Three-Yellow Chicken Research Articles

A novel highly virulent nephropathogenic QX-like infectious bronchitis virus originating from recombination of GI-13 and GI-19 genotype strains in China

Infectious bronchitis virus (IBV) is one of the most widely spread RNA viruses, causing respiratory, renal, and intestinal damage, as well as decreased reproductive performance in hens, leading to significant economic losses in the poultry industry. In this study, a new IBV strain designated as CK/CH/GX/LA/071423 was successfully isolated from the 60-day-old Three-Yellow chicken vaccinated with H120 and QXL87 vaccines. The complete genome sequence analysis revealed that the CK/CH/GX/LA/071423 strain shared a high similarity of 96.7% with the YX10 strain belonging to the GI-19 genotype. Genetic evolution analysis based on the IBV S1 gene showed that the CK/CH/GX/LA/071423 isolate belonged to the GI-19 genotype. Recombination analysis of the virus genome using RDP and Simplot software indicated that CK/CH/GX/LA/071423 was derived from recombination events between the YX10 and 4/91 vaccine strains, which was supported by phylogenetic analysis using gene sequences from the 3 regions. Furthermore, the S1 protein tertiary structure differences were observed between the CK/CH/GX/LA/071423 and the QXL87 and H120 vaccine strains. Pathogenicity studies revealed that the CK/CH/GX/LA/071423 caused death and led to pale and enlarged kidneys with abundant urate deposits, indicative of a nephropathogenic IBV strain. High virus titers were detected in the trachea, kidneys, and cecal tonsils, demonstrating broad tissue tropism. Throughout the experimental period, the virus positive rate in throat swabs of the infected group reached to 100%. These findings highlight the continued predominance of the QX genotype IBV in Guangxi of China and the ongoing evolution of different genotypes through genetic recombination, raising concerns about the efficacy of current IBV vaccines in providing effective protection to poultry.

Effects of addition of Clitocybe squamulose on nutrients and formation of micro-nanoparticles in Chinese three-yellow chicken soup

Clitocybe squamulose, as a nutritious edible fungus, is widely distributed in Wutai Mountain (Shanxi, China), and is rarely used for stewing chicken soup. Here, the effects of Clitocybe squamulose addition on Chinese three-yellow chicken soup quality were analyzed. The results showed that Clitocybe squamulose addition significantly increased the contents of soluble solid matter, water-soluble protein, total sugar, 5′-GMP (guanosine-5′-monophosphate disodium salt), umami and sweet free amino acids (P < 0.05), increased the absolute value of Zeta potential, and reduced particle size of fat and protein micro-nano particles (MNPs) in chicken soup. Due to Clitocybe squamulose addition improving emulsification performance of chicken soup, MNPs became more stable and evenly dispersed. In summary, Clitocybe squamulose endowed chicken soup with higher nutritional value and improved MNPs distribution in chicken soup. Therefore, Clitocybe squamulose is an excellent natural material that can effectively improve the quality of chicken soup, exhibiting a promising application prospect.

Analysis of Conservation Priorities and Runs of Homozygosity Patterns for Chinese Indigenous Chicken Breeds.

To achieve sustainable development of the poultry industry, the effective conservation of genetic resources has become increasingly important. In the present study, we systematically elucidated the population structure, conservation priority, and runs of homozygosity (ROH) patterns of Chinese native chicken breeds. We used a high-density genotyping dataset of 157 native chickens from eight breeds. The population structure showed different degrees of population stratification among the breeds. Chahua chicken was the most differentiated breed from the other breeds (Nei = 0.0813), and the Wannan three-yellow chicken (WanTy) showed the lowest degree of differentiation (Nei = 0.0438). On the basis of contribution priority, Xiaoshan chicken had the highest contribution to the total gene diversity (1.41%) and the maximum gene diversity of the synthetic population (31.1%). WanTy chicken showed the highest contribution to the total allelic diversity (1.31%) and the maximum allelic diversity of the syntenic population (17.0%). A total of 5242 ROH fragments and 5 ROH island regions were detected. The longest ROH fragment was 41.51 Mb. A comparison of the overlapping genomic regions between the ROH islands and QTLs in the quantitative trait loci (QTL) database showed that the annotated candidate genes were involved in crucial economic traits such as immunity, carcass weight, drumstick and leg muscle development, egg quality and egg production, abdominal fat precipitation, body weight, and feed intake. In conclusion, our findings revealed that Chahua, Xiaoshan, and WanTy should be the priority conservation breeds, which will help optimize the conservation and breeding programs for Chinese indigenous chicken breeds.

Correlation of lipoprotein lipase gene polymorphism and mRNA expression with intramuscular fat content in Baicheng-Oil chicken.

Lipoprotein lipase (LPL) was often taken as a candidate gene for investigating fat metabolism. However, there are few studies on the effect of LPL on intramuscular fat (IMF) deposition in Baicheng oil chicken (BOC) and Three-yellow Chicken (TYC). In this study, we studied the relationship between polymorphism and messenger RNA (mRNA) expression of LPL with IMF deposition in the chest muscle (CM) and leg muscle (LM) of TYC and BOC. Sixty TYCs and 60 BOCs were raised from 1 d and slaughtered by avascularization at their slaughtering age. IMF contents of the CM and LM in the BOC were markedly higher than those in the TYC. Three genotypes following AA, AB and BB were found by the method of polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). The synonymous mutation C12315T was detected. The content of IMF with the AA genotype was significantly higher than the AB genotype in the LM of TYC. The mRNA expression both of CM and LM in BOC was prominently higher than those in TYC, and there was a positive significant correlation between LM and CM in both BOC and TYC. These results suggested that the SNPs polymorphism and mRNA expression of the LPL gene might be helpful for selective breeding in IMF of the chicken.

Evaluation of physiological and molecular responses to acute heat stress in two chicken breeds

High environmental temperatures are a foremost concern affecting poultry production; thus, understanding and controlling such conditions are vital to successful production and welfare of poultry. In view of this, a completely randomized design with a 2 × 2 factorial arrangement involving two local strains (Kirin chicken (KC) and Three-yellow chicken (TYC)) and two temperature groups (normal/control = 30 ± 2 °C and acute heat stress (AHS) = 35 ± 1 °C for 8-h with 70% humidity) was used to assess the main regulatory factors such as heat shock protein (HSP70) gene, cytokine genes (IL-1β, IL-6, IL-10), muscle development gene (IGF-1) and tissue histopathological changes. At 56 days old, the temperatures of the comb (CT), feet (FT), eyelid (ET) and rectal (RT) from each group were taken thrice at 0, 2, 4 and 8-h during AHS, and 1 and 3-h recovery period after AHS. At 80 days old, the slaughter weight was also analyzed. The CT and ET of the AHS groups increased during the 8-h trial, while the RT of both strains decreased significantly at 4 h but increased at 8 h in the TYC group. All temperature recordings dropped in the AHS groups of both strains during the recovery period. The results revealed that the mRNA expression of HSP70 in the liver was higher in the heat-stressed group of both strains compared to the control. The expression of HSP70 was shown in the AHS-KC group to be significantly high compared to the control (P < 0.05). Moreover, the IGF1 gene in the liver, breast muscle and leg muscle was downregulated in the AHS-TYC group compared to the control (P < 0.05), although that in the AHS-KC was downregulated in the breast muscle. The mRNA expression of spleen IL-1β significantly decreased in the AHS-TYC group (P < 0.01), whereas that of the AHS-KC had no significant difference (P > 0.05). The mRNA expression of spleen IL-6 and IL-10 was increased in the AHS-KC group but did not exhibit obvious changes in the AHS-TYC. Correspondingly, the histopathological examinations revealed tissue injury in the AHS groups of both strains, with the TYC strain experiencing more severe changes. The final live and carcass weights showed a significant enhancement in the treatments (P < 0.01 and P < 0.05, respectively) and treatment×strain interaction (P < 0.05) with breast muscle rate significantly reducing among the treatments (P < 0.01) at 80 days. In conclusion, the differential response to AHS after physiological, molecular and immune response portrays KC to have better thermal tolerance than the TYC.

An outbreak in three-yellow chickens with clinical tumors of high mortality caused by the coinfection of reticuloendotheliosis virus and Marek’s disease virus: a speculated reticuloendotheliosis virus contamination plays an important role in the case

Both reticuloendotheliosis and Marek's disease are neoplastic diseases of chickens caused by reticuloendotheliosis virus (REV) and Marek's disease virus (MDV), respectively. The infection of REV or MDV may lead to clinical tumors and also result in immunosuppression and easily allow secondary infection by other pathogens. Here, we investigated a breeder flock of three-yellow chickens in southern China that had been vaccinated with CVI988/Rispens at hatching and had experienced depression, weakness, reduction in weight gain, and an increased death rate after 120 d of age. The morbidity and mortality were 20% and 10%, respectively, at 140 d of age when this infection was diagnosed. The necropsy of the birds revealed significant tumor-like lesions in the heart, liver, spleen, and ceca. Peripheral blood lymphocytes and tumor-like tissues were sampled for PCR detection and for histopathological observation, for virus isolation and the subsequent immunofluorescent assay on the cell cultures and for gene sequencing of the isolated viruses. A REV isolate GX18NNR1 and a MDV isolate GX18NNM5 were both recovered from the sampled bird. Further phylogenetic analysis based on the env gene of REV and the meq gene of MDV demonstrated that GX18NNR1 was closely related to the reference REV strain MD-2, which was isolated from a contaminated commercial turkey herpesvirus vaccine. In addition, the GX18NNM5 was found to belong to the Chinese very virulent MDV strains' cluster. The coinfection of REV and MDV may contribute to tumor outbreaks with high morbidity and mortality in three-yellow chicken flocks.

Genomic Analysis Reveals Strong Signatures of Selection in Guangxi Three-Yellow Chicken in China

Genomic Analysis Reveals Strong Signatures of Selection in Guangxi Three-Yellow Chicken in China

Complete genome sequence of a novel recombinant avian leukosis virus isolated from a three-yellow chicken.

In this study, an avian leukosis virus (ALV) strain (GX-2020-01) was isolated from a three-yellow chicken, and its complete genome was 7570bp long with the typical organization "5'LTR-gag-pol-env-3'LTR." Phylogenetic analysis and sequence comparison revealed that it belongs to the ALV-J subgroup. However, the LTR region of GX-2020-01 is highly similar to that of reference strains of ALV-K/E (96.61%-97.10%), demonstrating that this novel isolate is a natural recombinant. The replication efficiency of GX-2020-01 was significantly lower than the previously isolated ALV-J strain (NX0101), indicating that the recombination event might have resulted in slower virus replication, making it harder for it to be detected through routine testing.

The study on robot eviscerating technology and visceral contour recognition based on image processing for poultry

In this study, robot eviscerating technology was used in the poultry slaughter industry. The poultry viscera were grabbed by the manipulation with the guidance of machine vision technology. A machine-vision-based method of locating the viscera of poultry carcasses was described. The threshold segmentation method, sobel operator and image operations were used to segment the images of poultry viscera. Subsequently, the visceral contour was extracted and its position was detected for common poultry such as Three-Yellow Chicken. The identification rates of this visceral contour recognition system were 95.3%, suggesting that the proposed image recognition algorithm could achieve the accuracy required for poultry visceral contour detection. Therefore, the traditional artificial poultry eviscerating would be replaced by robot eviscerating technology, and the new technology exhibited higher automation program and better eviscerated effect, the location prediction of viscera and the positioning of the machine vision system could be designed, the new technical means were provided for subsequent research on the conveyor chain of poultry eviscerated.

Differential Regulation of chTLR3 by Infectious Bursal Disease Viruses with Different Virulence In Vitro and In Vivo.

Toll-like receptor 3 (TLR3) is one of the TLRs whose ligand is double-stranded RNA (dsRNA). Infectious bursal disease virus (IBDV) is a dsRNA virus that could be recognized by TLR3. The purpose of this study was to determine the role of the virulence of IBDV on the expression of chicken TLR3 (chTLR3). For this purpose, the levels of chTLR3 expression and its downstream effectors, Interferon β (IFN-β) and Interleukin 8 (IL-8), were detected and analyzed after infection of IBDV field isolates with differential virulence in vitro (chicken embryo fibroblast and/or chicken peripheral blood mononuclear cells) and in vivo (commercial Three-Yellow chicken). The results showed that chTLR3 was activated by IBDV, resulting in the expression of antiviral IFN-β and chemokine IL-8. The expression of chTLR3, IFN-β, and IL-8 correlated well with the virulence of IBDV as the more virulent the IBDV strain that was used, the more pronounced was the expression of chTLR3, IFN-β, and IL-8. These results suggest that chTLR3 is involved in the pathogenesis of IBDV in commercial chickens and its downstream effectors (IFN-β and IL-8) might play an important role in this process.

Correlation of the A-FABP Gene Polymorphism and mRNA Expression with Intramuscular Fat Content in Three-Yellow Chicken and Hetian-Black Chicken

ABSTRACTThe adipocyte-type fatty acid-binding protein (A-FABP) is considered a candidate gene for fat metabolism; thus, it affects fat deposition in chickens. The present study was designed to examine the polymorphism and mRNA abundance of the A-FABP gene with intramuscular fat (IMF) in the pectoralis muscles (PM) and leg muscles (LM) of Three-yellow Chicken (TYC) and Hetian-black Chicken (HTBC). In total, 60 TYCs and 60 HTBCs were sacrificed using exsanguination at market age. The IMF contents of the PM and LM in the HTBC were significantly higher than those in the TYC. Three genotypes of the A-FABP gene first exon, AA, AB, and BB, were examined by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), and a C51 T mutational site, which is a silent substitution mutation, was revealed. The IMF contents of the AA genotype in the PM of the HTBC were significantly higher than those in the AB genotype; thus, the C51 T mutable site is a gene marker for selecting a higher IMF content in the PM of the HTBC. The relative expression of the A-FABP mRNA in the LM of the HTBC, which was measured by quantitative real-time PCR, was significantly higher than in the TYC. A significantly positive association was detected between A-FABP expression with the IMF contents of the PM and LM of both the TYC and the HTBC. These results provide basic data that might be helpful to further research the role of the A-FABP gene in fat deposition and fatty acid metabolism in chickens.

Complete mitochondrial genome of the Wuhua three-yellow chicken (Gallus gallus domesticus)

Wuhua three-yellow chicken is a native breed of Guangdong Province in China. The complete mitochondrial DNA (mtDNA) genome presented here was the first assemble of Wuhua three-yellow chicken, which was determined through the polymerase chain reaction-based method. The complete mitogenome was 16,784 bp in length, with the nucleotide composition of 30.29% for A, 23.75% for T, 32.48% for C and 13.48% for G, and exhibited the typical mitochondrial structure, including 2 rRNA genes, 13 protein-coding genes, 22 tRNA genes and a non-coding control region.

Genetic diversity and relationship between genetic distance and geographical distance in 14 Chinese indigenous chicken breeds and red jungle fowl

Genetic diversity and the relationship between genetic distance and geographical distance in red jungle fowl and 14 Chinese indigenous chicken breeds were evaluated using 29 microsatellite loci. The number of alleles per locus ranged from 2 to 25 and the average expected heterozygosity and PIC of all loci were 0.6683 and 0.50, respectively. The average number of alleles per locus ranged from 3.41 in Gushi chicken breed to 6.28 in Wannan Three-yellow chicken breed. The overall expected heterozygosity of 15 Chinese chicken breeds was 0.6686 ± 0.0254 and all breeds showed relatively large heterozygosity. The average of genetic differentiation among populations was 16.4% (&lt;I&gt;P&lt;/I&gt; &lt; 0.001). Red jungle fowl and Gushi chicken had distant genetic relationship from other breeds, while Huainan Partridge and Tibetan chicken were more closely related with other breeds. The results did not provide enough support for a significant correlation between the genetic and geographical pair-wise distances.