Tolerant Scheme Research Articles

Heat and drought priming induce tolerance to subsequent heat and drought stress by regulating leaf photosynthesis, root morphology, and antioxidant defense in maize seedlings

Novel stress tolerance strategies, such as heat and drought priming, are widely used in plants to increase tolerance to subsequent stresses. To date, it is still not known whether heat, drought, and combined stress priming can improve the tolerance of maize plants to heat, drought, and combined stresses by improving the morphology and distribution of roots and antioxidant defense mechanisms in maize seedlings. Here, seedlings of maize were firstly twice exposed to heat, drought, and combined stress at 33 °C/25 °C and 50–60% of soil water holding capacity (SWHC) followed by temperature and water recovery, and then subjected to more severe heat, drought, and combined stress at 36 °C/28 °C and 45–55% of SWHC. Under individual heat or drought stress, healthy values of gas exchange and chlorophyll fluorescence parameters, leaf relative water content, leaf water potential, and leaf cooling were significantly reduced, while superoxide anion and MDA contents in roots were significantly increased. Combined heat and drought stress usually exhibit a typical superimposed effect for the abovementioned parameters. Plants with heat, drought, and combined stress priming improved the redox balance of maize roots by enhancing the activities of superoxide dismutase, peroxidase, and catalase, initiated increases in root vitality as well as osmoregulatory substance contents as evidenced by increased soluble sugar, soluble protein, and proline contents. Principal component analysis revealed a strong correlation between antioxidant enzyme activities and osmoregulatory substance contents in roots. The combined stress priming effectively increased total root length, total root surface area, total root volume, and total root dry weight when compared with no priming. Combined stress priming optimized root distribution, leading to an increase in root length density and root dry weight density in the 12–18, 18–24, and 24–30 cm soil layers. Additionally, a strong positive correlation was observed between leaf relative water content, leaf water potential, and leaf cooling, indicating that better leaf water relations in the primed plants were conducive to the improvement of leaf cooling capacity, which provides good conditions for the increase in photosynthetic rate to maintain the integrity of the maize plant. The significant increases in leaf area, specific leaf weight, aboveground dry mass, and a significant reduction in leaf rolling in combined stress-primed plants indicated that the negative effects of heat and drought stress can be effectively mitigated. The current data indicated that the beneficial stress memory induced by priming in maize seedlings developed their defense systems to trigger more effective clearance mechanisms against subsequent combined heat and drought stress. These findings provided evidence that heat, drought, and combined stress priming enhanced maize adaptability to subsequent heat, drought, and combined stress by improving the morphology, distribution, and antioxidant capacity of the root system. • The effects of combined heat and drought stress on leaf water relations, photosynthesis, , and root physiological activities were studied. • Combined stress-primed plants acquired a stress imprint. • The enhanced tolerance of primed plants to heat and drought stress may be attributed to improved physiological activities in roots.

Fuzzy model based fault estimation and fault tolerant control for flexible spacecraft with unmeasurable vibration modes

This paper investigates the problem of anti-disturbance fault estimation and attitude tracking fault tolerant control for a flexible spacecraft subject to actuator faults, external disturbances, input saturation, and configuration misalignment. Different form the traditional methods, both the nonlinear dynamics subsystem and attitude control system are reconstructed into Takagi–Sugeno fuzzy models, where partial nonlinear term is remained to assist the design of fuzzy controller. A fuzzy fault estimation observer with proportional–integral adaptive laws is first designed. Without measuring any dynamic information of flexible appendages, estimated values of unknown actuator faults and vibration disturbances of flexible appendages can be obtained simultaneously. This is the most important advantage of the proposed fault estimation scheme over the previous methods. Then, a fuzzy sliding mode fault tolerant control scheme, based on fewer fuzzy rules and less computational burden, is developed under the framework of feedback linearisation and feedforward compensation. The actuator faults and vibration disturbances are counteracted in real time. Regional pole placement and virtual controller with input saturation are adopted to tune the control input and avoid the radical control torque scheduling. Finally, numerical simulations are given to illustrate the validity of the proposed fault estimation and fault tolerant control strategies.

Different Drought Tolerance Strategy of Wheat Varieties in Spike Architecture

Spike characteristics include spike length, total spikelet number per spike, number of fertile flowers, spike density, spike fertility, grain number, thousand kernel weight, the number of spikes per square meter, harvest index and the grain yield during the flowering and ripening stage. The six winter and one facultative variety differed in earliness, derived in part from the allele of the Ppd-D1 gene and phenological observation. The two sites significantly differed in the soil moisture, which varied during continual microclimate monitoring. The spike architecture of winter wheat was affected by drought. The plant samples from the site FIELD 2 (more drought stressed) showed a higher reduction in spike characteristics such as a lower spike length, total spikelet number, number of fertile flowers and spike fertility, leading to a lower yield than the site FIELD 1. Both early and late varieties possess compensatory abilities to create the grain yield during drought stress; however, the timing and duration of exposure to drought determine the application and success of the compensatory ability. In our experiment, the late varieties (photoperiod sensitive) performed better in yield than the early varieties during both growing seasons. That is at odds with the generally recommended “drought escape strategy” (early varieties) and suggests a possible direction for variety selection and breeding in arid areas in Central Europe.

Discovery of a new genus of anaerobic ammonium oxidizing bacteria with a mechanism for oxygen tolerance

In the past 20 years, there has been a major stride in understanding the core mechanism of anaerobic ammonium-oxidizing (anammox) bacteria, but there are still several discussion points on their survival strategies. Here, we discovered a new genus of anammox bacteria in a full-scale wastewater-treating biofilm system, tentatively named “Candidatus Loosdrechtia aerotolerans”. Next to genes of all core anammox metabolisms, it encoded and transcribed genes involved in the dissimilatory nitrate reduction to ammonium (DNRA), which coupled to oxidation of small organic acids, could be used to replenish ammonium and sustain their metabolism. Surprisingly, it uniquely harbored a new ferredoxin-dependent nitrate reductase, which has not yet been found in any other anammox genome and might confer a selective advantage to it in nitrate assimilation. Similar to many other microorganisms, superoxide dismutase and catalase related to oxidative stress resistance were encoded and transcribed by “Ca. Loosdrechtia aerotolerans”. Interestingly, bilirubin oxidase (BOD), likely involved in oxygen resistance of anammox bacteria under fluctuating oxygen concentrations, was identified in “Ca. Loosdrechtia aerotolerans” and four Ca. Brocadia genomes, and its activity was demonstrated using purified heterologously expressed proteins. A following survey of oxygen-active proteins in anammox bacteria revealed the presence of other previously undetected oxygen defense systems. The novel cbb3-type cytochrome c oxidase and bifunctional catalase-peroxidase may confer a selective advantage to Ca. Kuenenia and Ca. Scalindua that face frequent changes in oxygen concentrations. The discovery of this new genus significantly broadens our understanding of the ecophysiology of anammox bacteria. Furthermore, the diverse oxygen tolerance strategies employed by distinct anammox bacteria advance our understanding of their niche adaptability and provide valuable insight for the operation of anammox-based wastewater treatment systems.

Risk reduction for safety of the intended functionality of CACC with complex uncertainties: A cooperative robust non-fragile fault tolerant strategy

Safety of the intended functionality (SOTIF) of connected and autonomous vehicles (CAVs) is very challenging due to heterogeneous performance limitations and complex uncertainties in real road environments. In this paper, a cooperative robust non-fragile fault tolerant control (CRNFTC) strategy is presented to reduce the risks caused by multi-vehicle performance limitations of perception and actuation systems for ensuring SOTIF of cooperative adaptive cruise control (CACC). First, an intermediate based cooperative robust estimation method is proposed, by combining ego and cooperative information, system states, matched disturbances, performance limitations of perception and actuation of the vehicles in the platoon are simultaneously estimated. Existence conditions of estimators are given with respect to predecessor following (PF) and bi-directional (BD) communication network topologies. Then, based on the estimation, a distributed robust non-fragile fault tolerant H∞ control method is proposed, such that compensation of the effects of heterogeneous performance limitations and matched disturbances, and suppression of the remaining unmatched disturbances can be achieved. The sufficient condition and optimal solution of the proposed CRNFTC are obtained by using Lyapunov theory with explicit consideration of the system uncertainty and controller perturbation. The efficacy and superiority of the proposed CRNFTC for ensuring SOTIF of the CACC of PF and BD communication network topologies with complex uncertainties are substantiated via a series of simulations and quantitative comparisons.

Recent developments in multi-omics and breeding strategies for abiotic stress tolerance in maize (Zea mays L.).

High-throughput sequencing technologies (HSTs) have revolutionized crop breeding. The advent of these technologies has enabled the identification of beneficial quantitative trait loci (QTL), genes, and alleles for crop improvement. Climate change have made a significant effect on the global maize yield. To date, the well-known omic approaches such as genomics, transcriptomics, proteomics, and metabolomics are being incorporated in maize breeding studies. These approaches have identified novel biological markers that are being utilized for maize improvement against various abiotic stresses. This review discusses the current information on the morpho-physiological and molecular mechanism of abiotic stress tolerance in maize. The utilization of omics approaches to improve abiotic stress tolerance in maize is highlighted. As compared to single approach, the integration of multi-omics offers a great potential in addressing the challenges of abiotic stresses of maize productivity.

Genetic variation and molecular characterization of Zygophyllum coccineum L. ecotypes of the iron mining area of El-Wahat El-Bahariya in Egypt

Remediation and mitigation processes can recover the ecosystems affected by mining operations. Zygophyllum coccineum L. is a native indigenous xerophyte that grows in Egypt's Western Desert, particularly around the iron mining ore deposits, and accumulates high rates of potentially toxic elements (PTEs) in its succulent leaves. The present study evaluated the genetic variation and molecular responses of Z. coccineum to heavy metal stressful conditions in three sites. Results revealed that Z. coccineum bioaccumulation capacity was greater than unity and varied amongst the three locations. In response to heavy metal toxicity, Z. coccineum plants boosted their antioxidative enzymes activity and glutathione levels as a tolerance strategy. Anatomically, a compact epidermis, a thick spongy mesophyll with water storage cells, and a thicker vascular system were observed. Protein electrophoretic analysis yielded 20 fragments with a polymorphism rate of 85%. The antioxidant genes (CAT: catalase, POD: peroxidase and GST: polyphenol oxidase) showed greater levels of expression. In addition, DNA-based molecular genetic diversity analyses using Start Codon Targeted (SCoT) and Inter Simple Sequence Repeat (ISSR) markers yielded 54 amplified fragments (i.e. 24 monomorphic and 30 polymorphic), with 12 unique fragments and a polymorphism rate of 55.5%. The greatest PIC values were recorded for SCoT-6 (0.36) and for both of the 14 A and 44 B ISSR primers (0.25). Diversity index (DI) of all SCoT and ISSR amplified primers was 0.23. The present findings reveal the distinct heavy metal's adaption attributes of Z. coccineum, indicating its improved survival in severely arid mining environments.

Abstract A036: Surfaceome profiling of rhabdomyosarcoma reveals B7-H3 as a mediator of immune evasion

Abstract Introduction: Pediatric rhabdomyosarcoma (RMS) is the most common soft tissue tumor in children, with nearly 20% of children presenting with locally aggressive and/or metastatic disease. Despite aggressive chemotherapy and radiotherapy, the 5-year overall survival rate is still less than 40%. In addition, resistance to chemotherapy remains the major cause of cancer-related mortality. Therefore, there is an unmet need to develop more effective and tolerable therapeutic strategies for the treatment of RMS. Our overall objective is to leverage transcriptomic and proteomic profiling methods to identify RMS-specific surface proteins targetable with immune-based therapies. Methods: To identify therapeutic targets, we performed cell-surface capture and mass spectrometry on fusion-positive (FP), fusion-negative (FN) RMS and normal skeletal muscle. Transcriptomic datasets of RMS tumor specimens and normal tissue were employed to identify high-confidence RMS-specific cell-surface proteins. Expression of immune checkpoint molecules was confirmed by flow cytometry, western blot and PCR. Paraffin-embedded tissue sections were used to determine protein expression and prognostic value in RMS patients. We developed an in vitro T-cell killing assay to assess the functional role of immune checkpoint molecules on the antitumor immune response. Results: Surfaceome profiling of RMS cells revealed unique protein signatures specific for each tumor type and normal tissue. We have identified 5061 cell-surface proteins in RMS and 31.6% of proteins were correctly located at the plasma membrane. By integrating proteomic and transcriptomic data, we have identified 30 and 38 proteins significantly overexpressed in fusion-negative and fusion-positive RMS respectively compared to normal muscle. We have identified the immune checkpoint molecule B7-H3 as cell-surface protein overexpressed in both RMS subtypes. Strong expression of B7-H3 was also observed in patient’s tumor specimens. Genetic deletion of B7-H3 gene in RMS cells was associated with higher antitumor immune response in tumor cells co-cultured with CD8+-T cells. No difference was observed in tumor cell proliferation and radioresistance. Conclusions: Through integrated transcriptomic and proteomic profiling, we have discovered B7-H3 as a key regulator of tumor immune-evasion in RMS. B7-H3 is a suitable immunotherapy target for the treatment of RMS. Further studies are warranted to elucidate underlying mechanisms of B7-H3 function in RMS. Citation Format: Roxane R. Lavoie, Fabrice Lucien, Patricio C. Gargollo, Mohamed E. Ahmed, Yohan Kim, Emily Baer, Doris Phelps, Cristine M. Charlesworth, Benjamin J. Madden, Liguo Wang, Peter J. Hougthon, John Cheville, Haidong Dong, Candace F. Granberg. Surfaceome profiling of rhabdomyosarcoma reveals B7-H3 as a mediator of immune evasion [abstract]. In: Proceedings of the AACR Special Conference: Sarcomas; 2022 May 9-12; Montreal, QC, Canada. Philadelphia (PA): AACR; Clin Cancer Res 2022;28(18_Suppl):Abstract nr A036.

Clinical trials for renal allograft tolerance induction through combined hematopoietic stem cell transplantation: A narrative review.

During the last four decades, development of effective immunosuppressants has significantly improved short-term results of organ transplantation. However, long-term results have not been satisfactory due to chronic rejection or complications caused by immunosuppressive drugs. Therefore, induction of immunological tolerance of the transplanted organ is considered essential to improve the long-term results. Despite numerous tolerance strategies that have been successful in murine models, inducing hematopoietic chimerism through donor hematopoietic stem cell transplantation is the only method that reproducibly induces kidney allograft tolerance in nonhuman primates or humans. Combining kidney and hematopoietic stem cell transplantation to achieve allograft tolerance has now been attempted with different chimerism strategies. This review summarizes the status of current clinical trials on the induction of allograft tolerance. We also summarize recent studies to extend the chimerism approach to deceased donor transplant recipients.

Complete remission of alpha-fetoprotein-producing gastric cancer by combined tislelizumab-apatinib treatment of a patient with proficient mismatch repair: a case report

BackgroundAlpha‑fetoprotein-producing gastric cancer (AFPGC) is a rare type of gastric cancer with a high rate of metastasis and poor prognosis. Despite substantial progress in the treatment of many solid tumors, there are no reports of the safety and effectiveness of immune checkpoint inhibitors in combination with antiangiogenesis agents for AFPGC patients who have proficient mismatch repair.Case presentationWe describe a 69-year-old man who was diagnosed with metastatic AFPGC. After progression to chemotherapy resistance, tislelizumab combined with apatinib was administered, although the patient’s gastroscopic pathology showed proficient mismatch repair. After three cycles of therapy, partial remission (reduced by 56%) was obtained, and the quality of life improved significantly. Surprisingly, after more than 1 year of continuous application of the combination treatment regimen, both the primary and metastatic tumors in this patient eventually disappeared, which obtained complete remission without surgery. The patient has had a progression-free survival of more than 24 months and is still continuing to benefit.ConclusionsThis case is the first example of effective treatment of AFPGC with tislelizumab combined with apatinib. The outcomes of this case suggest a highly effective and tolerable therapeutic strategy for microsatellite-stabilized AFPGC.

Adaptation des génotypes de luzerne pérenne à l’environnement hors oasien en vue d’améliorer les performances de l’agro-système du sud Tunisien

La sélection de la luzerne dans des zones à fortes contraintes abiotiques a peu progressé dans l’amélioration de la productivité et de la tolérance. La difficulté réside dans l’identification et la caractérisation des paramètres liés à la résistance aux différentes pressions climatiques. Divers processus phénologiques, morphologiques, physiologiques et chimiques sont à l’origine des stratégies d’évitement et de tolérance. Le but de ce travail était d’étudier les divergences morpho-physiologiques et chimiques entre huit génotypes de luzerne pérenne d’origines différentes : 3 génotypes locaux (IRA, Gabès, Chenini) et cinq exotiques (Ameristand, Bami, Prosementi, Tamentit et Tata) ayant montré une grande tolérance au bioclimat aride en dehors de l’oasis. Les résultats obtenus ont permis d’identifier deux groupes de génotypes suffisamment homogènes qui pourraient être considérés comme un patrimoine génétique intéressant pour les critères de sélection choisis. Ce pool pourra être le matériel génétique de départ pour la création de variétés synthétiques adaptées aux conditions arides en dehors des oasis caractérisées par des rendements frais et secs plus élevés (Ameristand, Bami, et Tamentit), avec une excellente tolérance à la salinité (Gabès et IRA).

POS-123 EXTRACORPOREAL PHOTOPHERESIS AS A WAY TO BALANCE BETWEEN ACUTE RENAL ALLOGRAFT REJECTION AND INFECTIVE COMPLICATIONS

Infectious complications are the second cause of death in kidney transplant recipients [Gamal M. et al., 2022; Agrawal A. et al, 2022; van Delden C. et al., 2020; AwanAA et al., 2018]. The results of kidney transplantation (KT) in recent days are associated with individualized immunosuppression strategies of immune tolerance to the graft [BauerAC et al., 2020]. Extracorporeal photopheresis (ECP) repeatedly proved its efficiency for the prevention and treatment of acute renal gfaft rejection with a decrease in complications associated with immunosuppression [Xipell M.

Open-Circuit Fault-Tolerant Control and Topology Switching Strategy of Active Magnetic Bearing Power Electronics Controller

Active magnetic bearing (AMB) technology has been widely used in high-speed electrical machines. Power electronics controller amplifier is an important segment in AMB system. Failure of power electronics device is a common threat to the reliability of AMB amplifier and can cause serious damage. In order to improve the robustness of the amplifier, this article proposes open-circuit fault-tolerant control strategies based on different topologies. For single-axis systems, a pair of windings with a single degree of freedom can work in four different working modes based on the three-phase full-bridge structure. It can detect and locate the open-circuit fault of a power electronics switch in operation. After promotion to multiaxis systems, a novel kind of topology for amplifier with fault tolerant strategy has been proposed that can also detect the open-circuit fault of power electronics switch and switch to fault tolerance mode for a multiaxis system. Fault tolerant experiments verify the feasibility and rapid response of different fault tolerance strategies with an AMB testbed in this article.

Impacts of dosing and drug withdrawal period on tacrolimus-based triple therapy in a non-human primate renal transplantation model

Non-human primate (NHP) renal transplantation models are widely used vivo models for researching new immunosuppressive therapies including allograft tolerance strategies. To enroll animals into a tolerance study, an immunosuppressive regimen that efficiently establishes stable renal function in NHPs is needed. Here, we assessed the effect of triple therapy comprising 2.0 mg/kg tacrolimus, mycophenolate mofetil and a steroid and its success rate for achieving stable renal function. In addition, to predict the pathophysiological consequences of withdrawing immunosuppressants, an indispensable process after induction of tolerance, we also assessed changes in the stable renal state maintained by triple therapy after drug withdrawal. Six cynomolgus monkeys were used. The median survival time was >176 days over the dosing period and 45 days after drug withdrawal. The triple therapy successfully induced stable graft function without calcineurin inhibitor nephrotoxicity in three of six recipients, although adopting trough-dependent tacrolimus dose adjustment rather than a preset dose regimen could improve on the present strategy. Further, drug withdrawal led to deterioration of renal function, de novo donor specific antibody production and increased the memory/naïve T cell ratio within two weeks post drug withdrawal. We expect that these findings contribute to establish one of the choices for animal model for evaluating future tolerance therapy for renal transplantation.

Efficacy of Combination Therapy with Lenvatinib and Radioactive Iodine in Thyroid Cancer Preclinical Model

Patients with differentiated thyroid cancer (DTC) usually have good prognosis, while those with advanced disease have poor clinical outcomes. This study aimed to investigate the antitumor effects of combination therapy with lenvatinib and 131I (CTLI) using three different types of DTC cell lines with different profiling of sodium iodide symporter (NIS) status. The radioiodine accumulation study revealed a significantly increased radioiodine uptake in K1-NIS cells after lenvatinib treatment, while there was almost no uptake in K1 and FTC-133 cells. However, lenvatinib administration before radioiodine treatment decreased radioiodine uptake of K1-NIS xenograft tumor in the in vivo imaging study. CTLI synergistically inhibited colony formation and DTC cell migration, especially in K1-NIS cells. Finally, 131I treatment followed by lenvatinib administration significantly inhibited tumor growth of the NIS-expressing thyroid cancer xenograft model. These results provide important clinical implications for the combined therapy that lenvatinib should be administered after 131I treatment to maximize the treatment efficacy. Our synergistic treatment effects by CTLI suggested its effectiveness for RAI-avid thyroid cancer, which retains NIS function. This potential combination therapy suggests a powerful and tolerable new therapeutic strategy for advanced thyroid cancer.

Colonization and phytoremediation potential for Miscanthus sacchariflorus in copper tailings

ABSTRACT A pot experiment was conducted to explore the effects of copper (Cu) tailings with various proportions in the substrate on seed germination and morphological traits of the plant. Concurrently, to identify the adaptive and tolerance strategies of the plant to Cu tailings, the uptake and accumulation of the plant to heavy metals, variations in soil enzymatic activities, and metal speciation in the blank, rhizospheric, and non-rhizospheric soils were estimated. Cu tailings at 25% proportion in the substrate exerted no significant negative effects on seed germination and seedling growth. However, Cu tailings at higher proportions (≧50%) inhibited seed germination and disturbed the plant physiological metabolism and growth. More biomass allocated to the plant roots could contribute to more heavy metals being immobilized, arresting their translocation from roots to shoots. This was accepted as a crucial defense mechanism for the plant against heavy metal co-contamination. The plant can improve the biological properties of Cu tailings in terms of enhanced invertase and phosphatase activities. And in turn, this can effectively alleviate heavy metal phytotoxicity. Simultaneously, it markedly decreased exchangeable Cu and Zn content in the rhizosphere in 25% Cu tailings treatments. In 50% Cu tailings treatment, no differences were observed in Zn speciation between rhizosphere and non-rhizosphere soils. In 75% Cu tailings, compared to the non-rhizosphere, an obvious reduction in exchangeable Cu in rhizosphere occurred, while an opposite tendency was demonstrated in carbonate-bound Zn. The plant could successfully colonize in Cu tailings, and represent a phytoremediation potential in Cu tailings.

Demonstration of a fault impact reduction control module for wind turbines

Abstract. Traditionally, wind turbines in distributed applications make control decisions as isolated systems. They generally provide maximum power output during operation and manage internal faults with little consideration of the rest of the power system. Although fault detection and tolerance schemes are widely researched and implemented, controls to ameliorate such faults are uncommon in research and industry. The rapid shutdown of a wind turbine in a large transmission-connected wind plant will have a minimal impact on a large power system, but in a microgrid or isolated grid context the abrupt loss of a single wind turbine may cause grid instability and high stress on the system. This paper demonstrates a fault impact reduction control (FIRC) module for a wind turbine, which implements wider warning thresholds around fault thresholds. When the turbine crosses a warning threshold, the controller sends its predicted action to the grid controller, which facilitates the grid operator’s response to a potential wind turbine fault and then takes appropriate action to ameliorate the fault. Various test cases demonstrate the controller action under a variety of faults, and various scenarios demonstrate the grid benefit of an FIRC in both microgrid- and grid-connected contexts. The FIRC maximizes wind turbine generation and eases generation transition under a variety of fault scenarios. The FIRC module is easy to integrate with most existing controllers, just requiring derate capability, and can be easily modified to include the various warnings and thresholds that the user desires. This analysis is mainly performed in a MATLAB-Simulink-based research wind turbine model and is also implemented in the existing LabVIEW-based controller of the same research turbine at NREL.

Options to the Use of Suspension and Expulsion in Kenyan Institutions: A Literature Review

This article compared the effectiveness of punitive strategies such as suspension and expulsion to mitigate behaviour disorders in Kenyan schools. Behaviour disorders witnessed in Kenyan schools include externalising and internalising behaviour disorders. Externalising behaviour disorders include bullying, aggression, disruption, acting out, fighting, violence, destruction of property, cheating, and stealing among others. Internalising behaviour disorders include depression, anxiety, social withdrawal, immaturity, negative talk, substance abuse, loneliness or guilt, sadness, suicidal feelings, nervousness, and irritability among others. Suspension and expulsion are the zero tolerance (ZT) strategies used in our institutions to curb behaviour disorders. Zero tolerance measures are guidelines for providing the harshest retribution possible to every student who goes against the laid down regulations. Corporal punishment is also used as a punitive measure in our institutions despite its prohibition in the Children’s Act 2001. Corporal punishment was recognised to offer minor data on appropriate action to take but only educate the person on the inappropriate behaviours to avoid. This article found that solitary disciplinary practices like suspension and expulsion may further prohibit poor academic performance and dropout. The article further found out the importance of training the heads of learning institutions on how to manage inappropriate behaviour disorders and the incorporation of other strategies such as guidance and counselling and the involvement of parents. Thus, it encourages Kenyan institutions to adopt School-Wide Positive Behavioural Interventions and Supports (SWPBIS), which is a non-condoning approach used in controlling school violence and misbehaviour of students that have shown positive results in an unsystematically controlled trial research to control school violence and misbehaviour of students. This article also found out the need for a Functional Behavioural Assessment (FBA) before intervening for a behaviour disorder. The aim of an FBA is to collect wide and particular data so as to comprehend the exact purpose of the student’s inappropriate behaviours.

A REVIEW ON OPTIMIZATION TECHNIQUE FOR FAULT TOLERANCE IN CLOUD COMPUTING

It is the outcome of the emergence of on demand service in big scale distributed computing models. Adaptable technology because it delivers software and resources that can be flexibly scaled up and down. These systems have varying degrees of reliability. Tolerance to unexpected hardware or software failures is measured by a system's fault tolerance. As a result of cloud computing’s inability to handle a wide range of errors, its reputation has been tarnished. Tolerating Byzantine errors is difficult since they typically go unnoticed at the beginning and can quickly spread to other virtual machines before being discovered. Thus, vital applications such as air traffic control, online baking, and so on are still avoiding the cloud because of these concerns. As well as some existing models, the goal of this research is to better understand and compare fault tolerance strategies utilised for fault tolerance in cloud systems. The purpose of this work is to evaluate a cloud -based fault-optimization strategy that will address the shortcomings of existing algorithms.

Functional Analysis of Conserved Hypothetical Proteins from the Antarctic Bacterium, Pedobacter cryoconitis Strain BG5 Reveals Protein Cold Adaptation and Thermal Tolerance Strategies.

Pedobacter cryoconitis BG5 is an obligate psychrophilic bacterium that was first isolated on King George Island, Antarctica. Over the last 50 years, the West Antarctic, including King George Island, has been one of the most rapidly warming places on Earth, hence making it an excellent area to measure the resilience of living species in warmed areas exposed to the constantly changing environment due to climate change. This bacterium encodes a genome of approximately 5694 protein-coding genes. However, 35% of the gene models for this species are found to be hypothetical proteins (HP). In this study, three conserved HP genes of P. cryoconitis, designated pcbg5hp1, pcbg5hp2 and pcbg5hp12, were cloned and the proteins were expressed, purified and their functions and structures were evaluated. Real-time quantitative PCR analysis revealed that these genes were expressed constitutively, suggesting a potentially important role where the expression of these genes under an almost constant demand might have some regulatory functions in thermal stress tolerance. Functional analysis showed that these proteins maintained their activities at low and moderate temperatures. Meanwhile, a low citrate synthase aggregation at 43 °C in the presence of PCBG5HP1 suggested the characteristics of chaperone activity. Furthermore, our comparative structural analysis demonstrated that the HPs exhibited cold-adapted traits, most notably increased flexibility in their 3D structures compared to their counterparts. Concurrently, the presence of a disulphide bridge and aromatic clusters was attributed to PCBG5HP1’s unusual protein stability and chaperone activity. Thus, this suggested that the HPs examined in this study acquired strategies to maintain a balance between molecular stability and structural flexibility. Conclusively, this study has established the structure–function relationships of the HPs produced by P. cryoconitis and provided crucial experimental evidence indicating their importance in thermal stress response.