Three-dimensional Platform Research Articles

Redox-responsive functionalized hydrogel marble for the generation of cellular spheroids

Liquid marbles (LMs) have recently shown a great promise as microbioreactors to construct self-supported aqueous compartments for chemical and biological reactions. However, the evaporation of the inner aqueous liquid core has limited their application, especially in studying cellular functions. Hydrogels are promising scaffolds that provide a spatial environment suitable for three-dimensional cell culture. Here, we describe the fabrication of redox-responsive hydrogel marbles (HMs) as a three-dimensional cell culture platform. The HMs are prepared by introducing an aqueous mixture of a tetra-thiolated polyethylene glycol (PEG) derivative, thiolated gelatin (Gela-SH), horseradish peroxidase, a small phenolic compound, and human hepatocellular carcinoma cells (HepG2) to the inner aqueous phase of LMs. Eventually, HepG2 cells are encapsulated in the HMs then immersed in culture media, where they proliferate and form cellular spheroids. Experimental results show that the Gela-SH concentration strongly influences the physicochemical and microstructure properties of the HMs. After 6 days in culture, the spheroids were recovered from the HMs by degrading the scaffold, and examination showed that they had reached up to about 180μm in diameter depending on the Gela-SH concentration, compared with 60μm in conventional HMs without Gela-SH. After long-term culture (over 12 days), the liver-specific functions (secretion of albumin and urea) and DNA contents of the spheroids cultured in the HMs were elevated compared with those cultured in LMs. These results suggest that the developed HMs can be useful in designing a variety of microbioreactors for tissue engineering applications.

Traversing behavior of tumor cells in three-dimensional platforms with different topography.

Three-dimensional polydimethylsiloxane platforms were developed to mimic the extracellular matrix with blood vessels by having scaffolds with micropatterns, porous membrane and trenches. Precisely controlled physical dimensions, layouts, and topography as well as different surface chemical treatments were applied to study their influences on nasopharyngeal carcinoma cell (10–15 μm in diameter) migration in mimicked platforms over 15-hour of time-lapse imaging. By placing the pores at different distance from the edges of the trenches, pores with different trench sidewall exposures and effective sizes were generated. Pores right next to the trench sidewalls showed the highest cell traversing probability, most likely related to the larger surface contact area with cells along the sidewalls. Straight grating oriented perpendicular to trenches below the top layer increased cell traversing probability. Pore shape as well as pore size influenced the cell traversing probability and cells could not traverse through pores that were 6 μm or less in diameter, which is much smaller than the cell size. Trench depth of 15 μm could induce more cells to traverse through the porous membrane, while shallower trenches impeded cell traversing and longer time was needed for cells to traverse because 3 and 6 μm deep trenches were much smaller than cell size which required large cell deformation. Hydrophobic surface coating on the top layer and fibronectin in pores and trenches increased the cell traversing probability and reduced the pore size that cells could traverse from 8 to 6 μm, which indicated that cells could have larger deformation with certain surface coatings.

Remote nongenetic optical modulation of neuronal activity using fuzzy graphene

The ability to modulate cellular electrophysiology is fundamental to the investigation of development, function, and disease. Currently, there is a need for remote, nongenetic, light-induced control of cellular activity in two-dimensional (2D) and three-dimensional (3D) platforms. Here, we report a breakthrough hybrid nanomaterial for remote, nongenetic, photothermal stimulation of 2D and 3D neural cellular systems. We combine one-dimensional (1D) nanowires (NWs) and 2D graphene flakes grown out-of-plane for highly controlled photothermal stimulation at subcellular precision without the need for genetic modification, with laser energies lower than a hundred nanojoules, one to two orders of magnitude lower than Au-, C-, and Si-based nanomaterials. Photothermal stimulation using NW-templated 3D fuzzy graphene (NT-3DFG) is flexible due to its broadband absorption and does not generate cellular stress. Therefore, it serves as a powerful toolset for studies of cell signaling within and between tissues and can enable therapeutic interventions.

Application of Power Load Forecasting in Urban Distribution Network Planning Based on 3D Real Scene Platform

The 3D real-view platform technology builds a power load forecasting platform in urban distribution network planning. The completion of this system can provide integrated support for power load forecasting in power distribution line planning, and further provide a three-dimensional foundation platform for power load forecasting analysis and calculation software in power distribution planning. Urban distribution network planning is an important task for power supply companies. The quality of its construction directly affects the development of urban economy and the improvement of people’s quality of life. This paper combines the years of work experience to introduce the importance of power distribution network planning, which optimizes the structure of the power distribution network, which can ensure the safe operation of the power grid, reduce power loss, improve power supply reliability, and accurately predict power loads. To meet the needs of urban power load development.

Development of coal geological information technologies in China

The history of coalfield geology and mine geology IT applications is over 30 years, which is gaining remarkable achievements. This paper sheds light on the development of geological and surveying spatial management information systems. Specifically, this study proposed the development trend, system structure and function design and sub-systems of the new generation of geological and surveying spatial management information system by benefitting from the recent advancements in computer and spatial information technologies. The sub-systems include the “One Map” online collaborative (2D-GIS) management platform, the geological survey mapping collaborative management system, the integrated service system based on “one map”, the three-dimensional dynamic geological model system, the three-dimensional transparent management platform, and water prevention and control information management system. This work is an important foundation to realize the IT information and intelligent management of coal industry. The successful development and application of the new generation of geological and surveying spatial management information system will provide the dynamic data support for the online decision-making of the intelligent coal mining.

Implementation of Multiple Regression Technique for Detection of Gait Asymmetry Using Experimental Gait Data

This study reports the implementation of different regression (multivariate and step-wise) techniques towards determining the gait asymmetry and comparison with the symmetry indices (SI) values. To predict the gait asymmetry between two different legs, a set of gait trials (thirty-five participants) via a three-dimensional motion capture setup and force platform available at the parent institute, has been acquired. Two separate regression fit models are prepared to indicate the significant gait parameters for the right and left legs utilizing the recorded foot data. The significant sets of coefficients for the right and left leg parameters are compared with the SI values to validate the gait asymmetry. The calculated mean SI from the experimental results correspond to the predicted regression model responses, and 18 of the 27 regression fits present different sets of significant coefficients for the right and left leg parameters. The regression fits show gait parameter dependency on the different sets of predictor variables. The method can be adopted for different patient data sets to detect the influence of the causative factors on pathologic gait.

Do Lower-Limb Kinematic and Kinetic Asymmetries Transfer Across Sprint Running and Countermovement Jumps in University Rugby Union Players?

The aim of this study was to examine if lower-limb kinetic and kinematic asymmetries are transferred between sprint running and countermovement jumps in a group of university Rugby Union players. Eight university Rugby Union players (20.3 ± 1.6 years) participated in the study. Three-dimensional kinematic and force platform data recorded sprint runs and countermovement jumps. Across the two movements there was a substantial and moderate level of agreement for the ankle range of motion and peak normalized ground reaction force, respectively. No significant differences were observed between interlimb kinematic and kinetic variables at the group level. Lower-limb asymmetries may be transferred across dynamic movements and are present at the individual level.

Three-Dimensional Explant Platform for Studies on Choroid Plexus Epithelium.

The choroid plexus (CP) plays a major role in controlling the entry of substances and immune cells into the brain as it forms the blood-cerebrospinal fluid barrier (BCSFB) in the brain ventricles. Dysregulated immune cell trafficking through the epithelial cell (EC) layer of CP is central for the pathogenesis of infectious diseases in the brain and many neurodegenerative disorders. In vitro studies elucidating the function of the CP have so far been limited to the monolayer culture of CP ECs. To mimic immune cell migration across the CP barrier, a three-dimensional model would be advantageous. Here, we present an in vitro platform for studies of the immune cell trafficking based on CP explants/organoids. The explants were generated from fragments of mouse CPs in Matrigel, where the cells formed luminal spaces and could be maintained in culture for at least 8 weeks. We demonstrate expression of the major CP markers in the explants, including transthyretin and aquaporin 1 as well as ZO1 and ICAM-1, indicating a capacity for secretion of cerebrospinal fluid (CSF) and presence of tight junctions. CP explants displayed CP-like cell polarization and formed an intact EC barrier. We also show that the expression of transthyretin, transferrin, occludin and other genes associated with various functions of CP was maintained in the explants at similar levels as in native CP. By using dendritic cells and neutrophils, we show that the migration activity of immune cells and their interactions with CP epithelium can be monitored by microscopy. Thereby, the three-dimensional CP explant model can be used to study the cellular and molecular mechanisms mediating immune cell migration through CP epithelium and other functions of choroid EC. We propose this platform can potentially be used in the search for therapeutic targets and intervention strategies to improve control of (drug) substances and (immune) cell entry into the central nervous system.

A naval damage incident recoverability toolset: Assessing naval platform recoverability after a fire event

Naval platform survivability is a key enabler to ensure maritime warfighting capability. Therefore, assessment of naval platform recoverability, after a damage event, is critical to assure platform survivability in a warfighting environment. To support such an assessment, an innovative modelling and simulation capability, known as the Naval Damage Incident Recoverability Toolset (NavDIRecT) is being developed. NavDIRecT is being designed as a component-based, open architecture providing the necessary framework to allow analysts to integrate domain models of their choosing. NavDIRecT will facilitate analysis of warfighting and peacetime damage events using a variety of mathematical models, thereby avoiding the limitations of other survivability assessment techniques. Development of NavDIRecT is exemplified by integrating the human movement simulator, maritimeEXODUS, the fire simulation environment, SMARTFIRE, and a three-dimensional naval platform model. NavDIRecT will enable analysis of crew interaction with damage events, thereby allowing acquisition programs and mission planners to examine platform survivability with respect to mission capability requirements. The impetus for NavDIRecT development is for assessment of naval platform survivability and mission success; however, the tools and techniques are equally suitable for use in incident management, training, and analysis of merchant and commercial shipping in accordance with the Safety of Life at Sea (SOLAS).

Virtual reality with three-dimensional image guidance of individual patients' vessel anatomy in laparoscopic distal pancreatectomy.

Three-dimensional virtual endoscopy (3DVE) has the potential advantage of enhanced anatomic delineation and spatial orientation during laparoscopic procedures. In the present study, we aimed to evaluate the impact of 3DVE guidance in laparoscopic distal pancreatectomy (LDP). Thirty-eight patients presenting to our hospital with a variety of pancreatic tumors underwent preoperative computed tomography scanning to clearly define the major peripancreatic vasculature and correlate it with a 3DVE system (SYNAPSE VINCENT: Fujifilm Medical, Tokyo, Japan). This map served as the guide during preoperative planning, surgical education, and simulation and as intraoperative navigation reference for LDP. Operative records and pathological findings were analyzed for each procedure. Operative parameters were compared between the 38 patients in this study and 8 patients performed without 3DVE guidance at our institution. The 3DVE navigation system successfully created a preoperative resection map in all patients. Relevant peripancreatic vasculature displayed on the system was identified and compared during the intervention. The mean blood loss in LDP performed under 3DVE guidance versus LDP without 3DVE was 168.5 +/- 347.6 g versus 330.0 +/- 211.4 g, p = 0.008 while and the operative time was 171.9 +/- 51.7 min versus 240.6 +/- 24.8 min, p = 0.001. 3DVE in conjunction with a "laparoscopic eye" creates a preoperative and intraoperative three-dimensional data platform that potentiallyenhances the accuracy and safety of LDP.

Cytoarchitecture of the mouse brain by high resolution diffusion magnetic resonance imaging

MRI has been widely used to probe the neuroanatomy of the mouse brain, directly correlating MRI findings to histology is still challenging due to the limited spatial resolution and various image contrasts derived from water relaxation or diffusion properties. Magnetic resonance histology has the potential to become an indispensable research tool to mitigate such challenges. In the present study, we acquired high spatial resolution MRI datasets, including diffusion MRI (dMRI) at 25 ​μm isotropic resolution and quantitative susceptibility mapping (QSM) at 21.5 ​μm isotropic resolution to validate with conventional mouse brain histology. Diffusion weighted images (DWIs) show better delineation of cortical layers and glomeruli in the olfactory bulb than fractional anisotropy (FA) maps. However, among all the image contrasts, including quantitative susceptibility mapping (QSM), T1/T2∗ images and DTI metrics, FA maps highlight unique laminar architecture in sub-regions of the hippocampus, including the strata of the dentate gyrus and CA fields of the hippocampus. The mean diffusivity (MD) and axial diffusivity (AD) yield higher correlation with DAPI (0.62 and 0.71) and NeuN (0.78 and 0.74) than with NF-160 (−0.34 and −0.49). The correlations between FA and DAPI, NeuN, and NF-160 are 0.31, −0.01, and −0.49, respectively. Our findings demonstrate that MRI at microscopic resolution deliver a three-dimensional, non-invasive and non-destructive platform for characterization of fine structural detail in both gray matter and white matter of the mouse brain.

Safety and Performance of Offshore Platforms Subjected to Repeated Earthquakes

In this work, a systematic study is conducted on the behavior of three-dimensional offshore oil/natural gas platforms under the action of seismic sequences. Such repeated earthquakes result in a noteworthy accumulation of damage in a platform since, in many cases, any rehabilitation process between any two or more successive ground motions cannot be essentially materialized because of lack of time. Conversely, in the past, the seismic response of offshore platforms has been exclusively investigated for the case of single earthquakes. In this study, two three-dimensional platforms are examined, where the first one is assumed to be completely constrained at its base (fixed boundary conditions), while the second one is founded in deformable soil with the aid of long piles. These structures are subjected to real seismic sequences which have been recorded by the same station in a short period of time. Additionally, the platforms under consideration are also subjected to artificial seismic sequences. In this study, we found that sequential earthquakes have a significant effect on the response of these special structures, and this finding should be taken into account in their design.

Blood Serum Cytokines Induce Changes in Blood‐Brain Barrier Permeability and Gene Expression in an In Vitro Blood‐Brain Barrier Model

The blood‐brain barrier (BBB), a semi‐permeable interface between the brain and the blood, is a key player in the of development and progression of brain‐related illnesses such as traumatic brain injury (TBI), Alzheimer’s disease (AD), multiple sclerosis (MS), and schizophrenia (SCZ). With these disorders, pathologic BBB leakiness is caused by changes in the cellular milieu on the brain‐side as well as on the blood‐side of the BBB. On the brain‐side, injury to neurons, astrocytes, microglia, and pericytes can lead to increased BBB leakiness, while on the blood‐side, pathophysiologic levels of circulating factors such as hormones, inflammatory cytokines, and immune cells can significantly alter BBB leakiness.The objective of this study was to discover novel biomarkers of BBB leakiness using the in vitro three‐dimensional Neurovascular Unit (3D NVU; Research Triangle Institute International, Durham, NC) to examine the impact of human blood serum on transcriptional regulation and function of the BBB. The 3D NVU integrates endothelial, neuronal, astrocytic, and microglial cells into a three‐dimensional platform, and allows for the assessment of BBB permeability (transendothelial electrical resistance (TEER, Ω cm2)). Blood serum of secondary progressive MS patients (two men, two women) and four healthy individuals (two men, two women) were purchased from PrecisionMed, Inc. (Solana Beach, CA), and diluted to 40% with cell culture media for testing. TEER of the 3D NVU was recorded immediately prior to serum exposure (baseline), and again at 24 hours after serum treatment. At the end of the experiment, cells of the 3D NVU were collected, and expression of 84 MS‐related genes were measured by quantitative RT‐PCR. BBB permeability was calculated as TEERSerum – TEERBaseline. The protein levels of 100 serum cytokines were measured in undiluted serum samples by membrane‐based sandwich immunoassay. Multiple regression analysis was used to determine the relationship among TEER, serum cytokine levels, and BBB gene expression.We found that no single serum cytokine was a strong predictor of BBB permeability, however, serum‐induced changes in expression of chemokine ligand 12 (Ccl12/Mcp‐5), chemokine ligand 7 (Ccl7), hexosaminidase subunit beta (Hexb), interleukin 13 (Il13), interleukin 1 beta (Il1b), protein tyrosine phosphatase non‐receptor type 11 (Ptpn11), and vascular adhesion molecule 1 (Vcam1) were strong and significant predictors of BBB permeability (R2’s ≥ 0.76, P’s ≤ 0.004). Some of these genes (Il1b, Hexb and Vcam1) have been shown previously to play a role in regulation of BBB function, however, genes such as Ptpn11 have not yet been implicated in BBB permeability. This preliminary study demonstrates that the 3D NVU can be used to elucidate novel, physiologically relevant biomarkers of BBB‐related neurological and psychological disorders. In the future, we plan to perform RNA‐sequencing to examine a broader range of differential gene expression in our study.Support or Funding InformationFunded by TATRC AMEDD Advanced Medical Technology Initiative grant. The views expressed in this abstract are those of the authors and do not reflect the official policy or position of the Department of the Army, Department of Defense, or the U.S. Government.

Review of Three-Dimensional Liquid Chromatography Platforms for Bottom-Up Proteomics.

Proteomics is a large-scale study of proteins, aiming at the description and characterization of all expressed proteins in biological systems. The expressed proteins are typically highly complex and large in abundance range. To fulfill high accuracy and sensitivity of proteome analysis, the hybrid platforms of multidimensional (MD) separations and mass spectrometry have provided the most powerful solution. Multidimensional separations provide enhanced peak capacity and reduce sample complexity, which enables mass spectrometry to analyze more proteins with high sensitivity. Although two-dimensional (2D) separations have been widely used since the early period of proteomics, three-dimensional (3D) separation was barely used by low reproducibility of separation, increased analysis time in mass spectrometry. With developments of novel microscale techniques such as nano-UPLC and improvements of mass spectrometry, the 3D separation becomes a reliable and practical selection. This review summarizes existing offline and online 3D-LC platforms developed for proteomics and their applications. In detail, setups and implementation of those systems as well as their advances are outlined. The performance of those platforms is also discussed and compared with the state-of-the-art 2D-LC. In addition, we provide some perspectives on the future developments and applications of 3D-LC in proteomics.

Hyaluronan‐based hydrogels as versatile tumor‐like models: Tunable ECM and stiffness with genipin‐crosslinking

Three-dimensional (3D) biomimetic cell culture platforms offer more realistic microenvironments that cells naturally experience in vivo. We developed a tunable hyaluronan-based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs). For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP). Then, we thoroughly characterized our substrates before testing them with glioblastoma and breast cancer cells, and thereafter with endothelial cells. Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent. While PLL-treated hydrogels were not favorable to the culture of any glioblastoma cell lines, they enhanced the proliferation of breast cancer cells in a stiffness-dependent manner. Contrary to type III collagen, type IV collagen pre-treated hydrogels supported the proliferation of glioblastoma cells. The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.

Abstract P1-04-01: Breast cancer patient derived scaffolds as a platform for studying cancer promoting properties of the microenvironment

Abstract Despite great efficiencies of today’s anti-cancer therapies in breast cancer medicine, recurrences and metastasis still remains a major challenge. The tumor initiating population of cancer stem cells (CSC) is believed to promote metastasis and drug resistance, suggesting that selectively targeting CSC and their niche may be a potential beneficial therapeutic strategy. However, specific driving factors in the microenvironment influencing the CSC niche remains unknown. To study the influence of specific cancer microenvironments, we have developed a novel three-dimensional cell culture platform, using cell-free patient derived scaffolds (PDS) from decellularized breast cancer tumors. We confirmed that our novel patient derived scaffold (PDS) recapitulates the native microenvironment where the cancer cells originate and, retains their biological properties. Our data demonstrate that culturing breast cancer cell lines in PDSs promotes CSC and EMT-like properties and decreases proliferation, similarly to in vivo conditions. Additionally, global gene expression profiling revealed that PDS cultures exhibit transcriptional patterns more similar to patient derived xenografts than to traditional monolayer cultures. Moreover, we analyzed the expression of cells cultured in 108 PDSs made from breast cancer tumors with clinical follow-up data, using a gene panel representative of relevant breast cancer related processes. This revealed that expression changes of several EMT markers and other CSC related genes in the PDS cultured cancer cells were correlated to clinical parameters such as grade, lymph node metastasis, patient survival and disease recurrences. Altogether, our data shows that PDS can reveal unique additional information about the malignancy-inducing properties of specific tumor microenvironments, potentially provides a complementary diagnosis tool for breast cancer tumors in vitro, and is a promising platform for drug screening of anti-cancer therapies. Citation Format: Elena Garre, Anna Gustafsson, Maria del Carmen Leiva-Arrabal, Paul Fitzpatrick, Pernilla Gregersson, Anders Ståhlberg, Göran Landberg. Breast cancer patient derived scaffolds as a platform for studying cancer promoting properties of the microenvironment [abstract]. In: Proceedings of the 2019 San Antonio Breast Cancer Symposium; 2019 Dec 10-14; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2020;80(4 Suppl):Abstract nr P1-04-01.

Liposomal Spherical Nucleic Acid Scaffolded Site-Selective Hybridization of Nanoparticles for Visual Detection of MicroRNAs.

In this study, the advanced liposomal spherical nucleic acid (L-SNA) is exploited for the first time to establish a spherical, three-dimensional biosensing platform by hybridizing with a set of nanoparticles. By hydrophilic and hydrophobic interactions as well as programmable base-pairing, red-emission quantum dots (QDs), green-emission QDs, and gold nanoparticles (AuNPs) are encapsulated into the internal aqueous core, the intermediate lipid bilayer, and the outer SNA shell, respectively, producing an L-SNA-nanoparticle hybrid. As a result of the site-selective encapsulation, the hybrid constitutes a liposomal fluorescent "core-resonance energy transfer" system surrounded by a SNA shell, as is imaged at the single-particle resolution by confocal microscopy. With the outer SNA shell as three-dimensional substrate for duplex-specific nuclease target recycling reaction, the hybrid is capable of amplified detection of microRNAs, featuring one target to many AuNP-manipulated, dual-emission QD-based ratiometric fluorescence. More importantly, the ratiometric fluorescence facilitates the hybrid to visualize microRNAs with remarkably high resolution, which is exemplified by traffic light-type transition in fluorescence color for diagnosing circulating microRNAs in clinical serum samples. Substantially, the controllable hybridization with functional nanoparticles opens an avenue for the exciting biomedical applications of liposomal spherical nucleic acids.

A high-throughput three-dimensional cell culture platform for drug screening

Traditional two-dimensional (2D) cell cultures lack the extracellular matrix (ECM)-like structure or dynamic fluidic microenvironment for cells to maintain in vivo functionality. Three-dimensional (3D) tissue scaffolds, on the other hand, could provide the ECM-like microenvironment for cells to reformulate into tissue or organoids that are highly useful for in vitro drug screening. In this study, a high-throughput two-chamber 3D microscale tissue model platform is developed. Porous scaffolds are selectively foamed on a commercially available compact disk using laser. Perfusion of cell culture medium is achieved with centrifugal force-driven diffusion by disk rotation. Experimental studies were conducted on the fabrication process under various gas saturation and laser power conditions. Cell cultures were performed with two types of human cell lines: M059K and C3A-sub28. It is shown that the structure of microscale porous scaffolds can be controlled with laser foaming parameters and that coating with polydopamine these scaffolds are inducive for cell attachment and aggregation, forming a 3D network. With many such two-chamber models fabricated on a single CD and perfusion driven by the centrifugal force from rotation, the proposed platform provides a simple solution to the high-cost and lengthy drug development process with a high-throughput and physiologically more relevant tissue model system.

Crowd Evacuation Behaviour Modeling and Simulation in 3D Platform

Crowd simulation is an active research domain and is crucial for simulating crowd behaviour in certain condition such as normal or panic situation. The simulation is to show the interaction between the individual in a crowd. Nowadays, there are many kinds of scenarios as well as simulation softwares that can be adapted to simulate a crowd simulation such as during emergency situation e.g. building evacuation. Crowd simulation in three-dimensional platform is fairly important in order to have a more realistic looks and movement of the crowd in one particular environment. The evacuation simulation is useful for the crowd in one confinement to seek for a safe exit path in shortest time possible and thus increase the occupant’s safety. The evacuation time is said to be in safe condition if all the evacuees successfully can get through the exit in minimal time. To aid in minimal exit time, the concept of faster-is-slower (bottleneck) must be solved as it can lead to more waiting time or delay during evacuation process. In this paper, it will discuss about the crowd simulation behavior, crowd simulation based on agent-based model, existing crowd simulation tools and the result of simulating the three-dimensional (3D) crowd evacuation time based on a number of exits variation in panic situation. The tools used to carry out the experiment is Anylogic software whereby the results show that it adheres to shorter evacuation time when the number of exit increases. The 3D layout design was following the original layout the faculty’s lower ground floor where the classrooms are mostly resided. The simulation is useful in order to estimate of evacuation time with different total number of exits to alleviate the faster-is-slower effect in case of any emergency situation happens at the faculty building.

Meteolakes: An operational online three-dimensional forecasting platform for lake hydrodynamics

Environmental management depends on high-quality monitoring and its meaningful interpretation. The combination of local weather dynamics, regional anthropogenic stresses and global environmental changes make the evaluation of monitoring information in dynamic freshwater systems a challenging task. While the lake ecosystems gather many complex biogeochemical interactions, they remain constrained by the same physical environment of mixing and transport. It is therefore crucial to obtain high-quality physical system insight. Three-dimensional hydrodynamic models are perfectly suited for providing such information. However, these models are complex to implement, and their use is often limited to modellers. Here, we aim to provide model output via a user-friendly platform to a broad audience ranging from scientists to public and governmental stakeholders.We present a unified approach merging the apparently diverse interests through meteolakes.ch, an online platform openly disseminating lake observations and three-dimensional numerical simulations in near real-time with short-term forecasts and data assimilation. Meteolakes is scalable to a broad range of devices, modular and distributed, hence allowing its expansion to other regions and hardware infrastructures. Since 2016, the platform has continuously provided timely synoptic lake information to more than 250,000 users. This web-based system was built not only to provide guidance to scientists in the design and analysis of field experiments and to foster interdisciplinary lake studies, but also to assist governmental agencies and professionals in the long-term policy and planning of water resources management. Finally, our system aimed at promoting awareness and understanding of the complexity of lakes and providing information to the public through user-friendly interfaces. This article details the design and operation of such a platform and its products. Applications are demonstrated by examples of a recent upwelling and a storm event. Both cases illustrate how Meteolakes help scientists in their quest for process understanding as well as water professionals and civil society in providing specific warnings.