Cucumber Tissues Research Articles

Potential of fibrinolytic protease enzyme from tissue of sand sea cucumber (Holothuria scabra) as thrombolysis agent

Cardiovascular diseases (CVD) are still the main cause of death in modern society with thrombosis as their most common underlying pathology. The occurrence of thrombosis is characterized by thrombus formation in the circulatory system of the body causing blood clot blockage. Thrombus blocking blood vessels can be destroyed through fibrinolysis by plasmin. Commonly administered fibrinolytic drugs activate plasminogen and convert plasminogen to plasmin which degrades fibrin. However, these agents have limitations such as higher costs, shorter half-lives, allergic reactions and intravenous administration-caused bleeding. Hence, more effective and safer antithrombotic drugs are needed in anti-thrombosis therapy. This paper was intended to assess the ability of Holothuria scabra tissue fibrinolytic proteases as a thrombolytic agent based on literature studies. Summary of recent studies showed that various strong fibrinolytic enzymes could be produced from various animal tissues such as snake and earthworm, yet from sea organism’ tissues are barely reported. In this literature review, the potential of protease enzymes from tissue of H. scabra as thrombolysis agent is summarized and discussed. Among the findings is that H. scabra has the highest protein content among other sea cucumbers in Indonesia, yet the isolation of protease enzymes from H. scabra tissue has not been reported. Hence, there is high possibility in finding novel proteases, which may include serine and metallo-protease known for their thrombolysis activities.

A programmable powerful and ultra-fast water-driven soft actuator inspired by the mutable collagenous tissue of the sea cucumber

Composition/dimensional adjustment of a bulk PNIPAAm-based water-driven soft actuator tuned actuation force/speed in wide ranges at maximum of 2 N and 3 s−1, respectively, demonstrating immediate potentials in soft robotics and biomedical appliance.

Endophytic establishment of the fungal entomopathogen, Beauveria bassiana (Bals.) Vuil., in cucumber plants

BackgroundThe fungus, Beauveria bassiana (Bals.) Vuil., is one of the most important entomopathogenic fungi (EPFs). Recently, its new role was discovered in nature, to be an endophyte in plants. It has been reported as an endophytic fungus in many monocotyledonous and dicotyledonous plants.Main bodyThe study was conducted to evaluate the ability of the fungus, B. bassiana, to colonize and persist in cucumber plants under laboratory conditions and to detect its systemic growth inside the plant tissues in addition to pathogenicity in the plant. The isolate, B195, of the fungus, B. bassiana, was used. Five different inoculation methods were followed: seed dusting, seed immersion, soil drench, seedling drench, and foliar spray. The fungus, B. bassiana, could persist inside different cucumber tissues up to 90 days from inoculation. Soil drench provided the highest recovery rates, while foliar spray gave the lowest rates. Colonization rates reached 94.44 and 73.68% for stem and 68.26 and 37.79% for root, 30 and 90 days post soil drench, respectively, while in foliar spray, it reached 33.51 and 16.45%, after 30 and 90 days post-treatment, for the stem and 9.45 and 0% for the root, respectively. No negative effects were observed in inoculated plants or on fungal pathogenicity.ConclusionResults showed for the first time the ability of the fungus, B. bassiana, isolate B195, to artificially colonize and survive in different parts of cucumber plants under laboratory conditions by different inoculation methods and to grow systemically in plant tissues. This study is considered a preliminary study to the utilization of the fungus, B. bassiana, as an endophyte in cucumber plants to reduce the density of insect pests.

Genome-Wide Identification and Characterization of the TCP Gene Family in Cucumber (Cucumis sativus L.) and Their Transcriptional Responses to Different Treatments.

TCP proteins are plant-specific transcription factors widely implicated in leaf morphogenesis and senescence, flowering, lateral branching, hormone crosstalk, and stress responses. However, the relationship between the transcription pattern of TCPs and organ development in cucumber has not been systematically studied. In this study, we performed a genome-wide identification of putative TCP genes and analyzed their chromosomal location, gene structure, conserved motif, and transcript expression. A total of 27 putative TCP genes were identified and characterized in cucumber. All 27 putative CsTCP genes were classified into class I and class II. Class I comprised 12 CsTCPs and Class II contained 15 CsTCPs. The 27 putative CsTCP genes were randomly distributed in five of seven chromosomes in cucumber. Four putative CsTCP genes were found to contain putative miR319 target sites. Quantitative RT-PCR revealed that 27 putative CsTCP genes exhibited different expression patterns in cucumber tissues and floral organ development. Transcript expression and phenotype analysis showed that the putative CsTCP genes responded to temperature and photoperiod and were induced by gibberellin (GA)and ethylene treatment, which suggested that CsTCP genes may regulate the lateral branching by involving in multiple signal pathways. These results lay the foundation for studying the function of cucumber TCP genes in the future.

Optimization of RNA In Situ Hybridization for mRNA Localization Detection in Mature Tissue of Cucumber Seedlings.

Cucumber (Cucumis sativus L.) is one of the main vegetable crops in China. The physiological cultivation mechanism and gene function characteristics of cucumber are of great significance to the construction of modern agriculture. Due to the low genetic transformation rate of cucumber, only in situ hybridization, which does not involve the progress of gene modified transformation, is convenient to study mRNA localization, so it is more suitable for determination on mRNA localization in the mature tissue of cucumber. At present, the existing in situ hybridization technology system is more suitable for cucumber meristem than for the mature tissue of cucumber seedlings. Therefore, we optimized the traditional plant in situ hybridization protocol. Taking a known gene CsNPF7.2 (Nitrate Transporter Families protein) as an example, we then optimized the steps of plant tissue culture, gene probe preparation, plant material sampling and fixation, preparation of cross section, hybridization pretreatment, hybridization incubation, chromogenic reaction, microscopy examination, and treatment after reaction termination in order to obtain a new RNA in situ hybridization technique suitable for identification on mRNA localization in mature tissues of cucumber seedlings. This optimized technique will ensure the yield of probes, the integrity of RNA molecules, and the clarity and integrity of plant tissue structure, which is conducive to the study of gene function and screening of key genes in cucumber.

Colonization of human opportunistic Fusarium oxysporum (HOFo) isolates in tomato and cucumber tissues assessed by a specific molecular marker.

Fusarium oxysporum is a large complex cosmopolitan species composed of plant pathogens, human opportunistic pathogens, and nonpathogenic isolates. Many plant pathogenic strains are known based on host plant specificity and the large number of plant species attacked. F. oxysporum is an opportunistic pathogen in humans with a compromised immune system. The objectives of this study were: (1) to develop a specific marker to detect human opportunistic F. oxysporum (HOFo) isolates; (2) to determine whether or not HOFo isolates can colonize and cause disease symptoms in plants; and (3) to assess Taiwan isolates sensitivity to two agro-fungicides. The primer pair, Primer 5/ST33-R, specifically amplifying Taiwan and international reference HOFo isolates was developed and used to detect and assess the distribution of a Taiwan isolate in inoculated tomato plants and tomato and cucumber fruit. Taiwan HOFo isolate MCC2074 was shown to colonize tomato roots, hypocotyls, and cotyledons, but did not show any visible symptoms. Four days after surface inoculation of tomato and cucumber fruit with the same isolate, MCC2074 was detected in the pericarp and locular cavities of both tomato and cucumber fruit and in columella of tomato fruit. Three Taiwan HOFo isolates were found to be moderately sensitive to azoxystrobin and highly sensitive to difenconazole.

Effects of pulsed magnetic field on freezing kinetics and physical properties of water and cucumber tissue fluid

In this paper, a special experimental device was designed to study the effect of pulsed magnetic fields (PMF) on freezing kinetics and physical properties of water and cucumber tissue fluid. Pure water and cucumber tissue fluid samples with 3.5 mL were frozen in PMF generated by a pair of Helmholtz coils. The magnetic flux density ranged from 0 to 6 mT in the experiment. The results show that PMF treatment significantly reduced the freezing point temperature (Tice) of water and cucumber tissue fluid with 0.59 °C and 0.74 °C, respectively. Within a certain range of magnetic flux density, PMF treatment not only significantly reduced initial nucleation temperature and the temperature of end point of freezing, but also significantly reduced the phase transition time and the tempering time of both water and cucumber tissue fluid. However, the effects of PMF treatment on the total freezing time of water and cucumber tissue fluid were different. These may be related to the effects of Lorentz forces generated by PMF on the hydrogen bonds, water molecule clusters, ice crystals, charged particles and ferromagnetic particles in the fluid.

Genome wide identification, characterization and expression analysis of HD-ZIP gene family in Cucumis sativus L. under biotic and various abiotic stresses

Information retrieved from genomic assembly may provide important clues and various molecular aspects in plants. Our research identified 40 CsHDZ genes in the Cucumber genome database. Subsequently; we performed the conserved motif and domain analysis of CsHDZ proteins. The phylogeny of the CsHDZ proteins further divides into 4 subfamilies (HD-ZIP I, HD-ZIP II, HD-ZIP III, and HD-ZIP IV) based on the structural similarities and functional diversities. The GO (Gene ontology) analysis of CsHDZ proteins showed that they are responsive to environmental stimuli and involved in numerous growth and developmental processes. The qRT-PCR analysis of 11 CsHDZ genes showed that they are expressed in all the tested tissues of cucumber. The differential expression pattern of CsHDZ genes unfolded their possible involvement in responding to various abiotic stresses and powdery mildew stress. It has been found that the CsHDZ22 localized in the nucleus which possibly participates in the regulatory mechanisms of various biological and cellular processes. In the light of above-mentioned outcomes, it has been deducted that CsHDZ genes in the Cucumis sativus genome play an important role in mediating the resistance to various abiotic stresses and powdery mildew stress as well as provide significant clues for functional studies.

Behavioural response of different epithelial tissues of sea cucumber (Apostichopus japonicus) to light and differential expression of the light-related gene Pax6

ABSTRACT In this study, we evaluated the behavioural characteristics of the epithelial tissues of sea cucumber (Apostichopus japonicus) subjected to illumination. The behavioural results indicated that the tentacles, tube feet, and spines were sensitive to light with structural changes. Among the three epithelial tissues, the tentacles were the most sensitive with the shortest reaction and recovery times, whereas the tube feet and spines had longer reaction times. Quantitative real-time PCR (q-PCR) was used to analyse the changes of Pax6 expressions before and after illumination in the tentacles, tube feet and spines. Q-PCR results indicated that Pax6 expressions in the three above tissues after illumination were higher than that before illumination. We hypothesised that sea cucumber may not have independent photoreceptors, but the epithelial tissues were capable of exhibiting a behavioural response to light. This will allow us to re-examine the functions of all epithelial tissues of sea cucumbers.

Genome-wide analysis of a putative lipid transfer protein LTP_2 gene family reveals CsLTP_2 genes involved in response of cucumber against root-knot nematode (Meloidogyne incognita).

Plant lipid transfer proteins (LTPs) are small basic proteins that play important roles in the regulation of various plant biological processes as well as the response to biotic and abiotic stresses. However, knowledge is limited on how this family of proteins is regulated in response to nematode infection in cucumber. In the present study, a total of 39 CsLTP_2 genes were identified by querying databases for cucumber-specific LTP_2 using a Hidden Markov Model approach and manual curation. The family has a five-cysteine motif (5CM) with the basic form CC-Xn-CXC-Xn-C, which differentiates it from typical nsLTPs. The members of CsLTP_2 were grouped into six families according to their structure and their phylogenetic relationships. Expression data of CsLTP_2 genes in 10 cucumber tissues indicated that they were tissue-specific genes. Two genes showed significant expression change in roots of resistant and susceptible lines during nematode infection, indicating their involvement in response to Meloidogyne incognita. This systematic analysis provides a foundation of knowledge for future studies of the biological roles of CsLTP_2 genes in cucumber in response to nematode infection and may help in the efforts to improve M. incognita-resistance breeding in cucumber.

Comprehensive genomic analysis and expression profiling of the BTB and TAZ (BT) genes in cucumber (Cucumis sativus L.)

BTB-TAZ (BT) proteins are plant-specific transcription factors containing a BTB domain and a TAZ domain. They play vital roles in various biological processes and stress responses. In this study, a total of three BT genes (CsBT1–3) were identified from cucumber genome, and they were unevenly distributed in two of the seven chromosomes. Phylogenetic analysis of the BT proteins from cucumber, Arabidopsis, apple, tomato, and rice revealed that these proteins could be distinctly divided into two groups in accordance with their motif distributions. We also determined the structures of BT genes from cucumber, Arabidopsis, and rice to demonstrate their differences. The quantitative real-time PCR (qRT-PCR) results showed that the CsBT genes displayed differential expression patterns in cucumber tissues, and their expression was regulated by cold, salt, and drought stresses. These findings suggest that CsBT genes may participate in cucumber development and responses to various abiotic stresses.

Magnetic Elastomers with Smart Variable Elasticity Mimetic to Sea Cucumber.

A magnetic-responsive elastomer consisting of magnetic elastomer and zinc oxide with a tetrapod shape and long arms was fabricated mimetic to the tissue of sea cucumber in which collagen fibrils are dispersed. Only the part of magnetic elastomer is active to magnetic fields, zinc oxide plays a role of reinforcement for the chain structure of magnetic particles formed under magnetic fields. The magnetic response of storage modulus for bimodal magnetic elastomers was measured when the magnetic particle was substituted to a nonmagnetic one, while keeping the total volume fraction of both particles. The change in storage modulus obeyed basically a mixing rule. However, a remarkable enhancement was observed at around the substitution ratio of 0.20. In addition, the bimodal magnetic elastomers with tetrapods exhibited apparent change in storage modulus even at regions with a high substitution ratio where monomodal magnetic elastomers consist of only magnetic particles with less response to the magnetic field. This strongly indicates that discontinuous chains of small amounts of magnetic particles were bridged by the nonmagnetic tetrapods. On the contrary, the change in storage modulus for bimodal magnetic elastomers with zinc oxide with irregular shape showed a mixing rule with a substitution ratio below 0.30. However, it decreased significantly at the substitution ratio above it. The structures of bimodal magnetic elastomers with tetrapods and the tissue of sea cucumber with collagen fibrils are discussed.

Expression and functional analysis of the propamocarb-related gene CsMAPEG in cucumber

BackgroundPropamocarb (PM) is one of the main pesticides used for controlling cucumber downy mildew. However, due to its volatility and internal absorption, PM can easily form pesticide residues on cucumber fruits that seriously endanger human health and pollute the environment. The breeding of new cucumber varieties with a low abundance of PM residues via genetic methods constitutes an effective strategy for reducing pesticide residues and improving cucumber safety and quality. To help elucidate the molecular mechanism resulting in a low PM residue abundance in cucumber, we used the cucumber cultivar ‘D0351’ (which has the lowest PM residue content) as the test material and identified genes related to low PM residue abundance through high-throughput tag-sequencing (Tag-Seq).ResultsCsMAPEG was constitutively expressed and showed both varietal and organizational differences. This gene was strongly expressed in ‘D0351’. The expression levels of CsMAPEG in different cucumber tissues under PM stress were as follows: fruit>leaf>stem>root. CsMAPEG can respond to salicylic acid (SA), gibberellin (GA) and Corynespora cassiicola Wei (Cor) stress and thus plays an important regulatory role in plant responses to abiotic and biological stresses. The PM residue abundance in the fruits of CsMAPEG-overexpressing plants was lower than those found in antisense CsMAPEG plants and wild-type plants at all tested time points. The results revealed that CsMAPEG played a positive role in reducing the PM residue abundance. A CsMAPEG sense construct increased the contents of SOD, POD and GST in cucumber fruits, enhanced the degradation and metabolism of PM in cucumber, and thus effectively reduced the pesticide residue abundance in cucumber fruits.ConclusionsThe expression patterns of CsMAPEG in cucumber cultivars with high and low pesticide residue abundances and a transgenic verification analysis showed that CsMAPEG can actively respond to PM stress and effectively reduce the PM residue abundance in cucumber fruits. The results of this study will help researchers further elucidate the mechanism responsible for a low PM residue abundance in cucumber and lay a foundation for the breeding of new agricultural cucumber varieties with low pesticide residue abundances.

Copper ferrite nanoparticles as nutritive supplement for cucumber plants grown under hydroponic system

For high plant growth and efficient production, manufacture of new nano-fertilizers is an important strategy to repair the nutrients shortage. Copper ferrite nanoparticles (CuFe2O4 NPs) were manufactured via co-precipitation method with the aim to understand the role of different concentration of them (0.0, 0.04, 0.2, 1, and 5 ppm) on growth, chlorophyll content and antioxidant enzymes activities of cucumber plants grown in hydroponic system. The prepared sample was studied by various mechanisms such as: X-ray diffraction (XRD), Infrared spectroscopy and vibrating sample magnetometer. XRD pattern implies the nanocrystalline nature of the synthesized sample, where the crystallite size is 30.7 nm. Exposure to CuFe2O4 NPs caused an increase in the fresh weight and protein content of cucumber plants. Also, superoxide dismutase and peroxidase activities of cucumber shoots and roots were significantly increased. The uptake of Fe and Cu by cucumber tissues was significantly enhanced by application of CuFe2O4 NPs.AbbreviationsCCIchlorophyll content indexCuFe2O4 NPscopper ferrite nanoparticlesFWfresh weightICPinductively coupled plasmaIRinfrared spectroscopyO2˙ˉsuperoxide anion;PODperoxidaseROSreactive oxygen speciesSODsuperoxide dismutaseTFtranslocation factorVSMvibrating sample magnetometerXRDX-ray diffraction

Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity

Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Cucumber plants were subjected to moderate Cu toxicity stress (10 μM Cu) without (−Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 μM Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.

Transcriptome Profile Analysis Reveals that CsTCP14 Induces Susceptibility to Foliage Diseases in Cucumber.

Foliage diseases are prevalent in cucumber production and cause serious yield reduction across the world. Identifying resistance or susceptible genes under foliage-disease stress is essential for breeding resistant varieties, of which leaf-specific expressed susceptible genes are extremely important but rarely studied in crops. This study performed an in-depth mining of public transcriptome data both in different cucumber tissues and under downy mildew (DM) inoculation, and found that the expression of leaf-specific expressed transcription factor CsTCP14 was significantly increased after treatment with DM, as well as being upregulated under stress from another foliage disease, watermelon mosaic virus (WMV), in susceptible cucumbers. Furthermore, the Pearson correlation analysis identified genome-wide co-expressed defense genes with CsTCP14. A potential target CsNBS-LRR gene, Csa6M344280.1, was obtained as obviously reduced and was negatively correlated with the expression of the susceptible gene CsTCP14. Moreover, the interaction experiments of electrophoretic mobility shift assay (EMSA) and yeast one-hybrid assay (Y1H) were successfully executed to prove that CsTCP14 could transcriptionally repress the expression of the CsNBS-LRR gene, Csa6M344280.1, which resulted in inducing susceptibility to foliage diseases in cucumber. As such, we constructed a draft model showing that the leaf-specific expressed gene CsTCP14 was negatively regulating the defense gene Csa6M344280.1 to induce susceptibility to foliage diseases in cucumber. Therefore, this study explored key susceptible genes in response to foliage diseases based on a comprehensive analysis of public transcriptome data and provided an opportunity to breed new varieties that can resist foliage diseases in cucumber, as well as in other crops.

Cloning and Functional Characterization of CsUGD2 in Cucumber (Cucumis sativus L.)

UGD gene encodes UDP-glucose dehydrogenase (UGD) which is a key enzyme in the biosynthesis of cell wall, and it catalyses the irreversible oxidation of UDP-glucose (UDP-Glc) into UDP-glucuronic acid (UDP-GlcA). In cucumber, the expression level of CsUGD2 genes was higher in phloem tissues of pedicel and fruit than that in stalk. This study investigated the function of CsUGD2 in cucumber by different methods. Structure analysis indicated that CsUGD2 gene only has an exon with a length of 1,443 bp. Protein alignment suggested that UGD protein was highly conservative in different species. Phylogenetic analysis showed that CsUGD2 protein and CmoUGD2 protein form a same clade which is far away from UGDs in Arabidopsis. Real-time fluorescence quantitative analysis of CsUGD2 in different tissues of cucumber in the same period showed that CsUGD2 expressed highest in the root of cucumber. When we transformed CsUGD2 into wild type Arabidopsis, the roots of transgenic plants were shorter and the flowering time was delayed. These results suggested that CsUGD2 may play an important role in the development of cucumber roots and only act on the development of cucumber fruit when compared with other plants fruits. However, the role of CsUGD2 in regulating the growth and development process of cucumber need to further study.

Prediction models for evaluating the uptake of heavy metals by cucumbers (Cucumis sativus L.) grown in agricultural soils amended with sewage sludge.

Heavy metal (HM) concentrations in edible plants can develop many serious health risks to humans. The precise prediction of plant uptake of HMs is highly important. Thus, the present investigation was carried out to develop regression models for predicting the concentrations of HMs in cucumbers (Cucumis sativus L.) from their concentration in the soil and using the organic matter (OM) content and soil pH as co-factors. The results showed that cucumber roots had the highest significant concentrations of all HMs at P< 0.001, except Cd, Cu, and Zn were in fruits. The lowest concentrations of Cd, Co, Cr, Mn, Ni, and Pb were recorded in stems. HM concentrations in cucumbers were strongly correlated with soil HM, pH, and OM content. Soil pH and OM content had negative and positive correlations with all HMs in cucumber tissues, respectively. Regression analysis indicated that soil HM, pH, and OM contents were good predictors for HM concentrations in cucumbers. The regression models for root Co, Cr, Fe, and Zn were described by high model efficiency values that explain 48-58% variability. The best regression models for cucumber stem were for Cu, Mn, Ni, and Zn that are characterized by high R2 and model efficiency values. For cucumber fruits, R2 values were ranged from 54 to 82%, with best models for Cr, Pb, Cd, Cu, Ni, and Co in the fruit. We expect that these models will be beneficial for risk assessment studies on sewage sludge utilization in agriculture.

Development of a loop-mediated isothermal amplification assay for sensitive and specific detection of Fusarium oxysporum f. sp. cucumerinum Owen

Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum Owen (FOC), is a destructive disease affecting cucumber production worldwide. Developing an accurate and reliable method for detection of FOC is important for disease prediction and control. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed and validated for specific and sensitive detection of FOC. Four LAMP primers were designed based on the sequence of the FOC-specific random amplified polymorphic DNA (RAPD) marker OPZ-12865. LAMP reactions were performed at different temperatures and for different durations, and the optimal temperature and duration were 63 °C for 60 min, respectively. Hence, a LAMP assay for detection of FOC was established. The specificity of the LAMP method was evaluated against 119 isolates of FOC and other pathogens, and only FOC isolates yielded positive results. In sensitivity tests, the lowest concentration of genomic DNA required for the LAMP assay was 10 fg in a 25 μL reaction. The LAMP assay was successfully applied to detect FOC in cucumber tissues and soil from infested fields, and the positive ratios of LAMP, PCR, and traditional tissue isolation for detecting FOC from diseased cucumber root samples were100%, 86.6 and 83.3%, respectively. Therefore, the LAMP assay developed herein should serve as a simple, cost-effective, rapid, highly specific, and sensitive tool for the visual detection of FOC and contribute to improved disease management.

Biocontrol of angular leaf spot disease and colonization of cucumber (Cucumis sativus L.) by endophytic bacteria

Endophytic bacteria (EB) isolated from healthy cucumber plant tissues (e.g., root, stem, leaves) were evaluated as possible biological control of Pseudomonas syringae pv. lachrymans, the causative agent of angular leaf spot disease in cucumber. In this study, 24 endophytic bacteria were selected for tests based on biocontrol traits such as indole-3-acetic acid and siderophore production, solubilization of phosphate, and inhibition growth of P. syringae pv. lachrymans in vitro. Some of the selected endophytes successfully inhibited the pathogen were tested in pots. In the pot experiment, two isolates (CB361-80) and (CC372-83) were selected as the most promising biocontrol agents tested against the pathogen. According to the identification carried out by using 16S rRNA primers and sequence analysis, the two selected endophyte isolates (CB361-80) and (CC372-83) showed 99% similarity to Ochrobactrum pseudintermedium and Pantoea agglomerans, respectively. Two EB isolates colonized seeds at the rate of 3 × 106–7.1 × 107 CFU/g 1 day after seed bacterization. The population of the two EB isolates in cucumber tissues were found to be about 2 × 104–7.5 × 104 CFU/g in roots and 3.8 × 104–2.2 × 105 CFU/g in shoots 45 days after seed bacterization. The populations of the two isolates in the root and shoot tissues did not decrease following the inoculation of the pathogenic bacterium. The endophytic strains CB361-80 (Ochrobactrum sp.) and CC372-83 (Pantoea sp.) successfully colonized the cucumber tissues.