The perceptual dependency of the timbre of musical sounds on their fundamental frequency (F0) was examined through two timbral dissimilarity experiments. In Experiment 1, subjects judged timbral dissimilarity between pairs of stimuli produced by 12 musical instruments equal in F0, duration, and loudness. There were three sessions, each at a different F0 (B2, Db3, Bb3). In Experiment 2, the same stimuli were presented in pairs with a constant difference in F0. Two sessions corresponded to two different intervals (B2-Db3 and B2-Bb3). Subjects were instructed to ignore pitch difference. The results are interpreted in terms of an evolution of timbre space as a function of F0. Experiment 1 produced dissimilarity matrices that were similar at different F0s, suggesting that the relative positions of instruments within the timbre space varied little with F0. Experiment 2 produced dissimilarity matrices that were symmetrical, suggesting further that F0-dependent displacements within the timbre space are small. Patterns of dissimilarity were similar with or without an F0 difference, showing that subjects could ignore a constant difference in pitch when making timbral dissimilarity judgments. Results suggest that the perception of timbre is relatively independent of pitch and can be abstracted across differences in F0 under these conditions.