Undeviating climatic instabilities have increased the incidents of drought. Crop performance and yield attributes of tomatoes are negatively affected by drought stress. Biochar is an organic amendment that can increase crop yield and nutritional value under water-deficient conditions by retaining water and providing nutrients (nitrogen, phosphorus, potassium, and other trace elements). The present study was designed to investigate the effects of biochar on tomato plant physiology, yield, and nutritional quality under deficit moisture regimes. Plants were subjected to two biochar levels (0.1% and 0.2%) and four moisture levels [100%, 70%, 60%, and 50% field capacities (FCs)]. Drought stress, especially 50D (50% FC), severely affected the plant morphology, physiology, yield, and fruit quality attributes. However, plants grown in biochar-amended soil showed significant increase in the studied attributes. Plant height, root length, fresh and dry weight of root, the number of fruits per plant, fruit fresh and dry weight, ash percent, crude fat, crude fiber, crude protein, and lycopene contents were increased in plants grown in biochar-amended soil under control and drought stress. Biochar at 0.2% application rate depicted a more pronounced increment in the studied parameters than 0.1% and can save 30% water without compromising tomato crop yield and nutritional value. © 2023 Society of Chemical Industry.