Disease Tolerance Research Articles

The lived experience of Iranian adults from coronavirus disease 2019 (COVID-19)—A qualitative study

IntroductionThe widespread impact of the coronavirus disease 2019 (COVID-19) epidemic on human health causes sudden lifestyle changes, through social distancing and isolation at home, with social and economic consequences. This qualitative study aimed to identify the lived experiences of recovered adult patients from COVID-19 in Iran.MethodsThis qualitative research was conducted using a national phenomenological approach. The participants were patients who recovered from COVID-19 through their treatment period in the hospital or at home. Semi-structured in-depth interviews were applied for 45 participants who were invited using purposeful sampling and continued to achieve data saturation. The five-stage inductive process to analyze the structure of lived experience (IPSE) approach was used to analyze the data using MAXQDA 2020 software.ResultsAccording to the statements of the participants in the study, five types of experiences extracted during the period of suffering from the disease: nutritional problems, physical problems, the psychological burden caused by the disease, the supporting role of others in the disease tolerance, and the unpleasant and better experiences of the disease.DiscussionPatients with COVID-19 disease experience many physical and psychosocial consequences which affect their quality of life. Therefore, sociopsychological support provided by psychologists and family members can have ameliorating effects in reducing disease consequences. Further interventional studies were needed to capture these aspects of diseases.

High-Risk Plaque Characteristics in Patients with Suspected Stable Coronary Artery Disease and Impaired Glucose Tolerance: A Coronary Computed Tomography Angiography Study

Impaired glucose tolerance (IGT), a prediabetic state, is a known risk factor for coronary artery disease (CAD). Low-attenuation plaque (LAP) lesions are associated with a high risk of coronary events. We aimed to evaluate high-risk plaque characteristics in LAP lesions between patients with IGT and normal glucose tolerance (NGT) in patients suspected for stable CAD. Coronary computed tomography angiography (CCTA) identified LAP lesions and assessed plaque volumes, burdens, and high-risk plaque features. Glycemic tolerance was stratified using oral glucose tolerance tests. Among 148 patients, 202 LAP lesions were identified, with 93 patients classified as NGT and 55 as IGT. Patients with IGT had a significantly higher prevalence of LAP lesions compared with NGT (p = 0.007). LAP volume was higher in IGT (16.46 ± 12.52 mm3) compared with NGT (12.66 ± 9.72 mm3, p = 0.01), but this association did not persist in multivariate analysis. The LAP burden was greater in IGT (10.79 ± 6.84%) than NGT (8.62 ± 5.93%, p = 0.02), and the napkin-ring sign was more frequent in IGT (12%) versus NGT (5%, p = 0.02); these associations remained significant in multivariate analysis. Patients with IGT had a higher LAP burden and higher frequency of napkin-ring signs. These findings may help explain the common occurrence of prediabetes in patients with acute myocardial infarction.

Transcriptomic atlas reveals organ-specific disease tolerance in sickle cell mice.

Sickle cell disease (SCD) is the most common genetic disease in the world and a societal challenge. SCD is characterized by multi-organ injury related to intravascular hemolysis. To understand tissue-specific responses to intravascular hemolysis and exposure to heme, we present a transcriptomic atlas in the primary target organs of HbSS vs HbAA transgenic SCD mice. We explored the transcriptomes of liver, kidney, heart, lung and bone marrow from HbAA and HbSS Townes littermates at resting state and their changes after injection of heme, assessed by RNA sequencing. Inflammation and myeloid cell signatures were omnipresent in resting HbSS organs, liver being the most affected. The injection of heme triggered a robust inflammatory response in HbAA mice. Signatures of exposure to heme in HbAA mice were downstream of TLR4 (sensor of LPS but also of heme), IL1b, IL6 and IFNg, similarly to HbSS mice at rest. Nevertheless, HbSS mice were strikingly unresponsive to heme administration, irrespective of the organ. This tolerance was driven by upregulation of the heme-detoxifying enzyme HO-1 and was abrogated by its specific inhibition. Therefore, HbSS mice develop robust protective mechanisms, which may explain how they and SCD patients survive bouts of severe hemolysis.

Single-cell and spatial transcriptomics illuminate bat immunity and barrier tissue evolution.

Bats have adapted to pathogens through diverse mechanisms, including increased resistance - rapid pathogen elimination, and tolerance - limiting tissue damage following infection. In the Egyptian fruit bat (an important model in comparative immunology) several mechanisms conferring disease tolerance were discovered, but mechanisms underpinning resistance remain poorly understood. Previous studies on other species suggested that elevated basal expression of innate immune genes may lead to increased resistance to infection. Here, we test whether such transcriptional patterns occur in Egyptian fruit bat tissues through single-cell and spatial transcriptomics of gut, lung and blood cells, comparing gene expression between bat, mouse and human. Despite numerous recent loss and expansion events of interferons in the bat genome, interferon expression and induction are remarkably similar to that of mouse. In contrast, central complement system genes are highly and uniquely expressed in key regions in bat lung and gut epithelium, unlike in human and mouse. Interestingly, the unique expression of these genes in the bat gut is strongest in the crypt, where developmental expression programs are highly conserved. The complement system genes also evolve rapidly in their coding sequence across the bat lineage. Finally, the bat complement system displays strong hemolytic activity. Together, these results indicate a distinctive transcriptional divergence of the complement system, which may be linked to bat resistance, and highlight the intricate evolutionary landscape of bat immunity.

Multiple, Single Trait GWAS and Supervised Machine Learning Reveal the Genetic Architecture of Fraxinus excelsior Tolerance to Ash Dieback in Europe.

Common ash (Fraxinus excelsior) is under intensive attack from the invasive alien pathogenic fungus Hymenoscyphus fraxineus, causing ash dieback at epidemic levels throughout Europe. Previous studies have found significant genetic variation among genotypes in ash dieback susceptibility and that host phenology, such as autumn yellowing, is correlated with susceptibility of ash trees to H. fraxineus; however, the genomic basis of ash dieback tolerance in F. excelsior requires further investigation. Here, we integrate quantitative genetics based on multiple replicates and genome-wide association analyses with machine learning to reveal the genetic architecture of ash dieback tolerance and of phenological traits in F. excelsior populations in six European countries (Austria, Denmark, Germany, Ireland, Lithuania, Sweden). Based on phenotypic data of 486 F. excelsior replicated genotypes we observed negative genotypic correlations between crown damage caused by ash dieback and intensity of autumn leaf yellowing within multiple sampling sites. Our results suggest that the examined traits are polygenic and using genomic prediction models, with ranked single nucleotide polymorphisms (SNPs) based on GWAS associations as input, a large proportion of the variation was predicted by unlinked SNPs. Based on 100 unlinked SNPs, we can predict 55% of the variation in disease tolerance among genotypes (as phenotyped in genetic trials), increasing to a maximum of 63% when predicted from 9155 SNPs. In autumn leaf yellowing, 52% of variation is predicted by 100 unlinked SNPs, reaching a peak of 72% using 3740 SNPs. Based on feature permutations within genomic prediction models, a total of eight nonsynonymous SNPs linked to ash dieback crown damage and autumn leaf yellowing (three and five SNPs, respectively) were identified, these were located within genes related to plant defence (pattern triggered immunity, pathogen detection) and phenology (regulation of flowering and seed maturation, auxin transport). We did not find an overlap between genes associated with crown damage level and autumn leaf yellowing. Hence, our results shed light on the difference in the genomic basis of ADB tolerance and autumn leaf yellowing despite these two traits being correlated in quantitative genetic analysis. Overall, our methods show the applicability of genomic prediction models when combined with GWAS to reveal the genomic architecture of polygenic disease tolerance enabling the identification of ash dieback tolerant trees for breeding or conservation purposes.

β-Glucan reprograms neutrophils to promote disease tolerance against influenza A virus

Disease tolerance is an evolutionarily conserved host defense strategy that preserves tissue integrity and physiology without affecting pathogen load. Unlike host resistance, the mechanisms underlying disease tolerance remain poorly understood. In the present study, we investigated whether an adjuvant (β-glucan) can reprogram innate immunity to provide protection against influenza A virus (IAV) infection. β-Glucan treatment reduces the morbidity and mortality against IAV infection, independent of host resistance. The enhanced survival is the result of increased recruitment of neutrophils via RoRγt+ T cells in the lung tissue. β-Glucan treatment promotes granulopoiesis in a type 1 interferon-dependent manner that leads to the generation of a unique subset of immature neutrophils utilizing a mitochondrial oxidative metabolism and producing interleukin-10. Collectively, our data indicate that β-glucan reprograms hematopoietic stem cells to generate neutrophils with a new ‘regulatory’ function, which is required for promoting disease tolerance and maintaining lung tissue integrity against viral infection.

Use of Historical Cricket Scorecards to Accurately Map Effort Tolerance in Untreated Congenital Heart Disease.

This case report describes rare accurate historical documentation of progressive reduction in effort tolerance over time in a patient with untreated congenital heart disease and pulmonary hypertension.

Socioeconomic status of small ruminant rearers in selected provinces in Sierra Leone

This study focused on assessing the socio-economic characteristics of small ruminant farmers and identifying key challenges impairing the growth of the small ruminant local industry in selected provinces in Sierra Leone. The research adopted a cross-sectional design to generated primary information from 438 respondents in six selected districts using a well-structured pre-tested questionnaire. The data was analyzed using the statistical tool SPSS (version 23.0), the relationship between assessed variables and location were tested using Pearson’s Chi-square test. The findings of the study revealed that majority of the respondents were men (60.5%), married (83.8%), had no formal education (79.2%), and above 50 years old (62.8%). Small ruminants were predominantly owned by men, but women and children (47.5%) were mainly responsible for routine management practices. The primary reasons for keeping small ruminants include cultural practices (78.5%), household income (71.5%), food (66.3%), and religious practices (64.6%). Farmers preferred rearing small ruminants over other livestock due to high market demand (31.1%), disease tolerance (20.5%), adaptation (16.0%), and prolificacy (13.2%). Income generated from the sales of small ruminants was mostly used to purchase food (68.0%), for educational purposes (64.2%), and crop farming (51.6%). The major challenges for farmers were diseases/parasites (100.0%), inadequate animal health services (80.8%), animal theft (63.7%), and mortality (62.1%). According to 81.7%, 77.2%, 73.1%, and 71.2% of the farmers, peste des petits ruminants, skin infection, foot rot, and reproductive diseases were regarded as the main diseases of small ruminants. To enhance productivity of small ruminants in the study areas, continuous education, improved biosecurity, and robust measures by the government and community are required to overcome these challenges.

Genetic parameters and correlations between growth traits and packed cell volume of N'Dama cattle in the Gambia.

The evolution of body weight under the natural trypanosome challenge and its association with disease tolerance to trypanosomosis is of utmost economic importance in cattle. This study estimated heritability for growth traits and packed cell volume (PCV) and their genetic correlations in the N'Dama cattle in the Gambia. A total of 2,488, 2,442, 1,471, 1,934, and 1,452 bodyweight records at 12months (WT12), 16months (WT16), 18months (WT18), 24months (WT24), 36months (WT36) and 50months (WT50) and 1,782, 1,800, 1,844, 1,608, and 1,459 records for PCV at 12months (PCV12) 18months (PCV18), 24months (PCV24), 36months (PCV36), and 50months (PCV50), respectively, were analysed. Genetic parameters were estimated using univariate and multivariate animal models using the GIBBSF90 software. Least square means for WT12, WT18, WT24, WT36 and WT50 were 75.08 ± 0.37kg, 95.58 ± 0.52, 123.80 ± 0.52kg, 149.90 ± 0.73kg and 190.37 ± 0.68kg, respectively. The Least square means for PCV declined from 24.56 ± 0.18 at 12months of age to 23.18 ± 0.19 at 50months. Heritability estimates for growth ranged from 0.47 ± 0.05 at 12months to 0.31 ± 0.06 at 50months, while for PCV the estimates were 0.09 ± 0.01 to 0.15 ± 0.01. Genetic correlations between bodyweights were high and positive (0.73 ± 0.01 to 0.96 ± 0.01) while those between PCV traits were low to medium and positive (0.16 ± 0.04 to 0.56 ± 0.02). Genetic correlations between bodyweight and PCV range from -0.14 ± 0.02 to 0.59 ± 0.02. BW18 and PCV18 had high heritability estimates and as well as the highest genetic correlation and therefore could be used as selection criteria for body weight and trypanotolerance, respectively.

Supplemental n-3 polyunsaturated fatty acids: Clinical implications and best practices for adult patients with cancer

Abstract Background The purpose of this report is to summarize the evidence supporting supplementation of n-3 polyunsaturated fatty acids (n-3 PUFAs) in adult cancer patients, and to offer a better understanding of the appropriate use of n-3 PUFAs in the clinical setting. Methods Numerous databases were searched for guidelines, clinical decision-making documents, systematic reviews, expert consensus statements, and best evidence summaries about the use of n-3 PUFAs in cancer patients from the inception of the database to December 31, 2023. Evidence grading and recommendation rating were conducted. The data extracted included the timing of supplementation, symptom management, disease prevention, cost-effectiveness, route of administration, application scenarios, dosage, and safety. Results The collected data show that n-3 PUFAs are safe for patients receiving chemotherapy who are at risk of malnutrition and cachexia. Moreover, n-3 PUFA supplementation can alleviate the adverse symptoms associated with chemotherapy, extend survival, and improve the quality of life of patients with cancer. Conclusions The administration of supplementary n-3 PUFAs should be considered based on the patient’s disease stage, treatment plan, nutritional status, and tolerance, as well as the dosage, route and application scenarios. Promoting the clinical use of n-3 PUFAs may improve the outcomes for patients with cancer.

Breeding potential of eggplant genotypes for mosaic virus disease tolerance using multivariate analysis

ABSTRACT Eggplant (Solanum melongena L.) cultivation is currently threatened in the tropics and subtropics, leading to enormous economic losses. Identifying genetically diverse donor parents for the development of mosaic-resistant varieties/hybrids in disease hotspot areas needs to be improved. Twenty-nine eggplant genotypes were screened for tolerance to mosaic disease and other economic traits to identify genotypes that can be utilized in future breeding. Based on field screening, the genotypes ‘2019/BRR Var-11,’ ‘2020/BRR Var-7,’ ‘2020/BRL Var-1,’ and ‘2020/BRL Var-5’ were found to be moderately resistant (>10%–20% disease severity) to mosaic disease, according to the disease rating scale. This study revealed no parallelism between genetic diversity and geographical divergence in eggplant. Multivariate analysis and the mean of economic characters indicate that the genotypes ‘2020/BRR Var-7,’ ‘2019/BRR Var-11,’ ‘2020/BRL VAR-1,’ ‘2020/BRL VAR-5,’ ‘2019/BRR Var-7,’ and ‘2019/BRL Var-7’ could be potential donors for mosaic disease resistance breeding. Phenylalanine ammonia-lyase, peroxidase, polyphenol oxidase, total phenols, and trichome density in the abaxial and adaxial leaves exhibited significant negative correlations with mosaic disease incidence, suggesting their application as selection indices for the identification of tolerant genotypes. Breeders might be able to introgress mosaic disease tolerance into commercial genotypes through systemic breeding to obtain prospective recombinants possessing desired economic characteristics.

Extensive longevity and DNA virus-driven adaptation in nearctic Myotis bats.

The genus Myotis is one of the largest clades of bats, and exhibits some of the most extreme variation in lifespans among mammals alongside unique adaptations to viral tolerance and immune defense. To study the evolution of longevity-associated traits and infectious disease, we generated near-complete genome assemblies and cell lines for 8 closely related species of Myotis. Using genome-wide screens of positive selection, analyses of structural variation, and functional experiments in primary cell lines, we identify new patterns of adaptation contributing to longevity, cancer resistance, and viral interactions in bats. We find that Myotis bats have some of the most significant variation in cancer risk across mammals and demonstrate a unique DNA damage response in primary cells of the long-lived M. lucifugus. We also find evidence of abundant adaptation in response to DNA viruses - but not RNA viruses - in Myotis and other bats in sharp contrast with other mammals, potentially contributing to the role of bats as reservoirs of zoonoses. Together, our results demonstrate how genomics and primary cells derived from diverse taxa uncover the molecular bases of extreme adaptations in non-model organisms.

High-affinity chimeric antigen receptor signaling induces an inflammatory program in human regulatory T cells

Regulatory Tcells (Tregs) are promising cellular therapies to induce immune tolerance in organ transplantation and autoimmune disease. The success of chimeric antigen receptor (CAR) Tcell therapy for cancer has sparked interest in using CARs to generate antigen-specific Tregs. Here, we compared CAR with endogenous Tcell receptor (TCR)/CD28 activation in human Tregs. Strikingly, CAR Tregs displayed increased cytotoxicity and diminished suppression of antigen-presenting cells and effector T (Teff) cells compared with TCR/CD28-activated Tregs. RNA sequencing revealed that CAR Tregs activate Teff cell gene programs. Indeed, CAR Tregs secreted high levels of inflammatory cytokines, with a subset of FOXP3+ CAR Tregs uniquely acquiring CD40L surface expression and producing IFN-γ. Interestingly, decreasing CAR antigen affinity reduced Teff cell gene expression and inflammatory cytokine production by CAR Tregs. Our findings showcase the impact of engineered receptor activation on Treg biology and support tailoring CAR constructs to Tregs for maximal therapeutic efficacy.

High Yielding Pest and Disease Tolerance Variety (DHLM-14-1) in Little Millet (Panicum sumetrense. L) Developed through Recombinant Breeding Technology

The little millet variety DHLM-14-1 developed at the Agricultural Research Station (ARS) Hanumanamatti under the University of Agricultural Sciences, Dharwad. With a maturation period of 85-90 days, this variety features an erect, tall plant stature ranging from 112 to 130 cm and produces bold, oval-shaped gray grains. DHLM-14-1 stands out for its notable tolerance to shoot fly, exhibiting only a 13.89% incidence compared to 19.15% in the national check JK-8. Its impressive yield performance is evident, yielding 18.42%, 30.58%, and 3.14% more than the national checks OLM-203, JK-8, and KOPLM-53, respectively, making it a beneficial option for millet producers looking for resilient, high-yielding crops. Across trials conducted from 2011 to 2014, DHLM-14-1 showcased exceptional agronomic performance, achieving a mean seed yield of 42.41q/ha, significantly outperforming local check TNAU-63 and national check OLM-203 by 26.63% and 23.75%, respectively. Its consistent yield superiority, with an average of 15.89 q/ha over three years, illustrates the cultivar's adaptability to diverse agro-climatic conditions, surpassing OLM-203 and JK-8 by 18.42% and 30.58%. Recognized during the 29th Annual Group Meeting of the ICAR All India Coordinated Research Project on small millets, DHLM-14-1 has shown strong disease resistance against grain smut, brown spot, and sheath blight, with disease incidences similar to OLM-203 and significantly lower than JK-8. Additionally, its effective resistance to shoot fly underscores its potential to reduce pest damage, reinforcing DHLM-14-1's value as a high-yielding, resilient cultivar that not only enhances sustainable millet production but also supports food security and improves farmer livelihoods across India.

Extent and patterns of morphological and molecular genetic diversity and population structure of Nigerian Taro cultivars

BackgroundGenetic diversity is crucial for conservation efforts as well as breeding programs targeted at the development of improved varieties. Taro, a climate-resilient crop, plays a vital role in the nutritional and economic livelihoods of many households in Nigeria, but its yield is very low due to inadequate genetic improvement efforts. A diversity assessment of Nigerian taro is therefore required to create a premise for its improvement in yield, quality and disease tolerance. In this study, the genetic diversity and population structure of 490 taro cultivars comprising two main gene pools: Dasheen (215) and Eddoe (275), collected from farmers and marketers across seven states in Nigeria was assessed using 3047 Diversity Array Technology single nucleotide polymorphism (DArT-SNP) markers. A subset of 114 taro cultivars, comprising 30 Dasheens and 84 Eddoes were further phenotyped using 24 agro-morphological descriptors.ResultsBoth phenotypic and molecular characterization revealed higher genetic diversity among the Eddoes than Dasheens. Estimates of gene flow (Nm = 0.353) revealed intermixing of cultivars among the States of collection, with the highest gene flow occurring between cultivars from Anambra and Ondo states and the lowest between Anambra and Kwara states. Population structure and Ward’s minimum variance hierarchical cluster based on DArT-SNPs identified four groups, one comprising Dasheen and three comprising Eddoe cultivars. Hierarchical clustering based on phenotypic traits delineated three clusters. Variation between gene pools (49%) was higher than within gene pools (32%). Variation among States of collection was high (41%), while variation among individuals within gene pools (18%) and States of collection (19%) was relatively low. Correlation between phenotypic and genotypic diversity assessments was low (r = 0.01), indicating that both approaches were necessary for assessing genetic diversity in taro. However, genotypic assessment provided better information about genetic diversity of the taro cultivars.ConclusionThis is the first study that represented germplasm collection across the major taro growing regions of Nigeria. The findings from this study based on agro-morphological characterization and DArT-SNP genotyping are critical for genetic characterization, conservation and breeding of taro in Nigeria, mainly initiating hybridization between the two genepools after careful assessment of ploidy levels of the accessions collected in this study. This will facilitate in developing improved taro varieties with desirable traits, such as higher yield, better disease resistance, and improved nutritional quality.

Breeding for Pest and Disease Tolerance High Yielding Variety (DHLM-36-3-1) in Little Millet (Panicum sumetrense. L)

Developed from 2008 to 2013 and evaluated between 2010 and 2014, the high-yielding medium-maturing little millet variety DHLM-36-3-1 was officially released and notified in 2018, showing its potential as an excellent variety. This cultivar, developed at the Agricultural Research Station in Hanumanamatti, University of Agricultural Sciences, Dharwad, is well-suited to the state of Karnataka. It matures in 85 to 90 days and features an erect growth habit, reaching heights of 112 to 130 cm, with bold, oval-shaped grains of light black color. DHLM-36-3-1 demonstrated a grain yield of 14.29 q/ha and a fodder yield of 5.6 t/ha, showing good tolerance to shoot fly infestation (15.50%) compared with the national check JK-8, which recorded a 20.34% infestation rate. Furthermore, DHLM-36-3-1 outperformed both national checks OLM-203 and JK-8 by 9.59% and 11.21%, respectively, in terms of grain yield. Similarly, the newly developed variety DHLM-14-1 has yielded promising results across various trials conducted throughout India, demonstrating its potential as a high-yielding and nutritionally rich option for farmers. Over three growing seasons, DHLM-14-1 achieved an impressive average seed yield of 35.12 q/ha, exceeding the local check TNAU-63 by 18.56% and the national check OLM-203 by 12.06%. It has consistently performed well in All India Coordinated Trials, showing adaptability across diverse agro-climatic conditions, with notable yield improvements in states such as Andhra Pradesh, Jharkhand, and Tamil Nadu. Nutritional assessments highlight DHLM-14-1's superior mineral profile, boasting higher levels of zinc (4.06 mg/100 g) and calcium (3.45 mg/100 g) compared to national checks, while its iron content (16.2 mg/100 g) significantly surpasses that of OLM-203. Additionally, disease resistance evaluations revealed that DHLM-14-1 maintained lower incidence rates for grain smut and brown spots, with competitive resistance to shoot fly. Collectively, these findings emphasize DHLM-14-1's vital role in promoting sustainable millet production, enhancing food security and farmer livelihoods, supporting integrated pest management strategies, and improving the nutritional quality of staple diets in areas with micronutrient deficiencies.

Generating bat primary and immortalised cell-lines from wing biopsies

Bats are becoming recognised as new model species to study naturally evolved mammalian extended healthspan and disease tolerance. However, this research is limited by the lack of bat specific cellular resources. Here we describe an optimised protocol to develop both primary and immortalised fibroblast cell-lines from wing biopsy punches from the Egyptian fruit bat, Rousettus aegyptiacus. We show that the immortalised cell lines and primary cells show similar characteristics in their proliferative capacity and response to oxidative stress. They also exhibited a similar response in their NF-κB immune response to TLR agonists including SARS-CoV2. As wing punches can be acquired non-lethally, these methods can be used to develop primary and immortalised cells, from potentially any bat species, including those of conservation concern that cannot be sacrificed. This can expand the scope of bat species that can be studied in the future, and the development of key cellular resources required to functionally validate the regulators of bats’ unique longevity.

A naturally occurring mitochondrial genome variant confers broad protection from infection in Drosophila.

The role of mitochondria in immunity is increasingly recognized, but it is unclear how variation in mitochondrial DNA (mtDNA) contributes to variable infection outcomes. To quantify the effect of mtDNA variation on humoral and cell-mediated innate immune responses, we utilized a panel of fruit fly Drosophila melanogaster cytoplasmic hybrids (cybrids), where unique mtDNAs (mitotypes) were introgressed into a controlled isogenic nuclear background. We observed substantial heterogeneity in infection outcomes within the cybrid panel upon bacterial, viral and parasitoid infections, driven by the mitotype. One of the mitotypes, mtKSA2, protected against bacterial, parasitoid, and to a lesser extent, viral infections. Enhanced survival was not a result of improved bacterial clearance, suggesting mtKSA2 confers increased disease tolerance. Transcriptome sequencing showed that the mtKSA2 mitotype had an upregulation of genes related to mitochondrial respiration and phagocytosis in uninfected flies. Upon infection, mtKSA2 flies exhibited infection type and duration specific transcriptomic changes. Furthermore, uninfected mtKSA2 larvae showed immune activation of hemocytes (immune cells), increased hemocyte numbers and ROS production, and enhanced encapsulation response against parasitoid wasp eggs and larvae. Our results show that mtDNA variation acts as an immunomodulatory factor in both humoral and cell-mediated innate immunity and that specific mitotypes can provide broad protection against infections.

Advancing Vegetable Grafting: A Comprehensive Review of Techniques, Challenges, and the Future of Automated Solutions

Vegetable grafting, a practice that combines two plant parts rootstock and scion into a single, superior plant, is an increasingly important technique in modern agriculture. By enhancing yields, stress resistance, and disease tolerance, grafting plays a crucial role in addressing challenges such as soil borne pathogens, extreme environmental conditions, and crop vulnerability to nematodes. Despite its benefits, vegetable grafting faces several challenges, including the need for compatible plant material, labour-intensive nursery production, high seed costs, and the potential for pathogen spread. Effective grafting requires meticulous selection of rootstock and scion, precise grafting techniques, and optimal post-grafting care to ensure survival and success. Looking toward the future, significant advancements in grafting technologies and nursery practices are required to overcome these challenges and improve efficiency. The development of grafting robots, capable of mechanizing the grafting process, holds promise for increasing productivity while reducing labor costs. Additionally, the establishment of specialized plug plantlet nurseries, equipped with advanced seeders, growth chambers, and acclimatization facilities, offers a pathway to enhance seedling vigour and uniformity in developing countries. Future research should also focus on refining seed-priming methods, improving seedling storage technologies, and exploring digital tools such as databases, crop models, and mobile applications to optimize grafting practices. These innovations could provide valuable insights for growers, helping them select the best rootstock-scion combinations and manage crops more efficiently. With continued research and technological advancements, vegetable grafting is poised to become a global solution, offering sustainable growth and productivity improvements in agriculture.

Deciphering Phyllomicrobiome of Cauliflower Leaf: Revelation by Metagenomic and Microbiological Analysis of Tolerant and Susceptible Genotypes Against Black Rot Disease.

Understanding the phyllomicrobiome dynamics in cauliflower plants holds significant promise for enhancing crop resilience against black rot disease, caused by Xanthomonas campestris pv. campestris. In this study, the culturable microbiome and metagenomic profile of tolerant (BR-161) and susceptible (Pusa Sharad) cauliflower genotypes were investigated to elucidate microbial interactions associated with disease tolerance. Isolation of phyllospheric bacteria from asymptomatic and black rot disease symptomatic leaves of tolerant and susceptible cultivars yielded 46 diverse bacterial isolates. Molecular identification via 16S rRNA sequencing revealed differences in the diversity of microbial taxa between genotypes and health conditions. Metagenomic profiling using next-generation sequencing elucidated distinct microbial communities, with higher diversity observed in black rot disease symptomatic leaf of BR-161. Alpha and beta diversity indices highlighted differences in microbial community structure and composition between genotypes and health conditions. Taxonomic analysis revealed a core microbiome consisting of genera such as Xanthomonas, Psychrobacillus, Lactobacillus, and Pseudomonas across all the samples. Validation through microbiological methods confirmed the presence of these key genera. The findings provide novel insights into the phyllomicrobiome of black rot-tolerant and susceptible genotypes of cauliflower. Harnessing beneficial microbial communities identified in this study offers promising avenues for developing sustainable strategies to manage black rot disease and enhance cauliflower crop health and productivity.