Tomography Features Research Articles

Modern View on Bronchiectasis in Children

Based on 31 publications the review provides relevant information on epidemiology, etiology, pathogenesis and pathomorphology, clinical, computed tomographic features, diagnostics, and differential diagnosis of bronchiectasis in children. Recommendations for treatment and management of children and adolescents with non-cystic fibrosis bronchiectasis, and prevention of bronchiectasis exacerbations are given from the standpoint of evidence-based medicine taking into account the guidelines of the European Respiratory Society 2021.

Non cystic fibrosis-related bronchiectasis in children: etiological structure, clinical and laboratory and computed tomographic characteristics

Aim. To establish the etiological structure and to present clinical and laboratory and instrumental characteristics of bronchiectasis (BE) not associated with cystic fibrosis (CF) in children.
 Materials and methods. Sixty-seven hospitalised patients with BЕ not related to CF were followed up between 2017 and 2022. Examination methods: clinical-anamnestic method, general clinical laboratory investigations, investigation of allergological and immune status, phagocytosis system, determination of concentration of specific IgE and IgG to fungi of genus Aspergillus, sweat test, radiological examination and computed tomography (CT) of chest organs, bronchoscopy, Bacteriological examination of sputum and/or tracheobronchial aspirates, nasal and/or bronchial ciliary motility, esophagogastroduodenoscopy, 24-hour pH-metry, intra-esophageal combined impedance-pH-metry, genetic study, lung biopsy.
 Results. Etiologic factors of BЕ not associated with CF in children were severe pneumonia (22%), primary ciliary dyskinesia (22%), bronchial asthma (13%), Williams-Campbell syndrome (7%), bronchial foreign bodies (7%), gastroesophageal reflux disease (6%), Bronchopulmonary dysplasia (6%), postinfectious bronchiolitis obliterans (5%), allergic bronchopulmonary aspergillosis (3%), chronic granulomatous disease (3%), AIDS (1%), prolonged bacterial bronchitis (1%), brain-lung-thyroid syndrome (1%). The clinical picture is characterized by cough (91%), shortness of breath (67%), fever during exacerbation (48%), chest pain (24%), exercise intolerance (55%), drumstick symptom (9%), moist (76%) and dry wheezing (37%). CT-semiotics of BЕ not associated with CF is characterized by localization in one (58%) or several (42%) lobes; traction (42%), non-traction (49%) B and their combination (9%); increased broncho-arterial ratio 0.9; thickening of bronchial wall; "mosaic perfusion"/"air-trap" symptom (9%); more frequent involvement of lower lungs (64%). The main infectious agents in BЕ not associated with CF were Haemophilus influenzae, Pseudomonas aeruginosa, Staphylococcus aureus.
 Conclusion. On the basis of a multicentre study, the etiological structure, clinical and laboratory and CT-characteristics of non-CF ВE in children were established.

Radiographic, Computed Tomographic, and Cellular Phenotypic Features of Primary Nasal Transmissible Venereal Tumors in Four Dogs

The primary nasal canine transmissible venereal tumor (CTVT) is a rare disease that develops by the allografted transmission of neoplastic cells in the nasal cavity. The disease is uncommonly reported in free-roaming dogs, with the social behavior of excessive licking and vigorously sniffing the affected parts of the other dogs in an endemic population. Post-chemotherapeutic computed tomography (CT) scan features and correlation of vincristine sulfate with cellular phenotypes have been scarcely reported in previously available primary nasal CTVT studies. This study describes the radiographic, computed tomographic, and cellular phenotypic features in four dogs affected with stage-4 intranasal CTVTs. The post-chemotherapeutic features of the nasal cavity in fully recovered cases are also highlighted. All data were analyzed retrospectively. All four dogs had stage 4 modified Adam’s staging for nasal tumors due to the complete or partial lysis of the cribriform plate and lymphocytoid plasmacytoid (mixed) phenotype of the neoplastic cells based on the cellularity of cytological samples. All four dogs responded well to five cycles of vincristine sulfate and recovered completely. Two out of four dogs have follow-up scanning after chemotherapy. Based on the present study results, vincristine sulfate is still an effective monotherapy to achieve full recovery, although the number of cycles can vary, possibly depending on the expressed phenotype. Permanent loss of nasal turbinates is the sequelae of therapeutic chemotherapy. Prognosis is not correlated to the staging system but seems good with vincristine sulfate in mixed phenotype cases.

Extraction of key regions of beef cattle based on bidirectional tomographic slice features from point cloud data

As the world population grows and the increase in food consumption creates unprecedented pressure on beef producers, innovative technologies for sustainable and efficient beef production is quintessential. Therefore, a significant effort is ongoing on developing digital precision technologies for continuous beef cattle monitoring and accurate cattle growth estimation. One such technology relies on 3D visual sensor technologies. 3D point cloud data acquired with 3D cameras can be processed to evaluate the livestock’s growth condition with minimal human intervention. Essentially, the automatic measurement of animal body dimensions highly depends on the location accuracy of the key points or regions in point clouds. However, the location of specific points and regions requires extra assistance and usually leads to semi-automatic and low-accuracy solutions at best. To address these issues, we demonstrated a novel strategy for partial segmentation of beef cattle point clouds, based on the Bidirectional Tomographic Slice Segmentation (BTSS) algorithm. The cattle point clouds can be successfully segmented into the head and neck, front trunk, middle trunk, back trunk, lower leg, and hip and tail regions with high accuracies of 89%, 91%, 94%, 95%, 92%, and 95%, respectively. Consequently, the segmentation completion rate is 96%, and the average segmentation accuracy reaches 92.8%. When compared to traditional approaches, our method efficiently extracted abundant body characteristics from the beef cattle point cloud. Moreover, the proposed algorithm exhibited generalization capabilities to segment the point clouds of other cloven-hoofed livestock species. The accurate localization of key regions enabled body dimensions measurements and non-contact weighing, which can provide a solid support for breeding needs such as health evaluation, production performance measurement, and genetic breeding evaluation.

Three-year clinical outcomes and posterior corneal elevation change after small-incision lenticule extraction in suspicious corneas.

To determine the long-term clinical outcomes and change in posterior corneal elevation after small-incision lenticule extraction (SMILE) in eyes with suspicious tomographic features. Hospital clinic. Retrospective, case-controlled, observational. This study included 43 patients with suspicious corneas (group A), defined by corneal morphology and a final D score from a Scheimpflug camera (Pentacam), and 43 patients with normal corneal topography (group B). Refraction, visual acuity, and posterior corneal elevation over a 6-mm central diameter, including posterior central elevation (PCE), posterior elevation at the thinnest point (PTE), and posterior maximal elevation (PME), were measured preoperatively and at 6 months, 12 months, and 36 months postoperatively. The preoperative spherical equivalent was -5.51 ± 1.33 D in group A (n = 43) and -5.41 ± 1.19 D in group B (n = 43). Postoperative uncorrected distance visual acuity was 20/20 or better in 39 (91%) of 43 eyes in group A and 41 (95%) of 43 eyes in group B ( P = .160); all eyes in both groups remained stable or had gained corrected distance visual acuity. The mean change in PCE, PTE, and PME at 3 years was -1.22 ± 2.65 μm, -1.21 ± 2.70 μm, and -1.00 ± 5.09 μmin group A and -1.76 ± 3.25 μm, -1.60 ± 3.33 μm, and -1.56 ± 5.01 μm in group B, respectively, indicating a tendency for backward displacement of the posterior surface, whereas the between-group difference was not statistically significant ( P = .154, P = .547, and P = .319, respectively). Refraction, visual outcomes, and posterior corneal shift seem comparable between corneas with normal and suspicious tomographic features three years after SMILE. More long-term studies are warranted to corroborate the findings of this study.

Optical coherence tomography and angiography features of pigmented ocular fundus lesions in hereditary colonic polyposis syndrome.

Optical coherence tomography and angiography features of pigmented ocular fundus lesions in hereditary colonic polyposis syndrome.

Computed Tomographic Features of Canine Ovarian Masses

Computed Tomographic Features of Canine Ovarian Masses

Description de fractures avec fragments laminaires ostéochondraux inhabituels dans les fractures du radius distal

BackgroundDistal radius fracture (DRF) is one of the most common fractures and, frequently, surgical treatment is mandatory in the presence of an intra-articular fracture. However, there are some unusual intra-articular fracture patterns, were it remains challenging to properly recognize and anatomically reconstruct the articular surface. The objective of the present study is to describe an intra-articular fracture pattern of the distal radius characterized by the presence of osteochondral laminar fragments, which could potentially require a different treatment to standard stabilization. We aim to answer the following questions: (1) What are the radiological characteristics of intra-articular DRFs with osteochondral laminar fragments (OCLF), (2) What is the prevalence of DRFs with OCLF relative to all intra-articular surgical DRFs, (3) What are the differences in epidemiological characteristics of patients with OCLF in relation to all patients with intra-articular DRFs, (4) What is the prevalence of intra-articular DRFs with OCLF in patients belonging to a closed community. Patients and methodsWe reviewed radiological and tomographic records of all adult patients operated on distal radius fractures at our institution. We analyzed tomographic characteristics; prevalence of osteochondral laminar fragments relative to all intra-articular surgical distal radius fractures and compared clinical and demographic characteristics of patients with osteochondral laminar fragments in relation to all patients with intra-articular fractures. ResultsTwo main groups according to the fracture pattern were described: group I, volar rim impacted OCLF (vOCLF); and group II, central impacted OCLF (cOCLF). Prevalence of OCLF relative to surgical intra-articular DRFs: 42/989 (4.2%); group I: 23/989 (2.32%); group II: 19/989 (1.92%). Characteristics of patients with OCLF compared to all patients with intra-articular DRFs: significant differences were found in the five variables evaluated (age, less than 65 years, female, high energy fracture, and associated fractures). The global prevalence of DRFs with OCLF in patients affiliated with the medical care insurance system of our institution was 2 per 10,000 individuals (95% CI: 1.4 to 2.9). DiscussionGlobal prevalence of these fragments relative to surgical intra-articular fractures was very low. However, despite being epidemiologically rare, it is important to identify these specific fracture patterns because their treatment can be challenging. Level of evidenceIV (observational/descriptive); cross-sectional study.

CT features of confirmed and presumed gastric wall edema in dogs.

Postcontrast computed tomographic (CT) characteristics of gastrointestinal (GI) wall edema in humans have been described as GI wall thickening with a thickened submucosal layer and thin enhanced inner and outer layers. Published studies describing CT features of gastric wall edema in dogs are currently lacking. The aim of this retrospective, case series was to describe CT features of gastric wall edema in a group of dogs. Medical records were searched for dogs with postcontrast abdominal CT scans and a diagnosis of gastric wall edema based on histopathology (group I) or CT characteristics consistent with those reported in humans (group II). Clinical diagnosis, mean serum albumin concentration, and histopathological diagnosis were recorded. The following CT characteristics were recorded: numbers of wall layers, attenuation and contrast enhancement, presence of blood vessels, locations, distribution, and thickness. Twelve dogs (3 in group I and 9 in group II) were included. The most common clinical finding was hypoalbuminemia. In group I, a well-defined three-layer appearance with a non-enhancing fluid-attenuating middle layer was observed in three dogs and thin blood vessels in the middle layer in two dogs. In group II, nine dogs had a three-layer appearance with a non-enhancing fluid-attenuating middle layer. Locations of gastric wall thickening were diffuse in two, focal concentric in six, and focal asymmetric in four dogs. Findings supported including gastric wall edema as a differential diagnosis for dogs with hypoalbuminemia and CT characteristics of a three-layer appearance in the gastric wall, with a non-enhancing fluid-attenuating middle layer and thin blood vessels.

High-resolution computed tomography in the diagnosis of fibrotic hypersensitivity pneumonitis: A review

Aim. Assess the detection of rare computed tomographic features and their combination to detect cases of fibrotic hypersensitivity pneumonitis (HP). A retrospective analysis of data from high-resolution computed tomography of the lungs was performed in 52 patients with fibrotic hypersensitivity pneumonitis (fHP), in whom the clinical diagnosis was confirmed by morphological examination of lung biopsy. The analysis of the identified changes was carried out by qualitative and quantitative methods. The study included signs common to non-fibrotic and fibrotic hypersensitivity pneumonitis of HP, including ground glass symptom, mosaic density, centrilobular lesions, and emphysema. Separately, features related to pulmonary fibrosis in fHP, such as reticular changes, traction bronchiectasis, and honeycombing, were analyzed. In addition, the distribution parameters of these signs were determined separately in the cranio-caudal direction and in the axial plane. To search for combinations of tomographic features that are significant in the diagnosis, a correlation analysis of the identified changes was carried out.
 Materials and methods. The revealed CT-signs of fHP in most cases correspond to the clinical recommendations for the diagnosis of HP. However, in fHP, signs were found with a high frequency that did not correspond to the typical picture of HP, in particular, the "ground glass" symptom. On the contrary, a relatively low percentage of occurrence was observed in relation to centrilobular lesions and "mosaic density", which were also an important part of the typical HP pattern. Emphysema, which is not included in any of the HP patterns, was noted with a relatively high frequency, and in some cases was combined with the "honeycomb lung" symptom. The greatest strength of the correlation was found in such combinations of signs as "frosted glass" + reticular changes; "frosted glass" + "mosaic density"; reticular changes + "mosaic density"; emphysema + centrilobular foci, as well as reticular changes + bronchiectasis. These combinations occurred with a relatively high frequency among the examined patients.
 Results. Most of the identified changes correspond to current recommendations for the diagnosis of HP. A weak correlation between the signs does not allow us to identify combinations of signs with sufficient reliability that can help in the early diagnosis of HP.

Description of unusual osteochondral laminar fragment patterns in Distal Radius fractures

BackgroundDistal radius fracture (DRF) is one of the most common fractures and, frequently, surgical treatment is mandatory in the presence of an intra-articular fracture. However, there are some unusual intra-articular fracture patterns, were it remains challenging to properly recognize and anatomically reconstruct the articular surface. The objective of the present study is to describe an intra-articular fracture pattern of the distal radius characterized by the presence of osteochondral laminar fragments, which could potentially require a different treatment to standard stabilization. We aim to answer the following questions: (1) What are the radiological characteristics of intra-articular DRFs with osteochondral laminar fragments (OCLF), (2) What is the prevalence of DRFs with OCLF relative to all intra-articular surgical DRFs, (3) What are the differences in epidemiological characteristics of patients with OCLF in relation to all patients with intra-articular DRFs, (4) What is the prevalence of intra-articular DRFs with OCLF in patients belonging to a closed community. Patients and methodsWe reviewed radiological and tomographic records of all adult patients operated on distal radius fractures at our institution. We analyzed tomographic characteristics; prevalence of osteochondral laminar fragments relative to all intra-articular surgical distal radius fractures and compared clinical and demographic characteristics of patients with osteochondral laminar fragments in relation to all patients with intra-articular fractures. ResultsTwo main groups according to the fracture pattern were described: group I, volar rim impacted OCLF (vOCLF); and group II, central impacted OCLF (cOCLF). Prevalence of OCLF relative to surgical intra-articular DRFs: 42/989 (4.2%); group I: 23/989 (2.32%); group II: 19/989 (1.92%). Characteristics of patients with OCLF compared to all patients with intra-articular DRFs: Significant differences were found in the five variables evaluated (age, less than 65 years, female, high energy fracture, and associated fractures). The global prevalence of DRFs with OCLF in patients affiliated with the medical care insurance system of our institution was 2 per 10,000 individuals (95% CI 1.4 to 2.9). DiscussionGlobal prevalence of these fragments relative to surgical intra-articular fractures was very low. However, despite being epidemiologically rare, it is important to identify these specific fracture patterns because their treatment can be challenging. Level of EvidenceIV (Observational/Descriptive); Cross sectional study.

Tension Pneumocephalus in a Posttraumatic Patient: Computed Tomographic Findings and Case Report

Tension pneumocephalus is an unusual bad life-threatening neurosurgical emergency, this is the equivalent of tension pneumothorax. This often follows head trauma, epidural injections, complicating neurological spinal, craniofacial or sinus surgery. This is a forty-five-year-old man that was involved in a road traffic accident, was referred for a computed tomography of the brain on account of altered consciousness, irrational behavior, and restlessness. A non-contrast enhanced computed tomography of the brain was done, this demonstrated multiple areas of cortical discontinuity in the facial and skull vaults in keeping with fractures, extensive negative density (HU: -968) hypodensity in the frontal region bilaterally causing marked inferior displacement of both frontal lobes of the brain, with associated splaying of the interhemispheric fissure likened to the ‘Mount Fuji sign’ of severe/tension pneumocephalus. Pockets of negative density hypodensities are also noted in both cerebral hemispheres in keeping with pneumatoceles are also demonstrated. Generalized effacement of sulci and gyri in keeping with cerebral edema is also demonstrated. The outcome of the patient in the peripheral center was not known as at the time of this report, because all effort to get across to the patient and relations proved abortive. We present a case of tension pneumocephalus following road transport accident, to review the computed tomographic features due to its peculiar presentation.

Preoperative scale proposal based on clinical outcome for elderly patients with ruptured intracranial aneurysms undergoing microsurgery

Introduction Life expectancy in individuals has increased in recent years. There is no consensus in the literature on the best treatment for a ruptured aneurysm in the elderly (> 60 years), but some places only have microsurgery as a therapeutic strategy. This work aims to develop a prognostic scale for ruptured intracranial aneurysms in the elderly. Material and Methods Two thousand five hundred thirty patients with subarachnoid hemorrhage were retrospectively evaluated in the last ten years, and 550 of them were elderly. We developed a prognostic scale from the analysis of medical records, clinical and tomographic features that had statistical significance. Glasgow Coma Outcome (GOS) was the outcome of interest and p value < 0,05 was considered statistically significant. Results Five hundred fifty patients were evaluated, and the comorbidities that were independent variables for poor prognosis were smoking and arterial hypertension; clinical variables were Hunt-Hess, modified Rankin and Glasgow Coma Scale; tomographic was Fisher scale. Poor outcome was defined as GOS ≤ 3. Poor surgical outcomes were more remarkable in the high-risk factor categories, being 6.41 times higher among individuals who had 3 to 4 risk factors and 8.80 times higher among individuals with 5 to 6 risk factors. Conclusion In some vascular neurosurgery services worldwide, microsurgery is the only therapeutic option. This scale aimed at the elderly patient individualizes the treatment and can predict the clinical outcome in ruptured intracranial aneurysms.

KeratoScreen: Early Keratoconus Classification With Zernike Polynomial Using Deep Learning.

We aimed to investigate the usefulness of Zernike coefficients (ZCs) for distinguishing subclinical keratoconus (KC) from normal corneas and to evaluate the goodness of detection of the entire corneal topography and tomography characteristics with ZCs as a screening feature input set of artificial neural networks. This retrospective study was conducted at the Affiliated Eye Hospital of Wenzhou Medical University, China. A total of 208 patients (1040 corneal topography images) were evaluated. Data were collected between 2012 and 2018 using the Pentacam system and analyzed from February 2019 to December 2021. An artificial neural network (KeratoScreen) was trained using a data set of ZCs generated from corneal topography and tomography. Each image was previously assigned to 3 groups: normal (70 eyes; average age, 28.7 ± 2.6 years), subclinical KC (48 eyes; average age, 24.6 ± 5.7 years), and KC (90 eyes; average age, 25.9 ± 5.4 years). The data set was randomly split into 70% for training and 30% for testing. We evaluated the precision of screening symptoms and examined the discriminative capability of several combinations of the input set and nodes. The best results were achieved using ZCs generated from corneal thickness as an input parameter, determining the 3 categories of clinical classification for each subject. The sensitivity and precision rates were 93.9% and 96.1% in subclinical KC cases and 97.6% and 95.1% in KC cases, respectively. Deep learning algorithms based on ZCs could be used to screen for early KC and for other corneal ectasia during preoperative screening for corneal refractive surgery.

High Heterogeneity of Temporal Bone CT Aspects in Osteogenesis Imperfecta Is Not Linked to Hearing Loss

Objectives: To determine whether temporal bone computed tomography (CT) features are linked to the presence and type of hearing loss in osteogenesis imperfecta (OI) when considering hearing-impaired OI patients and normally hearing (NH) OI ones. A secondary objective was to assess whether other factors influence CT features in a large sample: age, type of mutation, or bone mineral density (BMD). Methods: A total of 41 adults with OI underwent CTs and pure-tone audiometry in 82 ears. Hearing thresholds were normal in 64 out of 82 ears, and most had not been operated on for stapedectomy or stapedotomy. Ossicle density, footplates, oval and round windows, retrofenestral peri- and endolabyrinths, and temporal pneumatization were analyzed twice by an experienced radiologist. CT features were compared to hearing, age, collagen mutations, and bone mineral density. Results: Unexpectedly a high prevalence of footplate, ossicle, and otic capsule anomalies was observed, even in NH ears. Footplate hypodensity or thickening was mostly found in ears without conductive hearing loss. There were significantly more retrofenestral anomalies or window obstruction in ears with a sensorineural hearing loss component than in ears without. Age was significantly higher in ears with middle layer hypodensity than in ears without. Patients with mutations were expected to have reduced collagen quantity and had significantly more footplate or retrofenestral anomalies than those with qualitative mutations. BMD was significantly higher in ears without temporal hyperpneumatization. Conclusion: Temporal bone CT features in OI are present in a large proportion of patients, had they hearing loss or not, and might be determined more by collagen mutation type than by age or BMD.

Presumed Post-COVID Infection Retinitis – Clinical and Tomographic Features of Retinitis as a Post-COVID Syndrome

ABSTRACT Purpose To study clinical and imaging features of presumed post-COVID infection retinitis. Method Retrospective case series of patients presenting with retinitis lesions with evidence of recent COVIDinfection. Retinal findings and optical coherence tomography (OCT) featureswere studied at baseline and follow-ups. Results Twenty-four eyes of 17 patients were included. Mean age was 36.57 ± 11.78 years. Baseline visualacuity (VA) was log MAR 0.97 ± 0.43. Fundus findings included retinitis patches(n = 24),hard exudates (n = 8), and superficial hemorrhages (n = 16). OCT featuresincluded neurosensory detachment (NSD, n = 20), hyperreflective inner layers (n= 24), acute macular neuroretinopathy (AMN, n = 8), hyperreflective foci (n =20). At final follow-up, VA was logMAR 0.43 ± 0.27. Retinitis patches persistedin four eyes, AMN in three eyes, and NSD in five eyes. Conclusion- Post- COVIDinfection retinitis adds to existing literature on post COVID syndromes.

Establishment and Evaluation of EGFR Mutation Prediction Model Based on Tumor Markers and CT Features in NSCLC.

Background Lung cancer has become one of the leading causes of cancer deaths worldwide. EGFR gene mutation has been reported in up to 60% of Asian populations and is currently one of the main targets for genotype-targeted therapy for NSCLC. Objective The objective is to determine if a complex model combining serum tumor makers and computed tomographic (CT) features can predict epidermal growth factor receptor (EGFR) mutation with higher accuracy. Material and Methods. Retrospective analysis of the data of patients diagnosed with in nonsmall cell lung cancer (NSCLC) by EGFR gene testing was carried out in the Department of Thoracic Surgery, Jinan Central Hospital. Multivariate logistic regression analysis was used to determine the independent predictors of EGFR mutations, and logistic regression prediction models were developed. The subject operating characteristic curve (ROC) was plotted, and the area under the curve (AUC) was calculated to assess the accuracy and clinical application of the EGFR mutation prediction model. Results Logistic regression analysis identified the predictive factors of EGFR mutation including nonsmoking, high expression level of Carcinoembryonic Antigen (CEA), low expression level of cytokeratin 19 fragments (CYFRA21-1), and subsolid density containing ground-glass opacity (GGO) component. Using the results of multivariate logistic regression analysis, we built a statistically determined clinical prediction model. The AUC of the complex prediction model increased significantly from 0.735 to 0.813 (p = 0.014) when CT features are added and from 0.612 to 0.813 (p < 0.001) when serum variables are added. When P was 0.441, the sensitivity was 86.7% and the specificity was 65.8%. Conclusion A complex model combining serum tumor makers and CT features is more accurate in predicting EGFR mutation status in NSCLC patients than using either serum variables or imaging features alone. Our finding for EGFR mutation is urgently needed and helpful in clinical practice.

Computed tomography and clinical features of Ewing's sarcoma/primary neuroectodermal tumors of the kidney: A case report and literature review

Computed tomography and clinical features of Ewing's sarcoma/primary neuroectodermal tumors of the kidney: A case report and literature review

Microbial Community Composition in Explanted Cystic Fibrosis and Control Donor Lungs

To date, investigations of the microbiota in the lungs of people with Cystic Fibrosis (PWCF) have primarily focused on microbial community composition in luminal mucus, with fewer studies observing the microbiota in tissue samples from explanted lung tissue. Here, we analysed both tissue and airway luminal mucus samples extracted from whole explanted lungs of PWCF and unused donor lungs. We determined if the lung microbiota in end-stage CF varied within and between patients, was spatially heterogeneous and related to localized structural damage. Microbial community composition was determined by Illumina MiSeq sequencing and related to the CF-Computed Tomography (CT) score and features of end-stage lung disease on micro-CT. Ninety-eight CF tissue (n=11 patients), 20 CF luminal mucus (n=8 patients) and 33 donor tissue (n=4 patients) samples were analysed. Additionally, we compared 20 paired CF tissue and luminal mucus samples that enabled a direct “geographical” comparison of the microbiota in these two niches. Significant differences in microbial communities were apparent between the 3 groups. However, overlap between the three groups, particularly between CF and donor tissue and CF tissue and CF luminal mucus was also observed. Microbial diversity was lower in CF luminal mucus compared to CF tissue, with dominance higher in luminal mucus. For both CF and donor tissue, intra- and inter-patient variability in ecological parameters was observed. No relationships were observed between ecological parameters and CF-CT score, or features of end-stage lung disease. The end-stage CF lung is characterised by a low diversity microbiota, differing within and between individuals. No clear relationship was observed between regional microbiota variation and structural lung damage.

Computed Tomographic Features of Non-Lame French Bulldog Elbows.

French Bulldogs are predisposed to humeral condylar fractures. Computed tomography (CT) in English Springer Spaniel elbows has allowed identification of humeral intracondylar fissures (HIF), which can cause lameness and predispose to condylar fractures. This study aimed to evaluate CT characteristics of non-lame French Bulldog elbows, to determine the presence of underlying elbow disease. Retrospective evaluation of CT images from elbows of non-lame French Bulldogs from scans obtained for soft tissue or neurological evaluation was performed. Images were evaluated for the presence of HIF and other elbow abnormalities: condylar and supracondylar remodelling, humeral condylar sclerosis, elbow incongruency, medial coronoid process disease and elbow osteoarthritis. Descriptive statistics were performed. Computed tomographic scans from 37 dogs (74 elbows) were reviewed. No HIF were identified, although 28/74 elbows had an intracondylar sclerotic band. Medial coronoid process disease was noted in 16/74 elbows, and small osteophytes were present in 21/74 elbows, suggesting a degree of subclinical elbow disease. Widening of the caudolateral aspect of the radial incisure was identified (37/74) as a breed normal. Elbow incongruency was otherwise rare. Non-lame French Bulldogs showed a low prevalence of occult elbow disease but a proportion had radial incisure widening. Although humeral intracondylar sclerosis was present in over a third of dogs, no HIF were identified and therefore no clear cause for their increased risk of humeral condylar fracture was identified.