Muscle Tissue Research Articles

Modulatory effect of Echium plantagineum oil on the n-3 LC-PUFA biosynthetic capacity of chicken (Gallus gallus).

Poultry can be a sustainable source of eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) through the bioconversion of dietary alpha-linolenic acid (ALA, 18:3n-3). However, this process is currently limited by the high n-6/n-3 ratio in poultry diets affecting the competition between n-6 and n-3 fatty acids (FA) for the same biosynthetic enzymes, and the rate-limiting Δ6 desaturase which act at both, the first and final steps of DHA synthesis pathway. Echium plantagineum oil (EO) is an unusual source of stearidonic acid (SDA, 18:4n-3) which bypasses the first Δ6 desaturase step potentially increasing n-3 long-chain polyunsaturated fatty acids (LC-PUFA) synthesis. To explore this hypothesis, 60 Canarian male chickens at 18 weeks of age were divided into three groups and fed diets differing only in their FA formulation: soy oil (SO) rich in linoleic acid (LA, 18:2n-6); linseed oil (LO) rich in ALA; and EO, a balanced LA/ALA oil also rich in SDA and γ-linolenic acid (GLA, 18:3n-6). The dietary treatments did not affect the total lipid (TL) content (p>0.05) and did not substantially vary the lipid class (LC) profiles in the brain, liver, intestine, and muscle tissues. However, the inclusion of LO and EO equally increased n-3 polyunsaturated fatty acids (PUFA) levels in the brain, liver, and intestine compared to animals fed with SO (p<0.05). Moreover, EO increased hepatic relative expressions of the fatty acid elongases (elovl2 and elovl5). Consequently, and in alignment with our hypothesis, EO was more effective than LO in enriching chicken thigh meat with n-3 LC-PUFA (6.0 vs 4.2%; p<0.05). We concluded that lowering the dietary LA/ALA ratio and increasing the SDA content in poultry diets enhance the potential of chicken metabolism for enriching poultry products with n-3 LC-PUFA. Emerging evidence suggest that local plants like those including in Echium genus, rich in SDA and with a balanced LA/ALA ratio, could offer a more sustainable and efficient alternative to traditional ALA sources in poultry production.

Conservative Tangential Excision Instead of Escharotomy in the treatment of Compartment Syndrome

Purpose: One of the primary causes of heat-induced compartment syndrome is the loss of elasticity of the body surface eschar itself, we have recently replaced escharotomy with conservative tangential excision in the treatment of compartment syndrome caused by heat and achieved good results.Methods: Since 2019, our burn center has applied conservative tangential excision and heterogeneous dermal coverage technology for decompression treatment of 16 patients with thermal-induced compartment syndrome, involving 19 parts of the limbs and 5 parts of the chest and abdomen. All cases were treated with decompression under general anesthesia within 12-48 hours after burn.Results: All patients were treated with conservative tangential excision for decompression. After limb decompression, the peripheral blood supply was restored, and the obvious pulse could be touched. No further fasciotomy was performed, and no secondary damage of deep muscle, nerve and vascular tissue was found in the follow-up treatment; After thoracic and abdominal decompression, there was no respiratory restriction and circulatory disorder.Conclusion: Conservative tangential excision represents an effective method for both the prevention and treatment of heat-induced compartment syndrome, it can be used as an alternative method for escharotomy.

Reconstructive techniques for chest and breast deformities in Poland syndrome: An up-to-date systematic review.

Poland syndrome (PS) is a congenital abnormality defined as aplasia or hypoplasia of the unilateral pectoralis muscle and breast tissue that may be accompanied by limb or thoracic deformities. Reconstruction of deformities associated with PS is challenging owing to the spectrum of differences. We aimed to evaluate the trends in surgical management of chest and breast anatomical anomalies associated with PS. A systematic review of studies describing primary surgical management of chest and breast deformities in PS was conducted. Papers exclusively describing PS-associated hand reconstruction or intrathoracic surgeries were excluded. Among the 741 articles, 77 met inclusion criteria. Flap-based reconstruction was used in 60.3% of cases, predominantly involving the latissimus dorsi muscle (60.3%). Tissue expanders were used in 22.5% of the patients, all of whom were women. Among them, 80.8% eventually underwent implant-based reconstruction. Among the male patients, flaps alone were the most common reconstructive modality (41.7%), whereas the predominant approach for women was hybrid reconstruction using flaps and implants (34.2%). In temporal analyses, the use of fat grafting, either alone or as an adjunct to other reconstructive techniques, has increased since the 1990s. Reconstructive strategies employed in male and female patients with PS are varied, likely owing to the differences in the desired breast contour. There is no gold standard for the correction of chest and breast deformities associated with PS; however, new techniques such as fat grafting and hybrid reconstruction are gaining popularity as viable reconstructive options. We present a decisional framework to best address the varying anomalies associated with PS in men and women.

Transcriptomic and proteomic profiling identifies feline fibrosarcoma as clinically amenable model for aggressive sarcoma subtypes.

Fibrosarcomas (FSA) are malignant mesenchymal tumors characterized by low chemo- and radiosensitivity. Development of novel treatment strategies for human adult FSA is hindered by the low incidence and the absence of suitable clinical models. Interestingly, aggressive FSA occur more frequently in domestic cats, hence potentially representing a clinically amenable model to assess novel therapies such as targeted imaging or theranostics. However, a lack of molecular characterization of FSA and adjacent normal tissue (NT) in both species hinders identification of tumor-specific targets and undermines the translational potential of feline FSA. Combining laser-capture microdissection, RNAsequencing and liquid chromatography-tandem mass spectrometry, we perform comprehensive profiling of 30 feline FSA and matched skeletal muscle, adipose and connective tissue. Clear inter-tissue differences allow identification of significantly upregulated and tumor-exclusive features that represent potential targets for diagnostic and therapeutic approaches. While feline FSA are characterized by hyperactive EIF2, TP53 and MYC signaling, immune-related and neuronal pathways emerge as modulators of tumor aggressiveness and immunosuppression. A high degree of molecular similarity with canine and adult FSA allows identification of tumor targets that are conserved across species. Significant enrichment in DNA repair pathways in feline FSA correlate with aggressive clinical behavior in human soft-tissue sarcoma. Finally, we leverage the molecular profiles to identify vulnerabilities, including sensitivity to ATR and PARP inhibition as potential treatment for feline FSA. In conclusion, this detailed landscape provides a rich resource to identify target candidates and therapeutic vulnerabilities within and across species and supports feline FSA as relevant models for the human disease.

Characterization of SARS-CoV-2 Entry Genes in Skeletal Muscle and Impacts of InVitro Versus InVivo Infection.

COVID-19 has been associated with both respiratory (diaphragm) and non-respiratory (limb) muscle atrophy. It is unclear if SARS-CoV-2 infection of skeletal muscle plays a role in these changes. This study sought to: 1) determine if cells comprising skeletal muscle tissue, particularly myofibres, express the molecular components required for SARS-CoV-2 infection; 2) assess the capacity for direct SARS-CoV-2 infection and its impact on atrophy pathway genes in myogenic cells; and 3) in an animal model of COVID-19, examine the relationship between viral infection of skeletal muscle and myofibre atrophy within the diaphragm and limb muscles. We used in silico bioinformatics analysis of published human single cell RNA-seq datasets, as well as direct qPCR examination of human myotubes and diaphragm biopsies, to assess expression of key genes involved in SARS-CoV-2 cellular entry. InVitro, we determined the ability of SARS-CoV-2 to directly infect myogenic cells and employed qPCR to assess the impact on muscle atrophy pathway genes (ubiquitin-proteasome, autophagy). Invivo, the diaphragm and quadriceps of Roborovski hamsters with SARS-CoV-2 respiratory infection were examined at day 3 post-inoculation to evaluate the relationship between atrophy pathway and SARS-CoV-2 transcripts by qPCR, as well as histological measurements of myofibre morphology. Angiotensin converting enzyme 2 (ACE2), the primary receptor for SARS-CoV-2, as well as cooperating proteases (furin, cathepsins B and L), are expressed by myofibres. ACE2 expression was increased 5-fold (p = 0.01) in the diaphragms of mechanically ventilated human subjects compared to controls. InVitro, a time-dependent increase of SARS-CoV-2 transcript levels was observed in myotubes directly exposed to the virus (p = 0.002). This was associated with downregulation of the ubiquitin ligase MuRF1 (by 64%, p = 0.002) and the autophagy gene LC3B (by 31%, p = 0.009). In contrast, invivo infection led to upregulation of MuRF1 in quadriceps (23-fold, p = 0.0007) and autophagy genes in both quadriceps (5.2-fold for Gabarapl1, p = 0.03; 7-fold for p62, p = 0.0002) and diaphragm (2.2-fold for Gabarapl1, p = 0.03; 2.3-fold for p62, p = 0.057). In infected hamsters the diaphragm lacked viral transcripts but exhibited atrophy (48% decrease in myofibre area; p = 0.02), whereas the quadriceps lacked myofibre atrophy despite elevated viral transcripts in the muscle. Although myogenic cells express the genes required for SARS-CoV-2 entry and can be directly infected, there was no evident relationship between viral transcript levels and manifestations of atrophy, either invitro or invivo. Our results do not support direct myofibre infection by SARS-CoV-2 as a likely cause of atrophy in COVID-19.

Key Pathophysiological Role of Skeletal Muscle Disturbance in Post COVID and Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): Accumulated Evidence.

Recent studies provide strong evidence for a key role of skeletal muscle pathophysiology in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). In a 2021 review article on the pathophysiology of ME/CFS, we postulated that hypoperfusion and ischemia can result in excessive sodium and calcium overload in skeletal muscles of ME/CFS patients to cause mitochondrial damage. Since then, experimental evidence has been provided that supports this concept. We collect, summarize and discuss the current state of knowledge for the key role of skeletal muscle pathophysiology. We try to explain which risk factors and mechanisms are responsible for a subgroup of patients with post COVID syndrome (PCS) to develop ME/CFS (PC-ME/CFS). Mitochondrial dysfunction is a long-held assumption to explain cardinal symptoms of ME/CFS. However, mitochondrial dysfunction could not be convincingly shown in leukocytes. By contrast, recent studies provide strong evidence for mitochondrial dysfunction in skeletal muscle tissue in ME/CFS. An electron microscopy study could directly show damage of mitochondria in skeletal muscle of ME/CFS patients with a preferential subsarcolemmal localization but not in PCS. Another study shows signs of skeletal muscle damage and regeneration in biopsies taken one day after exercise in PC-ME/CFS. The simultaneous presence of necroses and signs of regeneration supports the concept of repeated damage. Other studies correlated diminished hand grip strength (HGS) with symptom severity and prognosis. A MRI study showed that intracellular sodium in muscles of ME/CFS patients is elevated and that levels correlate inversely with HGS. This finding corroborates our concept of sodium and consecutive calcium overload as cause of muscular and mitochondrial damage caused by enhanced proton-sodium exchange due to anaerobic metabolism and diminished activity of the sodium-potassium-ATPase. The histological investigations in ME/CFS exclude ischemia by microvascular obstruction, viral presence or immune myositis. The only known exercise-induced mechanism of damage left is sodium induced calcium overload. If ionic disturbance and mitochondrial dysfunction is severe enough the patient may be captured in a vicious circle. This energy deficit is the most likely cause of exertional intolerance and post exertional malaise and is further aggravated by exertion. Based on this pathomechanism, future treatment approaches should focus on normalizing the cause of ionic disbalance. Current treatment strategies targeting hypoperfusion have the potential to improve the dysfunction of ion transporters.

Assessment of heavy metals concentration and health risk in four species of Persian Gulf sharks in Hormozgan Province.

Marine pollution is a growing concern that threatens ecosystems and endangers human health. This study investigated the bioaccumulation of six heavy metals, including zinc (Zn), copper (Cu), lead (Pb), mercury (Hg), nickel (Ni), and cadmium (Cd) in the muscle tissue of Codium arabicum, Coilia dussumieri, Rhizoprionodon oligolinx, and R. acutus from in Hormozgan province, Iran, between November 2023 and March 2024. The primary focus was to provide insights into these elements' distribution and accumulation patterns in Persian Gulf sharks. Moreover, it aimed to assess the potential risks of chemical contamination in the Persian Gulf. Samples were collected, prepared through acid digestion, and analyzed using Atomic absorption spectrometry (AAS) to qualitatively and quantitatively determine Zn, Cu, Ni, Pb, Cd, and Hg concentrations. The mean heavy metal concentrations in the examined sharks follow the order Zn > Cu > Ni > Pb > Cd > Hg. The highest mean concentrations of all the tested heavy metals were observed in C. arabica. Furthermore, the THQ value for Hg (1.07) in C. arabica raised concerns, particularly for individuals with high fish consumption, such as fishermen. With the THQ value exceeding 1, there is a clear need for heightened concern regarding the carcinogenic risk associated with dietary mercury exposure. Consequently, our findings showed the initial account of metal concentrations in Persian Gulf sharks, indicating elevated concentrations in individuals that may lead to sub-lethal effects. In conclusion, these results emphasized the potential toxicity of C. arabica meat and held significant implications for human consumers.

Monitoring and Assessment of Heavy Metal Concentrations in Two Black Fish Species, Capoeta saadii (Heckel, 1847) and Capoeta trutta (Heckel, 1843), in Western Iran.

Contamination of aquatic ecosystems with heavy metals poses a significant global issue due to its hazardous effects and persistent accumulation in living organisms. This study analyzed 51 fish samples from two species of Black Fish, Capoeta saadii and Capoeta trutta, collected from Iran's Khorramroud River during the summer and fall of 2022 to assess heavy metal accumulation in their gill, liver, and muscle tissues. After biometry, the studied tissues of each fish were isolated to measure the concentration of heavy metals (cadmium (Cd), zinc (Zn), chromium (Cr), lead (Pb), copper (Cu), and nickel (Ni)). The tissue concentration of the studied metals was obtained by measuring in the inductively coupled plasma optical emission spectroscopy (ICP-OES) device after chemical digestion. Based on the results of this study, muscle and liver tissues had the lowest and highest accumulation of heavy metals, respectively, with zinc concentration in the liver of C. saadii reaching 118.557 mg/kg, which exceeds the permissible limit of 75 mg/kg. Also, there was no significant correlation between the amount of absorption of heavy elements and the biometric indicators of body weight and length. Although C. saadii accumulated higher levels of Zn, Cu, Cd, and Cr than C. trutta, statistical analysis (ANOVA and PCA) revealed no significant differences in heavy metal accumulation between the two species (P > 0.05). Comparisons with international standards indicated that chromium (2.10-9.97 mg/kg) and zinc concentrations (41.72-118.557 mg/kg) exceeded permissible limits, while Ni, Cu, Pb, and Cr levels remained below the allowable thresholds. These findings underscore the need for strategic planning, management, and continuous environmental monitoring to mitigate heavy metal pollution in aquatic ecosystems and safeguard the health of fish populations in the Khorramroud River.

Overexpression of Drosophila NUAK or Constitutively-Active Formin-Like Promotes the Formation of Aberrant Myofibrils.

Muscle development and maintenance is central to the normal functioning of animals. Muscle tissues exhibit high levels of activity and require the dynamic turnover of proteins. An actomyosin scaffold functions with additional proteins comprising the basic contractile subunit of striated muscle, known as the sarcomere. Drosophila muscles are similar to vertebrate muscles in composition and they share a similar mechanism of development. Drosophila NUAK (NUAK) is the homolog of NUAK1 and NUAK2 in vertebrates. NUAK belongs to the family of AMP-activated protein kinases (AMPKs), a group of proteins with broad and overlapping cellular targets. Here we confirm that NUAK dynamically modulates larval muscle sarcomere size as upregulation of NUAK produces longer sarcomeres, including increased thin filament lengths. Furthermore, NUAK overexpression results in aberrant myofibers above the nuclei plane, upregulation of Formin-like (Frl), and an increase in newly synthesized proteins at sites consistent with actin filament assembly. Expression of constitutively-active Frl also produces aberrant myofibers similar to NUAK overexpression. These results taken together strongly suggest a functional link between NUAK and Frl in myofibril formation in an invivo setting.

Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue

Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue

ВЛИЯНИЕ ПРОЦЕССОВ ХРАНЕНИЯ И ТЕРМООБРАБОТКИ ЧЕРНОГО МАКРУРУСА НА ПОКАЗАТЕЛИ КАЧЕСТВА РЫБЫ-СЫРЦА И ГОТОВОГО ПРОДУКТА

The purpose of research is to study the dependence of the quality of raw and finished products from a promising for fishing and processing deep-sea fish species – black grenadier Coryphaenoides acrolepis – on processing and storage conditions. The fish was caught in the South Kuril subzone of the Sea of Okhotsk at a depth of 1000 m. Tasks: determination of the size-mass composition, functional-technological and physical and chemical properties of muscle tissue of two size groups of this species, the study of the rheological parameters of raw fish before and after heat treatment at various cutting and storage methods, as well as repeated freezing / thawing. The study was conducted in the laboratory of the Research Institute of Innovative Biotechnologies of the Far Eastern State Technical Fisheries University in Vladivostok. The following indicators were used as the main criteria for evaluating the functional and technological properties of fish muscle tissue: watering coefficient (Ko); protein-water ratio (BVK); structure formation coefficient (Kst); conditional protein coefficient (Kb); lipid-protein coefficient (Kf). The absence of significant differences in all studied parameters in two size groups before and after heat treatment is shown. Storage of carcasses and fillets in a catering establishment for 3 months to a greater extent affected the quality of carcasses in the direction of increasing the strength of muscle tissue. Repeated multiple freezing, used to search for the cause of the appearance of specimens with a rubbery consistency of muscle tissue, showed the absence of causal relationships of these processes and was confirmed by studies of changes in the molecular weight distribution of protein fractions of muscle tissue before and after storage by polyacrylamide gel electrophoresis.

P-59 A CASE REPORT: A RARE CASE OF PAPILLARY THYROID CARCINOMA WITH BRAIN METASTASIS WITHOUT LOCAL RECURRENCE

Abstract Introduction Papillary thyroid carcinoma (PTC) is the most common type of thyroid malignancy and generally has an excellent prognosis. However, distant metastases, particularly to the brain, are rare and associated with poor outcomes. This case report presents a 67-year-old female patient with a history of PTC, who developed brain metastasis in the follow-up. Clinical Case A 67-year-old female patient presented four years ago with a rapidly growing 4 cm mass, extending from the right thyroid gland to the right jugular vein. Fine-needle aspiration biopsy (FNAB) of the lesion was reported as suspicious for follicular neoplasm. Ultrasound imaging revealed pathological lymphadenopathy in the bilateral cervical chains. The patient underwent total thyroidectomy and bilateral neck dissection. Pathology confirmed the diagnosis of the follicular variant of papillary thyroid carcinoma (PTC), with extrathyroidal extension into muscle and adipose tissue, as well as lymph node metastases. After surgery, while the patient's TSH level was 68.6 µIU/ml, her thyroglobulin (Tg) level was 1.02 ng/ml, with negative anti-Tg antibodies. The patient received 150 mCi of radioactive iodine (RAI) approximately 3 months after surgery. One year after RAI therapy, a whole-body iodine scan showed no evidence of residual disease or metastasis. Follow-up Tg levels of the patient remained around 0.04 ng/ml. 3.5 years after surgery, there was a slight increase in thyroglobulin levels (0.26 ng/ml), although no recurrent disease was detected on ultrasound of the thyroid bed. The patient was planned to be under close surveillance but she did not come to visits regularly. The patient subsequently presented with complaints of imbalance and brain MRI revealed a mass lesion measuring 46x43x55 mm in the frontal region. The mass was surgically resected, and the pathology confirmed metastatic papillary thyroid carcinoma (PTC). A postoperative whole-body iodine scan was negative for metastatic disease. At that time, the TSH level was 0.5 µIU/ml, and thyroglobulin (Tg) was 0.04 ng/ml. Cranial radiotherapy was planned for the patient. Conclusion Approximately 10% of patients with PTC develop distant metastases, most commonly to the lungs and bones. Brain metastasis from PTC is extremely rare, occurring in less than 1% of cases. Due to its rarity, brain metastasis from PTC is not typically considered in the differential diagnosis for patients presenting with new neurological symptoms, potentially delaying diagnosis. Despite aggressive treatment, the prognosis for patients with PTC and brain metastasis remains poor, with a median survival of approximately 17 months. This case highlights the importance of considering the possibility of brain metastasis in high-risk PTC patients who present with neurological symptoms despite the lack of significant elevation in thyroglobulin (Tg) levels or evidence of recurrence on neck ultrasound.

Regional Gene Therapy for Bone Tissue Engineering: A Current Concepts Review

The management of segmental bone defects presents a complex reconstruction challenge for orthopedic surgeons. Current treatment options are limited by efficacy across the spectrum of injury, morbidity, and cost. Regional gene therapy is a promising tissue engineering strategy for bone repair, as it allows for local implantation of nucleic acids or genetically modified cells to direct specific protein expression. In cell-based gene therapy approaches, a variety of different cell types have been described including mesenchymal stem cells (MSCs) derived from multiple sources—bone marrow, adipose, skeletal muscle, and umbilical cord tissue, among others. MSCs, in particular, have been well studied, as they serve as a source of osteoprogenitor cells in addition to providing a vehicle for transgene delivery. Furthermore, MSCs possess immunomodulatory properties, which may support the development of an allogeneic “off-the-shelf” gene therapy product. Identifying an optimal cell type is paramount to the successful clinical translation of cell-based gene therapy approaches. Here, we review current strategies for the management of segmental bone loss in orthopedic surgery, including bone grafting, bone graft substitutes, and operative techniques. We also highlight regional gene therapy as a tissue engineering strategy for bone repair, with a focus on cell types and cell sources suitable for this application.

C60 fullerene improves the contractile activity of the injured rat muscle gastrocnemius

The powerful antioxidant properties of C60fullerenes have been widely used in biomedical nanotechnology. Owing to the negative effects of free radicals in oxidative stress processes, antioxidants are required to protect injured muscles. Here, the effect of water-soluble C60fullerenes (daily oral dose 1 mg kg-1) on the process of restoration of contractile activity of skeletal muscle of rats (muscle gastrocnemius) 15 d after the initiation of open trauma of different severity was studied for the first time. The structural organization of C60fullerene nanoparticles in aqueous solution was analyzed by dynamic light scattering and atomic force microscopy techniques. Such biomechanical parameters ofmuscle gastrocnemiuscontraction as integrated muscle power, levels of generation of its maximum and minimum force, and time interval until reaching 50% of the level of force response of the muscle were analyzed. Such biochemical indices as concentrations of c-reactive protein, creatinine, and lactate in the rat blood, as well as indices of pro- and antioxidant balance (activities of superoxide dismutase and catalase, the concentration of reduced glutathione) in the blood and muscle tissue of experimental animals, were investigated. It was found that application of water-soluble C60fullerenes statistically significantly improves biomechanical parameters of contraction of injuredmuscle gastrocnemiusat the level of 30-45 ± 3%, which is confirmed by normalization of biochemical indices in the blood and muscle tissue of rats at the level of 35-50 ± 3% and 20-37 ± 3%, correspondingly, relative to the open injury group. These findings open the possibility of using C60fullerenes as potential therapeutic nanoagents capable of correcting pathological states of the muscular system during the physiological repair of open injuries.

Testosterone Modulation of Muscle Transcriptomic Profile During Lifestyle Therapy in Older Men with Obesity and Hypogonadism

ABSTRACTBackgroundTestosterone replacement therapy (TRT) added to lifestyle therapy can mitigate weight‐loss–induced reduction of muscle mass and bone mineral density (BMD) in older men with obesity and hypogonadism.ObjectiveTo investigate the molecular mechanisms underlying the attenuation of muscle and BMD loss in response to TRT during intensive lifestyle intervention in this high‐risk older population.MethodsAmong 83 older (≥ 65 years) men with obesity (BMI ≥ 30 kg/m2) and hypogonadism (early AM testosterone persistently &lt; 300 ng/dL) associated with frailty (Modified Physical Performance Test score ≤ 31) randomized into 26‐week lifestyle therapy plus testosterone (LT+TRT) or placebo (LT+Pbo) in the LITROS trial, 38 underwent serial muscle biopsies for the muscle transcriptomics substudy.ResultsDespite similar ~10% weight loss, lean body mass and thigh muscle volume decreased less in LT+TRT than LT+Pbo (−2% vs. −4%, respectively; p = 0.04). Hip BMD was preserved in LT+TRT compared with LT+Pbo (0.4% vs. −1.3%; p = 0.03). Muscle strength increased similarly in LT+TRT and LT+Pbo (23% vs. 24%; p = 0.95). Total testosterone increased more in LT+TRT than LT+Pbo (133% vs. 32%; p = 0.005). Based on Next Generation Sequencing, of the 39 160 and 39 115 genes detected in LT+TRT and LT+Pbo, respectively, 195 were differentially expressed in LT+TRT and 158 in LT+Pbo. Gene Ontology enrichment analyses revealed that in LT+TRT, just four muscle‐related pathways (muscle organ development, muscle organ morphogenesis, regulation of skeletal muscle contraction, muscle atrophy) were downregulated and one pathway (muscle system process) was upregulated. In contrast, in LT+Pbo, nine muscle‐related pathways (muscle system process, muscle tissue development, muscle organ development, skeletal muscle tissue development, skeletal muscle organ development, skeletal muscle cell differentiation, muscle organ morphogenesis, response to stimuli involved in regulation of muscle adaptation, muscle atrophy) and one pathway related to bone (bone mineralization involved in bone maturation) were downregulated. Muscle system process was upregulated in LT+TRT but downregulated in LT+Pbo. RT‐PCR analyses showed that LT+TRT resulted in a higher expression of MYOD1 (p = 0.02) and WNT4 (p = 0.02), key genes involved in muscle and bone metabolism, respectively, compared with LT+Pbo. We also observed significantly higher mRNA expression of MYBPH (p = 0.006), SCN3B (p = 0.02) and DSC2 (p = 0.01), genes involved in the muscle system process, in response to LT+TRT compared with LT+Pbo.ConclusionThe addition of TRT to lifestyle therapy mitigates the weight‐loss–induced reduction of muscle mass and BMD via countering the weight‐loss–induced downregulation of genes involved in muscle and bone anabolism.

Effectiveness of gua sha with Masanggoubang oil in rats with chronic soft tissue injury.

Chronic soft tissue injury is characterized by sterile inflammation and pain. Gua sha with Masanggoubang oil (GSMO) treatment has been found to possess anti-inflammatory and analgesic effects. To explore the mechanism of GSMO in chronic soft tissue injuries. Fifty male rats were randomly divided into 5 groups (n = 10): 1) control group; 2) chronic soft tissue injury model group; 3) GSMO group; 4) inunction with Masanggoubang oil (IMO) group; and 5) ua sha with tea oil (GSTO) group. The control group and model group received no treatment, while the GSTO group and GSMO group received gua sha therapy with tea oil or Masanggoubang oil on the injured sites. The rats in the IMO group were treated with Masanggoubang oil inunction on the injured sites once every other day, 4 times in total. All animals were sacrificed 48 h after the last treatment. Muscle tissue sections from the injured sites of the rats were stained with hematoxylin & eosin (H&E) staining to observe pathological changes. The protein levels of tumor necrosis factor alpha (TNF-α), interleukin 1β (IL-1β), interleukin 6 (IL-6), inducible nitric oxide synthase (iNOS), and β-endorphin (β-EP) in the rats' skin, serum, and muscle were determined using enzyme-linked immunosorbent assay (ELISA). Gua sha with Masanggoubang oil treatment alleviated necrosis and the denaturation of muscle fibers at the injured sites, reduced connective tissue proliferation and scar tissue generation, downregulated the levels of TNF-α, IL-6 and iNOS in the skin and TNF-α, IL-1β, IL-6, and iNOS in the muscle and serum, and upregulated β-EP levels in the muscle. Gua sha with Masanggoubang oil treatment significantly improved the inflammatory response in rats with chronic soft tissue injury, which may be associated with a reduction of M1 macrophage polarization in the peripheral blood and local tissues. Additionally, the combination of gua sha therapy and Masanggoubang oil may have a synergistic effect in treating chronic soft tissue injuries.

Anthropometry, body composition, somatotype and asymmetry of canoe sprint world champion: A case study.

Background: Body composition is a determinant of physical fitness and sports performance. Aim: To describe the anthropometric characteristics, body composition, somatotype, and asymmetries of the 2023 world champion in the C1-1000 canoeing event. Methods: Dual-energy X-ray absorptiometry (DXA), bioelectrical impedance analysis (BIA), and anthropometry were used to describe the athlete's body composition. Results: The results showed a fat tissue distribution of 16.3% by DXA, 15.9% by BIA, and 19.0% by anthropometry. Muscle tissue was reported at 32.0 kg (47.5%) by BIA and 34.1 kg (50.6%) by anthropometry. Conclusions: The athlete exhibits low levels of fat mass with high lean mass, factors that enable optimal development in world-class sports.

Label-free proteomic analysis of Duchenne and Becker muscular dystrophy showed decreased sarcomere proteins and increased ubiquitination-related proteins

Muscular dystrophies (MD) are a group of hereditary diseases marked by progressive muscle loss, leading to weakness and degeneration of skeletal muscles. These conditions often result from structural defects in the Dystrophin–Glycoprotein Complex (DGC), as seen in Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). Since MDs currently have no cure, research has focused on identifying potential therapeutic targets to improve patients’ quality of life. In this study, skeletal muscle tissue samples from DMD and BMD patients, as well as non-dystrophic controls, were analyzed using label-free mass spectrometry (MS/MS) to characterize the proteomic profile of these conditions and identify biomarkers for differential diagnosis. In-silico analysis revealed that dystrophic muscle tissues are linked to biological processes related to cellular energy metabolism, including oxidation of organic compounds, energy production, and cellular respiration. Enrichment of functions associated with cell structure and RNA binding was also observed, including cytoskeletal protein binding and RNA binding. The human phenotypes most related to the proteomic signature were abnormal circulating metabolites, muscle physiology, and weakness. Quantitative analysis identified significant changes in proteins associated with sarcomere organization and protein ubiquitination, such as myomesin, myozenin, and E3 ubiquitin-protein ligase rififylin, suggesting these as potential therapeutic targets.

GenAI synthesis of histopathological images from Raman imaging for intraoperative tongue squamous cell carcinoma assessment

The presence of a positive deep surgical margin in tongue squamous cell carcinoma (TSCC) significantly elevates the risk of local recurrence. Therefore, a prompt and precise intraoperative assessment of margin status is imperative to ensure thorough tumor resection. In this study, we integrate Raman imaging technology with an artificial intelligence (AI) generative model, proposing an innovative approach for intraoperative margin status diagnosis. This method utilizes Raman imaging to swiftly and non-invasively capture tissue Raman images, which are then transformed into hematoxylin-eosin (H&E)-stained histopathological images using an AI generative model for histopathological diagnosis. The generated H&E-stained images clearly illustrate the tissue’s pathological conditions. Independently reviewed by three pathologists, the overall diagnostic accuracy for distinguishing between tumor tissue and normal muscle tissue reaches 86.7%. Notably, it outperforms current clinical practices, especially in TSCC with positive lymph node metastasis or moderately differentiated grades. This advancement highlights the potential of AI-enhanced Raman imaging to significantly improve intraoperative assessments and surgical margin evaluations, promising a versatile diagnostic tool beyond TSCC.

“Omics” and Postmortem Interval Estimation: A Systematic Review

Postmortem interval (PMI) estimation is a challenge of utmost importance in forensic daily practice. Traditional methods face limitations in accuracy and reliability, particularly for advanced decomposition stages. Recent advances in “omics” sciences, providing a holistic view of postmortem biochemical changes, offer promising avenues for overcoming these challenges. This systematic review aims at investigating the role of mass-spectrometry-based “omics” approaches in PMI estimation to elucidate molecular mechanisms underlying predictable time-dependent biochemical alterations occurring after death. A systematic search was performed, adhering to PRISMA guidelines, through “free-text” protocols in the databases PubMed, SCOPUS and Web of Science. The inclusion criteria were as follows: experimental studies analyzing, as investigated samples, animal or human corpses in toto or in parts and estimating PMI through MS-based untargeted omics approaches, with full texts in the English language. Quality assessment was performed using STROBE and ARRIVE critical appraisal checklists. A total of 1152 papers were screened and 26 included. Seventeen papers adopted a proteomic approach (65.4%), nine focused on metabolomics (34.6%) and two on lipidomics (7.7%). Most papers (57.7%) focused on short PMIs (&lt;7 days), the remaining papers explored medium (7–120 days) (30.77%) and long PMIs (&gt;120 days) (15.4%). Muscle tissue was the most frequently analyzed substrate (34.6% of papers), followed by liver (19.2%), bones (15.4%), cardiac blood and leaking fluids (11.5%), lung, kidney and serum (7.7%), and spleen, vitreous humor and heart (3.8%). Predictable time-dependent degradation patterns of macromolecules in different biological substrates have been discussed, with special attention to molecular insights into postmortem biochemical changes.