Burst Time Research Articles

Changes in Environmental Conditions Modify Infection Kinetics of Dairy Phages.

Latent period, burst time, and burst size, kinetic parameters of phage infection characteristic of a given phage/host system, have been measured for a wide variety of lactic acid bacteria. However, most studies to date were conducted in optimal growth conditions of host bacteria and did not consider variations due to changes in external factors. In this work, we determined the effect of temperature, pH, and starvation on kinetic parameters of phages infecting Lactobacillus paracasei, Lactobacillus plantarum, and Leuconostoc mesenteroides. For kinetics assessment, one-step growth curves were carried out in MRS broth at optimal conditions (control), lower temperature, pH 6.0 and 5.0 (MRS6 and MRS5, respectively), or in medium lacking carbon (MRSN) or nitrogen (MRSC) sources. Phage infection was progressively impaired as environmental conditions were modified from optimal. At lower temperature or pH, infection was delayed, as perceived by longer latent and burst times. Burst size, however, was lower, equal or higher than for controls, but this effect was highly dependent on the particular phage-host system studied. Phage infection was strongly inhibited in MRSC, but only mildly impaired in MRSN. Nevertheless, growth of all the bacterial strains tested was severely compromised by starvation, without significant differences between MRSC and MRSN, indicating that nitrogen compounds are specifically required for a successful phage infection, beyond their influence on bacterial growth.

Effects of chin-up posture on the sequence of swallowing events.

Chin-up posture is frequently used to manage oral dysphagia after head and neck cancer. This prospective study investigates the effects of chin-ups on the sequence of pharyngeal swallowing events. Twelve healthy young adults performed 45 consecutive swallows of 5 mL water across 3 phases on videofluoroscopy: 5 swallows in the neutral head position; 30 swallows during chin-up posture; and 10 swallows in the neutral head position. Swallowing kinematic and bolus flow measures for 9 swallowing events were recorded. Linear trends were analyzed across 30 chin-up swallows; pairwise comparison was used to compare the 3 phases. Time to hyoid peak and laryngeal vestibule closure changed abruptly during chin-up swallowing compared to the initial neutral position. No measure changed across 30 chin-up swallows. Time of hyoid burst decreased upon returning to the neutral position. Our findings indicate that chin-up posture challenges the pharyngeal sequence of events for both swallowing kinematics and bolus flow. © 2017 Wiley Periodicals, Inc. Head Neck 39: 947-959, 2017.

Population variation in drought resistance and its relationship with adaptiveand physiological seedling traits in Turkish red pine (Pinus brutia Ten.)

Variation in drought resistance and its relationship with adaptive and physiological traits in forest trees are important in choosing suitable seed sources for reforestation and afforestation programs. A common garden experiment using 240 half-sib families originating from coastal and inland populations of Turkish red pine (Pinus brutia) in Turkey was set up with three replicates. The aims were to determine variation of drought damage, height growth, and phenology among populations and to investigate the relationship between drought damage and physiological traits (i.e. plant moisture stress and proline content). Three-year-old seedlings were subjected to drought treatment during the summer of 2000 and adaptive and physiological traits were measured. Except for bud burst, the majority of the variation resided between populations, leading to low heritability estimates for all traits. On average, inland populations were more resistant to drought and taller, with earlier bud burst and bud set times, than coastal populations. Proline content increased with higher drought damage, especially in cold-resistant and inland families. Inland populations are more drought-resistant than coastal populations. The results of the study demonstrate the possibility of selection for drought resistance for Turkish red pine at the population level.

Variation in the flowering phenology of apple and pear in Australia

Bud burst and flowering dates of commercial apple and pear cultivars were monitored in three geographically and climatically distinct growing regions of Australia (Applethorpe, Queensland; Shepparton, Victoria; Manjimup, Western Australia). Whole tree field assessments were made of 5% green tip, first open flower and progression of flowering to full bloom at all sites. Initial data from 2012 and 2013 indicated significant regional and varietal variation in the timing of bud burst and flowering. Variability in the duration of the period between first open flower and full bloom among sites suggests potential effects of climate on the consistency of flowering. Relationships between phenological variability and temperature are discussed. Field measurements and temperature data are being used to develop cultivar specific models of bud burst and flowering time. These phenological models will be used in conjunction with 2030 climate forecasts to make predictions about potential impacts on the pome fruit industry in Australia. Preliminary results from the modelling analysis are presented.

Non-periodic multi-slit masking for a single counter rotating 2-disc chopper and channeling guides for high resolution and high intensity neutron TOF spectroscopy

Energy resolution is an important design goal for time-of-flight instruments and neutron spectroscopy. For high-resolution applications, it is required that the burst times of choppers be short, going down to the µs-range. To produce short pulses while maintaining high neutron flux, we propose beam masks with more than two slits on a counter-rotating 2-disc chopper, behind specially adapted focusing multi-channel guides. A novel non-regular arrangement of the slits ensures that the beam opens only once per chopper cycle, when the masks are congruently aligned. Additionally, beam splitting and intensity focusing by guides before and after the chopper position provide high intensities even for small samples. Phase-space analysis and Monte Carlo simulations on examples of four-slit masks with adapted guide geometries show the potential of the proposed setup.

Saccadic and Postsaccadic Disconjugacy in Zebrafish Larvae Suggests Independent Eye Movement Control.

Spontaneous eye movements of zebrafish larvae in the dark consist of centrifugal saccades that move the eyes from a central to an eccentric position and postsaccadic centripetal drifts. In a previous study, we showed that the fitted single-exponential time constants of the postsaccadic drifts are longer in the temporal-to-nasal (T->N) direction than in the nasal-to-temporal (N->T) direction. In the present study, we further report that saccadic peak velocities are higher and saccadic amplitudes are larger in the N->T direction than in the T->N direction. We investigated the underlying mechanism of this ocular disconjugacy in the dark with a top-down approach. A mathematic ocular motor model, including an eye plant, a set of burst neurons and a velocity-to-position neural integrator (VPNI), was built to simulate the typical larval eye movements in the dark. The modeling parameters, such as VPNI time constants, neural impulse signals generated by the burst neurons and time constants of the eye plant, were iteratively adjusted to fit the average saccadic eye movement. These simulations suggest that four pools of burst neurons and four pools of VPNIs are needed to explain the disconjugate eye movements in our results. A premotor mechanism controls the synchronous timing of binocular saccades, but the pools of burst and integrator neurons in zebrafish larvae seem to be different (and maybe separate) for both eyes and horizontal directions, which leads to the observed ocular disconjugacies during saccades and postsaccadic drifts in the dark.

MJO simulation in a cloud‐system‐resolving global ocean‐atmosphere coupled model

AbstractAn observed Madden‐Julian Oscillation (MJO) propagating from the central Indian Ocean to the western Pacific from 15 December 2006 to 10 January 2007 was successfully simulated by a cloud‐system‐resolving global ocean‐atmosphere coupled model without parameterization of cumulus convection. We found that the ocean coupling has significant impacts on the MJO simulation, e.g., strength of the moisture convergence, and the timing and strength of the westerly wind burst over the Maritime Continent. The model also generally well simulated the decay of the MJO in the western Pacific, as well as the changes in sea surface temperature. These results demonstrate that the cloud‐system‐resolving global ocean‐atmosphere coupled model can be used for realistic MJO simulation.

A new intuitionistic fuzzy rule-based decision-making system for an operating system process scheduler.

We present a new intuitionistic fuzzy rule-based decision-making system based on intuitionistic fuzzy sets for a process scheduler of a batch operating system. Our proposed intuitionistic fuzzy scheduling algorithm, inputs the nice value and burst time of all available processes in the ready queue, intuitionistically fuzzify the input values, triggers appropriate rules of our intuitionistic fuzzy inference engine and finally calculates the dynamic priority (dp) of all the processes in the ready queue. Once the dp of every process is calculated the ready queue is sorted in decreasing order of dp of every process. The process with maximum dp value is sent to the central processing unit for execution. Finally, we show complete working of our algorithm on two different data sets and give comparisons with some standard non-preemptive process schedulers.

Millihertz quasi-periodic oscillations in 4U 1636−53 associated with bursts with positive convexity only

We investigated the convexity of all type I X-ray bursts with millihertz quasi-periodic oscillations (mHz QPOs) in 4U 1636-53 using archival observations with the Rossi X-ray Timing Explorer. We found that, at a 3.5 $\sigma$ confidence level, in all 39 cases in which the mHz QPOs disappeared at the time of an X-ray burst, the convexity of the burst is positive. The convexity measures the shape of the rising part of the burst light curve and, according to recent models, it is related to the ignition site of bursts on the neutron star surface. This finding suggests that in 4U 1636-53 these 39 bursts and the marginally-stable nuclear burning process responsible for the mHz QPOs take place at the neutron-star equator. This scenario could explain the inconsistency between the high accretion rate required for triggering mHz QPOs in theoretical models and the relatively low accretion rate derived from observations.

Trunk Postural Muscle Timing Is Not Compromised In Low Back Pain Patients Clinically Diagnosed With Movement Coordination Impairments.

Trunk muscle timing impairment has been associated with nonspecific low back pain (NSLBP), but this finding has not been consistent. This study investigated trunk muscle timing in a subgroup of patients with NSLBP attributed to movement coordination impairment (MCI) and matched asymptomatic controls in response to a rapid arm-raising task. Twenty-one NSLBP subjects and 21 matched controls had arm motion and surface EMG data collected from seven bilateral trunk muscles. Muscle onset and offset relative to deltoid muscle activation and arm motion, duration of muscle burst and abdominal-extensor co-contraction time were derived. Trunk muscle onset and offset latencies, and burst and co-contraction durations were not different (p > .05) between groups. Patterns of trunk muscle activation and deactivation relative to arm motion were not different. Task performance was similar between groups. Trunk muscle timing does not appear to be an underlying impairment in the subgroup of NSLBP with MCI.

Twitter user tagging method based on burst time series

Purpose Many Twitter users post tweets that are related to their particular interests. Users can also collect information by following other users. One approach clarifies user interests by tagging labels based on the users. A user tagging method is important to discover candidate users with similar interests. This paper aims to propose a new user tagging method using the posting time series data of the number of tweets. Design/methodology/approach Our hypothesis focuses on the relationship between a user’s interests and the posting times of tweets: as users have interests, they will post more tweets at the time when events occur compared with general times. The authors assume that hashtags are labeled tags to users and observe their occurrence counts in each timestamp. The authors extract burst timestamps using Kleinberg’s burst enumeration algorithm and estimate the burst levels. The authors manage the burst levels as term frequency in documents and calculate the score using typical methods such as cosine similarity, Naïve Bayes and term frequency (TF) in a document and inversed document frequency (IDF; TF-IDF). Findings From the sophisticated experimental evaluations, the authors demonstrate the high efficiency of the tagging method. Naïve Bayes and cosine similarity are particular suitable for the user tagging and tag score calculation tasks, respectively. Some users, whose hashtags were appropriately estimated by our methods, experienced higher the maximum value of the number of tweets than other users. Originality/value Many approaches estimate user interest based on the terms in tweets and apply such graph theory as following networks. The authors propose a new estimation method that uses the time series data of the number of tweets. The merits to estimating user interest using the time series data do not depend on language and can decrease the calculation costs compared with the above-mentioned approaches because the number of features is fewer.

Cerebellar output encodes a corrective saccadic command (Commentary on Sun et al.).

Cerebellar output encodes a corrective saccadic command (Commentary on Sun et al.).

Defining preBötzinger Complex Rhythm- and Pattern-Generating Neural Microcircuits In Vivo

Normal breathing in rodents requires activity of glutamatergic Dbx1-derived (Dbx1(+)) preBötzinger Complex (preBötC) neurons expressing somatostatin (SST). We combined invivo optogenetic and pharmacological perturbations to elucidate the functional roles of these neurons in breathing. In transgenic adult mice expressing channelrhodopsin (ChR2) in Dbx1(+) neurons, photoresponsive preBötC neurons had preinspiratory or inspiratory firing patterns associated with excitatory effects on burst timing and pattern. In transgenic adult mice expressing ChR2 in SST(+) neurons, photoresponsive preBötC neurons had inspiratory or postinspiratory firing patterns associated with excitatory responses on pattern or inhibitory responses that were largely eliminated by blocking synaptic inhibition within preBötC or by local viral infection limiting ChR2 expression to preBötC SST(+) neurons. We conclude that: (1) preinspiratory preBötC Dbx1(+) neurons are rhythmogenic, (2) inspiratory preBötC Dbx1(+) and SST(+) neurons primarily act to pattern respiratory motor output, and (3) SST(+)-neuron-mediated pathways and postsynaptic inhibition within preBötC modulate breathing pattern.

EARLYWOOD VESSELS IN RING-POROUS TREES BECOME FUNCTIONAL FOR WATER TRANSPORT AFTER BUD BURST AND BEFORE THE MATURATION OF THE CURRENT-YEAR LEAVES

This paper reviews the development of xylem vessels in ring-porous dicots and the corresponding leaf phenology. Also included are our original observations on the time-course of vessel element growth, secondary wall deposition, and end wall perforation in the deciduous hardwood Kalopanax septemlobus. Different patterns of xylem growth and phenology serve different strategies of the species for adaptation to seasonal climates. Trees with ring-porous xylem form wide earlywood vessels (EWV) in spring and narrow latewood vessels in summer. The wide EWV become embolized or blocked with tyloses by the end of the growing season while the narrow vessels may remain functional for many years. The co-occurrence of wide and narrow vessels provides both efficiency and safety of the water transport as well as a potentially longer growing season. It has for a long time been assumed that EWV in ring-porous hardwoods are formed in early spring before bud burst in order to supply sap to growing leaves and shoots. However, the full time-course of development of EWV elements from initiation of growth until maturation for water transport has not been adequately studied until recently. Our observations clarify a crucial relationship between leaf maturation and the maturation of earlywood vessels for sap transport. Accumulated new evidence shows that EWV in branches and upper stem parts develop earlier than EWV lower in the stem. The first EWV elements are fully expanded with differentiated secondary walls by the time of bud burst. In lower stem parts, perforations in vessel end walls are formed after bud burst and before the new leaves have achieved full size. Therefore, the current-year EWV network becomes functional for water transport only by the time when the first new leaves are mature.

Characterization and adsorption of Lactobacillus virulent phage P1

Bacteriophage infection of lactic acid bacteria is considered an important problem worldwide in the food fermentation industry, as it may produce low quality or unsafe foods, cause fermentation failure, and result in economic losses. To increase current knowledge on the properties of Lactobacillus virulent phages, we evaluated the effect of divalent cations, temperature, pH, and chloramphenicol on the adsorption ability of Lactobacillus virulent phage P1. Phage P1 was isolated from the abnormal fermentation liquid of Lactobacillus plantarum IMAU10120. The results showed that this phage belonged to the Siphoviridae family. The latent period of this phage was 45min, and the burst time was 90min. Burst size was 132.88±2.37 phage counts expressed per milliliter per infective center. This phage showed good tolerance at different temperatures, but incubation at 50°C only affected its adsorption. Adsorption rate reached a maximum value between 30 and 42°C. A high adsorption value of phage infectivity was obtained from pH 6 to 8. Moreover, calcium ions promoted and increased the adsorption capacity of phage P1, but magnesium ions had negative effects. Chloramphenicol had no effect on phage adsorption. This study increased current knowledge on the characterization and biological aspects of Lactobacillus virulent phages, and may provide some basic information that can be used to design successful antiphage strategies in the food industry.

TBBPA exposure during a sensitive developmental window produces neurobehavioral changes in larval zebrafish

Tetrabromobisphenol A (TBBPA), one of the most widely used brominated flame retardants (BFRs), is a ubiquitous contaminant in the environment and in the human body. This study demonstrated that zebrafish embryos exposed to TBBPA during a sensitive window of 8–48 h post-fertilization (hpf) displayed morphological malformations and mortality. Zebrafish exposed exclusively between 48 and 96 hpf were phenotypically normal. TBBPA was efficiently absorbed and accumulated in zebrafish embryos, but was eliminated quickly when the exposure solution was removed. Larval behavior assays conducted at 120 hpf indicated that exposure to 5 μM TBBPA from 8 to 48 hpf produced larvae with significantly lower average activity and speed of movement in the normal condition than in those exposed from 48 to 96 hpf. Specifically, 8–48 hpf-exposed larvae spent significantly less time in both activity bursts and gross movements compared to control or 48–96 hpf exposed larvae. Consistent with the motor deficits, TBBPA induced apoptotic cell death, delayed cranial motor neuron development, inhibited primary motor neuron development and loosed muscle fiber during the early developmental stages. To further explore TBBPA-induced developmental and neurobehavioral toxicity, RNA-Seq analysis was used to identify early transcriptional changes following TBBPA exposure. In total, 1969 transcripts were significantly differentially expressed (P < 0.05, FDR < 0.05, 1.5-FC) upon TBBPA exposure. Functional and pathway analysis of the TBBPA transcriptional profile identified biological processes involved in nerve development, muscle filament sliding and contraction, and extracellular matrix disassembly and organization changed significantly. In addition, TBBPA also led to an elevation in the expression of genes encoding uridine diphosphate glucuronyl transferases (ugt), which could affect thyroxine (T4) metabolism and subsequently lead to neurobehavioral changes. In summary, TBBPA exposure during a narrow, sensitive developmental window perturbs various molecular pathways and results in neurobehavioral deficits in zebrafish.

Two-Stage Resource Allocation for Random Access M2M Communications in LTE Network

Deployment of massive machine-to-machine (M2M) user equipments (UEs) in the current cellular network may cause overload in the radio access network (RAN). Access class barring (ACB) is an effective solution for reducing the RAN overload. In this letter, we propose an extended random access (RA) scheme to increase access success probability of M2M UEs by efficient use of available uplink radio resources. The proposed scheme allocates the available radio resources to the access-attempting UEs in two stages. In the first stage, the evolved node B (eNB) grants the available uplink resources to the UEs that have passed the ACB check. Then in the second stage, UEs that did not pass the ACB check utilize the remained unscheduled resources from the first stage. Simulation results show that the proposed scheme increases the number of successful requests and decreases the total service time of a traffic burst.

Population-Level Representation of a Temporal Sequence Underlying Song Production in the Zebra Finch

The zebra finch brain features a set of clearly definedand hierarchically arranged motor nuclei that are selectively responsible for producing singing behavior. One of these regions, a critical forebrain structure called HVC, contains premotor neurons that are active at precise time points during song production. However, the neural representation of this behavior at a population level remains elusive. We used two-photon microscopy to monitor ensemble activity during singing, integrating across multiple trials by adopting a Bayesian inference approach to more precisely estimate burst timing. Additionally, we examined spiking and motor-related synaptic inputs using intracellular recordings during singing. With both experimental approaches, we find that premotor events do not occur preferentially at the onsets or offsets of song syllables or at specific subsyllabic motor landmarks. These results strongly support thenotion that HVC projection neurons collectively exhibit a temporal sequence during singing that is uncoupled from ongoing movements.

Continuous Time Representations of Song in Zebra Finches

Neurons in the songbird nucleus HVC produce premotor bursts time locked to song with millisecond precision. In this issue of Neuron, Lynch etal. (2016) and Picardo etal. (2016) provide convincing evidence that the population of these bursts contain a continuous representation of time throughout song.

Investigation on spalling resistance of ultra-high-strength concrete under rapid heating and rapid cooling

Effects of the fiber type, dosage and length on the explosive spalling of ultra-high-strength concrete under rapid heating and rapid cooling were experimentally investigated. The mechanism of spalling resistance was examined by comprehensive thermal analysis, X-ray diffraction analysis, scanning electron microscopy and mercury porosimetry. The burst time is extended but the spalling is unaffected by the addition of steel fiber. The spalling resistance is improved with the addition of polypropylene (PP) fiber or PP and steel fibers. Ultra-high-strength concrete with 0.20% (vol.) PP fiber has excellent spalling resistance. The resistance to explosive spalling is enhanced with 12- or 19-mm-long PP fibers. PP fiber improves the spalling resistance mainly through the formation of tubular channels.