Sesame Oil Research Articles

Analysis of the Generation of Harmful Aldehydes in Edible Oils During Sunlight Exposure and Deep-Frying Using High-Field Proton Nuclear Magnetic Resonance Spectroscopy

Edible oils are essential dietary components that provide crucial micronutrients. However, their quality can deteriorate during frying—a common cooking method—and with prolonged light exposure due to chemical reactions such as hydrolysis, oxidation, and polymerization. These processes lead to the formation of harmful compounds, particularly aldehydes. This study investigates how thermal and light exposure impact the chemical composition of five widely used edible oils: olive, rapeseed, sunflower, sesame, and peanut oils. For the thermal treatment, the oils were heated to 190 ± 5 °C in a commercial fryer, with samples taken at the start and after 10 min and 60 min of heating, while intermittently frying chicken nuggets to simulate typical frying conditions. For the light exposure treatment, the oil samples were exposed to direct sunlight for 3 and 8 h, with control samples being collected beforehand. The oil composition was analyzed using an advanced 800 MHz nuclear magnetic resonance (NMR) instrument with a triple-resonance inverse cryoprobe, providing high sensitivity and resolution. The results revealed a significant increase in various aldehyde compounds in all oils under both thermal and light exposure conditions. Notably, this study identified the generation of genotoxic and cytotoxic α,β-unsaturated aldehydes, including 4-hydroperoxy-(E)-2-alkenals, 4-hydroxy-(E)-2-alkenals, and 4,5-epoxy-(E)-2-alkenals. Given the established association of aldehydes with health risks, including cancer, Alzheimer’s, and Parkinson’s diseases, these findings highlight the importance of monitoring oil degradation during cooking and the appropriate storage of oils to minimize light exposure to reduce potential health risks.

Evaluation of Bilva Taila prepared on the principle of Snehapaka through Quality Control Metrics

The globalization of Ayurveda has led to an increased focus on the standardization and quality control of traditional formulations like Sneha Kalpana. It involves the process of infusing fats with herbal properties to enhance their therapeutic effects. Certain principles were given by Acharya Sharangdhara for the preparation of Sneha Kalpana. Bilva Taila was taken as an example of Sneha Kalpana and two samples were prepared according to the Snehapaka principles by two different references. This study aims to evaluate Snehapaka principle through the quality control metrics of Bilva Taila. The organoleptic characters, physico-chemical parameters, phyto-chemical screening, Marmelosin quantification through HPTLC analysis and heavy metal analysis of raw material i.e. Apakva Shushka Bilva Phala (Unripe Bael fruit powder), Tila Taila (Sesame oil) and finished products (Both the samples of Bilva Taila) were done. The results showed that the pH value for all three samples of Taila was similar, while all other physico-chemical parameters varied between samples. Phyto-chemical screening revealed the presence of various functional group in different samples. Marmelosin quantification also vary in different samples. Heavy metal analysis confirmed that all samples met the permissible limits for lead, arsenic and mercury, indicates the quality and safety for therapeutic use. The study concludes that there is difference found in preliminary analysis, Phyto-chemical screening and marmelosin quantification of both the samples of Bilva Taila prepared on the principle of Snehapaka.

Role of Nimba Tail and Tiladi Tail Nasya in Khalitya - A Review

Khalitya, commonly known as hair loss or baldness, is a prevalent condition that affects both men and women, often leading to psychological distress and a decrease in quality of life. In Ayurvedic medicine, the management of Khalitya involves the use of various herbs and oils to restore hair health and balance Doshas. Among these, Nimba Tail (Neem oil) and Tiladi Tail (a sesame oil-based formulation) Nasya have gained significant attention due to their therapeutic properties. Nimba Tail, known for its antibacterial, anti-inflammatory, and detoxifying effects, helps in treating scalp infections, reducing dandruff, and promoting overall hair growth by balancing Pitta and Kapha Doshas. Tiladi Tail, a combination of sesame oil and medicinal herbs, nourishes the hair follicles, improves circulation to the scalp, and strengthens the roots, aiding in the prevention of hair fall. Both oils are often used topically to rejuvenate the scalp, reduce excess heat, and enhance hair growth. This abstract explores the role of Nimba Tail and Tiladi Tail Nasya in managing Khalitya by addressing the root causes of hair loss through their synergistic effects on the scalp's health, follicular nourishment, and Dosha balance. The therapeutic potential of these oils, when used as part of a holistic Ayurvedic treatment approach, offers promising results in the prevention and management of hair loss.

Neuroprotective Effect of Sesame Oil Against Radiation-Induced Alzheimer-Like Symptoms in Rats: Involvement of IL-6 and Hepcidin Regulatory Pathway

Neuroprotective Effect of Sesame Oil Against Radiation-Induced Alzheimer-Like Symptoms in Rats: Involvement of IL-6 and Hepcidin Regulatory Pathway

Anti-Inflammatory and Wound Healing Potential of Comphora Wightii,Herbal Ointment on Wistar Rats

Background: A herbal approach to Guggulu (Commiphora wightii), as an anti-inflammatory and wound healing agent is anticipated. Phyto-constituents Guggulusterone, Naringenin, and myrrhanol were reported for the anti-inflammatory activity of Guggulu. Studies suggests, that sesame oil (Sesamum indicum L.) and Ratanjot (Arnebia nobilis) also act as potent anti-inflammatory agents.. A combination of all three of these gives a synergistic effect for anti-inflammatory and excisional wound healing activity. Materials and Methods: A simple ointment base, BP and 5% Ratanjot, and Sesame oil were prepared. All the components, i.e., Guggulu, 5% Ratanjot Sesame oil, and simple ointment base, were added and triturated uni-directionally until a smooth, homogenous mixture was obtained. 25% w/w (F1) and 27% w/w (F2) Guggulu ointment were prepared. Four groups, each with three wistar rats, were studied for fourteen days. On the fourteenth day, rats were sacrificed, and tissues were collected for histopathological studies. Results: F1 was compared against the standard formulation (10% w/w betadine, Win-Medicare) for excisional wound healing and anti-inflammatory activity in rats. On the foutheenth day, the results for percentage wound contraction in groups 1 (Negative control, vaseline), 2 (Controlled, ointment base), 3 (F1), and 4 (positive control, standard) were found to be 12.01, 25.32, 96.14, and 81.23, respectively. Results of histopathological studies and H&E staining supported the action of F1, as skin sections showed the junction of normal skin and wound area. Sub-epithelial tissues showed low (-) to mild (+) inflammation. Mild oedema (+) was also noted. Conclusion: The rat group treated with F1 showed maximum wound contraction, healing, and antiinflammatory activity as per percentage wound contraction and histopathological studies.

Partial discharge characteristics of thermally aged mineral oil and eco-friendly vegetable oil under uniform electric field

Partial discharge (PD) detection plays an important role in the life assessment of liquid insulation in transformers. This paper investigates on the PD activity and characteristics in thermally aged mineral oil and eco-friendly rice bran and Sesame oils. To simulate surface discharges, plane-plane electrode configuration with pressboard of 1mm and 3mm thickness was used. The phase resolved PD patterns of thermally aged rice bran oil, sesame oil and mineral oil with pressboard were recorded and analyzed. From the results it is observed that the inception voltage of eco-friendly vegetable oils is higher than mineral oil. Also vegetable oil shows better PD characteristics hence they can be used for high voltage insulation applications.

Microchannel fabrication on bio-grade Nitinol SMA by μ-ED milling process using sustainable oil for improving the machining performance and biocompatibility

The process of micromachining has garnered attention for its ability to create three-dimensional tiny features, particularly in ultra-hard and exotic materials. The present work investigates the effect of different parameters of theμ-ED milling, such as pulse on time (Ton), pulse off time (Toff), voltage (V), and tool rotation (TR) on the dimensional deviation (DD), material removal rate (MRR), surface roughness (Ra), and machined surface characteristics (analyzed by EDS and FESEM). The sesame oil as dielectric and tungsten-copper as tool electrodes were used to maintain the accuracy and improve the machinability of bio-grade Nitinol shape memory alloy (SMA). Response surface methodology (RSM) and genetic algorithms (GAs) were used to optimize the various input parameters of theμ-ED milling process. Artificial neural network was combined with GA to find the best parametric combination for microchannel fabrication. The cytotoxicity test was also performed on the machined surface to analyze the biocompatibility of the machined surface. It was found that the cell viability of Nitinol SMA was improved by 85.11% after machining at the optimum condition. The highest MRR was found to be 0.076 gm min-1, and the lowest DD and Ra were found to be 16.47μm and Ra 0.387μm, respectively.

Effect of Various Oils on the Production of Lipase by Rhodotorula mucilaginosa and Yarrowia lipolytica Isolated from Spoiled Labneh

The content of the medium is very important in the production of lipase enzyme. Microbial lipase production is carried out by adding carbon source to the compounds in the minimal medium. The use of olive oil is quite common in lipase production medium. In this study, it was aimed to increase lipase production by adding different oils instead of olive oil to the reference lipase production medium. For this purpose, corn oil, sunflower seed oil, walnut oil, hazelnut oil, safflower oil, avocado oil, organic sunflower seed oil, organic canola seed oil, organic flaxseed oil, organic soybean oil, pumpkin seed oil, black cumin oil, St. John’s wort oil and sesame oil were evaluated in terms of lipase production. Rhodotorula mucilaginosa and Yarrowia lipolytica strains, which were isolated, purified, and identified, and which caused spoilage in labneh were studied in lipase production. When olive oil was used in lipase production, the lipase activity of R. mucilaginosa was determined as 7.50 U/ml, and that of Y. lipolytica was determined as 8.09 U/ml. It was determined that the lipase activity of R. mucilaginosa was increased by organic linseed oil as 10.67 U/ml, St. John’s Wort oil as 10.17 U/ml, and organic sunflower oil as 9.67 U/ml, respectively. It was determined that the lipase activity of Y. lipolytica was increased by St. John’s Wort oil as 13.50 U/ml, organic linseed oil as 10.84 U/ml, and organic sunflower oil as 10.33 U/ml, respectively. It was also observed that black cumin oil inhibited the growth of both yeast strains.

Theoretical Modelling, Experimental Testing and Simulation Analysis of Thermal Properties for Green Building-Insulation Materials

In this study, 45 alternative green materials for building walls were experimentally produced, utilizing renewable (epoxidized sesame oil), natural (clay), and waste (Seyitömer fly ash) resources. These materials were evaluated based on key technical properties such as mass, tensile-compressive strength, and thermal conductivity, all of which are essential for construction and insulation applications. Subsequently, theoretical modeling was conducted for the material coded SE45, which demonstrated the lowest thermal conductivity. Through mathematical calculations, the theoretical thermal conductivity value was determined with a deviation of +5.88%. Furthermore, 48 alternative scenarios were designed for three different building envelope types (internally insulated, externally insulated, and sandwich), using commonly used building insulation materials alongside the sesame oil-based green material with the lowest thermal conductivity (SE45). Energy performance evaluations were conducted by analyzing temperature distributions along the walls of all designed scenarios using ANSYS simulations under the climatic conditions of Ankara, Turkey.

Hybrid Preceramic Aerogels for Oil and Solvent Cleanup

This study presents the first synthesis and characterization of monolithic hybrid preceramic aerogels using distinct drying techniques: ambient pressure (ambigels) and CO2 supercritical drying. Polymeric ambi/aerogels, derived from polyhydromethlysiloxane (PHMS) and divinylbenzene (DVB), are processed at 200 °C, while hybrid ceramic‐polymer (ceramer) is produced through pyrolysis at 600 °C. Despite variations in drying methods, polymer and ceramer ambi/aerogels exhibit comparable microstructural characteristics, bulk density, pore size and volume, and specific surface area (542–841 m2 g−1). Polymeric and ceramer ambigel with 90 vol% total porosity yield a compressive strength, reaching 2.5 MPa, demonstrating a low thermal conductivity of 0.046 W m−1 K−1. Sorption tests are conducted using oil and organic solvents in aqueous media to benefit their high hydrophobicity (112° < θ < 142°). Aerogels exhibit high sorption capacities: 13.17 g g−1 for sesame oil, 11.74 g g−1 for toluene, and 9.19 g g−1 for n‐hexane. The sorption rate for the oil is nearly 10 times slower than that for toluene and n‐hexane. Regarding regeneration and reusability, polymer and ceramer aerogels show consistent sorption properties cycles tested for n‐hexane and toluene.

Acid-catalyzed conversion of sesamolin to sesamol: Kinetics and reaction mechanism based on density functional theory.

Sesamol is a significant lignan in sesame oil, which can be converted from sesamolin under acid-catalyzed conditions. The effects of several factors on the conversion of sesamolin to sesamol under acid-catalyzed conditions were investigated. The conversion kinetics were studied and the relevant conversion mechanism was revealed by density functional theory (DFT). The results indicated that catalyst dosage and substrate concentration had significant effects on the yield of sesamol. The conversion of sesamolin conformed to the characteristics of a first-order kinetics reaction within the range of 50-70°C. The activation energy of the reaction was 35.78±1.21kJ/mol. The energy barrier to be crossed for the formation of intermediates in generating sesamol was 237.83kJ/mol; the energy barrier to be overcome for the formation of intermediates in generating sesaminol was 266.95kJ/mol. This study provides information relevant to increasing the percentage of sesamol in processed sesame oil.

Determination of the Sesame Oil Biodiesel (SOB) Ratio Providing the Lowest Emissions by Multi-Purpose RSM Optimization

Emissions from internal combustion engine vehicles have a major impact on environmental pollution and global warming, which are among the world's biggest problems. The use of alternative fuels is quite popular to reduce the emission values originating from diesel engines, which are preferred due to their high efficiency. Another issue that has become popular in recent years is optimization studies for alternative fuels. In this study, to determine the most suitable sesame oil biodiesel (SOB) in terms of emissions in a single cylinder diesel engine using SOB as an alternative fuel, firstly engine experiments were performed, and response surface methodology (RSM) optimization was performed using experimental data. In the optimization design, SOB percentage and engine load were determined as factors, while carbon monoxide (CO), hydrocarbon (HC), carbon dioxide (CO2), and nitrogen oxide (NOx) were determined as responses affected by the factors. The optimum variable levels offered by the optimization study are 15% SOB and 850 W engine load. The emission levels designed as responses under these conditions are 0.0680% CO, 7.1858 ppm HC, 4.0887% CO2, and 316.4166 ppm NOx. When compared with the test results, it was concluded that the RSM results and the test results converged in the 0.71%-2.34% error range and accordingly the RSM optimization was successfully performed.

Physico-Chemical Characterization of Sesame/Rapeseed Oil Mixtures

The production of edible plant seed oil used in the food industry is increasing globally. More than 75% of lipids in the human diet come from edible vegetable oils. Among them, sesame oil has the highest resistance to oxidation, valuable physiological properties, and a unique flavor and aroma. However, sesame oil is more expensive than rapeseed oil, and often both oils are mixed to reduce costs. In this study, we performed a physical and physico-chemical analysis of sesame oil and sesame/rapeseed oil mixtures (5/95, 10/90, 30/70, 50/50, 70/30 and 90/10 w/w). The investigated oils were characterized based on their fatty acid composition, peroxide value, iodine value, phase transitions, refractive indices, color and UV–Vis adsorption. The fatty acid composition of mixtures made from sesame and rapeseed oils depended on the ratio of the two oils. Increasing the content of sesame oil in the mixtures resulted in a decrease in the levels of oleic and linolenic acids, while the levels of linoleic acid increased. A very good linear correlation was observed between the temperatures and enthalpies of crystallization of the mixtures, which could be used to establish the composition of a mixture between sesame and rapeseed oil. Information about these parameters could increase the possibilities for the commercial use of the investigated oils.

The plaque reducing efficacy of oil pulling with sesame oil: a randomized-controlled clinical study

ObjectivesTo compare the plaque reducing efficacy of oil pulling with sesame oil compared to distilled water in a randomized, controlled, examiner-blinded parallel group study.Materials and methodsForty probands without advanced periodontal disease of the University Hospital for Restorative Dentistry and Periodontology, Medical University of Innsbruck (Austria) were randomized allocated to test- (sesame oil) or control group (distilled water) and asked to pull daily in the morning for eight weeks with their allotted fluid for 15 min. Rustogi Modified Navy Plaque Index (RMNPI) and gingival bleeding index were assessed at baseline and after four and eight weeks. Plaque samples underwent microbiological analysis.ResultsPulling with sesame oil was significantly more effective in reducing full mouth RMNPI compared to distilled water after eight weeks (median reduction 18.98% versus 10.49%; p = 0.023), and was most pronounced in anterior, buccal, and lingual subscales. On approximal surfaces, significantly higher plaque reduction was found in the test group after four (24.07% versus 14.29%) and eight weeks (16.00% versus 5.36%) of intervention (p < 0.05). No significant changes in gingival index and mirobiological analysis could be detected.ConclusionPlaque reduction was statistically significantly higher with oil pulling than with distilled water, however, a study bias cannot be ruled out. Further high-quality trials are needed to understand the mechanisms and effectiveness of oil pulling, to finally clarify the evidence.Clinical relevanceOil pulling may be recommended as an adjuvant to mechanical dental cleaning. Individuals with keratosis may experience adverse effects.Trial registrationClinicalTrials.gov NCT06327841.

Transcriptomic analysis of effects of developmental PCB exposure in the hypothalamus of female rats.

This study investigated the consequences of perinatal exposure to Aroclor 1221 (A1221), a weakly estrogenic polychlorinated biphenyl (PCB) mixture and known endocrine-disrupting chemical (EDC), in female rats. Previous work has shown behavioral and physiological effects of A1221, and the current study extended this work to comprehensive transcriptomic profiling of two hypothalamic regions involved in the control of reproduction: the arcuate nucleus (ARC) and anteroventral periventricular nucleus (AVPV). Female Sprague-Dawley rats were fed a cookie treated with a small volume of A1221 (1mg/kg) or vehicle (3% DMSO in sesame oil) during pregnancy from gestational days 8-18 and after birth from postnatal (P) days 1-21, exposing the offspring via placental and lactational transfer. In female offspring, developmental, physiological, and hormonal effects of A1221 were relatively modest. However, because prior work has implicated this exposure in neurobehavioral disruptions, we sought to determine whether developmental programming of the brain transcriptome could underlie these latter phenotypes. We used 3' targeted RNA sequencing in the hypothalamus (arcuate nucleus, anteroventral periventricular nucleus) of experimental females at P8, 30, and 60 and identified significant alterations in gene expression and gene ontology (GO) terms in an age- and tissue-specific manner. Most notably, terms related to synaptic signaling, neurotransmitter regulation, immune response, and cellular structure were identified. Changes in pathways associated with synaptic functions and cellular metabolism were further identified, indicating that A1221 exposure can impact neurodevelopmental and neuroendocrine processes at a molecular level, even in the absence of overt developmental changes. These findings of molecular reprogramming may explain the behavioral effects of A1221 and highlight novel molecular targets and pathways that warrant further investigation to understand the effects of EDCs on the developing brain.

Edible Nanocoating of Dextran/Lipid and Curcumin for Enhanced Shelf Life of Fresh Produce

ABSTRACTThe purpose of the present study was to develop a nano‐coating for fresh vegetables and fruits using a combination of curcumin, sesame oil, stearic acid, and dextran (CSSDNs). The anti‐bacterial and nontoxic nature of nanoparticles were tested against Gram‐positive bacteria (Staphylococcusaureus) and human fibroblast cells. Enhanced aqueous solubility of curcumin and its stability in the bloodstream were observed from the in vitro blood compatibility studies. The anti‐cancer activity of curcumin‐loaded nanoparticles was tested against SK‐BR3 cells, demonstrating excellent antitumor activity. These findings indicate that the CSSDNs system can be used as an edible coating with strong antibacterial properties and as an effective means of delivering curcumin, which has proven to be successful in treating or preventing cancer.

Decoding gene expression dynamics during seed development in sesame (Sesamum indicum L.) through RNA-Seq analysis.

Sesame (Sesamum indicum L., 2n = 2× = 26) from the Pedaliaceae family is primarily grown for its high oil content, rich in unsaturated fatty acids like linoleic acid (LA) and alpha-linolenic acid (ALA). However, the molecular mechanisms of sesame oil accumulation remain poorly understood. This study analyzed transcriptomes at two seed development stages: Young Stage (YS, pods 1.5-2.5 cm) and Mature Stage (MS, brown pods >2.5 cm), to explore regulatory mechanisms and identify key genes involved in lipid biosynthesis. From 25,173 genes, 18,820 with expression values >10 CPM in at least 70 % of replicates were included in differential expression (DE) analysis. Active expression (LFC > 0) was observed in 9372 and 9448 genes at YS and MS, respectively. DEGs were annotated, revealing roles in various biological processes, (e.g., mRNA metabolic process, reproduction-related developmental processes, seed development), molecular functions (e.g., aminoacyltransferase activity, ubiquitin-like protein and ubiquitin-protein transferase activities), and cellular components (e.g., peroxisome, microbody, lipid droplet). KEGG analysis highlighted genes involved in fatty acid synthesis (e.g., fabG, fabZ), TAG biosynthesis (DGAT1, GPAT), and alpha-linolenic acid metabolism (AOS, LCAT3). Key genes upregulated at MS included SIN_1025205 (protein transport), SIN_1006853 (acetylajmalan esterase), and SIN_1003267 (gamma-cadinene synthase). The study generated a valuable transcriptome dataset and gene list for seed development and lipid biosynthesis, which will be validated through functional studies. An interactive webpage is provided for data exploration.

Mixed diesel fuel with additive sesame oil esters for diesel engines

Currently, the issue of expanding the raw material base to increase the production of diesel fuels and improve their environmental performance is a relevant and important area. One of the types of raw materials for the production of alternative types of biofuels for diesel engines are vegetable oils. The use of bioadditives can reduce the emission of harmful substances compared to the use of petroleum diesel fuels. In this article, the features of using diesel fuels with the addition of mixtures of methyl esters of sesame oil in diesel engines are studied for the first time. Mixtures of fatty acid esters of sesame oil were obtained by direct transesterification of sesame oil with methyl alcohol at a temperature of 80-90 °C in the presence of potassium hydroxide. The results of the study established for the first time that the addition of sesame oil and methyl esters synthesized on its basis as additional components of diesel fuel in the amount of 3 and 5% by weight, respectively, improves the cetane number of diesel fuel by up to 7 points, as well as reduces emissions of harmful substances with exhaust gases by 25% rel. Some weight gain of the fuel is observed ‒ the values of density, kinematic viscosity, concentration of actual resins and iodine number increase. It is shown that the production of diesel fuel with the addition of mixtures of methyl esters of sesame oil allows to significantly expand resources and obtain diesel fuels with improved environmental characteristics. Keywords: diesel fuel; sesame oil; sesame oil esters; environmental characteristics.

Nanocellulose-based Pickering emulsion of sesamolin manifested increased anticancer activity and necrosis in human colon cancer (HCT116) cells.

Sesamolin possesses limited aqueous solubility, a drawback for biological activity study in cancer cell models. This study aimed to enhance sesamolin's ability to fight cancer, as it is a bioactive compound with low water solubility found in sesame. We developed different Pickering emulsion delivery systems and tested their anticancer effects on various cancer cell types. Sesamolin was incorporated into either sesame or olive oil and subsequently formulated as oil in water (o/w) Pickering emulsions stabilized by the carboxylated cellulose nanocrystal (cCNC). The anticancer activity was determined based on cell viability and the induction of cell death mechanisms. The results demonstrated a synergistic effect of the components in the emulsion, including sesamolin, sesame oil, and olive oil, and a decrease in HCT116 viability in a concentration-dependent manner and selectively on cancer cells compared to non-cancerous Vero cells. The primary mode of cell death was predominantly ROS-induced necrosis, with no change in caspase 3/7 activity, indicating the absence of apoptosis. This study first presents the necrotic cell death mechanism induced by sesamolin. The findings reveal that the cCNC emulsion delivery system is safe and appropriate for transporting lipophilic chemicals and can overcome solubility limitations.

Exploring the influence of different vegetable oils on the stability of nanoemulsions in the presence and absence of nisin and limonene

AbstractBACKGROUNDContamination during food production and handling is a major health concern. Traditional preservation methods often influence food quality as well as their organoleptic characteristics. In contrast, natural alternatives like bacteriocins and essential oils are emerging. Nisin, a natural antimicrobial agent, is effective against Gram‐positive bacteria but not against Gram‐negative bacteria. Moreover, essential oils like limonene, although they have broad antimicrobial properties, are not water‐soluble and affect the sensory properties. Encapsulation in oil‐in‐water nanoemulsions offers a promising approach, enhancing the stability, solubility, and functionality of these natural antimicrobial agents.RESULTSDifferent concentrations of olive, corn, and sesame oils were used in the presence or absence of nisin and limonene. Parameters such as droplet size, polydispersity index, zeta potential, and cream index were assessed. Oil type and concentration played a crucial role in the stability and properties of produced nanoemulsions. Sesame oil‐based emulsions showed superior stability, while olive oil‐based emulsions performed well at lower concentrations. Nisin reduced droplet size, while limonene increased it, with combined use yielding intermediate results.CONCLUSIONThe properties and stability of oil‐in‐water nanoemulsions using olive, corn, and sesame oils at varying concentrations, with and without nisin and limonene, were investigated. Sesame oil‐based nanoemulsions exhibited stable droplet size and lower polydispersity index values, while nisin reduced droplet size and limonene increased it. Stability assessments favoured olive oil‐based formulations at low oil concentrations (2% v/v). These findings provide insights into optimizing nanoemulsion formulations for food and pharmaceutical applications. © 2024 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley &amp; Sons Ltd on behalf of Society of Chemical Industry (SCI).