Tomato Juice Research Articles

Evaluation of the germplasm collection according to directions of use in order to identify suitable genotypes for natural tomato juice

Evaluation of the germplasm collection according to directions of use in order to identify suitable genotypes for natural tomato juice

Antimicrobial properties of tomato juice and peptides against typhoidal Salmonella.

Tomatoes are readily available and affordable vegetables that offer a range of health benefits due to their bioactive molecules, such as antioxidants and antimicrobials. In contrast to the widely recognized antioxidant properties of tomatoes, their antimicrobial properties remain largely unexplored. Here, we present our findings on the antimicrobial properties of tomato juice and peptides, namely, tomato-derived antimicrobial peptides (tdAMPs), in relation to their effectiveness against typhoidal Salmonella. Our research has revealed that tomato juice demonstrates significant antimicrobial properties against Salmonella Typhi, a pathogen that specifically affects humans and is responsible for causing typhoid fever. By employing computational analysis of the tomato genome sequence, conducting molecular dynamics simulation, and performing functional analyses, we have successfully identified two tdAMPs, namely, tdAMP-1 and tdAMP-2. These tdAMPs have demonstrated potent antimicrobial properties by effectively disrupting bacterial membranes. The efficacy of tdAMP-2 is shown to be more effective than tdAMP-1. The efficacy of tdAMP-1 and tdAMP-2 has been demonstrated against drug-resistant S. Typhi, as well as hyper-capsular S. Typhi variants that possess hypervirulent characteristics, which are presently circulating in countries with endemicity. Tomato juice, along with the two tdAMPs, has demonstrated effectiveness against uropathogenic Escherichia coli as well. This underscores their potential as viable agents in combating certain Gram-negative pathogens. This study provides valuable insights into the development of effective and sustainable public health strategies that utilize tomato and its derivatives as lifestyle interventions.IMPORTANCEIn this study, we investigate the antimicrobial properties of tomato juice, the most widely consumed affordable vegetables, as well as tomato-derived antimicrobial peptides, in relation to their effectiveness against foodborne pathogens with an emphasis on Salmonella Typhi, a deadly human-specific pathogen.

Effect of Optimized UV-LED Technology on Modeling, Inactivation Kinetics and Microbiological Safety in Tomato Juice

This research analyzed, optimized and modeled the inactivation kinetics of pathogenic bacteria (PB1: Escherichia coli O157:H7 and PB2: Listeria monocytogenes) and determined the microbiological safety of tomato juice processed by UV-LED irradiation and heat treatment. UV-LED processing conditions were optimized using response surface methodology (RSM) and were 90% power intensity, 21 min and 273–275 nm (251 mJ/cm2) with R2 > 0.96. Using the optimal conditions, levels of PB1 and PB2 resulted a log reduction of 2.89 and 2.74 CFU/mL, respectively. The Weibull model was efficient for estimating the log inactivation of PB1 and PB2 (CFU/mL). The kinetic parameter δ showed that 465.2 mJ/cm2 is needed to achieve a 90% log (CFU/mL) reduction in PB1 and 511.3 mJ/cm2 for PB2. With respect to the scale parameter p > 1, there is a descending concave curve. UV-LED-treated tomato juice had an 11.4% lower Listeria monocytogenes count than heat-treated juice on day 28 (4.0 ± 0.82 °C). Therefore, UV-LED technology could be used to inactivate Escherichia coli O157:H7 and Listeria monocytogenes, preserving tomato juice for microbiological safety, but studies are required to further improve the inactivation of these pathogens and analyze other fruit and vegetable juices.

Effect of Suplementation Tomatoes Juice in Fresh Semen Egg Yolk Citrate Diluent on the Quality of Spermatozoa and the Success of Artificial Insemination in Thin Tailed Sheep

This study aims to determine the effect of adding tomato juice in fresh semen egg yolk citrate diluent to the quality of spermatozoa and the success of artificial insemination in thin tailed sheep. This research was conducted using a completely randomized design research method with 3 treatments and 5 repetitions. The treatments consisted of a solution of egg yolk citrate (P0), egg yolk citrate added 20% tomato juice (P1), and egg yolk citrate added 40% tomato juice (P2). Data analysis on spermatozoa quality included motility, viability, and abnormality using one way anova followed by Duncan’s test, while artificial insemination success included Non Return Rate (NRR) and Conception Rate (CR) using descriptive analysis. The results showed that the best diluent was found in P1 with the addition of 20% tomato juice resulting in motility percentages and viability are 75,52 ± 1,92 and 87,70 ± 1,04. It can be concluded that the addition of tomato juice had a significant (P<0,05) effect on the motility and viability of spermatozoa. The addition of 20% tomato juice in egg yolk citrate diluent can improve the quality of spermatozoa. The CR and NRR values obtained from artificial insemination with P1 diluent were 53% and 60%

Inactivation of Byssochlamys fulva during ohmic heating of tomato juice.

Ohmic heating is an emerging direct thermal technology, which uses electricity to heat food products volumetrically. Ohmic heating provides thermal and non-thermal effects like electropermeabilization to inactivate microorganisms. In this study, ohmic heating was used to inactivate Byssochlamys fulva in tomato juice. The main and interaction effects of initial pH (3.5 and 4.5) and voltage gradient (15 and 20 V/cm) were investigated on mold inactivation during ohmic heating at 88, 93, and 98 °C for 20, 10 and 5 min, respectively. The pH, acidity, total soluble solids, and Dvalue were compared. The results showed that pH and voltage gradient had significant effects on Dvalue and Zvalue (p < 0.05). In order to model the survival behavior of Byssochlamys fulva, due to the nonlinearity of the curves, Weibull model gave more accurate estimation compared to classical first-order model.

Assessing the influence of preconception diet on male fertility: a systematic scoping review.

The last decade has seen increased research on the relationship between diet and male fertility, but there are no clearly defined nutritional recommendations for men in the preconception period to support clinical fertility outcomes. The purpose of this scoping review is to examine the extent and range of research undertaken to evaluate the effect(s) of diet in the preconception period on male clinical fertility and reproductive outcomes. Four electronic databases (MEDLINE and EMBASE via Ovid, CAB Direct, and CINAHL via EBSCO) were searched from inception to July 2023 for randomized controlled trials (RCTs) and observational studies (prospective/retrospective, case-control, and cross-sectional). Intervention studies in male participants or couples aiming to achieve dietary or nutritional change, or non-intervention studies examining dietary or nutritional components (whole diets, dietary patterns, food groups or individual foods) in the preconception period were included. Controls were defined as any comparison group for RCTs, and any/no comparison for observational studies. Primary outcomes of interest included the effect(s) of male preconception diet on clinical outcomes such as conception (natural or via ART), pregnancy rates and live birth rates. Secondary outcomes included time to conception and sperm parameters. A total of 37 studies were eligible, including one RCT and 36 observational studies (prospective, cross-sectional, and case-control studies; four studies in non-ART populations) published between 2008 and 2023. Eight reported clinical outcomes, 26 reported on secondary outcomes, and three reported on both. The RCT did not assess clinical outcomes but found that tomato juice may benefit sperm motility. In observational studies, some evidence suggested that increasing fish or reducing sugar-sweetened beverages, processed meat or total fat may improve fecundability. Evidence for other clinical outcomes, such as pregnancy rates or live birth rates, showed no relationship with cereals, soy and dairy, and inconsistent relationships with consuming red meat or a 'healthy diet' pattern. For improved sperm parameters, limited evidence supported increasing fish, fats/fatty acids, carbohydrates and dairy, and reducing processed meat, while the evidence for fruits, vegetables, cereals, legumes, eggs, red meat and protein was inconsistent. Healthy diet patterns in general were shown to improve sperm health. Specific dietary recommendations for improving male fertility are precluded by the lack of reporting on clinical pregnancy outcomes, heterogeneity of the available literature and the paucity of RCTs to determine causation or to rule out reverse causation. There may be some benefit from increasing fish, adopting a healthy dietary pattern, and reducing consumption of sugar-sweetened beverages and processed meat, but it is unclear whether these benefits extend beyond sperm parameters to improve clinical fertility. More studies exploring whole diets rather than singular foods or nutritional components in the context of male fertility are encouraged, particularly by means of RCTs where feasible. Further assessment of core fertility outcomes is warranted and requires careful planning in high-quality prospective studies and RCTs. These studies can lay the groundwork for targeted dietary guidelines and enhance the prospects of successful fertility outcomes for men in the preconception period. Systematic search of preconception diet suggests that increasing fish and reducing sugary drinks, processed meats and total fat may improve male fertility, while consuming healthy diets, fish, fats/fatty acids, carbohydrates and dairy and reducing processed meat can improve sperm health.

Effectiveness of Citrullus vulgaris rubrum and Solanum lycopersicum Juices on Reducing Blood Pressure in Hypertension Patients Aged 26-45 Years

Hypertension is the second most common disease in the world, and the majority of those who suffer from it are adults. This study seeks interventions for lowering the prevalence of hypertension in the elderly and controlling blood pressure. This study compared the effects of tomato juice and watermelon juice in decreasing blood pressure in hypertensive patients at UPT Puskesmas Karang Intan 2 between the ages of 26 and 45, South Kalimantan. With pretest and post-test groups, this study used a quasi-experimental design. Thirty-two respondents were divided into intervention group 1 (watermelon juice) and intervention group 2 (tomato juice). Respondents were chosen based on research criteria and given interventions for five days. The Wilcoxon and Mann-Whitney tests were used to analyze the data. Watermelon juice and tomato juice had the same effect in lowering blood pressure in hypertensive patients aged 26 and 45 (p=0.102, =0.05). It implies that they were equally effective at lowering blood pressure because they contain the same beneficial substances in controlling blood pressure, such as potassium and citrulline.

The Effect of Additional Tomato Extract in Diluting Sodium Citrate and Egg Yellow in Kampung Chicken on The Quality of Semen Cow Limousine

Undiluted sperm will reduce fertility, therefore dilution is performed to maintain the quality of spermatozoa during storage. Tomatoes contain carbohydrates, vitamin A, vitamin C, protein, and lycopene as antioxidants. Carbohydrates and antioxidants act as a source of energy to potentially prevent free radicals that can damage cells. This study aims to determine the effect of adding tomato juice in sodium citrate diluent and free-range chicken egg yolks to the semen quality of limousin cattle. This study used a completely randomized design (CRD) with 4 treatments and 5 replications. The treatment consisted of a solution of sodium citrate and egg yolk (P0), sodium citrate and egg yolk + 10% tomato extract (P1), sodium citrate and egg yolk + 20% tomato extract (P2), and egg yolk sodium citrate + 30% tomato extract. % (P3). Parameters observed were motility, viability and abnormalities of semen spermatozoa of Limousin cattle. Observations were made at 0, 24, 48, and 72 hours after cooling. The results showed that the addition of 10% tomato extract (P1) had a significant effect on the highest percentage of motility, namely 42.00 ± 2.73c for the 48th hour, the highest viability 62.10 ± 4.51c for the 72nd hour, and lowest abnormality 16.60 ± 1.81a until the 72nd hour. The addition of 10% tomato extract (P1) in sodium citrate diluent and egg yolk resulted in the best motility, viability, and abnormality percentage of Limousin cattle spermatozoa.

Ultrasensitive electrochemical sensor via bismuth vanadate anchored graphite for detection of carbendazim in fruits and vegetables

Herein, we fabricated a novel electrochemical sensor based on bismuth vanadate/graphite nanocomposites (BiVO4/Gr/GCE) for rapid and ultrasensitive detection of carbendazim. The fabricated BiVO4/Gr/GCE sensor exhibited better electrochemical performances toward carbendazim than the sensors that modified by BiVO4, Gr and bare-electrode, respectively. For the BiVO4/Gr/GCE nano-composite, the porous Gr possesses large specific area, almighty conductivity capability, high-efficiency charge transfer, while hollow BiVO4 can promote the adsorption and electro-transfer efficiency of CBD on the BiVO4/Gr/GCE sensor. Additionally, Gr with remarkable conductivity could largely compensate the inferior-conductivity and prevent the aggregation dispersion of BiVO4. The synergistic effect of BiVO4 and Gr result in significant electro-activity good electrochemical sensing capability towards CBD. The fabricated sensor showed a wide linear response range in 4.0 × 10−8∼1.0 × 10−5, 1.0 × 10−5∼8.0 × 10−5 M with the correlation coefficient of R2 = 0.997 and R2 = 0.995, respectively. The limit of detection was 3.78 × 10−9 M (S/N = 3). Moreover, the proposed BiVO4/Gr/GCE sensor as well displays good operating stability, reproducibility, anti-disruptors, and repeatability for carbendazim detection. Furthermore, it was successfully used for carbendazim detection in pear and tomato juice with satisfactory recoveries of 90%∼108.3%, indicating that the sensor has a promising application for the detection of actual carbendazim.

Pengaruh Pemberian Jus Tomat Terhadap Tekanan Darah Pada Penderita Hipertensi di Kelurahan Mahawu Lingkungan II Kota Manado

Hypertension or high blood pressure is a condition when there is an increase in blood pressure, which is above 140 mmHg for istolic blood pressure and 90 mmHg for diastolic blood pressure.One of the therapies used to lower blood pressure is the administration of tomato juice because it is rich in lycopeine, potassium and bioflavonoids. The purpose of this study was to determine the effect of tomato juice on lowering blood pressure in hypertensive patients in Mahawu Village sub distrit II Manado City. The method used in this study is a design experiment with One Group pretest-postest design. The sample taken amounted to 15 respondents using purposive sampling. Data collection using SOPs and observation sheets. The collected data is processed using SPSS 16.0. Statistical test using Wilcoxon Test with a meaning level ( α) of 0.05. The results of the study using Wilcoxon statistical tests obtained the results of Systolic Pre-Post with a value of P value = 0.001 and Diastolic Pre-Post with a value of p Value = 0.002 where the value of p Value &lt; α = 0.05). so that Ha was accepted and Ho was rejected so that there was an effect of giving tomato juice on lowering blood pressure in people with hypertension in Mahawu Village sub district II Manado City. The conclusion is based on the results of the study that there is an effect of giving tomato juice on reducing blood pressure in hypertensive patients in Kelurahan II Manado City.

Alternaria toxins in tomato products marketed in the Russian Federation

Tomatoes and tomato products are widely produced and consumed throughout the world. Alternaria spp. are the main cause of alternariosis (black mold disease) on fresh tomatoes, both in the field and after harvesting. Alternaria toxins are widespread contaminants of tomato products. The aim of the present study was to evaluate the contamination of tomato processing products from the domestic market with Alternaria toxins, as well as to assess their intake by humans through the consumption of tomato juices. Material and methods. The content of Alternaria toxins (alternatiol, alternariol monomethyl ether, altenuene, tentoxin, tenuazonic acid) was determined in 64 samples of tomato products (paste, ketchup, juice) by high-performance liquid chromatography coupled to tandem mass-spectrometric detection (HPLC-MS/MS). Results. The priority Alternaria toxins for tomato paste, ketchup and juice were tenuazonic acid (61% of 64 samples, in amounts from 20.0 to 1065.5 μg/kg), altenuene (52%, 8.9-200.1 μg/kg) and alternariol (27%, 12.2-561.6 μg/kg). Samples of tomato paste turned out to be the most contaminated with Alternaria toxins while tomato juice samples were the least contaminated. At the same time, several toxins were found in 91% of tomato paste samples, 35% of ketchups, and 23% of tomato juices. Conclusion. To the best of our knowledge, the present study is the first survey devoted to Alternaria toxins contamination of tomato paste, ketchup and tomato juice sold on the Russian market. The high frequency of their contamination with tenuazonic acid, altenuene and, to a lesser extent, alternariol has been established, which indicates a potential risk to human health when tomato processing products are consumed. This indicates the need for a hygienic assessment of contamination the above products with tenuazonic acid, altenuene and alternariol. When calculating the potential intake of Alternaria toxins for different age population groups, it was shown that high levels of alternariol (up to 56.77 ng/kg body weight per day) could be obtained under daily consumption of tomato juice by adults and children under three years of age, as well as tenuazonic acid when consuming tomato juice contaminated at the 95th percentile level as part of the diet in organized groups for orphans and children without parental care.

A highly effective peroxidase-mimic nanozyme of S, N-carbon dot-decorated cerium organic framework-based colorimetric detection of Hg2+ ion and thiophanate methyl.

In this study, we proposed a colorimetric probe as S, N-carbon dot-decorated Ce-MOF (S, N-CD@Ce-MOF) for the dual detection of mercury and thiophanate methyl (TM), which are simultaneously present pollutants in the environment and foodstuffs. These pollutants cause serious threats to human health, such as carcinogenicity and neurovirulence. Herein, we synthesized S, N-CD@Ce-MOF using the hydrothermal method and applied it to a "turn-off-on" probe to detect mercury and TM using the colorimetric method in water and food samples. S, N-CD@Ce-MOF shows excellent peroxidase activity by catalyzing the chromogenic substrate of 3,3',5,5'-tetramethylbenzidine (TMB), resulting in deep blue-colored oxidized TMB product (ox TMB) in the presence of H2O2 with a UV absorption wavelength at 654 nm. However, the addition of Hg(II) ions prohibits the oxidation of TMB by an electron transfer effect and easily binds with -S, -N-containing sites on the surface of carbon dots, obstructing the catalytic active sites and decreasing catalytic efficiency with weak UV absorption at 654 nm as a "turn-off". Subsequently, the addition of TM to the above sensing solution as a "turn-on" was triggered by the TM-Hg complex formation and permitted TMB oxidation with a strong absorption peak at 654 nm. Furthermore, this proposed sensor demonstrates a superior linear response to mercury ions and TM in the ranges from 0 to 15 μM and 0 to 14 μM, respectively. The developed colorimetric assay exhibits good sensitivity and selectivity against various possible interferences. Furthermore, we found that the limits of detection for Hg2+ and TM were as low as 0.01 μM and 0.03 μM, respectively. The developed sensor provides various benefits, such as cost-effectiveness, simplicity without a complex detection process, and naked-eye detection. Consequently, our proposed colorimetric technique worked well for the detection of Hg2+ in real water samples and TM in real apple and tomato juice.

Effect of Tomato and Cucumber Juice on Blood Pressure in Hypertensive Patients: a Quasi-Experimental Study

Effect of Tomato and Cucumber Juice on Blood Pressure in Hypertensive Patients: a Quasi-Experimental Study

Investigation of Dielectric Properties of Potato, Tomato and Onion Juices at Microwave Frequencies

The dielectric properties of fresh juices of Solanum tuberosum (potato), Solanum lycopersicum (tomato) and Allium cepa (onion) were measured in terms of the dielectric constant and dielectric loss factor, over a frequency range from 1 to 50 GHz and at temperatures ranging from 30 to 60 °C, by using the PNA network analyzer model E8364C and open-ended coaxial probe 85070E. A prediction of relaxation frequencies of molecules for the three different juices was done and their molecular behavior was then studied by plotting dielectric constant vs. frequency and dielectric loss factor vs. frequency curves in the frequency range from 1 to 50 GHz at four different temperatures from 30 to 60 °C. The dielectric properties of food products play a crucial role in determining their heating behavior when subjected to an electromagnetic field. Understanding these properties is essential for the development of microwave pasteurization and sterilization processes tailored to various food products.

Effect of different potassium fertilizers on yield and quality of tomato (Solanum lycopersicum L.) under drought stress conditions

The experiment was carried out in open field conditions in Ciğir village, located in the Idil district of Sirnak province, during 2020 tomato growing season. The aim of the study was to investigate the effect of different potassium (potassium chloride, potassium sulfate and potassium nitrate) fertilizers on yield and quality characteristics of tomato plants (Fereng genotype and Kamenta F1 variety) grown under drought stress. The fertilizers were foliar applied at a dose of 1%. Irrigation treatments of the experiment were full irrigation (control, 100%), 66% of the full irrigation, and 33% of the full irrigation. Leaf temperature, relative water content of leaf, chlorophyll content, fresh and dry weight of green parts, membranes injury index, soluble solid content (SSC) in tomato juice, pH of tomato juice and total yield were determined. The results indicated that drought stress had a significant adverse impacts on yield and quality of both Fereng genotype and Kamenta F1 variety. The application of potassium nitrate and potassium sulfate caused an increase in the chlorophyll and water soluble solid content. Potassium chloride application resulted in a reduction in membrane damage. The effects of potassium sulfate fertilizer on yield was significantly higher than the other two potassium fertilizers.

Impact of “Golden” tomato juice on cognitive alterations in metabolic syndrome: Insights into behavioural and biochemical changes in a high-fat diet rat model

“Golden” tomato (GT) plays a protective role in metabolic dysfunction induced by High-Fat Diet (HFD). Our aim is to characterize the phytonutrient composition of the juice and explore the influence of GT, orally administered for one month, on cognitive impairment associated with Metabolic Syndrome (MetS) in male rats. We investigated reactivity, stress response and memory, together with brain neurotrophic/inflammatory signaling. Our data showed that HFD-induced functional modifications were ameliorated by GT nutritional supplementation. In particular, the behavioural reactivity improved in HFD/GT rats, that also showed a better performance in tests measuring anxiety and anhedonia. Furthermore, GT consumption rescued the declarative memory impairment. Lastly, GT supplementation counteracted HFD-induced brain alterations in PI3K\\Akt and MAPK/ERK signalling pathways. In conclusion, this study provides evidence of the importance of food supplementation with GT in the protection from neuroinflammation and cognitive alterations associated with MetS.

Pengaruh Jus Tomat Terhadap Tekanan Darah Pada Penderita Hipertensi Di Wilayah Kerja Puskesmas Passo Kecamatan Baguala Kota Ambon Provinsi Maluku

Introduction: Hypertension is a public health care that often occurs in the world and also in Indonesia or developing countries. Hypertension is also known as The Silent killer because the sufferer does not know whether he has Hypertension or not. Increased blood pressure or hypertension is caused by a chronic increase in blood pressure that occurs in the blood vessels. And if it is continuously left unchecked it will cause dysfunction in other organs, one of which is the heart and kidneys. In dealing with the problem of Hypertension they often treat Hypertension with pharmacological treatment or treatment with drugs compared to non-pharmacological treatment or without drugs. The purpose of this study was to determine the effect of tomato juice on blood pressure in Hypertensive patients. Methods: This study uses descriptive research with experimental research methods using a quasi-experimental design. The population in this study was male and 35 females. The sample in this study was 30 respondents who were hypertension sufferers. Sampling technique Based on the existing population; the determination of this study used purposive sampling. The data collection technique used in this study is to use observation sheets. Data analysis using paired t-test. Results: The results of the blood pressure study before being given tomato juice, changes in mild blood pressure were 11 people (36.7%), moderate blood pressure was 9 people (30.0%), and severe blood pressure was 10 people (33.3%). Blood pressure after being given tomato juice changes in normal blood pressure by 11 people (36.7%) mild by 18 people (60.0%) and moderate blood pressure by 1 person (3.3%). Results The statistical test results of the paired T-test for the effect of tomato juice on blood pressure in hypertensive patients obtained a p-value of 0,000 (p &lt; 0,05). Discussion: Blood pressure in hypertensive patients before being given tomato juice in the work area of ​​the Passo Health Center, Baguala District, Ambon City, Maluku Province, almost all experienced hypertension or more than normal limits. Blood pressure in hypertensive patients after being given tomato juice in the Work Area of ​​the Passo Health Center, Baguala District, Ambon City, Maluku Province, almost all respondents experienced a decrease. There is an effect of tomato juice on reducing hypertension in hypertensive patients in the Work Area of ​​the Passo Health Center, Baguala District, Ambon City.

Polysaccharides derived from Ganoderma lucidum applied to the extraction chitosan and development of electrochemical sensor based on extracted chitosan and graphitic carbon nitride modified electrode for determination nitrite in food samples

The current study focused on extracting chitosan from polysaccharides generated from Ganoderma lucidum and applying it to the construction of electrochemically modified electrodes based on graphitic carbon nitride (g-C3N4) for the measurement of nitrite in water and food samples. Ganoderma Lucidum polysaccharides were isolated and characterized. The chitosan was then isolated enzymatically from the polysaccharides using a chemical technique. The isolated chitosan and g-C3N4 were used to modify a GCE (chitosan/g-C3N4/GCE). The structural investigations employing FT-IR, SEM, and XRD show that chitosan successfully interacted with g-C3N4 in the chitosan/g-C3N4 sample. Electrochemical evaluation using cycle voltammetry (CV), differential pulse voltammetry (DPV), and amperometry revealed that the chitosan/g-C3N4/GCE electrode has good sensitivity and selectivity for determining nitrite. The use of chitosan and g-C3N4 in electrode surface modification improved nitrite electro-oxidation performance by allowing for more effective adsorption and electron transport processes. DPV measurement showed that the sensor had a sensitivity of 0.12103 μA/μM, a linear range of 40 to 2000 μM, and a limit of detection of 21 nM, and amperometry measurement showed the sensor's sensitivity of 0.15487 μA/μM, linear range of 20 to 4230 μM, and an LOD of 10 nM. These findings indicated a more sensitive and wide range of sensor performance using amperometry with respect to the DPV technique. The method's validity and accuracy in determining nitrite in prepared genuine samples (water, milk, apple juice, orange juice, Aloe vera, and tomato juice) were investigated. RSD values (3.19 % to 4.75 %) and recovery values (80.00 % to 98.33 %) were found to be satisfactory. The data also revealed that there were no substantial matrix interferences, demonstrating the accuracy and applicability of the proposed approach for detecting nitrite in food samples. Therefore, the proposed sensor design has several advantages that make it a promising candidate for various applications. Its reusability, stability, wide linear range, accuracy, and low cost make it an attractive option for many industries.

A new spiral press filter to purify tomato juice: A central composite strategy for experimental design and optimization

We investigated and optimized the simultaneous effects of pressure and fluid temperature on the tomato juice filtration process. For this purpose, we manufactured a new spiral press filter. This filter is innovative in that it has a fixed spiral cylinder, while the fluid travels along the spiral path using hydraulic force. This design has the advantages of both press filters and spiral press filters. The turbidity of filtered tomato juice decreased from 1632NTU to 643NTU. The optimum conditions for the tomato juice filtration process were found to be 6 bar fluid pressure and 45 °C fluid temperature. These conditions resulted in a desirability index of 0.99, which is a very good value. The optimum values of the response variables, including permeate flux, total energy consumption, lycopene content, and vitamin C content of filtered tomato juice were 503.15 × 10−6 m3 m2 s−1, 0.033 kWh, 2.44 mg 100 g−1, and 13.40 mg 100 g−1, respectively.

Abstract 17418: Daily Intake of Fruits and Vegetables Moderates the Effect of a Nutrition Intervention on Symptom Burden in Patients With Heart Failure

Introduction: Patients with heart failure (HF) often remain symptomatic after optimization of medical treatment. Complementary interventions may reduce symptom burden. We conducted a 6-month randomized controlled trial of a nutrition intervention (NIHFT) to reduce fluid overload, oxidative stress, and inflammation, which are major pathologic pathways underlying HF symptoms.Aims: 1) To examine the effects of NIHFT on HF symptom burden (i.e., shortness of breath, edema, and fatigue); and 2) to determine if intake of fruits and vegetables, primary sources of antioxidants and anti-inflammatories, moderated the effect of the NIHFT on HF symptom burden. Methods: Patients with HF (n=116) with complete baseline data were included (61% male, 63±12 years old, 64% NYHA III/IV). Intervention group received 1) individualized educational intervention for sodium reduction that included promoting fruit and vegetable intake, 2) daily low sodium tomato or vegetable juice, and 3) omega-3 supplementation (1050 mg EPA, 150 mg DHA/capsule/day) for 6 months. Symptom burden was measured by the Memorial Symptom Assessment Scale-HF at baseline and 3 and 6-month. Daily intake of fruits and vegetables at baseline was dichotomized (≤1 vs ≥2 servings/day). A linear mixed-effect model was used. Results: There was no direct effect of the NIHFT on symptom burden. However, baseline intake of fruits and vegetables moderated the effect of the intervention on overall symptom burden scores (beta estimate= -4.67, SE=1.89, P=0.015). Reduction in symptom burden was greater among patients with lower baseline fruit and vegetable intake compared to those with higher intake. Among the subscales (i.e., frequency, severity, and distress) of overall symptom burden, frequency (beta estimate= -1.63, SE=0.66, P=0.014) and severity (beta estimate= -1.81, SE=0.61, P=0.004) were significantly moderated by the intake of fruits and vegetables. Conclusions: The findings indicate that the nutrition intervention was more effective in reducing symptom burden for patients with lower baseline intake of fruits and vegetables. This suggests that increased fruit and vegetable intake may further reduce HF symptom burden in patients on optimal medication therapy.