Thyroid T4 Research Articles

The role of anti-TPO as an additional analytical marker in thyroid disease patients in Erbil City

Background: There is a connection among anti-TPO and anti-TG antibodies and levels of thyroid hormone and both alone or in grouping have been utilized to expect perfection of hypo or hyperthyroidism.
 Objective: Both alone or in grouping have been utilized to expect perfection of hypo or hyperthyroidism.
 Patients and Methods: This is a cross-sectional study achieved in the laboratories of Rizgary Teaching Hospital and Erbil Teaching Hospital in Erbil, Iraq, within the period of May 2020 to April 2021. The study included 66 patients, both males and females included. These patients had clinical indications and were suspected of having a sort of thyroiditis. Anti-TPO electrochemiluminescence immunoassay analysis has been included by the analysts in conjunction with conventional markers immunoassay of thyroid TSH, T3, and Free T4 trusting that this would help in lessening morbidity and related wellbeing concerns. 
 Results: There was an increase in the level of anti-TSH antibodies in the hyperthyroidism group (60.6%) which was more than in those with hypothyroidism (36.4%), significant association exist (P≤0.049). Moreover, the level of Anti-TPO Abs was higher among hypothyroidism patients (63.6%) than among hyperthyroidism cases (33.3%), this association was statistically significant (P≤0.014).
 Conclusion: Demonstrating the clinical importance of this antibody and the benefit of adding anti-TPO, in combination with TSH and FT4. Addition of one test could potentially save expenditure on long-term diseases such as overt thyroid disease and its attended morbidities.

Review article on endocrine diseases during pregnancy

Pregnancy is the period where a woman undergoes several physical and hormonal changes in accordance to the growth of the fetus. Wide alteration in the levels of hormones from the normal values affect the state of gestation and the growth of the baby. Especially the most commonly encountered disorders like pregnancy induced thyroid disorder effects the neuro development of the baby whereas gestational diabetes makes mode of delivery complex. Hormonal imbalance can be investigated by haematological findings like thyroid stimulating hormone (TSH), T3 and T4 tests to identify thyroid disorders whereas HbA1C, Random blood sugar(RBS), fasting random blood sugar(FRBS) , post prandial blood sugar (PPBS) and oral glucose tolerance test (OGTT) to screen gestational diabetes. Apart form these there are some uncommon hormonal pregnancy disorders like cushing syndrome, conns syndrome, congenital adrenal hyperplasia, adrenal insufficiency. These complexes can be rectified up to certain extent by diagnosing them before hand thereby implementing life style changes, dietary changes and taking medications. Therefore, prior screening of the diseases is important in supress further complications in pregnancy and to have healthy progeny.

Sustained Elimination of Iodine Deficiency Within the Third Decade After Compulsory Iodine Supplementation Policy in the South of Iran: A Population-Based Cross-Sectional Study

ObjectivesRecently, some studies in Iran have shown mild to moderate iodine concentrations in adult and pregnant women populations despite iodine sufficiency in children. This study aimed to evaluate the urine iodine status and salt intake among adult households in the city of Sadra, Fars province in southern Iran, and to assess its possible influencing factors. MethodParticipant households for this cross-sectional study were selected using randomized cluster sampling in the city of Sadra, Fars province, southern Iran from 1 February, 2021 to 30 November, 2021. Two subjects >18 y of age from each household were invited. Ninety-two subjects (24 men, 68 women) were enrolled. The participants were asked to collect their 24 h urine. They were then examined for thyroid disorders and subjected to thyroid ultrasonography and thyroid function tests. Urine samples were tested for iodine, sodium, and creatinine concentrations. Household salt intake was also estimated. ResultsMedian urine iodine content (UIC) in the participants was 175 (IQR: 117, 250) μg/L, whereas the median salt consumption per person per day was 9.6 (IQR, 7.3-14.5) g. Sex, methods of salt storage, presence of goiter or thyroid nodules, the addition of salt in the cooking stage, and subclinical hypothyroidism had no effect on UIC, whereas individuals with hypertension and lower education had significantly lower iodine concentrations. UIC had a significant positive correlation with urine sodium and thyroid stimulating hormones (TSH) concentrations (P < 0.001, 0.046) and a negative correlation with thyroid volume and T4 (P = 0.029, 0.018). ConclusionIodine status in the adult population of Sadra city was categorized as sufficient, although the iodine concentrations reported in Tehran were insufficient. The contributing factor can be higher salt consumption or possible higher environmental iodine concentrations in Sadra city than Tehran.

COVID-19 and thyroid function: What do we know so far?

Coronavirus disease 2019 (COVID-19) was characterized as a pandemic in March, 2020 by the World Health Organization. COVID-19 is a respiratory syndrome that can progress to acute respiratory distress syndrome, multiorgan dysfunction, and eventually death. Despite being considered a respiratory disease, it is known that other organs and systems can be affected in COVID-19, including the thyroid gland. Thyroid gland, as well as hypothalamus and pituitary, which regulate the functioning of most endocrine glands, express angiotensin-converting enzyme 2 (ACE2), the main protein that functions as a receptor to which SARS-CoV-2 binds to enter host cells. In addition, thyroid gland is extremely sensitive to changes in body homeostasis and metabolism. Immune system cells are targets for thyroid hormones and T3 and T4 modulate specific immune responses, including cell-mediated immunity, natural killer cell activity, the antiviral action of interferon (IFN) and proliferation of T- and B-lymphocytes. However, studies show that patients with controlled hypothyroidism and hyperthyroidism do not have a higher prevalence of COVID-19, nor do they have a worse prognosis when infected with the virus. On the other hand, retrospective observational studies, prospective studies, and case reports published in the last two years reported abnormal thyroid function related to acute SARS-CoV-2 infection or even several weeks after its resolution. Indeed, a variety of thyroid disorders have been documented in COVID-19 patients, including non-thyroidal illness syndrome (NTIS), subacute thyroiditis and thyrotoxicosis. In addition, thyroid disease has already been reported as a consequence of the administration of vaccines against SARS-CoV-2. Overall, the data revealed that abnormal thyroid function may occur during and in the convalescence post-COVID condition phase. Although the cellular and molecular mechanisms are not completely understood, the evidence suggests that the "cytokine storm" is an important mediator in this context. Thus, future studies are needed to better investigate the pathophysiology of thyroid dysfunction induced by COVID-19 at both molecular and clinical levels.

Performance and health of broiler chickens fed low erucic acid, lower fiber pennycress (CoverCressTM) grain

CoverCress (low erucic acid, lower fiber pennycress) is being developed as a cover crop to be planted in the fall after corn and harvested in the spring prior to planting soybeans. Two experiments were conducted to evaluate 2 lines of the whole grain (CCWG-1: natural mutation and mutation breeding; CCWG-2: gene edited) and the whole grain pretreated with the potential palatability agent copper sulfate (CCWG-1-CuSO4; CCWG-2-CuSO4) as an ingredient for broilers. In Experiment 1, CCWG-1-CuSO4 was included in the diet at 0, 4, and 6% for 41 d. Feed intake, body weight gain, feed conversion, processing characteristics, organ weights, serum thyroid, macropathology and histology data were collected. In Experiment 2, broilers were fed diets containing Control, 2% CCWG-1, 4% CCWG-1, 4% CCWG-2, and 4.35% CCWG-1-CuSO4 for 42 d. Feed intake, body weight gain, feed conversion, organ weights, serum thyroid, blood chemistries, macropathology, and histology data were collected. In Experiment 1, feed intake and body weight were diminished with no effect on feed conversion for the birds consuming diets containing CCWG-1-CuSO4. In Experiment 2, feed intake and body weight were lower with no difference in feed conversion in birds fed diets containing greater than 2% CoverCress grain during d 0 to 28. During d 28 to 42 no difference in feed intake, body weight and an improvement in feed conversion was observed in birds fed all of the CoverCress grain products. In both experiments no significant negative effects were observed in processing, liver, kidney, and thyroid weights, T3, T4, blood chemistries, macropathology, and histopathology between the control and any of the CoverCress grain treatments. No difference in performance was observed in birds fed the mutant (4% CCWG-1) and gene-edited (4% CCWG-2) products. Pretreating CoverCress grain with copper sulfate did not have a significant effect on improving palatability. In conclusion, CoverCress grain can be safely fed to broilers when included at a target rate of 4% in diets and with total glucosinolate levels not to exceed 4.9 µmoles g-1.

Mechanisms and Management of Thyroid Disease and Atrial Fibrillation: Impact of Atrial Electrical Remodeling and Cardiac Fibrosis.

Atrial fibrillation (AF) is the most common cardiac arrhythmia associated with increased cardiovascular morbidity and mortality. The pathophysiology of AF is characterized by electrical and structural remodeling occurring in the atrial myocardium. As a source of production of various hormones such as angiotensin-2, calcitonin, and atrial natriuretic peptide, the atria are a target for endocrine regulation. Studies have shown that disorders associated with endocrine dysregulation are potential underlying causes of AF. The thyroid gland is an endocrine organ that secretes three hormones: triiodothyronine (T3), thyroxine (T4) and calcitonin. Thyroid dysregulation affects the cardiovascular system. Although there is a well-established relationship between thyroid disease (especially hyperthyroidism) and AF, the underlying biochemical mechanisms leading to atrial fibrosis and atrial arrhythmias are poorly understood in thyrotoxicosis. Various animal models and cellular studies demonstrated that thyroid hormones are involved in promoting AF substrate. This review explores the recent clinical and experimental evidence of the association between thyroid disease and AF. We highlight the current knowledge on the potential mechanisms underlying the pathophysiological impact of thyroid hormones T3 and T4 dysregulation, in the development of the atrial arrhythmogenic substrate. Finally, we review the available therapeutic strategies to treat AF in the context of thyroid disease.

Can Thyrotropin, Tri-Iodothyronine, and Thyroxine Hormones be Predictors of Cancer in Thyroid Lesions?

Background Thyroid nodules are a common medical problem worldwide. This study aims to investigate and elucidate the relationship between thyroid-stimulating hormone (TSH), thyroxine (T4), and triiodothyronine (T3), and malignant thyroid nodules. Methods This prospective cross-sectional study was conducted at a public specialist hospital in Saudi Arabia from February 2020 to February 2021. All thyroid nodules were scanned using ultrasound imaging, and the largest diameter was measured for each and classified according to the American College of Radiology (ACR) Thyroid Imaging Reporting and Data System (TIRADS) classification system. Thyroid function tests TSH, T3, and T4 were measured. Definitive diagnoses of thyroid nodules were given based on cytology. A one-way analysis of variance (ANOVA) test was used to compare means, and cross-tabulation was used to correlate the variables in the study. Results A total of 222 patients participated in this study; 23.42% were male and 76.57% were females. The mean age was 44.73 ± 13.31 years (range: 18 to 85 years). The percentage of malignancy was 20.6%, 36.3%, and 91.2% in TIRADS 3, TIRADS 4, and TIRADS 5, respectively. A weak positive linear relationship was noted between nodule size and TSH (R2= 0.012). The study demonstrates that TSH increases in malignant nodules more than in benign nodules, while T4 and T3 are decreased in malignant nodules. Conclusion The level of TSH increases in patients with malignant thyroid nodules more than in benign nodules, which can be used as a predictor of malignancy, while T4 and T3 reduced in malignant nodules with an ambiguous relationship.

Studying the Association Between Systolic Blood Pressure and Thyroid Stimulating Hormone in Newly Diagnosed Subclinical Hyperthyroidism Female Patients

Background: Subclinical hyperthyroidism is a condition in which thyroid stimulating hormone (TSH) is mildly decreased with both free iodine thyroxine (free T4) and triiodothyronine (T3) within the normal range. The aim of this research is to determine the association between TSH with lipid profiles, age, blood pressure and body mass index (BMI) and also to evaluate lipid profiles in subclinical hyperthyroid patients. Materials and Methods: Ninety patients with subclinical hyperthyroidism were chosen as the patient group and 50 healthy individuals age-matched were chosen as the control group. Thyroid function tests TSH, T4 and T3 were performed by VIDAS and on the same day lipid profiles were performed by (Biolis 24i Premium) in the laboratory of General Zakho Hospital, Kurdistan Region, Iraq. Results: In subclinical hyperthyroidism the BMI and diastolic blood pressure values were non-significantly low (P=0.13 and p=0.27 respectively) whereas systolic blood pressure increased slightly but was still non-significant. TSH was significantly and negatively related to systolic blood pressure. Conclusion: Lipid profiles and systolic and diastolic blood pressure are not altered in subclinical hyperthyroidism. However, systolic blood pressure is related to the severity of the decrease in TSH levels.

ODP513 Rhabdomyolysis: A Rare Presentation Of Central hypothyroidism

Abstract Background Hypothyroidism is one of the most common endocrine disorders affecting approximately 5 percent of Americans aged 12 and older. The most common cause of hypothyroidism globally is iodine deficiency whereas in the United States, Hashimoto's thyroiditis is the most prevalent. Less frequently, hypothyroidism can be caused by central hypothyroidism. In uncontrolled states, this entity can lead to an array of complications with rhabdomyolysis being among the latest manifestations. We present a case when the patient was admitted to the hospital for significant rhabdomyolysis, and after workup, was found to be related to new onset central hypothyroidism. Clinical Case A 34-year-old African American male presented to our facility with proximal muscle weakness and pain in the bilateral hips and right shoulder for three days. During the interview, the patient was acutely debilitated demonstrating slow body movements and grimaces on passive and active range of motion. Motor strength and sensation was preserved in bilateral extremities. The patient denied any recent injury, compression, extensive exercise, seizure, or durg use. The patient, however, reported a family history of rheumatological and endocrine conditions. Labs were obtained and revealed a creatinine kinase level of 10,5000 U/L; creatinine kinase- MB 53.5 ng/mL; creatinine 1. 0 mg/dL; AST 146 U/L; ALT 72 U/L; CRP 8.6 mg/L. The patient was stabilized with appropriate fluid and steroid therapy. On further investigation, TSH and T4 levels were 0.30 µlU/mL and 0.55 ng/dL, respectively. MRI with and without contrast did not show any intracranial pituitary abnormality or mass. The patient was started on levothyroxine and instructed to remain on the drug as an outpatient to prevent future occurrences. He was subsequently discharged after CK levels normalized and proximal muscle weakness improved. The patient was instructed to have a repeat thyroid function test and T4 levels in 6 weeks with appropriate follow up. Conclusion Idiopathic central hypothyroidism is a rare cause of hypothyroidism estimated to occur in 1: 20,000-80,000 in the general public. The lack of a pituitary mass lesion on MRI added to the rarity of this presentation. Hypothyroid myopathy causing rhabdomyolysis is a known but rare consequence of untreated hypothyroidism, which may present with muscle weakness, pain, and elevated serum muscle enzyme. Prompt workup to rule out a multitude of other causes is required, and the patient should be closely monitored for electrolyte and kidney function changes. Levothyroxine remains the treatment of choice for correction of central hypothyroidism with dose adjustments based on patient symptoms and serum free T4 values. Presentation: No date and time listed

Prevalence of Thyroid Problems Among Patients Attending at Siddharthanagar City Hospital

Thyroid gland is vital gland. It produces Thyroxine T3 and Tri-Idothyronine T4 hormones which are needed for the growth and development. Iodine is needed for the formation of T3 and T4 hormones. Iodine is found in different food such as sea food, vegetables produced in Iodine rich soil and table salt. The associated problems of thyroid gland are hypothyroidism and hyperthyroidism. The objective of this study was to find out the prevalence of thyroid problems attending at Siddharthanagar City Hospital.&#x0D; It was hospital based retrospective study. Data related to thyroid problems T3,T4 and TSH was collected from hospital laboratory reports . The tests were done using Semi Auto Clia Plate Analyzer of TOSOH India Company.&#x0D; The total number of lab reports was 299 taken for study. Among them the percentage of male and female was 21.7 and 78.3 respectively. The overall prevalence of thyroid problems was 5.9% among general public. The overall prevalence of hypothyroidism was 9.69% and hyperthyroidism was 2.17% respectively. The prevalence of thyroid problems was seen more in female.&#x0D; This study showed that thyroid problem including hyperthyroidism and hypothyroidism is common. Female population and 21-40 years age group are highly suffered in comparison to male population.

Thyrotoxicosis complicating a COVID-19 infection

The SARS-COV-2 infection, in the typical case, results in acute forms of pneumonia, the severe forms of which can be life-threatening. However, it can also cause various extrapulmonary attacks (cutaneous, neurological, cardiovascular, ophthalmological, etc.) but also endocrine (pancreatic, pituitary, etc.), and in particular thyroid disorders associated with COVID-19. In this case report, we display the unusual case of a thyrotoxicosis due to the COVID-19 in a woman of 57 years of age with a severe pneumonia, which appeared on the ninth day of her infection. The thyrotoxicosis was suspected in front of a sudden tachycardia with no apparent cause, associated with a swollen neck. The diagnosis is confirmed by an elevation of thyroid enzymes T4 and T3, with a thyroid ultrasound revealing multiple hypoechoic area. The patient was treated with steroids and the symptoms have gradually lessened. In conclusion, subacute thyroiditis secondary to SARS-COV-2 infection is a hitherto poorly described complication that may go unnoticed because of its delayed onset. A clinical examination for signs of thyrotoxicosis and pain in the anterior neck region is usually sufficient to evoke this diagnosis. Thyroid hormone assay may be useful to confirm and treat this entity

From hormone replacement therapy to regenerative scaffolds: A review of current and novel primary hypothyroidism therapeutics.

Primary hypothyroidism severely impacts the quality of life of patients through a decrease in the production of the thyroid hormones T3 and T4, leading to symptoms affecting cardiovascular, neurological, cognitive, and metabolic function. The incidence rate of primary hypothyroidism is expected to increase in the near future, partially due to increasing survival of patients that have undergone radiotherapy for head and neck cancer, which induces this disease in over half of those treated. The current standard of care encompasses thyroid hormone replacement therapy, traditionally in the form of synthetic T4. However, there is mounting evidence that this is unable to restore thyroid hormone signaling in all tissues due to often persistent symptoms. Additional complications are also present in the form of dosage difficulties, extensive drug interactions and poor patience compliance. The alternative therapeutic approach employed in the past is combination therapy, which consists of administration of both T3 and T4, either synthetic or in the form of desiccated thyroid extract. Here, issues are present regarding the lack of regulation concerning formulation and lack of data regarding safety and efficacy of these treatment methods. Tissue engineering and regenerative medicine have been applied in conjunction with each other to restore function of various tissues. Recently, these techniques have been adapted for thyroid tissue, primarily through the fabrication of regenerative scaffolds. Those currently under investigation are composed of either biopolymers or native decellularized extracellular matrix (dECM) in conjunction with either primary thyrocytes or stem cells which have undergone directed thyroid differentiation. Multiple of these scaffolds have successfully restored an athyroid phenotype in vivo. However, further work is needed until clinical translation can be achieved. This is proposed in the form of exploration and combination of materials used to fabricate these scaffolds, the addition of peptides which can aid restoration of tissue homeostasis and additional in vivo experimentation providing data on safety and efficacy of these implants.

Extranuclear effects of thyroid hormones and analogs during development: An old mechanism with emerging roles

Thyroid hormones, T3 (triiodothyronine) and T4 (thyroxine), induce a variety of long-term effects on important physiological functions, ranging from development and growth to metabolism regulation, by interacting with specific nuclear or cytosolic receptors. Extranuclear or nongenomic effects of thyroid hormones are mediated by plasma membrane or cytoplasmic receptors, mainly by αvβ3 integrin, and are independent of protein synthesis. A wide variety of nongenomic effects have now been recognized to be elicited through the binding of thyroid hormones to this receptor, which is mainly involved in angiogenesis, as well as in cell cancer proliferation. Several signal transduction pathways are modulated by thyroid hormone binding to αvβ3 integrin: protein kinase C, protein kinase A, Src, or mitogen-activated kinases. Thyroid hormone-activated nongenomic effects are also involved in the regulation of Na+-dependent transport systems, such as glucose uptake, Na+/K+-ATPase, Na+/H+ exchanger, and amino acid transport System A. Of note, the modulation of these transport systems is cell-type and developmental stage-dependent. In particular, dysregulation of Na+/K+-ATPase activity is involved in several pathological situations, from viral infection to cancer. Therefore, this transport system represents a promising pharmacological tool in these pathologies.

Serum Selenium Levels in Patients With Graves Disease With or Without Thyroid Ophthalmopathy

BackgroundGraves disease is one of the most common autoimmune thyroid diseases. Thyroid has the highest concentration of selenium (Se) in the body. Se plays a crucial role in the functioning of some thyroid enzymes; however, there are controversial results regarding the administration of serum Se levels in patients with Graves disease. MethodsIn this study, patients with Graves disease with orbitopathy (GO group) or without orbitopathy (GD group) were recruited. Healthy individuals without a history of any disease were enrolled as the control group. Serum Se and thyroid hormone levels, including T3, T4, and thyroid-stimulating hormone (TSH), were measured using atomic absorption and radioimmunoassay techniques, respectively. ResultsIn this cross-sectional study, 60 and 56 patients and 58 healthy subjects were included in the GO, GD, and control groups. Serum Se levels in the GO, GD, and control groups were 94.53 ± 25.36 μg/dL, 96.82 ± 30.3 μg/dL, and 102.55 ± 16.53 μg/dL, respectively (P = .193). There was a reverse association between the serum Se level and thyroid hormones, including T3, T4, and TSH, in the GO group. However, serum Se levels exhibited a significant reverse association with T4 and TSH hormones but not with T3 in the GD group. ConclusionOur results showed no significant differences in the serum Se levels in the GO and GD groups compared with that in the control group. In addition, we did not detect any significant difference in the serum Se levels between the GO and GD groups.

Intrathyroidal feedforward and feedback network regulating thyroid hormone synthesis and secretion.

Thyroid hormones (THs), including T4 and T3, are produced and released by the thyroid gland under the stimulation of thyroid-stimulating hormone (TSH). The homeostasis of THs is regulated via the coordination of the hypothalamic-pituitary-thyroid axis, plasma binding proteins, and local metabolism in tissues. TH synthesis and secretion in the thyrocytes-containing thyroid follicles are exquisitely regulated by an elaborate molecular network comprising enzymes, transporters, signal transduction machineries, and transcription factors. In this article, we synthesized the relevant literature, organized and dissected the complex intrathyroidal regulatory network into structures amenable to functional interpretation and systems-level modeling. Multiple intertwined feedforward and feedback motifs were identified and described, centering around the transcriptional and posttranslational regulations involved in TH synthesis and secretion, including those underpinning the Wolff-Chaikoff and Plummer effects and thyroglobulin-mediated feedback regulation. A more thorough characterization of the intrathyroidal network from a systems biology perspective, including its topology, constituent network motifs, and nonlinear quantitative properties, can help us to better understand and predict the thyroidal dynamics in response to physiological signals, therapeutic interventions, and environmental disruptions.

Sex-specific effects of fluoride and lead on thyroid endocrine function in zebrafish (Danio rerio)

Fluoride (F) and lead (Pb) are widespread pollutants in the environment. F and Pb affect the thyroid endocrine system, but the mechanism of action between F and Pb is still unclear. In this study, in order to evaluate the effects of F or/and Pb on histopathological changes, antioxidant indices, the levels of thyroid hormones (THs), and the expression of endocrine-related genes in zebrafish thyroid. One thousand and two hundred zebrafish (female:male = 1:1) were randomly divided into four groups: control group (C group), 80 mg/L F group (F group), 60 mg/L Pb group (Pb group), and 80 mg/L F + 60 mg/L Pb group (F + Pb group) for 45 d and 90 d. Histopathological sections showed a loss of glia and follicular epithelial hyperplasia in the thyroid gland after exposure to F and Pb. Oxidative stress in the thyroid was induced after F and Pb exposure. And each oxidation index was increased after F + Pb exposure. Combined F and Pb exposure aggravated the downregulation of thyroid hormones T3 and T4 compared to exposure alone. Furthermore, F and Pb exposure altered the expression of thyroid endocrine-related genes in a time-dependent manner. These results indicate that F and Pb can affect the endocrine system of thyroid by changing the tissue structure, antioxidant capacity, thyroid hormone secretion and the levels of endocrine-related genes in thyroid. F and Pb can also produce toxic effects on thyroid, but the degree of poisoning is different in different indicators, mainly for the additive effect between them. Additionally, males are more sensitive than females to F or Pb toxicity. However, the specific molecular mechanism of the effects of F and Pb on thyroid endocrine system needs to be further studied.

Impact of ketogenic diet on insulin and thyroid hormones in a healthy cohort

Objective: To determine the effect of a ketogenic diet (KD) on the levels of insulin and thyroid hormone in healthy adult individuals. Methodology: This single-arm comparative experimental study was conducted at the Clinical trial room and Physiology laboratory, Khyber medial university, Peshawar. A total of 46 individuals were selected falling under the inclusion criteria. Ketogenic diet was given to the participants for four weeks. Blood was obtained from subjects on day 1, and after 29 days. Paired sample t test was used for pre- and post-trial comparison and ANOVA was used for comparison at day 0, and 29th day. Results: Mean age was 29.8±5.28 years. The mean value of thyroid stimulating hormone (0.005), T3 (&lt;0.001) and insulin (0.003) statistically decrease from day 1 to day 29. But mean value of T4 increased from day 1 (6.26±0.8301) to day 29 (7.70±0.931). However, there was no significant difference for thyroid releasing hormones (TRH) on day 1 and day 29 (0.139). Conclusion: The KD statistically reduced the level of TSH, T3, TRH and Insulin while T4 level increases. The clinicians should take into consideration patients with thyroid disorders or diabetes while prescribing KD.

Stem Cells from Healthy and Tendinopathic Human Tendons: Morphology, Collagen and Cytokines Expression and Their Response to T3 Thyroid Hormone.

The aim of this study was to investigate the effect of triiodothyronine (T3) on tendon specific markers and cytokines expression of stem cells extracted from human tendons. Indeed, thyroid hormones have been reported to be protective factors, maintaining tendons’ homeostasis, whereas tendinopathy is believed to be related to a failed healing response. Healthy and tendinopathic human tendons were harvested to isolate tendon stem/progenitor cells (TSPCs). TSPCs obtained from pathological samples showed gene expression and morphological modifications at baseline in comparison with cells harvested from healthy tissues. When cells were maintained in a medium supplemented with T3 (10−6 M), only pathological populations showed a significant upregulation of tenogenic markers (DCN, TNC, COL1A1, COL3A1). Immunostaining revealed that healthy cells constantly released type I collagen, typical of tendon matrix, whereas pathological ones overexpressed and secreted type III collagen, typical of scarred and impaired tissue. Pathological cells also overexpressed pro- and anti-inflammatory cytokines, suggesting an impaired balance in the presence of T3, without STAT3 activation. Moreover, DKK-1 was significantly high in the culture medium of pathological cell cultures and was reversed by T3. This study opens perspectives on the complex biochemical alteration of cells from pathological tendons, which may lead to the chronic disease context with an impaired extracellular matrix.

Responses to dietary supplementation with field bean (Vicia faba var. minor) in production indices, mohair growth and hormonal parameters in transition Angora goats

Twenty-two pluriparous single-foetus-bearing Angora goats, housed 3 weeks pre-partum in mid-December, and in transition from pregnancy to lactation were used to test the efficacy of post-partum supplementation (Group S: 11 goats) or not (Group C: 11 goats) with 300 g/head/day of whole field bean seeds (WFBS) to a diet based on mixed hay ad libitum and natural pasture. The goats were maintained with the suckling of their kids for 90 days. Significant decreases in liveweight (LW) and body condition score (BCS), were documented post-parturition in both groups and were then maintained throughout. These suggest a mobilisation in body tissue that was not mitigated by WFBS supplementation. However, the faster growth of the suckled kids and greater elongation of mohair fibre recorded (Group S > Group C), suggested improvement in partition towards production indices of lactation and hair fibre deposition, respectively. Greater concentrations of total thyroid hormones, T3 and T4 in supplemented does were recorded. Overall mean (pre-prandial) plasma insulin concentrations, not different between treatments, decreased (p < 0.5) from pre-partum concentration, and showed a pattern in time, similar to that of LW and BCS. Insulin appeared not to contribute to the suggested lactational and hair growth effects and possibly contributed to transition by a homeorhetic-type reduction in concentration post-partum. Mean plasma insulin-like growth factor-1 (IGF-1) concentrations trended lower due to supplementation from 3 weeks after kidding. It is concluded that WFBS supplementation was efficacious in improving production indices of the transition Angora goats. The feeding of different quantities of WFBS should be investigated. HIGHLIGHTS Supplementation of transition Angora goats with field bean did not affect the loss of body weight or body condition score occurring after kidding. Supplementation with field bean induced higher concentrations of blood thyroid hormones, without affecting post-partum reductions in insulin. Supplemented dams had a greater mohair elongation rate and their kids grew more, by likely preferential partition to hair growth and lactation, rather than other body tissues.

Comparing the values of anti-TPO and IL-17 among patients with thyroid disorders and comparing them with healthy controls in Ramadi City

Even though most thyroid subjects are undiagnosed due to nonspecific symptoms, universal screening for thyroid disease is not recommended for the general population. In this study, our motive is to compare thyroid autoantibody anti-TPO and IL-17 in addition to traditional thyroid markers TSH, T4, and T3 between two patients' groups (patients with hypothyroidism (TSH) &gt; 6 mIU/L and patients with hyperthyroidism TSH between 0.3 and 6 mIU/L) with a control group. Here a total of 45 subjects were divided into three groups, the first group consisted of 15 patients with hyperthyroidism. The second group consisted of 15 individuals with hypothyroidism, and the control group consisted of 15 healthy individuals. According to our results, an increase in TPO values was observed in all study groups, and the highest increase was recorded in the hyperthyroidism (386.672±116.924), and with regard to interleukin-17 values, a high value was observed in patients with hyperthyroidism (38.1033±5.44) compared to other groups.