Tiamulin Research Articles

A study of tiamulin removal by nanosecond pulsed gas–liquid discharge underwater

AbstractIn this study, gas–liquid discharge plasma excited by nanosecond pulsed voltage is used to efficiently remove tiamulin (TIA) from water. The discharge produces a large number of reactive species (H2O2, OH radicals, NO3−, etc.) that can attack the TIA molecules. The effects of peak pulse voltage, initial TIA concentration, gas composition, and the addition of ferrous sulfate and persulfate on TIA removal were mainly investigated. The results showed that the oxygen plasma could approach 100% removal within 30 min of treatment time. The addition of the catalyst increased the TIA removal efficiency by approximately 15% during the 10‐min discharge treatment time. The toxicity of 12 intermediates was analyzed and the degradation mechanism of TIA was investigated.

Bioaugmentation of manures by a tiamulin-degrading Sphingomonas as a means to alleviate environmental dispersal of antibiotic residues

Bioaugmentation of veterinary antibiotic (VA)-contaminated feces with VA-degrading bacteria, can reduce the VA-selective pressure on manure microorganisms toward antibiotic resistance during organic soil fertilization. We explored the efficiency of a Sphingomonas isolate, able to degrade tiamulin (TIA), as a bioaugmentation agent for removing TIA from pig manures fortified with TIA at two concentrations (5 and 50 µg g−1). Bioaugmentation efficiency was compared with stockpiling and anaerobic digestion. Bioaugmentation accelerated TIA dissipation (DT50 = 32.3 and 66.2 days) compared with anaerobic digestion (DT50 = 103 and 126.7 days) and stockpiling (DT50 = 95.38 and 113.8 days). Furthermore, we determined the impact of TIA on methane production and followed microbial succession during anaerobic digestion. The lower TIA concentration stimulated biomethanation, seemingly, through induction of the increase of Methanosarcina (from below detection at the TIA-free samples to 17.1% of relative abundance at the low in TIA content samples), commonly linked with aceticlastic biomethanation. Our findings highlight (i) the potential of bioaugmentation in treating VA-contaminated manures for mitigating the dispersal of VA residues in agricultural settings (ii) the high risk of contamination of agricultural soils by the use of TIA-contaminated manures that have been stockpiled or anaerobically digested before their use in agricultural settings.

Exploration of Cytochrome P450-Related Interactions between Aflatoxin B1 and Tiamulin in Broiler Chickens.

Poultry may face simultaneous exposure to aflatoxin B1 (AFB1) and tiamulin (TIA), given mycotoxin contamination and antibiotic use. As both mycotoxins and antibiotics can affect cytochrome P450 enzymes (CYP450), our study aimed to explore their interaction. We developed UHPLC-MS/MS methods for the first-time determination of the interaction between TIA and AFB1 in vitro and in vivo in broiler chickens. The inhibition assay showed the half maximal inhibitory concentration (IC50) values of AFB1 and TIA in chicken liver microsomes are more than 7.6 μM, indicating an extremely weak inhibitory effect on hepatic enzymes. Nevertheless, the oral TIA pharmacokinetic results indicated that AFB1 significantly increased the area under the plasma concentration-time curve (AUClast) of TIA by 167% (p < 0.01). Additionally, the oral AFB1 pharmacokinetics revealed that TIA increased the AUClast and mean residence time (MRT) of AFB1 by 194% (p < 0.01) and 136%, respectively. These results suggested that the observed inhibition may be influenced by other factors, such as transport. Therefore, it is meaningful to further explore transport and other enzymes, involved in the interaction between AFB1 and TIA. Furthermore, additional clinical studies are necessary to thoroughly assess the safety of co-exposure with mycotoxins and antibiotics.

Advanced suspect screening of tiamulin and its transformation products in waters: Assessing their persistence, mobility and toxicity

Persistent, Mobile, Organic Compounds (PMOCs) exhibit a propensity to disseminate within the water cycle and as a result, pose a significant threat to the quality of drinking water. To this point of view, considerable research has been initiated to explore the environmental behavior of the omnipresent tiamulin (TIA), being potential PMOC. In contrast to the parent compound (TIA), its transformation products (TPs) have received relatively limited attention, particularly in the context of their PM (persistent-mobile) characteristics. To address this research gap, an investigation into the phototransformation of TIA was conducted, through solar-simulated experiments in combination with Advanced Oxidation Processes (AOPs). To this end, an innovative suspect-screening methodology, involved comparison of the results obtained for the identified photo-TPs with existing literature data. This enabled us to construct an enhanced high-resolution mass spectrometry (HRMS) database for the purpose of environmental assessments of TIA and its TPs in the environment. In total, 32 TPs were identified, with 18 of them being pioneeringly reported. The principal transformation pathways appeared to involve hydroxylation, oxidation, dealkylation, and the cleavage of the C-O bond. Additionally, a comprehensive monitoring of TIA and its TPs in various environmental compartments, including samples from livestock units, leachates, surface water, and groundwater was accomplished. The highest concentration was observed in solid samples from the livestock unit at 2847 ng/g. Furthermore, both the in silico and in vitro methods to assess the toxicity of TIA and its TPs were performed. The results indicated that TIA and many TPs exhibit an alarming toxicity. Consequently, TIA can be classified as a potential PMOC, with a heightened potential for occurrence in different environmental matrices compared to ascertained PM chemicals.

A UPLC-MS/MS method for simultaneous determination of tiamulin and its metabolites in Crucian carp (Carassius carassius): an in vivo metabolism and tissue distribution study

Tiamulin (TML) has been studied and analyzed in pigs, cattle, chickens, ducks, and other domestic animals, however, its metabolic state in fish has not been well explored. This study investigated TML metabolism in Crucian carp (Carassius carassius). After intraperitoneal injection of TML into Crucian carp, ultra-high performance liquid chromatography with quadrupole and time-of-flight mass spectrometry (UPLC/Q-TOF MS) analysis, was conducted to identify TML metabolites. The UPLC/Q-TOF MS analysis and the relative molecular mass of the metabolites obtained from related literature identified five metabolites in Crucian carp. These metabolites were M1 (510.2908, C28H48NO5S+), M2 (510.2908, C28H48NO5S+), M3 (466.2750, C26H44NO4S+), M4 (482.2663, C26H44NO5S+), and M5 (482.2663, C26H44NO5S+). The enrichment and metabolism of TML and its metabolites in Crucian carp were investigated using the drug bath method combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). TML exhibited an overall trend of an initial increase followed by a decrease. Moreover, the drug enrichment rate was fast and reached saturation after two days. The bioconcentration factor of TML in Crucian carp was 3.01. However, the drug had a slow elimination rate, with its complete metabolism occurring after 20 days.

Establishment of withdrawal time and analysis of tiamulin residue in tissues of orally dosed broiler chickens

This study was conducted to investigate tiamulin (TML) residues in the edible tissues of orally dosed broiler chickens and to re-establish the withdrawal time (WT). Thirty-six healthy Ross broiler chickens were administered 0.5 (TML-1) and 2.5 kg (TML-2) per ton feed, respectively, of the drug containing TML 78 g/kg for 10 days. Twenty-four tissue samples were collected from 6 chickens in each of the TML-1 and TML-2 groups on 0, 1, 3, and 5 days after drug administration, respectively. The residual concentrations of TML were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The correlation coefficient of the calibration curves was 0.9978 to 0.9998, and the limits of detection and the limits of quantification (LOQ) were in the range of 0.03 to 0.06, and 0.1 to 0.2 µg/kg, respectively. Recoveries ranged between 89.0% to 116.7%, and the coefficients of variation were less than 13.9%. After the drug administration, TML in the TML-1 and TML-2 groups was detected above the LOQ in 1 and 6 samples of liver, respectively, at day 0, and in 1 liver sample from both groups on day one. At 3 days after administration, TML was detected below the LOQ in all samples of TML-1 and TML-2. The calculated WT of TML in both TML-1 and TML-2 using the WT calculation program WT 1.4 was 0 days. In conclusion, the developed analytical method is suitable for detection, and the calculated WT of TML in poultry edible tissues is shorter than the current recommended WT of 7 days for TML in broiler chickens.

Accelerated dissipation, soil microbial toxicity and dispersal of antimicrobial resistance in soils repeatedly exposed to tiamulin, tilmicosin and sulfamethoxazole

The application of manures leads to the contamination of agricultural soils with veterinary antibiotics (VAs). These might exert toxicity on the soil microbiota and threaten environmental quality, and public health. We obtained mechanistic insights about the impact of three VAs, namely, sulfamethoxazole (SMX), tiamulin (TIA) and tilmicosin (TLM), on the abundance of key soil microbial groups, antibiotic resistance genes (ARGs) and class I integron integrases (intl1). In a microcosm study, we repeatedly treated two soils (differing in pH and VA dissipation capacity) with the studied VAs, either directly or via fortified manure. This application scheme resulted in accelerated dissipation of TIA, but not of SMX, and accumulation of TLM. Potential nitrification rates (PNR), and the abundance of ammonia-oxidizing microorganism (AOM) were reduced by SMX and TIA, but not by TLM. VAs strongly impacted the total prokaryotic and AOM communities, whereas manure addition was the main determinant of the fungal and protist communities. SMX stimulated sulfonamide resistance, while manure stimulated ARGs and horizontal gene transfer. Correlations identified opportunistic pathogens like Clostridia, Burkholderia-Caballeronia-Paraburkholderia, and Nocardioides as potential ARG reservoirs in soil. Our results provide unprecedented evidence about the effects of understudied VAs on soil microbiota and highlight risks posed by VA-contaminated manures. Environmental implicationThe dispersal of veterinary antibiotics (VAs) through soil manuring enhances antimicrobial resistance (AMR) development and poses a threat to the environment and the public health. We provide insights about the impact of selected VAs on their: (i) microbially-mediated dissipation in soil; (ii) ecotoxicity on the soil microbial communities; (iii) capacity to stimulate AMR. Our results (i) demonstrate the effects of VAs and their application-mode on the bacterial, fungal, and protistan communities, and on the soil ammonia oxidizers; (ii) describe natural attenuation processes against VA dispersal, (iii) depict potential soil microbial AMR reservoirs, essential for the development of risk assessment strategies.

Degradation of tiamulin by a packed bed dielectric barrier plasma combined with TiO2 catalyst

Recently, a plasma catalyst was employed to efficiently degrade antibiotic residues in the environment. In this study, the plasma generated in a packed bed dielectric barrier reactor combined with TiO2 catalyst is used to degrade the antibiotic tiamulin (TIA) loaded on the surface of simulated soil particles. The effects of applied voltage, composition of the working gas, gas flow rate and presence or absence of catalyst on the degradation effect were studied. It was found that plasma and catalyst can produce a synergistic effect under optimal conditions (applied voltage 25 kV, oxygen ratio 1%, gas flow rate 0.6 l min−1, treatment time 5 min). The degradation efficiency of the plasma combined with catalyst can reach 78.6%, which is 18.4% higher than that of plasma without catalyst. When the applied voltage is 30 kV, the gas flow rate is 1 l min−1, the oxygen ratio is 1% and the plasma combined with TiO2 catalyst treats the sample for 5 min the degradation efficiency of TIA reached 97%. It can be concluded that a higher applied voltage and longer processing times not only lead to more degradation but also result in a lower energy efficiency. Decreasing the oxygen ratio and gas flow rate could improve the degradation efficiency. The relative distribution and identity of the major TIA degradation product generated was determined by high-performance liquid chromatography–mass spectrometry analysis. The mechanism of TIA removal by plasma and TiO2 catalyst was analyzed, and the possible degradation path is discussed.

UHPLC-MS/MS Analysis of Antibiotics Transfer and Concentrations in Porcine Oral Fluid after Intramuscular Application.

The monitoring of antibiotic use in animals is a crucial element to ensure food safety. The main goal of this study was to analyse the distribution of selected antibiotics to porcine oral fluid, as well as to demonstrate that an oral fluid is an alternative to other biological matrices used in the control of antibacterials. Therefore, an animal study with pigs treated using seven different antibiotics was performed. Sulfadoxine (SDX) with trimethoprim (TRMP), lincomycin (LIN), tiamulin (TIAM), tylosin (TYL), amoxicillin (AMX) and penicillin G (PEN G) were injected intramuscularly to pigs, and concentrations of these analytes in the oral fluid were assessed. Ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS) was used to quantify the analytes. On the first day of medication, the highest concentrations for SDX and TRMP at the level of 22,300 µg/kg and 14,100 µg/kg were found, respectively. The concentrations of LIN (10,500 µg/kg) and TIAM (7600 µg/kg) were also relatively high. The peak of TYL was recorded on the second day of drug administration. Most of the analytes were present in oral fluid for 30 days, apart from TYL, which was detected for 27 days. It was found that AMX and PEN G were quantified only for 5 and 8 days, respectively, at very low concentrations. It was found that oral fluid can be used for the verification of antibiotics on pig farms.

Following the route of veterinary antibiotics tiamulin and tilmicosin from livestock farms to agricultural soils

Veterinary antibiotics (VAs) are not completely metabolized in the animal body. Hence, when animal excretes are used as soil manures, VA residues are dispersed with potential implications for environmental quality and human health. We studied the persistence of tiamulin (TIA) and tilmicosin (TLM) along their route from pig administration to fecal excretion and to agricultural soils. TLM was detected in feces at levels folds higher (4.27–749.6 μg g-1) than TIA (0.55–5.99 μg g-1). Different administration regimes (feed or water) showed different excretion patterns and residual levels for TIA and TLM, respectively. TIA and TLM (0.5, 5 and 50 μg g-1) dissipated gradually from feces when stored at ambient conditions (DT50 5.85–35.9 and 23.5–49.8 days respectively), while they persisted longer during anaerobic digestion (DT90 >365 days) with biomethanation being adversely affected at VA levels > 5 μg g-1. When applied directly in soils, TLM was more persistent than TIA with soil fumigation extending their persistence suggesting microbial degradation, while soil application through feces increased their persistence, probably due to increased sorption to the fecal organic matter. The use of TIA- and TLM-contaminated feces as manures is expected to lead to VAs dispersal with unexplored consequences for the environment and human health.

Development of a label free electrochemical sensor based on a sensitive monoclonal antibody for the detection of tiamulin

Based on a murine monoclonal antibody (mAb) against tiamulin (TML), an electrochemical immunosensor was proposed using silver-graphene oxide (Ag-GO) nanocomposites and gold nanocomposites (AuNPs) to detect tiamulin (TML). Due to the synergetic properties of Ag-GO nanocomposites and AuNPs, the conductivity of the immunosensor was significantly enhanced. On account of the specific mAb and conductive nanocomposites, the proposed electrochemical immunosensor exhibited a low LOD of 0.003 ng mL−1 for the detection of TML in a wide linear range of 0.01 to 1000 ng mL−1. In addition, the immunosensor did not involve additional redox species. Furthermore, the efficient and simple electrochemical immunosensor was employed to detect TML in real samples with high accuracy, suggesting a potential detection platform for other veterinary antibiotics in animal derived foods.

A novel electrochemical immunosensor for the sensitive detection of tiamulin based on staphylococcal protein A and silver nanoparticle-graphene oxide nanocomposites

Tiamulin (TML) is a pleuromutilin antibiotic and mainly used to treat pulmonary and gastrointestinal infections. However, excessive use of TML can bring health threats to consumers. In this work, a label-free electrochemical immunosensor was proposed for sensitive detection of TML in pork and pork liver. Silver nanoparticles (AgNPs) were synthesized in situ on graphene oxide (GO), in which GO acted as a carrier for loading more AgNPs and AgNPs exhibited both strong conductivity and good redox property. In addition, staphylococcal protein A (SPA) was applied to oriented immobilization of fragment crystallizable (Fc) region of the TML monoclonal antibody. Under the optimal condition, the developed electrochemical immunosensor exhibited a good linear response with a concentration of TML ranging from 0.05 ng mL−1 to 100 ng mL−1 and the limit of detection (LOD) was 0.04 ng mL−1. Furthermore, the designed immunosensor was applied to detect TML in real samples with a good accuracy. Therefore, the label-free electrochemical immunosensor could be used as a potential method to detect TML and other antibiotic residues in animal derived foods.

Characterization of a Novel Gene, srpA, Conferring Resistance to Streptogramin A, Pleuromutilins, and Lincosamides in Streptococcus suis

Antimicrobial resistance is undoubtedly one of the greatest global health threats. The emergence of multidrug-resistant (MDR) Gram-positive pathogens, such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), and β-lactamase-resistant Streptococcus pneumonia, has severely limited our antibiotic arsenal. Numerous ribosome-targeting antibiotics, especially pleuromutilins, oxazolidinones, and streptogramins, are viewed as promising alternatives against aggressive MDR pathogens. In this study, we identified a new adenosine triphosphate (ATP)-binding cassete (ABC)-F family determinant, srpA, in Streptococcus suis (S. suis) by means of a comparative analysis of the whole-genome sequences of tiamulin (TIA)-resistant and TIA-sensitive bacteria. Functional cloning confirmed that the deduced gene can mediate cross-resistance to pleuromutilins, lincosamides, and streptogramin A in S. suis and S. aureus. A sequence alignment revealed that SrpA shares the highest amino acid identity with Vga(E) (36%) and shows canonical characteristics of ABC-F family members. In SrpA-ribosome docked compounds, the extended loop region of SrpA approaches the valnemulin-binding pocket in the ribosome peptidyl-transferase center and competes with bound valnemulin. A detailed mutational analysis of the loop residues confirmed that this domain is crucial for SrpA activity, as substitutions or truncations of this region affect the efficiency and specificity of antibiotic resistance. Intracellular antibiotics accumulation indicated that SrpA does not act as an efflux pump, while a ribosome binding assay supported the protective effects of SrpA on the ribosome by preventing antibiotic binding as well as displacing bound drugs. These findings clarify the mechanisms underlying resistance to ribosomal antibiotics.

Effect of sludge recirculation on removal of antibiotics in two-stage membrane bioreactor (MBR) treating livestock wastewater.

Two-stage MBR consisting of anaerobic and aerobic reactors was operated at total hydraulic retention time (HRT) of 48h for the treatment of livestock wastewater containing antibiotics, i.e. amoxicillin (AMX), tiamulin (TIA), and chlortetracycline (CTC), under the (1st) absence and (2nd) presence of sludge recirculation between the reactors. During the operation with sludge recirculation, the removals of organic and nitrogen were enhanced. Meanwhile, the removals of TIA and CTC were found to decrease by 9% and 20% in the aerobic reactor but increased by 5% to 7% in the anaerobic reactor due to the relocation of biomass from the aerobic to the anaerobic reactor. A high degree of AMX biodegradation under both anaerobic and aerobic conditions and partial biodegradation of TIA and CTC under aerobic conditions were confirmed in batch experiments. Moreover, the effect of sludge recirculation on biomass and pollutant removal efficiencies in the 2-stage MBR was revealed using microbial community analyses. Membrane filtration also helped to retain the adsorbed antibiotics associated with small colloidal particles in the system.

Metabolitos secundarios con actividad medicinal extraídos de hongos provenientes de Centroamérica

Existe una gran diversidad de hongos que poseen actividad medicinal en Centroamérica y de ellos se pueden extraer los metabolitos secundarios responsables y adecuados para el tratamiento de distintas enfermedades. En esta revisión se realizó una investigación acerca de seis hongos específicos que se utilizan para el tratamiento de padecimientos físicos: Penicillium chrysogenum (P. chrysogenum), Ganoderma lucidum (G. lucidum), Trametes versicolor (T. versicolor), Schizophyllum commune (S. commune), Pleurotus mutilus (P. mutilus), Lentinula edodes (L. edodes), y un último hongo que tiene propiedades alucinógenas útiles en el tratamiento de enfermedades mentales: Psilocybe cubensis (P. cubensis). Se encontró que los metabolitos secundarios de mayor importancia para la industria médica son la penicilina y la tiamulina como antibióticos, los triterpenos por su actividad anticancerígena, diversos tipos de polisacáridos que tienen actividad antioxidante, antiparasitarios, estimulantes del sistema inmune y reguladores del colesterol. Asimismo, se estudió el alucinógeno psilocibina y su potencial en tratamientos de psicoterapia.

Rapid liquid chromatographic method for the control of doxycycline and tiamulin residues and their metabolites in vivo assays with pigs: Treatment and depletion

Results gained from in vivo metabolism could be more authentic to reflect the biotransformation of drugs than in vitro metabolism. This paper discusses the development and characterization of an analytical method for controlling tiamulin (TIA) and doxycycline (DOX) residues in pig plasma and urine, as well as their in vivo biotransformation during treatment and suppression steps at pig farms. For such a purpose, urine samples were taken daily (24-hr intervals) during treatment and until day 8 after last dose. Plasma samples were collected on the last treatment day and up to days 4 and 7 after the last dose. Only traces of TIA and their metabolites have been still detected 8 days after the last day of therapy, in agreement with the period of suppression of both antibiotics set by the manufacturers in 7 days. It was estimated that TIA and DOX half-lives were of two days and one day, respectively. TIA and DOX metabolites have been identified for the first time in plasma and urine pig samples during treatment and suppression steps.

Bacterial community structures and biodegradation kinetic of Tiamulin antibiotic degrading enriched consortia from swine wastewater

The antibiotic tiamulin (TIA) is common and widely used medication for dysentery eradication in swine productions. Tiamulin persists in livestock manure, and its residues have been found in various environment. This work obtained four tiamulin-degrading enriched bacterial consortia from a covered anaerobic lagoon system and a stabilized pond system of swine farms. Tiamulin was efficiently removed by the enriched cultures at the concentrations between 2.5 and 200mg/L, with a removal of 60.1-99.9% during 16h and a degradation half-life of 4.5-15.7h. The stabilized pond system cultured with taimulin solely could eliminate tiamulin at the highest rates. The logistic substrate degradation model fit most of the experimental data. Next-generation amplicon sequencing was conducted, and it was found that the bacterial community was significantly impacted by the inoculum source, nutrient addition, and high tiamulin concentrations. Principal coordinate analysis (PCoA) indicated the similarity of bacterial communities in the original enriched samples and the 2.5mg/L tiamulin-removed cultures. The 200mg/L consortia were rather different and became similar to the other 200mg/L consortia from different sources and cultures without nutrient supplementation. Shannon and Simpson indices suggested a reduction in bacterial diversity at high concentrations. The microbes that had high growth in the most efficient enriched culture, or which were abundant in all samples, or which increased with higher tiamulin concentrations were likely to be the major tiamulin-degrading bacteria. This is the first report suggested the possible roles of Achromobacter, Delftia, Flavobacterium, Pseudomonas, and Stenotrophomonas in tiamulin degradation.

Development of Indirect Competitive Enzyme-Linked Immunosorbent and Immunochromatographic Strip Assays for Tiamulin Detection in Chicken.

Tiamulin (TML) is a diterpenoid antibiotic used in animals. In this study, a gold nanoparticle immunochromatographic strip assay and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect the residue of TML in chicken. TML aminobutyric was synthesized and conjugated to keyhole limpet hemocyanin by mixed anhydride method as immunogen, whereas TML was connected to ovalbumin with 1,1′-carbonyldiimidazole as coating antigen. Under optimized conditions, the ultrasensitive monoclonal antibody-based ic-ELISA exhibited a half-maximal inhibitory concentration (IC50) value of 0.36 ng/mL with a working range of 0.14–0.9 ng/mL for TML. A rapid and sensitive immunochromatographic strip assay was developed with a TML cutoff value of 2.5 ng/mL. On the basis of these results, both developed methods are useful for TML detection in chicken.

Tiamulin removal by wood-rot fungi isolated from swine farms and role of ligninolytic enzymes

Tiamulin (TIA) is a widely used antibiotic in Thai swine farms and persists in swine manure storage. Significant amounts remain in swine manure after composting, posing risks related to the spread of the compound and the evolution of resistant genes when this compost is used as a fertilizer. Therefore, it is necessary to enhance TIA degradation. This study successfully purified TIA-degrading fungi, which have a high capacity for treating antibiotic-contaminated manure. Twelve strains were collected from swine farms and preliminarily examined for their ability to degrade recalcitrant compounds using brilliant green and crystal violet dyes at 0.05% (w/v). Three isolated fungi and three white-rot fungal strains–namely, Lasiodiplodia sp. F1, Fusarium sp. F5, Galactomyces sp. F8, Verticillium sp., Trametes versicolor, and Trametes hirsuta AK4–capable of developing considerable decolorized zones on both dyes exhibited their abilities to degrade 10 mg l−1 of TIA over a period of 12 days: 93.2 ± 0.0%, 82.4 ± 10.2%, 73.8 ± 5.6%, 89.3 ± 0.0%, 86.1 ± 0.0%, and 66.8 ± 4.8%, respectively. Lasiodiplodia sp. F1, Verticillium sp., and Trametes versicolor were able to remove TIA most efficiently (>85%) and have the potential to be applied to swine waste for the elimination of antibiotic substances. Manganese peroxidase (MnP) was predominantly produced; MnP expression was induced by tiamulin, suggesting its main role in TIA degradation.

Effects of amoxicillin, ceftiofur, doxycycline, tiamulin and tulathromycin on pig humoral immune responses induced by erysipelas vaccination

It addition to their antimicrobial properties, antibiotics can influence the host immune system (modulation of cytokine secretion, antibody production and T-cell proliferation). In the present study, the authors studied the...