Tolerant Crops Research Articles

Integration of Conventional Breeding and Molecular Tools for Drought Tolerance in Maize: Review

Maize is an important staple crop for food, feed and industry globally. Despite the importance of maize as a principal food crop in developing country, drought is a major constraint that affects maize production, particularly in Sub-Saharan Africa where maize is grown under rainfed condition. Plant breeder has been striving to improve and develop drought tolerance crops. Nevertheless, these efforts still cannot meet the demand of food security due to fast population growth and climatic change. Secondary traits with associated to grain yield, advanced phenotyping, marker assisted selection/QTL, genomic wide association study and genomic selection methods are more expected to help for improvement of drought tolerance in maize. This article discussed the most secondary traits used for selection, plant physiological traits and crossing among drought tolerance elite lines and evaluation their segregants as well as hybrids performance under stress and optimum conditions. Emphasis also given to molecular dissection of drought tolerance complex trats using GWAS and /or gene discovery at hotspot regions for secondary traits, marker-assisted backcrossing and genome selection have been highlighted for increasing genetic gain in grain yield and predicating the breeding value for selection. Thus, further improvement is essential integration of conventional breeding and genomic tools for development of resilience maize varieties for drought tolerance in maize.

Leaf rolling detection in maize under complex environments using an improved deep learning method

Leaf rolling is a common adaptive response that plants have evolved to counteract the detrimental effects of various environmental stresses. Gaining insight into the mechanisms underlying leaf rolling alterations presents researchers with a unique opportunity to enhance stress tolerance in crops exhibiting leaf rolling, such as maize. In order to achieve a more profound understanding of leaf rolling, it is imperative to ascertain the occurrence and extent of this phenotype. While traditional manual leaf rolling detection is slow and laborious, research into high-throughput methods for detecting leaf rolling within our investigation scope remains limited. In this study, we present an approach for detecting leaf rolling in maize using the YOLOv8 model. Our method, LRD-YOLO, integrates two significant improvements: a Convolutional Block Attention Module to augment feature extraction capabilities, and a Deformable ConvNets v2 to enhance adaptability to changes in target shape and scale. Through experiments on a dataset encompassing severe occlusion, variations in leaf scale and shape, and complex background scenarios, our approach achieves an impressive mean average precision of 81.6%, surpassing current state-of-the-art methods. Furthermore, the LRD-YOLO model demands only 8.0 G floating point operations and the parameters of 3.48 M. We have proposed an innovative method for leaf rolling detection in maize, and experimental outcomes showcase the efficacy of LRD-YOLO in precisely detecting leaf rolling in complex scenarios while maintaining real-time inference speed.

Role of iron oxide nanoparticles in maize (Zea mays L.) to enhance salinity stress tolerance

Soils have been getting worse over time, which has led to lower crop yields and nutritional value. This is because of too many conventional fertilizers, anthropogenic activities, and climate change. Soil salinity is also a big problem and challenge for agricultural scientists. To address this issue, nanoparticles are gaining a reputation in agriculture that can enhance salinity tolerance in crops, especially at early growth stages. A pot experiment was conducted at Post Agricultural Research Station (PARS), Faisalabad to assess the impact of iron oxide nanoparticles (0, 15, and 30 ppm) and four levels of salinity (0, 50, 100 and 150 mM) on morphological, physiological and yield traits of maize (Zea mays L.) in salinity stress. Salinity stress significantly negatively affected the growth attributes, photosynthetic pigments, ion content (Na+, K+, and Ca2+) of maize plants. Salinity has also increased the levels of MDA, H2O2, and Na+ ions. The application of iron oxide nanoparticles through foliar spray had a notable impact in enhancing the growth and yield of the tested maize variety. It was achieved by promoting the activities of antioxidant enzymes, increasing photosynthetic pigments, and elevating K+ and Ca2+ ion levels under both normal and salinity-stressed conditions. Additionally, iron oxide nanoparticles mitigated the adverse effects of salinity stress by effectively reducing Na+ ion concentration, MDA levels, and H2O2 concentration. Among the different concentrations tested, 30 ppm of iron oxide nanoparticles proved best in alleviating the negative impacts of salinity stress in maize. Thus, field use of 30 ppm iron oxide nanoparticles as foliar spray could effectively mitigate salinity stress in maize.

Impacts of Climate Change on Streamflow on Dawa Sub-watershed, Genale-Dawa River Basin, Southern Ethiopia

<i>Climate change is statistical variations over an extended period in the features of the climate system, such as variations in global temperatures and precipitation, caused by human and natural sources.</i> In this study aimed to measure and examine how streamflow in the Dawa sub-basin, Genale Dawa River basin was affected by climate change. It used the average of five regional climate models from the Coordinated Regional Climate Downscaling Experiment (CORDEX) Africa, under two different scenarios of Representative Concentration Pathways: RCP4.5 and RCP8.5. The baseline scenario was based on the data from 1975 to 2005, while the future scenarios were based on the data from 2020s (2025–2054) and 2050s (2055–2084). The HBV hydrological model used to assess the impact on streamflow. The HBV model showed good statistical performance in simulating the impact of climate change on streamflow, with a coefficient of determination (R<sup>2</sup>) of 0.88 and Nash-Sutcliffe Efficiency (NSE) of 0.77 for monthly calibration, and R<sup>2</sup> of 0.86 and NSE of 0.83 for monthly validation. The impacts quantified using the mean monthly changes in precipitation, maximum and minimum temperatures. The bias-corrected precipitation and temperature showed a reasonable increase in both future periods for both RCP 4.5 and RCP 8.5 scenarios. These changes in climate variables resulted in a decrease in mean annual streamflow by 1.6 and 3.5% for RCP 4.5 and by 4.6 and 4.9% for RCP 8.5 scenarios of the 2020s and 2050s, respectively. Based on the analysis that predicted a drop in precipitation during the months, and seasons and an increase in precipitation during the <i>Belg</i> season, with a corresponding decrease and rise in stream flow throughout the watershed. So to offset the variation in the watershed, community should adopt various; Soil and water conservation technologies, Using drought tolerant crops, Implementing various trees and appropriate design and applying a water harvesting structure like in-situ, internal or micro catchment, external or macro catchment water harvesting and Surface runoff harvesting. This result offers useful information for current and future water resource management in the basin and similar other watershed in the country.

Rice OsCIPK17-OsCBL2/3 module enhances shoot Na+ exclusion and plant salt tolerance in transgenic Arabidopsis

Soil salinity is detrimental to plant growth and remains a major threat to crop productivity of the world. Plants employ various physiological and molecular mechanisms to maintain growth under salt stress. Identification of genes and genetic loci underlying plant salt tolerance holds the key to breeding salt tolerant crops. CIPK-CBL pathways regulate adaptive responses of plants (especially ion transport) to abiotic stresses via fine-tuned Ca2+ signal transduction. In this study, we showed that over-expression of OsCIPK17 in Arabidopsis enhanced primary root elongation under salt stress, which is in a Ca2+ dependent manner. Further investigation revealed that, under salt stress, OsCIPK17 transcript level was significantly induced and its protein moved from the cytosol to the tonoplast. Using both Y2H and BiFC, tonoplast-localised OsCBL2 and OsCBL3 were shown to interact with OsCIPK17. Interestingly, over-expressing salt-induced OsCBL2 or OsCBL3 in Arabidopsis led to enhanced primary root elongation under salt stress. In this process, OsCIPK17 was shown recruited to the tonoplast (similar to the effect of salt stress). Furthermore, transgenic Arabidopsis lines individually over-expressing OsCIPK17, OsCBL2 and OsCBL3 all demonstrated larger biomass and less Na + accumulation in the shoot under salt stress. All data combined suggest that OsCIPK17- OsCBL2/3 module is a major component of shoot Na+ exclusion and therefore plant salt tolerance, which is through enhanced Na + compartmentation into the vacuole in the root. OsCIPK17 and OsCBL2/3 are therefore potential genetic targets that can be used for delivering salt tolerant rice cultivars.

Metabolism of 2,4-D in plants: comparative analysis of metabolic detoxification pathways in tolerant crops and resistant weeds.

The commercialization of 2,4-D (2,4-dichlorophenoxyacetic acid) latifolicide in 1945 marked the beginning of the selective herbicide market, with this active ingredient playing a pivotal role among commercial herbicides due to the natural tolerance of monocots compared with dicots. Due to its intricate mode of action, involving interactions within endogenous auxin signaling networks, 2,4-D was initially considered a low-risk herbicide to evolve weed resistance. However, the intensification of 2,4-D use has contributed to the emergence of 2,4-D-resistant broadleaf weeds, challenging earlier beliefs. This review explores 2,4-D tolerance in crops and evolved resistance in weeds, emphasizing an in-depth understanding of 2,4-D metabolic detoxification. Nine confirmed 2,4-D-resistant weed species, driven by rapid metabolism, highlight cytochrome P450 monooxygenases in Phase I and glycosyltransferases in Phase II as key enzymes. Resistance to 2,4-D may also involve impaired translocation associated with mutations in auxin/indole-3-acetic acid (Aux/IAA) co-receptor genes. Moreover, temperature variations affect 2,4-D efficacy, with high temperatures increasing herbicide metabolism rates and reducing weed control, while drought stress did not affect 2,4-D efficacy. Research on 2,4-D resistance has primarily focused on non-target-site resistance (NTSR) mechanisms, including 2,4-D metabolic detoxification, with limited exploration of the inheritance and genetic basis underlying these traits. Resistance to 2,4-D in weeds is typically governed by a single gene, either dominant or incompletely dominant, raising questions about gain-of-function or loss-of-function mutations that confer resistance. Future research should unravel the physiological and molecular-genetic basis of 2,4-D NTSR, exploring potential cross-resistance patterns and assessing fitness costs that may affect future evolution of auxin-resistant weeds. © 2024 Society of Chemical Industry.

Multivariate characterization of salicylic acid and potassium induced physio-biochemical and phytoremediation responses in quinoa exposed to lead and cadmium contamination

The levels of soils pollutants such as lead (Pb) and cadmium (Cd) have significantly increased recently resulting in ecological disturbances and threatening crop production. Various amendments have been employed to enhance the tolerance of crops to withstand Cd and Pb stresses. However, the role of combined application of potassium (K) and of salicylic acid (SA) for Cd and Pb stress mitigation and phytoremediation by quinoa (Chenopodium quinoa Willd) has not been comprehended well. In the present study, the effect of 10 mM K and 0.1 mM SA was tested on the quinoa plants subjected to 250 μM Pb and/or 100 μM Cd. The Pb and Cd treatments were applied separately or together. Phytotoxicity induced by Pb and Cd resulted in drastic decrease (>60%) in chlorophyll contents, stomatal conductance, and plant biomass. The collective treatment of Pb and Cd induced an increase in the concentration of hydrogen peroxide (13-fold) and lipid peroxidation (16-fold) that resulted in a 61% reduction in membrane stability. The application of 10 mM K and/or 0.1 mM SA was remarkable in mitigating the adverse effect of Pb and Cd. The reduction in plant biomass was 17% when 10 mM K and 0.1 mM SA were applied together under the combined treatment of both the metals. The simultaneous application of K and SA effectively mitigated oxidative stress by enhancing the activities of superoxide dismutase, peroxidase, ascorbate peroxidase, and catalase by 12, 10, 7 and 10-folds respectively. The positive effect of K and SA on these attributes resulted in a remarkable reduction in metal accumulation and translocation and lipid peroxidation. The stressed plants supplemented with K and SA exhibited a significant improvement in the membrane stability index, chlorophyll content, and stomatal conductance. This study concluded that the combined application of K and SA could be a good approach for reducing Pb and Cd phytotoxicity in quinoa and enhancing their phytostabilization potential in the contaminated soils.

Regev, a New Hybrid Fungicide for Control of Cercospora Leaf Spot on Sugar Beet

Cercospora leaf spot (CLS) is one of the most yield-limiting foliar disease of sugar beet in the United States. Standard practices to control this disease include selection of tolerant varieties, crop rotations, and reliance on fungicides. However, genetic resistance still requires some fungicides for acceptable control under high disease pressure, and there are only a limited number of fungicides registered for control of this disease. Regev® is a newly registered hybrid fungicide for sugar beet disease control that contains eight biological active ingredients from tea tree oil (TTO) and one active ingredient from the synthetic fungicide, difenoconazole. Tea tree oil provides a new and unique mode of activity against both fungal and bacterial plant pathogens, and it has a low resistance risk. Eight trials were conducted to evaluate Regev for efficacy against Cercospora leafspot, and sugar beet yield and quality benefits in Minnesota and Michigan. Five of the trials had slightly different treatments with the primary goal of allowing trialists to conduct trials with treatments that were appropriate for their locations. However, one of the trials at the Northwest Research and Outreach Center (NWROC) of the University of Minnesota in Crookston, Minnesota, was conducted three times over the years 2021, 2022, and 2023. Across the eight trials, spray programs that included Regev provided control of Cercospora leaf spot equal to standard fungicide programs.

(Invited) Carbon Nanodots for Improved Plant Genetic Engineering

Anthropogenic climate change has made the need for more nutritious and stress-tolerant plants indisputable for providing food security. Climate change has decreased the global agricultural productivity by 21% since 1961, and will continue to drop crop yields by 7.4% for every degree-Celsius increase in temperature. CRISPR genetic engineering can enable previously inaccessible crop tolerances to meet the demand for nutritionally-balanced and high-yielding crops. However, plant biomolecule delivery and transformation are two important bottlenecks that prevent plant genetic engineering from reaching its full potential.Nanomaterials have already shown promise to deliver genetic cargoes into plant somatic cells for transient expression of proteins1. For most applications in agriculture, however, biomolecules need to be delivered to cells that can give rise to next generation of edited plants, such as germ or stem cells. These cell types are extremely challenging to penetrate and deliver cargoes using traditional approaches, hence forcing researchers to use undifferentiated tissues to transform and go through an arduous regeneration process in tissue culture. This time consuming, inefficient, and highly species dependent approach has been a critical obstacle in plant biology and bioengineering.We have synthesized small (~3 nm) and positively charged (+35 mV) carbon nanodots (CNDs) from glycerol and polyethyleneimine (PEI) using the microwave method. These CNDs showed high entry efficiency into plant stem cells and ability to deliver DNA cargoes into a model plant Nicotiana benthamiana without affecting stem cell viability and differentiation capability. This is a first-time demonstration of nanoparticle uptake by plant stem cells and cargo expression in these cell types. Once we perform CRISPR gene editing in these stem cells, the edited germ cells and seeds will develop automatically from the stem cells, facilitating the creation of edited and improved new plant varieties.

Design, Synthesis and Proteomics-Based Analysis of Novel Triazinone Derivatives Containing Amide Structures as Safer Protoporphyrinogen IX Oxidase Inhibitors.

Resistant weeds severely threaten crop yields as they compete with crops for resources required for survival. Trifludimoxazin, a protoporphyrinogen IX oxidase (PPO) inhibitor, can effectively control resistant weeds. However, its crop safety record is unsatisfactory. Consequently, a scaffold-hopping strategy is employed in this study to develop a series of new triazinone derivatives featuring an amide structure. Most compounds depicted excellent herbicidal activity across a broad spectrum at 37.5-150 g ai/ha, among which (R)-I-5 was equivalent to flumioxazin. (R)-I-5 demonstrated significant crop tolerance to rice and wheat, even at 150 g ai/ha. (R)-I-5 exhibited superior pharmacokinetic features compared to flumioxazin and trifludimoxazin. This was depicted by the absorption, distribution, metabolism, excretion, and toxicity predictions. Notably, proteomics-based analysis was applied for the first time to investigate variations among plant proteins before and after herbicide application, shedding light on the conservative and divergent roles of PPO.

Microalgae- and cyanobacteria-derived phytostimulants for mitigation of salt stress and improved agriculture

Soil salinization poses severe abiotic stress that adversely affects plant growth and development, ultimately threatening global food security by inducing physiological abnormalities. In response to escalating nutrient demands, with global requirements quantified at 76 % for nitrogen and 87 % for phosphorus, modern agriculture is increasingly adopting sustainable practices to enhance nutrient recycling and reduce reliance on external inputs. Emerging sources of plant phytostimulants, such as microalgal and cyanobacterial biomass, show promise in augmenting crop yields and bolstering plant resistance to various abiotic factors, including salt stress. The efficacy of these microorganisms stems from their simplistic cellular structure, superior photosynthetic efficiency, capacity for heterotrophic growth, adaptability to varying environmental conditions, potential for metabolic engineering, and the abundance of valuable biomolecules (such as soluble amino acids, micronutrients, polysaccharides, and phytohormones) within their biomass. This review provides an analysis of the current research landscape concerning microalgae- and cyanobacteria-derived phytostimulants, highlighting their promise as an innovative and sustainable alternative to synthetic fertilizers in the agricultural sector. Moreover, it identifies various adaptive responses of plants to salinity stress and assesses the potential and challenges associated with the use of microalgae and cyanobacteria-based metabolites for developing new sustainable strategies to enhance crop tolerance to salinity stress.

Crescimento e homeostase iônica de mudas de pinha irrigadas com águas residuais salinas

ABSTRACT Custard apple (Annona squamosa L.) is a sensitive fruit crop to salinity. One of the alternatives to minimize the deleterious effects of salt stress is adopting mineral fertilization management practices, promoting increased crop tolerance to salts. Therefore, the objective of the present study was to evaluate NPK doses to mitigate salt stress in custard apple seedlings irrigated with saline wastewater. The experiment was performed in a greenhouse, adopting a randomized block design in a 3 × 5 factorial scheme, corresponding to three irrigation waters (local-supply water of 0.53 dS m-1 electrical conductivity (control); desalination reject of 3.5 dS m-1; fish farming effluent of 3.5 dS m-1) and five proportions of the NPK recommendation (25, 50, 75, 100, and 125% of the fertilization recommendation), with four replicates. The growth, biomass accumulation, and nutrients were evaluated. Irrigation with desalination reject, and fish farming effluent restricted the growth of custard apple seedlings, regardless of the NPK dose used. The ionic homeostasis of seedlings irrigated with desalination reject and fish farming effluent is optimized by fertilization with 50% of the NPK recommendation corresponding to 50, 150, and 75 mg of N, P2O5, and K2O dm-3, respectively. For the production of seedlings irrigated with local-supply water, the dose of 75% NPK, corresponding to 75, 225, and 112.5 mg of N, P2O5, and K2O dm-3, respectively, is recommended.

Individual or successiveseed priming with nitric oxide and calcium toward enhancing salt tolerance of wheat crop through early ROS detoxification and activation of antioxidant defense

Despite the considerable efforts reported so far to enhance seed priming, novel ideas are still needed to be suggested to this sustainable sector of agri-seed industry. This could be the first study addressing the effect of nitric oxide (NO) under open field conditions. The impacts of seed redox-priming using sodium nitroprusside (SNP) and osmo-priming with calcium chloride (CaCl2), both applied individually or successively, were investigated under salinity stress conditions on wheat plants (Triticum aestivum L.). Various parameters, including water relations, growth, yield, photosynthetic pigments, and antioxidant activities (enzymatic and non-enzymatic), were recorded to assess the outcomes of these priming agents on mitigating the negative impacts of salinity stress on wheat plants. Water consumptive use (ETa) and irrigation water applied (IWA) decreased with seeds priming. Successive priming with SNP + CaCl2 induced the greatest values of crop water productivity (CWP), irrigation water productivity (IWP), seed index, grain yield and grain nitrogen content.Under salinity stress, the dry weight of plants was decreased. However, hydro-priming and successive chemical priming agents using combinations of calcium chloride and sodium nitroprusside (CaCl2 + SNP & SNP + CaCl2) preserved growth under salinity stress.Individual priming with sodium nitroprusside (SNP) and calcium chloride (CaCl2) resulted in the lowest recorded content of sodium in the shoot, with a value of 2 ppm. On the other hand, successive priming using CaCl2 + SNP or SNP + CaCl2 induced the contents of potassium in the shoot, with values of 40 ppm and 39 ppm, respectively. Malondialdehyde decreased in shoot significantly withapplicationof priming agents. Successive priming with CaCl2 + SNP induced the highest proline contents in shoot (6 µg/ g FW). The highest value of phenolics and total antioxidants contents in shoot were recorded under successive priming using CaCl2 + SNP and SNP + CaCl2.Priming agents improved the activities of ascorbate peroxidase and catalase enzymes. The successive priming improved water relations (ETa, IWA, CWP and IWP) and wheat growth and productivity under salinity stress more than individual priming treatments.

CRISPR-Cas9 mediated understanding of plants' abiotic stress-responsive genes to combat changing climatic patterns.

Multiple abiotic stresses like extreme temperatures, water shortage, flooding, salinity, and exposure to heavy metals are confronted by crop plants with changing climatic patterns. Prolonged exposure to these adverse environmental conditions leads to stunted plant growth and development with significant yield loss in crops. CRISPR-Cas9 genome editing tool is being frequently employed to understand abiotic stress-responsive genes. Noteworthy improvements in CRISPR-Cas technology have been made over the years, including upgradation of Cas proteins fidelity and efficiency, optimization of transformation protocols for different crop species, base and prime editing, multiplex gene-targeting, transgene-free editing, and graft-based heritable CRISPR-Cas9 approaches. These developments helped to improve the knowledge of abiotic stress tolerance in crops that could potentially be utilized to develop knock-out varieties and over-expressed lines to tackle the adverse effects of altered climatic patterns. This review summarizes the mechanistic understanding of heat, drought, salinity, and metal stress-responsive genes characterized so far using CRISPR-Cas9 and provides data on potential candidate genes that can be exploited by modern-day biotechnological tools to develop transgene-free genome-edited crops with better climate adaptability. Furthermore, the importance of early-maturing crop varieties to withstand abiotic stresses is also discussed in this review.

Assessment of waterlogging-induced changes in enzymatic antioxidants and carbohydrate metabolism in peanuts genotypes

Soil flooding, manifesting as submergence or waterlogging stress, significantly impacts plant species composition and agricultural productivity, particularly in regions with low rainfall. This study investigates the biochemical responses of two peanut (Arachis hypogaea L.) genotypes, DH-86 and GJG-32, under waterlogging stress. The experiment involved in-vivo pot trials where peanut plants were subjected to continuous waterlogging for 12 days at the flowering stage. Biochemical analyses of leaves conducted and revealed significant alterations in enzyme activities and metabolite concentrations. Key findings include variations in superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPOD), α-amylase, invertase, acid phosphomonoesterase activities, and changes in starch, proline, reducing sugars, and chlorophyll content. SOD, CAT, and GPOD activities exhibited differential responses between genotypes, highlighting DH-86's quicker recovery post-waterlogging. Notably, DH-86 demonstrated higher resilience, reflected in its rapid normalization of biochemical parameters, while GJG-32 showed prolonged stress effects. These findings underscore the importance of antioxidative enzyme systems in mitigating oxidative damage induced by waterlogging. This study enhances our understanding of the biochemical adaptations of peanut genotypes to waterlogging stress, offering valuable insights for breeding programs focused on improving flood tolerance in crops.

Stress Management Practices in Rainfed Sorghum (K12) on Yield, Economics and Plant Nutrient Uptake Through Foliar Plant Nutrition Under Typical Vertisols (Typic chromusterts) of Semiarid Region

ABSTRACT Foliar fertilization has great potential to give higher yields under intensive cropping system and in enhancing the crop tolerance to drought conditions. Different responses of yield to foliar fertilization are probably associated with incorrect timing of application, use of inappropriate fertilizer and lack of concern of soil available nutrients and environmental conditions. Though soil application of nutrients cannot be replaced totally with foliar fertilization, but under various situations like rainfed condition, foliar nutrition can play very important role in enhancing crop yield and nutrient use efficiency. Focusing on this, a field experiment was conducted in the Agricultural Research Station, Kovilpatti, under rainfed situation from 2017 to ‘20 to evaluate the response of foliar application of plant nutrients sprayed during moisture stress condition and to improve the growth and yield of sorghum under rainfed condition. This experiment was laid out in split plot design with replicated thrice and the treatments consisted of main plots: M1 – foliar spray during dry spell, M2 – foliar spray after relieving of stress/dry spell (with favorable soil moisture) and subplots: S1 – urea@1%, S2 – urea@2%, S3 – water soluble complex fertilizer (19:19:19)@0.5%, S4 – water soluble complex fertilizer (19:19:19)@0.5% + recommended dose of micronutrient for foliar spray (to be decided based on soil test: zinc, boron, iron, manganese, etc.), S5 – ZnSO4@0.5%, S6 – water spray, S7 – control (no spray of any material/water) under rainfed vertisols of semiarid region. The obtained results showed that the application of water soluble complex fertilizer (19:19:19)@0.5% sprayed during the dry spell significantly increased the plant height, earhead length and grain test weight. This attributes resulted in higher grain yield (1448 kg ha−1), stover yield (2288 kg ha−1), net returns (Rs. 8748 ha−1), B:C (1.16) and rainwater use efficiency (3.85 kg ha−1-mm) when compared to other practices in rainfed sorghum. There is a gradual increase in total major plant nutrient uptake, viz. nitrogen (22.3 kg ha−1), phosphorus (4.56 kg ha−1) and potassium uptake (26.0 kg ha−1) with the foliar application of water soluble complex fertilizer (19:19:19)@0.5% applied during the dry spell condition (S3) during drought stress condition (M1). Foliar application of nutrients along with soil application has several advantages in supplementing the nutritional requirements of crops such as rapid and efficient response by the plants, less product needed and independence of soil conditions. Foliar nutrition designed to eliminate the problems like fixation and immobilization of nutrients. Hence, foliar nutrition is recognized as an important method of fertilization during the dry spell under rainfed condition in modern agriculture.

Unlocking Rapid and User-Friendly Strategies to Improve Horticultural Crop Qualities

Climatic changes and global warming affect the growth, development, and productivity of crops. In this review, we highlight the possible benefits of using innovative breeding techniques like clustered regularly interspaced short palindromic repeats (CRISPRs), exogenous phytohormone-like strigolactones (SLs), nanomaterials (NMs), and beneficial microbial endophytes to address the challenges in sustainable cultivation of horticultural crops. These applications are evaluated by examining how they affect different metabolic, morphological, and biochemical parameters in diverse crops. Endophytes are symbiotic microorganisms and can be used as nematicides for improving crop yield. With an emphasis on quality control, we examined the impacts of applying NMs, a novel family of phytohormones called SLs, and microbial endophytes on horticultural commodities. Furthermore, we reviewed the benefits of CRISPR for the editing of plant genomes, as well as how it affects gene expression and transcription factors to increase crop tolerance and yield. These innovations hold the potential to improve crop yield, quality, and resilience by acting as safe, natural components in biofertilizers and plant protection solutions. Gradually adopting these methods could decrease reliance on agrochemicals, thereby reducing their negative effects on biodiversity, soil fertility, and human health.

Dynamic interplay of reactive oxygen and nitrogen species (ROS and RNS) in plant resilience: unveiling the signaling pathways and metabolic responses to biotic and abiotic stresses.

Plants respond to environmental challenges by producing reactive species such as ROS and RNS, which play critical roles in signaling pathways that lead to adaptation and survival strategies. Understanding these pathways, as well as their detection methods and effects on plant development and metabolism, provides insight into increasing crop tolerance to combined stresses. Plants encounter various environmental stresses (abiotic and biotic) that affect plant growth and development. Plants sense biotic and abiotic stresses by producing different molecules, including reactive species, that act as signaling molecules and stimulate secondary messengers and subsequent gene transcription. Reactive oxygen and nitrogen species (ROS and RNS) are produced in both physiological and pathological conditions in the plasma membranes, chloroplasts, mitochondria, and endoplasmic reticulum. Various techniques, including spectroscopy, chromatography, and fluorescence methods, are used to detect highly reactive, short-half-life ROS and RNS either directly or indirectly. In this review, we highlight the roles of ROS and RNS in seed germination, root development, senescence, mineral nutrition, and post-harvest control. In addition, we provide information on the specialized metabolism involved in plant growth and development. Secondary metabolites, including alkaloids, flavonoids, and terpenoids, are produced in low concentrations in plants for signaling and metabolism. Strategies for improving crop performance under combined drought and pathogen stress conditions are discussed in this review.

Regulation of nitro-oxidative homeostasis: an effective approach to enhance salinity tolerance in plants.

Soil salinity is a major constraint for sustainable agricultural productivity, which together with the incessant climate change may be transformed into a severe threat to the global food security. It is, therefore, a serious concern that needs to be addressed expeditiously. The overproduction and accumulation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) are the key events occurring during salt stress, consequently employing nitro-oxidative stress and programmed cell death in plants. However, very sporadic studies have been performed concerning different aspects of nitro-oxidative stress in plants under salinity stress. The ability of plants to tolerate salinity is associated with their ability to maintain the cellular redox equilibrium mediated by both non-enzymatic and enzymatic antioxidant defense mechanisms. The present review emphasizes the mechanisms of ROS and RNS generation in plants, providing a detailed evaluation of how redox homeostasis is conserved through their effective removal. The uniqueness of this article stems from its incorporation of expression analyses of candidate genes for different antioxidant enzymes involved in ROS and RNS detoxification across various developmental stages and tissues of rice, utilizing publicly available microarray data. It underscores the utilization of modern biotechnological methods to improve salinity tolerance in crops, employing different antioxidants as markers. The review also explores how various transcription factors contribute to plants' ability to tolerate salinity by either activating or repressing the expression of stress-responsive genes. In summary, the review offers a thorough insight into the nitro-oxidative homeostasis strategy for extenuating salinity stress in plants.

Growth, yield, and fiber quality of cotton plants under drought stress are positively affected by seed priming with potassium nitrate

Growth, productivity, and fiber quality of cotton (Gossypium hirsutum L.), a vital fiber-producing cash crop, are severely affected under drought conditions. Seed priming has a proven role in enhancing crop tolerance to abiotic stress, including drought. The objective of this study was to evaluate the effects of seed priming with KNO3 on cotton productivity and fiber quality under drought stress. A germination experiment was established under laboratory conditions with five treatments of seed priming (non-primed or control treatment, hydropriming, and priming with 2.5, 5.0, and 7.5 g KNO3 L−1). Another experiment under polyhouse conditions based on the same seed priming treatments was conducted under three levels of soil water contents (field capacity [FC] 100%: FC100, 75%: FC75, and 50%: FC50). The results obtained showed that there was a clear reduction in the different parameters tested at FC50 in comparison to FC100 (39–54%, 32–44%, 6–12%, and 7–12% reduction for boll number per plant, seed cotton yield, fiber strength, and leaf relative water content, respectively, across all priming treatments). Seed priming with KNO3 at 5 g L−1 effectively alleviated the detrimental effects originated from drought stress and caused 61–73%, 13–16%, and 16–23% increase in seed cotton yield, fiber length, and fiber strength, respectively, across soil moisture levels when compared with the control treatment. The same KNO3 dose caused an increase of 78% in water productivity in comparison to the control plants at FC50. Priming cotton seeds with 5 g KNO3 L−1 holds promise to obtain synchronized germination, enhance fiber quality, and increase yield, especially under water-limited conditions, thereby promoting economic viability and environmental sustainability. Additionally, this practice enhances water productivity, leading to significant water savings and reduced irrigation costs for cotton farmers. This method could be used as potential technology in advancing sustainable agriculture in water-constraint conditions.