Thyroid Cancer Research Research Articles

Molecular diagnostic tests in the diagnosis and management of thyroid carcinoma.

The application of molecular analysis of gene expression to clinical tissue samples represents one of the most exciting new areas in "translational" thyroid cancer research. Current data suggest that molecular diagnostic assays may improve the sensitivity and accuracy of fine needle aspiration of thyroid nodules, fine needle aspiration of metastases, and detection of recurrent disease in peripheral blood samples. It is likely that at least some of these tests will become useful adjuncts in the diagnostic armamentarium of clinical endocrinologists in the future.

Establishment of xenografts and cell lines from well‐differentiated human thyroid carcinoma

Background Our aim was to establish Chinese human thyroid cancer cell lines and to provide the material for thyroid cancer research. Methods We collected thyroid cancer tissues from 93 patients with thyroid cancer for developing the primary culture. Thyroid cancer tissues were verified by frozen sections during the operations, then subjected to primary cell culture. During the first several passages, fibroblasts were removed by selective attachment. Results From the 93 cancer tissues used, two follicular cancer tissues from the metastatic area (CGTH W-1, CGTH W-2) and one papillary thyroid cancer tissue (CGTH W-3) could be passed over 50 times. Cellular transformation with loss of contact inhibition occurred during passages 5–8. Electron microscopic studies of the CGTH W-1 cell line showed the presence of an abundance of mitochondria and Golgi complex. Presence of microvilli with interdigitations between neighboring cells were found in CGTH W-2 and CGTH W-3 cell lines. Severe combined immunodeficient (SCID) mice were used to determine whether these cells were tumorigenic. Two months after transplantation of 1 × 107 CGTH W-1, 2, and 3 cells into SCID mice, subcutaneous tumors ∼ 2–2.5 cm in size were clearly visible. After affinity cross-linking, an IGF-I with insulin-like growth factor binding proteins complex corresponding to a molecular size of 41 kDa was observed in culture media collected from CGTH W-1, SW 579, and RO82 W-1 cells. Conclusions Three thyroid cancer cell lines were established, which may provide material for future thyroid cancer research. © 1996 Wiley-Liss, Inc.

Establishment of xenografts and cell lines from well-differentiated human thyroid carcinoma.

Our aim was to establish Chinese human thyroid cancer cell lines and to provide the material for thyroid cancer research. We collected thyroid cancer tissues from 93 patients with thyroid cancer for developing the primary culture. Thyroid cancer tissues were verified by frozen sections during the operations, then subjected to primary cell culture. During the first several passages, fibroblasts were removed by selective attachment. From the 93 cancer tissues used, two follicular cancer tissues from the metastatic area (CGTH W-1, CGTH W-2) and one papillary thyroid cancer tissue (CGTH W-3) could be passed over 50 times. Cellular transformation with loss of contact inhibition occurred during passages 5-8. Electron microscopic studies of the CGTH W-1 cell line showed the presence of an abundance of mitochondria and Golgi complex. Presence of microvilli with interdigitations between neighboring cells were found in CGTH W-2 and CGTH W-3 cell lines. Severe combined immunodeficient (SCID) mice were used to determine whether these cells were tumorigenic. Two months after transplantation of 1 x 10(7) CGTH W-1, 2, and 3 cells into SCID mice, subcutaneous tumors approximately 2-2.5 cm in size were clearly visible. After affinity cross-linking an IGF-I with insulin-like growth factor binding proteins complex corresponding to a molecular size of 41 kDa was observed in culture media collected from CGTH W-1, SW 579, and RO82 W-1 cells. Three thyroid cancer cell lines were established, which may provide material for future thyroid cancer research.