Nerve excitability methods use threshold tracking and conditioning currents to provide information on in situ resting membrane potential and ion channel activity. In this study we propose two techniques for measuring the nerve excitability properties of the Facial Nerve. Two distinct Facial Nerve excitability techniques were developed using consistent surface anatomical landmarks for the Middle Division and Marginal Mandibular branch of the Facial Nerve. The Middle Division was stimulated anterior to the tragus at Zuker’s point (5 cm anterior to the tragus along the line between tragus and the corner of the mouth) with the EMG recording from the Nasalis muscle. The Marginal Mandibular branch was stimulated 1 cm below and 1–2 cm anterior to the angle of the mandible whilst recording from the Depressor Angularis Oris (DAO) muscle. These techniques were performed on ten healthy volunteers to establish reproducibility and normative data. Threshold tracking was performed using QTRAC software and the TROND protocol. Results were compared to Median Nerve-Abductor Pollicis Brevis (APB) motor recordings from age matched healthy controls. Satisfactory recordings were achieved in all participants for both branches. The techniques were both well tolerated. There were no statistically significant differences between excitability parameters recorded from Nasalis and DAO muscles. Compared to the Median-APB data, the Nasalis and DAO both demonstrate a significant decrease in superexcitability ( p < 0.05), decreased S2 accommodation ( p < 0.05), decreased subexcitability ( p < 0.05) and prolonged Relative Refractory Period ( p < 0.05). This study demonstrates the ability to record Facial Nerve axonal excitability from two distinct Facial Nerve branches using techniques which are well tolerated and reproducible. Two recording sites allows for branch comparison and will aid the excitability assessment of incomplete facial palsies in future studies. The excitability properties of the Facial Nerve are profoundly different from those of the Median-APB recordings from healthy controls.