Tissues Of Male Wistar Rats Research Articles

Identification and distribution of m-tyramine in the rat.

A procedure for the quantitative evaluation of m-tyramine in mammalian tissues is described. It involves isolation of the amine by ion-exchange chromatography, followed by conversion to the dansyl derivative, chromatographic separation, and quantitation by the mass spectrometric integrated ion current technique using an isotopically labelled internal standard. The concentrations of m-tyramine in some tissues of male Wistar rats were (mean plus or minus S.D., nanograms per gram): brain 0.32 plus or minus 0.03, heart 0.44 plus or minus 0.13, kidney 12.6 plus or minus 3.4, liver 0.27 plus or minus 0.04, lung 0.33 plus or minus 0.11, spleen 0.25 plus or minus 0.07, and blood 0.15 plus or minus 0.04.

Identification and distribution of p-tyramine in the rat.

A procedure for the quantitative evaluation of p-tyramine in mammalian tissues is described. It involves isolation of the amine by ion-exchange chromatography, followed by conversion to the dansyl derivative, chromatographic separation, and quantitation by the mass spectrometric integrated ion current technique using an isotopically labelled internal standard.The concentrations of p-tyramine in some tissues of male Wistar rats were (mean ± standard deviation (ng/g)): brain 2.0 ± 0.9, heart 3.4 ± 1.4, kidney 32.7 ± 13.1, liver 1.5 ± 0.5, lung 3.0 ± 1.2, and spleen 3.4 ± 1.2. In the brain, hypothalamus contained 11.3 ± 3.7, cerebellum 2.3 ± 1.7, stem 2.2 ± 1.0, caudate nucleus 19.2 ± 2.5, and the 'rest' 1.6 ± 0.4 ng/g, respectively.

Identification and distribution of beta-phenylethylamine in the rat.

A procedure for the quantitative evaluation of some amines present in mammalian tissues has been developed. It includes isolation of the amines by ion exchange chromatography followed by conversion to dansyl derivatives, chromatographic separation, and quantitation by the mass spectrometric integrated ion current technique. The use of an isotopically labelled internal standard improves the precision and sensitivity of the analysis.The concentrations of β-phenylethylamine in some tissues of male Wistar rats were (ng/g); brain 1.8 ± 0.4, heart 5.7 ± 3.1, kidney 20.5 ± 2.2, liver 2.0 ± 0.7, lung 4.0 ± 1.4, and spleen 4.7 ± 2.7. In the brain the hypothalamus contained 25.3 ± 5.0, the cerebellum 3.4 ± 0.5, the stem 2.2 ± 0.9, the caudate nucleus 8.0 ± 0.3, and the 'rest' 1.1 ± 0.2 ng/g, respectively.

Inhibition of steroidogenesis by cyanoketone.

The effect of cyanoketone on some steps of steroidogenesis has been studied on testicular and adrenal tissue of male Wistar rats. Pregnenolone, dehydroepiandrosterone and progesterone (4-C<sup>14</sup>) have been used as substrates. The metabolites have been separated by thin layer chromatography and identified by crystallization to constant specific activity. The results have shown that cyanoketone inhibits the activity of 3Β-hydroxysteroid dehydrogenase; 17Α-hydroxylase is not inhibited in adrenal tissue, while in testicular tissue the metabolic pathway pregnenolone →17Α-hydroxypregnenolone – > dehydroepiandrosterone is altered and testosterone biosynthesis is almost completely inhibited.