This study examined the influence of medial implant location in three-unit posterior cantilever fixed partial dentures (FPDs) on stress distribution in mandibular bone surrounding two implants. A three-dimensional finite element model that included three-unit FPD and two cylindrical-type implants (4 mm in diameter and 10 mm in length) osseointegrated in the posterior mandible, was digitized. Five different models were created according to the medial implant location between the missing second premolar and the first molar location. The distal implant was fixed at the missing second molar location. Oblique bite force of 100 N at 30 degrees buccal to the vertical direction was directed on each of three artificial teeth, respectively and simultaneously, while the lower surface of the mandible was fixed. The maximum equivalent stress in the cortical and the trabecular bone generally increased as the medial implant shifted to a distal position. Under the simultaneous bite force, relatively low maximum stresses within the cortical bone: between 55 MPa and 57 MPa, were shown in the models with the medial implant placed within the range of one implant diameter from the most medial position, while higher maximum stresses: between 64 MPa and 73 MPa, were demonstrated with more distally placed medial implants. The results suggest that reasonably low mechanical stress in the surrounding bone may be assured when the medial implant is placed in the range between the missing second premolar position and one implant diameter distal from that location.