histological, immunohistochemical and ultrastructural analysis. Morphometric and statistical analysis were also done.Results: Licorice root extract reduced the severity of CPA induced oral mucositis by increasing the epithelial thickness, normalization of congested blood vessels in the lamina propria of tongue mucosa, significant amelioration of immunoreactivity to anti-Ki-67, anti-E-cadherin and anti-P53.Conclusion: CPA chemotherapy has a detrimental effect on the oral mucosa resulting in marked morphometric and microscopic changes. Glycyrrhiza glabra extract can protect the oral mucosa from CPA-induced toxicity and reduce the associated injury.

Nociceptive receptors are expressed differently in trigeminal nociception after lingual nerve injury and unilateral external carotid artery occlusion in rats

ObjectivesTo investigate the different changes in nociceptive activity between two animal models of trigeminal neuropathic pain: unilateral external carotid artery ischemic reperfusion and lingual nerve crush in rats. DesignIn this study, changes in nociceptive activity were investigated in unilateral external carotid artery ischemic reperfusion and lingual nerve crush models of trigeminal neuropathic pain in rats. Field excitatory postsynaptic potentials (fEPSPs) evoked by capsaicin application on the tongue of rats were recorded in the trigeminal nucleus caudalis. In addition, immunohistochemistry was performed in the trigeminal ganglia and trigeminal nucleus caudalis. ResultsThe fEPSP in unilateral external carotid artery ischemic reperfusion and lingual nerve crush rats was irregular relative to that in sham rats. In particular, the fEPSP spike in lingual nerve crush rats had a higher amplitude and shorter duration than that in sham rats. Unilateral external carotid artery ischemic reperfusion and lingual nerve crush also increased c-fos expression in the trigeminal nucleus caudalis. Upregulation of transient receptor potential vanilloid 1 in trigeminal ganglion was observed in unilateral external carotid artery ischemic reperfusion and lingual nerve crush rats, whereas upregulation of purinergic receptor subtype 3 in trigeminal ganglion was observed only in lingual nerve crush rats. ConclusionsAlthough unilateral external carotid artery ischemic reperfusion and lingual nerve crush similarly increased nociceptive activity at the trigeminal nucleus caudalis, the fEPSPs and expression of nociceptive peripheral afferent neurons were different. Therefore, direct and indirect nerve injuries apparently induced the same nociceptive activity by different signaling responses dependent on nociceptive receptors.

Therapeutic efficacy of chrysin on acrylamide induced striated muscles dystrophy of rat's tongue (histological and histochemical study)

The aim of the present study was directed to evaluate the therapeutic efficacy of chrysin (CH) on acrylamide (ACR) induced striated muscles dystrophy of rat’s tongue. subjects and methods: Forty adult male albino rats (200–250 g) were divided into four equal group(s) (G(s)), used for two successive periods (20 days: A , 40 days: B). G1 were fed with standard diet, without receiving any kind of treatment and considered as negative control group. G2 were received 15 mg/kg body weight of ACR orally once daily for 20 days (G2A) and for 40 days (G2B) and considered as positive control group. G3 were treated as G2, in addition; they were received 50 mg/kg intraperitoneal of CH two weeks before, as well as during application of ACR on alternative days and considered as a chemopreventive group. G4 were treated as G2, in addition; they were received 50 mg/kg intraperitoneal of CH, at first, third and seventh days after application of ACR and considered as a treatment group. Results: G1 has no observable abnormalities. G2A and G2B revealed an increase in collagen fibers with degenerative changes. G3A and G3B revealed decreased collagen fibers with muscle regeneration. G4A revealed localized red swelling of tongue mucosa, diffuse in G4B. Both had limited areas of collagen fibers. Conclusion: Striated muscle damage in rat's tongue by ACR becomes worse with prolonged duration is realized. CH has a significant protective efficacy of when compared with that of treatment on ACR induced striated muscle injury of rat’s tongue.

Diffusion Reflection Method for Early Detection of Oral Squamous Cell Carcinoma Specifically Targeted by Circulating Gold-Nanorods Bio-Conjugated to Anti-Epidermal Growth Factor Receptor.

BackgroundTranslation of nanomedical developments into clinical application is receiving an increasing interest. However, its use for oral squamous cell carcinoma (OSCC) diagnosis remains limited. We present an advanced nanophotonic method for oral cancer detection, based on diffusion reflection (DR) measurements of gold-nanorods bio-conjugated to anti-epidermal growth factor receptor (C-GNRs) specifically attached to OSCC cells.ObjectiveTo investigate in a rat model of oral carcinogenesis the targeting potential of C-GNRs to OSCC by using the DR optical method.Materials and MethodsOSCC was induced by the carcinogen 4-nitroquinoline-N-oxide (4NQO). C-GNRs were introduced locally and systemically and DR measurements were recorded from the surface of the rat tongue following illumination with red laser beam. Rats were divided into experimental and control groups. The results were compared with the histologic diagnosis.ResultsA total of 75 Wistar-derived rats were enrolled in the study. Local application did not reveal any statistical results. DR measurements following intravenous injection of C-GNRs revealed a significant increase in light absorption in rats with OSCC compare with rats without cancer (p<0.02, sensitivity 100%, specificity 89%). In addition, absorption of light increased significantly in cases of severe dysplasia and cancer (high risk) compared to rats without cancer and rats with mild dysplasia (low risk) (86% sensitivity and 89% specificity, AUC=0.79).ConclusionCombining nanotechnology and nanophotonics for in vivo diagnosis of OSCC serves as additional tier in the translation of advanced nanomedical developments into clinical applications. The presented method shows a promising potential of nanophotonics for oral cancer identification, and provides support for the use of C-GNRs as a selective drug delivery.

Serum metabolite profiling of a 4-Nitroquinoline-1-oxide-induced experimental oral carcinogenesis model using gas chromatography-mass spectrometry.

BackgroundOral cancer progresses from hyperplastic epithelial lesions through dysplasia to invasive carcinoma. The critical needs in oral cancer treatment are expanding our knowledge of malignant tumour progression and the development of useful approaches to prevent dysplastic lesions. This study was designed to gain insights into the underlying metabolic transformations that occur during the process of oral carcinogenesis.MethodsWe used gas chromatography-mass spectrometry (GC-MS) in conjunction with multivariate statistical techniques to observe alterations in serum metabolites in a 4-Nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis model. Thirty-eight male rats were randomly divided into two groups, including the 4NQO-induced model group of 30 rats and the healthy control group of five rats. Animals were sacrificed at weeks 9, 13, 20, 24, and 32, post-4NQO treatment. Tissue samples were collected for histopathological examinations and blood samples were collected for metabolomic analysis. Partial least squares discriminate analysis (PLS-DA) models generated from GC-MS metabolic profile data showed robust discrimination from rats with oral premalignant and malignant lesions induced by 4NQO, and normal controls.ResultsThe results found 16 metabolites associated with 4NQO-induced rat tongue carcinogenesis. Dysregulated arachidonic acid, fatty acid, and glycine metabolism, as well as disturbed tricarboxylic acid (TCA) cycle and mitochondrial respiratory chains were observed in the animal model. The PLS-DA models of metabolomic results demonstrated good separations between the 4NQO-induced model group and the normal control group.ConclusionWe found several metabolites modulated by 4NQO and provide a good reference for further study of early diagnosis in oral cancer.