Oil Of Thymus Vulgaris Research Articles

Exposure to volatiles of essential oils alone or under hypobaric treatment to control postharvest gray mold of table grapes

After harvest, table grapes can easily undergo fungal spoilage, which is mainly caused by Botrytis cinerea, the causal agent of gray mold. To reduce such losses, table grapes are usually treated with conventional fungicides during the season, and cold stored in the presence of sulfur dioxide. However, these applications are not permitted in organic agriculture, and at the same time, there is a growing demand from consumers for fresh fruit free from pesticide residues. The application of essential oils and hypobaric treatments are promising alternatives to sulfur dioxide with minimal environmental impacts and limited concerns about human health risks. The aim of this study was to determine the effectiveness for control of postharvest gray mold of table grapes of 24-h exposure to volatiles of essential oils of Rosmarinus officinalis (rosemary), Mentha piperita (peppermint), and Thymus vulgaris (thyme) individually and in combinations with hypobaric treatment at 50kPa (0.5atm). Exposure to volatiles of rosemary essential oils under atmospheric pressure and hypobaric conditions reduced by around 65% the incidence and McKinney’s Index of gray mold for table grapes that were then stored at room temperature for 9 d and 5 d, respectively, or that were stored at 4°C for 7 d and followed by 3 d shelf life at 20°C. Peppermint essential oils similarly controlled gray mold for grapes stored at room temperature and under hypobaric conditions for 24h. Panel tasting revealed perception of the essential oils soon after the treatments and 24h later for grape berries exposed to vapors of rosemary, peppermint, and Lavandula×ibrida (lavender). Then 48h after treatment, the rosemary and peppermint essential oils were no longer perceived on grapes stored at 4°C and at 20°C. Exposure to volatiles of the rosemary and peppermint essential oils alone or in combination with hypobaric treatment might represent an innovative method to control postharvest gray mold of table grapes, although at least 48h were needed between exposure to volatiles of essential oils and presentation to consumers.

Evaluation and comparison of analgesic activity of essential oil of Rosmarinus officinalis and Thymus vulgaris: an experimental study

Background: Since the time of stone age herbs in its natural forms are used for a variety of medicinal purposes including the treatment of pain. Research in herbal medicine is in a relatively early phase. Herbal approaches usually have been pursued because of the perception that these therapies are gentler and cause fewer side effects than allopathic treatment. The aim was to evaluate and compare the analgesic activity of essential oil of Rosmarinus officinalis and Thymus vulgaris in wistar rats.Methods: Wistar rats and hot plate method was used for this study. Control group (Group A) of 6 animals was given normal saline. Group B of 6 animals was given standard analgesic drug, aspirin (200mg/kg body weight). Group C of 6 rats was given REO at 100, 200, 300 and 400 mg/kg body weight on day 1, 2, 3 and 4 while Group D of 6 animals was given TEO at doses of 100, 200 and 300 mg/kg body wt. The analgesic activity of REO and TEO in terms of Response Time were noted and depicted in tabular form.Results: Results were analyzed by one-way-Anova and Tukey tests. Analgesic effect of Rosemary oil found to be statistically significant at all four doses, 100, 200, 300 and 400mg/kg body wt compared to control group. TEO was found significantly analgesic at 100 and 200mg/kg body wt compared to control group but at 300mg/kg body it was found lethal. REO was found superior.Conclusions: Analgesic effect of REO and TEO was not comparable to aspirin even at higher concentration like 400mg/kg (REO) but it was significantly more compare to control group.

Inhibitory effect of Thymus vulgaris and Origanum vulgare essential oils on virulence factors of phytopathogenic Pseudomonas syringae strains

Pseudomonas syringae is a phytopathogenic bacterium that causes lesions in leaves during the colonisation process. The damage is associated with production of many virulence factors, such as biofilm and phytotoxins. The essential oils of Thymus vulgaris (thyme) and Origanum vulgare (oregano) have been demonstrated to inhibit P.syringae. The aim of this study was to investigate the effects of T.vulgaris and O.vulgare essential oils on production of virulence factors of phytopathogenic P.syringae strains, including anti-biofilm and anti-toxins activities. The broth microdilution method was used for determination of MIC and biofilm inhibition assays. Coronatine, syringomycin and tabtoxin were pheno- and genotypically evaluated. Both oils showed good inhibitory activity against P.syringae, with MIC values from 1.43 to 11.5mg·ml-1 for thyme and 5.8 to 11.6mg·ml-1 for oregano. Biofilm formation, production of coronatine, syringomycin and tabtoxin were inhibited by thyme and oregano essential oil in most strains. The results presented here are promising, demonstrating the bactericidal activity and reduction of virulence factor production after treatment with thyme and oregano oil, providing insight into how they exert their antibacterial activity. These natural products could be considered in the future for the control of diseases caused by P. syringae.

Etude De L’Activité Antioxydante Des Huiles Essentielles De Plantes Aromatiques Et Médicinales Marocaines

Morocco is a traditional supplier of medicinal and aromatic plants to the world. This activity has resulted to the exploitation of wild plants than dried plants for herbal needs and food flavors. Many species are used for the production of plant extracts and other aromatic essences mainly for the pharmaceutical and cosmetics industry. Among these metabolites, there are essential oils that are mixtures of aromatic substances present in the form of tiny droplets in the leaves, fruit peel, resin, branches, and wood. These oils, however, is endowed with antioxidant properties. This study aims to evaluate the antioxidant activity of essential oils of three moroccan aromatic and medicinal plants: Thymus vulgaris, Mentha spicata, and Citrus limonum. The oil extraction was performed by steam distillation in a Clevenger type apparatus. Also, the antioxidant activity of these oils was evaluated by the method of DPPH (2,2-diphenyl-1-picrylhydrazyl), in comparison with the antioxidant synthetic, ascorbic acid. In the present work, the results showed that the antioxidant activity of three essential oils studied by the trapping method of free radical is moderate. Also, the oil of Thymus vulgaris has a strong activity which exceeds that of ascorbic acid. This was followed by the oil of Mentha spicata, while the lowest activity was observed in Citrus limonum.

In Vitro Antimicrobial Activity of Thymus vulgaris Essential Oil Against Major Oral Pathogens.

The objective of present investigation was to determine antimicrobial activity of Thymus vulgaris oil on some oral pathogens. Thymus vulgaris oil was prepared by hydrodistillation and tested against 30 clinical isolates of each of Streptococcus pyogenes, Streptococcus mutans, Candida albicans, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans, prepared from related oral infections using agar disk diffusion and broth microdilution methods. Thymus vulgaris oil at concentrations of 16 to 256 μg/mL exhibited strong inhibitory activity on all clinical isolates producing inhibition zones of 7.5 to 42 mm as measured by agar disk diffusion method. Streptococcus pyogenes and Streptococcus mutans were the most sensitive isolates with minimum inhibitory concentrations of 1.9 and 3.6 μg/mL, respectively. The minimum inhibitory concentration values for C albicans, A actinomycetemcomitans, and P gingivalis were 16.3, 32, and 32 μg/mL, respectively.

Identification of the antibacterial efficacy of ethanolic extracts and oils of some medicinal plants against the growth of Escherichia coli invitro

The present study had thrown the light on the in vitro antimicrobial potential of the ethanolic extract of four local medicinal plants; Terminalia chebula, Lawsonia inermis , Origanum vulgare, Thymus vulgaris, and essential oils of five types of medicinal plants; Elettaria cardamomum, Eugenia caryophyllus, Trigonella foenum-graecum, Linum usitatissimum, Brassica nigra against the growth of pathogenic Escherichia coli (E. coli). The antibacterial activity was carried out by using agar well diffusion technique in Mueller-Hinton agar.
 The results were obtained by measured the zone of inhibition (mm) around the well that could be exhibited by each plant extract and oil following incubation of bacterial plates and expressed as mean±Standard error (SE). Ethanolic extract of Terminalia chebula was possessed the strongest antibacterial effect among the tested plant extracts, followed by Origanum vulgare and Lawsonia inermis extracts. Escherichia coli was not affected by Thymus vulgaris extract. On the other hand, Escherichia coli was variably susceptible to tow of the used essential plant oils; Elettaria cardamomum and Eugenia caryophyllus, whereas the other three plant oils were not active any more.

English

This study aimed to evaluate the antifungal activity of essential oils of Thymus vulgaris L., Cinnamomum zeylanicum B. and Mentha piperita L. on some saprophytic fungi. Essential oils were extracted by hydro-distillation, and chemical composition was analysed by gas chromatography coupled with mass spectrometry (GC-MS). T. vulgaris had as major components, Thymol (35.12%), p-cymene (25.36%) and γ–terpinene (12.48%). E-B-Caryophyllane (21.82%), E-Cinnamaldehyde (13.03%) and eugenol (12.15%) were primary in C. zeylanicum. Menthol (33.59%), menthone (18.47%) and α-pinene (8.21%) were primary in M. piperita. Applying the micro-atmospheric method, essential oils were tested against Rhizopus oryzae Went & Prins, Rhizopus stolonifer Ehrenb, Aspergillus tamarii Taka, Aspergillus parasiticus Speare, Aspergillus flavus Link and Talaromyces purpureogenus purpureogenum. The minimum inhibitory concentrations were 3 to 8, 5 to 16 and 13 to 23 μL/75mL air space for T. vulgaris, C. zeylanicum and M. piperita, respectively. Means of percentage inhibition were compared through one-way ANOVA by the Tukey test. Scanning electron microscopy revealed fungal cell wall deformation after exposure to essential oil vapour. These essential oils can be exploited as alternatives to synthetic food preservatives. Key words: Essential oil, Aspergillus, Rhizopus, Talaromyces, fungal morphology, food preservation.

Insecticidal toxicities of carvacrol and thymol derived from Thymus vulgaris Lin. against Pochazia shantungensis Chou & Lu., newly recorded pest

The insecticidal toxicities of five essential oils against Pochazia shantungensis adults and nymphs, newly recorded pests, were evaluated. The LC50 values of Thymus vulgaris, Ruta graveolens, Citrus aurantium, Leptospermum petersonii and Achillea millefolium oils were recorded as 57.48, 84.44, 92.58, 113.26 and 125.78 mg/L, respectively, against P. shantungensis nymphs using the leaf dipping bioassay, and 75.80, 109.86, 113.26, 145.06 and 153.74 mg/L, respectively, against P. shantungensis adults using the spray bioassay method. Regarding volatile components identified in T. vulgaris oil, the LC50 values of carvacrol and thymol using the leaf dipping bioassay against P. shantungensis nymphs were 56.74 and 28.52 mg/L, respectively. The insecticidal action of T. vulgaris oil against P. shantungensis could be attributed to carvacrol and thymol. Based on the structure-toxicity relationship between thymol analogs and insecticidal toxicities against P. shantungensis nymphs similar to the LC50 values against P. shantungensis adults, the LC50 values of thymol, carvacrol, citral, 2-isopropylphenol, 3-isopropylphenol, and 4-isopropylphenol were 28.52, 56.74 and 89.12, 71.41, 82.49, and 111.28 mg/L, respectively. These results indicate that the insecticidal mode of action of thymol analogs may be largely attributed to the methyl functional group. Thymol analogues have promising potential as first-choice insecticides against P. shantungensis adults and nymphs.

Effect of Vermicompost on Growth, Essential Oil, and Health of Thymus Vulgaris

ABSTRACTIn this study, the effect of various vermicompost substitutions (0, 25, 50, and 75%) was investigated on germination, growth and development, photosynthetic parameters, the essential oil content, and disease resistance of Thymus vulgaris. Results showed that 25% vermicompost substitution promoted the best seedling emergence indices, whereas maximum length, fresh and dry weight of aerial parts and root, chlorophyll and carotenoid contents, photosynthetic efficiency, and the highest essential oil content were observed in 50% vermicompost substitution. Vermicompost was an effective biocontrol agent against Fusarium oxysporum and Phytophthora infestans. Suppression of pathogens by vermicompost was enhanced progressively with increasing rates of substitution of vermicompost, and the highest suppression obtained in 75% vermicompost substitution. The disease protection correlated to development of activities of defense-related enzymes including β-1,3-glucanase, phenylalanine ammonialyase, polyphenol oxidase and peroxidase, and total phenolics content. These results suggest the potential of vermicompost in promoting of plant yield as well as inducing systemic resistance in Thymus vulgaris.

In Vitro Study of the Bacterial Anti-Bioresistance and the Use of Some Medicinal Plants in Avian therapy

This study addresses the problem of pathogenic bacteria resistant to antibiotics isolated from infected chickens and the use of medicinal plants and their bioactive substances in vitro. On 75 strains isolated from a dead chickens belonging to the three families the Enterobactereaceae; the Staphylococeae; the Pseudomonaceae bacteria. The antibiogram tests were used to select ten resistant bacteria strains in this study. Ethonobotanical study was done to select the most medicinal plants used for the therapy of animals in Algeria. The essential oils of six medicinal plants were extracted by Hydro distillation (Clevenger). The plants with their yields in essential oils are: Thymus vulgaris (2.75%), Salvia officinalis (2.50%), Rosmarinus officinalis (2.43%), Thymus capitatus (1.82%), Ruta chalepensis (0.93%), Artemisia herba alba (0.90%). By measuring the activity of the oils on agar medium, this test has provided the following results: The essential oils of different plants gave the diameters of inhibition zones between 0 mm and 53.33 ± 1.53 mm, for the 5 μl discs, and between 0 mm and 52.33 ± 2.52 mm, for the 10 μl discs, while discs of 15 μl, those diameters vary between 0 mm and 56.67 ± 1.15 mm. The results of MICS of oils studied are encouraging, oils of Thymus capitatus, Rosmarinus officinalis and Salvia officinalis share a minimum inhibitory concentration (MIC) between 1.25 and 20 (μL.mL-1) with an effect bactericidal/ bacteriostatic, except the oil Salvia officinalis presents a bactericidal effect. The oils of Thymus vulgaris, Artemisia herba alba and Ruta chalepensis have a MIC respectively of 1.25 to 10 (μL.mL-1), 5 to 40 (μL.mL-1), 1.25 and 40 (μL.mL-1). The effect is bactericidal effect for these oils.

Essential oils of Origanum compactum and Thymus vulgaris exert a protective effect against the phytopathogen Allorhizobium vitis

Essential oils of Origanum compactum and Thymus vulgaris exert a protective effect against the phytopathogen Allorhizobium vitis

Efficiency of various systems of antiparasitic prophylaxis in a flock of sheep

The aim of the study was to evaluate the efficiency of various methods of antiparasitic prophylaxis using a conventional chemical agent and a formulation containing plant essential oils. The animal material consisted of 139 mother ewes of two synthetic prolific meat lines: bcp and scp. During the whole experiment, the animals were kept in indoor management system with uniform environmental conditions. The experiment included ewes lambing within the period of 12 consecutive days. Using the results of coproscopic tests performed after lambing, the animals were randomised into three study groups: Group A - wormed with an albendazole-containing preparation (50 sheep), Group L - receiving a lick with antiparasitic essential oils (48 sheep) and Group C - control group, not wormed (41 sheep). Two antiparasitic agents were used. A chemical agent containing albendazole. The procedure was performed on day 35 after lambing, and the dose administered was 5 mg of the active substance per kg bw, which corresponded to 0.5 ml, orally, per 10 kg bw. A natural worming agent was administered in the form of licks containing essential oils of Thymus vulgaris, Allium sativum, Artemisia absinthium, Dryopteris filixmax, Tanacetum vulgare, Cucurbita pepo, Chenopodium ambrosioide, Inula helenium, Peumus boldo, Corallina rubens. The licks were made available to animals on day 21 after lambing. The experiment involved parasitologic tests aimed at the determination of estimated intensity (based of OPG and EPG data) and prevalence of invasions of gastrointestinal parasites in mother ewes. The study material was faeces collected from the rectum on the following dates: after lambing, and on day 28, 42, 56, 70 and 100 of lamb's life. The observations proved that the formulation based on natural plant ingredients had a beneficial influence on the reduction of prevalence of Eimeria and Capillaria. It was also shown that the albendazole-containing chemical preparation is highly efficient in reducing the prevalence of Trichostrongylidae, Nematodirus, as well as Capillaria.

Evaluation of antifungal activity and cytotoxicity of Thymus vulgaris essential oil

Objective: To evaluate the antimicrobial properties of Thymus vulgaris against seven pathogenic fungi strains and its cytotoxic effect on a human breast cancer cell line (MDA-MB-231) Methods: The susceptibility test was carried out in terms of by determining minimal inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) using micro-dilution methods and additional end point by using MTS chromogenic assay. Cytotoxicity activity was assessed using MTT assay, the viability and the IC50 were used to evaluate this test. Results: All tested fungal strains were sensitive to the (Thymus vulgaris essential oil) TEO and this inhibitory effect is dose-dependent. A remarkable (p<0.001) inhibitory effect at concentrations 2.5-10mg/ml for all tested organisms comparable with control was found. The MIC50 values ranged between 0.5 to 5mg/ml. The MFC values varied from 2.5–10 mg/mL and were equal to MIC values for majority of the tested organisms. As far as the antifungal activity is concern, out of the seven strains tested, Candida kefyr exhibited the highest sensitivity with minimal inhibition growth 2.5mg/ml whereas, Aspergillus niger exhibited the lowest inhibition ≥10mg/ml. Thymus vulgaris oil inhibited proliferation of MDA-MD-231 cancer cells in a time- and dose dependent manner. IC50 value of the complex was found to be time dependent. At 24h treatment, the IC50 values ranging between 108-115μg/ml, while at 48h treatment the values were between 71-78μg/ml against cancer cell. Conclusion: Thymus vulgaris essential oil exhibited significant antifungal and cytotoxic effects on living cells. Therefore, it can be used as alternative antifungal natural substances and also play a significant role in discovery of the new drugs.

Chemical composition of essential oil of Thymus vulgaris collected from Saudi Arabian market

ObjectiveTo focus on the analysis of chemical constituents of the Thymus vulgaris L. (locally known as “Zaitra” or “Za'atar”; Family: Lamiaceae) which is available in the market of Saudi Arabia. MethodsThe Zaitra oil was analyzed by gas chromatography-mass spectrometry. The mass spectra were compared with the standard spectra available in National Institute Standard and Technique library. ResultsThe results indicated that the Thymus oil is composed of many chemical compounds including α pinene, thymol and caryophyllene which are biologically active and also used in various diseases. ConclusionsIt can be concluded that the Thymus vulgaris due to presence of many bioactive compounds can be used as a new potential source of medicine for the treatment of various types of illness.

Seasonal variation affects the composition and antibacterial and antioxidant activities of Thymus vulgaris

The chemical composition and pharmaceutical properties of thyme essential oil have been of great interest to researchers and food industries. The aim of the present study was to evaluate the effects of seasonal variation on the composition and antimicrobial and antioxidant activities of the essential oil of Thymus vulgaris, as well as to determine the activity of the main pure compounds. Composition of the essential oil was determined by GC/MS. Antioxidant activity of the essential oil and the pure compounds was tested by DPPH, ABTS, and FRAP methods. Antimicrobial activity of essential oil and pure compounds was evaluated by determination of minimum inhibitory concentration. The major compounds of all tested samples were thymol (38.99–52.92%), p-cymene (14.38–26.58%), γ-terpinene (10.43–19.09%), linalool (2.39–3.56%), and carvacrol (1.87–3.3%). The essential oil gathered in October/2012 showed the highest antioxidant activity and strongest antimicrobial activity against S. Aureus (MIC=20μg/mL), E. Coli (MIC=50μg/mL) and S. typhimurium (MIC=500μg/mL). Furthermore, the above sample contained the highest amount of thymol. Regarding the analyses of pure compounds, only thymol and carvacrol showed free radical scavenging capacity, and strong antimicrobial activity, while thymol was the most effective against S. Aureus (MIC=250μg/mL), E. Coli (MIC=120μg/mL) and S. typhimurium (MIC=60μg/mL). Data suggested the spring time (around 20°C in Brazil) as the appropriate season to collect the essential oil from thyme.

Toxicities of active constituent isolated from Thymus vulgaris flowers and its structural derivatives against Tribolium castaneum (Herbst)

Insecticidal activity of 2-isopropyl-5-methylphenol isolated from Thymus vulgaris oil and its derivatives against Tribolium castaneum adults and larvae were evaluated using contact and fumigant bioassays. To elucidate the structure–activity relationship, 2-isopropyl-5-methylphenol and its derivatives were determined. Based on the LD50 values, 2-isopropylphenol was most toxic to T. castaneum adults and larvae, followed by 4-isopropylphenol, 2-methylphenol, 3-methylphenol, and phenol in contact and fumigant bioassays. These results indicate that the introduction of a functional group such as methyl and isopropyl group into the phenol skeleton has an important influence on contact and fumigant toxicities. Furthermore, 2-isopropyl-5-methylphenol and its derivatives could be used as an alternative to control stored-grain insects.

Antifungal efficacy of plant essential oils against stored grain fungi of Fusarium spp.

The control potential of seven plant essential oils was evaluated against Fusarium proliferatum (Matsushima) Nirenberg and Fusarium verticillioides Sheldon. The fungicidal activity was assessed through microtiter plate assay to determine the minimum inhibitory and fungicidal concentration of essential oils. The essential oil of Mentha arvensis was adjudged as best for inhibiting the fungal growth, while oil of Thymus vulgaris and Anethum graveolens showed high efficacy in terms of fungicidal activity. The oil of M. arvensis and T. vulgaris also showed good inhibition activity in agar disc diffusion assay. M. arvensis essential oil was analysed for its composition using gas chromatography/mass spectrometry revealing menthol (63.18%), menthone (15.08%), isomenthyl acetate (5.50%) and limonene (4.31%) as major components. Significant activity of M. arvensis essential oil against F. proliferatum and F. verticillioides isolates obtained, pave the way for its use as antifungal control agents.

In vitro and in vivo efficacy of carvacrol against Echinococcus granulosus

Currently, benzimidazoles are used as chemotherapeutic agents and as a complement to surgery and PAIR in the treatment of cystic echinococcosis (CE). They are generally applied at high doses causing side effects and, 50% of cases do not respond favorably to such chemotherapy. The use of essential oils obtained by distillation from aromatic plants would be an effective alternative or complementary to the synthetic compounds, because would not bring the appearance of side effects. Carvacrol and his isomer thymol are the main phenolic components from essential oils of Origanum vulgare (oregano) and Thymus vulgaris (thyme). The aim of the present work was to evaluate the in vitro and in vivo efficacy of carvacrol against Echinococcus granulosus metacestodes. For the in vitro assay, protoscoleces and cysts of E. granulosus were incubated with carvacrol at the following final concentrations: 10, 5 and 1μg/ml of carvacrol. The maximum protoscolicidal effect was found with 10μg/ml of carvacrol. Results of viability tests were consistent with the structural and ultrastructural damage observed in protoscoleces. Ultrastructural studies revealed that the germinal layer of cysts treated with carvacrol lost the multicellular structure feature. In the clinical efficacy study, a reduction in cyst weight was observed after the administration of 40mg/kg of carvacrol during 20days in mice with cysts developed during 4 months, compared to that of those collected from control mice. Given that the in vivo effect of carvacrol was comparable with the treatment of reference with ABZ and the fact that is a safe compound, we postulated that carvacrol may be an alternative option for treatment of human CE.

Chemical Composition antimicrobial and free radical scavenging activity of essential oil from leaves of Renealmia thyrsoidea (Ruiz Pav.) Poepp. Endl.

Renealmia thryrsoidea (Ruiz & Pav.) Poepp. & Endl is a plant used by the Amazonian indigenous people of Ecuador for its various medicinal properties. Its leaves exhibit a remarkable aroma with a hint of spiciness. The essential oil extracted from its leaves was analyzed by GM/MS, using two systems with columns of different polarity, in both was confirmed the presence of terpinolene (26.32%), α-felandrene (17.16%), γ-terpinene (6.55%), β-pinene (5.97%) and p-cymol (4.70%). Free radical scavenging activity was analyzed through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) spectrophotometric methods ; by applying ABTS method , the activity was comparable to that of the essential oil of Thymus vulgaris. The trials of antimicrobial activity show a strong inhibition against Gram negative bacteria as Escherichia coli and Pseudomonas aeruginosa. Key words: Renealmia thryrsoidea, essential oil, DPPH, ABTS, minimum inhibitory concentration (MIC).

Composition of Essential Oil of Thymus vulgaris grown in Oman

Thymus vulgaris L. belongs to the Lamiaceae family and has a pleasant smell. It is native to the Westren Mediterranean region and southern Italy. The fresh plant material was collected in the month of April from Al Jabal Al Abyadh in Sharqiya region of Oman. The aerial parts of T. vulgaris were separated from fresh plant material and essential oil was isolated by steam distillation method using Clevenger's apparatus. The oil was analyzed by GC-MS. A total of eighteen constituents were identified, the major constituent was durenol (56.2 %). Other main constituents were thymol (15.04 %), p-cymene (8.69 %), δ-terpinene (5.24 %), and α-pinene (2.65 %).