Tolerance Range Research Articles

Engineering Synechococcus sp. PCC 7002 for sustainable production of sucrose

Sucrose is a readily available substrate and feedstock for biotechnology, which is biosynthesized by plants, algae, and cyanobacteria. Engineered cyanobacteria with enhanced sucrose production may be a viable alternative to sucrose synthesis from plants. The marine cyanobacteria which can be cultivated in seawater have the potential to provide a sustainable solution. The model marine cyanobacterium Synechococcus sp. PCC 7002 (Syn7002) has many features for a good chassis, including a fast growth rate and a wide range of stress tolerances. Syn7002 synthesizes mainly glucosylglycerol and traces of sucrose as compatible solutes under salt stress. The compatible solute glucosylglycerate was also found in small amounts in Syn7002. To enhance sucrose production in Syn7002, the sucrose biosynthesis genes and the sucrose transporter gene were overexpressed. And the competing pathway genes and the sucrose hydrolysis gene were inactivated. The sucrose production in the engineered strain can reach 2282 mg/L in 8 days under 1 M NaCl stress. With the further substitution of sucrose biosynthesis genes from Synechocystis sp. PCC 6803, the engineered strain can efficiently produce sucrose over a wide range of NaCl concentrations. The efficient sucrose production by marine cyanobacteria will promote the sustainable supply of sugar feedstocks for biotechnology and aquaculture.

Content of Sodium, Potassium, Calcium, Magnesium in Organism of Larva of Sibling Species <i>Сhironomus balatonicus</i> and <i>Сhironomus plumosus</i> in Dependence on Salinity<sup>1</sup>

The tolerant salinity ranges are 0.008–6 and 0.09–9.3 g/L, respectively, for Ch. plumosus L., 1758 and Ch. balatonicus Devai, Wülker et Scholl, 1983. In the tolerant range of salinity, the level of sodium, potassium, calcium, magnesium in the body of Ch. balatonicus was regulated within 33.9–77.1, 7.7–13.7, 11.9–37.6, 54.7–110.6 mmol/kg wet weight, respectively. The concentration of sodium, potassium, calcium, magnesium in the body of Ch. plumosus was maintained within of 62.7–80.8, 10.6–18, 6.7–12, 2.9–6 mmol/kg wet weight in stage III larvae and 49.6–62.3, 9.7–14.8, 42.5–62.2, 47.9–83.4 mmol/kg wet weight in individuals of IV age. In the optimal salinity range of 0.8–5.3 g/L, the sodium concentration in the body of Ch. balato-nicus was maintained at a constant level, decreased in the critically low zone of 0.8–0.09 g/L, and increased in the critically high zone of 5.3–9.3 g/L. In the critically low and high salinity zone, the organism’s ability to survive is reduced. The content of sodium in the body of freshwater aquatic organisms serves as a reliable criterion for assessing the optimal, critically low and high salinity zones.

A Short Review of Asian Seabass (Lates calcarifer) Cultivation

Aquaculture is the most reliable sector to the providing world nutrias food. It is all depended on the cultivable species. Asian seabass is one of the candidate species for aquaculture because of the wide range of water quality tolerance, growth performance, and consumer preference. The Indian and Western Pacific Oceans are where Asian seabass is found in its natural habitat. It can be cultured in the earthen pond, floating or stationer cages, and recirculating system. Seed resources are available in rivers and lakes of fresh water, but aquaculture is the depending on the hatchery that produces seed because of the superior growth production. In the nursery phase, cannibalism is predominant in Asian seabass cultivation, which will improve via the grading of a shooter. Feeding is one of the important management for good growth performance and reducing cannibalism to give the optimum feed requirements. The growth rate of the Asian seabass is 400-600 g in 4 to 6 months. Asian seabass farmers are suffering from some infectious and non-infection diseases, it will improve via the good management practices of the culture promises. In this review paper, some key points of Asian seabass farming are covered for better understanding.

Cobalt-Catalyzed Hydroxymethylation of Alkyl Halides with CO as the C1 Source.

Herein, we developed an effective strategy for the synthesis of one-carbon-extended alcohols through cobalt-catalyzed hydroxymethylation of alkyl halides with carbon monoxide as the C1 source and cheap and environmentally friendly PMHS as the hydride source. This procedure also features a ligand-free cobalt catalyst and a broad range of functional group tolerance.

Facilitating structural elucidation of small environmental solutes in RPLC-HRMS by retention index prediction

Implementing effective environmental management strategies requires a comprehensive understanding of the chemical composition of environmental pollutants, particularly in complex mixtures. Utilizing innovative analytical techniques, such as high-resolution mass spectrometry and predictive retention index models, can provide valuable insights into the molecular structures of environmental contaminants. Liquid Chromatography-High-Resolution Mass Spectrometry is a powerful tool for the identification of isomeric structures in complex samples. However, there are some limitations that can prevent accurate isomeric structure identification, particularly in cases where the isomers have similar mass and fragmentation patterns. Liquid chromatographic retention, determined by the size, shape, and polarity of the analyte and its interactions with the stationary phase, contains valuable 3D structural information that is vastly underutilized. Therefore, a predictive retention index model is developed which is transferrable to LC-HRMS systems and can assist in the structural elucidation of unknowns. The approach is currently restricted to carbon, hydrogen, and oxygen-based molecules <500 g mol−1. The methodology facilitates the acceptance of accurate structural formulas and the exclusion of erroneous hypothetical structural representations by leveraging retention time estimations, thereby providing a permissible tolerance range for a given elemental composition and experimental retention time. This approach serves as a proof of concept for the development of a Quantitative Structure-Retention Relationship model using a generic gradient LC approach. The use of a widely used reversed-phase (U)HPLC column and a relatively large set of training (101) and test compounds (14) demonstrates the feasibility and potential applicability of this approach for predicting the retention behaviour of compounds in complex mixtures. By providing a standard operating procedure, this approach can be easily replicated and applied to various analytical challenges, further supporting its potential for broader implementation.

Photoinduced Arene C−H Amination with Ammonia: A Practical and Regioselective Strategy for Primary Amines

AbstractIntroducing the primary amino group into aromatic ring is one of the most important study issues in organic chemistry, particularly for the pharmaceuticals and agrochemicals chemistry. Herein, we describe a photoinduced direct C−H amination of arenes with ammonia via the site‐selective C−H thianthrenation that forms a new C−N bond with excellent regioselectivity. The reaction is carried out under mild conditions, and with a wide range of functional group tolerance, such as sensitive −Cl, −Br, and −OH groups which are poorly tolerated in conventional approaches. Moreover, the synthetic utility of our amination protocol has been confirmed through late‐stage modification and gram‐scale synthesis of complex drug‐like molecules.

Trajectory planning method for docking of large aircraft components

PurposeIn the digital assembly system of large aircraft components (LAC), the docking trajectory of LAC is an important factor affecting the docking accuracy and stability of the LAC. The main content of docking trajectory planning is how to move the LAC from the initial posture and position to the target posture and position (TPP). This paper aims to propose a trajectory planning method of LAC based on measured data.Design/methodology/approachFirst, the posture and position error model of the wing is constructed according to the measured data of the measurement points (MPs) and the fork lug joints. Second, the particle swarm optimization algorithm based on the dynamic inertia factor is used to optimize the TPP of the wing. Third, to ensure the efficiency and stability of posture adjustment, the S-shaped curve is used as the motion trajectory of LAC, and the parameters of the trajectory are solved by the generalized multiplier method. Finally, a series of docking experiments are carried out.FindingsDuring the process of posture adjustment, the motion of the numerical control locator (NCL) is stable, and the interaction force between the NCLs is always within a reasonable range. After the docking, the MPs are all within the tolerance range, and the coaxiality error of the fork lug hole is less than 0.2 mm.Originality/valueIn this paper, the measured data rather than the theoretical design model is used to solve the TPP, which improves the docking accuracy of LAC. Experiment results show that the proposed trajectory method can complete the LAC docking effectively and improve the docking accuracy.

Development of Thermally Stable Nanobodies for Detection and Neutralization of Staphylococcal Enterotoxin B.

In this study, sixteen unique staphylococcal enterotoxin B (SEB)-reactive nanobodies (nbs), including ten monovalent and six bivalent nbs, were developed. All characterized nbs were highly specific for SEB and did not cross-react with other staphylococcal enterotoxins (SE). Several formats of highly sensitive enzyme-linked immunosorbent assays (ELISAs) were established using SEB nbs and a polyclonal antibody (pAb). The lowest limit of detection (LOD) reached 50 pg/mL in PBS. When applied to an ELISA to detect SEB-spiked milk (a commonly contaminated foodstuff), a LOD as low as 190 pg/mL was obtained. The sensitivity of ELISA was found to increase concurrently with the valency of nbs used in the assay. In addition, a wide range of thermal tolerance was observed among the sixteen nbs, with a subset of nbs, SEB-5, SEB-9, and SEB-62, retaining activity even after exposure to 95 °C for 10 min, whereas the conventional monoclonal and polyclonal antibodies exhibited heat-labile properties. Several nbs demonstrated a long shelf-life, with one nb (SEB-9) retaining 93% of its activity after two weeks of storage at room temperature. In addition to their usage in toxin detection, eleven out of fifteen nbs were capable of neutralizing SEB's super-antigenic activity, demonstrated by their inhibition on IL-2 expression in an ex vivo human PBMC assay. Compared to monoclonal and polyclonal antibodies, the nbs are relatively small, thermally stable, and easy to produce, making them useful in applications for sensitive, specific, and cost-effective detection and management of SEB contamination in food products.

Ratiometric detection of Zn(II) and Cd(II) ions by a benzobisimidazole based probe in aqueous systems and theoretical calculations

The probe 2,6-di(2-pyridyl)-1,5-dihydroimidazo[4,5-f]benzimidazole (LH2) was investigated for its sensing application towards metal ions. This probe can detect Zn2+ and Cd2+ ions in three different aqueous systems viz., water, DMSO/HEPES buffer (1:1, pH = 7.34, rt), and DMSO/water (1:1, rt). In water a “turn-on” response was observed for both metal ions, whereas in the later two solvent systems, a ratiometric change in fluorescence maximum was observed. The detection limit of this probe is as low as 0.3 μM and 0.62 μM (in water) for Zn2+ and Cd2+ ions, respectively. In both DMSO/HEPES buffer and DMSO/water (1:1), LOD values were identically 1.67 μM (for Zn2+) and 1.93 μM (for Cd2+). This probe has a good pH tolerance range which is 4–8 (for Zn2+) and 4–11 (for Cd2+). Lehrer and Chipman plot and Job’s plot suggested a 2:1 binding ratio between metal ion and LH2. The high binding constants obtained from the Lehrer and Chipman method are suggestive of the formation of a very stable complex in the solution. The binding of Zn2+ and Cd2+ ions to the probe was further confirmed by the NMR titration method which indicated the intensity of N–H protons is halved upon addition of the respective metal ion solution in DMSO‑d6. Reversibility of the detection was also established using Na2EDTA solution. DFT/TD-DFT calculation supported the experimentally observed red-shift of the absorption band.

Modeling and optimizing concentration of exogenous application of γ-aminobutyric acid on NaCl-stressed pineapple mint (Mentha suaveolens) using response surface methodology: an investigation into secondary metabolites and physiological parameters

Salinity, a severe worldwide issue, compromises the economic production of medicinal plants including mints and causes drug-yield decline. γ-Aminobutyric acid (GABA) is a tolerance-inducing signaling bio-molecule in various plant physiological processes. Pineapple mint (Mentha suaveolens Ehrh.) is a valuable medicinal herb with an exhilarating scent of citrus fruit. Piperitenone oxide is the major bioactive constituent of its essential oil, having significant demand by pharmaceutical industries. Nonetheless, modeling and optimizing the effective concentration of GABA remain within twin foci of interest. Therefore, a two factor-five level (NaCl 0-150 mM and GABA 0-2.4 mM) central composite design was conducted to model and optimize drug yield and physiological responses of M. suaveolens. Based on the design of experiments (DoE) approach, different linear, quadratic, cubic, and quartic models were assigned to the response variables. Change trends of shoot and root dry weights followed a simple linear model, whereas sophisticated models (i.e., multiple polynomial regression) were fitted to the other traits. NaCl stress inevitably reduced root and shoot dry weight, piperitenone oxide content, relative water content, pigments content, and maximum quantum yield of PSII. However, content of malondialdehyde (MDA) and total flavonoid, and DPPH radical scavenging activity were increased under salinity. Under severe NaCl stress (150 mM), the essential oil content (0.53%) was increased three times in comparison with control (0.18%). Optimization analysis demonstrated that the highest amount of essential oil (0.6%) and piperitenone oxide (81%) as a drug yield-determining component would be achievable by application of 0.1–0.2 mM GABA under 100 mM NaCl. The highest dry weight of root and shoot was predicted to be achieved at 2.4 mM GABA. Overall, extremely severe NaCl stress (i.e., more than 100 mM) in which a sharp drop in yield components value was observed seemed to be out of M. suaveolens salinity tolerance range. Hence, it is rationale to compensate the decrease of drug yield by foliar application of a dilute GABA solution (i.e., 0.1–0.2 mM) under 100 mM NaCl stress or lower levels.

Warmer and more acidic conditions enhance performance of an endemic low shore gastropod.

Changing ocean temperatures are predicted to challenge marine organisms, especially when combined with other factors, such as ocean acidification. Acclimation, as a form of phenotypic plasticity, can however, moderate the consequences of changing environments for biota. Our understanding of how altered temperature and acidification together influence species acclimation responses is, however, limited compared to responses to single stressors. This study investigated how temperature and acidification affected the thermal tolerance and righting speed of the Girdled Dogwhelk, Trochia cingulata (Linnaeus, 1771). Whelks were acclimated for two weeks to combinations of three temperatures (11°C: cold, 13°C: moderate and 15°C: warm) and two pH regimes (8.0: moderate and 7.5: acidic). We measured the temperature sensitivity of righting response by generating thermal performance curves from individual data collected at seven test temperatures and determined critical thermal minima (CTmin) and maxima (CTmax). We found that T. cingulata has a broad basal thermal tolerance range (∼38°C) and after acclimation to the warm temperature regime, both the optimal temperature for maximum righting speed and CTmax increased. Contrary to predictions, acidification did not narrow this population's thermal tolerance but increased CTmax. These plastic responses are likely driven by the predictable exposure to temperature extremes measured in the field which originate from the local tidal cycle and the periodic acidification associated with ocean upwelling in the region. This acclimation ability suggests that T. cingulata has at least some capacity to buffer the thermal changes and increased acidification predicted to occur with climate change.

Copepod community structure in the highly turbid innermost part of Ariake Bay in the 2000s with special reference to high abundances of &lt;i&gt;Oithona davisae&lt;/i&gt;

Ariake Bay has Japan’s biggest nori seaweed farming field in the innermost part and has suffered from environmental degradation since the late 1990s. To elucidate the seasonal patterns in the copepod community structure in the inner bay, we examined monthly plankton-net samples collected in the top 2-m layer at 11 stations for 5 years from April 2002 to March 2007. Of the 11 stations, 7 were at the high-turbidity site (HTS) representing the nori farming field and 4 in the offshore low-turbidity site (LTS) for comparison. The copepod community in the HTS was dominated almost year round by Oithona davisae, whose 5-year mean abundance of 203 ind. L−1 comprised 84% of copepods and was more than double that in the LTS. O. davisae became extremely abundant in summer with the maximum monthly mean abundance in the HTS of 2756 ind. L−1 and the maximum sample-specific abundance of 10116 ind. L−1. The following predominant copepods in the HTS were Parvocalanus crassirostris in summer–autumn, Microsetella norvegica in summer, Acartia omorii in spring, and Paracalanus parvus s.l. irregularly. Possible causes for high abundance of O. davisae in the HTS are assumed to be sufficient food resources, water temperature over 21°C, and a wide salinity tolerance range of the species.

Bamboo Spreading Impacts as a Watershed Conservation Strategy in Upstream Area of Three Big Rivers of Sumatra

Water is the source of life. The uncontrolled use of natural resources is currently causing the massive pollution of rivers in Sumatra, so the availability of clean water for people's lives is started to decrease. One of the efforts to conserve watersheds is to preserve riparian vegetation. Where there is a source of water, there is bamboo. Bamboo that grows in riparian areas has a root system that can prevent erosion and filter water, increasing clean water availability. The purpose of this study was to conduct an inventory, study of ecology, and indicate environmental factors of bamboo species that can potentially maintain riparian environmental conditions, which can later be developed as soil and water conservation plants in the Sumatran watershed. This research was conducted in the riparian areas in upstream of three major Sumatran rivers: Kuantan, Kampar, and Rokan. We recorded 20 species of bamboo plants from 5 genera in three major rivers of Sumatra. The Rokan watershed has environmental conditions that are still maintained with low human activity so that the diversity of bamboo upstream of the Rokan river is high and is still well maintained. Meanwhile, the Kuantan and Kampar watersheds have a lot of human activities along the river, so the bamboo vegetation has been exploited in these two areas. Based on Canonical Correspondence Analysis, Bambusa vulgaris is found suitable for cultivation in Kuantan and Kampar watersheds, which is abundant in these watersheds as well as has high tolerance range for polluted watershed. We recommend that B. vulgaris be used as riparian vegetation for soil and water conservation in polluted watersheds.

Distributed focusing reduces mirror error sensitivity on x-ray beamlines.

The appearance of very low emittance, high-power synchrotron sources has resulted in ever longer beamlines, often requiring a very weak curvature on the mirrors that transport the beam to the experiment, where the radius of curvature is on the order of kilometers. Manufacturing weakly curved, low figure error grazing incidence mirrors is difficult as the mirrors must be manufactured to an accuracy comparable to the wavelength of the transmitted light. Often the delivered mirrors have figure errors at various length scales (general shape, slope errors, roughness), which compromise image quality. An error in general shape, like the radii of a toroidal mirror, results in long-sighted or short-sighted imaging that is not so simply corrected by changing the distances and incidence angles as the mirror controls the beam focus in both vertical and horizontal directions; for a toroidal mirror, the tangential and sagittal radii need to match correctly for the desired focusing effect. Adaptive downstream optics can compensate for this. In this paper, an alternative method to reduce the sensitivity to a large radius error outside the specified tolerance range in the first mirror of a plane grating monochromator beamline at MAX IV is presented. It is found that distributed focusing by two passive, fixed radius mirrors reduces greatly the sensitivity to the radius errors in both mirrors. The radius tolerance of a mirror initially found to be unacceptable for single stage focusing is easily accommodated on both mirrors in distributed focusing, without compromising the imaging capability.

Effects of primary aberrations on a dark-field microscopy imaging system with the sharper focusing lens

The effects of primary aberrations of the sharper focusing lens on the image quality is analyzed for a dark-field microscopy imaging system, which is illuminated by a left-circularly (LC) polarized Laguerre–Gaussian (LG) vortex beam. To confirm the aberration tolerance range of the sharper focusing lens for precise measurement, the analytical expression of the field distribution on the image plane is deduced. Then the dark-field image results corresponding to a defect with a scale of 6µm×6µm are simulated. The influence of the spherical error, coma, and astigmatism on the imaging quality is compared in detail. Then the verification test has been carried out to show the effects of different primary aberrations. Finally, both the simulation and experiment results help us to identify that the tolerance range of spherical aberration, astigmatism, and coma should be less than 1.5λ, 1λ, and 1.25λ, respectively.

Screening and Identification of a Strain with Protease and Phytase Activities and Its Application in Soybean Meal Fermentation.

The aims of the study were to degrade the anti-nutritional factors (ANFs) such as phytic acid, glycinin, and β-conglycinin and improve the values of soybean meal (SBM). Firstly, in this study, a strain PY-4B which exhibited the best enzymatic activities of protease (403.3 ± 17.8 U/mL) and phytase (62.9 ± 2.9 U/mL) was isolated and screened among the isolates. Based on the analysis of physiological and biochemical characteristics and 16S rDNA sequence, the strain PY-4B was identified and named as Pseudomonas PY-4B. Next, Pseudomonas PY-4B was applied to fermentation of SBM. The results showed that the contents of glycinin and β-conglycinin were decreased by 57-63%, and the phytic acid was remarkably degraded by 62.5% due to the fermentation of SBM by Pseudomonas PY-4B. The degradation of glycinin and β-conglycinin resulted in increase of contents of water-soluble proteins and amino acids in fermented SBM. Moreover, Pseudomonas PY-4B exhibited no hemolytic activity and slight inhibitory effect on the growth of pathogen Staphylococcus aureus and the wide range of pH tolerance (3 to 9). In summary, our study indicates that isolated strain Pseudomonas PY-4B is a safe and applicable strain and has the ability to effectively degrade the ANFs (phytic acid, glycinin, and β-conglycinin) in SBM by fermentation.

Immobilization of α-galactosidase in polyvinyl alcohol-chitosan-glycidyl methacrylate hydrogels based on directional freezing-assisted salting-out strategy for hydrolysis of RFOs

Raffinose family oligosaccharides (RFOs) in food are the main factors causing flatulence in Irritable Bowel Syndrome (IBS) patients and the development of effective approaches for reducing food-derived RFOs is of paramount importance. In this study, polyvinyl alcohol (PVA)-chitosan (CS)-glycidyl methacrylate (GMA) immobilized α-galactosidase was prepared by the directional freezing-assisted salting-out technique, aimed to hydrolyze RFOs. SEM, FTIR, XPS, fluorescence and UV characterization results demonstrated that α-galactosidase was successfully cross-linked in the PVA-CS-GMA hydrogels, forming a distinct porous stable network through the covalent bond between the enzyme and the carrier. Mechanical performance and swelling capacity analysis illustrated that α-gal @ PVA-CS-GMA not only had suitable strength and toughness for longer durability, but also exhibited high water content and swelling capacity for better retention of catalytic activity. The enzymatic properties of α-gal @ PVA-CS-GMA showed an improved Km value, pH and temperature tolerance range, anti-enzymatic inhibitor (melibiose) activity compared to the free α-galactosidase and its reusability was at least 12 times with prolonged storage stability. Finally, it was successfully applied in the hydrolysis of RFOs in soybeans. These findings provide a new strategy for the development of α-galactosidase immobilization system to biological transform the RFOs components in the food for diet intervention of IBS.

Insulin desensitization and cell senescence induced by heat stress in pig testicular cell model

Thermostatic animals need to maintain a stable body temperature. A high-temperature environment can cause body temperature to exceed the range of tolerance of the organism, resulting in a heat stress response. The reproductive organs (such as the testes) are more sensitive to temperature due to their special anatomical location. However, to date, the effect of heat stress on the biological function of insulin in testicular cells has not been revealed. Therefore, the current study established a testis cell model to study the effect of heat stress on the biological activity of insulin. The results showed significant alterations in the insulin-induced intracellular signaling under heat stress conditions. Moreover, the IR-mediated intracellular signaling pathway was significantly downregulated under heat stress conditions. Further studies demonstrated that heat stress led to senescence of testicular cells by Sa-β-gal staining. Furthermore, the expression of senescence markers (p16 and p21) was increased under heat stress. In addition, heat stress was found to cause oxidative stress in testicular cells, which may be the underlying molecular mechanism by which heat stress changes the signaling properties of insulin. Collectively, the current study showed that heat stress caused alterations in insulin-induced intracellular signaling. Heat stress also induced testicular cell senescence.

Caribbean scleractinian corals exhibit highly variable tolerances to acute hypoxia

IntroductionClimate change, and the increase in sea surface temperature, is exacerbating ocean deoxygenation because of the inherent property of seawater to sequester less dissolved gas, such as oxygen, at warmer temperatures. While most coral reef studies focus on the effects of thermal stress and ocean acidification, few studies acknowledge the threat of hypoxia. Hypoxia is traditionally defined as 6.3 kPa (2 mg L-1 O2), however, a universal hypoxia threshold is not useful given the vast range of responses among marine organisms. The range of metabolic responses and tolerances to hypoxia are unknown for Caribbean coral species and their algal symbionts.ObjectiveHere, we quantified the spectrum of acute hypoxia tolerances and the range of metabolic responses of six ecologically and structurally important Caribbean coral species (Acropora cervicornis, Siderastrea radians, Siderastrea siderea, Porites astreoides, Porites porites, and Orbicella faveolata) and their algal symbionts (Symbiodinium, Breviolum, and Durusdinium spp.).MethodsA total of 24 coral fragments (4 individuals per species) were exposed to 10 distinct oxygen concentrations ranging from normoxia (20.38 kPa) to severe hypoxia (3.3 kPa). We used intermittent flow respirometry to measure coral host respiration in the dark and algal symbiont photosynthesis in the light at each oxygen level. We determined a line of best fit for the metabolic rate vs. PO2 data and calculated the critical oxygen partial pressure (PO2 crit), a method that has not been tested on symbiotic species.ResultsCoral species and their algal symbionts measured here displayed a wide range of hypoxia tolerances. For the coral hosts, PO2 crit values differed roughly two-fold ranging from 5.74 kPa to 16.93 kPa, and for the algal symbionts, PO2 crit values differed roughly three-fold ranging from 3.9 kPa to 11.3 kPa.DiscussionThese results should be regarded as a first step to characterizing the metabolic response and acute tolerance of multiple coral hosts and algal symbionts to a wide range of oxygen concentrations. Given that some PO2 crit values were above the generally accepted hypoxia threshold, these results have implications for the community composition of reefs under a rapidly changing climate and can guide purposeful reef restoration.

A comparative study of mean-line models based on enthalpy loss and analysis of a cavity-structured radial turbine for solar hybrid microturbine applications

Lightweight components are essential for the future of the energy and aerospace fields. This paper presents an innovative radial turbine design that utilizes a cavity structure on the rotor for solar hybrid microturbines. The performance of the cavity-structured radial turbine is studied using mean-line models, computational fluid dynamics (CFD), and finite element analysis (FEA) simulations. The radial turbine is compared using air and supercritical carbon dioxide (sCO2) as the working fluid. The mean-line models based on enthalpy losses from recent literature are validated using commercial code Rital and CFD results. The impact of the isentropic velocity ratio (νs) and absolute flow angle at the rotor inlet (α1) on the optimum design for the rotor radial turbine is also demonstrated. A comparative loss enthalpy breakdown on the rotor is examined, such as incidence loss, passage loss, trailing edge loss, clearance loss, exit loss, and windage loss. The results show that the recent mean-line models based on enthalpy losses can accurately estimate the stage efficiency for both air and sCO2 turbines. By comparing the simulation results obtained from 3D-CFD and mean-line models, the efficiency prediction deviation is within an acceptable range of tolerance for both turbines when comparing the results obtained from 3D-CFD and mean-line models. The FE analysis reveals that the maximum stress decreased significantly for the air and sCO2 turbine after the rotor blade was added using the cavity structure inside. The maximum stress value on the rotor with the cavity structure design is still below the yield strength of the material limits. Furthermore, after the variations of the cavity structure design were added to the rotor blade of both turbines, the results were promising when the dangerous resonance at below 4EO (Engine Order) was minimized. The results of this study provide a promising direction for the development of high-efficiency and lightweight turbines in the energy and aerospace fields.