Thrombin Inhibitory Capacity Research Articles

Studies on human plasma antithrombin isolated on heparin gel

A purified antithrombin III, prepared from human plasma by affinity chromatography on heparin gels, recently became available. The preparation showed the typical kinetic patterns of antithrombin III and reacted against antithrombin III antiserum. In the present paper, its thrombin inhibitory capacity in plasma was studied after infusion in the dog.

Plasmin, Plasmin Inhibitors and Degradation Products of Fibrinogen in Human Serum during and after Intravenous Infusion of Streptokinase

he variation of plasminogen, plasmin, plasmin inhibitors, fibrinogen and its split products were estimated in sera obtained from three patients before, during and after intravenous infusion (24 hours) of streptokinase.Practically all plasminogen was activated to plasmin within the first four hours of the infusion. The plasmin was recovered mainly as a complex with the ‘immediate inhibitor’ i.e. α2-macroglobulin. This complex was almost completely eliminated within 24 hours, during which time the α2-macroglobulin concentration decreased by about 50 per cent. The α2-macroglobulin did not quite recover its original concentration during the observation period (up to 16 days). The plasminogen recovered its initial concentration within 48 hours after the end of the infusion. No complex formation could be demonstrated between plasmin and the ‘slow inhibitor’ i.e. α1-antitrypsin. The concentration of α1-antitrypsin increased markedly after the infusion of streptokinase.The ‘thrombin inhibiting capacity’ of the macroglobulin fractions after gel filtration varied mainly with the α2-macroglobulin concentration, while the inhibiting capacity of the fractions containing albumin was almost unchanged during the infusion of streptokinase.In spite an excess of α2-macroglobulin in plasma, fibrinogen was degraded to products of varying molecular size. The concentration of the degradation products was highest 4 hours after the beginning of the infusion. Only trace amounts were demonstrable 24 hours after the end of the infusion.