Throughfall Research Articles

Drip heterogeneity and the impact of decreased flow rates on the vadose zone fauna in Ciur‐Izbuc Cave, NW Romania

AbstractThe aim of this study was to better understand the unsaturated zone of a karst massif habitat for aquatic fauna by defining patterns of cave drip flow rates. Dripping was logged over a period of 4 years at three points inside Ciur‐Izbuc Cave (north‐western Romania). Drip rates have been analysed at different scales and also compared with rainfall. The results show the individual behaviour of drips on daily, monthly, and yearly timescales and no significant correlations between the three drip points or between them and rainfall. Yearly differences are statistically significant, showing large changes in drip behaviour over the study period. The results point to two different aspects of the water flow through the vadose zone: (a) a space and time heterogeneity raising questions about related stress for the groundwater invertebrates inhabiting this zone and (b) relative uniformity given by signals at certain time intervals that explain species diversity preservation in such a heterogeneous environment. Conservation concerns, and the need for long‐term monitoring of fauna and hydrological processes, are stressed.

Modelling vaporised hydrogen peroxide efficacy against mono-species biofilms

This pilot study investigates a novel approach towards efficacy testing of antimicrobial cleaning agents; focusing primarily on hydrogen peroxide vapour (HPV). Contaminated surfaces are recognised modes of pathogen transmission within healthcare environments and increase the risk of pathogen acquisition in newly admitted patients. Studies have shown these pathogens can survive on surfaces for extended periods of time in spite of cleaning. This resilience is characteristic of biofilm formation and recent publications have identified their presence in hospitals. In this study, biofilm models comprised of multidrug-resistant organisms (MDROs) were generated using a drip flow reactor and exposed to HPV decontamination. The MDROs included Acinetobacter baumannii, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus aureus. Upon exposure, samples were periodically removed and enumerated to generate kill curves for each species. Consequently revealing any inherent resistances; such as catalase-producing organisms which expressed reduced susceptibility. Epifluorescence microscopy revealed an abundance of viable and non-viable microcolonies before and after decontamination, respectively. Greater than 6-Log10 reduction was achieved within a 100 minutes exposure time. This pilot study puts forward a potential methodology for testing antimicrobial agents against biofilms and supports the efficacy of HPV.

Relationships between rainfall and throughfall in a secondary forest in southeastern Brazil: an evaluation of different statistical models

ABSTRACT Throughfall (TF) is influenced by different meteorological conditions, which can result in high spatial and temporal variability, when interacting with vegetation and mutually with each other. This study aimed to evaluate rainfall (RF) influence on TF, as well as to describe the behavior of these variables in an area dominated by Pinus elliottii in southeastern Brazil, by exploring different statistical models proposed in the literature. For this, RF and TF data were recorded in 24 rainfall events by 180 gauges distributed in six 10 x 10 m plots. The results indicate a significant influence of RF volume on response variables [TF volume (TFmm), TF fraction (TF%) and coefficient of variation of TF (CVTF)]. While the linear model presented the best fit for TFmm , the non-linear models had better results for TF% and CVTF as a function of RF, allowing the identification of distinct behaviors for different RF volumes. In general, it was verified that RF is the main source of variability in TF estimates in the study area. However, it should be noted that other variables may be acting simultaneously on TF% and CVTF, in which 45.4 and 38.1% of the variation, respectively, remain unexplained, requiring complementary studies to identify and quantify the influence of other factors.

A Versatile Strategy for Characterization and Imaging of Drip Flow Microbial Biofilms.

The inherent architectural and chemical complexities of microbial biofilms mask our understanding of how these communities form, survive, propagate, and influence their surrounding environment. Here we describe a simple and versatile workflow for the cultivation and characterization of model flow-cell-based microbial ecosystems. A customized low-shear drip flow reactor was designed and employed to cultivate single and coculture flow-cell biofilms at the air-liquid interface of several metal surfaces. Pseudomonas putida F1 and Shewanella oneidensis MR-1 were selected as model organisms for this study. The utility and versatility of this platform was demonstrated via the application of several chemical and morphological imaging techniques-including matrix-assisted laser desorption/ionization mass spectrometry imaging, secondary ion mass spectrometry imaging, and scanning electron microscopy-and through the examination of model systems grown on iron substrates of varying compositions. Implementation of these techniques in combination with tandem mass spectrometry and a two-step imaging principal component analysis strategy resulted in the identification and characterization of 23 lipids and 3 oligosaccharides in P. putida F1 biofilms, the discovery of interaction-specific analytes, and the observation of several variations in cell and substrate morphology present during microbially influenced corrosion. The presented workflow is well-suited for examination of both single and multispecies drip flow biofilms and offers a platform for fundamental inquiries into biofilm formation, microbe-microbe interactions, and microbially influenced corrosion.

Selenate removal in biofilm systems: effect of nitrate and sulfate on selenium removal efficiency, biofilm structure and microbial community

AbstractBACKGROUNDSelenium (Se) discharged into natural waterbodies can accumulate over time and have negative impacts on the environment. Se‐laden wastewater streams can be treated using biological processes. However, the presence of other electron acceptors in wastewater, such as nitrate (NO3‐) and sulfate (SO42‐), can influence selenate (SeO42‐) reduction and impact the efficiency of biological treatment systems.RESULTSSeO42‐ removal by biofilms formed from an anaerobic sludge inoculum was investigated in the presence of NO3‐ and SO42‐ using drip flow reactors operated continuously for 10 days at pH 7.0 and 30 °C. The highest total Se (∼60%) and SeO42‐ (∼80%) removal efficiencies were observed when the artificial wastewater contained SO42‐. A maximum amount of 68 μmol Se cm‐2 was recovered from the biofilm matrix in SO42‐ + SeO42‐ exposed biofilms and biofilm mass was 2.7‐fold increased for biofilms grown in the presence of SO42‐. When SeO42‐ was the only electron acceptor, biofilms were thin and compact. In the simultaneous presence of NO3‐ or SO42‐, biofilms were thicker (> 0.6 mm), less compact and exhibited gas pockets.CONCLUSIONThe presence of SO42‐ had a beneficial effect on biofilm growth and the SeO42‐ removal efficiency, while the presence of NO3‐ did not have a significant effect on SeO42‐ removal by the biofilms. © 2018 Society of Chemical Industry

ИССЛЕДОВАНИЕ ВЫСОКОТЕМПЕРАТУРНЫХ СВОЙСТВ ЖЕЛЕЗОРУДНЫХ МАТЕРИАЛОВ В ЛАБОРАТОРНЫХ УСЛОВИЯХ

The aim of the study is to simulate in laboratory conditions the behavior of iron ore materials in the zone of softening-melting DP and drip flow using an integrated method developed at the Institute of Ferrous Metallurgy. It is shown that the widespread idea of the formation in the blast furnace of primary slag melt based on FeO takes place mainly in the initial period of slag formation and is true for low basicity iron ore materials. For pellets, the nature of the formation of liquid phases differs significantly from the agglomerate. Experimentally established changes in the composition of the slag melt as heating. It is shown that under the conditions of temperature and heat treatment of raw materials, each temperature has its own composition of the liquid phase. When the pellets are melted, the primary melt is formed in the temperature range of 1330–13600C, in which the proportion of primary slag is 16–25%. Melt from high-silicon lumpy ore is formed at high temperatures of 1490-15200С. The mass of the primary slag with 42-48% FeO is 4-8% by weight of iron ore materials. The main part of the melt hangs on the coke nozzle on the coke layer at temperatures above 16000C. Melts formed from mixtures of iron-containing materials, as a rule, have averaged characteristics. The observed patterns make it possible to predict the behavior and properties of multicomponent charge mixtures in a blast furnace.

Effect of Vertical Distribution of Soil Water on N<sub>2</sub>O Emission under Drip Irrigation

N2O emission has obvious water effect, but the current research is not deep enough. The soil wetting mode of drip irrigation technology is obviously different from that of conventional irrigation. Using the method of soil box indoor simulation, the N2O emission under different soil vertical water content was analyzed. Hydrus Software was used to simulate the soil wetting body under different drip irrigation technical parameters, the relationship between the combination of drip irrigation technical parameters and soil vertical water content was studied, and then the relationship between the N2O emission and the combination of drip irrigation technical parameters was proposed. The results showed that soil N2O emission flux increased with the increase of soil moisture, and the maximum emission flux was three times as much as the minimum emission flux. Under the condition of uniform distribution of soil moisture, soil N2O emission flux was smaller than that under non-uniform distribution of soil moisture. Hydrus software simulation results show that drip flow rate is 2.0 L/h, the irrigation period is 5 days, the irrigation quota is 12 mm, and the soil N2O emission flux is the largest. Adjusting the combination of technical parameters of drip irrigation can reduce soil N2O emission flux.

Oak trees decline; a sign of climate variability impacts in the west of Iran

The Persian oak, Quercus brantii, trees in the Zagros region of Western Iran have been in decline since 2000. The decline is assumed to be highly connected with changes in meteorological parameters. Our objectives were to quantify the long-term trends in meteorological parameters and reference evapotranspiration (ET0) in the Zagros region and estimate ecohydrological parameters highly affected by climate variability and related to rainfall interception (I) process (i.e., canopy storage capacity (S), the ratio of mean evaporation rate from the wet canopy to the mean rainfall intensity (E/R), and the free throughfall coefficient (p)). Long-term (1961-2010) changes in air temperature (T), precipitation (P), and wind speed (WS) were obtained from six synoptic meteorological stations located in the region. Throughfall (TF) was measured using the sixteen rain gauges randomly located underneath the crown of the five individual trees. P was measured using rain gauges fixed in an open space nearby to the oak trees .I was computed as the difference between P and TF. From 2000 to 2010, meteorological parameters and ET0 changed slightly; T, WS, and ET0 increased (+0.6 ˚C, +0.4 m.s-1, +0.25 mm.day-1, respectively), while P decreased (-60 mm).When climate patterns between 1961-2010 were analyzed for 6 synoptic weather stations, P significantly decreased significantly at one station, whereas T significantly increased at two stations and significantly decreased at another. I was estimated to be 40% and 25% within in-leaf and leaf-less periods, respectively. During the in-leaf period, the mean values of S, E/R, and p were roughly estimated to be 1 mm, 0.22, and 0.23, respectively. Our results indicate that the Zagros region is getting warmer and oak trees will indisputably experience reduction in the available water because of increased evaporative loss.

Spatio-temporal variation of throughfall in a hyrcanian plain forest stand in Northern Iran

Abstract Elucidating segregation of precipitation in different components in forest stands is important for proper forest ecosystems management. However, there is a lack of information on important rainfall components viz. throughfall, interception and stemflow in forest watersheds particularly in developing countries. We therefore investigated the spatiotemporal variation of important component of throughfall for a forest stand in a Hyrcanian plain forest in Noor City, northern Iran. The study area contained five species of Quercus castaneifolia, Carpinus betulus, Populus caspica and Parrotia persica. The research was conducted from July 2013 to July 2014 using a systematic sampling method. Ninetysix throughfall collectors were installed in a 3.5 m × 3.5 m grid cells. The canopy covers during the growing/leaf-on (i.e., from May to November) and non-growing/leaf-off (i.e., from December to March) seasons were approximately 41% and 81%, respectively. The mean cumulative throughfall during the study period was 623±31 mm. The average throughfall (TF) as % of rainfall (TFPR) during leaf-on and leaf-off periods were calculated 56±14% and 77±10%, respectively. TF was significantly (R2 = 0.97, p = 0.00006) correlated with gross precipitation. Percent of canopy cover was not correlated with TF except when gross precipitation was <30 mm. A comparison between leaf-off and leaf-on conditions indicated a significantly higher TFPR and corresponding hotspots during leaf-on period. TFPR also differed between seasons with a maximum amount in winter (82%). The results of the study can be effectively used by forest watershed managers for better perception of hydrological behavior of the Hyrcanian forest in the north of Iran under different silvicultural circumstances leading to getting better ecosystem services.

Influence of forest and shrub canopies on precipitation partitioning and isotopic signatures

AbstractOver a 4‐month summer period, we monitored how forest (Pinus sylvestris) and heather moorland (Calluna spp. and Erica spp.) vegetation canopies altered the volume and isotopic composition of net precipitation (NP) in a southern boreal landscape in northern Scotland. During that summer period, interception losses were relatively high and higher under forests compared to moorland (46% of gross rainfall [GR] compared with 35%, respectively). Throughfall (TF) volumes exhibited marked spatial variability in forests, depending upon local canopy density, but were more evenly distributed under heather moorland. In the forest stands, stemflow was a relatively small canopy flow path accounting for only 0.9–1.6% of NP and only substantial in larger events. Overall, the isotopic composition of NP was not markedly affected by canopy interactions; temporal variation of stable water isotopes in TF closely corresponded to that of GR with differences of TF‐GR being −0.52‰ for δ2H and −0.14‰ for δ18O for forests and 0.29‰ for δ2H and −0.04‰ for δ18O for heather moorland. These differences were close to, or within, analytical precision of isotope determination, though the greater differences under forest were statistically significant. Evidence for evaporative fractionation was generally restricted to low rainfall volumes in low intensity events, though at times, subtle effects of liquid–vapour moisture exchange and/or selective transmission though canopies were evident. Fractionation and other effects were more evident in stemflow but only marked in smaller events. The study confirmed earlier work that increased forest cover in the Scottish Highlands will likely cause an increase in interception and green water fluxes at the expenses of blue water fluxes to streams. However, the low‐energy, humid environment means that isotopic changes during such interactions will only have a minor overall effect on the isotopic composition of NP.

Small changes in Cu redox state and speciation generate large isotope fractionation during adsorption and incorporation of Cu by a phototrophic biofilm

Despite the importance of phototrophic biofilms in metal cycling in freshwater systems, metal isotope fractionation linked to metal adsorption and uptake by biofilm remains very poorly constrained. Here, copper isotope fractionation by a mature phototrophic biofilm during Cu surface adsorption and incorporation was studied in batch reactor (BR) and open drip flow reactor (DFR) systems at ambient conditions. X-ray Absorption Spectroscopy (both Near Edge Structure, XANES, and Extended Fine Structure, EXAFS) at Cu K-edge of the biofilm after its interaction with Cu in BR experiments allowed characterizing the molecular structure of assimilated Cu and quantifying the degree of CuII to CuI reduction linked to Cu assimilation. For both BR and DFR experiments, Cu adsorption caused enrichment in heavy isotope at the surface of the biofilm relative to the aqueous solution, with an apparent enrichment factor for the adsorption process, ε65Cuads, of +1.1±0.3‰. In contrast, the isotope enrichment factor during copper incorporation into the biofilm (ε65Cuinc) was highly variable, ranging from −0.6 to +0.8‰. This variability of the ε65Cuinc value was likely controlled by Cu cellular uptake via different transport pathways resulting in contrasting fractionation. Specifically, the CuII storage induced enrichment in heavy isotope, whereas the toxicity response of the biofilm to Cu exposure resulted in reduction of CuII to CuI, thus yielding the biofilm enrichment in light isotope. EXAFS analyses suggested that a major part of the Cu assimilated by the biofilm is bound to 5.1±0.3 oxygen or nitrogen atoms, with a small proportion of Cu linked to sulfur atoms (NS<0.6) of sulfhydryl groups. XANES analyses showed that the proportion of CuIIvs CuI, compared to the initial CuII/CuI ratio, decreased by 14% after the first hour of reaction and by 6% after 96h of reaction. The value of ε65Cuinc of the biofilm exhibited a similar trend over time of exposure. Our study demonstrates the complexity of biological processes associated with live phototrophic biofilms, which produce large and contrasting isotope fractionations following rather small Cu redox and speciation changes during uptake, storage or release of the metal, i.e., favoring heavy isotopes during complexation with carboxylate ligands and light isotopes during reduction of CuII-O/N to CuI-sulfhydryl moieties.

Adhesion forces of biofilms developed in vitro from clinical strains of skin wounds

A biofilm is a very complex consortium formed by a mix of different microorganisms, which have become an important health problem, because its formation is a resistance mechanism used by bacteria against antibiotics or the immune system. In this work, we show differences between some physicochemical properties of biofilms in mono- and multi-species, formed by bacteria from clinical samples of infected chronic wounds. Of the most prevalent bacteria in wounds, two mono- and one multi-species biofilms were developed in vitro by Drip Flow Reactor: one biofilm was developed by S. aureus, other by P. aeruginosa, and a third one by the mix of both strains. With these biofilms, we determined microbial growth by plate counting, and their physicochemical characterization by Atomic Force Microscopy, Raman Micro-Spectroscopy and Scanning Electron Microscopy. We found that the viability of S. aureus was less than P. aeruginosa in multi-species biofilm. However, the adhesion force of S. aureus is much higher than that of P. aeruginosa, but it decreased while that of P. aeruginosa increased in the multi-species biofilm. In addition, we found free pyrimidines functional groups in the P. aeruginosa biofilm and its mix with S. aureus. Surprisingly, each bacterium alone formed single layer biofilms, while the mix bacteria formed a multilayer biofilm at the same observation time. Our results show the necessity to evaluate biofilms from clinically isolated strains and have a better understanding of the adhesion forces of bacteria in biofilm multispecies, which could be of prime importance in developing more effective treatments against biofilm formation.

Estimation of throughfall with changing stand structures for Japanese cypress and cedar plantations

Throughfall (TF) is a critical component of the hydrological and biogeochemical cycles and is greatly influenced by stand structures in forested watersheds. Previous studies have examined the relationships between TF and stand structures for different species and regions. However, there remains acknowledged difficulty in estimation of TF with changing stand structures due to forest management (e.g., thinning) for specific species and regions. This study conducted intensive thinning with 43–50% stem removal at seven experimental plots with various structures (e.g., stand density, canopy cover and basal area) across Japan that were covered by Japanese cypress (Chamaecyparis obtusa Endl.) and cedar (Cryptomeria japonica D. Don) plantations. TF was measured by a set of 20 tipping-bucket rain gauges placed in a lattice-like pattern within the plots before and after thinning. Results showed that during the study periods, thinning caused an increase in the mean TF rate from 63.2±7.0% to 75.4±6.0%. The TF rate was significantly negatively correlated with stand density, canopy cover, and basal area on a stand scale. Additionally, the slope (aTF) of event-based linear TF-Pg (gross rainfall) equation was significantly higher in post-thinning (0.782±0.077) than that in pre-thinning (0.663±0.078), whereas it’s intercept (bTF) showed no significant difference (−0.707±0.810mm in pre-thinning and −0.441±0.607mm in post-thinning). The aTF significantly decreased with increasing stand density and canopy cover, whereas the bTF was not significantly correlated with any forest-structure variables. Further, the TF rate and aTF were estimated, respectively, by their related stand-structural variables in multiple regression models with high determination coefficients and moderate relative errors. The bTF was assumed to be a constant and its mean value obtained from all the experimental plots in each (pre- and post-thinning) period. Thus, the models of stand-scale TF rate and event-based TF amount were developed with input of commonly forest inventories. These models were validated by using the dataset of this study and earlier publications for Japanese cedar and cypress plantations, and showed good fit between the estimated and measured values. These models are practical tools that can be readily used for assessing the changes in TF with changing stand structures at both stand and event-based scales, and have also potential implications in evaluating the spatial TF patterns at catchment scale and exploiting similar models in other species and regions.

Spatio-temporal dynamics of gross rainfall partitioning and nutrient fluxes in shaded-cocoa (Theobroma cocoa) systems in a tropical semi-deciduous forest

Land-use change from forest to cocoa agroforestry and other tree-based farming systems alters the structure of forest stands and influences the magnitude of canopy water fluxes and subsequent bio-element inputs to the forest floor. The partitioning of incident rainfall (IR) into throughfall (TF), stemflow (SF) and canopy interception loss (ILC) and their associated nutrient element concentrations and fluxes was examined along a replicated chrono-sequence: forest, 3, 15 and 30-year-old smallholder shaded-cocoa systems in Ashanti Region, Ghana. Mean annual precipitation during the 2-year observational period (2007 and 2008) was 1376.2 ± 93.8 mm. TF contributed between 76.5–90.4%, and SF between 1.4–1.7% of the annual IR to the forest floor. There were significant differences in IR, TF and SF chemistry. While TF and SF were enriched in phosphorus (1.33–5.67-fold), potassium (1.1–5.69 fold), calcium (1.35–2.65 fold) and magnesium (1.4–2.68 fold) relative to IR, total N (NH4 ++NO3 −) declined (0.5–0.91) of IR values in TF and SF in forest and shaded cocoa systems. Incident rainfall was significantly more acidic than TF and SF in both forest and shaded-cocoa systems. Mean annual total N, P, K, Ca and Mg inputs to the forest floor through IR were 5.7, 0.14, 13.6, 9.43 and 5.6 kg ha−1year−1 respectively. Though an important source of available nutrients for plant growth, incident rainfall provides only a small percentage of the annual nutrient requirements. With declining soil fertility and pervasive low cocoa yields, possible effects of the reported nutrient fluxes on nutrient budgets in cocoa systems merit further investigation. Against the background of increased TF and decreased ILC following forest conversion to shaded-cocoa, it is also recommended that more studies be carried out on rainfall partitioning and its impact on ground water recharge as a way of establishing its influence on the availability of moisture for agriculture in these systems.

Flow regime mapping of aqueous two-phase system droplets in flow-focusing geometries

The recent advent of aqueous two-phase system (ATPS) has shown great potential to rapidly generate microscale aqueous droplets without tedious post-processing. ATPS provides a more biologically friendly and straightforward method to manufacture aqueous droplets compared with conventional oil-water systems and as such, it has been employed in many biomedical applications. Although the cost-effective manufacturing of aqueous droplets has been feasible by direct generation in ATPS, an understanding of the underlying physics of droplet formation in ATPS is still in its infancy. In this paper, we investigated the hydrodynamic behavior and mechanisms of all-aqueous droplet formation in two flow-focusing droplet generators. This study specifically tests whether ATPS in different geometries generates aqueous droplets with different sizes and properties. To achieve these goals, two incompatible polymers, namely polyethylene glycol (PEG) and dextran (DEX), were mixed in water to make ATPS. The influences of inlet pressures and flow-focusing configurations on droplet size, droplet generation frequency, and thread breakup length were quantified. In addition, flow regime mapping for the two different droplet generators at 30° and 90° junction angles was obtained. The results show that droplet size is very susceptible to the junction angle. On the other hand, inlet pressures of the PEG and DEX flows readily control five main flow regimes including PEG Back Flow, DEX Back Flow, Dripping Flow, Jetting Flow and Stratified Flow. It is observed that generated droplets in the Jetting Flow regime are larger in the case of 30°, whereas larger droplets are obtained in the Dripping Flow regime at 90° configuration. The frequency of droplet generation increases and decreases by increasing PPEG and PDEX, respectively. Finally, we characterized flow regimes by the Capillary number (Ca) of the PEG flow.

Solid-state 13C NMR characterization of surface fire effects on the composition of organic matter in both soil and soil solution from a coniferous forest

Wildfires change the chemical composition of soil organic matter (SOM). Since the effects of fires on organic matter (OM) in soil solution are largely unknown, we sought to compare the quality of dissolved organic matter (DOM) and total organic matter (TOM=DOM+particulate OM) between burned and control sites. The sites were subjected to a low-intensity surface fire in a coniferous forest in Germany dominated by spodic Cambisols derived from Triassic sandstone. Soil solutions from three different soil horizons (O, Ah, Bw), and throughfall (TF) were analyzed using solid-state 13C NMR spectroscopy, allowing us to track the initial fire impact on OM vertically through the soil profile and 70days after the fire. In addition, organic layer samples were analyzed by 13C NMR spectroscopy to compare the OM quality. Under control conditions, properties of SOM influence the chemical composition of DOM and TOM in soil solutions. However, with fire, there is an initial increase in aromatic C in SOM, but not in DOM and TOM. Seventy days after the fire treatment, the aromatic C fraction in soil solutions of O and Ah layers increased, possibly due to accelerated oxidation processes, which would make the aromatic C more water-soluble. Our findings highlight the importance of short-term low-intensity fire-induced changes on forest soils that are useful to those seeking to better understand and model the temporal variability in the response of soil chemistry to fire to improve our knowledge of TOM and DOM dynamics in soils.

Factors Influencing Corn Canopy Throughfall at the Row Scale in Northeast China

Core Ideas Throughfall was statistically different among five measurement locations. Cumulative throughfall of 24 to 36 cm from row exceeded that of 0 to 12 cm and 48 to 60 cm by 46 to 88%. Coefficient of variation of throughfall decreased with rainfall amount and increased with leaf area index and plant height. Understanding throughfall (TF) and its influencing factors at crop row scale is a key step to reveal the mechanisms of soil water recharging, soil erosion, and evaporation of intercepted rainfall. The objective of this study was to determine the amount and the variation of TF in relation to plant canopy and rainfall. In this study, TF was measured by a rectangular plexiglass collector at five measurement locations (MLs) with the distances of the (A) 0 to 12 cm, (B) 12 to 24 cm, (C) 24 to 36 cm, (D) 36 to 48 cm, and (E) 48 to 60 cm from corn (Zea mays L.) row during the period of corn plant height growth (HT) approximately from 50 to 250 cm when the leaf area index (LAI) increases from 0.4 to 4.0 in 2013 and 2014. Throughfall increased with total rainfall amount (RA) but decreased with LAI and HT values. The lowest TF values were recorded at the MLs of 0 to 12 cm and 48 to 60 cm near the corn row, and the highest TF values occurred at the ML of 24 to 36 cm at the center of between row areas. The cumulative TF at the ML of 24 to 36 cm exceeded that at the MLs of 0 to 12 cm and 48 to 60 cm by approximately 88 and 46% in 2013 and 2014, respectively. These results have potential values in irrigation scheduling and agrochemical application in the Corn Belt of Northeast China (CBNC).

Effect of silver-loaded PMMA on Streptococcus mutans in a drip flow reactor.

Orthodontic retention has been proposed as a life-long commitment for patients who desire to maintain straight teeth. However, the presence of foreign material increases risk of bacterial colonization and caries formation, of which Streptococcus mutans is a key contributor. Multiple studies have assessed the ability of silver to be added to base plate material and resist attachment of S. mutans. However, it does not appear that long-term washout in connection with biofilm growth under physiologically relevant conditions has been taken into consideration. In this study, silver was added to base plate material and exposed to short- or long-term washout periods. Materials were then assessed for their ability to resist biofilm formation of S. mutans using a drip flow reactor that modeled the human oral environment. Data indicated that silver was able to resist biofilm formation following short-term washout, but long-term washout periods resulted in a lack of ability to resist biofilm formation. These data will be important for future development of base plate materials to achieve long-term antimicrobial efficacy to reduce risk of caries formation and benefit patients in the long term. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2632-2639, 2017.

The influence of sugarcane crop development on rainfall interception losses

The expansion of sugarcane plantations in Brazil has raised concerns regarding its hydrological impacts. One of these impacts is related to rainfall interception, which can be expected to vary in response to substantial changes in canopy structure throughout the cropping cycle. We collected field measurements to determine interception losses and interpreted the observations using an adapted Gash model during different stages of a sugarcane ratoon cropping cycle. Cumulative gross rainfall (PG), throughfall (TF) and stemflow (SF) were measured biweekly, along with vegetation structure measurements of leaf area index (LAI) and plant height. For the first 300days after the first harvest, the cumulative PG of 1095mm was partitioned into 635mm TF (58%) and 263mm SF (24%). The inferred interception loss (IL) was 263mm (24%). There was a gradual and clear increase in IL from 3% to 46% while partitioning between TF and SF also changed during ratoon regrowth. After model parameter optimisation, observed IL was simulated satisfactorily. Model estimates suggested that evaporation from the saturated canopy is the main IL pathway, followed by evaporation after storms. Plant architecture, LAI and meteorological conditions during the cropping cycle appeared the main factors determining IL.

Prevention of Staphylococcus aureus biofilm formation by antibiotics in 96-Microtiter Well Plates and Drip Flow Reactors: critical factors influencing outcomes

Biofilm formation leads to the failure of antimicrobial therapy. Thus, biofilm prevention is a desirable goal of antimicrobial research. In this study, the efficacy of antibiotics (doxycycline, oxacillin and rifampicin) in preventing Staphylococcus aureus biofilms was investigated using Microtiter Well Plates (MWP) and Drip Flow Reactors (DFR), two models characterized by the absence and the presence of a continuous flow of nutrients, respectively. Planktonic culture of S. aureus was exposed to antibiotics for one hour followed by 24 hours incubation with fresh nutrients in MWP or continuous flow of nutrients in DFR. The DFR grown biofilms were significantly more tolerant to the antibiotics than those grown in MWP without the continuous flow. The differences in log reductions (LR) between the two models could not be attributed to differences in the cell density, the planktonic inoculum concentration or the surface-area-to-volume ratios. However, eliminating the flow in the DFR significantly restored the antibiotic susceptibility. These findings demonstrate the importance of considering differences between experimental conditions in different model systems, particularly the flow of nutrients, when performing anti-biofilm efficacy evaluations. Biofilm antibiotic efficacy studies should be assessed using various models and more importantly, in a model mimicking conditions of its clinical application.