Electron Microscopy Of Tissue Sections Research Articles

A device for staining tissue sections for electron microscopy.

A device for staining tissue sections for electron microscopy.

Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium.

Descriptions of three heavy metal stains and methods of application to tissue sections for electron microscopy are presented. Lead hydroxide stains rather selectively two types of particles in liver: those associated with the endoplasmic reticulum and containing ribonucleic acid and other somewhat larger particles. Barium hydroxide emphasizes certain bodies within vesicles of the Golgi region of hepatic cells. Alkalized lead acetate is useful as a general stain, as are also lead and barium hydroxides.

Staining of tissue sections for electron microscopy with heavy metals.

Heavy metals may be incorporated from solution into tissue sections for electron microscopy. The resulting increase in density of the tissue provides greatly enhanced contrast with minimal distortion. Relative densities of various structures are found to depend on the heavy metal ions present and on the conditions of staining. Certain hitherto unobserved details are revealed and some sort of specificity exists, although the factors involved are not yet understood.

The use of carbon films to support tissue sections for electron microscopy.

The use of carbon films to support tissue sections for electron microscopy.

A method for making ultra-thin tissue sections for electron microscopy at high resolution.

A method for making ultra-thin tissue sections for electron microscopy at high resolution.