Time Research Articles

Human coronavirus 229E inactivation on porous and nonporous materials using dielectric barrier discharge (DBD) plasma.

Viruses can spread through contaminated aerosols and contaminated surface materials, and effective disinfection techniques are essential for virus inactivation. Nonthermal plasma-generated reactive oxygen and nitrogen species can effectively inactivate the coronavirus. We aim to interpret the coronavirus inactivation level and mechanism of surface interaction with materials with and without dielectric barrier discharge (DBD) plasma treatment. Nonthermal plasma, particularly surface-type DBD plasma, can inactivate human coronavirus 229E (HCoV-229E) on porous (paper, wood, mask) and nonporous (plastic, stainless steel, glass, Cu) materials. Virus inactivation was analyzed using a 50% tissue culture infectivity dose (TCID50) using cell line, flow cytometry, and immunofluorescence. Surfaces contaminated with HCoV-229E were treated at different time intervals (0-5 h) with and without plasma exposure (natural decay in ambient air conditions). HCoV-229E persistence conformed to the following order: plastic > cover glass > stainless steel > mask > wood > paper > Cu with and without plasma exposure. HCoV-229E was more stable in plastic, cover glass, and stainless steel in 5 h, and the viable virus titer gradually decreased from its initial log10 order of 6.892 to 1.72, 1.53, and 1.32 TCID50/mL, respectively, under plasma exposure. No virus was observed in Cu after treatment for 5 h. The use of airflow, ambient nitrogen, and argon did not promote virus inactivation. Flow cytometry and immunofluorescence analysis demonstrated a low expression level of spike protein (fluorescence intensity) during plasma treatment and in E and M genes expression compared with the virus control.

Noninvasive Focused Ultrasound-Mediated Delivery of rAAV9-EGFP Vectors for Neuronal Targeting in Rats

Objective: To evaluate the synergistic potential of Focused Ultrasound (FUS) in conjunction with microbubbles (MB) and recombinant adeno-associated virus serotype 9 (rAAV9) vectors for targeted gene delivery to neuronal cells in rats, optimizing gene expression conditions and assessing any adverse effects.Methods: The parameters for permeability enhancement of the rat's blood-brain barrier (BBB) were established using FUS+MB, with MRI scans and Evans Blue (EB) dye assisting in the evaluation. Rats underwent FUS-mediated transfection using rAAV9-Syn-EGFP vectors produced via a triple-transfection in HEK293T cells. Following this, the uptake and expression of GFP in targeted brain regions were evaluated using confocal fluorescence microscopy at various time intervals. Inflammatory responses post-FUS treatment were tracked by observing levels of GFAP, a marker for astrocytic activation, and TNF-α, a pro-inflammatory cytokine. Motor behavior effects post-intervention were gauged using the Rotarod test across multiple groups over a span of four weeks.Results: FUS+MB affected BBB permeability, with optimal results at 4 W for 200 seconds showing 85% permeability and evident Gd-DTPA leakage. Settings beyond these resulted in tissue damage. Control groups exhibited a basal GFP expression of 2% ± 0.5%, whereas FUS+MB with rAAV-EGFP injections substantially increased GFP expression to about 67% ± 6% in targeted neurons. This GFP expression peaked at three weeks post-treatment and remained evident six months later. Following FUS treatment, both GFAP and TNF-α levels underwent fluctuations before eventually nearing their baseline values. The Rotarod test revealed no significant behavioral differences post-treatments among the groups.Conclusions: Combining FUS+MB with rAAV offers an innovative approach to enhance therapeutic delivery to the central nervous system (CNS) by transiently adjusting BBB permeability.

Late Cretaceous-Early Palaeocene lepisosteiform and siluriform fish remains from Central India: palaeoecological, palaeoenvironmental and palaeobiogeographical implications

Late Cretaceous-Early Palaeocene geo-climatic events played an important role in the diversification of the modern ichthyofauna. Lepisosteiformes and Siluriformes are two diverse clades of freshwater fishes, poorly known from India in this time interval. Their fossil record documents their early diversification and can be used to reconstruct palaeobiogeographic relationships among the continental masses during the Late Mesozoic. Indeed, the Cretaceous-Palaeocene is an exceptional time span for the Indian plate as it underwent a primary spatial reorganisation and remarkable geologic and climatic changes with extensive outpouring of the Deccan basaltic magma. Field investigation in a Deccan sedimentary sequence at Kisalpuri, Central India has yielded new, rich, and taxonomically important fossil material of Lepisosteiformes (Lepisosteus indicus, Lepisosteidae) and Siluriformes (Siluriformes indet.), which significantly improves their Cretaceous-Palaeocene fossil record from the Indian subcontinent. These fish lived in freshwater environments such as large rivers, lakes, and coastal areas. Their presence along with other aquatic biotic elements suggests that the Kisalpuri was an abundant aquatic ecosystem rich in nutrients, which attracted a diverse range of organisms to live together with fish. Biostratigraphically, these fishes first emerged in the Late Cretaceous-Early Palaeocene on the Indian subcontinent, and later, they spread to other parts of subcontinent throughout the Cenozoic. Their earliest intra-continental distribution was controlled by the palaeodrainage network influenced by the volcanic flows of the Deccan traps. Their inter-continental palaeobiogeographic distribution was broadly controlled by the sequential break-up of Pangaea. There appear to be close palaeobiogeographic linkages between South America and India during the Cretaceous-Palaeocene era based on the similarity between the Lepisosteiformes and Siluriformes remnants from India and those reported from the Upper Cretaceous of the Bauru Group, Brazil.

Performance analysis of two-way multi-users cooperative communication system based on GSPIM-DCSK scheme

Based on the Joint Grouping Subcarrier-Permutation Index Modulation-DCSK (GSPIM-DCSK) system, we provide a new two-way multi-user half-duplex cooperative communication system in this work. Given its many benefits, the GSPIM-DCSK modulation is specifically selected as a choice for network coding techniques. A permutation index is used in the GSPIM-DCSK system to incorporate additional data bits with the modulating and carrier index bits. Establishing a cooperative multiuser system where users attempt to make contact with one another over a common relay is the main goal of this effort. Multi-user nodes and a single relay with a cooperative operator on staff for decoding and forwarding make up this system. To avoid interference from other users, the user’s nodes multiplex across various time intervals. We derive analytical expressions for the average bit error rate (BER) of the GSPIM-DCSK multi-user cooperative communication system in the presence of additive white Gaussian noise (AWGN) and multiple paths Rayleigh fading channels. The analytical and simulated average BER findings have been contrasted to verify the accurateness of the average BER analysis. Additionally, a comparison is presented between the average bit error rate (BER) performance of a novel GSPIM-DCSK cooperative system and an established DCSK cooperative system. The throughput and link spectral efficiency of this novel cooperative system are analyzed and compared with those of existing traditional cooperative systems. For the two-way two-user cooperative system, this comparison is done with two-way two-user systems like DCSK-CC and one-way cooperative system based on CIM-SR-DCSK-CC; for the two-way three-user cooperative system, it is done with scheme1 and scheme2. To compare and assess the performance of various systems, simulation results are employed. Furthermore, an analysis demonstrates how the proposed cooperative system, when combined with GSPIM-DCSK techniques, improves the efficiency of conventional cooperative DCSK systems.

Mechanism of radial chain rockbursts in a deep granite TBM tunnel under the influence of weak band

Radial chain rockbursts (RCRs) in deep-buried TBM tunnels have prolonged durations and can pose significant safety risks. Further research is needed to deepen our understanding of the mechanisms of recurrence of rockbursts under the influence of structure plane. In this study, the geological characteristics, occurrence characteristics, microseismic (MS) activity laws, and rock mass fracture mechanism of several RCRs influenced by weak band were summarized to reveal the mechanism of recurrence of RCRs. Research shows that under the influence of weak band, (1) RCRs tends to occur in the area where the dip angle is large, and the strike intersects with the tunnel direction at a large angle and forms a small angle with the maximum principal stress. The width of the weak band typically ranges from 0.01 to 1 m, which is positively correlated with the final depth of rockburst pit. The strength of the weak band is relatively “soft” compared to the surrounding bedrock. (2) The depth of each rockburst pit in RCRs gradually decreases, the time interval between adjacent rockbursts gradually increases, and the maximum thickness of rock block in each rockburst gradually increases. (3) The maximum MS energy of MS events and the maximum apparent stress gradually decreased in the development process of RCRs. The region of stress concentration gradually extended to deeper zone, and the distance difference between stress concentration region and sidewall during each rockburst occurrence stage gradually decreased. (4) The effect of weak band on RCRs attributes to the excavation induced local stress concentration near the weak band, and the interface between the weak band and surrounding rock controls the boundary of the rockburst body. The “chain effect” of RCRs mainly shows that the promotion effect of the previous rockburst on the subsequent rockburst, by promoting the stress and rock fracture transferring to the deep zone, and the proportion of shear fractures increases.

Basin entropy as an indicator of a bifurcation in a time-delayed system.

The basin entropy is a measure that quantifies, in a system that has two or more attractors, the predictability of a final state, as a function of the initial conditions. While the basin entropy has been demonstrated on a variety of multistable dynamical systems, to the best of our knowledge, it has not yet been tested in systems with a time delay, whose phase space is infinite dimensional because the initial conditions are functions defined in a time interval [-τ,0], where τ is the delay time. Here, we consider a simple time-delayed system consisting of a bistable system with a linear delayed feedback term. We show that the basin entropy captures relevant properties of the basins of attraction of the two coexisting attractors. Moreover, we show that the basin entropy can give an indication of the proximity of a Hopf bifurcation, but fails to capture the proximity of a pitchfork bifurcation. The Hopf bifurcation is detected because before the fixed points become unstable, a oscillatory, limit-cycle behavior appears that coexists with the fixed points. The new limit cycle modifies the structure of the basins of attraction, and this change is captured by basin entropy that reaches a maximum before the Hopf bifurcation. In contrast, the pitchfork bifurcation is not detected because the basins of attraction do not change as the bifurcation is approached. Our results suggest that the basin entropy can yield useful insights into the long-term predictability of time-delayed systems, which often have coexisting attractors.

Doxorubicin encapsulated blend of sitagliptin-lignin polymeric drug delivery system for effective combination therapy against cancer

In this research, a sitagliptin-lignin biopolymer (SL) containing zinc selenide quantum dots (ZnSe QDs) and doxorubicin (doxo) was synthesized. The fabricated polymeric drug delivery system was characterized via FTIR, XRD, SEM, TGA, IR, and DSC. SLQD-Doxo exhibited an irregular surface with a 32 nm diameter and well-defined surface chemistry. Drug loading efficiency was assessed at different concentrations, pH levels, time intervals, and temperatures, and drug kinetics were calculated. Maximum drug release was observed at 6 μmol concentration after 24 h, pH of 6.5 and 45 °C. The maximum drug encapsulation efficiency was 81.75 %. SLQD-Doxo demonstrated 24.4 ± 1.04 % anti-inflammatory activity, and the maximum lipoxygenase inhibition in a concentration-dependent manner was 71.45 ± 2.02 %, compared to indomethacin, a standard anticancer drug. The designed system was applied to breast cancer MCF-7 cells to evaluate anticancer activity. Cytotoxicity of SLQD-Doxo resulted in 24.48 ± 1.64 dead cells and 74.39 ± 4.12 viable cells. Lignin's polyphenolic nature resulted in good antioxidant activity of LLQD-Doxo. The combination of SLQD-Doxo was appropriate for drug delivery at high temperatures and acidic pH of tumor cells compared to healthy cells.

Downsizing Nanoarchitectonics of Multilayered MXenes electrocatalysts towards real time ion tracking via EQCM and Electrocatalytic Applications

Since their discovery, engineering two-dimensional (2D) MXene materials have attracted rapid interest in both energy storage and conversion applications. Among the several techniques being introduced for enhancing material properties, downsizing is one among the interesting approaches. Downsizing involves the deliberate scaling down of active 2D materials, such as MXenes, systematically. Although major studies are focused on the electrochemical applications of single layered MXene flakes, curiosity in evaluating the downsized multilayered MXenes for electrochemical applications motivates this project. Thus, in the current study, the multilayered bulk MXenes are sequentially stepped down to smaller multilayered fragments at definite time intervals, and subsequently the potential of this procured heterogeneous polydispersed solution are evaluated towards electrochemical applications without any further post-treatments. Real time monitoring of lithium-ion exchange in the downsized MXene materials at various time intervals was tracked using the electrochemical quartz crystal microbalance technique, where downsized MXene system exhibited water-assisted lithium-ion transfer behaviour. Increase in mass exchange was found to increase with increase in downsized MXene systems, thus making it very interesting towards ion storage applications. Further, downsized MXene electrocatalyst material delivered the lowest onset potential for hydrogen production among the set of catalysts studied. In short, this study outlines and pioneers some interesting observations regarding both energy storage and catalytic applications of multilayered downsized MXenes, thereby opening up new possibilities for facile, rapid and cost-effective material fabrication approaches.

Feasibility of self-measurement telemonitoring using a handheld heart sound recorder in patients with heart failure – SELPH multicenter pilot study

BackgroundMulti-parametric assessment, including heart sounds in addition to conventional parameters, may enhance the efficacy of noninvasive telemonitoring for heart failure (HF). We sought to assess the feasibility of self-telemonitoring with multiple devices including a handheld heart sound recorder and its association with clinical events in patients with HF. MethodsAmbulatory HF patients recorded their own heart sounds, mono‑lead electrocardiograms, oxygen saturation, body weight, and vital signs using multiple devices every morning for six months. ResultsIn the 77 patients enrolled (63 ± 13 years old, 84 % male), daily measurements were feasible with a self-measurement rate of >70 % of days in 75 % of patients. Younger age and higher Minnesota Living with Heart Failure Questionnaire scores were independently associated with lower adherence (p = 0.002 and 0.027, respectively). A usability questionnaire showed that 87 % of patients felt self-telemonitoring was helpful, and 96 % could use the devices without routine cohabitant support. Six patients experienced ten HF events of re-hospitalization and/or unplanned hospital visits due to HF. In patients who experienced HF events, a significant increase in heart rate and diastolic blood pressure and a decrease in the time interval from Q wave onset to the second heart sound were observed 7 days before the events compared with those without HF events. ConclusionsSelf-telemonitoring with multiple devices including a handheld heart sound recorder was feasible even in elderly patients with HF. This intervention may confer a sense of relief to patients and enable monitoring of physiological parameters that could be valuable in detecting the deterioration of HF.

Demonstration of Inappropriate Validation Method for a Cracker Baking Process Using Predictive Modeling

Validation of baking processes for the inactivation of Salmonella is complicated by the combined effects of product heating and drying. The goal of this study was to quantitatively evaluate a previously disseminated approach to validating baking processes utilizing a predictive model developed using only isothermal and single-moisture inactivation data for the initially formulated dough. A simple cracker dough was formulated using flour inoculated with a five-strain cocktail of Salmonella. Side-by-side isothermal and baking experiments were performed to estimate Salmonella inactivation kinetics and to quantify survivors in a dynamic environment, respectively. Isothermal, single-moisture inactivation experiments were performed with cracker dough (water activity, aw = 0.956 ± 0.002; moisture content = 0.50 ± 0.01 dry basis) at three temperatures (56, 60, or 63°C) with ≥6 time intervals. Baking experiments were performed in a convection oven at 177°C with samples pulled every 30 s up to 360 s, with an endpoint product aw (25°C) of 0.45. The Salmonella isothermal, single-moisture inactivation kinetics in cracker dough resulted in D60°C and z values of 4.6 min and 4.9°C, respectively; this model was then integrated over the dynamic product temperature profiles from the baking experiments. In the baking experiments, an average of 5-log reductions of Salmonella was achieved by 150 s of treatment; however, >100-log reductions were predicted by the dough-based models at that time point. This fail-dangerous overestimation of Salmonella lethality in crackers explicitly demonstrated that single-level moisture-based prediction models are inappropriate for describing inactivation in a process with both dynamic temperature and moisture, and that model-based validations must incorporate moisture/aw. Furthermore, end-users should exercise caution when utilizing unvalidated models to validate preventive control processes.

Comparison of Greater Occipital Nerve Blockade with Radiofrequency and Steroid in Chronic Migraine

ObjectivesThe study aims to retrospectively compare steroid and radiofrequency treatments for the greater occipital nerve(GON) under ultrasound guidance in chronic migraine. MethodsMonthly average headache attack frequency, attack duration, visual analog scale(VAS) and the number of days analgesics were taken were recorded. Under ultrasound guidance, GON block was administered once a week for a total of four sessions. In the final session, 4mg of dexamethasone was added to the local anesthetic for the steroid group(n:26). Pulsed radiofrequency (RF) treatment was applied to the RF group(n:25) just before the last session of the GON block. The pain course in the week following the procedure monthly average headache attack frequency, attack duration, VAS and the number of days analgesics were taken in a month were compared for both groups at 1-3-6 months. ResultsIn the first month, a statistically significant decrease in attack frequency was observed in the RF group. However, no statistically significant differences were found between the groups in other findings at different time intervals. A significant decrease headache attack duration,VAS, and the number of days analgesics were taken in a month was observed in both treatment groups. Both treatments were found to be effective, but the effect size of the treatment was higher in the RF group compared to the steroid group. ConclusionAlthough the results were better in the group receiving pulsed RF treatment, except for the attack frequency in the first month, no statistically significant superiority of one treatment method over the other was determined.

Assessing the impact of lubricant on liquid-based Pap smear test outcomes: a randomized clinical trial.

Since today cervical cancers are growing, there is an increasing need to use screening and examination methods. Meanwhile, liquid-based Pap smear test is a common screening method for women, which is widely applied today. Studies have found that use of lubricant gel in this test can affect the pathology and cytology results. Accordingly, the authors intended to evaluate the effect of use of lubricant gel on the Pap smear test results. This study was of single-blind clinical trial, the study population consisted of candidate patients for screening in terms of cervical pathology, for whom liquid-based Pap smear was done. In this study, 506 patients participated, divided into two groups of 253. One group used lubricant gel during the Pap smear, while the other group underwent this test without lubricant. The data were analyzed by SPSS 21. The study results indicated that once the two groups were compared in terms of age, interval of menstruation time and intercourse time from the sampling, no significant relationship was found between the two groups (P>0.05). It was also found that use of lubricant did not affect the cytology and pathology results of patients (P>0.05). The use of lubricant gel in patients can reduce pain in patients during examination and testing, but does not affect the cytological and pathological results of patients.

Analysis of drug-related problems in the home medication review practice by the master of pharmacy students

Background: Master’s students are required to perform community service (CS), so a strategy is needed to merge CS into a course and make it a learning method that can be sustainably evaluated. Objective: This study aimed to analyse patients’ problems in using, storing, and disposing of drugs through home medication review (HMR) by master’s students of pharmacy. Subjective self-assessment assesses students’ performance in fulfilling the relevant competencies. Method: This cross-sectional study, conducted for four months, involved 225 patients living near the students’ residences. Semi-structured interviews collected data on subject characteristics and how the patients/family members managed drugs at home. Result: Nine master’s students practised HMR to achieve the learning outcomes of the pharmaceutical care course. The most commonly identified drug-related problems (DRPs) were inappropriate timing or dosing interval (41.2%) and medication nonadherence (25%). Some patients (31.6%) stored drugs without adherence to the provisions, and 70.2% disposed of drugs directly in the trash can. Meanwhile, 88.9% of students strongly agreed that HMR could improve their ability to gather patient data and information. Conclusion: HMR is an excellent patient-oriented practice for master’s students to strengthen and refine their awareness of community needs regarding drug use and management by patients at home.

Trusted Head Node for Node Behaviour Analysis for Malicious Node Detection in Wireless Sensor Networks

There has been a rise in the use of Wireless Sensor Networks (WSNs) in several fields. Depending on the malicious actions, attacks on WSN can take many forms. This research demonstrates how to leverage request and response data from each node to pinpoint the location of malicious nodes. If a node is malevolent, it will prohibit data from being sent to other nodes. They do an excellent job of pretending to be someone else. To enhance route quality, packet dropping can be used to perform a Denial of Service (DoS) assault by rejecting data packets and cutting them off from their destinations. The primary goal of this research is to expose malicious nodes within the network. To avoid being attacked by the false information fed by the adversary through compromised nodes, it is crucial to discover and isolate the nodes that have been compromised. Unfortunately, flat topology networks are difficult to keep safe because of their limited scalability and substantial communication overhead. By computing the packet drop and throughput values with valid nodes, efficient security architecture for the WSN can be proposed, allowing for the detection of malicious activity. If sensor networks are susceptible to attacks, they will be unable to fulfill their duty in a variety of event detection applications. Malicious nodes can cause unacceptable drops in event detection effectiveness and spikes in false alarm rates by producing fake readings and deceptive information. In this research, a method for identifying malicious nodes in WSNs is proposed. This research considers a Trusted Head Node (THN) for monitoring the nodes behaviour in the network to detect the malicious actions in the network. This research proposes a Trusted Head Node for Node Behaviour Analysis for Malicious Node Detection (THN-NBA-MND) in WSN. The proposed model based on node behavior analysis in frequent time intervals; detect the malicious nodes to improve the network performance in data transmission. The proposed model when contrasted with the existing model performs better in malicious node detection.

Helminth community of Nectomys squamipes naturally infected by Schistosoma mansoni in an endemic area in Brazil: a comparison of 22 years apart

The municipality of Sumidouro in the state of Rio de Janeiro, southeastern Brazil, is considered an area with low endemicity of Schistosoma mansoni. In this municipality, the wild water rat Nectomys squamipes is a wild reservoir of S. mansoni. A helminth community survey was carried out on N. squamipes populations in Sumidouro from 1997 to 1999. In the present study, we compared the helminth fauna and the helminth community structure of N. squamipes with a recent survey after a 22-year time interval, considering that the prevalence of S. mansoni infection in humans remained stable and that the area showed the same environmental characteristics. Seventy-three host specimens of N. squamipes collected between 1997 and 1999 and 21 specimens collected in 2021 were analyzed in this study. Seven helminth species were found in each collection period. The nematode Syphacia evaginata was recorded for the first time in N. squamipes in 2021. Syphacia venteli was the most abundant species in both periods and the most prevalent in 2021. During the period from 1997 to 1999, the most prevalent species was Hassalstrongylus epsilon. Significant differences in prevalence and abundance in relation to host sex were observed only for S. mansoni in 1997–1999. Significant differences in the abundance of the helminth species over time were observed only in Physaloptera bispiculata. Hassalstrongylus epsilon, S. venteli and S. mansoni were the dominant species in both periods. Litomosoides chagasfilhoi, Echinostoma paraensei paraensei and P. bispiculata became dominant, codominant and subordinate, respectively, over time. In conclusion, the helminth community of N. squamipes remained stable, with similar species richness, prevalence and abundance values and low beta-diversity over time. The occurrence of S. mansoni in the water rat has remained stable for decades, highlighting its importance for schistosomiasis control.

Development of Shelf Life for Labaneh Using Protective Cultures of L. paracasei and Propionibacterium freudenreichii subsp. shermanii

Labaneh (strained yogurt) is a traditional dairy product. It is usually produced without chemical preservatives as Jordanian standards for this product prevent their addition. Its shelf life is limited to two weeks. This research aims to develop the shelf life of labaneh using eco-friendly methods, via the addition of protective probiotic cultures. Protective probiotic cultures were prepared by activating DVI freeze-dried Propionibacterium freuendereichii, subsp. shermanii and Lactobacillus paracasei in sterilized skim milk. Fifty kg of fresh milk was divided into two portions, the first containing the traditional yogurt starter culture (2%) and the second processed by adding both the starter (2%) and protective probiotic cultures (1%). After fermentation, two types of yogurts were produced, and refrigerated for several hours. They were then salted, strained separately to about 25% total solids in a special cloth to produce labaneh paste. The paste was then distributed into two groups of sterilized jars (21 for each) and placed in refrigerated storage. Both types of labaneh (traditional and developed) were evaluated at different time intervals of refrigerated storage (0, 3, 7, 10, 14, 21, 30 days) for their counts of coliform bacteria, yeast and mould, and lactic acid bacteria (LAB), and for their pH, ash, acidity, moisture, protein, fat and titratable acidity. The probiotic count was also evaluated for the developed labaneh. The shelf life of the developed labaneh was determined based on the acidity, yeast and mould counts and organoleptic characteristics. No obvious and significant undesirable changes were seen at 30 days of refrigeration or went beyond the Jordanian standard for the developed labaneh, compared to the traditional one which started to spoil after 14 days. No coliform bacteria were found after 30 days of refrigerated storage in the traditional and developed labanehs. The counts of yeast and mould were generally higher in the traditional labaneh than in the developed one, while the number of LAB was lower in the traditional labaneh than in the developed one. The pH and moisture of the developed labaneh were generally lower than that of the traditional labaneh, but the titratable acidity, ash, protein and fat were higher in the developed one. Regarding the sensory properties, most of the panelists organoleptically preferred the developed one during the storage period. Therefore, it is concluded that the protective probiotic cultures were effective in extending the shelf life of labaneh.

Effects of Climatic Conditions of Al Seeb in Oman on the Performance of Solar Photovoltaic Panels

Human activities and climatic elements, including temperature, humidity, and wind speed, have an impact on natural dust deposition. Therefore, this study aims to investigate the effects of wind speed, relative humidity, and ambient temperature on the performance of soiled photovoltaic panels in Al Seeb, Oman. The study was conducted by exposing the solar PV panels to outdoor sunlight for a duration of two months. Parameters such as solar radiation, voltage, current, solar panel temperature, wind speed, relative humidity, and ambient temperature were collected in a short time interval. It was observed that the dust densities of 20.7 g/m2, 27 g/m2, and 41.3 g/m2 resulted in electrical power reductions of 18%, 33%, and 40% for the panels uncleaned for one week, two weeks, and three weeks, respectively. The effect of daily dust resulted in an energy reduction of 14%. Moreover, dust deposition decreases when the wind speed increases, resulting in a higher power output and vice versa. The higher the humidity, the stronger the dust’s adhesion to the surface, resulting in more deposition and reduced energy output. The maximum power of output of 82.3 W was achieved at the wind speed of 10 m/s, 34.9% relative humidity, and ambient temperature of 38.5°C. The PV panels' surface temperature is always higher than the surrounding air temperature, necessitating optimal cooling to produce more power.

THE POTENTIAL ROLE OF EXERCISE IN DELAYING GENETIC DESTINY: EXAMINING THE RELATIONSHIP BETWEEN EXERCISE AND KEY BIOMARKERS IN DOMINANTLY INHERITED ALZHEIMER’S DISEASE

INTRODUCTION Greater physical activity is associated with reduced risk for Alzheimer’s disease (AD), and lower levels of AD-related biomarkers, such as beta-amyloid (Aβ) and tau, measured in the cerebrospinal fluid (CSF) and brain. A small proportion of Alzheimer’s disease (<1%) cases are caused by a rare dominant genetic mutation. The aim of the current study was to examine associations between self-reported exercise participation and AD-related biomarkers (from CSF and brain imaging) over time, in individuals we know will develop Alzheimer’s disease at an early age (i.e., dominant AD mutation carriers). METHODS The sample included n = 308 mutation carriers from the Dominantly Inherited Alzheimer’s Network (DIAN) study with data available for self-reported exercise participation, brain imaging (hippocampal volume, total brain volume, gray matter volume, white matter hyperintensities, brain Aβ levels), and biomarkers quantified from CSF (several Aβ and tau species and ratios). Participants were assessed regularly (time interval depending on mutation type) from baseline to 10+ years post-baseline. Associations between exercise and AD biomarkers (i.e., from brain imaging and CSF) were examined using linear mixed models, corrected for various confounding variables. RESULTS The sample had a mean age of 39.7 ± 10.8 years and were 56% female. Greater baseline exercise was associated with a slower decrease in right (B=0.06, p < 0.001) and left (B=0.06, p<0.05) hippocampal volume; and slower accumulation of brain Aβ (B=0.04, p<0.001). CONCLUSION These findings demonstrate that exercise is associated with more favourable profiles of AD-related biomarkers in those with ADAD mutations. This work may have implications for our understanding of how exercise influences disease development in late-onset sporadic AD. Nevertheless, the causal direction of our findings is difficult to ascertain, and future study designs investigating the therapeutic potential of exercise in both ADAD and late-onset AD should be considered.

Mathematical modeling and optimal control strategies to limit the spread of fowl pox in poultry

This paper presents a mathematical model aimed at elucidating the dynamics of fowl pox transmission within poultry populations. The model focuses on implementing control strategies to manage the spread of the disease, considering two primary modes of transmission: direct contact and transmission via mosquitoes. Our objective is to enhance understanding of disease propagation and to propose a control strategy aimed at minimizing the number of infected birds Ib(t) and increasing the number of recovered birds Rb(t) during the time interval t0,tf, while also minimizing the costs associated with implementing the control strategy. The proposed mathematical framework facilitates the integration of various control strategies to effectively manage fowl pox transmission dynamics. By employing Pontryagin’s maximum principle, efficient control measures are identified to mitigate the spread of the disease. Finally, numerical simulations are performed to verify the theoretical analysis using MATLAB.

Every minute matters: Improving outcomes for penetrating trauma through prehospital advanced resuscitative care.

Prehospital resuscitation with blood products is gaining popularity for patients with traumatic hemorrhage. The MEDEVAC trial demonstrated a survival benefit exclusively among patients who received blood or plasma within 15 minutes of air medical evacuation. In fast-paced urban EMS systems with a high incidence of penetrating trauma, mortality data based on the timing to first blood administration is scarce. We hypothesize a survival benefit in patients with severe hemorrhage when blood is administered within the first 15 minutes of EMS patient contact. This was a retrospective analysis of a prospective database of prehospital blood (PHB) administration between 2021 and 2023 in an urban EMS system facing increasing rates of gun violence. PHB patients were compared to trauma registry controls from an era before prehospital blood utilization (2016-2019). Included were patients with penetrating injury and SBP ≤ 90 mmHg at initial EMS evaluation that received at least one unit of blood product after injury. Excluded were isolated head trauma or prehospital cardiac arrest. Time to initiation of blood administration before and after PHB implementation and in-hospital mortality were the primary variables of interest. A total of 143 patients (PHB = 61, controls = 82) were included for analysis. Median age was 34 years with no difference in demographics. Median scene and transport intervals were longer in the PHB cohort, with a 5-minute increase in total prehospital time. Time to administration of first unit of blood was significantly lower in the PHB vs. control group (8 min vs 27 min; p < 0.01). In-hospital mortality was lower in the PHB vs. control group (7% vs 29%; p < 0.01). When controlling for patient age, NISS, tachycardia on EMS evaluation, and total prehospital time interval, multivariate regression revealed an independent increase in mortality by 11% with each minute delay to blood administration following injury (OR 1.11, 95%CI 1.04-1.19). Compared to patients with penetrating trauma and hypotension who first received blood after hospital arrival, resuscitation with blood products was started 19 minutes earlier after initiation of a PHB program despite a 5-minute increase in prehospital time. A survival for early PHB use was demonstrated, with an 11% mortality increase for each minute delay to blood administration. Interventions such as PHB may improve patient outcomes by helping capture opportunities to improve trauma resuscitation closer to the point of injury. Prospective, Level IV.